Cucsa.152620.1 (mRNA) Cucumber (Gy14) v1

BLAST of Cucsa.152620.1 vs. Swiss-Prot
Match: ASOL_BRANA (L-ascorbate oxidase homolog OS=Brassica napus GN=Bp10 PE=2 SV=1)

HSP 1 Score: 890.2 bits (2299), Expect = 1.1e-257
Identity = 408/535 (76.26%), Postives = 464/535 (86.73%), Query Frame = 1

Query: 2   TATVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNNLVINVFNNLD 61
           T  V  EDPYF   WNVTYGT SPLGVPQQ ILINGQFPGPNINSTSNNN++INVFNNLD
Sbjct: 18  TVVVHAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINSTSNNNVIINVFNNLD 77

Query: 62  EPFLLHWSGIQHRKNSWQDGLLGTNCPILPGTNYTYHFQVKDQIGSFFYYPSTAMHRAAG 121
           EPFLL W+GIQHRKN WQDG  GT CPI+PGTNYTYHFQ KDQIGS+FYYP+T MHRAAG
Sbjct: 78  EPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIGSYFYYPTTGMHRAAG 137

Query: 122 GFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRSIARPDGVLINGK 181
           G+GGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHT LK+ LD GR+I RPDG++INGK
Sbjct: 138 GYGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDGGRTIGRPDGIVINGK 197

Query: 182 SAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMKLVEMEGSHTVQNDYESL 241
           S KGDG+D PLFT+KPGKTY+ R+CNVG+K+S+NFR Q H MKLVEMEGSH +QNDY+SL
Sbjct: 198 SGKGDGSDAPLFTLKPGKTYRVRICNVGVKTSINFRIQNHKMKLVEMEGSHVLQNDYDSL 257

Query: 242 DVHVGQCFSVLVTANQEPKDYYMVASTRFIKNVLVGKGIVRYIDGKGPASPEIPEAPMGW 301
           DVHVGQCF  +VTANQEPKDYYMVAS+RF+K V+   G++RY  GKGPAS ++P  P+GW
Sbjct: 258 DVHVGQCFGTIVTANQEPKDYYMVASSRFLKTVITTTGLLRYEGGKGPASSQLPAGPVGW 317

Query: 302 AWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSISKVDGKLRYAINGVSH 361
           AWSLNQFR+FRWNLTASAARPNPQGSYHYG INITRTIKLVN+  KVDGKLR+A+NGVSH
Sbjct: 318 AWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQGKVDGKLRFALNGVSH 377

Query: 362 VDPETPLKLAEYFEVADKVFKYDTISDEGLAEGATTVTVAPNVVNATFRNFIEIIFENHE 421
            +PETPLKLAEYF ++DKVFKYDTI+D+   E    + + PNV+N T R F+E++FENHE
Sbjct: 378 TEPETPLKLAEYFGISDKVFKYDTITDDPTPEQIKNIKIEPNVLNITHRTFVEVVFENHE 437

Query: 422 KSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQVFPKSWAAILLTFDNAG 481
           KS+QSWHLDGYSFF+VA+EPG W+PEKR NYNLLDAVSRHT+QV+PK WAAILLTFDN G
Sbjct: 438 KSVQSWHLDGYSFFSVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKCWAAILLTFDNCG 497

Query: 482 MWNLRSELLENRYLGSQLYVSVLSPARSLRDEYNIPDNTLLCGLVKDMPLP-KPY 536
           MWN+RSE  E RYLG QLY SVLSP +SLRDEYN+P+ +L CGLVK+ P P  PY
Sbjct: 498 MWNVRSENTERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLVKNTPKPVNPY 552

BLAST of Cucsa.152620.1 vs. Swiss-Prot
Match: ASOL_TOBAC (L-ascorbate oxidase homolog OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 815.8 bits (2106), Expect = 2.7e-235
Identity = 376/539 (69.76%), Postives = 450/539 (83.49%), Query Frame = 1

Query: 1   MTATVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNNLVINVFNNL 60
           ++  V  EDPY +F WNVTYGTI+PLGVPQQGILINGQFPGP IN TSNNN+V+NVFNNL
Sbjct: 15  LSVGVIAEDPYLYFNWNVTYGTIAPLGVPQQGILINGQFPGPRINCTSNNNIVVNVFNNL 74

Query: 61  DEPFLLHWSGIQHRKNSWQDGLLGTNCPILPGTNYTYHFQVKDQIGSFFYYPSTAMHRAA 120
           DEPFL  W+G+QHRKNSWQDG  GT CPI+PG N+TY FQVKDQIGS+ Y+P+TA+HRAA
Sbjct: 75  DEPFLFTWNGVQHRKNSWQDGTPGTMCPIMPGQNFTYRFQVKDQIGSYSYFPTTALHRAA 134

Query: 121 GGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRSIARPDGVLING 180
           GG+G L V+SR LIPVP+ +P D+Y V +GDWY K H  LK++LD GR+I RPDG++ING
Sbjct: 135 GGYGALNVHSRALIPVPFDNPADEYNVFVGDWYNKGHKTLKKILDGGRTIGRPDGIIING 194

Query: 181 KSAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMKLVEMEGSHTVQNDYES 240
           KSAK     EPLFTM+ GKTY+YR CN+G++SS+N RFQGH MKLVE+EGSHTVQN Y+S
Sbjct: 195 KSAKVGEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQGHPMKLVELEGSHTVQNIYDS 254

Query: 241 LDVHVGQCFSVLVTANQEPKDYYMVASTRFIKNVLVGKGIVRYIDGKGPASPEIPEAP-- 300
           LD+HVGQC SVLVTA+QEPKDYY+V S+RF+K  L    I+RY +GKGPASPE+P  P  
Sbjct: 255 LDLHVGQCLSVLVTADQEPKDYYLVVSSRFLKQALSSVAIIRYANGKGPASPELPTPPPE 314

Query: 301 --MGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSISKVDGKLRYAI 360
              G AWS+NQFR+FRWNLTASAARPNPQGSYHYG INITRTIK+ NS+S+V GKLRY +
Sbjct: 315 NTEGIAWSMNQFRSFRWNLTASAARPNPQGSYHYGQINITRTIKIFNSMSQVGGKLRYGL 374

Query: 361 NGVSHVDPETPLKLAEYFEVADKVFKYDTISDEGLAEGATTVTVAPNVVNATFRNFIEII 420
           NG+SH + ETPLKL EYF   +K FKYD ++DE  A+  + +T+A NV NAT+RNF+EII
Sbjct: 375 NGISHTNGETPLKLVEYFGATNKAFKYDLMADEAPAD-PSKLTIATNVKNATYRNFVEII 434

Query: 421 FENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQVFPKSWAAILLT 480
           FENHEK+++++HLDGYSFFAVA+EPGRWSPEKR NYNL+D +SR+ IQV+P SWAAI+LT
Sbjct: 435 FENHEKTIRTYHLDGYSFFAVAVEPGRWSPEKRKNYNLVDGLSRNNIQVYPNSWAAIMLT 494

Query: 481 FDNAGMWNLRSELLENRYLGSQLYVSVLSPARSLRDEYNIPDNTLLCGLVKDMPLPKPY 536
           FDNAGMWNLRSE+ E  YLG QLY SVLSP+RSLRDEYNIPDN  LCG+VK + +P PY
Sbjct: 495 FDNAGMWNLRSEMWEKTYLGEQLYFSVLSPSRSLRDEYNIPDNHPLCGIVKGLSMPAPY 552

BLAST of Cucsa.152620.1 vs. Swiss-Prot
Match: SKU5_ARATH (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana GN=SKU5 PE=1 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 2.6e-153
Identity = 270/541 (49.91%), Postives = 363/541 (67.10%), Query Frame = 1

Query: 9   DPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNNLVINVFNNLDEPFLLHW 68
           DPY F+ + V+Y T SPLGVPQQ I ING+FPGP IN T+N NLV+NV N LDE  LLHW
Sbjct: 22  DPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTNENLVVNVRNKLDEGLLLHW 81

Query: 69  SGIQHRKNSWQDGLLGTNCPILPGTNYTYHFQVKDQIGSFFYYPSTAMHRAAGGFGGLRV 128
           +GIQ R+ SWQDG+LGTNCPI P  N+TY FQVKDQIGSFFY+PS    RA+GGFG   V
Sbjct: 82  NGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFFYFPSLHFQRASGGFGSFVV 141

Query: 129 NSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRSIARPDGVLINGK------- 188
           N R +IPVP++ P+ D TV IGDWY ++HTAL++ LD G+ +  PDGVLINGK       
Sbjct: 142 NPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDGKDLGMPDGVLINGKGPYRYND 201

Query: 189 SAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMKLVEMEGSHTVQNDYESL 248
           +   DG D    T+ PGKTY+ RV NVG+ +SLNFR QGH + L E EGS+TVQ +Y SL
Sbjct: 202 TLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHNLVLAESEGSYTVQQNYTSL 261

Query: 249 DVHVGQCFSVLVTANQE-PKDYYMVASTRFIKNVL----VGKGIVRYIDGKGPASPEIPE 308
           D+HVGQ +S LVT +Q    DYY+VAS R +   +     G GI++Y + KG A  ++P 
Sbjct: 262 DIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRVTGVGILKYTNSKGKAKGQLPP 321

