Cucsa.147540.2 (mRNA) Cucumber (Gy14) v1

BLAST of Cucsa.147540.2 vs. Swiss-Prot
Match: CAPPC_FLATR (Phosphoenolpyruvate carboxylase 2 OS=Flaveria trinervia GN=PPCC PE=1 SV=1)

HSP 1 Score: 1321.6 bits (3419), Expect = 0.0e+00
Identity = 649/740 (87.70%), Postives = 705/740 (95.27%), Query Frame = 1

Query: 3   AVKNLEKMASIDAQLRLLAPSKVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQECY 62
           A +NLEK+ASIDAQLRLL P KVSEDDKL+EYDALLLD+FLDILQDLHGE L+ETVQECY
Sbjct: 2   ANRNLEKLASIDAQLRLLVPGKVSEDDKLIEYDALLLDKFLDILQDLHGEGLKETVQECY 61

Query: 63  EFAAEYERKRDPEKLEELGNALTSLDPGDSIVVAKSFSHMLSLANLAEEVQIAYRRRIKL 122
           E +AEYE K DP+KLEELGN LTSLDPGDSIV+AK+FSHML+LANLAEEVQIAYRRRIKL
Sbjct: 62  ELSAEYEGKHDPKKLEELGNVLTSLDPGDSIVIAKAFSHMLNLANLAEEVQIAYRRRIKL 121

Query: 123 KKGGFADEANATTESDIEETFKRLL-QLNKSPQEVFDALKNQTVDLVFTAHPTQSVRRSL 182
           KKG FADEA+ATTESDIEETFK+L+ +L KSP+EVFDALKNQTVDLVFTAHPTQSVRRSL
Sbjct: 122 KKGDFADEASATTESDIEETFKKLVHKLKKSPEEVFDALKNQTVDLVFTAHPTQSVRRSL 181

Query: 183 LQKHGRIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMS 242
           LQKHGRIR+CL QLYAKDITPDDKQELDEAL REIQAAFRTDEIRRTPPTPQDEMRAGMS
Sbjct: 182 LQKHGRIRDCLAQLYAKDITPDDKQELDEALHREIQAAFRTDEIRRTPPTPQDEMRAGMS 241

Query: 243 YFHETIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRD 302
           YFHETIWKGVPKFLRRVDTALKNIGI+ERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRD
Sbjct: 242 YFHETIWKGVPKFLRRVDTALKNIGINERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRD 301

Query: 303 VCLLARMMAANLYFSQIEDLMFELSMWRCSSELQTRAEELHRS-SRKDAKHYIEFWKQIP 362
           VCLLARMMAAN+YFSQIEDLMFE+SMWRCS EL+ RAEELHRS S++D KHYIEFWKQ+P
Sbjct: 302 VCLLARMMAANMYFSQIEDLMFEMSMWRCSDELRVRAEELHRSSSKRDVKHYIEFWKQVP 361

Query: 363 PNEPYRVILGDVRDKLYNTRERARHLLSSGMSEIPEDTTFTDVEQFLEPLELCYRSLCAC 422
           P EPYRVILGDVRDKLYNTRERARHLL+  +S+IPE++ +T+VEQFLEPLELCYRSLCAC
Sbjct: 362 PTEPYRVILGDVRDKLYNTRERARHLLAHDVSDIPEESVYTNVEQFLEPLELCYRSLCAC 421

Query: 423 GDRPIADGSLLDFLRQVSTFGLSLVRLDIRQESERHADVIDTITNYLGIGSYKEWSEEQK 482
           GDR IADGSLLDFLRQVSTFGLSLVRLDIRQES+RH DV+D IT +L IGSY+EWSEE++
Sbjct: 422 GDRVIADGSLLDFLRQVSTFGLSLVRLDIRQESDRHTDVLDAITQHLEIGSYREWSEEKR 481

Query: 483 QEWLLSELSGKRPLFGPDLPKTEETSDVLDTFSVIAELPSDNFGAYIISMATAPSDVLAV 542
           QEWLLSELSGKRPLFGPDLPKTEE +DVLDTF V+AELP+D FGAYIISMAT+PSDVLAV
Sbjct: 482 QEWLLSELSGKRPLFGPDLPKTEEIADVLDTFHVLAELPADCFGAYIISMATSPSDVLAV 541

Query: 543 ELLQRECHVQKPLRVVPLFEKLADLEAAPSAMARLFSIDWYRNRIDGKQEVMIGYSDSGK 602
           ELLQRECHV++PLRVVPLFEKLADLEAAP+A+ARLFSI+WY+NRIDGKQEVMIGYSDSGK
Sbjct: 542 ELLQRECHVKQPLRVVPLFEKLADLEAAPAAVARLFSIEWYKNRIDGKQEVMIGYSDSGK 601

Query: 603 DAGRLSAAWQLYKAQEELIQVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHG 662
           DAGRLSAAWQLYKAQEELI VAK++GVKLTMFHGRGGTVGRGGGPTHLAILSQPP+TIHG
Sbjct: 602 DAGRLSAAWQLYKAQEELINVAKKFGVKLTMFHGRGGTVGRGGGPTHLAILSQPPETIHG 661

Query: 663 SLRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMNPPVAPKPEWRALLDAMAVVATE 722
           SLRVTVQGEVIEQSFGEEHLCFRTLQRF AATLEHGMNPP++P+PEWRAL+D MAV ATE
Sbjct: 662 SLRVTVQGEVIEQSFGEEHLCFRTLQRFCAATLEHGMNPPISPRPEWRALMDEMAVYATE 721

Query: 723 KYRSIVFQEPRFVEYFRLVS 741
           +YR IVF+EPRFVEYFRL +
Sbjct: 722 QYREIVFKEPRFVEYFRLAT 741

BLAST of Cucsa.147540.2 vs. Swiss-Prot
Match: CAPP2_ARATH (Phosphoenolpyruvate carboxylase 2 OS=Arabidopsis thaliana GN=PPC2 PE=1 SV=2)

HSP 1 Score: 1316.2 bits (3405), Expect = 0.0e+00
Identity = 649/739 (87.82%), Postives = 700/739 (94.72%), Query Frame = 1

Query: 3   AVKNLEKMASIDAQLRLLAPSKVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQECY 62
           A +NLEKMASIDAQLRLLAP KVSEDDKL+EYDALLLDRFLDILQDLHGED+RE VQECY
Sbjct: 2   AARNLEKMASIDAQLRLLAPGKVSEDDKLIEYDALLLDRFLDILQDLHGEDVREFVQECY 61

Query: 63  EFAAEYERKRDPEKLEELGNALTSLDPGDSIVVAKSFSHMLSLANLAEEVQIAYRRRIK- 122
           E AA+Y+  R+ EKLEELGN LTSLDPGDSIVV KSFS+MLSLANLAEEVQIAYRRRIK 
Sbjct: 62  EVAADYDGNRNTEKLEELGNMLTSLDPGDSIVVTKSFSNMLSLANLAEEVQIAYRRRIKK 121

Query: 123 LKKGGFADEANATTESDIEETFKRLLQLNKSPQEVFDALKNQTVDLVFTAHPTQSVRRSL 182
           LKKG FADEA+ATTESDIEET KRLLQLNK+P+EVFDALKNQTVDLV TAHPTQSVRRSL
Sbjct: 122 LKKGDFADEASATTESDIEETLKRLLQLNKTPEEVFDALKNQTVDLVLTAHPTQSVRRSL 181

Query: 183 LQKHGRIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMS 242
           LQK GRIR+CLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMS
Sbjct: 182 LQKFGRIRDCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMS 241

Query: 243 YFHETIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRD 302
           YFHETIWKGVPKFLRRVDTALKNIGI+ERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRD
Sbjct: 242 YFHETIWKGVPKFLRRVDTALKNIGINERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRD 301

Query: 303 VCLLARMMAANLYFSQIEDLMFELSMWRCSSELQTRAEELHRSSRKDAKHYIEFWKQIPP 362
           VCLLARMMAANLYFSQIEDLMFE+SMWRC+ EL+ RAE   R +++DAKHYIEFWKQIP 
Sbjct: 302 VCLLARMMAANLYFSQIEDLMFEMSMWRCNEELRVRAER-QRCAKRDAKHYIEFWKQIPA 361

Query: 363 NEPYRVILGDVRDKLYNTRERARHLLSSGMSEIPEDTTFTDVEQFLEPLELCYRSLCACG 422
           NEPYR ILGDVRDKLYNTRERAR LLSSG+S++PED  FT V+QFLEPLELCYRSLC CG
Sbjct: 362 NEPYRAILGDVRDKLYNTRERARQLLSSGVSDVPEDAVFTSVDQFLEPLELCYRSLCDCG 421

Query: 423 DRPIADGSLLDFLRQVSTFGLSLVRLDIRQESERHADVIDTITNYLGIGSYKEWSEEQKQ 482
           DRPIADGSLLDFLRQVSTFGL+LV+LDIRQESERH+DV+D IT +LGIGSYKEWSE+++Q
Sbjct: 422 DRPIADGSLLDFLRQVSTFGLALVKLDIRQESERHSDVLDAITTHLGIGSYKEWSEDKRQ 481

Query: 483 EWLLSELSGKRPLFGPDLPKTEETSDVLDTFSVIAELPSDNFGAYIISMATAPSDVLAVE 542
           EWLLSELSGKRPLFGPDLPKTEE +DVLDTF VI+ELPSD+FGAYIISMATAPSDVLAVE
Sbjct: 482 EWLLSELSGKRPLFGPDLPKTEEVADVLDTFKVISELPSDSFGAYIISMATAPSDVLAVE 541

Query: 543 LLQRECHVQKPLRVVPLFEKLADLEAAPSAMARLFSIDWYRNRIDGKQEVMIGYSDSGKD 602
           LLQREC +  PLRVVPLFEKLADLE+AP+A+ARLFSI+WYRNRI+GKQEVMIGYSDSGKD
Sbjct: 542 LLQRECGITDPLRVVPLFEKLADLESAPAAVARLFSIEWYRNRINGKQEVMIGYSDSGKD 601

Query: 603 AGRLSAAWQLYKAQEELIQVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHGS 662
           AGRLSAAWQLYK QEEL++VAK+YGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHG 
Sbjct: 602 AGRLSAAWQLYKTQEELVKVAKEYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHGQ 661

Query: 663 LRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMNPPVAPKPEWRALLDAMAVVATEK 722
           LRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGM+PPV+PKPEWR L+D MA++ATE+
Sbjct: 662 LRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMHPPVSPKPEWRVLMDEMAIIATEE 721

Query: 723 YRSIVFQEPRFVEYFRLVS 741
           YRS+VF+EPRFVEYFRL +
Sbjct: 722 YRSVVFKEPRFVEYFRLAT 739

BLAST of Cucsa.147540.2 vs. Swiss-Prot
Match: CAPP1_FLAPR (Phosphoenolpyruvate carboxylase OS=Flaveria pringlei GN=PPCA1 PE=1 SV=1)

HSP 1 Score: 1315.1 bits (3402), Expect = 0.0e+00
Identity = 644/740 (87.03%), Postives = 703/740 (95.00%), Query Frame = 1

Query: 3   AVKNLEKMASIDAQLRLLAPSKVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQECY 62
           A +NLEK+ASIDAQLRLL P KVSEDDKL+EYDALLLD+FLDILQDLHGEDL+E VQECY
Sbjct: 2   ANRNLEKLASIDAQLRLLVPGKVSEDDKLIEYDALLLDKFLDILQDLHGEDLKEAVQECY 61

Query: 63  EFAAEYERKRDPEKLEELGNALTSLDPGDSIVVAKSFSHMLSLANLAEEVQIAYRRRIKL 122
           E +AEYE K DP+KLEELG+ LTSLDPGDSIV+AK+FSHML+LANLAEEVQIAYRRRIKL
Sbjct: 62  ELSAEYEGKHDPKKLEELGSVLTSLDPGDSIVIAKAFSHMLNLANLAEEVQIAYRRRIKL 121

Query: 123 KKGGFADEANATTESDIEETFKRL-LQLNKSPQEVFDALKNQTVDLVFTAHPTQSVRRSL 182
           K+G FADEANATTESDIEETFK+L L+LNKSP+EVFDALKNQTVDLV TAHPTQSVRRSL
Sbjct: 122 KRGDFADEANATTESDIEETFKKLVLKLNKSPEEVFDALKNQTVDLVLTAHPTQSVRRSL 181

