Cucsa.162320.1 (mRNA) Cucumber (Gy14) v1

NameCucsa.162320.1
TypemRNA
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionPlant protein of unknown function (DUF247)
Locationscaffold01144 : 1997775 .. 1999325 (-)
Sequence length1318
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAAACAATCAGTGAGATTGATCCAAAAGTTTGTGGTTATGTTGTGAGTTCTATTGACAAAATATTGGAAGAACGGTTTCGAGTTGATACAAAATGTCACAATATATATCAACTTTCAAAAGAGCTACGCGAGATGAATCATGCATAAAGCCTATATCCCTCAATTCATTTCCATCGGTCCTTTTCACCATGGCACTCGAAATGATTTGACAGCTAATGAACATTATAAGATTCAAGGTTTTAATAATTTTCTACATCGTATAAATATTAATTATAAGCAGGTTGAATCATTGAAGGAGCTTGTGAAAAAATGTCATGGTTGGATGAAAGATGCTTGGAATTGTTATGCAGAACCAATAGAGATGGAGGAGAAGACGTTTGTTTTAATGATGCTTGTGGATGCTTGTTTCATACTCGAGTTTTTTATACTACTAATTGATCCCCTTGGTCATGGTCGTAATCTATTTGGAGTAGATCATTTTTTTTCAAATTCAAGACATTGTAGACGTCTCATTCTACCACGGAAAATTATTTGAAATACTTGTTGACTTGATAAAGTTGGAAAACCAAGTCCCTTTTTTTCTTCTACAAAATCTTTTTGACTTGATACCAAAACGTGCTATCCCTACTAGAGAAAATGAAGTTGGCCTTATCTCCTTAATAGATATTACAAGCATGATTCTTAAGGGGTTTGTGTTCGTTCGTAAATACAAGATCAATGATTTGGATCATAAAAAACCTAAGCACTTGCTTGATTTCTTAGGTTCTTACTTCTTTCCCTTGGTCTCTAATGATGATCCTACACAGAACAAGCAATATGTGATAAGAACAATTAGTGATAAAAAAGACAACAATTTGTTAAGGTTCATTCGCCTCCTCTTTACGGCTCATTGGCAAAAGAAGAATAATGATTTATTTGATGTCTCGACTTTGTTCTAGTCTTCAAATAAGAAGAAAACGACTAATGAAGAAAACAGTGAGCATCATTTCAGGTTGAGTCCTCCATCTATGACAGAGTTATGCGAGGCTGGTGTCATCGTCAAAGCAGTAAAAAACGAAGATACATGTTTCATGAACATAAGTTTCGAAAATGGGGTTTTGAAAATTCCATGTTTAGAAATTGACTGTACCTTTGAAACTGTGATACGAAATGTGATAGCATTTGATCAATACCCTGCAGGAAATAAGAATGCGTATGCAATCCACTATGTGTTATTTCTGGATGATTTGATAAACACGGAGCAAGACGCGCATTTACTTGCGAATGTTGGAGTAATAATCAACATTTTAGGTGGCAGTGATAAAGACATTACAGAAATGTTTAACAATTTATCTAAATATGTAACCTTCCCAGTTTGTTCCCACTTCGATGATATCAGCAAAGCTTTACGTAAGCATTGCAACAAAAGATGGAACAAGGCCATAGCTTCGTTGAAACACAACTACTTCAATACGCCATGGGCTATTATCTCCTTCCTTGCTGCGACTATTCTCATTTTTCTTACCATCCTTCAAACAATATTCTCCGCTATCTCTACATTTCCTAACTAA

mRNA sequence

AGAAACAATCAGTGAGATTGATCCAAAAGTTTGTGGTTATGTTGTGAGTTCTATTGACAAAATATTGGAAGAACGGTTTCGAGTTGATACAAAATCCTATATCCCTCAATTCATTTCCATCGGTCCTTTTCACCATGGCACTCGAAATGATTTGACAGCTAATGAACATTATAAGATTCAAGGTTTTAATAATTTTCTACATCGTATAAATATTAATTATAAGCAGGTTGAATCATTGAAGGAGCTTGTGAAAAAATGTCATGGTTGGATGAAAGATGCTTGGAATTGTTATGCAGAACCAATAGAGATGGAGGAGAAGACGTTTGTTTTAATGATGCTTGTGGATGCTTGTTTCATACTCGAGTTTTTTATACTACTAATTGATCCCCTTGACATTGTAGACGTCTCATTCTACCACGGAAAATTATTTGAAATACTTGTTGACTTGATAAAGTTGGAAAACCAAGTCCCTTTTTTTCTTCTACAAAATCTTTTTGACTTGATACCAAAACGTGCTATCCCTACTAGAGAAAATGAAGTTGGCCTTATCTCCTTAATAGATATTACAAGCATGATTCTTAAGGGGTTTGTGTTCGTTCGTAAATACAAGATCAATGATTTGGATCATAAAAAACCTAAGCACTTGCTTGATTTCTTAGGTTCTTACTTCTTTCCCTTGGTCTCTAATGATGATCCTACACAGAACAAGCAATATGTGATAAGAACAATTAGTGATAAAAAAGACAACAATTTGTTAAGGTTGAGTCCTCCATCTATGACAGAGTTATGCGAGGCTGGTGTCATCGTCAAAGCAGTAAAAAACGAAGATACATGTTTCATGAACATAAGTTTCGAAAATGGGGTTTTGAAAATTCCATGTTTAGAAATTGACTGTACCTTTGAAACTGTGATACGAAATGTGATAGCATTTGATCAATACCCTGCAGGAAATAAGAATGCGTATGCAATCCACTATGTGTTATTTCTGGATGATTTGATAAACACGGAGCAAGACGCGCATTTACTTGCGAATGTTGGAGTAATAATCAACATTTTAGGTGGCAGTGATAAAGACATTACAGAAATGTTTAACAATTTATCTAAATATGTAACCTTCCCAGTTTGTTCCCACTTCGATGATATCAGCAAAGCTTTACGTAAGCATTGCAACAAAAGATGGAACAAGGCCATAGCTTCGTTGAAACACAACTACTTCAATACGCCATGGGCTATTATCTCCTTCCTTGCTGCGACTATTCTCATTTTTCTTACCATCCTTCAAACAATATTCTCCGCTATCTCTACATTTCCTAACTAA

Coding sequence (CDS)

AGAAACAATCAGTGAGATTGATCCAAAAGTTTGTGGTTATGTTGTGAGTTCTATTGACAAAATATTGGAAGAACGGTTTCGAGTTGATACAAAATCCTATATCCCTCAATTCATTTCCATCGGTCCTTTTCACCATGGCACTCGAAATGATTTGACAGCTAATGAACATTATAAGATTCAAGGTTTTAATAATTTTCTACATCGTATAAATATTAATTATAAGCAGGTTGAATCATTGAAGGAGCTTGTGAAAAAATGTCATGGTTGGATGAAAGATGCTTGGAATTGTTATGCAGAACCAATAGAGATGGAGGAGAAGACGTTTGTTTTAATGATGCTTGTGGATGCTTGTTTCATACTCGAGTTTTTTATACTACTAATTGATCCCCTTGACATTGTAGACGTCTCATTCTACCACGGAAAATTATTTGAAATACTTGTTGACTTGATAAAGTTGGAAAACCAAGTCCCTTTTTTTCTTCTACAAAATCTTTTTGACTTGATACCAAAACGTGCTATCCCTACTAGAGAAAATGAAGTTGGCCTTATCTCCTTAATAGATATTACAAGCATGATTCTTAAGGGGTTTGTGTTCGTTCGTAAATACAAGATCAATGATTTGGATCATAAAAAACCTAAGCACTTGCTTGATTTCTTAGGTTCTTACTTCTTTCCCTTGGTCTCTAATGATGATCCTACACAGAACAAGCAATATGTGATAAGAACAATTAGTGATAAAAAAGACAACAATTTGTTAAGGTTGAGTCCTCCATCTATGACAGAGTTATGCGAGGCTGGTGTCATCGTCAAAGCAGTAAAAAACGAAGATACATGTTTCATGAACATAAGTTTCGAAAATGGGGTTTTGAAAATTCCATGTTTAGAAATTGACTGTACCTTTGAAACTGTGATACGAAATGTGATAGCATTTGATCAATACCCTGCAGGAAATAAGAATGCGTATGCAATCCACTATGTGTTATTTCTGGATGATTTGATAAACACGGAGCAAGACGCGCATTTACTTGCGAATGTTGGAGTAATAATCAACATTTTAGGTGGCAGTGATAAAGACATTACAGAAATGTTTAACAATTTATCTAAATATGTAACCTTCCCAGTTTGTTCCCACTTCGATGATATCAGCAAAGCTTTACGTAAGCATTGCAACAAAAGATGGAACAAGGCCATAGCTTCGTTGAAACACAACTACTTCAATACGCCATGGGCTATTATCTCCTTCCTTGCTGCGACTATTCTCATTTTTCTTACCATCCTTCAAACAATATTCTCCGCTATCTCTACATTTCCTAACTAA