Query: 309 AP---MGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSIS-KVDGKL 368
            P       +S+NQ R+ RWN++AS ARPNPQGS+ YGSIN+T    L N     + GK 
Sbjct: 322 GPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGSINVTDVYVLRNMPPVTISGKR 381

Query: 369 RYAINGVSHVDPETPLKLAEYFEVADKVFKYDTISDEGLAEGATTVTVAPNVVNATFRNF 428
           R  +NG+S  +P TP++LA+  +V D V+K D      L   A    VA +++N T+R F
Sbjct: 382 RTTLNGISFKNPSTPIRLADKLKVKD-VYKLD-FPKRPLTGPA---KVATSIINGTYRGF 441

Query: 429 IEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQVFPKSWAA 488
           +E++ +N++  +QS+H+ GY+FF V ++ G W+   R  YN  D ++R TIQV+P +W+A
Sbjct: 442 MEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGTYNKWDGIARSTIQVYPGAWSA 501

Query: 489 ILLTFDNAGMWNLRSELLENRYLGSQLYVSVLSPARSLRDEYNIPDNTLLCGLVKDMPLP 534
           IL++ DN G WNLR+E L++ YLG + YV V++P  + + E+  PDN L CG +  +  P
Sbjct: 502 ILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNKTEFGHPDNVLYCGALSKLQKP 557

BLAST of Cucsa.152620.1 vs. Swiss-Prot
Match: SKS2_ARATH (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana GN=SKS2 PE=1 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 1.4e-146
Identity = 255/539 (47.31%), Postives = 373/539 (69.20%), Query Frame = 1

Query: 9   DPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNNLVINVFNNLDEPFLLHW 68
           DPY  + + ++Y T SPLGVPQQ I +NG+FPGP IN+T+N N+ +NV N+LDEP LL W
Sbjct: 25  DPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVINATTNYNVHVNVLNHLDEPLLLTW 84

Query: 69  SGIQHRKNSWQDGLLGTNCPILPGTNYTYHFQVKDQIGSFFYYPSTAMHRAAGGFGGLRV 128
            G+Q R+NSWQDG+LGTNCPI P  N+TY FQ+KDQIGS+FY PS    RA+GGFG L +
Sbjct: 85  PGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKDQIGSYFYSPSLNFQRASGGFGALII 144

Query: 129 NSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRSIARPDGVLINGK------S 188
           N+R L+P+P+ +P+ +   +IGDWYT++HTAL+++LDSG+ +  PDGVLINGK      S
Sbjct: 145 NNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRILDSGKELGMPDGVLINGKGPFKYNS 204

Query: 189 AKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMKLVEMEGSHTVQNDYESLD 248
           +  DG +     + PGKTY+ RV NVG+ +SLNFR Q H + L+E EG +T Q ++   D
Sbjct: 205 SVPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFRIQNHKLLLIETEGRYTSQMNFTDFD 264

Query: 249 VHVGQCFSVLVTANQE-PKDYYMVASTRFIKNV----LVGKGIVRYIDGKGPASPEIP-- 308
           VHVGQ +S LVT +Q    DYY+VAS RF+       + G GI+ Y + KGPAS  +P  
Sbjct: 265 VHVGQSYSFLVTMDQNATSDYYIVASARFVNETVWQRVTGVGILHYSNSKGPASGPLPVS 324

Query: 309 EAPMGWAWS-LNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSI--SKVDGKL 368
              +   WS +NQ R  + N +AS ARPNPQGS+HYG INITRT  ++ S+  +K++GKL
Sbjct: 325 ATDVNHPWSAMNQPRAIKQNTSASGARPNPQGSFHYGQINITRTY-ILRSLPPTKINGKL 384

Query: 369 RYAINGVSHVDPETPLKLAEYFEV-ADKVFKY-DTISDEGLAEGATTVTVAPNVVNATFR 428
           R  +NG+S V+P TP++LA+  +V  D +  + D   DE L        ++ +++NAT++
Sbjct: 385 RATLNGISFVNPSTPMRLADDHKVKGDYMLDFPDRPLDEKLPR------LSSSIINATYK 444

Query: 429 NFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQVFPKSW 488
            FI++IF+N++  +QS+H+DGY+F+ VA++ G WS ++ ++YN  DAV+R T++V+P +W
Sbjct: 445 GFIQVIFQNNDTKIQSFHIDGYAFYVVAMDFGIWSEDRNSSYNNWDAVARSTVEVYPGAW 504

Query: 489 AAILLTFDNAGMWNLRSELLENRYLGSQLYVSVLSPARSLRDEYNIPDNTLLCGLVKDM 530
            A+L++ DN G+WN+R E L+  YLG + Y+ +++P  +   E + P+N + CG ++ M
Sbjct: 505 TAVLISLDNVGVWNIRVENLDRWYLGQETYMRIINPEENGSTEMDPPENVMYCGALQAM 556

BLAST of Cucsa.152620.1 vs. Swiss-Prot
Match: SKS1_ARATH (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana GN=SKS1 PE=1 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 2.0e-145
Identity = 259/545 (47.52%), Postives = 370/545 (67.89%), Query Frame = 1

Query: 1   MTATVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNNLVINVFNNL 60
           ++A     DP+  + + V+Y T SPLGVPQQ I +NGQFPGP +N+T+N N+V+NVFN+L
Sbjct: 18  LSAVSFAADPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPLLNATTNYNVVVNVFNHL 77

Query: 61  DEPFLLHWSGIQHRKNSWQDGLLGTNCPILPGTNYTYHFQVKDQIGSFFYYPSTAMHRAA 120
           DEP LL W GIQ R+NSWQDG+LGTNCPI P  N+TY FQVKDQIGSFFY PS    RA+
Sbjct: 78  DEPLLLTWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQVKDQIGSFFYSPSLNFQRAS 137

Query: 121 GGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRSIARPDGVLING 180
           GGFG + +N+R +IP+P+  P+ +   +IGDWYT+ H AL++ LDSG+ +  PDGVLING
Sbjct: 138 GGFGPIVINNRDIIPIPFPQPDGELIFIIGDWYTQDHKALRRALDSGKELGMPDGVLING 197

Query: 181 K------SAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMKLVEMEGSHTV 240
           K      S+  DG D   F ++PGKTY+ RV NVG+ +SLNFR Q H++ LVE EG +T 
Sbjct: 198 KGPYKYNSSVPDGIDYLTFHVEPGKTYRIRVHNVGISTSLNFRIQNHSLLLVETEGHYTS 257

Query: 241 QNDYESLDVHVGQCFSVLVTANQE-PKDYYMVASTRFIKNV----LVGKGIVRYIDGKGP 300
           Q ++   DVHVGQ +S LVT +Q+   DYY+VAS RF+       + G  I+ Y + KGP
Sbjct: 258 QANFTDFDVHVGQSYSFLVTMDQDATSDYYIVASARFVNETVWQRVTGVAILHYSNSKGP 317

Query: 301 ASP--EIPEAPMGWAWS-LNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSI- 360
            S    +P+  +   WS ++Q +T R N +AS ARPNPQGS+HYG INIT T  ++ S+ 
Sbjct: 318 VSGPLPVPKTDVSSPWSAMSQPKTIRQNTSASGARPNPQGSFHYGQINITNTY-ILRSLP 377

Query: 361 -SKVDGKLRYAINGVSHVDPETPLKLAEYFEVADKVFKYDTISDEGLAEGATTVTVAPNV 420
            + ++G LR  +NG+S V+P TP++LA+  +V    +K D   D         + +  ++
Sbjct: 378 PTIINGALRATLNGISFVNPSTPVRLADRNKVKG-AYKLD-FPDRPFNR---PLRLDRSM 437

Query: 421 VNATFRNFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQ 480
           +NAT++ FI+++F+N++  +QS+H+DGYSFF V ++ G WS +K+ +YN  DA+SR TI+
Sbjct: 438 INATYKGFIQVVFQNNDTKIQSFHVDGYSFFVVGMDFGIWSEDKKGSYNNWDAISRSTIE 497

Query: 481 VFPKSWAAILLTFDNAGMWNLRSELLENRYLGSQLYVSVLSPARSLRDEYNIPDNTLLCG 530
           V+P  W A+L++ DN G+WN+R E L+  YLG + Y+ + +P    + E + PDN L CG
Sbjct: 498 VYPGGWTAVLISLDNVGVWNIRVENLDRWYLGEETYMRITNPEEDGKTEMDPPDNVLYCG 556

BLAST of Cucsa.152620.1 vs. TrEMBL
Match: M5W850_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003715mg PE=4 SV=1)

HSP 1 Score: 959.1 bits (2478), Expect = 2.2e-276
Identity = 450/534 (84.27%), Postives = 489/534 (91.57%), Query Frame = 1

Query: 3   ATVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNNLVINVFNNLDE 62
           + V+GEDPY +FTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNN+V+NVFNNLDE
Sbjct: 18  SVVQGEDPYLYFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNNIVLNVFNNLDE 77

Query: 63  PFLLHWSGIQHRKNSWQDGLLGTNCPILPGTNYTYHFQVKDQIGSFFYYPSTAMHRAAGG 122
           P L  WSG+Q RKNSWQDG LGT CPI PG NYTY FQVKDQIGS+FYYP TAMHRA+GG
Sbjct: 78  PLLFTWSGVQQRKNSWQDGTLGTMCPIPPGQNYTYRFQVKDQIGSYFYYPITAMHRASGG 137

