Cucsa.143160.1 (mRNA) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.143160.1
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	V-type proton ATPase subunit F
Location	scaffold01079 : 1458882 .. 1462162 (+)
Sequence length	1026

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGACCTGGTTGAGCGTGCGAGAAACAAAACACAACTATAATTCTCCCTTCTTCTCCTTCGGGAAGTCCAGCGCCGCTTCCAGCTTCGCCTCTTCACTCCGCCGACCGCCCTCACCAACACGCCTCCTCCCTAGCCGTTACCGGCAACCAGATCTGTGCCGCTCGCCGTCACTATCAAAATCCTCCGATCACAATTCCGCCTTCTGGTATTTCTTCTAACTCCATTCCTTCTTCCTCATTTCTTCGATTTTTGCCCGATCTTCGTTCCTAGCTTCACCCCTCTTACTCCTGATCTTCACCCACAATCCTTGATTTTCATCCACGATTACACCTACAATCTTGGAGTTTCAGCTACCATTACTCCCATTTATTAACCTAAAATACATGGTTCGATTTCTGAGCTGTAAATTTTCGATCTCCCGATTTTTAGTTTCATTTCCTAGTTTATTTTTAGTTATTTGTTTCTGTTCATCTCGCTATGAATTGGAGCTTCAACCCATCTGGGATCACTTTCCCCCGCCTCTGCTCTCGCTTAATTGGATAGGGATTTTCAGTTCAAACTCTTCATTTTGTAATTCCAATATTTTCCTTGTTTTGTTAATATAATTATTTATAATTTATTTTTGTAGTTACATCTCATAAATGGCTGGCAAAGCTCAAATTCCAACCAAGAATTCAGCGCTTATTTCTATGATTGCTGATGAGGTACGCCCAATATATATCCCTTATTGCAACAGCTTTTTCTCGGTACCTTAAACTATTAAATTGAATTGCGGTTGAGTAGGAGTTCATACTACGTATTCACCCTTTGATCTTATTATGTGATTTTGCATTTTGGTATTTGGTTTGATTTGTATAGATAAAGCATCCACTTTTGTGATCCTATTGTTATGCGATATGTGTTTTAAGTACCCCGTAAACCTGTGTTTGTAAGGTTGTTGTCAAAGCGAATCTGCTTGTCAATTACGTTCTCATAAGTACTTTAATCTTCAAAGGTGATCCCTTTATTGTTAGTTTTTCTCTGAAATTCTCAACTTTTCTCAATAAACAATCATTACACATTTAGTTCCTCCAGTGATTTTAGGTTCATTCTCTTCATTTTGGCATAATTGAGCATCTGGATAATGATAATGATAGGGTCCAACTCCTTCTGTTGTGTTGTGCATTATGAACTATGCATGGATGTCAGTTCAGTTGTATGTTTAAACTAAGTAACCTTCATAATAGGATACGGTGGTTGGATTTCTTCTTGCTGGAGTTGGTAATGTCGACCTGCGAAGAAAAACTAATTACCTTATAGTGGACTCAAGTAAGTATATCCCTTTGAACCAAATTGCTTGTCACTGTGGATTTCTATTTCACTTGTAATTCTGATATACTATAATCTGTTTCATTAAGCTCACACTACGACTGTGTGTATAAATCCAGCAAAACTCCCCATTGGTGAAAATCATTGTTACTTAATAATTAAGATGGTCTGTTTTGAGGTTTTCATAAAAAATTGTCCAGTGATACAAATGAAATTCTGAATCAGCTTTCAAAAATGTTTCTCTATTTTTAATAAGGAAGAATTTCATTAATGACATGAATTTACAAAAGAGGGGCAAAGCCCCCTATCTAAGGAGACATAAAGTCTCTCAATTTGGAAAGAAGAAAATTTTAGCTATTAGTGGTGAAAGAGTGGAGAGCGTTTAGACGATGAAACAACCAAAAGAACTATTGCTTAAAAAATTTGTGCAATAAGAGATTCTTATCTTGAAAAATCTGACGGTTCCTTCCTTAGCAAATAGTCCAATAGAAAGCTTCCGGTGACATTCATCTGTAGGAGTGTTTTCTGCATTTTGAATGGGCGCCTCCCAAGTGTAAGAGAGGGTAAGAGTAGAGTAGGGTCATTTGGAAAAGCAGTGCACCATCTGAAAACTTTCAGAAAAATAACCCAAAATAAGCATGTGGCCTCTCATTCAAGCTATGTTGAGTAATCTCCCTACATATTATCTCTCCCTCTTCAAACTACCAAGTAAAGTAAGCTCACTTGATTGTAAAGCACTTTTTGTGGAAGGGGTAAGAAGAAGGGTCTGATCTACACCACCATCGTTGGCTGGGATAAAATTACTACTCCTTTCATCCATGGTGCTTTTAACTGGAATCTAGCTTATATTATACAAAATCCCCAATGAAAGTTCGGCAAAAAACATATGGTATCCTTGTCTTGATCCACAGAGGGGCACAATAAAAAATCTGCAGTAGTAAATTGATCTTCTTGTAAGGATAGCAGTTTTTTTATATTAATCTTTCTAATGAAGTGTATTTAAAATATTTTCATAGACTATTGTGCTCATGTCTTGGAGACATGTTTGGTTTGATGTCACATCGTCAATAGATCAATAGTAGCTACTAATGATCCTAATGTTATGATTGAACCATAAAGATTGATATTTGATAGGAATCTGTTCGTTTTATTCAGCTGTTTTGTGCGATTACATCTTTTTTTtGTCTCAAATGTTTGAACTTTTAATGTTTCAGAGACAACTGTTAAACAAATTGAAGATGCTTTCAAAGAGTTCACTACAAGGGAGGATATTGCAATAGTGATGATTAGTCAATATGTAAGTTGTTTTTTGAAATCCCTTTCTTTCTAATGCAAGTGACATATGACTTGTTTTATTATTATTATTCTTAATACTTATTTTTTTGAGTTTTAGGTTGCAAACATGATCAGGTTTCTGGTAGATAGCTATAACAAGCCAATTCCTGCAATATTGGAGATTCCTTCCAAGGATCATCCATATGATCCTGCTCATGATTCAGTTCTTTCACGAGTAAAGAACCTGTTTTCTACCGAATCGGTGGCATCCGGGAGGTATTAAAATGATATTGTAACTTTTGTCCCAACTCTTTATGAAGTCATCCACCTTTATTCAAACATGCTGCATTTTGCGTTCCCGCTTTCTGCCTTGAATTTTATTTTGCAATGATTTTATTCATATCAATCACTCGAATAAAACTCGGAAAATAGATGGCGTCAGAGGAGCTCGTGGTGTCCAAGCACCTTAGAATGCCACTTTTTTTttttTtttACTGTACCTTAATTCCTATATTTACAGCAACTTGAGACATTACATCTACATCTATCTTGTTCTCTTGGTGCATGTATGCCAAAAACCAAATACTGTTGTAATTACTCTTCTATTTGAGGCTACTCTTGGAGATTGAAGAACTACTTGTTCTTATTGCCTTCTTGAATCTTTTGTTAGCTTTCAATCTACGTTATTTAGTGGAAA