Query: 183 LQKHGRIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMS 242
           LQKHGRIRNCL QLYAKDITPDDKQELDEAL REIQAAFRTDEIRRTPPTPQDEMRAGMS
Sbjct: 182 LQKHGRIRNCLAQLYAKDITPDDKQELDEALHREIQAAFRTDEIRRTPPTPQDEMRAGMS 241

Query: 243 YFHETIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDG-NPRVTPEVTR 302
           YFHETIWKGVPKFLRRVDTALKNIGI+ERVPYNAPLIQFSSWMGGDRDG +PRVTPEVTR
Sbjct: 242 YFHETIWKGVPKFLRRVDTALKNIGINERVPYNAPLIQFSSWMGGDRDGKHPRVTPEVTR 301

Query: 303 DVCLLARMMAANLYFSQIEDLMFELSMWRCSSELQTRAEELHRSSRKDAKHYIEFWKQIP 362
           DVCLLARMMA+N+YFSQIEDLMFE+SMWRC+SEL+ RAEEL+R++R+D KHYIEFWKQ+P
Sbjct: 302 DVCLLARMMASNMYFSQIEDLMFEMSMWRCNSELRVRAEELYRTARRDVKHYIEFWKQVP 361

Query: 363 PNEPYRVILGDVRDKLYNTRERARHLLSSGMSEIPEDTTFTDVEQFLEPLELCYRSLCAC 422
           P EPYRVILGDVRDKLYNTRER+RHLL+ G+S+IPE+  +T+VEQFLEPLELCYRSLC C
Sbjct: 362 PTEPYRVILGDVRDKLYNTRERSRHLLAHGISDIPEEAVYTNVEQFLEPLELCYRSLCDC 421

Query: 423 GDRPIADGSLLDFLRQVSTFGLSLVRLDIRQESERHADVIDTITNYLGIGSYKEWSEEQK 482
           GDR IADGSLLDFLRQVSTFGLSLV+LDIRQES+RH DV+D IT +L IGSY+EWSEE++
Sbjct: 422 GDRVIADGSLLDFLRQVSTFGLSLVKLDIRQESDRHTDVLDAITQHLEIGSYREWSEEKR 481

Query: 483 QEWLLSELSGKRPLFGPDLPKTEETSDVLDTFSVIAELPSDNFGAYIISMATAPSDVLAV 542
           QEWLL+ELSGKRPLFG DLPKTEE  DVLDTF+V+AELPSD FGAYIISMAT+PSDVLAV
Sbjct: 482 QEWLLAELSGKRPLFGSDLPKTEEVKDVLDTFNVLAELPSDCFGAYIISMATSPSDVLAV 541

Query: 543 ELLQRECHVQKPLRVVPLFEKLADLEAAPSAMARLFSIDWYRNRIDGKQEVMIGYSDSGK 602
           ELLQRECHV+ PLRVVPLFEKLADLEAAP+AMARLFSIDWYRNRIDGKQEVMIGYSDSGK
Sbjct: 542 ELLQRECHVKHPLRVVPLFEKLADLEAAPAAMARLFSIDWYRNRIDGKQEVMIGYSDSGK 601

Query: 603 DAGRLSAAWQLYKAQEELIQVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHG 662
           DAGR SAAWQLYKAQEE+I+VAK++GVKL +FHGRGGTVGRGGGPTHLAILSQPPDTIHG
Sbjct: 602 DAGRFSAAWQLYKAQEEIIKVAKEFGVKLVIFHGRGGTVGRGGGPTHLAILSQPPDTIHG 661

Query: 663 SLRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMNPPVAPKPEWRALLDAMAVVATE 722
           SLRVTVQGEVIEQSFGEEHLCFRTLQRF AATLEHGMNPP++P+PEWR L+D MAVVATE
Sbjct: 662 SLRVTVQGEVIEQSFGEEHLCFRTLQRFCAATLEHGMNPPISPRPEWRELMDQMAVVATE 721

Query: 723 KYRSIVFQEPRFVEYFRLVS 741
           +YRSIVF+EPRFVEYFRL +
Sbjct: 722 EYRSIVFKEPRFVEYFRLAT 741

BLAST of Cucsa.147540.2 vs. Swiss-Prot
Match: CAPP1_SACHY (Phosphoenolpyruvate carboxylase, housekeeping isozyme OS=Saccharum hybrid PE=3 SV=1)

HSP 1 Score: 1312.4 bits (3395), Expect = 0.0e+00
Identity = 641/736 (87.09%), Postives = 697/736 (94.70%), Query Frame = 1

Query: 7   LEKMASIDAQLRLLAPSKVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQECYEFAA 66
           ++K  SIDAQLRLLAP K+S+DDKLVEYDALLLDRFLDILQDLHGED+RETVQECYE AA
Sbjct: 6   VDKATSIDAQLRLLAPQKLSDDDKLVEYDALLLDRFLDILQDLHGEDIRETVQECYELAA 65

Query: 67  EYERKRDPEKLEELGNALTSLDPGDSIVVAKSFSHMLSLANLAEEVQIAYRRRIKLKKGG 126
           EYE K DP+ L+E+GN LTSLDPGDSIV+ KSFSHML LANLAEEVQIAYRRRIKLKKG 
Sbjct: 66  EYENKLDPKMLDEIGNVLTSLDPGDSIVITKSFSHMLILANLAEEVQIAYRRRIKLKKGD 125

Query: 127 FADEANATTESDIEETFKRLL-QLNKSPQEVFDALKNQTVDLVFTAHPTQSVRRSLLQKH 186
           F DE +ATTESDIEET KRL+ QL KSP EVFDALKNQTVDLV TAHPTQSVRRSLLQKH
Sbjct: 126 FVDENSATTESDIEETLKRLMHQLKKSPLEVFDALKNQTVDLVLTAHPTQSVRRSLLQKH 185

Query: 187 GRIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMSYFHE 246
           GRIRNCLTQLYAKDITPD+KQELDEALQREIQAAFRTDEIRR PPTPQDEMRAGMSYFHE
Sbjct: 186 GRIRNCLTQLYAKDITPDEKQELDEALQREIQAAFRTDEIRRAPPTPQDEMRAGMSYFHE 245

Query: 247 TIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRDVCLL 306
           TIWKGVPKFLRRVDTALKNIGI+ER+PYNAP+IQFSSWMGGDRDGNPRVTPE+TRDVCLL
Sbjct: 246 TIWKGVPKFLRRVDTALKNIGINERLPYNAPIIQFSSWMGGDRDGNPRVTPEITRDVCLL 305

Query: 307 ARMMAANLYFSQIEDLMFELSMWRCSSELQTRAEELHRSSRKDA-KHYIEFWKQIPPNEP 366
           ARMMAANLY +QIEDLMFELSMWRCS EL+ + +ELHRSS+KD  KHYIEFWKQ+PP+EP
Sbjct: 306 ARMMAANLYNAQIEDLMFELSMWRCSDELRVKVDELHRSSKKDTTKHYIEFWKQVPPSEP 365

Query: 367 YRVILGDVRDKLYNTRERARHLLSSGMSEIPEDTTFTDVEQFLEPLELCYRSLCACGDRP 426
           YRVIL DVRDKLYNTRERARHLL+SG SEIPE+ TFTDVEQFLEPLELCYRSLCACGDR 
Sbjct: 366 YRVILSDVRDKLYNTRERARHLLASGFSEIPEEATFTDVEQFLEPLELCYRSLCACGDRS 425

Query: 427 IADGSLLDFLRQVSTFGLSLVRLDIRQESERHADVIDTITNYLGIGSYKEWSEEQKQEWL 486
           +ADGSLLDFLRQVSTFGLSLVRLDIRQES+RH DV+D IT YLGIGSY++W+EE++QEWL
Sbjct: 426 VADGSLLDFLRQVSTFGLSLVRLDIRQESDRHTDVMDAITEYLGIGSYRKWTEEKRQEWL 485

Query: 487 LSELSGKRPLFGPDLPKTEETSDVLDTFSVIAELPSDNFGAYIISMATAPSDVLAVELLQ 546
           LSEL+GKRPLFGPDLPK++E +DVLDTF V+AELPSD+FGAY+ISMATAPSDVLAVELLQ
Sbjct: 486 LSELNGKRPLFGPDLPKSDEIADVLDTFHVLAELPSDSFGAYVISMATAPSDVLAVELLQ 545

Query: 547 RECHVQKPLRVVPLFEKLADLEAAPSAMARLFSIDWYRNRIDGKQEVMIGYSDSGKDAGR 606
           RECHV+KPLRVVPLFEKLADLEAAP+A+ARLFS++WYRNRI+GKQEVMIGYSDSGKDAGR
Sbjct: 546 RECHVKKPLRVVPLFEKLADLEAAPAALARLFSVEWYRNRINGKQEVMIGYSDSGKDAGR 605

Query: 607 LSAAWQLYKAQEELIQVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHGSLRV 666
            SAAWQLYKAQEELI VAK YGVKLTMFHGRGGTVGRGGGPTHLAILSQPP+TIHGSLRV
Sbjct: 606 FSAAWQLYKAQEELINVAKLYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPETIHGSLRV 665

Query: 667 TVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMNPPVAPKPEWRALLDAMAVVATEKYRS 726
           TVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGM+PP++PKPEWRAL+D MA+VAT++YRS
Sbjct: 666 TVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMHPPISPKPEWRALMDEMAIVATKEYRS 725

Query: 727 IVFQEPRFVEYFRLVS 741
           IVF+EPRFVEYFRL +
Sbjct: 726 IVFEEPRFVEYFRLAT 741

BLAST of Cucsa.147540.2 vs. Swiss-Prot
Match: CAPP_SOLTU (Phosphoenolpyruvate carboxylase OS=Solanum tuberosum GN=PPC1 PE=2 SV=2)

HSP 1 Score: 1310.8 bits (3391), Expect = 0.0e+00
Identity = 647/738 (87.67%), Postives = 699/738 (94.72%), Query Frame = 1

Query: 5   KNLEKMASIDAQLRLLAPSKVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQECYEF 64
           +NL+K+ASIDAQLR L P+KVSEDDKLVEYDALLLDRFLDILQDLHGEDL+ETVQECYE 
Sbjct: 4   RNLDKLASIDAQLRQLVPAKVSEDDKLVEYDALLLDRFLDILQDLHGEDLKETVQECYEL 63

Query: 65  AAEYERKRDPEKLEELGNALTSLDPGDSIVVAKSFSHMLSLANLAEEVQIAYRRRIKLKK 124
           +AEYE K DP+KLEELGN LTSLDPGDSIV+AK+FSHML+LANLAEEVQIAYRRR KLKK
Sbjct: 64  SAEYEAKHDPKKLEELGNVLTSLDPGDSIVIAKAFSHMLNLANLAEEVQIAYRRRQKLKK 123

Query: 125 -GGFADEANATTESDIEETFKRLL-QLNKSPQEVFDALKNQTVDLVFTAHPTQSVRRSLL 184
            G F DE+NATTESDIEETFK+L+  L KSPQEVFDA+KNQTVDLV TAHPTQSVRRSLL
Sbjct: 124 KGDFGDESNATTESDIEETFKKLVGDLKKSPQEVFDAIKNQTVDLVLTAHPTQSVRRSLL 183

Query: 185 QKHGRIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMSY 244
           QKHGRIR+CL QLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMSY
Sbjct: 184 QKHGRIRDCLAQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMSY 243

Query: 245 FHETIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRDV 304
           FHETIWKGVPKFLRRVDTALKNIGI+ERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRDV
Sbjct: 244 FHETIWKGVPKFLRRVDTALKNIGINERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRDV 303

Query: 305 CLLARMMAANLYFSQIEDLMFELSMWRCSSELQTRAEELHRSSRKDAKHYIEFWKQIPPN 364
           CLLARMMAANLY+SQIEDLMFELSMWRC+ EL+ RA++L RSSR+D KHYIEFWKQ+PPN
Sbjct: 304 CLLARMMAANLYYSQIEDLMFELSMWRCNEELRVRADDLQRSSRRDEKHYIEFWKQVPPN 363

Query: 365 EPYRVILGDVRDKLYNTRERARHLLSSGMSEIPEDTTFTDVEQFLEPLELCYRSLCACGD 424
           EPYRVILGDVRDKLY TRERAR LL  G SEIPE+ T+T++EQFLEPLELCYRSLCACGD
Sbjct: 364 EPYRVILGDVRDKLYQTRERARQLLGHGYSEIPEEATYTNIEQFLEPLELCYRSLCACGD 423