Query: 123 FGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRSIARPDGVLINGKS 182
           FGGLR+NSRLLIPVPYADPEDDYTVLIGDWY K HTALK+ LDSGRS+ARPDGV+INGKS
Sbjct: 138 FGGLRINSRLLIPVPYADPEDDYTVLIGDWYAKGHTALKKHLDSGRSMARPDGVIINGKS 197

Query: 183 AKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMKLVEMEGSHTVQNDYESLD 242
           AKGDG DEPLFTMKPGKTYKYR+CNVGLK+SLNFR QGH MKLVEMEGSHTVQN YESLD
Sbjct: 198 AKGDGKDEPLFTMKPGKTYKYRICNVGLKNSLNFRIQGHPMKLVEMEGSHTVQNTYESLD 257

Query: 243 VHVGQCFSVLVTANQEPKDYYMVASTRFIKNVLVGKGIVRYIDGKGPASPEIPEAPMGWA 302
           VHVGQCFSVLVTA+++PKDYYMVASTRF K VL GKGI+RY +G GPASPE+PEAP+GWA
Sbjct: 258 VHVGQCFSVLVTADKDPKDYYMVASTRFTKKVLTGKGIMRYENGNGPASPELPEAPVGWA 317

Query: 303 WSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSISKVDGKLRYAINGVSHV 362
           WSLNQFR+FRWNLTASAARPNPQGSYHYG INITRT+K+VNS +KVDGKLRYAINGVSHV
Sbjct: 318 WSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTLKIVNSATKVDGKLRYAINGVSHV 377

Query: 363 DPETPLKLAEYFEVADKVFKYDTISDEGLAEGATTVTVAPNVVNATFRNFIEIIFENHEK 422
           DPETPLKLAEY+ VADKVFKYDTI D+  A     VT+APNVVN TFRNF+EIIFENHEK
Sbjct: 378 DPETPLKLAEYYGVADKVFKYDTIQDDPPATVEDKVTLAPNVVNQTFRNFVEIIFENHEK 437

Query: 423 SLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQVFPKSWAAILLTFDNAGM 482
           S+QSW+LDGYSFFAVAIEPGRW+PEKR NYNLLD VSRHTIQVFPKSWAAI+LTFDNAGM
Sbjct: 438 SIQSWNLDGYSFFAVAIEPGRWAPEKRKNYNLLDGVSRHTIQVFPKSWAAIMLTFDNAGM 497

Query: 483 WNLRSELLENRYLGSQLYVSVLSPARSLRDEYNIPDNTLLCGLVKDMPLPKPYT 537
           WN+RSE  E RYLG Q YVSVLSPARSLRDEYN+PDNTL+CG+VKD+P P PY+
Sbjct: 498 WNVRSEQSERRYLGQQFYVSVLSPARSLRDEYNLPDNTLVCGIVKDLPKPPPYS 551

BLAST of Cucsa.152620.1 vs. TrEMBL
Match: B9GXW4_POPTR (Multi-copper oxidase type 1 family protein OS=Populus trichocarpa GN=POPTR_0003s20680g PE=4 SV=1)

HSP 1 Score: 948.0 bits (2449), Expect = 5.1e-273
Identity = 443/534 (82.96%), Postives = 489/534 (91.57%), Query Frame = 1

Query: 5   VRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNNLVINVFNNLDEPF 64
           VRGEDPY FFTWNVTYGT+SPLGVPQQ ILIN QFPGPNINSTSNNN+VINVFNNLDEPF
Sbjct: 20  VRGEDPYLFFTWNVTYGTLSPLGVPQQVILINNQFPGPNINSTSNNNIVINVFNNLDEPF 79

Query: 65  LLHWSGIQHRKNSWQDGLLGTNCPILPGTNYTYHFQVKDQIGSFFYYPSTAMHRAAGGFG 124
           LL WSGIQ RKNSWQDG+LGTNCPILPGTNYTYHFQVKDQIGS+ YYP+T MHRA GGFG
Sbjct: 80  LLTWSGIQQRKNSWQDGVLGTNCPILPGTNYTYHFQVKDQIGSYIYYPTTGMHRATGGFG 139

Query: 125 GLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRSIARPDGVLINGKSAK 184
           GLR+NSRLLIPVPYADPEDDYTV++ DWY+KSHTAL++LLDSGR++ARPDGVL+NGK+AK
Sbjct: 140 GLRINSRLLIPVPYADPEDDYTVILNDWYSKSHTALQKLLDSGRTLARPDGVLVNGKNAK 199

Query: 185 GDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMKLVEMEGSHTVQNDYESLDVH 244
           GDG DEPLFTMKPGKTYKYR+CN GLK++LNFR QGHTMKLVEMEGSH VQN YESLDVH
Sbjct: 200 GDGKDEPLFTMKPGKTYKYRICNAGLKNTLNFRIQGHTMKLVEMEGSHVVQNVYESLDVH 259

Query: 245 VGQCFSVLVTANQEPKDYYMVASTRFIKNVLVGKGIVRYIDGKGPASPEIPEAPMGWAWS 304
           VGQCFSVLVTANQ+PKDYYMVASTRF K VL GKGI+RY +GKGPASPE+PEAP+GWAWS
Sbjct: 260 VGQCFSVLVTANQDPKDYYMVASTRFNKQVLTGKGIIRYTNGKGPASPELPEAPVGWAWS 319

Query: 305 LNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSISKVDGKLRYAINGVSHVDP 364
           LNQFR+FRWNLTASAARPNPQGSYHYGSINITRTIKL+NS S+  GKLRYA+NGVSHV+P
Sbjct: 320 LNQFRSFRWNLTASAARPNPQGSYHYGSINITRTIKLINSASQEGGKLRYALNGVSHVNP 379

Query: 365 ETPLKLAEYFEVADKVFKYDTISDEGLAEGATT-VTVAPNVVNATFRNFIEIIFENHEKS 424
           ETPLKLAEY+ VADKVFKYDTI D   A  A   +   PNV+N TFRNF+EIIFENHEKS
Sbjct: 380 ETPLKLAEYYGVADKVFKYDTILDSPQANVANAKIVTQPNVLNLTFRNFVEIIFENHEKS 439

Query: 425 LQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQVFPKSWAAILLTFDNAGMW 484
           +QS+HLDGYSFFAVA+EPG W+PEKR +YNLLDAVSR T+QV+PKSWAAILLTFDNAG+W
Sbjct: 440 MQSYHLDGYSFFAVAVEPGTWTPEKRKHYNLLDAVSRTTVQVYPKSWAAILLTFDNAGLW 499

Query: 485 NLRSELLENRYLGSQLYVSVLSPARSLRDEYNIPDNTLLCGLVKDMPLPKPYTI 538
           N+RSE+ E  YLG QLY SVLSPARSLRDEYN+PDN+LLCG+VKDMP P PY+I
Sbjct: 500 NIRSEMWERAYLGQQLYASVLSPARSLRDEYNLPDNSLLCGIVKDMPKPAPYSI 553

BLAST of Cucsa.152620.1 vs. TrEMBL
Match: B9GLA5_POPTR (Multi-copper oxidase type 1 family protein OS=Populus trichocarpa GN=POPTR_0001s03770g PE=4 SV=2)

HSP 1 Score: 941.4 bits (2432), Expect = 4.8e-271
Identity = 444/534 (83.15%), Postives = 488/534 (91.39%), Query Frame = 1

Query: 5   VRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNNLVINVFNNLDEPF 64
           V+GEDPY FFTWNVTYGT+SPLGVPQQ ILIN QFPGPNINSTSNNN+VINVFNN+DEPF
Sbjct: 20  VQGEDPYLFFTWNVTYGTLSPLGVPQQVILINDQFPGPNINSTSNNNIVINVFNNIDEPF 79

Query: 65  LLHWSGIQHRKNSWQDGLLGTNCPILPGTNYTYHFQVKDQIGSFFYYPSTAMHRAAGGFG 124
           LL WSGIQ RKNSWQDG+LGTNCPILPGTN+TYHFQVKDQIGS+ YYP+T MHRAAGGFG
Sbjct: 80  LLTWSGIQQRKNSWQDGVLGTNCPILPGTNFTYHFQVKDQIGSYIYYPTTGMHRAAGGFG 139

Query: 125 GLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRSIARPDGVLINGKSAK 184
           GLR+NSRLLIPVPYADPEDDYTV++ DWYTKSHTAL++LLDSGRS+ARPDGVLINGK+A 
Sbjct: 140 GLRINSRLLIPVPYADPEDDYTVVLNDWYTKSHTALQKLLDSGRSLARPDGVLINGKNAM 199

Query: 185 GDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMKLVEMEGSHTVQNDYESLDVH 244
           GDG DEPLFTMKP KTYKYR+CN GLK++LNFR Q HTMKLVEMEGSH VQN YESLDVH
Sbjct: 200 GDGKDEPLFTMKPEKTYKYRICNAGLKNTLNFRIQDHTMKLVEMEGSHVVQNVYESLDVH 259

Query: 245 VGQCFSVLVTANQEPKDYYMVASTRFIKNVLVGKGIVRYIDGKGPASPEIPEAPMGWAWS 304
           VGQCFSVLVTANQ PKDYYMVASTRF K VL  KGI+RY +GKGPASPE+PEAP+GWAWS
Sbjct: 260 VGQCFSVLVTANQAPKDYYMVASTRFNKQVLTAKGIIRYTNGKGPASPELPEAPVGWAWS 319