mRNA sequence

GTGACCTGGTTGAGCGTGCGAGAAACAAAACACAACTATAATTCTCCCTTCTTCTCCTTCGGGAAGTCCAGCGCCGCTTCCAGCTTCGCCTCTTCACTCCGCCGACCGCCCTCACCAACACGCCTCCTCCCTAGCCGTTACCGGCAACCAGATCTGTGCCGCTCGCCGTCACTATCAAAATCCTCCGATCACAATTCCGCCTTCTGTTACATCTCATAAATGGCTGGCAAAGCTCAAATTCCAACCAAGAATTCAGCGCTTATTTCTATGATTGCTGATGAGGATACGGTGGTTGGATTTCTTCTTGCTGGAGTTGGTAATGTCGACCTGCGAAGAAAAACTAATTACCTTATAGTGGACTCAAAGACAACTGTTAAACAAATTGAAGATGCTTTCAAAGAGTTCACTACAAGGGAGGATATTGCAATAGTGATGATTAGTCAATATGTTGCAAACATGATCAGGTTTCTGGTAGATAGCTATAACAAGCCAATTCCTGCAATATTGGAGATTCCTTCCAAGGATCATCCATATGATCCTGCTCATGATTCAGTTCTTTCACGAGTAAAGAACCTGTTTTCTACCGAATCGGTGGCATCCGGGAGGTATTAAAATGATATTGTAACTTTTGTCCCAACTCTTTATGAAGTCATCCACCTTTATTCAAACATGCTGCATTTTGCGTTCCCGCTTTCTGCCTTGAATTTTATTTTGCAATGATTTTATTCATATCAATCACTCGAATAAAACTCGGAAAATAGATGGCGTCAGAGGAGCTCGTGGTGTCCAAGCACCTTAGAATGCCACTTTTTTTTTTTTTTTACTGTACCTTAATTCCTATATTTACAGCAACTTGAGACATTACATCTACATCTATCTTGTTCTCTTGGTGCATGTATGCCAAAAACCAAATACTGTTGTAATTACTCTTCTATTTGAGGCTACTCTTGGAGATTGAAGAACTACTTGTTCTTATTGCCTTCTTGAATCTTTTGTTAGCTTTCAATCTACGTTATTTAGTGGAAA