Protein sequence

ETISEIDPKVCGYVVSSIDKILEERFRVDTKSYIPQFISIGPFHHGTRNDLTANEHYKIQGFNNFLHRININYKQVESLKELVKKCHGWMKDAWNCYAEPIEMEEKTFVLMMLVDACFILEFFILLIDPLDIVDVSFYHGKLFEILVDLIKLENQVPFFLLQNLFDLIPKRAIPTRENEVGLISLIDITSMILKGFVFVRKYKINDLDHKKPKHLLDFLGSYFFPLVSNDDPTQNKQYVIRTISDKKDNNLLRLSPPSMTELCEAGVIVKAVKNEDTCFMNISFENGVLKIPCLEIDCTFETVIRNVIAFDQYPAGNKNAYAIHYVLFLDDLINTEQDAHLLANVGVIINILGGSDKDITEMFNNLSKYVTFPVCSHFDDISKALRKHCNKRWNKAIASLKHNYFNTPWAIISFLAATILIFLTILQTIFSAISTFPN*
BLAST of Cucsa.162320.1 vs. Swiss-Prot
Match: Y3720_ARATH (UPF0481 protein At3g47200 OS=Arabidopsis thaliana GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 5.8e-34
Identity = 111/433 (25.64%), Postives = 212/433 (48.96%), Query Frame = 1

Query: 18  IDKILEERFRVDTKSYIPQFISIGPFHHGTRNDLTANEHYKIQGFNNFLHRI-------N 77
           I ++ E    ++ K+Y P+ +SIGP+H+G ++ L   + +K +    FL          N
Sbjct: 48  IFRVPESFVALNPKAYKPKVVSIGPYHYGEKH-LQMIQQHKPRLLQLFLDEAKKKDVEEN 107

Query: 78  INYKQVESLKELVKKCHGWMKDAWNCYAEPIEMEEKTFVLMMLVDACFILEFFILLIDPL 137
           +  K V  L++ ++K           Y+E ++      + MM++D CFIL  F+++   +
Sbjct: 108 VLVKAVVDLEDKIRKS----------YSEELKTGHD-LMFMMVLDGCFILMVFLIMSGNI 167

Query: 138 DIVDVSFYH--GKLFEILVDLIKLENQVPFFLLQNLFDLIPKRAIPTRENEVGLISLIDI 197
           ++ +   +     L  I  DL+ LENQVPFF+LQ L+ +  K  +    +++  I+    
Sbjct: 168 ELSEDPIFSIPWLLSSIQSDLLLLENQVPFFVLQTLY-VGSKIGV---SSDLNRIAFHFF 227

Query: 198 TSMILKGFVFVRKYKINDLDHKKPKHLLDFLGSYFFPLVSNDDPTQNKQYVIRTISDKKD 257
            + I K   +  K++     + K KHLLD +   F P  S  D   +    ++ + + K 
Sbjct: 228 KNPIDKEGSYWEKHR-----NYKAKHLLDLIRETFLPNTSESDKASSPHVQVQ-LHEGKS 287

Query: 258 NNLLRLSPPSMTELCEA------GVIVKAVKNEDTCFMNISFENGVLKIPCLEIDCTFET 317
            N+  +   ++  +  A      G+  +  ++++   +N+  +   L+IP L  D    +
Sbjct: 288 GNVPSVDSKAVPLILSAKRLRLQGIKFRLRRSKEDSILNVRLKKNKLQIPQLRFDGFISS 347

Query: 318 VIRNVIAFDQYPAGNKNAYAIHYVLFLDDLINTEQDAHLLANVGVIINILGGSDKDITEM 377
              N +AF+Q+   + N     Y++F+  L+N E+D   L N  +II    GS+ +++E 
Sbjct: 348 FFLNCVAFEQFYTDSSNEITT-YIVFMGCLLNNEEDVTFLRNDKLIIENHFGSNNEVSEF 407

Query: 378 FNNLSKYVTFPV-CSHFDDISKALRKHCNKRWNKAIASLKHNYFNTPWAIISFLAATILI 435
           F  +SK V F V  S+ +++ K + ++  K +N   A  +H +F +PW  +S  A   +I
Sbjct: 408 FKTISKDVVFEVDTSYLNNVFKGVNEYTKKWYNGLWAGFRHTHFESPWTFLSSCAVLFVI 457

BLAST of Cucsa.162320.1 vs. Swiss-Prot
Match: Y3264_ARATH (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 2.9e-17
Identity = 59/221 (26.70%), Postives = 105/221 (47.51%), Query Frame = 1

Query: 216 LDFLGSYFFPLVSNDDPTQNKQYVIRTISDKKDNNLLRLSPPSMTELCEAGVIVKAVKNE 275
           L     Y F    N+  T  +Q  +  +  +K   +  L+ PS+++L +AGV  K   + 
Sbjct: 306 LKLSADYLFTRQENE-ATTTRQESVSILDIEKPPLVEELTIPSVSDLHKAGVRFKPTAHG 365

Query: 276 DTCFMNISFENGVLKIPCLEIDCTFETVIRNVIAFDQYPAGNKNAYAIHYVLFLDDLINT 335
           +   +     +G   +P + +D   ETV+RN++A++         +   Y   ++ +I++
Sbjct: 366 NISTVTFDSNSGQFYLPVINLDINTETVLRNLVAYEATNTSGPLVFT-RYTELINGIIDS 425

Query: 336 EQDAHLLANVGVIINILGGSDKDITEMFNNLSKYVTFPVCSHFDDISKALRKHCNKRWNK 395
           E+D  LL   GV+++ L  SD++  EM+N +SK V        D   + + ++   RW  
Sbjct: 426 EEDVRLLREQGVLVSRL-KSDQEAAEMWNGMSKSVRLTKVGFLDKTIEDVNRYYTGRWKV 485

Query: 396 AIASLKHNYFNTPWAIISFLAATILIFLTILQTIFSAISTF 437
            I  L   Y    W I++FLAA +L+ L  LQ      S+F
Sbjct: 486 KIGRLVEVYVYGSWQILAFLAAVLLLMLVSLQLFSLVFSSF 523

BLAST of Cucsa.162320.1 vs. TrEMBL
Match: A0A0A0LPJ5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G380630 PE=4 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 1.9e-153
Identity = 300/395 (75.95%), Postives = 304/395 (76.96%), Query Frame = 1

Query: 103 MEEKTFVLMMLVDACFILEFFILLIDPL----------------DIVDVSFYHGKLFEIL 162
           MEEKTFVLMMLVDACFILEFFILLIDPL                DIVD SFY GK F+IL
Sbjct: 1   MEEKTFVLMMLVDACFILEFFILLIDPLGHGRNLFGVDHFFQIQDIVDFSFYQGKFFQIL 60

Query: 163 VDLIKLENQVPFFLLQNLFDLIPKRAIPTRENEVGLISLIDITSMILKGFVFVRKYKIND 222
           VDLIKLENQVPFFLLQNLFDLIPKRAIPT ENEVGLISLIDITSMILKGFVFVRKYKIND
Sbjct: 61  VDLIKLENQVPFFLLQNLFDLIPKRAIPTGENEVGLISLIDITSMILKGFVFVRKYKIND 120

Query: 223 LDHKKPKHLLDFLGSYFFPLVSNDDPTQNKQYVIRTISDKKDNNLLRLSPPSMT------ 282
           LDHKKPKHLLDFLGSYFFPL SNDDPTQNKQYVI TISDKKDNNLLR      T      
Sbjct: 121 LDHKKPKHLLDFLGSYFFPLASNDDPTQNKQYVITTISDKKDNNLLRFIRLLFTAHWQKK 180

Query: 283 --ELCEAGVIV-----KAVKNEDTC---------FMNISFENG----------------- 342
             +L     +      K   NE+            M    E G                 
Sbjct: 181 NNDLFNVSAMCYSSNKKTTTNEENSEHHFRLSPPSMTELCEAGVIVKAVKNEDTCFMNIS 240

