Cucsa.159830.4 (mRNA) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.159830.4
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	tRNA (Adenine-N(1)-)-methyltransferase non-catalytic subunit trm6
Location	scaffold01144 : 202949 .. 208095 (-)
Sequence length	1781

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTCCAGGATTCAAAGGCTTGAACACGTCCGACTGAAGTTCGACTTTTCGATTCGGTCGAGCCCACTCACAGCACCCAGCGGTTAAAGCCCCCTCGTTCCCAGGTGTGCATTTCCTTGTCCCTGCTTCAACCGTTACTCTCTCTCTCCTTAAACCCTAAAACTCACTGAAAACCCATAGCAATTTCCCACTCTTTTGATCCTCTATACTTTTATGCTTTCTTAACTTATTCATCTTCTTAGTGTAGTTTCAAGATGTCCTTGACTATTCTTCAATCTGAATCGCGTCGAAACCCTAGATTGACATGGGAAGGTTGCAGTGTTTTGCTTGACGTCAATGATGGTGACCGCTTGGTTTTTGCTCGACTTTCTCCCGCCTCGTATGTCTCAAGTTTGAGTGTTGATTTCTGGTTGTCAATGCTGAGTAACTGTGATGCATAATAATGAAAATTACTTTGATTTGCAGTACGCTGAAAATTGGGAACAAAAGTTTCTCTCTTAAGCCTTTAATTGGTTGCCCATTCGGTTCCTTGTTTCAAATTGAAAATGGAGGGAATGGTCCAGAGCTGGCTCGCGTTATGCCTTCCAGAGAAGAAGAAGGTTTTGATTCATTACTGTGGATTTTGTTAATGAATTGGTTTCATCTTTGCGGAAAAAACGTTTAACTGAAGCGATGTGCTATTTGGGTATATTTTGTTTTTCAAGGCATTAGCTGTCAGGAAACGGAGGAGATGAAGGAAGAGCAGGTGACAAAAGATGAGCTGAGAGATAACAGAGCGATTGTAGACAATAATAAGGCACAAAGTCTCTCTAGTGAAGATATTGATGCAATGCGAAGGTAAGATATTGCTTTGCTTTGCTACTGGTTAAGTCGCATAATCTGTTTTATCCTGTCCCTCATATTCCCTTGAGAGGCACCCTTGGCTTTACCCTCTTAAAATGTTGATCCAAACACATTTTTATCACTGTGGATGAAGTCCAAAAGTCTTGAATATGACGAAATGCACTGGCAATTTCGGTCCTATTCCTATGCTGGGTATATGGGCATCATTATTATTTGAATTGGTTGGTTTCATAAAAAGAGCGACCAAACTTTGCAGCCCAATGAACCTGCTTGAACCCTGCATTTGTCACAGTGATTCTAGCTCTGTGGTATCTCAGTTGCAGGAAGCCAACCATGTGCGACCACTGTACTAGGATTTCAGTGGCACGGGGTCTGTTCCGCATATTCTCCTCATTGTTTTGAAATTGATGGGAATTGATCTTCTTTGATTTATTTTACAGAACTTTCCGTTGTCTTTTTCTTAAATATGTTATTATCCATGATGGATGCTGTCTCTTTATATGTAGTCAGGGTGCAACTGGTGATGAAATTGTGGAAGCTCTCATTGCCAACAGTGCAACATTTGATAAGAAAACAGTTTTCTCTCAGGTGATCAGAATGTGCATACCTGAGTAGCTTCATCTTTGTATGTAGCATTTTCTAAAGTTGTCCAAATTTTACATGCAGGAAAAATATAGGCTCAAGAAACAAAAGAAGTATGCTCCTAGGGTACTTTTGAGAAGACCTTTTACTCGAAGGTATACATAATAATAGTAATCCACTTAAAAAGTAAACGCACGGTAGATCACAGTCACTTTTTGTAGCATCACTTGAATCACTTGAATTCGTACATTAACAATGCTGATATTTCCCCCCGTCTTACAGTATATGTGAGGCATACTTCAAGAAATATCCAGCTAGAATTGGGTAATTTATTTGTCCACTTTTTCACTACATTTTTTCACTTTCAGTTTTAATTTTTTCCACATTTTAATTTTATGTTTGTGCGCTTGCATAATAATGATCTGCTTGTTTTCTATTTCCTTGCTTTTGATTCAGATTCTTGCGTTTGGATGCTTTATCTCTTCTTCTTTCATTTGGCAATGTGACTGCAAACTCTGATGTCCTTGTGGTTGATATGGTTGGTGGTCTTCTCACTGGAGCAGTGGCTGAGCGCCTAGGAGGTATTTCTCTATTATTCTTGGCATTTTAAAAAAAATATTCGAAGTGGGTTCATTTTTTTCAAAAAGTGGCTGCAAATCCTCTATGAATGTCAGTCATGGTACAAATCTAGGTTTATATGTGTGTAATGAAAAAACCTTGGGTATAAACCAACTACCAAGTGGGCTTAAAATCACTTGTCTCAAGCAAAGTTAGGTCTAATGGTTCAGCTAATCAGTCATGTTTATAGTTTCTTTGCTGTTAAGTGTATTTTCTAGTCACAAATAGTGTAGGAAACACTGTCGGTTGTCCATCCAATACGCTGGTTTTGAGGTAAGGCTTACAATATTTGGAATTCCTTCAAGGTCGCAAGTGTGTTATACCTTGTTGAAAACTTAACAGCTTAAAAACCATGTTTTATGGCGTTTGGGGTGAATGAAGTCTCTTTCACCATTAGTTTGAGTTGTCCATAGGCATAAATCAAATAAACAGGGGAAATCTATTGTTCGGTCTCAAGTTGCCATATGATAATTACAATGTTGTGTGCTTCTCTATATTATATAATGCTCAATTGTGTTTTTTTACAGGCAAAGGCTCTGTTTGCAATACATATGTTGGAAATACTCCAAACCCCATGGATATAGTAAGAATATTCAACTTTGATGATGAAATTTGCTCGAGGTTTGCTGCTTTAGTCCTCTTTCTTACATCTTCGTTACATTGCATATTTTATAAAATGTACTTTCTATTTTCTATTTTCTACACCATTCAATCACTCTTTGTTCACCAGGATAATGCGTTCATCTCTTGCCGACCTCACCTCATCCCAAATTGAACCTACCAAGCCTAATGGTGAAAACGAAGACAGTGTAAGTAGAGTTTTTCTTTTCTTAATGTTTTCTTAAGAGGAATTTGGAAGGAGCTGACTTCTGGAACGCTGAAGAGGGCTCTTGACTGAGTGCTTTTCAAAATTTGAAGTATTTGATACTGCAATCACAGCTACGGGACACAACGAATCACTTTCTTTATTGGGATACATCTTTTTATATTTAAAACATAAGTTTCTATCTATATTTATTTATTCAAGTCACCAAAACAAAGAATCTGTGCTTACTTAATTTAGTCCTTCCAAAAGTACTAGTTTAAATTTGTTACTCTTAGAATTCATTTTAAACTGTTTTGGTTGGATGATACCTTCTTTTTTTGTTCTTTATTATTTCTTTTTTTATAAAGATTAATGTACAGGAGCAGGCATCTCAACCTGTTAGTATGGACGAAAGCCCTCTACCATCTACAGACCAGTCCTCAGATCTCAACTCAGAGTCTATAGTTTCTCCATTGACAAAAGTATTTAAATGTGCAAAGGCTGGAGATAAGGCACCGCCAGAAGTTATTAATTTATGGAAGGAAAACGGTTTTACTAGGTGAGTAATGAGTATTGGTTAGTGGTAAAATTTACTTCACTATCAATAGTAATTCACTAAATTGTTTTTTATTAATTGCAGCCTAATAATTGCTGCACCACAGTCGGATGCATGGAGTCTTGTTAAAGATCTGTTACCTCTTGTAGCTAACTCTGCTCCATTTGCTATTTATCACCAGTATGTTCAGGTTGGTCTTCAAAAGAAGAAAAGAAATATTGTAAAAAAATGTTCGTTAAATTTACCTTACCTTCTCAAGGAGGGCTCTAAAGGCTTGGTGTATCTGAACGTCATAATTTAATACCCTTATGTCAGGCATGTTTAGCAGTAATTCTAATAAGGTTAAACTCACATTTTTCATTTTAAAAATCAATTATGAAACTTGCTTTTAATGATTCAAAACCAATTTTGATGAAATGAAAATTGCATTTAAAAGGTATAAATTTAAATATTAAATTTATTTTGACTGATTAAAGTCTTGTTTAGCATGATTTTGAGCTTGATAAAAGTGATTTTAAGAATATATTAGAGTAAAGATAGTCTCTCTGAGAATTAGCAGGGGACAAACCATGAGCATGATGATAGTAGGAGTGGAGCTCAATCCCCTCGTTATAAAATAAAGATATCAAGATATCGTTATTATTATTACCAAATGGAGATCCAAATAGAAATGAGATGTCATGTTTTGGGGGAGTGATTTTATATCCTTGTTTCCTTTTCAATAGGGGGAGTGATTTTATATCAATGTTATATATGTGTGTATCAGTTTGATTAAGTTACTTTTTTGTTGAATGCAGCCGCTTGCAACTTGCATGCATAAACTACAGCTTGAGAAGATGGCAATCAGCTTGCAAATTTCAGAACCTTGGCTTCGTGAATATCAGGTTTGATGGCTTTACATTTCCCCATACTTCCCTTTTAACAAAGTTTTGGAGAGAGGATTGAAGAACAATAGTCGTCCGCATTTAAACGTGGGATTTATACGTTCAAGATATTTACAAAGTCTTGACTTGCATGAAAATTACATAGGTCTGATTGCCCAATGAAAAGAAAATGTCTTGACTTGCATGGTTTGACTAACTGGTGGTAGTTGGTGAAAGTGTGGAAAGGTAAAAAGGGTTTGGGAATTGTTTCAAAAATCAAATTCTATGTGGGATCCATTTTGGATTTTAGAATCCTATGAAGTCTTTAAACAACCCTAAGTTATAGGATCGAGGTGACATGAATGAAAAATGAAAAATTAAGGTTCCGTTGGGTAACTAATTAAGCTTATTTATTTCCTCTAAGTTCCTATAAAATAGATGTGACAATTTTATTAACAGAACTTATGCTAAAGTTTATTTTATATGAAGGTTGAAGGTACAATATCTTTCATTTCTAATATCGTTGAATTGACCAATATTTGGCGCAATAATGCATGCAGGTTCTTCCTTCGAGAACTCATCCCTTTATGCAGATGAATGCATTTGGTGGATACATTCTTAGTGGGACTAAGATATGCGACCGTCAATAACAATTTCAATGTGTCGCCATTTTAGTGCAACTTTAAAATGCAGTGAAGGTGAAAACCAAATTTTGCCATGGGCTTGTATTTCAGCTTCTCTTAATTAGTTTTGTATCTTTCCATTTTTATTTAATCACTAGGATTACACAGCTCAGTAAACATCCTTTTTCTTTTAAATTATTTCACATAAAACCCATGTATCAATTTTATTATTATGATTATGAAAGGGGGATCGAGGCAC