Coding sequence (CDS)

ATGGCTGGCAAAGCTCAAATTCCAACCAAGAATTCAGCGCTTATTTCTATGATTGCTGATGAGGATACGGTGGTTGGATTTCTTCTTGCTGGAGTTGGTAATGTCGACCTGCGAAGAAAAACTAATTACCTTATAGTGGACTCAAAGACAACTGTTAAACAAATTGAAGATGCTTTCAAAGAGTTCACTACAAGGGAGGATATTGCAATAGTGATGATTAGTCAATATGTTGCAAACATGATCAGGTTTCTGGTAGATAGCTATAACAAGCCAATTCCTGCAATATTGGAGATTCCTTCCAAGGATCATCCATATGATCCTGCTCATGATTCAGTTCTTTCACGAGTAAAGAACCTGTTTTCTACCGAATCGGTGGCATCCGGGAGGTATTAA

Protein sequence

MAGKAQIPTKNSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTTVKQIEDAFKEFTTREDIAIVMISQYVANMIRFLVDSYNKPIPAILEIPSKDHPYDPAHDSVLSRVKNLFSTESVASGRY*

Homology

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BLAST of Cucsa.143160.1 vs. Swiss-Prot
Match: VATF_ARATH (V-type proton ATPase subunit F OS=Arabidopsis thaliana GN=VHA-F PE=2 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 4.2e-57
Identity = 107/126 (84.92%), Postives = 121/126 (96.03%), Query Frame = 1


Query: 1   MAGKAQIPTKNSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTTVKQIEDAFK 60
           MAG+A IP +NSALI+MIADEDTVVGFL+AGVGNVD+RRKTNYLIVDSKTTV+QIEDAFK
Sbjct: 1   MAGRATIPARNSALIAMIADEDTVVGFLMAGVGNVDIRRKTNYLIVDSKTTVRQIEDAFK 60

Query: 61  EFTTREDIAIVMISQYVANMIRFLVDSYNKPIPAILEIPSKDHPYDPAHDSVLSRVKNLF 120
           EF+ R+DIAI+++SQY+ANMIRFLVDSYNKP+PAILEIPSKDHPYDPAHDSVLSRVK LF
Sbjct: 61  EFSARDDIAIILLSQYIANMIRFLVDSYNKPVPAILEIPSKDHPYDPAHDSVLSRVKYLF 120

Query: 121 STESVA 127
           S ESV+
Sbjct: 121 SAESVS 126

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BLAST of Cucsa.143160.1 vs. Swiss-Prot
Match: VATF_DICDI (V-type proton ATPase subunit F OS=Dictyostelium discoideum GN=vatF PE=3 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 2.1e-32
Identity = 66/112 (58.93%), Postives = 88/112 (78.57%), Query Frame = 1


Query: 12  SALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTTVKQIEDAFKEFTTREDIAIV 71
           +AL+++I DED V GFLLAGVG  D ++  N+L+VDSKT+  +IE AFK FTTR DIAI+
Sbjct: 9   TALVAVIGDEDVVTGFLLAGVGQKDKKKNENFLVVDSKTSQAKIETAFKSFTTRNDIAII 68

Query: 72  MISQYVANMIRFLVDSYNKPIPAILEIPSKDHPYDPAHDSVLSRVKNLFSTE 124
           MI+Q VA+ IR+L+D Y++ IP ILEIPSKDHPYDP  DSV+ +VK +  ++
Sbjct: 69  MITQKVADEIRYLIDEYHQVIPTILEIPSKDHPYDPKKDSVMLKVKKMTGSD 120