Query: 305 LNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSISKVDGKLRYAINGVSHVDP 364
           LNQFR+FRWNLTASAARPNPQGSYHYGSINITRTIKLVNS S+  GKLRYA+NGVSHVDP
Sbjct: 320 LNQFRSFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSASREGGKLRYALNGVSHVDP 379

Query: 365 ETPLKLAEYFEVADKVFKYDTISDEGLAEGATTVTVA-PNVVNATFRNFIEIIFENHEKS 424
           ETPLK+AEY+ VADK+FKYDT+ D   A+ A    V+ PNV+N TFRNF+EIIFENHEKS
Sbjct: 380 ETPLKIAEYYGVADKLFKYDTMQDSPQADIAHAKIVSQPNVLNLTFRNFVEIIFENHEKS 439

Query: 425 LQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQVFPKSWAAILLTFDNAGMW 484
           +QS+HLDGYSFFAVA+EPG W+PEKR +YNLLDAVSR T+QVFPKSWAAILLTFDNAGMW
Sbjct: 440 MQSYHLDGYSFFAVAVEPGTWTPEKRKHYNLLDAVSRTTVQVFPKSWAAILLTFDNAGMW 499

Query: 485 NLRSELLENRYLGSQLYVSVLSPARSLRDEYNIPDNTLLCGLVKDMPLPKPYTI 538
           N+RSE+ E  YLG QLY SVLSPARSLRDEYNIPDNTLLCGLVKD+P P+PY+I
Sbjct: 500 NIRSEMWERAYLGQQLYASVLSPARSLRDEYNIPDNTLLCGLVKDLPKPEPYSI 553

BLAST of Cucsa.152620.1 vs. TrEMBL
Match: W9QSQ9_9ROSA (L-ascorbate oxidase-like protein OS=Morus notabilis GN=L484_005159 PE=4 SV=1)

HSP 1 Score: 936.0 bits (2418), Expect = 2.0e-269
Identity = 439/533 (82.36%), Postives = 483/533 (90.62%), Query Frame = 1

Query: 6   RGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNNLVINVFNNLDEPFL 65
           R EDPY FFTWNVTYGTISPLGVPQQGILINGQFPGPNINST+NNN+V+NVFNN+DEP L
Sbjct: 3   RAEDPYLFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTTNNNIVLNVFNNIDEPIL 62

Query: 66  LHWSGIQHRKNSWQDGLLGTNCPILPGTNYTYHFQVKDQIGSFFYYPSTAMHRAAGGFGG 125
             W GIQ RKNSWQDG+LGTNCPILPGTN+TY FQVKDQIGS+FYYP+TA+HRAAGGFGG
Sbjct: 63  FTWLGIQQRKNSWQDGVLGTNCPILPGTNFTYRFQVKDQIGSYFYYPTTAIHRAAGGFGG 122

Query: 126 LRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRSIARPDGVLINGKSAKG 185
           LRVNSRLLIPVPYADPEDDYT+LIGDWYT+SH+ L++ LD GRS+ RPDGVLINGKSAK 
Sbjct: 123 LRVNSRLLIPVPYADPEDDYTILIGDWYTESHSTLRKFLDIGRSLGRPDGVLINGKSAKS 182

Query: 186 DGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMKLVEMEGSHTVQNDYESLDVHV 245
           DG+DEPLFTMKPGK YKYR+CNVGLK+SLNFR QGH +KLVEMEGSHTVQN YESLDVHV
Sbjct: 183 DGSDEPLFTMKPGKIYKYRICNVGLKNSLNFRIQGHPLKLVEMEGSHTVQNTYESLDVHV 242

Query: 246 GQCFSVLVTANQEPKDYYMVASTRFIKNVLVGKGIVRYIDGK-GPASPEIPEAPMGWAWS 305
           GQCF+VLVTA++ PKDY +VASTRF KNVL GKGI+RY + K  P SP++ EAP+GWAWS
Sbjct: 243 GQCFAVLVTADKAPKDYLVVASTRFTKNVLTGKGIIRYTNAKPAPPSPDVVEAPVGWAWS 302

Query: 306 LNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSISKVDGKLRYAINGVSHVDP 365
           LNQFR+FRWNLT+SAARPNPQGSYHYG INITRTIKLVNS  +V GKLRYAINGVSHVDP
Sbjct: 303 LNQFRSFRWNLTSSAARPNPQGSYHYGKINITRTIKLVNSAVRVQGKLRYAINGVSHVDP 362

Query: 366 ETPLKLAEYFEVADKVFKYDTISDEGLAEGATTVTVAPNVVNATFRNFIEIIFENHEKSL 425
            TPLKLAEY++VADKVFKYD ISDE  A     +TVA NVVN TFRNF+EIIFENHEKSL
Sbjct: 363 YTPLKLAEYYQVADKVFKYDIISDEPPANAGDKITVATNVVNQTFRNFVEIIFENHEKSL 422

Query: 426 QSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQVFPKSWAAILLTFDNAGMWN 485
           Q+WHLDGYSFFAVAIEPGRWSP+KR+ YNLLDAVSRHTIQVFPKSWAAI+LTFDNAGMWN
Sbjct: 423 QTWHLDGYSFFAVAIEPGRWSPDKRSRYNLLDAVSRHTIQVFPKSWAAIMLTFDNAGMWN 482

Query: 486 LRSELLENRYLGSQLYVSVLSPARSLRDEYNIPDNTLLCGLVKDMPLPKPYTI 538
           LRSEL E RYLG QLY+SV SPARSLRDEYN+PDN LLCG+VKD+P P PY+I
Sbjct: 483 LRSELTERRYLGQQLYISVQSPARSLRDEYNMPDNALLCGVVKDLPWPPPYSI 535

BLAST of Cucsa.152620.1 vs. TrEMBL
Match: A0A061GSZ7_THECC (SKU5 similar 13 OS=Theobroma cacao GN=TCM_039977 PE=4 SV=1)

HSP 1 Score: 931.4 bits (2406), Expect = 4.9e-268
Identity = 444/534 (83.15%), Postives = 481/534 (90.07%), Query Frame = 1

Query: 5   VRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNNLVINVFNNLDEPF 64
           VRGEDPY FFTWNVTYGTISPLGVPQQ ILINGQFPGPNINST+NNN+VINVFNNLDEPF
Sbjct: 21  VRGEDPYLFFTWNVTYGTISPLGVPQQAILINGQFPGPNINSTTNNNIVINVFNNLDEPF 80

Query: 65  LLHWSGIQHRKNSWQDGLLGTNCPILPGTNYTYHFQVKDQIGSFFYYPSTAMHRAAGGFG 124
           LL WSGIQHRKNSWQDG+LGTNCPI PG NYTYHFQVKDQIGS+ YYPSTAMHRAAGGFG
Sbjct: 81  LLTWSGIQHRKNSWQDGVLGTNCPIPPGKNYTYHFQVKDQIGSYIYYPSTAMHRAAGGFG 140

Query: 125 GLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRSIARPDGVLINGKSAK 184
           GLRVNSRLLIPVPY+DPE DYTVLIGDWYTKSHT LK+ LDSGRS+ RPDGVLINGK AK
Sbjct: 141 GLRVNSRLLIPVPYSDPEADYTVLIGDWYTKSHTMLKKFLDSGRSLGRPDGVLINGKVAK 200

Query: 185 GDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMKLVEMEGSHTVQNDYESLDVH 244
           G+G D+PLFTMKPGKTYKYR+CNVGLK+SLNFR QGH MKLVEMEGSHTVQN YESLDVH
Sbjct: 201 GNGKDKPLFTMKPGKTYKYRICNVGLKNSLNFRIQGHRMKLVEMEGSHTVQNVYESLDVH 260

Query: 245 VGQCFSVLVTANQEPKDYYMVASTRFIKNVLVGKGIVRYIDGKG-PASPEIPEAPMGWAW 304
           VGQCF+VLVTA+Q PKDY+MVASTRF KNVL   GI+RY +GKG  ASP++P AP+GWAW
Sbjct: 261 VGQCFAVLVTADQGPKDYFMVASTRFTKNVLTATGIIRYTNGKGAAASPKLPPAPVGWAW 320

Query: 305 SLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSISKVDGKLRYAINGVSHVD 364
           SLNQFRTFRWNLTASAARPNPQGSYHYG+INITRTIKLVN+ S+  GKLRY INGVSH++
Sbjct: 321 SLNQFRTFRWNLTASAARPNPQGSYHYGAINITRTIKLVNTASRAGGKLRYGINGVSHIN 380

Query: 365 PETPLKLAEYFEVADKVFKYDTISDEGLAEGATTVTVAPNVVNATFRNFIEIIFENHEKS 424
           P TPLKLAEY+ VA+KVFKYD I DE  A+  T VTV PNV+N TFRNF+EII ENHEKS
Sbjct: 381 PPTPLKLAEYYNVANKVFKYDVIGDEPPAK-ITGVTVQPNVLNMTFRNFVEIILENHEKS 440

Query: 425 LQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQVFPKSWAAILLTFDNAGMW 484
           +QS+HLDGYSFFAVAIEPGRW+PEKR NYNLLDAVSRHTIQVFPKSWAAILLTFDNAGMW
Sbjct: 441 IQSYHLDGYSFFAVAIEPGRWTPEKRRNYNLLDAVSRHTIQVFPKSWAAILLTFDNAGMW 500