Query: 425 RPIADGSLLDFLRQVSTFGLSLVRLDIRQESERHADVIDTITNYLGIGSYKEWSEEQKQE 484
             IADGSLLDFLRQVSTFGLSLVRLDIRQES+RH DV+D IT +L IGSY++WSEE++QE
Sbjct: 424 LSIADGSLLDFLRQVSTFGLSLVRLDIRQESDRHTDVLDAITQHLEIGSYRDWSEERRQE 483

Query: 485 WLLSELSGKRPLFGPDLPKTEETSDVLDTFSVIAELPSDNFGAYIISMATAPSDVLAVEL 544
           WLLSELSGKRPLFGPDLPKTEE +DVLDTF VIAELP+D FGAYIISMATAPSDVLAVEL
Sbjct: 484 WLLSELSGKRPLFGPDLPKTEEIADVLDTFHVIAELPADCFGAYIISMATAPSDVLAVEL 543

Query: 545 LQRECHVQKPLRVVPLFEKLADLEAAPSAMARLFSIDWYRNRIDGKQEVMIGYSDSGKDA 604
           LQREC V++PLRVVPLFEKLADL+AAP+A+ARLFSI+WYRNRI+GKQEVMIGYSDSGKDA
Sbjct: 544 LQRECRVRQPLRVVPLFEKLADLDAAPAAVARLFSIEWYRNRINGKQEVMIGYSDSGKDA 603

Query: 605 GRLSAAWQLYKAQEELIQVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHGSL 664
           GRLSAAWQLYKAQEELIQVAK++ VKLTMFHGRGGTVGRGGGP HLAILSQPP+TIHGSL
Sbjct: 604 GRLSAAWQLYKAQEELIQVAKEFDVKLTMFHGRGGTVGRGGGPAHLAILSQPPETIHGSL 663

Query: 665 RVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMNPPVAPKPEWRALLDAMAVVATEKY 724
           RVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGM+PPV+PKPEWRAL+D +AVVATEKY
Sbjct: 664 RVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMHPPVSPKPEWRALMDEIAVVATEKY 723

Query: 725 RSIVFQEPRFVEYFRLVS 741
           RSIVF+EPRFVEYFRL +
Sbjct: 724 RSIVFKEPRFVEYFRLAT 741

BLAST of Cucsa.147540.2 vs. TrEMBL
Match: J9XXT8_CUCSA (Phosphoenolpyruvate carboxylase 1 OS=Cucumis sativus GN=ppc2 PE=2 SV=1)

HSP 1 Score: 1472.2 bits (3810), Expect = 0.0e+00
Identity = 738/740 (99.73%), Postives = 739/740 (99.86%), Query Frame = 1

Query: 1   MAAVKNLEKMASIDAQLRLLAPSKVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQE 60
           MAAVKNLEKMASIDAQLRLLAPSKVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQE
Sbjct: 1   MAAVKNLEKMASIDAQLRLLAPSKVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQE 60

Query: 61  CYEFAAEYERKRDPEKLEELGNALTSLDPGDSIVVAKSFSHMLSLANLAEEVQIAYRRRI 120
           CYEFAAEYERKRDPEKLEELGNALTSLDPGDSIVVAKSFSHMLSLANLAEEVQIAYRRRI
Sbjct: 61  CYEFAAEYERKRDPEKLEELGNALTSLDPGDSIVVAKSFSHMLSLANLAEEVQIAYRRRI 120

Query: 121 KLKKGGFADEANATTESDIEETFKRLLQLNKSPQEVFDALKNQTVDLVFTAHPTQSVRRS 180
           KLKKGGFADEANATTESDIEETFKRLLQLNKSPQEVFDALKNQTVDLVFTAHPTQSVRRS
Sbjct: 121 KLKKGGFADEANATTESDIEETFKRLLQLNKSPQEVFDALKNQTVDLVFTAHPTQSVRRS 180

Query: 181 LLQKHGRIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGM 240
           LLQKHGRIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGM
Sbjct: 181 LLQKHGRIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGM 240

Query: 241 SYFHETIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTR 300
           SYFHETIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTR
Sbjct: 241 SYFHETIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTR 300

Query: 301 DVCLLARMMAANLYFSQIEDLMFELSMWRCSSELQTRAEELHRSSRKDAKHYIEFWKQIP 360
           DVCLLARMMAANLYFSQIEDLMFELSMWRCSSELQTRAEELHRSSRKDAKHYIEFWKQIP
Sbjct: 301 DVCLLARMMAANLYFSQIEDLMFELSMWRCSSELQTRAEELHRSSRKDAKHYIEFWKQIP 360

Query: 361 PNEPYRVILGDVRDKLYNTRERARHLLSSGMSEIPEDTTFTDVEQFLEPLELCYRSLCAC 420
           PNEPYRVILGDVRDKLYNTRERARHLLSSGMSEIPEDTTFTDVEQFLEPLELCYRSLCAC
Sbjct: 361 PNEPYRVILGDVRDKLYNTRERARHLLSSGMSEIPEDTTFTDVEQFLEPLELCYRSLCAC 420

Query: 421 GDRPIADGSLLDFLRQVSTFGLSLVRLDIRQESERHADVIDTITNYLGIGSYKEWSEEQK 480
           GDRPIADGSLLDFLRQVSTFGLSLVRLDIRQESERHADVIDTITNYLGIGSYKEWSEEQK
Sbjct: 421 GDRPIADGSLLDFLRQVSTFGLSLVRLDIRQESERHADVIDTITNYLGIGSYKEWSEEQK 480

Query: 481 QEWLLSELSGKRPLFGPDLPKTEETSDVLDTFSVIAELPSDNFGAYIISMATAPSDVLAV 540
           QEWLLSELSGKRPLFGPDLPKTEETSDVLDTFSVIAELPSDNFGAYIISMATAPSDVLAV
Sbjct: 481 QEWLLSELSGKRPLFGPDLPKTEETSDVLDTFSVIAELPSDNFGAYIISMATAPSDVLAV 540

Query: 541 ELLQRECHVQKPLRVVPLFEKLADLEAAPSAMARLFSIDWYRNRIDGKQEVMIGYSDSGK 600
           ELLQRECHVQKPLRVVPLFEKLADLEAAPSAMARLFSIDWYRNRIDGKQEVMIGYSDSGK
Sbjct: 541 ELLQRECHVQKPLRVVPLFEKLADLEAAPSAMARLFSIDWYRNRIDGKQEVMIGYSDSGK 600

Query: 601 DAGRLSAAWQLYKAQEELIQVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHG 660
           DAGRLSAAWQLYKAQEELIQVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHG
Sbjct: 601 DAGRLSAAWQLYKAQEELIQVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHG 660

Query: 661 SLRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMNPPVAPKPEWRALLDAMAVVATE 720
           SLRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMNPPVAPKPEWRALLDAMAVVATE
Sbjct: 661 SLRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMNPPVAPKPEWRALLDAMAVVATE 720

Query: 721 KYRSIVFQEPRFVEYFRLVS 741
           KYRSIVFQEPRFVEYFRL +
Sbjct: 721 KYRSIVFQEPRFVEYFRLAT 740

BLAST of Cucsa.147540.2 vs. TrEMBL
Match: A0A067KMT6_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_09795 PE=3 SV=1)

HSP 1 Score: 1348.6 bits (3489), Expect = 0.0e+00
Identity = 664/739 (89.85%), Postives = 708/739 (95.81%), Query Frame = 1

Query: 3   AVKNLEKMASIDAQLRLLAPSKVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQECY 62
           A KNLEKM SIDAQLRLLAP KVSEDDKLVEYDALLLDRFLDILQDLHGED+RETVQECY
Sbjct: 2   AGKNLEKMVSIDAQLRLLAPRKVSEDDKLVEYDALLLDRFLDILQDLHGEDIRETVQECY 61

Query: 63  EFAAEYERKRDPEKLEELGNALTSLDPGDSIVVAKSFSHMLSLANLAEEVQIAYRRRIKL 122
           E +AEYERK DP+KLEELG  LTSLDPGDSIVV KSFSHML+LANLAEEVQIAYRRR K 
Sbjct: 62  EISAEYERKHDPQKLEELGKVLTSLDPGDSIVVTKSFSHMLNLANLAEEVQIAYRRRTKS 121

Query: 123 KKGGFADEANATTESDIEETFKRLL-QLNKSPQEVFDALKNQTVDLVFTAHPTQSVRRSL 182
           KKG FADE +ATTESD+EETFK+L+ QL KSP+EVFDALKNQTVDLV TAHPTQSVRRSL
Sbjct: 122 KKGDFADENSATTESDMEETFKKLVVQLKKSPEEVFDALKNQTVDLVLTAHPTQSVRRSL 181

Query: 183 LQKHGRIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMS 242
           LQKH RIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMS
Sbjct: 182 LQKHARIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMS 241

Query: 243 YFHETIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRD 302
           YFHETIWKGVPKFLRRVDTALKNIGI+ER+PYNAPLIQFSSWMGGDRDGNPRVTPEVTRD
Sbjct: 242 YFHETIWKGVPKFLRRVDTALKNIGINERLPYNAPLIQFSSWMGGDRDGNPRVTPEVTRD 301

Query: 303 VCLLARMMAANLYFSQIEDLMFELSMWRCSSELQTRAEELHRSSRKDAKHYIEFWKQIPP 362
           VCLLARMMAANLYFSQIEDLMFELSMWRC+ EL+ RA+ LHRSSRKDAKHYIEFWKQIPP
Sbjct: 302 VCLLARMMAANLYFSQIEDLMFELSMWRCNEELRFRADALHRSSRKDAKHYIEFWKQIPP 361

Query: 363 NEPYRVILGDVRDKLYNTRERARHLLSSGMSEIPEDTTFTDVEQFLEPLELCYRSLCACG 422
           NEPYRVILGDVRDKLYNTRER+RHLL++G+S+IPE+ TFT+VEQFLEPLELCYRSLCACG
Sbjct: 362 NEPYRVILGDVRDKLYNTRERSRHLLTNGISDIPEEATFTNVEQFLEPLELCYRSLCACG 421

Query: 423 DRPIADGSLLDFLRQVSTFGLSLVRLDIRQESERHADVIDTITNYLGIGSYKEWSEEQKQ 482
           DRPIADGSLLDFLRQVSTFGLSLVRLDIRQES+RH DV+D IT +LGIGSY+EWSEE++Q
Sbjct: 422 DRPIADGSLLDFLRQVSTFGLSLVRLDIRQESDRHTDVLDAITKHLGIGSYREWSEERRQ 481

Query: 483 EWLLSELSGKRPLFGPDLPKTEETSDVLDTFSVIAELPSDNFGAYIISMATAPSDVLAVE 542
           EWLLSEL GKRPLFGPDLPKTEE +DVLDTF VIAELP DNFGAYIISMATAPSDVLAVE
Sbjct: 482 EWLLSELRGKRPLFGPDLPKTEEIADVLDTFHVIAELPPDNFGAYIISMATAPSDVLAVE 541

Query: 543 LLQRECHVQKPLRVVPLFEKLADLEAAPSAMARLFSIDWYRNRIDGKQEVMIGYSDSGKD 602
           LLQREC V++PLRVVPLFEKLADLEAAP+A++RLFSIDWYRNRI+GKQEVMIGYSDSGKD
Sbjct: 542 LLQRECRVKQPLRVVPLFEKLADLEAAPAAVSRLFSIDWYRNRINGKQEVMIGYSDSGKD 601

Query: 603 AGRLSAAWQLYKAQEELIQVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHGS 662
           AGRLSAAWQLYKAQEEL++VAKQYGVKL MFHGRGGTVGRGGGPTHLAILSQPPDTIHGS
Sbjct: 602 AGRLSAAWQLYKAQEELVKVAKQYGVKLVMFHGRGGTVGRGGGPTHLAILSQPPDTIHGS 661

Query: 663 LRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMNPPVAPKPEWRALLDAMAVVATEK 722
           LRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGM+PP++PKPEWRALLD MA++AT++
Sbjct: 662 LRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMHPPISPKPEWRALLDEMAIIATKE 721

Query: 723 YRSIVFQEPRFVEYFRLVS 741
           YRSIVFQEPRFVEYFRL +
Sbjct: 722 YRSIVFQEPRFVEYFRLAT 740

BLAST of Cucsa.147540.2 vs. TrEMBL
Match: A0A061F8Q3_THECC (Phosphoenolpyruvate carboxylase 3 isoform 1 OS=Theobroma cacao GN=TCM_031773 PE=3 SV=1)