Query: 343 ----VLKIPCLEIDCTFETVIRNVIAFDQYPAGNKNAYAIHYVLFLDDLINTEQDAHLLA 402
               VLKIPCLEIDCTFE VIRNVIAFDQYPAGNKNAYAIHYVLFLDDLINTEQDAHLLA
Sbjct: 241 FENGVLKIPCLEIDCTFEIVIRNVIAFDQYPAGNKNAYAIHYVLFLDDLINTEQDAHLLA 300

Query: 403 NVGVIINILGGSDKDITEMFNNLSKYVTFPVCSHFDDISKALRKHCNKRWNKAIASLKHN 439
           NVGVIIN LGGSDKDITEMFNNLSKYVTFPVCSHFDDISK LRKHCNKRWNKAIASLKHN
Sbjct: 301 NVGVIINTLGGSDKDITEMFNNLSKYVTFPVCSHFDDISKDLRKHCNKRWNKAIASLKHN 360

BLAST of Cucsa.162320.1 vs. TrEMBL
Match: A0A0A0LQ57_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G380610 PE=4 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 5.3e-135
Identity = 271/477 (56.81%), Postives = 336/477 (70.44%), Query Frame = 1

Query: 1   ETISE-IDPKVCGYVVSSIDKILE-----------------ERFRVDTKSYIPQFISIGP 60
           E +SE ID  V   VV SIDK+LE                 E   ++ K+Y+PQFISIGP
Sbjct: 7   EIMSENIDQNVRDNVVISIDKMLEGLPRVNPKCNTIYQVPKELREINDKAYVPQFISIGP 66

Query: 61  FHHGTRNDLTANEHYKIQGFNNFLHRININYKQVESLKE---------LVKKCHGWMKDA 120
           FH+ TR DL ANEHYK+QGF NFL RI+I    ++ L+E         LV+K H W+K+A
Sbjct: 67  FHYRTRKDLIANEHYKLQGFFNFLGRISIINSHIQLLEENQVNISSKVLVEKSHDWVKEA 126

Query: 121 WNCYAEPIEMEEKTFVLMMLVDACFILEFFILLIDP------LDIVDVS-FYHGKLFEIL 180
           WNCYAEPI+M+++ F++MMLVDACFI+EFF+L          L   ++S FY+G  +EIL
Sbjct: 127 WNCYAEPIKMKDEEFIIMMLVDACFIVEFFLLYYGSFHEDGKLFNAELSLFYYGVFYEIL 186

Query: 181 VDLIKLENQVPFFLLQNLFDLIPKRAIPTRENEVGL----ISLIDITSMILKGFVFVRKY 240
           +DLIKLENQVPFFLLQNLFDL+PK  +       G     ISLID+T M+LK F FVR+Y
Sbjct: 187 LDLIKLENQVPFFLLQNLFDLMPKDKVDISSIIGGYKDSPISLIDLTYMVLKEFGFVREY 246

Query: 241 KINDLDHKKPKHLLDFLGSYFFPLVSNDDPTQNKQYVIRTISDKKDNNLLRLSPPSMTEL 300
           KIN+L HK PKHLLDFL  YF P+  N+    N+++    IS +      RLSPP+ TEL
Sbjct: 247 KINNLYHKNPKHLLDFLSFYFLPVPPNN---WNRKFDHVKISKQ-----WRLSPPTTTEL 306

Query: 301 CEAGVIVKAVKNEDT-CFMNISFENGVLKIPCLEIDCTFETVIRNVIAFDQYPAGNKNAY 360
           CEAGV +K  K ++  CFMNISF+NGVL+IP + I+ TFE +IRNV+AF+ +PAGN+  Y
Sbjct: 307 CEAGVTIKVAKRKNNLCFMNISFKNGVLEIPPIVIEGTFEVLIRNVLAFEIFPAGNQKKY 366

Query: 361 AIHYVLFLDDLINTEQDAHLLANVGVIINILGGSDKDITEMFNNLSKYVTFPVCSHFDDI 420
           AI YV FLDDLI+TE+D  LL   GVIIN  GGSDK+++E+FN+L+K VT P+ S+FDD 
Sbjct: 367 AIQYVTFLDDLISTEKDLCLLVKAGVIINDTGGSDKEVSELFNSLTKLVTTPLPSYFDDT 426

Query: 421 SKALRKHCNKRWNKAIASLKHNYFNTPWAIISFLAATILIFLTILQTIFSAISTFPN 439
           SKALR HC+  WNKA ASLKH+YFNTPWAIISF AAT LI LTILQTIFSAIS FPN
Sbjct: 427 SKALRVHCDGSWNKAKASLKHSYFNTPWAIISFFAATFLIILTILQTIFSAISAFPN 475

BLAST of Cucsa.162320.1 vs. TrEMBL
Match: A0A0A0LSF4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G380060 PE=4 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 2.3e-98
Identity = 218/469 (46.48%), Postives = 300/469 (63.97%), Query Frame = 1

Query: 17  SIDKILEERFRVDTKSYIPQFISIGPFHHGT-RNDLTANEHYKIQGFNNFLHRIN-INYK 76
           SI ++ ++   ++ K+YIPQFISIGPFHH   +N+    + YK  G  NFL RIN    K
Sbjct: 55  SIYRVPQQLCDMNPKAYIPQFISIGPFHHSKCQNNSKGTQKYKHYGLFNFLRRINNTEIK 114

Query: 77  QVE------------SLKELVKKCHGWMKDAWNCYAEPIE-MEEKTFVLMMLVDACFILE 136
           + E            +LK LV+K H W++    C++EPI+ M++  FV+MML+DACFI+E
Sbjct: 115 EEEDVMTKSRSFQPKTLKVLVEKVHDWVEKTRYCFSEPIDYMDDHNFVIMMLMDACFIVE 174

Query: 137 FFILLID---------PL--DIVDVSFYHGKLFE-ILVDLIKLENQVPFFLLQNLFDLIP 196
           FFI + D         PL  D V++     ++ + I+ DLIKLENQVPFFLLQ++FD+IP
Sbjct: 175 FFIQMYDDQFDQDDQFPLISDKVNILSLFPEITDFIMFDLIKLENQVPFFLLQHVFDMIP 234

Query: 197 KRAIPTRENEVGLISLIDITSMILK-GFVFVRKYKINDLDH----KKPKHLLDFLGSYFF 256
           +  +         +S ID+T   L+ GF+F   YKI   D+    K  KHL+ FL  YF 
Sbjct: 235 RHNLH--------VSFIDLTYCALRIGFIF--NYKIYSFDYDFVKKDLKHLVHFLSLYFI 294

Query: 257 PLVSNDDPTQNKQ--------YVIRTIS-------DKKDNNLLRLSPPSMTELCEAGVIV 316
           P  + +D  QNK+        +  R +        DK++++   LSPPS+TEL E+GV V
Sbjct: 295 PPPTTNDMIQNKKKNNYSFLSFFSRLLCCELCQNPDKENSDQEFLSPPSITELNESGVTV 354

Query: 317 KAVKNEDTCFMNISFENGVLKIPCLEIDCTFETVIRNVIAFDQYPAGNKNAYAIHYVLFL 376
           K  KN ++  MNI+F NGVL+IP L I   FE + RN++AF+Q+ AGN   YA  Y++F+
Sbjct: 355 KKAKNVES-LMNITFTNGVLEIPPLNICDEFELLFRNLVAFEQFQAGNGKMYATQYIIFM 414

Query: 377 DDLINTEQDAHLLANVGVIINILGGSDKDITEMFNNLSKYVT-FPVCSHFDDISKALRKH 436
           D+LINT++D  LL N GVIIN +GGS K+++++FN L K+++  P  S F+DISK L KH
Sbjct: 415 DNLINTKKDVRLLVNSGVIINNIGGSYKEVSKLFNKLGKFISGSPTSSEFNDISKDLHKH 474

Query: 437 CNKRWNKAIASLKHNYFNTPWAIISFLAATILIFLTILQTIFSAISTFP 438
           CN+RWNKA ASLKHNYFNTPWAI+SF+AA+ LI LT+LQTIFS  S FP
Sbjct: 475 CNRRWNKAKASLKHNYFNTPWAIVSFIAASCLILLTLLQTIFSGFSAFP 512

BLAST of Cucsa.162320.1 vs. TrEMBL
Match: A0A0A0LLX1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G380590 PE=4 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 1.2e-94
Identity = 199/445 (44.72%), Postives = 284/445 (63.82%), Query Frame = 1

Query: 17  SIDKILEERFRVDTKSYIPQFISIGPFHHGTRNDLTANEHYKIQGFNNFLHRININYK-- 76
           SI ++ +++  ++ K+Y+PQ ISIGPF++    +L ANE YK Q   NFL R+   YK  
Sbjct: 55  SIYRVPKQQREMNPKAYVPQHISIGPFYYHADENLRANEQYKFQSVINFLRRV---YKIE 114