Query: 485 NLRSELLENRYLGSQLYVSVLSPARSLRDEYNIPDNTLLCGLVKDMPLPKPYTI 538
           N+RSE+ E  YLG QLY SV SP RSLRDEYNIPDN  LCG+V ++P P PYTI
Sbjct: 501 NIRSEIWERTYLGQQLYASVASPVRSLRDEYNIPDNAPLCGVVTNLPKPPPYTI 553

BLAST of Cucsa.152620.1 vs. TAIR10
Match: AT3G13390.1 (AT3G13390.1 SKU5 similar 11)

HSP 1 Score: 901.7 bits (2329), Expect = 2.1e-262
Identity = 414/534 (77.53%), Postives = 467/534 (87.45%), Query Frame = 1

Query: 2   TATVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNNLVINVFNNLD 61
           T  VR EDPYF   WNVTYGT+SPLGVPQQ ILINGQFPGPN+NSTSNNN++INVFNNLD
Sbjct: 18  TVVVRAEDPYFHHVWNVTYGTVSPLGVPQQVILINGQFPGPNVNSTSNNNVIINVFNNLD 77

Query: 62  EPFLLHWSGIQHRKNSWQDGLLGTNCPILPGTNYTYHFQVKDQIGSFFYYPSTAMHRAAG 121
           EPFLL W+GIQHRKN WQDG  GT CPI+PGTNYTYHFQ KDQIGS+FYYPSTAMHR+AG
Sbjct: 78  EPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIGSYFYYPSTAMHRSAG 137

Query: 122 GFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRSIARPDGVLINGK 181
           GFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHT LK+ LDSGR++ RPDG+LINGK
Sbjct: 138 GFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDSGRTLGRPDGILINGK 197

Query: 182 SAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMKLVEMEGSHTVQNDYESL 241
           S KGDG+D PLFT+KPGKTY+ R+CNVGLK+SLNFR Q H +KLVEMEGSH +QNDY+SL
Sbjct: 198 SGKGDGSDAPLFTLKPGKTYRVRICNVGLKTSLNFRIQNHKLKLVEMEGSHVLQNDYDSL 257

Query: 242 DVHVGQCFSVLVTANQEPKDYYMVASTRFIKNVLVGKGIVRYIDGKGPASPEIPEAPMGW 301
           DVHVGQC+  ++TANQE KDYYMVAS+RF+K+V+   G++RY  GKGPAS ++P  P+GW
Sbjct: 258 DVHVGQCYGTILTANQEAKDYYMVASSRFLKSVITTTGLLRYEGGKGPASSQLPPGPVGW 317

Query: 302 AWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSISKVDGKLRYAINGVSH 361
           AWSLNQFR+FRWNLTASAARPNPQGSYHYG INITRTIKLVN+  KVDGKLRYA+NGVSH
Sbjct: 318 AWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQGKVDGKLRYALNGVSH 377

Query: 362 VDPETPLKLAEYFEVADKVFKYDTISDEGLAEGATTVTVAPNVVNATFRNFIEIIFENHE 421
            DPETPLKLAEYF VADKVFKYD+I+D    E   ++ + PNV+N T R FIE++FENHE
Sbjct: 378 TDPETPLKLAEYFGVADKVFKYDSITDNPTPEQIKSIKIVPNVLNITHRTFIEVVFENHE 437

Query: 422 KSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQVFPKSWAAILLTFDNAG 481
           KS+QSWHLDGYSFFAVA+EPG W+PEKR NYNLLDAVSRHT+QV+PK WAAILLTFDN G
Sbjct: 438 KSVQSWHLDGYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKCWAAILLTFDNCG 497

Query: 482 MWNLRSELLENRYLGSQLYVSVLSPARSLRDEYNIPDNTLLCGLVKDMPLPKPY 536
           MWN+RSE  E RYLG QLY SVLSP +SLRDEYN+P+ +L CGLVK  P P PY
Sbjct: 498 MWNVRSENSERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLVKGTPKPNPY 551

BLAST of Cucsa.152620.1 vs. TAIR10
Match: AT1G55570.1 (AT1G55570.1 SKU5 similar 12)

HSP 1 Score: 886.7 bits (2290), Expect = 7.1e-258
Identity = 409/531 (77.02%), Postives = 461/531 (86.82%), Query Frame = 1

Query: 5   VRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNNLVINVFNNLDEPF 64
           V+ EDPYF   WNVTYGT SPLGVPQQ ILINGQFPGPNINSTSNNN+++NVFNNLDEPF
Sbjct: 22  VQAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINSTSNNNVIVNVFNNLDEPF 81

Query: 65  LLHWSGIQHRKNSWQDGLLGTNCPILPGTNYTYHFQVKDQIGSFFYYPSTAMHRAAGGFG 124
           L+ W+GIQHRKN WQDG  GT CPI PG N+TYHFQ KDQIGS+FYYP+TAMHRAAGGFG
Sbjct: 82  LITWAGIQHRKNCWQDGTAGTMCPIPPGQNFTYHFQPKDQIGSYFYYPTTAMHRAAGGFG 141

Query: 125 GLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRSIARPDGVLINGKSAK 184
           GLRVNSRLLIPVPYADPEDDYT+LI DWYTKSHT LK+ LDSGR+I RPDG+LINGKS K
Sbjct: 142 GLRVNSRLLIPVPYADPEDDYTILINDWYTKSHTQLKKFLDSGRTIGRPDGILINGKSGK 201

Query: 185 GDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMKLVEMEGSHTVQNDYESLDVH 244
            DG+D+PLFT+KPGKTY+ R+CNVGLK+SLNFR Q H MKLVEMEGSH +QNDY+SLDVH
Sbjct: 202 TDGSDKPLFTLKPGKTYRVRICNVGLKASLNFRIQNHKMKLVEMEGSHVLQNDYDSLDVH 261

Query: 245 VGQCFSVLVTANQEPKDYYMVASTRFIKNVLVGKGIVRYIDGKGPASPEIPEAPMGWAWS 304
           VGQCF V+VTA+QEPKDYYM+ASTRF+K  L   G++RY  GKGPAS ++P AP+GWAWS
Sbjct: 262 VGQCFGVIVTADQEPKDYYMIASTRFLKKPLTTTGLLRYEGGKGPASSQLPAAPVGWAWS 321

Query: 305 LNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSISKVDGKLRYAINGVSHVDP 364
           LNQ+R+FRWNLTASAARPNPQGSYHYG INITRTIKLVN+  KVDGKLRYA++GVSH DP
Sbjct: 322 LNQYRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQGKVDGKLRYALSGVSHTDP 381

Query: 365 ETPLKLAEYFEVADKVFKYDTISDEGLAEGATTVTVAPNVVNATFRNFIEIIFENHEKSL 424
           ETPLKLAEYF VADKVFKYDTISD    +    + + PNV+N T R FIE++FENHE+S+
Sbjct: 382 ETPLKLAEYFGVADKVFKYDTISDNPNPDQIKNIKIEPNVLNITHRTFIEVVFENHERSV 441

Query: 425 QSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQVFPKSWAAILLTFDNAGMWN 484
           QSWHLDGYSFFAVA+EPG W+PEKR NYNLLDAVSRHT+QV+PK WAAILLTFDN GMWN
Sbjct: 442 QSWHLDGYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKCWAAILLTFDNCGMWN 501

Query: 485 LRSELLENRYLGSQLYVSVLSPARSLRDEYNIPDNTLLCGLVKDMPLPKPY 536
           +RSE  E RYLG QLY SVLSP +SLRDEYN+P+ +L CGLVK  P   PY
Sbjct: 502 IRSENAERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLVKGKPKVNPY 552

BLAST of Cucsa.152620.1 vs. TAIR10
Match: AT3G13400.1 (AT3G13400.1 SKU5 similar 13)

HSP 1 Score: 866.3 bits (2237), Expect = 9.9e-252
Identity = 408/535 (76.26%), Postives = 464/535 (86.73%), Query Frame = 1

Query: 3   ATVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNNLVINVFNNLDE 62
           A V   DPYF++TWNVTYGT +PLG+PQQ ILINGQFPGPN+NSTSNNN+VINVFNNLDE
Sbjct: 18  ALVSAGDPYFYYTWNVTYGTAAPLGIPQQVILINGQFPGPNLNSTSNNNVVINVFNNLDE 77

Query: 63  PFLLHWSGIQHRKNSWQDGLLGTNCPILPGTNYTYHFQVKDQIGSFFYYPSTAMHRAAGG 122
           PFLL WSG+QHRKNSWQDG+ GT+CPI  GTN+TYHFQ KDQIGS+FYYPSTA+HR AGG
Sbjct: 78  PFLLTWSGLQHRKNSWQDGVTGTSCPIPAGTNFTYHFQPKDQIGSYFYYPSTALHRFAGG 137

Query: 123 FGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRSIARPDGVLINGKS 182
           FGGLRVNSRLLIPVPYADPEDD T+LI DWY KSHTALK  LDSGR++  PDGVLINGKS
Sbjct: 138 FGGLRVNSRLLIPVPYADPEDDRTILINDWYAKSHTALKNFLDSGRTLGSPDGVLINGKS 197