mRNA sequence

GTCCAGGATTCAAAGGCTTGAACACGTCCGACTGAAGTTCGACTTTTCGATTCGGTCGAGCCCACTCACAGCACCCAGCGGTTAAAGCCCCCTCGTTCCCAGTTTCAAGATGTCCTTGACTATTCTTCAATCTGAATCGCGTCGAAACCCTAGATTGACATGGGAAGGTTGCAGTGTTTTGCTTGACGTCAATGATGGTGACCGCTTGGTTTTTGCTCGACTTTCTCCCGCCTCTACGCTGAAAATTGGGAACAAAAGTTTCTCTCTTAAGCCTTTAATTGGTTGCCCATTCGGTTCCTTGTTTCAAATTGAAAATGGAGGGAATGGTCCAGAGCTGGCTCGCGTTATGCCTTCCAGAGAAGAAGAAGGCATTAGCTGTCAGGAAACGGAGGAGATGAAGGAAGAGCAGGTGACAAAAGATGAGCTGAGAGATAACAGAGCGATTGTAGACAATAATAAGGCACAAAGTCTCTCTAGTGAAGATATTGATGCAATGCGAAGTCAGGGTGCAACTGGTGATGAAATTGTGGAAGCTCTCATTGCCAACAGTGCAACATTTGATAAGAAAACAGTTTTCTCTCAGGAAAAATATAGGCTCAAGAAACAAAAGAAGTATGCTCCTAGGGTACTTTTGAGAAGACCTTTTACTCGAAGTATATGTGAGGCATACTTCAAGAAATATCCAGCTAGAATTGGATTCTTGCGTTTGGATGCTTTATCTCTTCTTCTTTCATTTGGCAATGTGACTGCAAACTCTGATGTCCTTGTGGTTGATATGGTTGGTGGTCTTCTCACTGGAGCAGTGGCTGAGCGCCTAGGAGGCAAAGGCTCTGTTTGCAATACATATGTTGGAAATACTCCAAACCCCATGGATATAGTAAGAATATTCAACTTTGATGATGAAATTTGCTCGAGGATAATGCGTTCATCTCTTGCCGACCTCACCTCATCCCAAATTGAACCTACCAAGCCTAATGGTGAAAACGAAGACAGTATTAATGTACAGGAGCAGGCATCTCAACCTGTTAGTATGGACGAAAGCCCTCTACCATCTACAGACCAGTCCTCAGATCTCAACTCAGAGTCTATAGTTTCTCCATTGACAAAAGTATTTAAATGTGCAAAGGCTGGAGATAAGGCACCGCCAGAAGTTATTAATTTATGGAAGGAAAACGGTTTTACTAGGTGAGTAATGAGTATTGGTTAGTGGTAAAATTTACTTCACTATCAATAGTAATTCACTAAATTGTTTTTTATTAATTGCAGCCTAATAATTGCTGCACCACAGTCGGATGCATGGAGTCTTGTTAAAGATCTGTTACCTCTTGTAGCTAACTCTGCTCCATTTGCTATTTATCACCAGTATGTTCAGCCGCTTGCAACTTGCATGCATAAACTACAGCTTGAGAAGATGGCAATCAGCTTGCAAATTTCAGAACCTTGGCTTCGTGAATATCAGGTTCTTCCTTCGAGAACTCATCCCTTTATGCAGATGAATGCATTTGGTGGATACATTCTTAGTGGGACTAAGATATGCGACCGTCAATAACAATTTCAATGTGTCGCCATTTTAGTGCAACTTTAAAATGCAGTGAAGGTGAAAACCAAATTTTGCCATGGGCTTGTATTTCAGCTTCTCTTAATTAGTTTTGTATCTTTCCATTTTTATTTAATCACTAGGATTACACAGCTCAGTAAACATCCTTTTTCTTTTAAATTATTTCACATAAAACCCATGTATCAATTTTATTATTATGATTATGAAAGGGGGATCGAGGCAC

Coding sequence (CDS)

ATGTCCTTGACTATTCTTCAATCTGAATCGCGTCGAAACCCTAGATTGACATGGGAAGGTTGCAGTGTTTTGCTTGACGTCAATGATGGTGACCGCTTGGTTTTTGCTCGACTTTCTCCCGCCTCTACGCTGAAAATTGGGAACAAAAGTTTCTCTCTTAAGCCTTTAATTGGTTGCCCATTCGGTTCCTTGTTTCAAATTGAAAATGGAGGGAATGGTCCAGAGCTGGCTCGCGTTATGCCTTCCAGAGAAGAAGAAGGCATTAGCTGTCAGGAAACGGAGGAGATGAAGGAAGAGCAGGTGACAAAAGATGAGCTGAGAGATAACAGAGCGATTGTAGACAATAATAAGGCACAAAGTCTCTCTAGTGAAGATATTGATGCAATGCGAAGTCAGGGTGCAACTGGTGATGAAATTGTGGAAGCTCTCATTGCCAACAGTGCAACATTTGATAAGAAAACAGTTTTCTCTCAGGAAAAATATAGGCTCAAGAAACAAAAGAAGTATGCTCCTAGGGTACTTTTGAGAAGACCTTTTACTCGAAGTATATGTGAGGCATACTTCAAGAAATATCCAGCTAGAATTGGATTCTTGCGTTTGGATGCTTTATCTCTTCTTCTTTCATTTGGCAATGTGACTGCAAACTCTGATGTCCTTGTGGTTGATATGGTTGGTGGTCTTCTCACTGGAGCAGTGGCTGAGCGCCTAGGAGGCAAAGGCTCTGTTTGCAATACATATGTTGGAAATACTCCAAACCCCATGGATATAGTAAGAATATTCAACTTTGATGATGAAATTTGCTCGAGGATAATGCGTTCATCTCTTGCCGACCTCACCTCATCCCAAATTGAACCTACCAAGCCTAATGGTGAAAACGAAGACAGTATTAATGTACAGGAGCAGGCATCTCAACCTGTTAGTATGGACGAAAGCCCTCTACCATCTACAGACCAGTCCTCAGATCTCAACTCAGAGTCTATAGTTTCTCCATTGACAAAAGTATTTAAATGTGCAAAGGCTGGAGATAAGGCACCGCCAGAAGTTATTAATTTATGGAAGGAAAACGGTTTTACTAGGTGA

Protein sequence

MSLTILQSESRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCPFGSLFQIENGGNGPELARVMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQSLSSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFTRSICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKGSVCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENEDSINVQEQASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTR*

Homology

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BLAST of Cucsa.159830.4 vs. Swiss-Prot
Match: TRM6_DICDI (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 OS=Dictyostelium discoideum GN=trmt6 PE=3 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 3.4e-40
Identity = 108/328 (32.93%), Postives = 175/328 (53.35%), Query Frame = 1


Query: 19  EGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCPFGSLFQIENGGNGPELAR 78
           EG  V+LD+N+G++    ++   S +KIG K   +  +IG  + S FQ+ N  N   L R
Sbjct: 37  EGDHVILDINNGEKFSVIKVKLGSKVKIGKKQILINSIIGESYYSSFQVSNEKN--TLER 96

Query: 79  VMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQSLSSEDIDAMRSQGATGDE 138
           +     ++ ++     E+ +         DNR +  NN AQ L+ EDI+ M+ +G   + 
Sbjct: 97  ITQKEIDDRLN--NLVELNQNDA------DNRNLDQNNTAQKLTQEDINEMKQKGTDSNT 156

Query: 139 IVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFTRSICEAYFKKYPARIGFL 198
           I++ ++ NS +F  KT FSQ KY  KK KKY+  V + +P  +S+ EAY+KK   +I  L
Sbjct: 157 IIKTIVENSESFKTKTSFSQIKYLKKKIKKYSTIVKIIKPTLKSLTEAYYKKDSRKICGL 216

Query: 199 RLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKGSVCNTYVGNTPNPMDIVR 258
           R D+   LL+ GN+ ANS VLVV+   GL+TG++AER+ G+G++ + Y+G  P+ + IV 
Sbjct: 217 RFDSFGQLLTLGNIRANSQVLVVETCMGLVTGSIAERMNGQGTILSAYIGKGPS-LSIVN 276