HSP 1 Score: 1348.2 bits (3488), Expect = 0.0e+00
Identity = 664/735 (90.34%), Postives = 708/735 (96.33%), Query Frame = 1

Query: 7   LEKMASIDAQLRLLAPSKVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQECYEFAA 66
           LEK+ASIDAQLRLL PSKVSEDDKLVEYDALLLDRFLDILQDLHGEDL+ETVQECYE +A
Sbjct: 13  LEKLASIDAQLRLLVPSKVSEDDKLVEYDALLLDRFLDILQDLHGEDLKETVQECYELSA 72

Query: 67  EYERKRDPEKLEELGNALTSLDPGDSIVVAKSFSHMLSLANLAEEVQIAYRRRIKLKKGG 126
           EYE K +P+KLEELGN LTSLDPGDSIVVAK+FSHML+LANLAEEVQIAYRRRIKLKKG 
Sbjct: 73  EYEGKNNPKKLEELGNVLTSLDPGDSIVVAKAFSHMLNLANLAEEVQIAYRRRIKLKKGD 132

Query: 127 FADEANATTESDIEETFKRLL-QLNKSPQEVFDALKNQTVDLVFTAHPTQSVRRSLLQKH 186
           F DE +ATTESDIEET KRL+  L KSP+EVFDALKNQTVDLVFTAHPTQSVRRSLLQKH
Sbjct: 133 FIDENSATTESDIEETLKRLVFDLKKSPEEVFDALKNQTVDLVFTAHPTQSVRRSLLQKH 192

Query: 187 GRIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMSYFHE 246
           GRIRNCL QLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMSYFHE
Sbjct: 193 GRIRNCLAQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMSYFHE 252

Query: 247 TIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRDVCLL 306
           T+WKGVPKFLRRVDTALKNIGI+ERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRDVCLL
Sbjct: 253 TVWKGVPKFLRRVDTALKNIGINERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRDVCLL 312

Query: 307 ARMMAANLYFSQIEDLMFELSMWRCSSELQTRAEELHRSSRKDAKHYIEFWKQIPPNEPY 366
           ARMMAANLY+SQIEDLMFELSMWRCS EL+ RA+ELHRSSR+DAKHYIEFWKQIPPNEPY
Sbjct: 313 ARMMAANLYYSQIEDLMFELSMWRCSDELRVRADELHRSSRRDAKHYIEFWKQIPPNEPY 372

Query: 367 RVILGDVRDKLYNTRERARHLLSSGMSEIPEDTTFTDVEQFLEPLELCYRSLCACGDRPI 426
           RVILGDVRDKLY TRER+R +LS G+S+IPE+ TFT++EQFLEPLELCYRSLC+CGDRPI
Sbjct: 373 RVILGDVRDKLYQTRERSRQMLSHGISDIPEEATFTNIEQFLEPLELCYRSLCSCGDRPI 432

Query: 427 ADGSLLDFLRQVSTFGLSLVRLDIRQESERHADVIDTITNYLGIGSYKEWSEEQKQEWLL 486
           ADGSLLDFLRQVSTFGLSLVRLDIRQES+RH DVID IT +L +GSY+EWSEE+KQEWLL
Sbjct: 433 ADGSLLDFLRQVSTFGLSLVRLDIRQESDRHTDVIDAITKHLELGSYREWSEERKQEWLL 492

Query: 487 SELSGKRPLFGPDLPKTEETSDVLDTFSVIAELPSDNFGAYIISMATAPSDVLAVELLQR 546
           SELSGKRPLFGPDLPKTEE +DVLDTF VIAELP+DNFGAYIISMATAPSDVLAVELLQR
Sbjct: 493 SELSGKRPLFGPDLPKTEEIADVLDTFHVIAELPADNFGAYIISMATAPSDVLAVELLQR 552

Query: 547 ECHVQKPLRVVPLFEKLADLEAAPSAMARLFSIDWYRNRIDGKQEVMIGYSDSGKDAGRL 606
           ECHV++PLRVVPLFEKLADLEAAP+A+ARLFSIDWYRNRI+GKQEVMIGYSDSGKDAGRL
Sbjct: 553 ECHVKEPLRVVPLFEKLADLEAAPAALARLFSIDWYRNRINGKQEVMIGYSDSGKDAGRL 612

Query: 607 SAAWQLYKAQEELIQVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHGSLRVT 666
           SAAWQLYKAQEELI+VAKQ+GVKLTMFHGRGGTVGRGGGPTHLAILSQPP+TIHGSLRVT
Sbjct: 613 SAAWQLYKAQEELIKVAKQFGVKLTMFHGRGGTVGRGGGPTHLAILSQPPETIHGSLRVT 672

Query: 667 VQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMNPPVAPKPEWRALLDAMAVVATEKYRSI 726
           VQGEVIEQSFGEEHLCFRTLQRFTAATLEHGM+PPV+PKPEWRAL+D MAV+ATEKYRSI
Sbjct: 673 VQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMHPPVSPKPEWRALMDEMAVIATEKYRSI 732

Query: 727 VFQEPRFVEYFRLVS 741
           VFQEPRFVEYFRL +
Sbjct: 733 VFQEPRFVEYFRLAT 747

BLAST of Cucsa.147540.2 vs. TrEMBL
Match: U5HTH6_MANES (Phosphoenolpyruvate carboxylase OS=Manihot esculenta subsp. flabellifolia GN=PEPC PE=2 SV=1)

HSP 1 Score: 1347.4 bits (3486), Expect = 0.0e+00
Identity = 662/739 (89.58%), Postives = 709/739 (95.94%), Query Frame = 1

Query: 3   AVKNLEKMASIDAQLRLLAPSKVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQECY 62
           A +NLEKMASIDAQLRLLAP KVSEDDKLVEYDALLLDRFLDILQDLHGE++RETVQ+CY
Sbjct: 2   AARNLEKMASIDAQLRLLAPRKVSEDDKLVEYDALLLDRFLDILQDLHGEEIRETVQDCY 61

Query: 63  EFAAEYERKRDPEKLEELGNALTSLDPGDSIVVAKSFSHMLSLANLAEEVQIAYRRRIKL 122
           E +AEYE K DP+KLEELG  LTSLDPGDSIVVAKSFSHML+LANLAEEVQIAYRRRIKL
Sbjct: 62  ELSAEYEGKHDPQKLEELGKVLTSLDPGDSIVVAKSFSHMLNLANLAEEVQIAYRRRIKL 121

Query: 123 KKGGFADEANATTESDIEETFKRLL-QLNKSPQEVFDALKNQTVDLVFTAHPTQSVRRSL 182
           KKG FADE +ATTESDIE+T KRL+ QL KSP+EVFDALKNQTVDLV TAHPTQSVRRSL
Sbjct: 122 KKGDFADENSATTESDIEKTLKRLVVQLKKSPEEVFDALKNQTVDLVLTAHPTQSVRRSL 181

Query: 183 LQKHGRIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMS 242
           LQKH RIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMS
Sbjct: 182 LQKHARIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMS 241

Query: 243 YFHETIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRD 302
           YFHETIWKGVPKFLRRVDTALKNIGI+ER+PYNAPLIQFSSWMGGDRDGNPRVTPEVTRD
Sbjct: 242 YFHETIWKGVPKFLRRVDTALKNIGINERIPYNAPLIQFSSWMGGDRDGNPRVTPEVTRD 301

Query: 303 VCLLARMMAANLYFSQIEDLMFELSMWRCSSELQTRAEELHRSSRKDAKHYIEFWKQIPP 362
           VCLLARMMAAN YFSQIEDLMFELSMWRC+ EL+  A+ELHRSSR+DAKHYIEFWKQIP 
Sbjct: 302 VCLLARMMAANSYFSQIEDLMFELSMWRCNDELRVHADELHRSSRRDAKHYIEFWKQIPA 361

Query: 363 NEPYRVILGDVRDKLYNTRERARHLLSSGMSEIPEDTTFTDVEQFLEPLELCYRSLCACG 422
           +EPYRVILGDVRDKLYNTRER+RHLL +G+S+IPE+ TFT+VEQFL+PLELCYRSLCACG
Sbjct: 362 SEPYRVILGDVRDKLYNTRERSRHLLVNGISDIPEEATFTNVEQFLQPLELCYRSLCACG 421

Query: 423 DRPIADGSLLDFLRQVSTFGLSLVRLDIRQESERHADVIDTITNYLGIGSYKEWSEEQKQ 482
           DRPIADGSLLDFLRQVSTFGLSLVRLDIRQES+RH DV+D IT YLGIGSY+EWSEE++Q
Sbjct: 422 DRPIADGSLLDFLRQVSTFGLSLVRLDIRQESDRHTDVLDAITKYLGIGSYREWSEERRQ 481

Query: 483 EWLLSELSGKRPLFGPDLPKTEETSDVLDTFSVIAELPSDNFGAYIISMATAPSDVLAVE 542
           EWLLSEL GKRPLFGPDLPKTEE +DVLDTF VIAELP DNFGAYIISMATAPSDVLAVE
Sbjct: 482 EWLLSELRGKRPLFGPDLPKTEEIADVLDTFHVIAELPPDNFGAYIISMATAPSDVLAVE 541

Query: 543 LLQRECHVQKPLRVVPLFEKLADLEAAPSAMARLFSIDWYRNRIDGKQEVMIGYSDSGKD 602
           LLQRECHV++PLRVVPLFEKLADLEAAP+A+ARLFS+DWYRNRI+GKQEVMIGYSDSGKD
Sbjct: 542 LLQRECHVKQPLRVVPLFEKLADLEAAPAAVARLFSVDWYRNRINGKQEVMIGYSDSGKD 601

Query: 603 AGRLSAAWQLYKAQEELIQVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHGS 662
           AGRLSAAWQLYKAQEEL++VAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHGS
Sbjct: 602 AGRLSAAWQLYKAQEELVKVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHGS 661

Query: 663 LRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMNPPVAPKPEWRALLDAMAVVATEK 722
           LRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGM+PPV+PKPEWRAL+D MA++AT++
Sbjct: 662 LRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMHPPVSPKPEWRALMDEMAIIATKE 721

Query: 723 YRSIVFQEPRFVEYFRLVS 741
           YRSIVFQEPRFVEYFRL +
Sbjct: 722 YRSIVFQEPRFVEYFRLAT 740

BLAST of Cucsa.147540.2 vs. TrEMBL
Match: A7UH66_JATCU (Phosphoenolpyruvate carboxylase OS=Jatropha curcas PE=2 SV=1)

HSP 1 Score: 1345.9 bits (3482), Expect = 0.0e+00
Identity = 663/739 (89.72%), Postives = 707/739 (95.67%), Query Frame = 1

Query: 3   AVKNLEKMASIDAQLRLLAPSKVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQECY 62
           A KNLEKM SIDAQLRLLAP KVSEDDKLVEYDALLLDRFLDILQDLHGED+RETVQECY
Sbjct: 2   AGKNLEKMVSIDAQLRLLAPRKVSEDDKLVEYDALLLDRFLDILQDLHGEDIRETVQECY 61

Query: 63  EFAAEYERKRDPEKLEELGNALTSLDPGDSIVVAKSFSHMLSLANLAEEVQIAYRRRIKL 122
           E +A YERK DP+KLEELG  LTSLDPGDSIVV KSFSHML+LANLAEEVQIAYRRR K 
Sbjct: 62  EISAGYERKHDPQKLEELGKVLTSLDPGDSIVVTKSFSHMLNLANLAEEVQIAYRRRTKS 121

Query: 123 KKGGFADEANATTESDIEETFKRLL-QLNKSPQEVFDALKNQTVDLVFTAHPTQSVRRSL 182
           KKG FADE +ATTESD+EETFK+L+ QL KSP+EVFDALKNQTVDLV TAHPTQSVRRSL
Sbjct: 122 KKGDFADENSATTESDMEETFKKLVVQLKKSPEEVFDALKNQTVDLVLTAHPTQSVRRSL 181

Query: 183 LQKHGRIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMS 242
           LQKH RIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMS
Sbjct: 182 LQKHARIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMS 241

Query: 243 YFHETIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRD 302
           YFHETIWKGVPKFLRRVDTALKNIGI+ER+PYNAPLIQFSSWMGGDRDGNPRVTPEVTRD
Sbjct: 242 YFHETIWKGVPKFLRRVDTALKNIGINERLPYNAPLIQFSSWMGGDRDGNPRVTPEVTRD 301