Query: 183 AKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMKLVEMEGSHTVQNDYESLD 242
            K  G + PLFTMKPGKTYKYR+CNVG KS+LNFR QGH MKLVEMEGSH +QNDY+SLD
Sbjct: 198 GKLGGNNAPLFTMKPGKTYKYRICNVGFKSTLNFRIQGHKMKLVEMEGSHVLQNDYDSLD 257

Query: 243 VHVGQCFSVLVTANQEPKDYYMVASTRFIKNVLVGKGIVRYIDGKGPASPEIPEAPMGWA 302
           VHVGQCF+VLVTA+Q  K+YYMVASTRF+K  +   G++ Y      AS +IP+AP+GWA
Sbjct: 258 VHVGQCFAVLVTADQVAKNYYMVASTRFLKKEVSTVGVMSYEGSNVQASSDIPKAPVGWA 317

Query: 303 WSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSISKVDGKLRYAINGVSHV 362
           WSLNQFR+FRWNLTASAARPNPQGSYHYG INITRTIKL N+ + V+GK+R+  NGVSHV
Sbjct: 318 WSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLANTKNLVNGKVRFGFNGVSHV 377

Query: 363 DPETPLKLAEYFEVADKVFKYDTISDEGLAEGATTVTVAPNVVNATFRNFIEIIFENHEK 422
           D ETPLKLAEYF +++KVFKY+ I DE  A+  TT+TV PNV+N TFR F+E++FENHEK
Sbjct: 378 DTETPLKLAEYFGMSEKVFKYNVIKDEPAAK-ITTLTVEPNVLNITFRTFVEVVFENHEK 437

Query: 423 SLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQVFPKSWAAILLTFDNAGM 482
           S+QS+HLDGYSFFAVA EPGRW+PEKR NYNLLDAVSRHT+QV+PKSW+AILLTFDNAGM
Sbjct: 438 SMQSFHLDGYSFFAVASEPGRWTPEKRNNYNLLDAVSRHTVQVYPKSWSAILLTFDNAGM 497

Query: 483 WNLRSELLENRYLGSQLYVSVLSPARSLRDEYNIPDNTLLCGLVKDMPLPKPYTI 538
           WN+RSE  E RYLG QLYVSVLSP +SLRDEYNIP NT LCG+VK +PLP PYTI
Sbjct: 498 WNIRSENWERRYLGQQLYVSVLSPEKSLRDEYNIPLNTNLCGIVKGLPLPTPYTI 551

BLAST of Cucsa.152620.1 vs. TAIR10
Match: AT1G55560.1 (AT1G55560.1 SKU5 similar 14)

HSP 1 Score: 865.9 bits (2236), Expect = 1.3e-251
Identity = 409/534 (76.59%), Postives = 463/534 (86.70%), Query Frame = 1

Query: 3   ATVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNNLVINVFNNLDE 62
           A V   DPYFF TWNVTYGT SPLGVPQ+ ILINGQFPGPN+NSTSNNN+VINVFN+LDE
Sbjct: 17  AIVNAGDPYFFHTWNVTYGTASPLGVPQKVILINGQFPGPNLNSTSNNNVVINVFNHLDE 76

Query: 63  PFLLHWSGIQHRKNSWQDGLLGTNCPILPGTNYTYHFQVKDQIGSFFYYPSTAMHRAAGG 122
           PFLL WSGIQHRKN WQDG+ GT+CPI  G N+TYHFQ KDQIGS+FYYP+T++HR AGG
Sbjct: 77  PFLLTWSGIQHRKNCWQDGVAGTSCPIPAGQNFTYHFQPKDQIGSYFYYPTTSLHRFAGG 136

Query: 123 FGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRSIARPDGVLINGKS 182
           FGGLRVNSRLLIPVPYADPEDDYTVL+GDWYT  HTALK  LDSGR++  P+GVLINGKS
Sbjct: 137 FGGLRVNSRLLIPVPYADPEDDYTVLLGDWYTAGHTALKNFLDSGRTLGLPNGVLINGKS 196

Query: 183 AKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMKLVEMEGSHTVQNDYESLD 242
            K  G +EPLFTMKPGKTYKYR+CNVG KS+LNFR Q H MKLVEMEGSH +QNDY+SLD
Sbjct: 197 GKVGGKNEPLFTMKPGKTYKYRLCNVGFKSTLNFRIQNHKMKLVEMEGSHVIQNDYDSLD 256

Query: 243 VHVGQCFSVLVTANQEPKDYYMVASTRFIKNVLVGKGIVRYIDGKGPASPEIPEAPMGWA 302
           VHVGQCFSVLVTANQ  KDYYMVASTRF+K  L   G++RY      AS E+P+AP+GWA
Sbjct: 257 VHVGQCFSVLVTANQAAKDYYMVASTRFLKKELSTVGVIRYEGSNVQASTELPKAPVGWA 316

Query: 303 WSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSISKVDGKLRYAINGVSHV 362
           WSLNQFR+FRWNLT++AARPNPQGSYHYG INITR+IKLVNS S VDGK+R+  NGVSHV
Sbjct: 317 WSLNQFRSFRWNLTSNAARPNPQGSYHYGKINITRSIKLVNSKSVVDGKVRFGFNGVSHV 376

Query: 363 DPETPLKLAEYFEVADKVFKYDTISDEGLAEGATTVTVAPNVVNATFRNFIEIIFENHEK 422
           D ETPLKLAEYF++++KVFKY+ I DE  A+  T +TV PNV+N TFR F+EIIFENHEK
Sbjct: 377 DTETPLKLAEYFQMSEKVFKYNVIKDEPAAK-ITALTVQPNVLNITFRTFVEIIFENHEK 436

Query: 423 SLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQVFPKSWAAILLTFDNAGM 482
           ++QS+HLDGYSFFAVA EPGRW+PEKR NYNLLDAVSRHT+QV+PKSW+AILLTFDNAGM
Sbjct: 437 TMQSFHLDGYSFFAVASEPGRWTPEKRENYNLLDAVSRHTVQVYPKSWSAILLTFDNAGM 496

Query: 483 WNLRSELLENRYLGSQLYVSVLSPARSLRDEYNIPDNTLLCGLVKDMPLPKPYT 537
           WN+RSE LE +YLG QLYVSVLSP +SLRDEYNIP NT LCG+VK +PLP  Y+
Sbjct: 497 WNIRSENLERKYLGEQLYVSVLSPEKSLRDEYNIPLNTNLCGIVKGLPLPAHYS 549

BLAST of Cucsa.152620.1 vs. TAIR10
Match: AT1G21860.1 (AT1G21860.1 SKU5 similar 7)

HSP 1 Score: 625.5 bits (1612), Expect = 2.9e-179
Identity = 301/517 (58.22%), Postives = 381/517 (73.69%), Query Frame = 1

Query: 8   EDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNNLVINVFNNLDEPFLLH 67
           EDPY FF W+VTYG ISPLGV QQGILING+FPGP+I S +N+NL+INVFN+LDEPFLL 
Sbjct: 25  EDPYRFFEWHVTYGNISPLGVAQQGILINGKFPGPDIISITNDNLIINVFNHLDEPFLLS 84

Query: 68  WSGIQHRKNSWQDGLLGTNCPILPGTNYTYHFQVKDQIGSFFYYPSTAMHRAAGGFGGLR 127
           W+GI++ KNS+QDG+ GT CPI PG NYTY  QVKDQIGSF+Y+PS   H+AAGGFGG+R
Sbjct: 85  WNGIRNWKNSFQDGVYGTMCPIPPGKNYTYALQVKDQIGSFYYFPSLGFHKAAGGFGGIR 144

Query: 128 VNSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRSIARPDGVLINGKSAKGDG 187
           ++SR LIPVP+  P DDYT+L+GDWY  +H  LK  LD+G  +  PDG+LING+S+    
Sbjct: 145 ISSRALIPVPFPTPADDYTLLVGDWYKTNHKDLKAQLDNGGKLPLPDGILINGRSSGA-- 204

Query: 188 TDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMKLVEMEGSHTVQNDYESLDVHVGQ 247
                  ++PGKTY+ R+ NVGL++SLNFR Q HTMKLVE+EG +T+QN + SLDVHVGQ
Sbjct: 205 ----TLNIEPGKTYRLRISNVGLQNSLNFRIQNHTMKLVEVEGRYTIQNLFSSLDVHVGQ 264

Query: 248 CFSVLVTANQEPKDYYMVASTRFIKNVLVGKGIVRYIDGKGPASPEIPEAPMGWAWSLNQ 307
            +SVL+TA+Q  KDYY+V S+RF   +L   G++ Y +   P S  IP+ P+  +WS NQ
Sbjct: 265 SYSVLITADQPAKDYYVVVSSRFTSKILTTTGVLHYSNSVAPVSGPIPDGPIKLSWSFNQ 324

Query: 308 FRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSISKVDGKLRYAINGVSHVDPETP 367
            R  R NLTAS  RPNPQGSY YG INITRTI+L N++  ++GK RYA+N  S    +TP
Sbjct: 325 ARAIRTNLTASGPRPNPQGSYRYGVINITRTIRLANNLGHIEGKQRYAVNSASFYPADTP 384

Query: 368 LKLAEYFEVADKVFKYDTISDEGLAEGATTVTVAPNVVNATFRNFIEIIFENHEKSLQSW 427
           LKL +YF++ D V+K  +ISD+    GA   T   +V+ A FR F+E+IFEN E  +QSW
Sbjct: 385 LKLVDYFKI-DGVYKPGSISDQP-TNGAIFPTT--SVMQADFRAFVEVIFENSEDIVQSW 444