Query: 259 IFNFDDEICSRIMRSSLADLTS--------SQIEPTKPNG-------ENEDSINVQEQAS 318
            F F+ ++ + I   +L ++TS        S++ PT  +G       E E      E   
Sbjct: 277 NFGFNTDVLNTIYPFNL-NITSVLNKGEDISKLPPTASSGIYDKQVKEKEKEKEKDENVK 336

Query: 319 QPVSMDESPLPSTDQSSDLNSESIVSPL 332
                 E      +Q +D ++E+IV  L
Sbjct: 337 DEKESGEEAKTIINQRNDTSNENIVKLL 352

[Close]

BLAST of Cucsa.159830.4 vs. Swiss-Prot
Match: TRM6_BOVIN (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Bos taurus GN=TRMT6 PE=2 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 4.7e-34
Identity = 96/324 (29.63%), Postives = 160/324 (49.38%), Query Frame = 1


Query: 27  VNDGDRLVFARLSPASTLKIGNKS--------FSLKPLIGCPFGSLFQIENGGNGPELAR 86
           + DGD +V  R      +++  +         F L  +IG  +G+ F++ NGG+      
Sbjct: 20  IRDGDFVVLKREDVFKAVQVQRRKKVTFEKQWFYLDNIIGHSYGTTFEVTNGGS------ 79

Query: 87  VMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQSLSSEDIDAMRSQGATGDE 146
           + P +++E    + T E KE         DNR I+D+ K+Q L+ +DI A++ +G  G+E
Sbjct: 80  LQPKKKKE----EPTSETKEAGT------DNRNIIDDGKSQKLTQDDIKALKDKGIKGEE 139

Query: 147 IVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFTRSICEAYFKKYPARIGFL 206
           IV+ LI NS TF  KT F+Q+KY  KK+KKY   + + +P TR +   Y+ + P +I  +
Sbjct: 140 IVQQLIENSTTFRDKTEFAQDKYIKKKKKKYEAMITVVKPSTRILSVMYYAREPGKINHM 199

Query: 207 RLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKGSVCNTYVGNTP------- 266
           R D L+ +L+ GN+ A + ++V++   GL+ GA+ ER+GG GS+   Y G  P       
Sbjct: 200 RYDTLAQMLTLGNIRAGNKMIVMETCAGLVLGAMMERMGGFGSIIQLYPGGGPVRAATAC 259

Query: 267 ----------------NPMDIVRIFNFDDEICSRIMR--SSLADLTSSQIEPTKPNGENE 318
                           N +D +    F  E+ S   +  +S+ +   +  E      ENE
Sbjct: 260 FGFPKSFLSGLYEFPLNKVDSLLNGTFSAEMLSSEPKDIASVEESNGTLEEKQTSEQENE 319

[Close]

BLAST of Cucsa.159830.4 vs. Swiss-Prot
Match: TRM6_MOUSE (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Mus musculus GN=Trmt6 PE=1 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 1.2e-32
Identity = 99/317 (31.23%), Postives = 159/317 (50.16%), Query Frame = 1


Query: 27  VNDGDRLVFARLSPASTLKIGNKS--------FSLKPLIGCPFGSLFQIENGGNGPELAR 86
           ++DGD +V  R      +++  +         F L   IG  +GS F + +GG       
Sbjct: 21  IHDGDFVVLKREDVFKAVQVQRRKKVTFEKQWFYLDNAIGHSYGSAFDVSSGG------- 80

Query: 87  VMPSREEEGISCQETEEMKEE-QVTKDELRDNRAIVDNNKAQSLSSEDIDAMRSQGATGD 146
                     S Q  ++++E    TK+   DNR IVD+ K+Q L+ +DI A++ +G  G+
Sbjct: 81  ----------SLQLRKKLEEPASETKEAGTDNRNIVDDGKSQKLTQDDIKALKDKGIKGE 140

Query: 147 EIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFTRSICEAYFKKYPARIGF 206
           EIV+ LI NS TF  KT F+Q+KY  KK+KKY   V + +P TR +   Y+ + P +I  
Sbjct: 141 EIVQQLIENSTTFRDKTEFAQDKYIKKKKKKYEAIVTILKPSTRILSIMYYAREPGKINH 200

Query: 207 LRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKGSVCNTYVGNTPNPMDIV 266
           +R D L+ +L+ GN+ A + ++V++   GL+ GA+ ER+GG GS+   Y G+ P      
Sbjct: 201 MRYDTLAQMLTLGNIRAGNKMIVMETCSGLVLGAMMERMGGFGSIIQLYPGDGP-VRAAT 260

Query: 267 RIFNFDDEICSRIMRSSL------------ADLTSSQIEPTKP----NGENEDSINVQEQ 319
             F F     S +    L            A++ SS+ + + P    NGE E+   + EQ
Sbjct: 261 ACFGFPKSFLSGLYEFPLNKVNSLLNGTFSAEMLSSEPKDSTPVEESNGELEEK-EIAEQ 318

[Close]

BLAST of Cucsa.159830.4 vs. Swiss-Prot
Match: TRM6_HUMAN (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Homo sapiens GN=TRMT6 PE=1 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 4.4e-32
Identity = 80/233 (34.33%), Postives = 130/233 (55.79%), Query Frame = 1


Query: 27  VNDGDRLVFARLSPASTLKIGNKS--------FSLKPLIGCPFGSLFQIENGGNGPELAR 86
           + DGD +V  R      +++  +         F L  +IG  +G+ F++ +GG+      
Sbjct: 20  IRDGDFVVLKREDVFKAVQVQRRKKVTFEKQWFYLDNVIGHSYGTAFEVTSGGS------ 79

Query: 87  VMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQSLSSEDIDAMRSQGATGDE 146
           + P ++ E    + T E KE         DNR IVD+ K+Q L+ +DI A++ +G  G+E
Sbjct: 80  LQPKKKRE----EPTAETKEAGT------DNRNIVDDGKSQKLTQDDIKALKDKGIKGEE 139

Query: 147 IVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFTRSICEAYFKKYPARIGFL 206
           IV+ LI NS TF  KT F+Q+KY  KK+KKY   + + +P TR +   Y+ + P +I  +
Sbjct: 140 IVQQLIENSTTFRDKTEFAQDKYIKKKKKKYEAIITVVKPSTRILSIMYYAREPGKINHM 199

Query: 207 RLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKGSVCNTYVGNTP 252
           R D L+ +L+ GN+ A + ++V++   GL+ GA+ ER+GG GS+   Y G  P
Sbjct: 200 RYDTLAQMLTLGNIRAGNKMIVMETCAGLVLGAMMERMGGFGSIIQLYPGGGP 236

[Close]