Query: 303 VCLLARMMAANLYFSQIEDLMFELSMWRCSSELQTRAEELHRSSRKDAKHYIEFWKQIPP 362
           VCLLARMMAANLYFSQIEDLMFELSMWRC+ EL+ RA+ LHRSSRKDAKHYIEFWKQIPP
Sbjct: 302 VCLLARMMAANLYFSQIEDLMFELSMWRCNEELRFRADALHRSSRKDAKHYIEFWKQIPP 361

Query: 363 NEPYRVILGDVRDKLYNTRERARHLLSSGMSEIPEDTTFTDVEQFLEPLELCYRSLCACG 422
           NEPYRVILGDVRDKLYNTRER+RHLL++G+S+IPE+ TFT+VEQFLEPLELCYRSLCACG
Sbjct: 362 NEPYRVILGDVRDKLYNTRERSRHLLTNGISDIPEEATFTNVEQFLEPLELCYRSLCACG 421

Query: 423 DRPIADGSLLDFLRQVSTFGLSLVRLDIRQESERHADVIDTITNYLGIGSYKEWSEEQKQ 482
           DRPIADGSLLDFLRQVSTFGLSLVRLDIRQES+RH DV+D IT +LGIGSY+EWSEE++Q
Sbjct: 422 DRPIADGSLLDFLRQVSTFGLSLVRLDIRQESDRHTDVLDAITKHLGIGSYREWSEERRQ 481

Query: 483 EWLLSELSGKRPLFGPDLPKTEETSDVLDTFSVIAELPSDNFGAYIISMATAPSDVLAVE 542
           EWLLSEL GKRPLFGPDLPKTEE +DVLDTF VIAELP DNFGAYIISMATAPSDVLAVE
Sbjct: 482 EWLLSELRGKRPLFGPDLPKTEEIADVLDTFHVIAELPPDNFGAYIISMATAPSDVLAVE 541

Query: 543 LLQRECHVQKPLRVVPLFEKLADLEAAPSAMARLFSIDWYRNRIDGKQEVMIGYSDSGKD 602
           LLQREC V++PLRVVPLFEKLADLEAAP+A++RLFSIDWYRNRI+GKQEVMIGYSDSGKD
Sbjct: 542 LLQRECRVKQPLRVVPLFEKLADLEAAPAAVSRLFSIDWYRNRINGKQEVMIGYSDSGKD 601

Query: 603 AGRLSAAWQLYKAQEELIQVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHGS 662
           AGRLSAAWQLYKAQEEL++VAKQYGVKL MFHGRGGTVGRGGGPTHLAILSQPPDTIHGS
Sbjct: 602 AGRLSAAWQLYKAQEELVKVAKQYGVKLVMFHGRGGTVGRGGGPTHLAILSQPPDTIHGS 661

Query: 663 LRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMNPPVAPKPEWRALLDAMAVVATEK 722
           LRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGM+PP++PKPEWRALLD MA++AT++
Sbjct: 662 LRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMHPPISPKPEWRALLDEMAIIATKE 721

Query: 723 YRSIVFQEPRFVEYFRLVS 741
           YRSIVFQEPRFVEYFRL +
Sbjct: 722 YRSIVFQEPRFVEYFRLAT 740

BLAST of Cucsa.147540.2 vs. TAIR10
Match: AT2G42600.2 (AT2G42600.2 phosphoenolpyruvate carboxylase 2)

HSP 1 Score: 1316.2 bits (3405), Expect = 0.0e+00
Identity = 649/739 (87.82%), Postives = 700/739 (94.72%), Query Frame = 1

Query: 3   AVKNLEKMASIDAQLRLLAPSKVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQECY 62
           A +NLEKMASIDAQLRLLAP KVSEDDKL+EYDALLLDRFLDILQDLHGED+RE VQECY
Sbjct: 2   AARNLEKMASIDAQLRLLAPGKVSEDDKLIEYDALLLDRFLDILQDLHGEDVREFVQECY 61

Query: 63  EFAAEYERKRDPEKLEELGNALTSLDPGDSIVVAKSFSHMLSLANLAEEVQIAYRRRIK- 122
           E AA+Y+  R+ EKLEELGN LTSLDPGDSIVV KSFS+MLSLANLAEEVQIAYRRRIK 
Sbjct: 62  EVAADYDGNRNTEKLEELGNMLTSLDPGDSIVVTKSFSNMLSLANLAEEVQIAYRRRIKK 121

Query: 123 LKKGGFADEANATTESDIEETFKRLLQLNKSPQEVFDALKNQTVDLVFTAHPTQSVRRSL 182
           LKKG FADEA+ATTESDIEET KRLLQLNK+P+EVFDALKNQTVDLV TAHPTQSVRRSL
Sbjct: 122 LKKGDFADEASATTESDIEETLKRLLQLNKTPEEVFDALKNQTVDLVLTAHPTQSVRRSL 181

Query: 183 LQKHGRIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMS 242
           LQK GRIR+CLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMS
Sbjct: 182 LQKFGRIRDCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMS 241

Query: 243 YFHETIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRD 302
           YFHETIWKGVPKFLRRVDTALKNIGI+ERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRD
Sbjct: 242 YFHETIWKGVPKFLRRVDTALKNIGINERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRD 301

Query: 303 VCLLARMMAANLYFSQIEDLMFELSMWRCSSELQTRAEELHRSSRKDAKHYIEFWKQIPP 362
           VCLLARMMAANLYFSQIEDLMFE+SMWRC+ EL+ RAE   R +++DAKHYIEFWKQIP 
Sbjct: 302 VCLLARMMAANLYFSQIEDLMFEMSMWRCNEELRVRAER-QRCAKRDAKHYIEFWKQIPA 361

Query: 363 NEPYRVILGDVRDKLYNTRERARHLLSSGMSEIPEDTTFTDVEQFLEPLELCYRSLCACG 422
           NEPYR ILGDVRDKLYNTRERAR LLSSG+S++PED  FT V+QFLEPLELCYRSLC CG
Sbjct: 362 NEPYRAILGDVRDKLYNTRERARQLLSSGVSDVPEDAVFTSVDQFLEPLELCYRSLCDCG 421

Query: 423 DRPIADGSLLDFLRQVSTFGLSLVRLDIRQESERHADVIDTITNYLGIGSYKEWSEEQKQ 482
           DRPIADGSLLDFLRQVSTFGL+LV+LDIRQESERH+DV+D IT +LGIGSYKEWSE+++Q
Sbjct: 422 DRPIADGSLLDFLRQVSTFGLALVKLDIRQESERHSDVLDAITTHLGIGSYKEWSEDKRQ 481

Query: 483 EWLLSELSGKRPLFGPDLPKTEETSDVLDTFSVIAELPSDNFGAYIISMATAPSDVLAVE 542
           EWLLSELSGKRPLFGPDLPKTEE +DVLDTF VI+ELPSD+FGAYIISMATAPSDVLAVE
Sbjct: 482 EWLLSELSGKRPLFGPDLPKTEEVADVLDTFKVISELPSDSFGAYIISMATAPSDVLAVE 541

Query: 543 LLQRECHVQKPLRVVPLFEKLADLEAAPSAMARLFSIDWYRNRIDGKQEVMIGYSDSGKD 602
           LLQREC +  PLRVVPLFEKLADLE+AP+A+ARLFSI+WYRNRI+GKQEVMIGYSDSGKD
Sbjct: 542 LLQRECGITDPLRVVPLFEKLADLESAPAAVARLFSIEWYRNRINGKQEVMIGYSDSGKD 601

Query: 603 AGRLSAAWQLYKAQEELIQVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHGS 662
           AGRLSAAWQLYK QEEL++VAK+YGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHG 
Sbjct: 602 AGRLSAAWQLYKTQEELVKVAKEYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHGQ 661

Query: 663 LRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMNPPVAPKPEWRALLDAMAVVATEK 722
           LRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGM+PPV+PKPEWR L+D MA++ATE+
Sbjct: 662 LRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMHPPVSPKPEWRVLMDEMAIIATEE 721

Query: 723 YRSIVFQEPRFVEYFRLVS 741
           YRS+VF+EPRFVEYFRL +
Sbjct: 722 YRSVVFKEPRFVEYFRLAT 739

BLAST of Cucsa.147540.2 vs. TAIR10
Match: AT3G14940.1 (AT3G14940.1 phosphoenolpyruvate carboxylase 3)

HSP 1 Score: 1305.8 bits (3378), Expect = 0.0e+00
Identity = 647/742 (87.20%), Postives = 704/742 (94.88%), Query Frame = 1

Query: 3   AVKNLEKMASIDAQLRLLAPSKVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQECY 62
           A +N+EKMASIDAQLR L P+KVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQE Y
Sbjct: 2   AGRNIEKMASIDAQLRQLVPAKVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQELY 61

Query: 63  EFAAEYERKRDPEKLEELGNALTSLDPGDSIVVAKSFSHMLSLANLAEEVQIAYRRRIK- 122
           E +AEYE KR+P KLEELG+ LTSLDPGDSIV++K+FSHML+LANLAEEVQIA+RRRIK 
Sbjct: 62  ELSAEYEGKREPSKLEELGSVLTSLDPGDSIVISKAFSHMLNLANLAEEVQIAHRRRIKK 121

Query: 123 LKKGGFADEANATTESDIEETFKRLLQ-LNKSPQEVFDALKNQTVDLVFTAHPTQSVRRS 182
           LKKG F DE++ATTESDIEETFKRL+  L KSP+E+FDALKNQTVDLV TAHPTQSVRRS
Sbjct: 122 LKKGDFVDESSATTESDIEETFKRLVSDLGKSPEEIFDALKNQTVDLVLTAHPTQSVRRS 181

Query: 183 LLQKHGRIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGM 242
           LLQKHGRIR+CL QLYAKDITPDDKQELDE+LQREIQAAFRTDEIRRTPPTPQDEMRAGM
Sbjct: 182 LLQKHGRIRDCLAQLYAKDITPDDKQELDESLQREIQAAFRTDEIRRTPPTPQDEMRAGM 241

Query: 243 SYFHETIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTR 302
           SYFHETIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTR
Sbjct: 242 SYFHETIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTR 301

Query: 303 DVCLLARMMAANLYFSQIEDLMFELSMWRCSSELQTRAEELHRSSRKDA-KHYIEFWKQI 362
           DVCLLARMMAANLY++QIE+LMFELSMWRC+ E + RA+ELHR+SRKDA KHYIEFWK I
Sbjct: 302 DVCLLARMMAANLYYNQIENLMFELSMWRCTDEFRVRADELHRNSRKDAAKHYIEFWKTI 361

Query: 363 PPNEPYRVILGDVRDKLYNTRERARHLLSSGMSEIPEDTTFTDVEQFLEPLELCYRSLCA 422
           PP EPYRVILGDVRDKLY+TRER+R LLS+G+S+IPE+ TFT+VEQFLEPLELCYRSLC+
Sbjct: 362 PPTEPYRVILGDVRDKLYHTRERSRQLLSNGISDIPEEATFTNVEQFLEPLELCYRSLCS 421

Query: 423 CGDRPIADGSLLDFLRQVSTFGLSLVRLDIRQESERHADVIDTITNYLGIGS-YKEWSEE 482
           CGD PIADGSLLDFLRQVSTFGLSLVRLDIRQESERH DV+D IT +L IGS Y++WSEE
Sbjct: 422 CGDSPIADGSLLDFLRQVSTFGLSLVRLDIRQESERHTDVLDAITKHLDIGSSYRDWSEE 481

Query: 483 QKQEWLLSELSGKRPLFGPDLPKTEETSDVLDTFSVIAELPSDNFGAYIISMATAPSDVL 542
            +QEWLL+ELSGKRPLFGPDLPKTEE SDVLDTF VI+ELPSD FGAYIISMAT+PSDVL
Sbjct: 482 GRQEWLLAELSGKRPLFGPDLPKTEEISDVLDTFKVISELPSDCFGAYIISMATSPSDVL 541

Query: 543 AVELLQRECHVQKPLRVVPLFEKLADLEAAPSAMARLFSIDWYRNRIDGKQEVMIGYSDS 602
           AVELLQRECHV+ PLRVVPLFEKLADLEAAP+A+ARLFSIDWY+NRI+GKQEVMIGYSDS
Sbjct: 542 AVELLQRECHVKNPLRVVPLFEKLADLEAAPAAVARLFSIDWYKNRINGKQEVMIGYSDS 601