Query: 428 HLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQVFPKSWAAILLTFDNAGMWNLRS 487
           HLDGYSF+ V +E G+WSP  R  YNL DA+ R TIQV+P+SW AI +  DN GMWN+RS
Sbjct: 445 HLDGYSFYVVGMELGKWSPASRKVYNLNDAILRCTIQVYPRSWTAIYIALDNVGMWNMRS 504

Query: 488 ELLENRYLGSQLYVSVLSPARSLRDEYNIPDNTLLCG 525
           E+ E +YLG Q Y+ V + + SLRDEY IP N LLCG
Sbjct: 505 EIWERQYLGQQFYMRVYTTSTSLRDEYLIPKNALLCG 531

BLAST of Cucsa.152620.1 vs. NCBI nr
Match: gi|778692720|ref|XP_011653513.1| (PREDICTED: L-ascorbate oxidase homolog [Cucumis sativus])

HSP 1 Score: 1079.7 bits (2791), Expect = 0.0e+00
Identity = 520/538 (96.65%), Postives = 529/538 (98.33%), Query Frame = 1

Query: 1   MTATVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNNLVINVFNNL 60
           M ATVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPN+NST+NNNLVINVFNNL
Sbjct: 17  MMATVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNVNSTTNNNLVINVFNNL 76

Query: 61  DEPFLLHWSGIQHRKNSWQDGLLGTNCPILPGTNYTYHFQVKDQIGSFFYYPSTAMHRAA 120
           DEPFLLHWSGIQHRKNSWQDGLLGTNCPI PGTN+TYHFQVKDQIGSFFYYPSTAMHRAA
Sbjct: 77  DEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQIGSFFYYPSTAMHRAA 136

Query: 121 GGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQL-LDSGRSIARPDGVLIN 180
           GGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHT LKQ+ LDSGRSIARPDGVLIN
Sbjct: 137 GGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQIFLDSGRSIARPDGVLIN 196

Query: 181 GKSAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMKLVEMEGSHTVQNDYE 240
           GK+AKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMKLVEMEGSHTVQNDY+
Sbjct: 197 GKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMKLVEMEGSHTVQNDYQ 256

Query: 241 SLDVHVGQCFSVLVTANQEPKDYYMVASTRFIKNVLVGKGIVRYIDGKGPASPEIPEAPM 300
           SLDVHVGQCFSVLVTANQEPKDYYMVASTRFIKNVLVGKGIVRY DGKG ASPEIPEAP+
Sbjct: 257 SLDVHVGQCFSVLVTANQEPKDYYMVASTRFIKNVLVGKGIVRYTDGKGHASPEIPEAPV 316

Query: 301 GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSISKVDGKLRYAINGV 360
           GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNS+SKVDGKLRYAINGV
Sbjct: 317 GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSVSKVDGKLRYAINGV 376

Query: 361 SHVDPETPLKLAEYFEVADKVFKYDTISDEGLAEGATTVTVAPNVVNATFRNFIEIIFEN 420
           SHVDPETPLKLAEYFE+ADKVFKYDTISDEGLAEG TTVTVAPNVVN TFRNFIEIIFEN
Sbjct: 377 SHVDPETPLKLAEYFEIADKVFKYDTISDEGLAEGVTTVTVAPNVVNTTFRNFIEIIFEN 436

Query: 421 HEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQVFPKSWAAILLTFDN 480
           HEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQVFPKSWAAILLTFDN
Sbjct: 437 HEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQVFPKSWAAILLTFDN 496

Query: 481 AGMWNLRSELLENRYLGSQLYVSVLSPARSLRDEYNIPDNTLLCGLVKDMPLPKPYTI 538
           AGMWNLRSELLENRYLGSQLYVSV SPARSLRDEYNIPDNTLLCGLVKDMPLPKPYTI
Sbjct: 497 AGMWNLRSELLENRYLGSQLYVSVPSPARSLRDEYNIPDNTLLCGLVKDMPLPKPYTI 554

BLAST of Cucsa.152620.1 vs. NCBI nr
Match: gi|659097981|ref|XP_008449913.1| (PREDICTED: L-ascorbate oxidase homolog isoform X2 [Cucumis melo])

HSP 1 Score: 1047.7 bits (2708), Expect = 6.8e-303
Identity = 496/534 (92.88%), Postives = 519/534 (97.19%), Query Frame = 1

Query: 5   VRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNNLVINVFNNLDEPF 64
           VRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNN+V+NVFNNL+EPF
Sbjct: 21  VRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNNIVVNVFNNLNEPF 80

Query: 65  LLHWSGIQHRKNSWQDGLLGTNCPILPGTNYTYHFQVKDQIGSFFYYPSTAMHRAAGGFG 124
           LLHWSGIQHRKNSWQDGLLGTNCPI PG N+TYHFQVKDQIGSFFYYPS AMHRAAGGFG
Sbjct: 81  LLHWSGIQHRKNSWQDGLLGTNCPIPPGANFTYHFQVKDQIGSFFYYPSIAMHRAAGGFG 140

Query: 125 GLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSG-RSIARPDGVLINGKSA 184
           GLR+NSRLLIPVPYADPEDDYTVLIGDWYTKSHT LKQ+LD+G RSIARP+GVLINGK+A
Sbjct: 141 GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQILDNGHRSIARPEGVLINGKTA 200

Query: 185 KGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMKLVEMEGSHTVQNDYESLDV 244
           KGDGTDEPLFTMKPGKTYKYRVCNVGLK+SLNFRFQGHTMKLVEMEGSHTVQNDY+SLDV
Sbjct: 201 KGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTMKLVEMEGSHTVQNDYQSLDV 260

Query: 245 HVGQCFSVLVTANQEPKDYYMVASTRFIKNVLVGKGIVRYIDGKGPASPEIPEAPMGWAW 304
           HVGQCFSVLVTANQEPKDYYMVASTRFIKNV++GKGI+RY +GKGPASPEIP+APMGWAW
Sbjct: 261 HVGQCFSVLVTANQEPKDYYMVASTRFIKNVIIGKGIIRYTNGKGPASPEIPKAPMGWAW 320

Query: 305 SLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSISKVDGKLRYAINGVSHVD 364
           SLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKL NS +KVDGKLRYAINGVSH+D
Sbjct: 321 SLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLFNSATKVDGKLRYAINGVSHID 380

Query: 365 PETPLKLAEYFEVADKVFKYDTISDEGLAEGATTVTVAPNVVNATFRNFIEIIFENHEKS 424
           PETPLKLAEYF +ADKVFKYD ISDEG  EGAT VT+APNVVNATFRNFIEIIFENHEKS
Sbjct: 381 PETPLKLAEYFGIADKVFKYDIISDEGPTEGATAVTIAPNVVNATFRNFIEIIFENHEKS 440

Query: 425 LQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQVFPKSWAAILLTFDNAGMW 484
           LQSWHLDGYSFFAVAIEPGRWSPEKR+NYNLLDAVSRHTIQVFP SWAAILLTFDNAGMW
Sbjct: 441 LQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTIQVFPNSWAAILLTFDNAGMW 500

Query: 485 NLRSELLENRYLGSQLYVSVLSPARSLRDEYNIPDNTLLCGLVKDMPLPKPYTI 538
           NLRSEL ENRYLGSQLY+SVLSPARSLRDEYNIPDNTLLCGLVKDMP+PKPYTI
Sbjct: 501 NLRSELTENRYLGSQLYISVLSPARSLRDEYNIPDNTLLCGLVKDMPMPKPYTI 554

BLAST of Cucsa.152620.1 vs. NCBI nr
Match: gi|778692705|ref|XP_011653509.1| (PREDICTED: L-ascorbate oxidase homolog [Cucumis sativus])

HSP 1 Score: 1045.8 bits (2703), Expect = 2.6e-302
Identity = 497/534 (93.07%), Postives = 519/534 (97.19%), Query Frame = 1

Query: 5   VRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNNLVINVFNNLDEPF 64
           VRGEDPYFFFTWN+TYGTISPLGVPQQGILINGQFPGPNINSTSNNN+VINVFNNL+EPF
Sbjct: 20  VRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNINSTSNNNIVINVFNNLNEPF 79

Query: 65  LLHWSGIQHRKNSWQDGLLGTNCPILPGTNYTYHFQVKDQIGSFFYYPSTAMHRAAGGFG 124
           LLHWSGIQHRKNSWQDGLLGTNCPILPGTN+TYHFQVKDQIGSFFYYP  AMHRAAGGFG
Sbjct: 80  LLHWSGIQHRKNSWQDGLLGTNCPILPGTNFTYHFQVKDQIGSFFYYPPIAMHRAAGGFG 139

Query: 125 GLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSG-RSIARPDGVLINGKSA 184
           GLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHT LKQ+LD+G RSIARP+GVLINGK+A
Sbjct: 140 GLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQILDNGHRSIARPEGVLINGKTA 199

Query: 185 KGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMKLVEMEGSHTVQNDYESLDV 244
           KGDGTDEPLFTMKP KTYKYRVCNVGLK+SLNFRFQGHTMKLVEMEGSHTVQNDY+SLDV
Sbjct: 200 KGDGTDEPLFTMKPEKTYKYRVCNVGLKTSLNFRFQGHTMKLVEMEGSHTVQNDYQSLDV 259