BLAST of Cucsa.159830.4 vs. Swiss-Prot
Match: TRM6_DEBHA (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) GN=TRM6 PE=3 SV=2)

HSP 1 Score: 117.5 bits (293), Expect = 3.1e-25
Identity = 79/280 (28.21%), Postives = 142/280 (50.71%), Query Frame = 1


Query: 2   SLTILQSESRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGN-KSFSLKPLIGCP 61
           S TI+        RL  EG  ++             L P   + +G   +F++  ++G P
Sbjct: 26  STTIISPNQHALIRLPSEGLKII------------DLKPGGIISLGKFGTFAVDGILGYP 85

Query: 62  FGSLFQIENGGNGPELARVMPSREEEGISCQETEEMKEEQVTK--DELRDNRAIVDN--N 121
           FG  F+I        +  +    +E  +   E EE+ ++ +TK      DN   + N  +
Sbjct: 86  FGQTFEILEELKVKPIKSMTMQADETSVD-NENEELTKDDLTKMLSNSSDNNQNIINIGS 145

Query: 122 KAQSLSSEDIDAMRSQGATGD---EIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRV 181
           K Q L+SED+D ++  GAT D    I+E +IA    FDKKT+FSQ+KY  +KQ+K+  R 
Sbjct: 146 KIQKLTSEDVDKLKESGATSDIGQRIIEQMIAGHEGFDKKTLFSQQKYLKRKQQKFLRRF 205

Query: 182 LLRRPFTRSICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVA 241
            +    +  + + Y +K   R+  +  + L LLL++ NV      L++D  GG++  A+ 
Sbjct: 206 TVEYLGSSQLLQYYIEKDTQRVLDMSEETLGLLLNYANVRPGGKYLLIDETGGIILYAMM 265

Query: 242 ERLGGKGSVCNTYVGNTPNPMDIVRIFNFDDEICSRIMRS 274
           ER+ G+G++ + +    PN +  +R  ++ +E+ +R+++S
Sbjct: 266 ERMNGQGTIVSAHDNEHPNHI-ALRYSDYPEEMQNRMVKS 291

[Close]

BLAST of Cucsa.159830.4 vs. TrEMBL
Match: A0A0A0LRN2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G367740 PE=4 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 1.3e-200
Identity = 358/358 (100.00%), Postives = 358/358 (100.00%), Query Frame = 1


Query: 1   MSLTILQSESRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP 60
           MSLTILQSESRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP
Sbjct: 1   MSLTILQSESRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP 60

Query: 61  FGSLFQIENGGNGPELARVMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQS 120
           FGSLFQIENGGNGPELARVMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQS
Sbjct: 61  FGSLFQIENGGNGPELARVMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQS 120

Query: 121 LSSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFT 180
           LSSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFT
Sbjct: 121 LSSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFT 180

Query: 181 RSICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKG 240
           RSICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKG
Sbjct: 181 RSICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKG 240

Query: 241 SVCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENEDSINVQE 300
           SVCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENEDSINVQE
Sbjct: 241 SVCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENEDSINVQE 300

Query: 301 QASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFT 359
           QASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFT
Sbjct: 301 QASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFT 358

[Close]

BLAST of Cucsa.159830.4 vs. TrEMBL
Match: M5XBI1_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005656mg PE=4 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 3.1e-125
Identity = 241/362 (66.57%), Postives = 278/362 (76.80%), Query Frame = 1


Query: 1   MSLTILQSESRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP 60
           MS    QS+   +PR TWEGCSVLLD+NDGDRLVFARLS  +TLKIGNK+ SL+PL+GCP
Sbjct: 1   MSENKAQSDLFESPRSTWEGCSVLLDINDGDRLVFARLSSGATLKIGNKNCSLQPLLGCP 60

Query: 61  FGSLFQIENGGNGPELARVMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQS 120
           FGS F++ENG  GP L+R  PS E   +        K +  + DELRDNRA+VDNNKAQS
Sbjct: 61  FGSFFRVENGTQGPYLSRYTPSTEGNNVL------EKGDGQSIDELRDNRALVDNNKAQS 120

Query: 121 LSSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFT 180
           L+ EDI+ MR  GATGDEIVEALIANSATF+KKTVFSQEKYRLKKQKKYAP VLLRRPFT
Sbjct: 121 LTGEDINEMRRLGATGDEIVEALIANSATFEKKTVFSQEKYRLKKQKKYAPIVLLRRPFT 180

Query: 181 RSICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKG 240
           RSICEAY KKYP RIGFLR+D LSLLLS  NV+ANSDVLVVDMVGGLLTG+VAERLGG G
Sbjct: 181 RSICEAYLKKYPTRIGFLRMDTLSLLLSMANVSANSDVLVVDMVGGLLTGSVAERLGGTG 240

Query: 241 SVCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENEDSINVQE 300
            VCNTY+G +P PMDI RIFNF DEIC RI+RS L DL S Q    KP  ++ DS N++ 
Sbjct: 241 YVCNTYIGGSPYPMDIARIFNFGDEICKRIVRSPLTDLFSVQNGLQKPVSQDSDSCNMES 300

Query: 301 QA----SQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENG 359
           Q+    S PVSM+E  L S +  SDL  ES  SP+ K+ K AK G+KAP E+I+ WKE+G
Sbjct: 301 QSNDRISSPVSMEEVSLASENVISDLIPESTSSPVIKMSKPAKLGEKAPQEIISSWKEHG 356

[Close]

BLAST of Cucsa.159830.4 vs. TrEMBL
Match: A0A067KYJ4_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_08225 PE=4 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 6.9e-125
Identity = 227/358 (63.41%), Postives = 285/358 (79.61%), Query Frame = 1


Query: 1   MSLTILQSESRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP 60
           MS   L+S+S +NPR+TWEGCSVLLD+NDGDRLVF+RL+P S LKIGNK+ SL+PLIGCP
Sbjct: 1   MSQNNLESDSLQNPRVTWEGCSVLLDINDGDRLVFSRLTPGSNLKIGNKNLSLRPLIGCP 60

Query: 61  FGSLFQIENGGNGPELARVMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQS 120
           FGSLFQIENG  GP L+R +PS++          E K +    DE RDNRA++DNNKAQS
Sbjct: 61  FGSLFQIENGKEGPCLSRFIPSKQGNNA------EEKSDCEMIDESRDNRALIDNNKAQS 120

Query: 121 LSSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFT 180
           L+ +DIDAMR QGA G+EI++ALIANSATF+KKT+FSQEKYRLKKQKKYAPRVLLRRPFT
Sbjct: 121 LTGDDIDAMRRQGAKGEEIIDALIANSATFEKKTLFSQEKYRLKKQKKYAPRVLLRRPFT 180