Query: 603 GKDAGRLSAAWQLYKAQEELIQVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTI 662
           GKDAGRLSAAW+LYKAQEEL++VAK+YGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDT+
Sbjct: 602 GKDAGRLSAAWELYKAQEELVKVAKKYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTV 661

Query: 663 HGSLRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMNPPVAPKPEWRALLDAMAVVA 722
           +GSLRVTVQGEVIEQSFGE HLCFRTLQRFTAATLEHGMNPP++PKPEWRALLD MAVVA
Sbjct: 662 NGSLRVTVQGEVIEQSFGEAHLCFRTLQRFTAATLEHGMNPPISPKPEWRALLDEMAVVA 721

Query: 723 TEKYRSIVFQEPRFVEYFRLVS 741
           TE+YRS+VFQEPRFVEYFRL +
Sbjct: 722 TEEYRSVVFQEPRFVEYFRLAT 743

BLAST of Cucsa.147540.2 vs. TAIR10
Match: AT1G53310.1 (AT1G53310.1 phosphoenolpyruvate carboxylase 1)

HSP 1 Score: 1292.7 bits (3344), Expect = 0.0e+00
Identity = 638/741 (86.10%), Postives = 697/741 (94.06%), Query Frame = 1

Query: 3   AVKNLEKMASIDAQLRLLAPSKVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQECY 62
           A + LEKMASID  LR L P KVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQE Y
Sbjct: 2   ANRKLEKMASIDVHLRQLVPGKVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQELY 61

Query: 63  EFAAEYERKRDPEKLEELGNALTSLDPGDSIVVAKSFSHMLSLANLAEEVQIAYRRRIK- 122
           E +AEYE K +P+KLEELG+ LTSLDPGDSIV+AK+FSHML+LANLAEEVQIAYRRRIK 
Sbjct: 62  EHSAEYEGKHEPKKLEELGSVLTSLDPGDSIVIAKAFSHMLNLANLAEEVQIAYRRRIKK 121

Query: 123 LKKGGFADEANATTESDIEETFKRLL-QLNKSPQEVFDALKNQTVDLVFTAHPTQSVRRS 182
           LKKG F DE++ATTESD+EETFK+L+  LNKSP+E+FDALKNQTVDLV TAHPTQSVRRS
Sbjct: 122 LKKGDFVDESSATTESDLEETFKKLVGDLNKSPEEIFDALKNQTVDLVLTAHPTQSVRRS 181

Query: 183 LLQKHGRIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGM 242
           LLQKHGRIR+CL QLYAKDITPDDKQELDEALQREIQAAFRTDEI+RTPPTPQDEMRAGM
Sbjct: 182 LLQKHGRIRDCLAQLYAKDITPDDKQELDEALQREIQAAFRTDEIKRTPPTPQDEMRAGM 241

Query: 243 SYFHETIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTR 302
           SYFHETIWKGVPKFLRRVDTALKNIGI+ERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTR
Sbjct: 242 SYFHETIWKGVPKFLRRVDTALKNIGIEERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTR 301

Query: 303 DVCLLARMMAANLYFSQIEDLMFELSMWRCSSELQTRAEELHRSSRKDA-KHYIEFWKQI 362
           DVCLLARMMAA +YF+QIEDLMFE+SMWRC+ EL+ RA+E+H +SRKDA KHYIEFWK I
Sbjct: 302 DVCLLARMMAATMYFNQIEDLMFEMSMWRCNDELRARADEVHANSRKDAAKHYIEFWKSI 361

Query: 363 PPNEPYRVILGDVRDKLYNTRERARHLLSSGMSEIPEDTTFTDVEQFLEPLELCYRSLCA 422
           P  EPYRVILGDVRDKLY+TRERA  LLS+G S++P + TF ++EQFLEPLELCYRSLC+
Sbjct: 362 PTTEPYRVILGDVRDKLYHTRERAHQLLSNGHSDVPVEATFINLEQFLEPLELCYRSLCS 421

Query: 423 CGDRPIADGSLLDFLRQVSTFGLSLVRLDIRQESERHADVIDTITNYLGIGSYKEWSEEQ 482
           CGDRPIADGSLLDFLRQVSTFGLSLVRLDIRQES+RH DV+D IT +L IGSY+EWSEE+
Sbjct: 422 CGDRPIADGSLLDFLRQVSTFGLSLVRLDIRQESDRHTDVLDAITTHLDIGSYREWSEER 481

Query: 483 KQEWLLSELSGKRPLFGPDLPKTEETSDVLDTFSVIAELPSDNFGAYIISMATAPSDVLA 542
           +QEWLLSELSGKRPLFG DLPKTEE +DVLDTF VIAELP+D+FGAYIISMATAPSDVLA
Sbjct: 482 RQEWLLSELSGKRPLFGSDLPKTEEIADVLDTFHVIAELPADSFGAYIISMATAPSDVLA 541

Query: 543 VELLQRECHVQKPLRVVPLFEKLADLEAAPSAMARLFSIDWYRNRIDGKQEVMIGYSDSG 602
           VELLQREC V++PLRVVPLFEKLADLEAAP+A+ARLFS+DWY+NRI+GKQEVMIGYSDSG
Sbjct: 542 VELLQRECRVKQPLRVVPLFEKLADLEAAPAAVARLFSVDWYKNRINGKQEVMIGYSDSG 601

Query: 603 KDAGRLSAAWQLYKAQEELIQVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIH 662
           KDAGRLSAAWQLYKAQEEL++VAK+YGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTI+
Sbjct: 602 KDAGRLSAAWQLYKAQEELVKVAKEYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIN 661

Query: 663 GSLRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMNPPVAPKPEWRALLDAMAVVAT 722
           GSLRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGM PP++PKPEWRALLD MAVVAT
Sbjct: 662 GSLRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMRPPISPKPEWRALLDEMAVVAT 721

Query: 723 EKYRSIVFQEPRFVEYFRLVS 741
           E+YRS+VFQEPRFVEYFRL +
Sbjct: 722 EEYRSVVFQEPRFVEYFRLAT 742

BLAST of Cucsa.147540.2 vs. TAIR10
Match: AT1G68750.1 (AT1G68750.1 phosphoenolpyruvate carboxylase 4)

HSP 1 Score: 376.7 bits (966), Expect = 3.3e-104
Identity = 192/389 (49.36%), Postives = 263/389 (67.61%), Query Frame = 1

Query: 364 PYRVILGDVRDKLYNTRERARHLLSSGMSEIPEDTTFTDVEQFLEPLELCYRSLCACGDR 423
           PYR++LG+V++KL  TR     L+     E     ++   +Q LEPL LCY SL + G R
Sbjct: 448 PYRIVLGEVKEKLVKTRRLLELLIEGLPCEYDPKNSYETSDQLLEPLLLCYESLQSSGAR 507

Query: 424 PIADGSLLDFLRQVSTFGLSLVRLDIRQESERHADVIDTITNYLGIGSYKEWSEEQKQEW 483
            +ADG L D +R+VSTFG+ LV+LD+RQE+ RH++ +D IT YL +G+Y EW EE+K E+
Sbjct: 508 VLADGRLADLIRRVSTFGMVLVKLDLRQEAARHSEALDAITTYLDMGTYSEWDEEKKLEF 567

Query: 484 LLSELSGKRPLFGPDLPKTEETSDVLDTFSVIAELPSDNFGAYIISMATAPSDVLAVELL 543
           L  EL GKRPL    +    +  +VLDTF V AEL S++ GAY+ISMA+  SDVLAVELL
Sbjct: 568 LTRELKGKRPLVPQCIKVGPDVKEVLDTFRVAAELGSESLGAYVISMASNASDVLAVELL 627

Query: 544 QRECHVQ------KP-----LRVVPLFEKLADLEAAPSAMARLFSIDWYRNRI----DGK 603
           Q++  +       KP     LRVVPLFE + DL AA  ++ +L SIDWYR  I    +G 
Sbjct: 628 QKDARLALTSEHGKPCPGGTLRVVPLFETVNDLRAAGPSIRKLLSIDWYREHIQKNHNGH 687

Query: 604 QEVMIGYSDSGKDAGRLSAAWQLYKAQEELIQVAKQYGVKLTMFHGRGGTVGRGGGPTHL 663
           QEVM+GYSDSGKDAGR +AAW+LYKAQE ++    ++G+K+T+FHGRGG++GRGGGPT+L
Sbjct: 688 QEVMVGYSDSGKDAGRFTAAWELYKAQENVVAACNEFGIKITLFHGRGGSIGRGGGPTYL 747

Query: 664 AILSQPPDTIHGSLRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMNPPVAPKPE-W 723
           AI SQPP ++ GSLR T QGE+++  FG      R L+ +T A L   + PP  P+ E W
Sbjct: 748 AIQSQPPGSVMGSLRSTEQGEMVQAKFGIPQTAVRQLEVYTTAVLLATLKPPQPPREEKW 807

Query: 724 RALLDAMAVVATEKYRSIVFQEPRFVEYF 737
           R L++ ++ ++ + YRS V++ P F+ YF
Sbjct: 808 RNLMEEISGISCQHYRSTVYENPEFLSYF 836

HSP 2 Score: 183.7 bits (465), Expect = 4.1e-46
Identity = 113/313 (36.10%), Postives = 176/313 (56.23%), Query Frame = 1

Query: 37  LLLDRFLDILQDLHGEDLRETVQECYEFAAEYERKR-----DPEKL--EELGNALTSLDP 96
           LL   F D+LQ   G    E V+     A      R     D   L  ++L + ++ +  
Sbjct: 23  LLGSLFHDVLQREVGNPFMEKVERIRILAQSALNLRMAGIEDTANLLEKQLTSEISKMPL 82

Query: 97  GDSIVVAKSFSHMLSLANLAEEVQIAYRRRIKLKKGGFADEANATTES-DIEETFKRLLQ 156
            +++ +A++F+H L+L  +A+     + R  K+         N T  +   ++ F +LLQ
Sbjct: 83  EEALTLARTFTHSLNLMGIAD----THHRMHKVH--------NVTQLARSCDDIFSQLLQ 142

Query: 157 LNKSPQEVFDALKNQTVDLVFTAHPTQSVRRSLLQKHGRIRNCLTQLYAKDITPDDKQEL 216
              SP E++  +  Q V++V TAHPTQ  RR+L  KH RI + L      D++ +D++ L
Sbjct: 143 SGISPDELYKTVCKQEVEIVLTAHPTQINRRTLQYKHIRIAHLLEYNTRSDLSVEDRETL 202

Query: 217 DEALQREIQAAFRTDEIRRTPPTPQDEMRAGMSYFHETIWKGVPKFLRRVDTALKNIGID 276
            E L REI + ++TDE+RR  PTP DE RAG++   +++WK VP++LRRV  +LK     
Sbjct: 203 IEDLVREITSLWQTDELRRQKPTPVDEARAGLNIVEQSLWKAVPQYLRRVSNSLKKF-TG 262

Query: 277 ERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRDVCLLARMMAANLYFSQIEDLMFELSMW 336
           + +P     ++F SWMGGDRDGNP VT +VT++V LL+R MA +LY  +++ L FELS  
Sbjct: 263 KPLPLTCTPMKFGSWMGGDRDGNPNVTAKVTKEVSLLSRWMAIDLYIREVDSLRFELSTD 322

Query: 337 RCSSELQTRAEEL 342
           RCS      A+++
Sbjct: 323 RCSDRFSRLADKI 322

BLAST of Cucsa.147540.2 vs. TAIR10
Match: AT3G42628.1 (AT3G42628.1 phosphoenolpyruvate carboxylase-related / PEP carboxylase-related)

HSP 1 Score: 79.0 bits (193), Expect = 1.4e-14
Identity = 39/44 (88.64%), Postives = 42/44 (95.45%), Query Frame = 1

Query: 3  AVKNLEKMASIDAQLRLLAPSKVSEDDKLVEYDALLLDRFLDIL 47
          A +NLEKMASIDAQLRLLAP KVS+DDKL+EYDALLLDRFLDIL
Sbjct: 2  AARNLEKMASIDAQLRLLAPGKVSKDDKLIEYDALLLDRFLDIL 45

BLAST of Cucsa.147540.2 vs. NCBI nr
Match: gi|525507254|ref|NP_001267665.1| (phosphoenolpyruvate carboxylase 2-like [Cucumis sativus])

HSP 1 Score: 1472.2 bits (3810), Expect = 0.0e+00
Identity = 738/740 (99.73%), Postives = 739/740 (99.86%), Query Frame = 1