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BLAST of Cucsa.143160.1 vs. Swiss-Prot
Match: VATF_CAEEL (Probable V-type proton ATPase subunit F OS=Caenorhabditis elegans GN=vha-9 PE=3 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 3.6e-32
Identity = 63/110 (57.27%), Postives = 90/110 (81.82%), Query Frame = 1


Query: 14  LISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTTVKQIEDAFKEFTTREDIAIVMI 73
           ++++I DEDTVVGFLL GVG ++  RK NYLIVD +TTV++IE+AF  F  R+DIAI++I
Sbjct: 9   ILAVIGDEDTVVGFLLGGVGELNKARKPNYLIVDKQTTVQEIEEAFNGFCARDDIAIILI 68

Query: 74  SQYVANMIRFLVDSYNKPIPAILEIPSKDHPYDPAHDSVLSRVKNLFSTE 124
           +Q++A MIR+ VD++ + IPA+LEIPSK+ PYDP+ DS+L+R + LF+ E
Sbjct: 69  NQHIAEMIRYAVDNHTQSIPAVLEIPSKEAPYDPSKDSILNRARGLFNPE 118

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BLAST of Cucsa.143160.1 vs. Swiss-Prot
Match: VATF_RAT (V-type proton ATPase subunit F OS=Rattus norvegicus GN=Atp6v1f PE=1 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 1.2e-30
Identity = 57/112 (50.89%), Postives = 85/112 (75.89%), Query Frame = 1


Query: 14  LISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTTVKQIEDAFKEFTTREDIAIVMI 73
           LI++I DEDTV GFLL G+G ++  R  N+L+V+  TT+ +IED F++F  R+DI I++I
Sbjct: 7   LIAVIGDEDTVTGFLLGGIGELNKNRHPNFLVVEKDTTINEIEDTFRQFLNRDDIGIILI 66

Query: 74  SQYVANMIRFLVDSYNKPIPAILEIPSKDHPYDPAHDSVLSRVKNLFSTESV 126
           +QY+A M+R  +D++ + IPA+LEIPSK+HPYD A DS+L R K +F+ E +
Sbjct: 67  NQYIAEMVRHALDAHQRSIPAVLEIPSKEHPYDAAKDSILRRAKGMFTAEDL 118

[Close]

BLAST of Cucsa.143160.1 vs. Swiss-Prot
Match: VATF_ANOGA (V-type proton ATPase subunit F OS=Anopheles gambiae GN=Vha14 PE=2 SV=2)

HSP 1 Score: 134.0 bits (336), Expect = 1.2e-30
Identity = 62/125 (49.60%), Postives = 85/125 (68.00%), Query Frame = 1


Query: 5   AQIPTKNSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTTVKQIEDAFKEFTT 64
           A +      LIS+I DEDT VGFLL GVG ++  R  N+++VD  T V +IED FK F  
Sbjct: 2   ALLSAMKGKLISVIGDEDTCVGFLLGGVGEINKNRHPNFMVVDKNTAVSEIEDCFKRFIK 61

Query: 65  REDIAIVMISQYVANMIRFLVDSYNKPIPAILEIPSKDHPYDPAHDSVLSRVKNLFSTES 124
           R+DI I++I+Q  A +IR ++DS+  P PA+LEIPSKDHPYD + DS+L R K +F+ E 
Sbjct: 62  RDDIDIILINQNYAELIRHVIDSHTAPTPAVLEIPSKDHPYDASKDSILRRAKGMFNPED 121

Query: 125 VASGR 130
           + + R
Sbjct: 122 MIANR 126

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BLAST of Cucsa.143160.1 vs. TrEMBL
Match: A0A0A0LX69_CUCSA (V-type proton ATPase subunit F OS=Cucumis sativus GN=Csa_1G611810 PE=3 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 6.5e-65
Identity = 130/130 (100.00%), Postives = 130/130 (100.00%), Query Frame = 1


Query: 1   MAGKAQIPTKNSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTTVKQIEDAFK 60
           MAGKAQIPTKNSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTTVKQIEDAFK
Sbjct: 1   MAGKAQIPTKNSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTTVKQIEDAFK 60