Query: 77  ------QVESLKELVKKCHGWMKDAWNCYAEPIEMEEKTFVLMMLVDACFILEFFILLID 136
                 Q  SL++LVKK   W+K+A NCYAE I+M ++ F+ MML+D CFI+EFFIL  +
Sbjct: 115 SLEQLLQTRSLEDLVKKAQSWVKEARNCYAESIDMNDEDFIKMMLMDGCFIVEFFILDYE 174

Query: 137 PLDIVD------------VSFYHGKLFEILVDLIKLENQVPFFLLQNLFDLIPKRAIPTR 196
              + D            +SFY  ++ +I  DLIKLENQ+PFF+LQ+L+DLIPK+    +
Sbjct: 175 EYKMPDESFFPKIENNVSMSFYKERIPDIDDDLIKLENQLPFFILQHLYDLIPKQDDNPK 234

Query: 197 ENEVGLISLIDITSMILKGFVFVRKYKINDLDHKKPKHLLDFLGSYFFPLVSNDDPTQNK 256
                     ++T   LK   ++  Y+ +D+   +PKH +DFL  YF P           
Sbjct: 235 -------CFKELTCKYLK-MGWLENYEPSDIQSIEPKHFIDFLSFYFVPHH--------- 294

Query: 257 QYVIRTISDKKDNNLLR---LSPPSMTELCEAGVIVKAVKNEDTCFMNISFENGVLKIPC 316
               R   DKK ++L     + PPS+TEL EAGV +K  +N   C MNI F+NG+L+IP 
Sbjct: 295 ----RCEHDKKSSDLEEWNVIIPPSITELFEAGVTIKKAENTK-CLMNIKFKNGILEIPP 354

Query: 317 LEIDCTFETVIRNVIAFDQYPAGNKNAYAIHYVLFLDDLINTEQDAHLLANVGVIINILG 376
           L ID  FE ++R+++AF+ +P   +N Y I Y+ F+D LI+TE D +LL    +IIN +G
Sbjct: 355 LHIDDYFEPMMRDLLAFEHFPIEVQNTYVIPYITFMDYLISTENDVNLLVKEKIIINDIG 414

Query: 377 GSDKDITEMFNNLSKYVTFPVCS-HFDDISKALRKHCNKRWNKAIASLKHNYFNTPWAII 436
           GSD++++++FNNL K+V+    + +F+DIS+ LRKHC++ WNKA A+LKHNY NTPWA I
Sbjct: 415 GSDREVSQLFNNLCKFVSSSFNNIYFNDISERLRKHCDRWWNKAKATLKHNYCNTPWAFI 474

Query: 437 SFLAATILIFLTILQTIFSAISTFP 438
           SFLAA IL+ LT+LQT+FSAIS FP
Sbjct: 475 SFLAALILLILTVLQTVFSAISAFP 474

BLAST of Cucsa.162320.1 vs. TrEMBL
Match: A0A0A0LM96_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G380080 PE=4 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 1.3e-93
Identity = 209/459 (45.53%), Postives = 288/459 (62.75%), Query Frame = 1

Query: 3   ISEIDPK--VCGYVVSSIDKILEER----------FRV-------DTKSYIPQFISIGPF 62
           IS +D +  +C  VV SI+K+L++           +RV       + K+Y PQ ISIGPF
Sbjct: 30  ISGVDQQQLICDNVVISIEKMLDQVPSAQEKQCSIYRVPKQLCEMNPKAYAPQLISIGPF 89

Query: 63  HHGTRNDLTANEHYKIQGFNNFLHRIN-INYKQVE---SLKELVKKCHGWMKDAWNCYAE 122
           ++    +L ANE YK+QGFNNFLHR+N ++ +Q E   SL +LVKK   W+K+A NCYAE
Sbjct: 90  YYHAHKNLIANEQYKLQGFNNFLHRVNKMSLEQQERTRSLNDLVKKAQSWVKEARNCYAE 149

Query: 123 PIEMEEKTFVLMMLVDACFILEFFILLIDPLDIVDVSFYHGKLFEILVDLIKLENQVPFF 182
            I M ++ F+ MMLVD                         ++ +I  DLIKLENQ+PFF
Sbjct: 150 SINMNDEDFIKMMLVDE------------------------RIPDIDDDLIKLENQLPFF 209

Query: 183 LLQNLFDLIPKRAIPTRENEVGLISLIDITSMILKGFVFVRKYKINDLDHKKPKHLLDFL 242
           +LQ+LFDLIPK      +N      L   T   L    ++  Y+ +D+   KPKH +DFL
Sbjct: 210 VLQHLFDLIPKH----NDNPNCFKQL---TYKYLN-MGWLENYEPSDILSIKPKHFIDFL 269

Query: 243 GSYFFPLVSNDDPTQNKQYVIRTISDKKDNNLLRLSPPSMTELCEAGVIVKAVKNEDTCF 302
             YF P    +   ++        SD K+ N++   PPS+TELCEAGV +K  +N   C 
Sbjct: 270 SFYFVPHHRCEHDQES--------SDMKEWNVI--IPPSITELCEAGVTIKKAENTK-CL 329

Query: 303 MNISFENGVLKIPCLEIDCTFETVIRNVIAFDQYPAGNKNAYAIHYVLFLDDLINTEQDA 362
           MNI FENG+L+IP L ID  FE ++RN++AF+ +P    N Y I YV F+D LI+TE+D 
Sbjct: 330 MNIRFENGILEIPPLHIDDYFEPMMRNLLAFEHFPVEVNNTYVIPYVTFMDYLISTEKDV 389

Query: 363 HLLANVGVIINILGGSDKDITEMFNNLSKYV-TFPVCSHFDDISKALRKHCNKRWNKAIA 422
           +LL    +IIN +GGSD++++++FNNL K+V + P  ++F++IS+ LR+HC++ WNKA A
Sbjct: 390 NLLVKEKIIINDIGGSDREVSQLFNNLCKFVSSSPNDNYFNNISEGLREHCDRWWNKAKA 445

Query: 423 SLKHNYFNTPWAIISFLAATILIFLTILQTIFSAISTFP 438
           SLKHNYFNTPWA ISF AAT+L+ LTILQT+FSAIS FP
Sbjct: 450 SLKHNYFNTPWAAISFSAATVLLVLTILQTVFSAISAFP 445

BLAST of Cucsa.162320.1 vs. TAIR10
Match: AT4G31980.1 (AT4G31980.1 unknown protein)

HSP 1 Score: 194.9 bits (494), Expect = 1.0e-49
Identity = 134/441 (30.39%), Postives = 229/441 (51.93%), Query Frame = 1

Query: 3   ISEIDPKVCGYVVSSIDKILEERFRVDTKSYIPQFISIGPFHHGTRNDLTANEHYKIQGF 62
           +S +  K C Y      K+  +  R++  +Y P+ +S GP H G + +L A E  K +  
Sbjct: 286 LSSLSTKCCIY------KVPNKLRRLNPDAYTPRLVSFGPLHRG-KEELQAMEDQKYRYL 345

Query: 63  NNFLHRININYKQVESLKELVKKCHGWMKDAWNCYAEPIEMEEKTFVLMMLVDACFILEF 122
            +F+ R N       SL++LV+    W ++A +CYAE +++    FV M++VD  F++E 
Sbjct: 346 LSFIPRTN------SSLEDLVRLARTWEQNARSCYAEDVKLHSDEFVEMLVVDGSFLVEL 405

Query: 123 FILLIDPLDIVDVSFYHGK---LFEILVDLIKLENQVPFFLLQNLFDLIPKRAIPTRENE 182
            +    P    +     G    + ++  D+I +ENQ+PFF+++ +F L+          +
Sbjct: 406 LLRSHYPRLRGENDRIFGNSMMITDVCRDMILIENQLPFFVVKEIFLLL------LNYYQ 465

Query: 183 VGLISLIDITSMILKGFVFVRKYKINDLDHKK----PKHLLDFLGSYFFPLVSNDDPTQN 242
            G  S+I +             Y ++ +D +K    P+H +D L S + P          
Sbjct: 466 QGTPSIIQLAQRHFS-------YFLSRIDDEKFITEPEHFVDLLRSCYLP---------- 525

Query: 243 KQYVIRT--ISDKKDNNLLRLSPPSMTELCEAGVIVKAVKNEDTCFMNISFENGVLKIPC 302
            Q+ I+    + K DN       P  TEL  AGV  K  +   +C ++ISF +GVLKIP 
Sbjct: 526 -QFPIKLEYTTVKVDN------APEATELHTAGVRFKPAETS-SCLLDISFADGVLKIPT 585