Query: 1   MAAVKNLEKMASIDAQLRLLAPSKVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQE 60
           MAAVKNLEKMASIDAQLRLLAPSKVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQE
Sbjct: 1   MAAVKNLEKMASIDAQLRLLAPSKVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQE 60

Query: 61  CYEFAAEYERKRDPEKLEELGNALTSLDPGDSIVVAKSFSHMLSLANLAEEVQIAYRRRI 120
           CYEFAAEYERKRDPEKLEELGNALTSLDPGDSIVVAKSFSHMLSLANLAEEVQIAYRRRI
Sbjct: 61  CYEFAAEYERKRDPEKLEELGNALTSLDPGDSIVVAKSFSHMLSLANLAEEVQIAYRRRI 120

Query: 121 KLKKGGFADEANATTESDIEETFKRLLQLNKSPQEVFDALKNQTVDLVFTAHPTQSVRRS 180
           KLKKGGFADEANATTESDIEETFKRLLQLNKSPQEVFDALKNQTVDLVFTAHPTQSVRRS
Sbjct: 121 KLKKGGFADEANATTESDIEETFKRLLQLNKSPQEVFDALKNQTVDLVFTAHPTQSVRRS 180

Query: 181 LLQKHGRIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGM 240
           LLQKHGRIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGM
Sbjct: 181 LLQKHGRIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGM 240

Query: 241 SYFHETIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTR 300
           SYFHETIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTR
Sbjct: 241 SYFHETIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTR 300

Query: 301 DVCLLARMMAANLYFSQIEDLMFELSMWRCSSELQTRAEELHRSSRKDAKHYIEFWKQIP 360
           DVCLLARMMAANLYFSQIEDLMFELSMWRCSSELQTRAEELHRSSRKDAKHYIEFWKQIP
Sbjct: 301 DVCLLARMMAANLYFSQIEDLMFELSMWRCSSELQTRAEELHRSSRKDAKHYIEFWKQIP 360

Query: 361 PNEPYRVILGDVRDKLYNTRERARHLLSSGMSEIPEDTTFTDVEQFLEPLELCYRSLCAC 420
           PNEPYRVILGDVRDKLYNTRERARHLLSSGMSEIPEDTTFTDVEQFLEPLELCYRSLCAC
Sbjct: 361 PNEPYRVILGDVRDKLYNTRERARHLLSSGMSEIPEDTTFTDVEQFLEPLELCYRSLCAC 420

Query: 421 GDRPIADGSLLDFLRQVSTFGLSLVRLDIRQESERHADVIDTITNYLGIGSYKEWSEEQK 480
           GDRPIADGSLLDFLRQVSTFGLSLVRLDIRQESERHADVIDTITNYLGIGSYKEWSEEQK
Sbjct: 421 GDRPIADGSLLDFLRQVSTFGLSLVRLDIRQESERHADVIDTITNYLGIGSYKEWSEEQK 480

Query: 481 QEWLLSELSGKRPLFGPDLPKTEETSDVLDTFSVIAELPSDNFGAYIISMATAPSDVLAV 540
           QEWLLSELSGKRPLFGPDLPKTEETSDVLDTFSVIAELPSDNFGAYIISMATAPSDVLAV
Sbjct: 481 QEWLLSELSGKRPLFGPDLPKTEETSDVLDTFSVIAELPSDNFGAYIISMATAPSDVLAV 540

Query: 541 ELLQRECHVQKPLRVVPLFEKLADLEAAPSAMARLFSIDWYRNRIDGKQEVMIGYSDSGK 600
           ELLQRECHVQKPLRVVPLFEKLADLEAAPSAMARLFSIDWYRNRIDGKQEVMIGYSDSGK
Sbjct: 541 ELLQRECHVQKPLRVVPLFEKLADLEAAPSAMARLFSIDWYRNRIDGKQEVMIGYSDSGK 600

Query: 601 DAGRLSAAWQLYKAQEELIQVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHG 660
           DAGRLSAAWQLYKAQEELIQVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHG
Sbjct: 601 DAGRLSAAWQLYKAQEELIQVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHG 660

Query: 661 SLRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMNPPVAPKPEWRALLDAMAVVATE 720
           SLRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMNPPVAPKPEWRALLDAMAVVATE
Sbjct: 661 SLRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMNPPVAPKPEWRALLDAMAVVATE 720

Query: 721 KYRSIVFQEPRFVEYFRLVS 741
           KYRSIVFQEPRFVEYFRL +
Sbjct: 721 KYRSIVFQEPRFVEYFRLAT 740

BLAST of Cucsa.147540.2 vs. NCBI nr
Match: gi|659110588|ref|XP_008455304.1| (PREDICTED: phosphoenolpyruvate carboxylase 2 [Cucumis melo])

HSP 1 Score: 1466.1 bits (3794), Expect = 0.0e+00
Identity = 734/740 (99.19%), Postives = 738/740 (99.73%), Query Frame = 1

Query: 1   MAAVKNLEKMASIDAQLRLLAPSKVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQE 60
           MAAVKNLEKMASIDAQLRLLAPSKVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQE
Sbjct: 1   MAAVKNLEKMASIDAQLRLLAPSKVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQE 60

Query: 61  CYEFAAEYERKRDPEKLEELGNALTSLDPGDSIVVAKSFSHMLSLANLAEEVQIAYRRRI 120
           CYEFAAEYERKRDP KLEELG+ALTSLDPGDSIVVAKSFSHMLSLANLAEEVQIAYRRRI
Sbjct: 61  CYEFAAEYERKRDPRKLEELGSALTSLDPGDSIVVAKSFSHMLSLANLAEEVQIAYRRRI 120

Query: 121 KLKKGGFADEANATTESDIEETFKRLLQLNKSPQEVFDALKNQTVDLVFTAHPTQSVRRS 180
           KLKKGGFADEANATTESDIEETFKRLLQLNKSPQEVFDALKNQTVDLVFTAHPTQSVRRS
Sbjct: 121 KLKKGGFADEANATTESDIEETFKRLLQLNKSPQEVFDALKNQTVDLVFTAHPTQSVRRS 180

Query: 181 LLQKHGRIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGM 240
           LLQKHGRIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGM
Sbjct: 181 LLQKHGRIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGM 240

Query: 241 SYFHETIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTR 300
           SYFHETIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTR
Sbjct: 241 SYFHETIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTR 300

Query: 301 DVCLLARMMAANLYFSQIEDLMFELSMWRCSSELQTRAEELHRSSRKDAKHYIEFWKQIP 360
           DVCLLARMMAANLYFSQIEDLMFELSMWRCSSELQTRAEELHRSSRKDAKHYIEFWKQIP
Sbjct: 301 DVCLLARMMAANLYFSQIEDLMFELSMWRCSSELQTRAEELHRSSRKDAKHYIEFWKQIP 360

Query: 361 PNEPYRVILGDVRDKLYNTRERARHLLSSGMSEIPEDTTFTDVEQFLEPLELCYRSLCAC 420
           PNEPYRVILGDVRDKLYNTRERARHLLSSGMSEIPEDTTFTDVEQFLEPLELCYRSLCAC
Sbjct: 361 PNEPYRVILGDVRDKLYNTRERARHLLSSGMSEIPEDTTFTDVEQFLEPLELCYRSLCAC 420

Query: 421 GDRPIADGSLLDFLRQVSTFGLSLVRLDIRQESERHADVIDTITNYLGIGSYKEWSEEQK 480
           GDRPIADGSLLDFLRQVSTFGLSLVRLDIRQESERHADVIDTITNYLGIGSYKEWSEEQK
Sbjct: 421 GDRPIADGSLLDFLRQVSTFGLSLVRLDIRQESERHADVIDTITNYLGIGSYKEWSEEQK 480

Query: 481 QEWLLSELSGKRPLFGPDLPKTEETSDVLDTFSVIAELPSDNFGAYIISMATAPSDVLAV 540
           QEWLLSELSGKRPLFGPDLPKTEET+DVLDTF+VIAELPSDNFGAYIISMATAPSDVLAV
Sbjct: 481 QEWLLSELSGKRPLFGPDLPKTEETADVLDTFNVIAELPSDNFGAYIISMATAPSDVLAV 540

Query: 541 ELLQRECHVQKPLRVVPLFEKLADLEAAPSAMARLFSIDWYRNRIDGKQEVMIGYSDSGK 600
           ELLQRECHVQKPLRVVPLFEKLADLEAAPSAMARLFSIDWYRNRIDGKQEVMIGYSDSGK
Sbjct: 541 ELLQRECHVQKPLRVVPLFEKLADLEAAPSAMARLFSIDWYRNRIDGKQEVMIGYSDSGK 600

Query: 601 DAGRLSAAWQLYKAQEELIQVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHG 660
           DAGRLSAAWQLYKAQEELIQVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHG
Sbjct: 601 DAGRLSAAWQLYKAQEELIQVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHG 660

Query: 661 SLRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMNPPVAPKPEWRALLDAMAVVATE 720
           SLRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMNPPVAPKPEWRALLDAMAVVATE
Sbjct: 661 SLRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMNPPVAPKPEWRALLDAMAVVATE 720

Query: 721 KYRSIVFQEPRFVEYFRLVS 741
           KYRSIVFQEPRFVEYFRL +
Sbjct: 721 KYRSIVFQEPRFVEYFRLAT 740

BLAST of Cucsa.147540.2 vs. NCBI nr
Match: gi|470134710|ref|XP_004303182.1| (PREDICTED: phosphoenolpyruvate carboxylase 2 [Fragaria vesca subsp. vesca])

HSP 1 Score: 1356.3 bits (3509), Expect = 0.0e+00
Identity = 671/739 (90.80%), Postives = 708/739 (95.81%), Query Frame = 1

Query: 3   AVKNLEKMASIDAQLRLLAPSKVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQECY 62
           A +NLEKMASIDAQLRLLAP KVSEDDKL+EYDALLLDRFLDILQDLHGEDLRETVQ+CY
Sbjct: 2   ASRNLEKMASIDAQLRLLAPGKVSEDDKLIEYDALLLDRFLDILQDLHGEDLRETVQDCY 61

Query: 63  EFAAEYERKRDPEKLEELGNALTSLDPGDSIVVAKSFSHMLSLANLAEEVQIAYRRRIKL 122
           E +AEYE K+DP+KLEELGN  TSLDPGDSIVVAKSFSHML+LANLAEEVQIAYRRRIKL
Sbjct: 62  ELSAEYEGKQDPKKLEELGNVFTSLDPGDSIVVAKSFSHMLNLANLAEEVQIAYRRRIKL 121

Query: 123 KKGGFADEANATTESDIEETFKRLL-QLNKSPQEVFDALKNQTVDLVFTAHPTQSVRRSL 182
           KKG F DEANATTESDIEETFK+L+ QL KSPQEVFD LKNQT+DLV TAHPTQSVRRSL
Sbjct: 122 KKGDFVDEANATTESDIEETFKKLVGQLKKSPQEVFDELKNQTIDLVLTAHPTQSVRRSL 181

Query: 183 LQKHGRIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMS 242
           LQKH R+RNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMS
Sbjct: 182 LQKHARVRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMS 241

Query: 243 YFHETIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRD 302
           YFHETIWKGVPKFLRRVDTALKNIGI+ERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRD
Sbjct: 242 YFHETIWKGVPKFLRRVDTALKNIGINERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRD 301

Query: 303 VCLLARMMAANLYFSQIEDLMFELSMWRCSSELQTRAEELHRSSRKDAKHYIEFWKQIPP 362
           VCLLARMMAANLYFSQIEDLMFELSMWRC+ EL  RA ELHRSSRKDAKHYIEFWKQIPP
Sbjct: 302 VCLLARMMAANLYFSQIEDLMFELSMWRCNEELLARAHELHRSSRKDAKHYIEFWKQIPP 361

Query: 363 NEPYRVILGDVRDKLYNTRERARHLLSSGMSEIPEDTTFTDVEQFLEPLELCYRSLCACG 422
           NEPYRVILG VRDKLYNTRERAR LLSS  S+IPEDTTFT VEQFLEPLELCYRSLC+CG
Sbjct: 362 NEPYRVILGGVRDKLYNTRERARQLLSSLTSDIPEDTTFTSVEQFLEPLELCYRSLCSCG 421