Query: 181 RSICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKG 240
           RSICE YFKKYPARIG+LR+DALSLLLS  NVTANSD+LV+DMVGGLLTGAVAERLGG G
Sbjct: 181 RSICETYFKKYPARIGYLRIDALSLLLSMANVTANSDILVMDMVGGLLTGAVAERLGGTG 240

Query: 241 SVCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENEDSINVQE 300
            VC+TY+G+TP+ +DIVR+FNF+++IC RI+R+SL+DL  +Q E +  +  +ED+     
Sbjct: 241 YVCSTYLGDTPHSVDIVRMFNFNNDICKRIVRASLSDLCLAQNETSNQSCAHEDAPMESN 300

Query: 301 QASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFT 359
             +  +SM E  L S  +++D++ +   SP +K+ K  KAG++A  E + LWKENGF+
Sbjct: 301 GQTSSISMAEMSLSSEPEAADIDPDPTASPASKISKVHKAGERATDEALKLWKENGFS 352

[Close]

BLAST of Cucsa.159830.4 vs. TrEMBL
Match: A0A061FE57_THECC (Eukaryotic initiation factor 3 gamma subunit family protein OS=Theobroma cacao GN=TCM_046884 PE=4 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 9.1e-125
Identity = 238/356 (66.85%), Postives = 279/356 (78.37%), Query Frame = 1


Query: 7   QSESRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCPFGSLFQ 66
           QS+S +NPR+TWEGCSVLLD+NDGDRLVFARLS  STLK GNK+ SL+PLIGCPFGSLFQ
Sbjct: 7   QSDSIQNPRVTWEGCSVLLDINDGDRLVFARLSAGSTLKFGNKTCSLQPLIGCPFGSLFQ 66

Query: 67  IENGGNGPELARVMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQSLSSEDI 126
           +E+G   P L+R +PS EE     +   ++KEE       RDNRAI+DNN+AQ L+ EDI
Sbjct: 67  VESGKEKPYLSRYIPSTEEGNAQDEGGCQLKEES------RDNRAIIDNNQAQCLTGEDI 126

Query: 127 DAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFTRSICEA 186
           DAM+ QGATG+EIVEALIANSATF+KKT FSQEKYRLKKQKKYAP+VLLRRPF+RSICEA
Sbjct: 127 DAMKRQGATGNEIVEALIANSATFEKKTQFSQEKYRLKKQKKYAPKVLLRRPFSRSICEA 186

Query: 187 YFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKGSVCNTY 246
           YFKKYPARIGFLR+DALSLLLS  NVTANSDVLVVDMVGGLLTGAVAERLGG GS+CNTY
Sbjct: 187 YFKKYPARIGFLRVDALSLLLSMANVTANSDVLVVDMVGGLLTGAVAERLGGTGSLCNTY 246

Query: 247 VGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENEDSINVQ----EQA 306
            G TP PM+I+R+FNF+ EIC RI+R S  DL S+Q    +  G+ +D   V+    EQ 
Sbjct: 247 PGGTPYPMEIIRMFNFNSEICKRILRCSPKDLCSAQNGTDEQVGQQKDVSTVESQSNEQT 306

Query: 307 SQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFT 359
           S  VS +E  L S +  +DL  E+ +S ++K  K  KAGDKAP E I LWKENGF+
Sbjct: 307 SLSVSTEEVHLSSENNVADLVPENELSTVSKTCKAPKAGDKAPKEAIQLWKENGFS 356

[Close]

BLAST of Cucsa.159830.4 vs. TrEMBL
Match: A0A0D2UQE3_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_009G195100 PE=4 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 1.0e-123
Identity = 230/350 (65.71%), Postives = 279/350 (79.71%), Query Frame = 1


Query: 13  NPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCPFGSLFQIENGGN 72
           NPR+TWEGCSVLLD+NDGDRL+FARLS  STLKIGNK+FSL+PLIGCPFGSLFQ+E+G  
Sbjct: 49  NPRVTWEGCSVLLDINDGDRLLFARLSAGSTLKIGNKTFSLQPLIGCPFGSLFQVESGKE 108

Query: 73  GPELARVMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQSLSSEDIDAMRSQ 132
            P L+R +P  EE  +  +   +++EE       RDNRAI+DNN+AQSL+ +DIDAMR Q
Sbjct: 109 KPYLSRFIPPTEENNVQDEGGCQLQEES------RDNRAIIDNNQAQSLTGDDIDAMRRQ 168

Query: 133 GATGDEIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFTRSICEAYFKKYP 192
           GATG+EIVEALIANSATFDKKT FSQEKYRLKKQKKYAPRVLLRRPF+RSICEAYFKKYP
Sbjct: 169 GATGNEIVEALIANSATFDKKTQFSQEKYRLKKQKKYAPRVLLRRPFSRSICEAYFKKYP 228

Query: 193 ARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKGSVCNTYVGNTPN 252
           ARIGFLR+DALSLLLS  NVTANSDVLV+DMVGGL+TGAVAERLGG GSVCNTY+G TP 
Sbjct: 229 ARIGFLRVDALSLLLSMANVTANSDVLVLDMVGGLVTGAVAERLGGTGSVCNTYLGGTPY 288

Query: 253 PMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENEDSINVQEQASQPVSM---- 312
           PM+I+R+FNF++EIC R++R S+ DL + Q E ++   ++ED   ++ Q+ + +S+    
Sbjct: 289 PMEIIRMFNFNNEICKRVLRCSVNDLCAVQNETSEQVSQHEDVCTMESQSDEKISLSVST 348

Query: 313 DESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFT 359
           +E  L S +  SDL  E+ +S   K  K  KAG+KAP E I  WKENGF+
Sbjct: 349 EEVHLSSKNGVSDLVPENELSTTGKTCKTPKAGEKAPKEAIQSWKENGFS 392

[Close]

BLAST of Cucsa.159830.4 vs. TAIR10
Match: AT2G45730.1 (AT2G45730.1 eukaryotic initiation factor 3 gamma subunit family protein)

HSP 1 Score: 396.7 bits (1018), Expect = 1.5e-110
Identity = 205/350 (58.57%), Postives = 260/350 (74.29%), Query Frame = 1


Query: 13  NPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCPFGSLFQIENGGN 72
           NPR   EGCSVLLD+NDGDRLVFARLS  + LKIGNK++SLKPLIG PFGSLFQ+E G +
Sbjct: 20  NPRFACEGCSVLLDINDGDRLVFARLSSGAILKIGNKNYSLKPLIGAPFGSLFQVETGED 79