Query: 303 LEIDCTFETVIRNVIAFDQYPAGNKNAYAIHYVLFLDDLINTEQDAHLLANVGVIINILG 362
           + +D   E++ +N+I F+Q    NKN   + Y++ L   I +  DA LL + G+I+N LG
Sbjct: 586 IVVDDLTESLYKNIIGFEQCRCSNKN--FLDYIMLLGCFIKSPTDADLLIHSGIIVNYLG 645

Query: 363 GSDKDITEMFNNLSKYVTFPVCSHFDDISKALRKHCNKRWNKAIASLKHNYFNTPWAIIS 422
            S  D++ +FN++SK V +    +F  +S+ L+ +CN  WN+  A L+ +YF+ PWA+ S
Sbjct: 646 NS-VDVSNLFNSISKEVIYDRRFYFSMLSENLQAYCNTPWNRWKAILRRDYFHNPWAVAS 679

Query: 423 FLAATILIFLTILQTIFSAIS 435
             AA +L+ LT +Q++ S ++
Sbjct: 706 VFAALLLLLLTFIQSVCSILA 679

BLAST of Cucsa.162320.1 vs. TAIR10
Match: AT3G50170.1 (AT3G50170.1 Plant protein of unknown function (DUF247))

HSP 1 Score: 184.9 bits (468), Expect = 1.1e-46
Identity = 134/438 (30.59%), Postives = 221/438 (50.46%), Query Frame = 1

Query: 29  DTKSYIPQFISIGPFHHGTRNDLTANEHYKIQGFNNFLHRININYKQ-VESLKELVKKCH 88
           D KSY PQ +S+GP+HHG +  L   E +K +  N  L R+    +    +++EL +K  
Sbjct: 126 DKKSYFPQTVSLGPYHHGKKR-LRPMERHKWRALNKVLKRLKQRIEMYTNAMRELEEKAR 185

Query: 89  GWMKDAWNCYAEPIEMEEKTFVLMMLVDACFILEFFILLIDPLDIVD------VSFYHGK 148
                   CY  PI +    F  M+++D CF+LE F   ++    +       V    G 
Sbjct: 186 A-------CYEGPISLSRNEFTEMLVLDGCFVLELFRGTVEGFTEIGYARNDPVFAMRGL 245

Query: 149 LFEILVDLIKLENQVPFFLLQNLFDLIPKRAIPTRENEVGLISLIDIT---SMILKGFVF 208
           +  I  D+I LENQ+P F+L  L +L     + T +N+ G+++ + +     ++  G   
Sbjct: 246 MHSIQRDMIMLENQLPLFVLDRLLEL----QLGT-QNQTGIVAHVAVKFFDPLMPTGEAL 305

Query: 209 VR----------KYKINDLDHKKPKHLLDFLGSYFFPLVSNDDPTQNKQYVIRTIS---- 268
            +          +  ++ L  K   H LD     F   +    PT N + +++ ++    
Sbjct: 306 TKPDQSKLMNWLEKSLDTLGDKGELHCLDV----FRRSLLQSSPTPNTRSLLKRLTRNTR 365

Query: 269 --DKKDNNLLRLSPPSMTELCEAGVIVKAVKNEDTCFMNISFENGVLKIPCLEIDCTFET 328
             DK+   L+      +TEL EAGV  K  K +   F +I F+NG L+IP L I    ++
Sbjct: 366 VVDKRQQQLVHC----VTELREAGV--KFRKRKTDRFWDIEFKNGYLEIPKLLIHDGTKS 425

Query: 329 VIRNVIAFDQYPAGNKNAYAIHYVLFLDDLINTEQDAHLLANVGVIINILGGSDKDITEM 388
           +  N+IAF+Q    + N +   Y++F+D+LIN+ +D   L   G+I + L GSD ++ ++
Sbjct: 426 LFSNLIAFEQCHIESSN-HITSYIIFMDNLINSSEDVSYLHYCGIIEHWL-GSDSEVADL 485

Query: 389 FNNLSKYVTF-PVCSHFDDISKALRKHCNKRWNKAIASLKHNYFNTPWAIISFLAATILI 439
           FN L + V F P  SH   +S  + ++ N++WN   A+L H YFN PWA  SF AA IL+
Sbjct: 486 FNRLCQEVVFDPKDSHLSRLSGDVNRYYNRKWNVLKATLTHKYFNNPWAYFSFSAAVILL 538

BLAST of Cucsa.162320.1 vs. TAIR10
Match: AT3G50150.1 (AT3G50150.1 Plant protein of unknown function (DUF247))

HSP 1 Score: 177.9 bits (450), Expect = 1.3e-44
Identity = 129/424 (30.42%), Postives = 212/424 (50.00%), Query Frame = 1

Query: 29  DTKSYIPQFISIGPFHHGTRNDLTANEHYKIQGFNNFLHRININYKQ-VESLKELVKKCH 88
           D KSY+PQ +SIGP+HHG +  L   E +K +  N  + R   N +  ++++KEL ++  
Sbjct: 103 DKKSYLPQTVSIGPYHHG-KVHLRPMERHKWRAVNMIMARTKHNIEMYIDAMKELEEEAR 162

Query: 89  GWMKDAWNCYAEPIEMEEKT-FVLMMLVDACFILEFFILLIDPLDIVD------VSFYHG 148
                   CY  PI+M+    F  M+++D CF+LE F   I     +       V    G
Sbjct: 163 A-------CYQGPIDMKNSNEFTEMLVLDGCFVLELFKGTIQGFQKIGYARNDPVFAKRG 222

Query: 149 KLFEILVDLIKLENQVPFFLLQNLFDLIPKRAIPTRENEVGLISLIDITSMILKGFVFVR 208
            +  I  D+I LENQ+P F+L  L  L  +   P +   V  +++    +++    V  +
Sbjct: 223 LMHSIQRDMIMLENQLPLFVLDRLLGL--QTGTPNQTGIVAEVAVRFFKTLMPTSEVLTK 282

Query: 209 KYKINDLDHKKPK-------HLLDFLGSYFFPLVSNDDPTQNKQYVIRTISDKKDNNLLR 268
             +  D   K  +       H LD        + S++   Q   Y   ++ +K+   +  
Sbjct: 283 SERSLDSQEKSDELGDNGGLHCLDVFHRSL--IQSSETTNQGTPYEDMSMVEKQQQLI-- 342

Query: 269 LSPPSMTELCEAGVIVKAVKNEDTCFMNISFENGVLKIPCLEIDCTFETVIRNVIAFDQY 328
                +TEL  AGV    ++ E     +I F+NG LKIP L I    +++  N+IAF+Q 
Sbjct: 343 ---HCVTELRGAGV--NFMRKETGQLWDIEFKNGYLKIPKLLIHDGTKSLFSNLIAFEQC 402

Query: 329 PAGNKNAYAIHYVLFLDDLINTEQDAHLLANVGVIINILGGSDKDITEMFNNLSKYVTF- 388
              + N     Y++F+D+LIN+ QD   L + G+I + L GSD ++ ++FN L K V F 
Sbjct: 403 HTQSSNNIT-SYIIFMDNLINSSQDVSYLHHDGIIEHWL-GSDSEVADLFNRLCKEVIFD 462

Query: 389 PVCSHFDDISKALRKHCNKRWNKAIASLKHNYFNTPWAIISFLAATILIFLTILQTIFSA 437
           P   +   +S+ + ++ +++WN   A+L+  YFN PWA  SF AA IL+FLT  Q+ F+ 
Sbjct: 463 PKDGYLSQLSREVNRYYSRKWNSLKATLRQKYFNNPWAYFSFSAAVILLFLTFFQSFFAV 505

BLAST of Cucsa.162320.1 vs. TAIR10
Match: AT3G50130.1 (AT3G50130.1 Plant protein of unknown function (DUF247))

HSP 1 Score: 177.6 bits (449), Expect = 1.7e-44
Identity = 141/454 (31.06%), Postives = 231/454 (50.88%), Query Frame = 1

Query: 9   KVCGYVVSSIDKILEERFRVDTKSYIPQFISIGPFHHGTRNDLTANEHYKIQGFNNFLHR 68
           K+C Y    + + L+E    + KSY PQ +S+GPFHHG ++ L  + H K +  N  + R
Sbjct: 138 KLCIY---RVPQYLQEN---NKKSYFPQTVSLGPFHHGNKHLLPMDRH-KWRAVNMVMAR 197