Query: 423 DRPIADGSLLDFLRQVSTFGLSLVRLDIRQESERHADVIDTITNYLGIGSYKEWSEEQKQ 482
           D+ IADGSLLDFLRQVSTFGLSLVRLDIRQES+RH DVIDTIT +LG+GSY+EW E+Q+Q
Sbjct: 422 DKAIADGSLLDFLRQVSTFGLSLVRLDIRQESDRHTDVIDTITKHLGLGSYREWPEKQRQ 481

Query: 483 EWLLSELSGKRPLFGPDLPKTEETSDVLDTFSVIAELPSDNFGAYIISMATAPSDVLAVE 542
           EWLLSELSGKRPLFG D+PKTEE SDVL+TF VI+ELPSDNFGAYIISMATAPSDVLAVE
Sbjct: 482 EWLLSELSGKRPLFGADIPKTEEVSDVLETFKVISELPSDNFGAYIISMATAPSDVLAVE 541

Query: 543 LLQRECHVQKPLRVVPLFEKLADLEAAPSAMARLFSIDWYRNRIDGKQEVMIGYSDSGKD 602
           LLQRECHV+KPLRVVPLFEKLADLEAAP+A+ARLFSIDWY+NRI+GKQEVMIGYSDSGKD
Sbjct: 542 LLQRECHVKKPLRVVPLFEKLADLEAAPAAVARLFSIDWYKNRINGKQEVMIGYSDSGKD 601

Query: 603 AGRLSAAWQLYKAQEELIQVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHGS 662
           AGRLSAAWQLYKAQEEL++VAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPP+TIHGS
Sbjct: 602 AGRLSAAWQLYKAQEELVKVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPETIHGS 661

Query: 663 LRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMNPPVAPKPEWRALLDAMAVVATEK 722
           LRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMNPPV+PKPEWRAL+D MAVVATE+
Sbjct: 662 LRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMNPPVSPKPEWRALMDEMAVVATEE 721

Query: 723 YRSIVFQEPRFVEYFRLVS 741
           YRSIVFQEPRFVEYFRL +
Sbjct: 722 YRSIVFQEPRFVEYFRLAT 740

BLAST of Cucsa.147540.2 vs. NCBI nr
Match: gi|1009161962|ref|XP_015899177.1| (PREDICTED: phosphoenolpyruvate carboxylase, housekeeping isozyme [Ziziphus jujuba])

HSP 1 Score: 1349.0 bits (3490), Expect = 0.0e+00
Identity = 667/739 (90.26%), Postives = 706/739 (95.53%), Query Frame = 1

Query: 3   AVKNLEKMASIDAQLRLLAPSKVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQECY 62
           A +NLEK+ASIDAQLRLL P+KVSEDDKLVEYDALLLDRFLDILQDLHGEDL+ETVQECY
Sbjct: 2   ANRNLEKLASIDAQLRLLVPAKVSEDDKLVEYDALLLDRFLDILQDLHGEDLKETVQECY 61

Query: 63  EFAAEYERKRDPEKLEELGNALTSLDPGDSIVVAKSFSHMLSLANLAEEVQIAYRRRIKL 122
           E +AEYE K DP+KLEELGN LTSLDPGDSIVVAKSFSHML+LANLAEEVQIAYRRR KL
Sbjct: 62  ELSAEYEGKHDPQKLEELGNVLTSLDPGDSIVVAKSFSHMLNLANLAEEVQIAYRRRNKL 121

Query: 123 KKGGFADEANATTESDIEETFKRLL-QLNKSPQEVFDALKNQTVDLVFTAHPTQSVRRSL 182
           KKG FADE +ATTESDIEET KRL+ QL KSPQE+FDALKNQTVDLV TAHPTQSVRRSL
Sbjct: 122 KKGDFADENSATTESDIEETLKRLVGQLKKSPQEIFDALKNQTVDLVLTAHPTQSVRRSL 181

Query: 183 LQKHGRIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMS 242
           LQKHGRIRNCL QLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMS
Sbjct: 182 LQKHGRIRNCLAQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMS 241

Query: 243 YFHETIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRD 302
           YFHETIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRD
Sbjct: 242 YFHETIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRD 301

Query: 303 VCLLARMMAANLYFSQIEDLMFELSMWRCSSELQTRAEELHRSSRKDAKHYIEFWKQIPP 362
           VCLLARMMAANLY+SQIEDLMFELSMWRC+ EL+ RA+ LHR S+KDAKHYIEFWKQIPP
Sbjct: 302 VCLLARMMAANLYYSQIEDLMFELSMWRCNDELRVRADVLHRFSKKDAKHYIEFWKQIPP 361

Query: 363 NEPYRVILGDVRDKLYNTRERARHLLSSGMSEIPEDTTFTDVEQFLEPLELCYRSLCACG 422
           +EPYRVILGDVRDKLY TRER+RHLL++G S+IPED TFT+VEQFLEPLELCYRSLC+CG
Sbjct: 362 SEPYRVILGDVRDKLYQTRERSRHLLANGYSDIPEDATFTNVEQFLEPLELCYRSLCSCG 421

Query: 423 DRPIADGSLLDFLRQVSTFGLSLVRLDIRQESERHADVIDTITNYLGIGSYKEWSEEQKQ 482
           DR IADGSLLDFLRQVSTFGLSLVRLDIRQES+RH DV+D IT +L IGSY+EWSEE++Q
Sbjct: 422 DRAIADGSLLDFLRQVSTFGLSLVRLDIRQESDRHTDVLDAITKHLEIGSYREWSEERRQ 481

Query: 483 EWLLSELSGKRPLFGPDLPKTEETSDVLDTFSVIAELPSDNFGAYIISMATAPSDVLAVE 542
           EWLL+ELSGKRPLFGPDLPKTEE +DVLDTF VIAELPSDNFGAYIISMATAPSDVLAVE
Sbjct: 482 EWLLAELSGKRPLFGPDLPKTEEIADVLDTFHVIAELPSDNFGAYIISMATAPSDVLAVE 541

Query: 543 LLQRECHVQKPLRVVPLFEKLADLEAAPSAMARLFSIDWYRNRIDGKQEVMIGYSDSGKD 602
           LLQRECHV+KPLRVVPLFEKLADLEAAP+A+ RLFS+DWYRNRIDGKQEVMIGYSDSGKD
Sbjct: 542 LLQRECHVKKPLRVVPLFEKLADLEAAPAALGRLFSVDWYRNRIDGKQEVMIGYSDSGKD 601

Query: 603 AGRLSAAWQLYKAQEELIQVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHGS 662
           AGRLSAAWQLYKAQEELI+VAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHGS
Sbjct: 602 AGRLSAAWQLYKAQEELIKVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHGS 661

Query: 663 LRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMNPPVAPKPEWRALLDAMAVVATEK 722
           LRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGM PPV+PKPEWRALLD M VVATE+
Sbjct: 662 LRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMRPPVSPKPEWRALLDEMTVVATEE 721

Query: 723 YRSIVFQEPRFVEYFRLVS 741
           YRSIVF+EPRFVEYFRL +
Sbjct: 722 YRSIVFKEPRFVEYFRLAT 740

BLAST of Cucsa.147540.2 vs. NCBI nr
Match: gi|802610033|ref|XP_012074173.1| (PREDICTED: phosphoenolpyruvate carboxylase 2 [Jatropha curcas])

HSP 1 Score: 1348.6 bits (3489), Expect = 0.0e+00
Identity = 664/739 (89.85%), Postives = 708/739 (95.81%), Query Frame = 1

Query: 3   AVKNLEKMASIDAQLRLLAPSKVSEDDKLVEYDALLLDRFLDILQDLHGEDLRETVQECY 62
           A KNLEKM SIDAQLRLLAP KVSEDDKLVEYDALLLDRFLDILQDLHGED+RETVQECY
Sbjct: 2   AGKNLEKMVSIDAQLRLLAPRKVSEDDKLVEYDALLLDRFLDILQDLHGEDIRETVQECY 61

Query: 63  EFAAEYERKRDPEKLEELGNALTSLDPGDSIVVAKSFSHMLSLANLAEEVQIAYRRRIKL 122
           E +AEYERK DP+KLEELG  LTSLDPGDSIVV KSFSHML+LANLAEEVQIAYRRR K 
Sbjct: 62  EISAEYERKHDPQKLEELGKVLTSLDPGDSIVVTKSFSHMLNLANLAEEVQIAYRRRTKS 121

Query: 123 KKGGFADEANATTESDIEETFKRLL-QLNKSPQEVFDALKNQTVDLVFTAHPTQSVRRSL 182
           KKG FADE +ATTESD+EETFK+L+ QL KSP+EVFDALKNQTVDLV TAHPTQSVRRSL
Sbjct: 122 KKGDFADENSATTESDMEETFKKLVVQLKKSPEEVFDALKNQTVDLVLTAHPTQSVRRSL 181

Query: 183 LQKHGRIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMS 242
           LQKH RIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMS
Sbjct: 182 LQKHARIRNCLTQLYAKDITPDDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMS 241

Query: 243 YFHETIWKGVPKFLRRVDTALKNIGIDERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRD 302
           YFHETIWKGVPKFLRRVDTALKNIGI+ER+PYNAPLIQFSSWMGGDRDGNPRVTPEVTRD
Sbjct: 242 YFHETIWKGVPKFLRRVDTALKNIGINERLPYNAPLIQFSSWMGGDRDGNPRVTPEVTRD 301

Query: 303 VCLLARMMAANLYFSQIEDLMFELSMWRCSSELQTRAEELHRSSRKDAKHYIEFWKQIPP 362
           VCLLARMMAANLYFSQIEDLMFELSMWRC+ EL+ RA+ LHRSSRKDAKHYIEFWKQIPP
Sbjct: 302 VCLLARMMAANLYFSQIEDLMFELSMWRCNEELRFRADALHRSSRKDAKHYIEFWKQIPP 361

Query: 363 NEPYRVILGDVRDKLYNTRERARHLLSSGMSEIPEDTTFTDVEQFLEPLELCYRSLCACG 422
           NEPYRVILGDVRDKLYNTRER+RHLL++G+S+IPE+ TFT+VEQFLEPLELCYRSLCACG
Sbjct: 362 NEPYRVILGDVRDKLYNTRERSRHLLTNGISDIPEEATFTNVEQFLEPLELCYRSLCACG 421

Query: 423 DRPIADGSLLDFLRQVSTFGLSLVRLDIRQESERHADVIDTITNYLGIGSYKEWSEEQKQ 482
           DRPIADGSLLDFLRQVSTFGLSLVRLDIRQES+RH DV+D IT +LGIGSY+EWSEE++Q
Sbjct: 422 DRPIADGSLLDFLRQVSTFGLSLVRLDIRQESDRHTDVLDAITKHLGIGSYREWSEERRQ 481

Query: 483 EWLLSELSGKRPLFGPDLPKTEETSDVLDTFSVIAELPSDNFGAYIISMATAPSDVLAVE 542
           EWLLSEL GKRPLFGPDLPKTEE +DVLDTF VIAELP DNFGAYIISMATAPSDVLAVE
Sbjct: 482 EWLLSELRGKRPLFGPDLPKTEEIADVLDTFHVIAELPPDNFGAYIISMATAPSDVLAVE 541

Query: 543 LLQRECHVQKPLRVVPLFEKLADLEAAPSAMARLFSIDWYRNRIDGKQEVMIGYSDSGKD 602
           LLQREC V++PLRVVPLFEKLADLEAAP+A++RLFSIDWYRNRI+GKQEVMIGYSDSGKD
Sbjct: 542 LLQRECRVKQPLRVVPLFEKLADLEAAPAAVSRLFSIDWYRNRINGKQEVMIGYSDSGKD 601

Query: 603 AGRLSAAWQLYKAQEELIQVAKQYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHGS 662
           AGRLSAAWQLYKAQEEL++VAKQYGVKL MFHGRGGTVGRGGGPTHLAILSQPPDTIHGS
Sbjct: 602 AGRLSAAWQLYKAQEELVKVAKQYGVKLVMFHGRGGTVGRGGGPTHLAILSQPPDTIHGS 661

Query: 663 LRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMNPPVAPKPEWRALLDAMAVVATEK 722
           LRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGM+PP++PKPEWRALLD MA++AT++
Sbjct: 662 LRVTVQGEVIEQSFGEEHLCFRTLQRFTAATLEHGMHPPISPKPEWRALLDEMAIIATKE 721

Query: 723 YRSIVFQEPRFVEYFRLVS 741
           YRSIVFQEPRFVEYFRL +
Sbjct: 722 YRSIVFQEPRFVEYFRLAT 740