Query: 73  GPELARVMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQSLSSEDIDAMRSQ 132
           G  L+R++P ++E                  D+ RDNR IVDNN+AQ+L+ E+I+AMR +
Sbjct: 80  GSFLSRILPIKQESN-----------SNNVMDDSRDNREIVDNNEAQNLTGEEIEAMRRE 139

Query: 133 GATGDEIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFTRSICEAYFKKYP 192
           GA GDEI+EALIANS TFDKK   SQEKY+LKKQKKYAP++LLRRPF RSICEAYFKKYP
Sbjct: 140 GAKGDEIIEALIANSKTFDKKFQLSQEKYKLKKQKKYAPKLLLRRPFARSICEAYFKKYP 199

Query: 193 ARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKGSVCNTYVGNTPN 252
           ARIGFLR+DALSLLL+  NVTA SDVLVVDMVGGL+TGAVAERLGG G VCNTY G++P 
Sbjct: 200 ARIGFLRVDALSLLLTMANVTAYSDVLVVDMVGGLVTGAVAERLGGTGYVCNTYKGSSPY 259

Query: 253 PMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENED----SINVQEQASQPVSM 312
           P+++VR+FNF D++  RI++SS+ +L+S++   T+ N + E       N+ E A+  V +
Sbjct: 260 PVEMVRMFNFSDKVLKRIVQSSINELSSAKTASTEGNNQQEGVCKAENNINETATSDVMV 319

Query: 313 DESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFT 359
           +E  +     +++     IV P +K+ K AKAG KA  E I +W+ENGF+
Sbjct: 320 EEISV-----TTEAGVVDIVVPESKIIKAAKAGAKASKETIEMWQENGFS 353

[Close]

BLAST of Cucsa.159830.4 vs. NCBI nr
Match: gi|778671553|ref|XP_011649655.1| (PREDICTED: tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 isoform X1 [Cucumis sativus])

HSP 1 Score: 706.8 bits (1823), Expect = 1.9e-200
Identity = 358/358 (100.00%), Postives = 358/358 (100.00%), Query Frame = 1


Query: 1   MSLTILQSESRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP 60
           MSLTILQSESRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP
Sbjct: 1   MSLTILQSESRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP 60

Query: 61  FGSLFQIENGGNGPELARVMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQS 120
           FGSLFQIENGGNGPELARVMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQS
Sbjct: 61  FGSLFQIENGGNGPELARVMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQS 120

Query: 121 LSSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFT 180
           LSSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFT
Sbjct: 121 LSSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFT 180

Query: 181 RSICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKG 240
           RSICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKG
Sbjct: 181 RSICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKG 240

Query: 241 SVCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENEDSINVQE 300
           SVCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENEDSINVQE
Sbjct: 241 SVCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENEDSINVQE 300

Query: 301 QASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFT 359
           QASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFT
Sbjct: 301 QASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFT 358

[Close]

BLAST of Cucsa.159830.4 vs. NCBI nr
Match: gi|778671560|ref|XP_011649657.1| (PREDICTED: tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 isoform X2 [Cucumis sativus])

HSP 1 Score: 700.3 bits (1806), Expect = 1.8e-198
Identity = 357/358 (99.72%), Postives = 357/358 (99.72%), Query Frame = 1


Query: 1   MSLTILQSESRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP 60
           MSLTILQSESRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP
Sbjct: 1   MSLTILQSESRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP 60

Query: 61  FGSLFQIENGGNGPELARVMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQS 120
           FGSLFQIENGGNGPELARVMPSRE EGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQS
Sbjct: 61  FGSLFQIENGGNGPELARVMPSRE-EGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQS 120

Query: 121 LSSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFT 180
           LSSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFT
Sbjct: 121 LSSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFT 180

Query: 181 RSICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKG 240
           RSICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKG
Sbjct: 181 RSICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKG 240

Query: 241 SVCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENEDSINVQE 300
           SVCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENEDSINVQE
Sbjct: 241 SVCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENEDSINVQE 300

Query: 301 QASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFT 359
           QASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFT
Sbjct: 301 QASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFT 357

[Close]

BLAST of Cucsa.159830.4 vs. NCBI nr
Match: gi|449469893|ref|XP_004152653.1| (PREDICTED: tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 isoform X4 [Cucumis sativus])

HSP 1 Score: 693.7 bits (1789), Expect = 1.7e-196
Identity = 354/358 (98.88%), Postives = 354/358 (98.88%), Query Frame = 1


Query: 1   MSLTILQSESRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP 60
           MSLTILQSESRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP
Sbjct: 1   MSLTILQSESRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP 60

Query: 61  FGSLFQIENGGNGPELARVMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQS 120
           FGSLFQIENGGNGPELARVMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQS
Sbjct: 61  FGSLFQIENGGNGPELARVMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQS 120

Query: 121 LSSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFT 180
           LSSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFT
Sbjct: 121 LSSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFT 180

Query: 181 RSICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKG 240
           RSICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKG
Sbjct: 181 RSICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKG 240

Query: 241 SVCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENEDSINVQE 300
           SVCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENEDS    E
Sbjct: 241 SVCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENEDS----E 300

Query: 301 QASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFT 359
           QASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFT
Sbjct: 301 QASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFT 354

[Close]

BLAST of Cucsa.159830.4 vs. NCBI nr
Match: gi|659088089|ref|XP_008444796.1| (PREDICTED: tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 isoform X1 [Cucumis melo])

HSP 1 Score: 682.6 bits (1760), Expect = 3.8e-193
Identity = 346/357 (96.92%), Postives = 353/357 (98.88%), Query Frame = 1


Query: 2   SLTILQSESRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCPF 61
           S TILQS+SRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCPF
Sbjct: 3   STTILQSDSRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCPF 62

Query: 62  GSLFQIENGGNGPELARVMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQSL 121
           GSLFQIENG NGP+LARVMPSREE GI+CQETEEMKEEQVTKDELRDNRA+VDNNKAQSL
Sbjct: 63  GSLFQIENGENGPQLARVMPSREE-GINCQETEEMKEEQVTKDELRDNRALVDNNKAQSL 122

Query: 122 SSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFTR 181
           SSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRL+KQKKYAPRVLLRRPFTR
Sbjct: 123 SSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLRKQKKYAPRVLLRRPFTR 182

Query: 182 SICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKGS 241
           SICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKGS
Sbjct: 183 SICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKGS 242