Query: 69  ININYKQ-VESLKELVKKCHGWMKDAWNCYAEPIEMEEKTFVLMMLVDACFILEFFI--- 128
              + +  ++++KEL  +          CY  PI++    F  M+++D CF+LE F    
Sbjct: 198 TKHDIEMYIDAMKELEDRARA-------CYEGPIDLSSNKFSEMLVLDGCFVLELFRGAD 257

Query: 129 -----LLIDPLDIVDVSFYHGKLFEILVDLIKLENQVPFFLLQNLFDL-IPKRAIPTREN 188
                L  D  D   V    G +  I  D++ LENQ+P F+L  L ++ + KR      +
Sbjct: 258 EGFSELGYDRND--PVFAMRGSMHSIQRDMVMLENQLPLFVLNRLLEIQLGKR------H 317

Query: 189 EVGLIS---------LIDITSMILKGFVFVRKYK----INDLDHKKPKHLLDFLGSYFFP 248
           + GL+S         L+     + K    + + K    I D D K   H LD        
Sbjct: 318 QTGLVSRLAVRFFDPLMPTDEPLTKTDDSLEQDKFFNPIADKD-KGELHCLDVFRRNLLR 377

Query: 249 LVSNDDPTQNK-QYVIRT-ISDKKDNNLLRLSPPSMTELCEAGVIVKAVKNEDTCFMNIS 308
             SN +P  ++ ++  RT ++DK+   L+      +TEL EAG+  +  K +   F +I 
Sbjct: 378 PCSNPEPRLSRMRWSWRTRVADKRQQQLIHC----VTELREAGIKFRTRKTDR--FWDIR 437

Query: 309 FENGVLKIPCLEIDCTFETVIRNVIAFDQYPAGNKNAYAIHYVLFLDDLINTEQDAHLLA 368
           F+NG L+IP L I    +++  N+IAF+Q    + N     Y++F+D+LI++ +D   L 
Sbjct: 438 FKNGYLEIPKLLIHDGTKSLFSNLIAFEQCHIDSSNDIT-SYIIFMDNLIDSSEDVRYLH 497

Query: 369 NVGVIINILGGSDKDITEMFNNLSKYVTF-PVCSHFDDISKALRKHCNKRWNKAIASLKH 428
             G+I + LG +D ++ ++FN L + V F P  S+   +S  + ++ +++WN   A LKH
Sbjct: 498 YCGIIEHWLG-NDYEVADLFNRLCQEVAFDPQNSYLSQLSNKVDRNYSRKWNVLKAILKH 557

Query: 429 NYFNTPWAIISFLAATILIFLTILQTIFSAISTF 437
            YFN PWA  SF AA +L+ LT+ Q+ F+A   F
Sbjct: 558 KYFNNPWAYFSFFAALVLLVLTLFQSFFTAYPYF 560

BLAST of Cucsa.162320.1 vs. TAIR10
Match: AT3G50120.1 (AT3G50120.1 Plant protein of unknown function (DUF247))

HSP 1 Score: 174.9 bits (442), Expect = 1.1e-43
Identity = 126/433 (29.10%), Postives = 218/433 (50.35%), Query Frame = 1

Query: 29  DTKSYIPQFISIGPFHHGTRNDLTANEHYKIQGFNNFLHRININYKQ-VESLKELVKKCH 88
           D KSY PQ +S+GP+HHG +  L + + +K +  N  L R N   K  +++++EL +K  
Sbjct: 116 DNKSYFPQTVSLGPYHHGKKR-LRSMDRHKWRAVNRVLKRTNQGIKMYIDAMRELEEKAR 175

Query: 89  GWMKDAWNCYAEPIEMEEKTFVLMMLVDACFILEFFILLIDPLDIVD------VSFYHGK 148
                   CY  P+ +    F+ M+++D CF+LE F   ++    +       V    G 
Sbjct: 176 A-------CYEGPLSLSSNEFIEMLVLDGCFVLELFRGAVEGFTELGYARNDPVFAMRGS 235

Query: 149 LFEILVDLIKLENQVPFFLLQNLFDLIPKRAIPTRENEVGLISLIDITSM--ILKGFVFV 208
           +  I  D++ LENQ+P F+L  L +L     + TR N+ GL++ + I     ++     +
Sbjct: 236 MHSIQRDMVMLENQLPLFVLNRLLEL----QLGTR-NQTGLVAQLAIRFFDPLMPTDEPL 295

Query: 209 RKYKINDLDHKKPK-------------HLLDFLGSYFFPLVSNDDPTQNKQYVIRT--IS 268
            K   + L++   +             H LD             +P   ++   R   ++
Sbjct: 296 TKSGQSKLENSLARDKSFDPFADMGELHCLDVFRRSLLRSSPKPEPRLTRKRWSRNTRVA 355

Query: 269 DKKDNNLLRLSPPSMTELCEAGVIVKAVKNEDTCFMNISFENGVLKIPCLEIDCTFETVI 328
           DK+   L+      +TEL EAG+  +  K +   F ++ F+NG L+IP L I    +++ 
Sbjct: 356 DKRRQQLIHC----VTELKEAGIKFRRRKTDR--FWDMQFKNGYLEIPRLLIHDGTKSLF 415

Query: 329 RNVIAFDQYPAGNKNAYAIHYVLFLDDLINTEQDAHLLANVGVIINILGGSDKDITEMFN 388
            N+IAF+Q    + N     Y++F+D+LI++ +D   L   G+I + LG SD ++ ++FN
Sbjct: 416 LNLIAFEQCHIDSSNDIT-SYIIFMDNLIDSHEDVSYLHYCGIIEHWLG-SDSEVADLFN 475

Query: 389 NLSKYVTFPV-CSHFDDISKALRKHCNKRWNKAIASLKHNYFNTPWAIISFLAATILIFL 437
            L + V F    S+   +S  + ++ + +WN   A+LKH YFN PWAI+SF AA IL+ L
Sbjct: 476 RLCQEVVFDTEDSYLSRLSIEVNRYYDHKWNAWRATLKHKYFNNPWAIVSFCAAVILLVL 527

BLAST of Cucsa.162320.1 vs. NCBI nr
Match: gi|659088828|ref|XP_008445182.1| (PREDICTED: UPF0481 protein At3g47200-like [Cucumis melo])

HSP 1 Score: 595.1 bits (1533), Expect = 9.8e-167
Identity = 322/513 (62.77%), Postives = 352/513 (68.62%), Query Frame = 1

Query: 1   ETISEIDPKVCGYVVSSIDKIL-----------------EERFRVDTKSYIPQFISIGPF 60
           ETISEI+ KVC  VV SIDKIL                 +E   ++ K+Y PQFISIGPF
Sbjct: 22  ETISEIE-KVCDNVVISIDKILGGLPRINPKCHIIYQVSKELREMNDKAYAPQFISIGPF 81

Query: 61  HHGTRNDLTANEHYKIQGFNNFLHRININYKQVESLKELVKKCHGWMKDAWNCYAEPIEM 120
           HH TRNDL ANEHYK+QGFNNFLHRIN NY+Q+ES KE VKKCHGW+K+AWNCYAEPI M
Sbjct: 82  HHRTRNDLIANEHYKLQGFNNFLHRIN-NYEQIESSKEFVKKCHGWVKEAWNCYAEPINM 141

Query: 121 EEKTFVLMMLVDACFILEFFILLIDP----------------LDIVDVSFYHGKLFEILV 180
            E+ FVLMMLVDACFILEFFILLID                  D+VD SFY G  FEIL+
Sbjct: 142 NEEEFVLMMLVDACFILEFFILLIDDHYGGDYLFEADQIFQIQDMVDFSFYRGVFFEILI 201

Query: 181 DLIKLENQVPFFLLQNLFDLIPKRAIPTRENEVGLISLIDITSMILKGFVFVRKYKINDL 240
           DLIKLENQVPFFLLQNLFDL+PK  +P         SLIDITS IL  F FV KYKINDL
Sbjct: 202 DLIKLENQVPFFLLQNLFDLMPKHDVPM------FPSLIDITSEILTWFGFVGKYKINDL 261

Query: 241 DHKKPKHLLDFL-----------GSYFFPLVSNDDPTQNKQYVIR--------------- 300
            HKKPKHLLDFL               F      +  QN   ++R               
Sbjct: 262 YHKKPKHLLDFLSFYFFPLLPNDDHIRFKQNERKNSDQNNNNLLRFFRPLFPAHWLKKNN 321