Query: 61  EFTTREDIAIVMISQYVANMIRFLVDSYNKPIPAILEIPSKDHPYDPAHDSVLSRVKNLF 120
           EFTTREDIAIVMISQYVANMIRFLVDSYNKPIPAILEIPSKDHPYDPAHDSVLSRVKNLF
Sbjct: 61  EFTTREDIAIVMISQYVANMIRFLVDSYNKPIPAILEIPSKDHPYDPAHDSVLSRVKNLF 120

Query: 121 STESVASGRY 131
           STESVASGRY
Sbjct: 121 STESVASGRY 130

[Close]

BLAST of Cucsa.143160.1 vs. TrEMBL
Match: V4T9F3_9ROSI (V-type proton ATPase subunit F OS=Citrus clementina GN=CICLE_v10002833mg PE=3 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 2.2e-60
Identity = 121/129 (93.80%), Postives = 126/129 (97.67%), Query Frame = 1


Query: 1   MAGKAQIPTKNSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTTVKQIEDAFK 60
           MAG+AQIPTK+SALI+MIADEDTV GFLLAGVGNVDLRRKTNYLIVDSKTTVK IEDAFK
Sbjct: 1   MAGRAQIPTKSSALIAMIADEDTVTGFLLAGVGNVDLRRKTNYLIVDSKTTVKAIEDAFK 60

Query: 61  EFTTREDIAIVMISQYVANMIRFLVDSYNKPIPAILEIPSKDHPYDPAHDSVLSRVKNLF 120
           EFTT+EDIAIV+ISQYVANMIRFLVDSYNKPIPAILEIPSKDHPYDPAHDSVLSRVKNLF
Sbjct: 61  EFTTKEDIAIVLISQYVANMIRFLVDSYNKPIPAILEIPSKDHPYDPAHDSVLSRVKNLF 120

Query: 121 STESVASGR 130
           S ESVASGR
Sbjct: 121 SAESVASGR 129

[Close]

BLAST of Cucsa.143160.1 vs. TrEMBL
Match: A0A067E4V9_CITSI (V-type proton ATPase subunit F OS=Citrus sinensis GN=CISIN_1g037034mg PE=3 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 2.2e-60
Identity = 121/129 (93.80%), Postives = 126/129 (97.67%), Query Frame = 1


Query: 1   MAGKAQIPTKNSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTTVKQIEDAFK 60
           MAG+AQIPTK+SALI+MIADEDTV GFLLAGVGNVDLRRKTNYLIVDSKTTVK IEDAFK
Sbjct: 1   MAGRAQIPTKSSALIAMIADEDTVTGFLLAGVGNVDLRRKTNYLIVDSKTTVKAIEDAFK 60

Query: 61  EFTTREDIAIVMISQYVANMIRFLVDSYNKPIPAILEIPSKDHPYDPAHDSVLSRVKNLF 120
           EFTT+EDIAIV+ISQYVANMIRFLVDSYNKPIPAILEIPSKDHPYDPAHDSVLSRVKNLF
Sbjct: 61  EFTTKEDIAIVLISQYVANMIRFLVDSYNKPIPAILEIPSKDHPYDPAHDSVLSRVKNLF 120

Query: 121 STESVASGR 130
           S ESVASGR
Sbjct: 121 SAESVASGR 129

[Close]

BLAST of Cucsa.143160.1 vs. TrEMBL
Match: A5B4N7_VITVI (V-type proton ATPase subunit F OS=Vitis vinifera GN=VIT_05s0062g00210 PE=3 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 8.3e-60
Identity = 118/129 (91.47%), Postives = 126/129 (97.67%), Query Frame = 1


Query: 1   MAGKAQIPTKNSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTTVKQIEDAFK 60
           MAG+AQIPTK+SALI+MIADEDT+ GFLLAGVGNVDLRRKTNYLIVDSKTTVKQIEDAFK
Sbjct: 1   MAGRAQIPTKSSALIAMIADEDTITGFLLAGVGNVDLRRKTNYLIVDSKTTVKQIEDAFK 60

Query: 61  EFTTREDIAIVMISQYVANMIRFLVDSYNKPIPAILEIPSKDHPYDPAHDSVLSRVKNLF 120
           EFTT+EDIAI++ISQYVANMIRFLVDSYNKP+PAILEIPSKDHPYDPAHDSVLSRVK LF
Sbjct: 61  EFTTKEDIAIILISQYVANMIRFLVDSYNKPVPAILEIPSKDHPYDPAHDSVLSRVKYLF 120