Query: 242 VCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENEDSINVQEQ 301
           VCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGEN+ S+NVQEQ
Sbjct: 243 VCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENKVSVNVQEQ 302

Query: 302 ASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFT 359
           ASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFT
Sbjct: 303 ASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFT 358

[Close]

BLAST of Cucsa.159830.4 vs. NCBI nr
Match: gi|659088095|ref|XP_008444799.1| (PREDICTED: tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 isoform X3 [Cucumis melo])

HSP 1 Score: 671.0 bits (1730), Expect = 1.2e-189
Identity = 343/357 (96.08%), Postives = 349/357 (97.76%), Query Frame = 1


Query: 2   SLTILQSESRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCPF 61
           S TILQS+SRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCPF
Sbjct: 3   STTILQSDSRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCPF 62

Query: 62  GSLFQIENGGNGPELARVMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQSL 121
           GSLFQIENG NGP+LARVMPSREE GI+CQETEEMKEEQVTKDELRDNRA+VDNNKAQSL
Sbjct: 63  GSLFQIENGENGPQLARVMPSREE-GINCQETEEMKEEQVTKDELRDNRALVDNNKAQSL 122

Query: 122 SSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFTR 181
           SSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRL+KQKKYAPRVLLRRPFTR
Sbjct: 123 SSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLRKQKKYAPRVLLRRPFTR 182

Query: 182 SICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKGS 241
           SICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKGS
Sbjct: 183 SICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKGS 242

Query: 242 VCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENEDSINVQEQ 301
           VCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGEN+    V EQ
Sbjct: 243 VCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENK----VSEQ 302

Query: 302 ASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFT 359
           ASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFT
Sbjct: 303 ASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFT 354

The following BLAST results are available for this feature:

BLAST of Cucsa.159830.4 vs. Swiss-Prot


Match Name	E-value	Identity	Description
TRM6_DICDI	3.4e-40	32.93	tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 OS=Dictyost...	[more]
TRM6_BOVIN	4.7e-34	29.63	tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Bos taur...	[more]
TRM6_MOUSE	1.2e-32	31.23	tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Mus musc...	[more]
TRM6_HUMAN	4.4e-32	34.33	tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Homo sap...	[more]
TRM6_DEBHA	3.1e-25	28.21	tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Debaryom...	[more]

BLAST of Cucsa.159830.4 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LRN2_CUCSA	1.3e-200	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G367740 PE=4 SV=1	[more]
M5XBI1_PRUPE	3.1e-125	66.57	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005656mg PE=4 SV=1	[more]
A0A067KYJ4_JATCU	6.9e-125	63.41	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_08225 PE=4 SV=1	[more]
A0A061FE57_THECC	9.1e-125	66.85	Eukaryotic initiation factor 3 gamma subunit family protein OS=Theobroma cacao G...	[more]
A0A0D2UQE3_GOSRA	1.0e-123	65.71	Uncharacterized protein OS=Gossypium raimondii GN=B456_009G195100 PE=4 SV=1	[more]

BLAST of Cucsa.159830.4 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G45730.1	1.5e-110	58.57	eukaryotic initiation factor 3 gamma subunit family protein	[more]

BLAST of Cucsa.159830.4 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778671553\|ref\|XP_011649655.1\|	1.9e-200	100.00	PREDICTED: tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 ...	[more]
gi\|778671560\|ref\|XP_011649657.1\|	1.8e-198	99.72	PREDICTED: tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 ...	[more]
gi\|449469893\|ref\|XP_004152653.1\|	1.7e-196	98.88	PREDICTED: tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 ...	[more]
gi\|659088089\|ref\|XP_008444796.1\|	3.8e-193	96.92	PREDICTED: tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 ...	[more]
gi\|659088095\|ref\|XP_008444799.1\|	1.2e-189	96.08	PREDICTED: tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 ...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR017423	TRM6

Vocabulary: **Biological Process**
Term	Definition
GO:0030488	tRNA methylation

Vocabulary: **Cellular Component**
Term	Definition
GO:0031515	tRNA (m1A) methyltransferase complex

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030488	tRNA methylation
biological_process	GO:0044271	cellular nitrogen compound biosynthetic process
biological_process	GO:1901566	organonitrogen compound biosynthetic process
biological_process	GO:0006446	regulation of translational initiation
biological_process	GO:0044763	single-organism cellular process
cellular_component	GO:0031515	tRNA (m1A) methyltransferase complex
cellular_component	GO:0005840	ribosome
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0016740	transferase activity
molecular_function	GO:0003743	translation initiation factor activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsa.159830	Cucsa.159830	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsa.159830.4	Cucsa.159830.4-protein	polypeptide

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.159830.4.three_prime_UTR.3	Cucsa.159830.4.three_prime_UTR.3	three_prime_UTR
Cucsa.159830.4.three_prime_UTR.2	Cucsa.159830.4.three_prime_UTR.2	three_prime_UTR
Cucsa.159830.4.three_prime_UTR.1	Cucsa.159830.4.three_prime_UTR.1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.159830.4.CDS.10	Cucsa.159830.4.CDS.10	CDS
Cucsa.159830.4.CDS.9	Cucsa.159830.4.CDS.9	CDS
Cucsa.159830.4.CDS.8	Cucsa.159830.4.CDS.8	CDS
Cucsa.159830.4.CDS.7	Cucsa.159830.4.CDS.7	CDS
Cucsa.159830.4.CDS.6	Cucsa.159830.4.CDS.6	CDS
Cucsa.159830.4.CDS.5	Cucsa.159830.4.CDS.5	CDS
Cucsa.159830.4.CDS.4	Cucsa.159830.4.CDS.4	CDS
Cucsa.159830.4.CDS.3	Cucsa.159830.4.CDS.3	CDS
Cucsa.159830.4.CDS.2	Cucsa.159830.4.CDS.2	CDS
Cucsa.159830.4.CDS.1	Cucsa.159830.4.CDS.1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.159830.4.five_prime_UTR.2	Cucsa.159830.4.five_prime_UTR.2	five_prime_UTR
Cucsa.159830.4.five_prime_UTR.1	Cucsa.159830.4.five_prime_UTR.1	five_prime_UTR

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017423	tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6	PANTHER	PTHR12945	TRANSLATION INITIATION FACTOR EIF3-RELATED	coord: 1..357 score: 3.2E
IPR017423	tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6	PFAM	PF04189	Gcd10p	coord: 22..286 score: 1.1
None	No IPR available	PANTHER	PTHR12945:SF0	TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT TRM6	coord: 1..357 score: 3.2E