Query: 245 HVGQCFSVLVTANQEPKDYYMVASTRFIKNVLVGKGIVRYIDGKGPASPEIPEAPMGWAW 304
           HVGQCFSVLVTANQEPKDYYMVASTRFIKNVL+GKGI+RY +GKGPASPEIP+APMGWAW
Sbjct: 260 HVGQCFSVLVTANQEPKDYYMVASTRFIKNVLIGKGIIRYTNGKGPASPEIPKAPMGWAW 319

Query: 305 SLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSISKVDGKLRYAINGVSHVD 364
           SLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKL NS +KVDGKLRYAINGVSHVD
Sbjct: 320 SLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLFNSATKVDGKLRYAINGVSHVD 379

Query: 365 PETPLKLAEYFEVADKVFKYDTISDEGLAEGATTVTVAPNVVNATFRNFIEIIFENHEKS 424
           PETPLKLAEYFE+A+KVFKYD ISDEG  EGAT VT+APNVVNATFRNFIEIIFENHEKS
Sbjct: 380 PETPLKLAEYFEIAEKVFKYDMISDEGPTEGATAVTIAPNVVNATFRNFIEIIFENHEKS 439

Query: 425 LQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQVFPKSWAAILLTFDNAGMW 484
           LQSWHLDGYSFFAVAIEPGRWSPEKR+NYNLLDAVSRHTIQVFP SWAAILLTFDNAGMW
Sbjct: 440 LQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTIQVFPNSWAAILLTFDNAGMW 499

Query: 485 NLRSELLENRYLGSQLYVSVLSPARSLRDEYNIPDNTLLCGLVKDMPLPKPYTI 538
           NLRSEL+ENRYLG QLY+SVLSPARSLRDEYNIPD+TLLCGLVK MPLPKPYTI
Sbjct: 500 NLRSELIENRYLGQQLYISVLSPARSLRDEYNIPDHTLLCGLVKGMPLPKPYTI 553

BLAST of Cucsa.152620.1 vs. NCBI nr
Match: gi|659097979|ref|XP_008449912.1| (PREDICTED: L-ascorbate oxidase homolog isoform X1 [Cucumis melo])

HSP 1 Score: 1041.6 bits (2692), Expect = 4.9e-301
Identity = 494/534 (92.51%), Postives = 517/534 (96.82%), Query Frame = 1

Query: 5   VRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNNLVINVFNNLDEPF 64
           VRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNN+V+NVFNNL+EPF
Sbjct: 21  VRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNNIVVNVFNNLNEPF 80

Query: 65  LLHWSGIQHRKNSWQDGLLGTNCPILPGTNYTYHFQVKDQIGSFFYYPSTAMHRAAGGFG 124
           LLHWSGIQHRKNSWQDGLLGTNCPI PG N+TYHFQVKDQIGSFFYYPS AMHRAAGGFG
Sbjct: 81  LLHWSGIQHRKNSWQDGLLGTNCPIPPGANFTYHFQVKDQIGSFFYYPSIAMHRAAGGFG 140

Query: 125 GLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSG-RSIARPDGVLINGKSA 184
           GLR+NSRLLIPVPYADPEDDYTVLIGDWYTKSHT LKQ+LD+G RSIARP+GVLINGK+A
Sbjct: 141 GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQILDNGHRSIARPEGVLINGKTA 200

Query: 185 KGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMKLVEMEGSHTVQNDYESLDV 244
           KGDGTDEPLFTMKPGKTYKYRVCNVGLK+SLNFRFQGHTMKLVEMEGSHTVQNDY+SLDV
Sbjct: 201 KGDGTDEPLFTMKPGKTYKYRVCNVGLKTSLNFRFQGHTMKLVEMEGSHTVQNDYQSLDV 260

Query: 245 HVGQCFSVLVTANQEPKDYYMVASTRFIKNVLVGKGIVRYIDGKGPASPEIPEAPMGWAW 304
           HVGQCFSVLVTANQEPKDYYMVASTRFIKNV++GKGI+RY +GKGPASPEIP+APMGWAW
Sbjct: 261 HVGQCFSVLVTANQEPKDYYMVASTRFIKNVIIGKGIIRYTNGKGPASPEIPKAPMGWAW 320

Query: 305 SLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSISKVDGKLRYAINGVSHVD 364
           SLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKL NS +KVDGKLRYAINGVSH+D
Sbjct: 321 SLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLFNSATKVDGKLRYAINGVSHID 380

Query: 365 PETPLKLAEYFEVADKVFKYDTISDEGLAEGATTVTVAPNVVNATFRNFIEIIFENHEKS 424
           PETPLKLAEYF +ADKVFKYD ISDEG  EGAT VT+APNVVNATFRNFIEIIFENHEKS
Sbjct: 381 PETPLKLAEYFGIADKVFKYDIISDEGPTEGATAVTIAPNVVNATFRNFIEIIFENHEKS 440

Query: 425 LQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQVFPKSWAAILLTFDNAGMW 484
           LQSWHLDGYSFFAVAIEPGRWSPEKR+NYNLLDAVSRHTIQVFP SWAAILLTFDNAGMW
Sbjct: 441 LQSWHLDGYSFFAVAIEPGRWSPEKRSNYNLLDAVSRHTIQVFPNSWAAILLTFDNAGMW 500

Query: 485 NLRSELLENRYLGSQLYVSVLSPARSLRDEYNIPDNTLLCGLVKDMPLPKPYTI 538
           NLRSEL ENRYLG QLY+SVLSPARSLRDEYNIPD+TLLCGLVK MPLPKPYTI
Sbjct: 501 NLRSELTENRYLGQQLYMSVLSPARSLRDEYNIPDHTLLCGLVKGMPLPKPYTI 554

BLAST of Cucsa.152620.1 vs. NCBI nr
Match: gi|449439217|ref|XP_004137383.1| (PREDICTED: L-ascorbate oxidase homolog [Cucumis sativus])

HSP 1 Score: 1021.5 bits (2640), Expect = 5.2e-295
Identity = 484/534 (90.64%), Postives = 508/534 (95.13%), Query Frame = 1

Query: 4   TVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNNLVINVFNNLDEP 63
           TVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINST+NNNLVINVFNNLDEP
Sbjct: 20  TVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTTNNNLVINVFNNLDEP 79

Query: 64  FLLHWSGIQHRKNSWQDGLLGTNCPILPGTNYTYHFQVKDQIGSFFYYPSTAMHRAAGGF 123
           FLLHWSGIQHRKNSWQDGLLGTNCPI PGTN+TYHFQVKDQIGSFFYYPSTAMH+A G F
Sbjct: 80  FLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQIGSFFYYPSTAMHKAVGAF 139

Query: 124 GGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRSIARPDGVLINGKSA 183
           GGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSH+ LK+ LDSGRSIARPDGVLING+ A
Sbjct: 140 GGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDSGRSIARPDGVLINGRIA 199

Query: 184 KGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMKLVEMEGSHTVQNDYESLDV 243
           KGDGTDEPLFTMKP KTYKYR+CNVGLK+SLNFR QGHTMKLVEMEGSHTVQN Y+SLDV
Sbjct: 200 KGDGTDEPLFTMKPEKTYKYRICNVGLKTSLNFRIQGHTMKLVEMEGSHTVQNVYQSLDV 259

Query: 244 HVGQCFSVLVTANQEPKDYYMVASTRFIKNVLVGKGIVRYIDGKGPASPEIPEAPMGWAW 303
           HVGQCFSVLVTA+++PKDYYMVASTRF KN+LVGKGI+RY +GK PASPEIPEAP GWAW
Sbjct: 260 HVGQCFSVLVTADKDPKDYYMVASTRFTKNILVGKGIIRYTNGKAPASPEIPEAPRGWAW 319

Query: 304 SLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSISKVDGKLRYAINGVSHVD 363
           SLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK+VNS + VDGKLRYAINGVSHVD
Sbjct: 320 SLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNSATNVDGKLRYAINGVSHVD 379

Query: 364 PETPLKLAEYFEVADKVFKYDTISDEGLAEGATTVTVAPNVVNATFRNFIEIIFENHEKS 423
           PETPLKLAEYFEVADKVFKYDTISDEGL++ A  VTVAPNV+NATFRNF+EI+FEN EKS
Sbjct: 380 PETPLKLAEYFEVADKVFKYDTISDEGLSKTANIVTVAPNVLNATFRNFVEIVFENQEKS 439

Query: 424 LQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQVFPKSWAAILLTFDNAGMW 483
           LQSWHLDGYSFFAVA+EPGRWSPEKR NYNLLDAVSRHTIQVFPKSWAAILLT DNAGMW
Sbjct: 440 LQSWHLDGYSFFAVAVEPGRWSPEKRKNYNLLDAVSRHTIQVFPKSWAAILLTLDNAGMW 499

Query: 484 NLRSELLENRYLGSQLYVSVLSPARSLRDEYNIPDNTLLCGLVKDMPLPKPYTI 538
           NLRSEL ENRYLG Q+Y SV SPARSLRDEYNIPDNTLLCGLVKDMPLP PYTI
Sbjct: 500 NLRSELTENRYLGQQMYFSVQSPARSLRDEYNIPDNTLLCGLVKDMPLPPPYTI 553