Query: 121 STESVASGR 130
           S ESVASGR
Sbjct: 121 SAESVASGR 129

[Close]

BLAST of Cucsa.143160.1 vs. TrEMBL
Match: C6SVI1_SOYBN (V-type proton ATPase subunit F OS=Glycine max GN=GLYMA_05G135300 PE=2 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 1.4e-59
Identity = 118/129 (91.47%), Postives = 125/129 (96.90%), Query Frame = 1


Query: 1   MAGKAQIPTKNSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTTVKQIEDAFK 60
           MA + QIPT NSALI+MIADEDTVVGFLLAGVGNVD+RRKTNYLIVDSKTTVKQIEDAFK
Sbjct: 1   MANRVQIPTNNSALIAMIADEDTVVGFLLAGVGNVDIRRKTNYLIVDSKTTVKQIEDAFK 60

Query: 61  EFTTREDIAIVMISQYVANMIRFLVDSYNKPIPAILEIPSKDHPYDPAHDSVLSRVKNLF 120
           EFTTRED+AIV+ISQYVANMIRFLVDSYNKP+PAILEIPSKDHPYDPAHDSVLSRVK LF
Sbjct: 61  EFTTREDVAIVLISQYVANMIRFLVDSYNKPVPAILEIPSKDHPYDPAHDSVLSRVKYLF 120

Query: 121 STESVASGR 130
           +TESVASGR
Sbjct: 121 NTESVASGR 129

[Close]

BLAST of Cucsa.143160.1 vs. TAIR10
Match: AT4G02620.1 (AT4G02620.1 vacuolar ATPase subunit F family protein)

HSP 1 Score: 221.9 bits (564), Expect = 2.4e-58
Identity = 107/126 (84.92%), Postives = 121/126 (96.03%), Query Frame = 1


Query: 1   MAGKAQIPTKNSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTTVKQIEDAFK 60
           MAG+A IP +NSALI+MIADEDTVVGFL+AGVGNVD+RRKTNYLIVDSKTTV+QIEDAFK
Sbjct: 1   MAGRATIPARNSALIAMIADEDTVVGFLMAGVGNVDIRRKTNYLIVDSKTTVRQIEDAFK 60

Query: 61  EFTTREDIAIVMISQYVANMIRFLVDSYNKPIPAILEIPSKDHPYDPAHDSVLSRVKNLF 120
           EF+ R+DIAI+++SQY+ANMIRFLVDSYNKP+PAILEIPSKDHPYDPAHDSVLSRVK LF
Sbjct: 61  EFSARDDIAIILLSQYIANMIRFLVDSYNKPVPAILEIPSKDHPYDPAHDSVLSRVKYLF 120

Query: 121 STESVA 127
           S ESV+
Sbjct: 121 SAESVS 126

[Close]

BLAST of Cucsa.143160.1 vs. NCBI nr
Match: gi|449466929|ref|XP_004151178.1| (PREDICTED: V-type proton ATPase subunit F [Cucumis sativus])

HSP 1 Score: 254.6 bits (649), Expect = 9.4e-65
Identity = 130/130 (100.00%), Postives = 130/130 (100.00%), Query Frame = 1


Query: 1   MAGKAQIPTKNSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTTVKQIEDAFK 60
           MAGKAQIPTKNSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTTVKQIEDAFK
Sbjct: 1   MAGKAQIPTKNSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTTVKQIEDAFK 60

Query: 61  EFTTREDIAIVMISQYVANMIRFLVDSYNKPIPAILEIPSKDHPYDPAHDSVLSRVKNLF 120
           EFTTREDIAIVMISQYVANMIRFLVDSYNKPIPAILEIPSKDHPYDPAHDSVLSRVKNLF
Sbjct: 61  EFTTREDIAIVMISQYVANMIRFLVDSYNKPIPAILEIPSKDHPYDPAHDSVLSRVKNLF 120

Query: 121 STESVASGRY 131
           STESVASGRY
Sbjct: 121 STESVASGRY 130

[Close]

BLAST of Cucsa.143160.1 vs. NCBI nr
Match: gi|1012187115|ref|XP_015969737.1| (PREDICTED: V-type proton ATPase subunit F-like [Arachis duranensis])