Query: 301 ----------------TISDKKDNNLLRLSPPSMTELCEAGVIVKAVKNEDTCFMNISFE 360
                              +K   N  RLSPPS+TELCEAGV +KA K ED CFMNI F+
Sbjct: 322 DSFGVPSLCCFSNKEAETREKDSENYFRLSPPSITELCEAGVTIKAAKREDLCFMNIGFK 381

Query: 361 NGVLKIPCLEIDCTFETVIRNVIAFDQYPAGNKNAYAIHYVLFLDDLINTEQDAHLLANV 420
           NGVL+IPC++IDCTFE VIRNVIAFDQYPAGN+  YAIHYVLFLDDLINTEQDAHLL   
Sbjct: 382 NGVLEIPCIDIDCTFEVVIRNVIAFDQYPAGNEKMYAIHYVLFLDDLINTEQDAHLLTKA 441

Query: 421 GVIINILGGSDKDITEMFNNLSKYVTFPVCSHFDDISKALRKHCNKRWNKAIASLKHNYF 439
           G+IIN  GGSDKDITEMFN  SK+VTFP+CSHFDDI+KALR HCN RWN A ASLKHNYF
Sbjct: 442 GIIINTFGGSDKDITEMFNRFSKFVTFPICSHFDDINKALRMHCNGRWNMAKASLKHNYF 501

BLAST of Cucsa.162320.1 vs. NCBI nr
Match: gi|778674385|ref|XP_011650198.1| (PREDICTED: UPF0481 protein At3g47200-like [Cucumis sativus])

HSP 1 Score: 580.5 bits (1495), Expect = 2.5e-162
Identity = 313/408 (76.72%), Postives = 317/408 (77.70%), Query Frame = 1

Query: 90  MKDAWNCYAEPIEMEEKTFVLMMLVDACFILEFFILLIDPL----------------DIV 149
           MKDAWNCYAEPIEMEEKTFVLMMLVDACFILEFFILLIDPL                DIV
Sbjct: 1   MKDAWNCYAEPIEMEEKTFVLMMLVDACFILEFFILLIDPLGHGRNLFGVDHFFQIQDIV 60

Query: 150 DVSFYHGKLFEILVDLIKLENQVPFFLLQNLFDLIPKRAIPTRENEVGLISLIDITSMIL 209
           D SFY GK F+ILVDLIKLENQVPFFLLQNLFDLIPKRAIPT ENEVGLISLIDITSMIL
Sbjct: 61  DFSFYQGKFFQILVDLIKLENQVPFFLLQNLFDLIPKRAIPTGENEVGLISLIDITSMIL 120

Query: 210 KGFVFVRKYKINDLDHKKPKHLLDFLGSYFFPLVSNDDPTQNKQYVIRTISDKKDNNLLR 269
           KGFVFVRKYKINDLDHKKPKHLLDFLGSYFFPL SNDDPTQNKQYVI TISDKKDNNLLR
Sbjct: 121 KGFVFVRKYKINDLDHKKPKHLLDFLGSYFFPLASNDDPTQNKQYVITTISDKKDNNLLR 180

Query: 270 LSPPSMT--------ELCEAGVIV-----KAVKNEDTC---------FMNISFENG---- 329
                 T        +L     +      K   NE+            M    E G    
Sbjct: 181 FIRLLFTAHWQKKNNDLFNVSAMCYSSNKKTTTNEENSEHHFRLSPPSMTELCEAGVIVK 240

Query: 330 -----------------VLKIPCLEIDCTFETVIRNVIAFDQYPAGNKNAYAIHYVLFLD 389
                            VLKIPCLEIDCTFE VIRNVIAFDQYPAGNKNAYAIHYVLFLD
Sbjct: 241 AVKNEDTCFMNISFENGVLKIPCLEIDCTFEIVIRNVIAFDQYPAGNKNAYAIHYVLFLD 300

Query: 390 DLINTEQDAHLLANVGVIINILGGSDKDITEMFNNLSKYVTFPVCSHFDDISKALRKHCN 439
           DLINTEQDAHLLANVGVIIN LGGSDKDITEMFNNLSKYVTFPVCSHFDDISK LRKHCN
Sbjct: 301 DLINTEQDAHLLANVGVIINTLGGSDKDITEMFNNLSKYVTFPVCSHFDDISKDLRKHCN 360

BLAST of Cucsa.162320.1 vs. NCBI nr
Match: gi|700207815|gb|KGN62934.1| (hypothetical protein Csa_2G380630 [Cucumis sativus])

HSP 1 Score: 550.4 bits (1417), Expect = 2.8e-153
Identity = 300/395 (75.95%), Postives = 304/395 (76.96%), Query Frame = 1

Query: 103 MEEKTFVLMMLVDACFILEFFILLIDPL----------------DIVDVSFYHGKLFEIL 162
           MEEKTFVLMMLVDACFILEFFILLIDPL                DIVD SFY GK F+IL
Sbjct: 1   MEEKTFVLMMLVDACFILEFFILLIDPLGHGRNLFGVDHFFQIQDIVDFSFYQGKFFQIL 60

Query: 163 VDLIKLENQVPFFLLQNLFDLIPKRAIPTRENEVGLISLIDITSMILKGFVFVRKYKIND 222
           VDLIKLENQVPFFLLQNLFDLIPKRAIPT ENEVGLISLIDITSMILKGFVFVRKYKIND
Sbjct: 61  VDLIKLENQVPFFLLQNLFDLIPKRAIPTGENEVGLISLIDITSMILKGFVFVRKYKIND 120

Query: 223 LDHKKPKHLLDFLGSYFFPLVSNDDPTQNKQYVIRTISDKKDNNLLRLSPPSMT------ 282
           LDHKKPKHLLDFLGSYFFPL SNDDPTQNKQYVI TISDKKDNNLLR      T      
Sbjct: 121 LDHKKPKHLLDFLGSYFFPLASNDDPTQNKQYVITTISDKKDNNLLRFIRLLFTAHWQKK 180

Query: 283 --ELCEAGVIV-----KAVKNEDTC---------FMNISFENG----------------- 342
             +L     +      K   NE+            M    E G                 
Sbjct: 181 NNDLFNVSAMCYSSNKKTTTNEENSEHHFRLSPPSMTELCEAGVIVKAVKNEDTCFMNIS 240

Query: 343 ----VLKIPCLEIDCTFETVIRNVIAFDQYPAGNKNAYAIHYVLFLDDLINTEQDAHLLA 402
               VLKIPCLEIDCTFE VIRNVIAFDQYPAGNKNAYAIHYVLFLDDLINTEQDAHLLA
Sbjct: 241 FENGVLKIPCLEIDCTFEIVIRNVIAFDQYPAGNKNAYAIHYVLFLDDLINTEQDAHLLA 300

Query: 403 NVGVIINILGGSDKDITEMFNNLSKYVTFPVCSHFDDISKALRKHCNKRWNKAIASLKHN 439
           NVGVIIN LGGSDKDITEMFNNLSKYVTFPVCSHFDDISK LRKHCNKRWNKAIASLKHN
Sbjct: 301 NVGVIINTLGGSDKDITEMFNNLSKYVTFPVCSHFDDISKDLRKHCNKRWNKAIASLKHN 360

BLAST of Cucsa.162320.1 vs. NCBI nr
Match: gi|700207813|gb|KGN62932.1| (hypothetical protein Csa_2G380610 [Cucumis sativus])

HSP 1 Score: 489.2 bits (1258), Expect = 7.6e-135
Identity = 271/477 (56.81%), Postives = 336/477 (70.44%), Query Frame = 1

Query: 1   ETISE-IDPKVCGYVVSSIDKILE-----------------ERFRVDTKSYIPQFISIGP 60
           E +SE ID  V   VV SIDK+LE                 E   ++ K+Y+PQFISIGP
Sbjct: 7   EIMSENIDQNVRDNVVISIDKMLEGLPRVNPKCNTIYQVPKELREINDKAYVPQFISIGP 66

Query: 61  FHHGTRNDLTANEHYKIQGFNNFLHRININYKQVESLKE---------LVKKCHGWMKDA 120
           FH+ TR DL ANEHYK+QGF NFL RI+I    ++ L+E         LV+K H W+K+A
Sbjct: 67  FHYRTRKDLIANEHYKLQGFFNFLGRISIINSHIQLLEENQVNISSKVLVEKSHDWVKEA 126

Query: 121 WNCYAEPIEMEEKTFVLMMLVDACFILEFFILLIDP------LDIVDVS-FYHGKLFEIL 180
           WNCYAEPI+M+++ F++MMLVDACFI+EFF+L          L   ++S FY+G  +EIL
Sbjct: 127 WNCYAEPIKMKDEEFIIMMLVDACFIVEFFLLYYGSFHEDGKLFNAELSLFYYGVFYEIL 186

Query: 181 VDLIKLENQVPFFLLQNLFDLIPKRAIPTRENEVGL----ISLIDITSMILKGFVFVRKY 240
           +DLIKLENQVPFFLLQNLFDL+PK  +       G     ISLID+T M+LK F FVR+Y
Sbjct: 187 LDLIKLENQVPFFLLQNLFDLMPKDKVDISSIIGGYKDSPISLIDLTYMVLKEFGFVREY 246

Query: 241 KINDLDHKKPKHLLDFLGSYFFPLVSNDDPTQNKQYVIRTISDKKDNNLLRLSPPSMTEL 300
           KIN+L HK PKHLLDFL  YF P+  N+    N+++    IS +      RLSPP+ TEL
Sbjct: 247 KINNLYHKNPKHLLDFLSFYFLPVPPNN---WNRKFDHVKISKQ-----WRLSPPTTTEL 306

Query: 301 CEAGVIVKAVKNEDT-CFMNISFENGVLKIPCLEIDCTFETVIRNVIAFDQYPAGNKNAY 360
           CEAGV +K  K ++  CFMNISF+NGVL+IP + I+ TFE +IRNV+AF+ +PAGN+  Y
Sbjct: 307 CEAGVTIKVAKRKNNLCFMNISFKNGVLEIPPIVIEGTFEVLIRNVLAFEIFPAGNQKKY 366

Query: 361 AIHYVLFLDDLINTEQDAHLLANVGVIINILGGSDKDITEMFNNLSKYVTFPVCSHFDDI 420
           AI YV FLDDLI+TE+D  LL   GVIIN  GGSDK+++E+FN+L+K VT P+ S+FDD 
Sbjct: 367 AIQYVTFLDDLISTEKDLCLLVKAGVIINDTGGSDKEVSELFNSLTKLVTTPLPSYFDDT 426

Query: 421 SKALRKHCNKRWNKAIASLKHNYFNTPWAIISFLAATILIFLTILQTIFSAISTFPN 439
           SKALR HC+  WNKA ASLKH+YFNTPWAIISF AAT LI LTILQTIFSAIS FPN
Sbjct: 427 SKALRVHCDGSWNKAKASLKHSYFNTPWAIISFFAATFLIILTILQTIFSAISAFPN 475

BLAST of Cucsa.162320.1 vs. NCBI nr
Match: gi|778674382|ref|XP_004138863.2| (PREDICTED: UPF0481 protein At3g47200, partial [Cucumis sativus])

HSP 1 Score: 423.7 bits (1088), Expect = 3.9e-115
Identity = 228/393 (58.02%), Postives = 286/393 (72.77%), Query Frame = 1

Query: 58  KIQGFNNFLHRININYKQVESLKELVKKCHGWMKDAWNCYAEPIEMEEKTFVLMMLVDAC 117
           +I   N+ +  +  N   + S K LV+K H W+K+AWNCYAEPI+M+++ F++MMLVDAC
Sbjct: 140 RISIINSHIQLLEENQVNISS-KVLVEKSHDWVKEAWNCYAEPIKMKDEEFIIMMLVDAC 199

Query: 118 FILEFFILLIDP------LDIVDVS-FYHGKLFEILVDLIKLENQVPFFLLQNLFDLIPK 177
           FI+EFF+L          L   ++S FY+G  +EIL+DLIKLENQVPFFLLQNLFDL+PK
Sbjct: 200 FIVEFFLLYYGSFHEDGKLFNAELSLFYYGVFYEILLDLIKLENQVPFFLLQNLFDLMPK 259

Query: 178 RAIPTRENEVGL----ISLIDITSMILKGFVFVRKYKINDLDHKKPKHLLDFLGSYFFPL 237
             +       G     ISLID+T M+LK F FVR+YKIN+L HK PKHLLDFL  YF P+
Sbjct: 260 DKVDISSIIGGYKDSPISLIDLTYMVLKEFGFVREYKINNLYHKNPKHLLDFLSFYFLPV 319

Query: 238 VSNDDPTQNKQYVIRTISDKKDNNLLRLSPPSMTELCEAGVIVKAVKNEDT-CFMNISFE 297
             N+    N+++    IS +      RLSPP+ TELCEAGV +K  K ++  CFMNISF+
Sbjct: 320 PPNN---WNRKFDHVKISKQ-----WRLSPPTTTELCEAGVTIKVAKRKNNLCFMNISFK 379

Query: 298 NGVLKIPCLEIDCTFETVIRNVIAFDQYPAGNKNAYAIHYVLFLDDLINTEQDAHLLANV 357
           NGVL+IP + I+ TFE +IRNV+AF+ +PAGN+  YAI YV FLDDLI+TE+D  LL   
Sbjct: 380 NGVLEIPPIVIEGTFEVLIRNVLAFEIFPAGNQKKYAIQYVTFLDDLISTEKDLCLLVKA 439

Query: 358 GVIINILGGSDKDITEMFNNLSKYVTFPVCSHFDDISKALRKHCNKRWNKAIASLKHNYF 417
           GVIIN  GGSDK+++E+FN+L+K VT P+ S+FDD SKALR HC+  WNKA ASLKH+YF
Sbjct: 440 GVIINDTGGSDKEVSELFNSLTKLVTTPLPSYFDDTSKALRVHCDGSWNKAKASLKHSYF 499

Query: 418 NTPWAIISFLAATILIFLTILQTIFSAISTFPN 439
           NTPWAIISF AAT LI LTILQTIFSAIS FPN
Sbjct: 500 NTPWAIISFFAATFLIILTILQTIFSAISAFPN 523

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Y3720_ARATH5.8e-3425.64UPF0481 protein At3g47200 OS=Arabidopsis thaliana GN=At3g47200 PE=2 SV=1[more]
Y3264_ARATH2.9e-1726.70Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana GN=At3g02645 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0LPJ5_CUCSA1.9e-15375.95Uncharacterized protein OS=Cucumis sativus GN=Csa_2G380630 PE=4 SV=1[more]
A0A0A0LQ57_CUCSA5.3e-13556.81Uncharacterized protein OS=Cucumis sativus GN=Csa_2G380610 PE=4 SV=1[more]
A0A0A0LSF4_CUCSA2.3e-9846.48Uncharacterized protein OS=Cucumis sativus GN=Csa_2G380060 PE=4 SV=1[more]
A0A0A0LLX1_CUCSA1.2e-9444.72Uncharacterized protein OS=Cucumis sativus GN=Csa_2G380590 PE=4 SV=1[more]
A0A0A0LM96_CUCSA1.3e-9345.53Uncharacterized protein OS=Cucumis sativus GN=Csa_2G380080 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G31980.11.0e-4930.39 unknown protein[more]
AT3G50170.11.1e-4630.59 Plant protein of unknown function (DUF247)[more]
AT3G50150.11.3e-4430.42 Plant protein of unknown function (DUF247)[more]
AT3G50130.11.7e-4431.06 Plant protein of unknown function (DUF247)[more]
AT3G50120.11.1e-4329.10 Plant protein of unknown function (DUF247)[more]
Match NameE-valueIdentityDescription
gi|659088828|ref|XP_008445182.1|9.8e-16762.77PREDICTED: UPF0481 protein At3g47200-like [Cucumis melo][more]
gi|778674385|ref|XP_011650198.1|2.5e-16276.72PREDICTED: UPF0481 protein At3g47200-like [Cucumis sativus][more]
gi|700207815|gb|KGN62934.1|2.8e-15375.95hypothetical protein Csa_2G380630 [Cucumis sativus][more]
gi|700207813|gb|KGN62932.1|7.6e-13556.81hypothetical protein Csa_2G380610 [Cucumis sativus][more]
gi|778674382|ref|XP_004138863.2|3.9e-11558.02PREDICTED: UPF0481 protein At3g47200, partial [Cucumis sativus][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR004158DUF247_pln
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsa.162320Cucsa.162320gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsa.162320.1Cucsa.162320.1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.162320.1.CDS.4Cucsa.162320.1.CDS.4CDS
Cucsa.162320.1.CDS.3Cucsa.162320.1.CDS.3CDS
Cucsa.162320.1.CDS.2Cucsa.162320.1.CDS.2CDS
Cucsa.162320.1.CDS.1Cucsa.162320.1.CDS.1CDS


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004158Protein of unknown function DUF247, plantPFAMPF03140DUF247coord: 26..423
score: 1.2
NoneNo IPR availablePANTHERPTHR31549FAMILY NOT NAMEDcoord: 244..434
score: 1.0E-106coord: 27..228
score: 1.0E