HSP 1 Score: 240.7 bits (613), Expect = 1.4e-60
Identity = 122/129 (94.57%), Postives = 126/129 (97.67%), Query Frame = 1


Query: 1   MAGKAQIPTKNSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTTVKQIEDAFK 60
           MA +AQIPTKNSALI+MIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTTVKQIEDAFK
Sbjct: 1   MANRAQIPTKNSALIAMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTTVKQIEDAFK 60

Query: 61  EFTTREDIAIVMISQYVANMIRFLVDSYNKPIPAILEIPSKDHPYDPAHDSVLSRVKNLF 120
           EFTTREDIAIV+ISQYVANMIRFLVDSYNKP+PAILEIPSKDHPYDPAHDSVLSRVK LF
Sbjct: 61  EFTTREDIAIVLISQYVANMIRFLVDSYNKPVPAILEIPSKDHPYDPAHDSVLSRVKYLF 120

Query: 121 STESVASGR 130
           S ESVASGR
Sbjct: 121 SAESVASGR 129

[Close]

BLAST of Cucsa.143160.1 vs. NCBI nr
Match: gi|1012103251|ref|XP_015957950.1| (PREDICTED: V-type proton ATPase subunit F [Arachis duranensis])

HSP 1 Score: 240.0 bits (611), Expect = 2.4e-60
Identity = 121/129 (93.80%), Postives = 126/129 (97.67%), Query Frame = 1


Query: 1   MAGKAQIPTKNSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTTVKQIEDAFK 60
           MA +AQIPTKNSALI+MIADEDTVVGFLLAGVGNVD+RRKTNYLIVDSKTTVKQIEDAFK
Sbjct: 1   MANRAQIPTKNSALIAMIADEDTVVGFLLAGVGNVDIRRKTNYLIVDSKTTVKQIEDAFK 60

Query: 61  EFTTREDIAIVMISQYVANMIRFLVDSYNKPIPAILEIPSKDHPYDPAHDSVLSRVKNLF 120
           EFTTREDIAIV+ISQYVANMIRFLVDSYNKP+PAILEIPSKDHPYDPAHDSVLSRVK LF
Sbjct: 61  EFTTREDIAIVLISQYVANMIRFLVDSYNKPVPAILEIPSKDHPYDPAHDSVLSRVKYLF 120

Query: 121 STESVASGR 130
           S ESVASGR
Sbjct: 121 SAESVASGR 129

[Close]

BLAST of Cucsa.143160.1 vs. NCBI nr
Match: gi|567880745|ref|XP_006432931.1| (hypothetical protein CICLE_v10002833mg [Citrus clementina])

HSP 1 Score: 239.6 bits (610), Expect = 3.1e-60
Identity = 121/129 (93.80%), Postives = 126/129 (97.67%), Query Frame = 1


Query: 1   MAGKAQIPTKNSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTTVKQIEDAFK 60
           MAG+AQIPTK+SALI+MIADEDTV GFLLAGVGNVDLRRKTNYLIVDSKTTVK IEDAFK
Sbjct: 1   MAGRAQIPTKSSALIAMIADEDTVTGFLLAGVGNVDLRRKTNYLIVDSKTTVKAIEDAFK 60

Query: 61  EFTTREDIAIVMISQYVANMIRFLVDSYNKPIPAILEIPSKDHPYDPAHDSVLSRVKNLF 120
           EFTT+EDIAIV+ISQYVANMIRFLVDSYNKPIPAILEIPSKDHPYDPAHDSVLSRVKNLF
Sbjct: 61  EFTTKEDIAIVLISQYVANMIRFLVDSYNKPIPAILEIPSKDHPYDPAHDSVLSRVKNLF 120

Query: 121 STESVASGR 130
           S ESVASGR
Sbjct: 121 SAESVASGR 129

[Close]

BLAST of Cucsa.143160.1 vs. NCBI nr
Match: gi|1021496452|ref|XP_016191262.1| (PREDICTED: V-type proton ATPase subunit F [Arachis ipaensis])

HSP 1 Score: 239.6 bits (610), Expect = 3.1e-60
Identity = 120/129 (93.02%), Postives = 126/129 (97.67%), Query Frame = 1


Query: 1   MAGKAQIPTKNSALISMIADEDTVVGFLLAGVGNVDLRRKTNYLIVDSKTTVKQIEDAFK 60
           MA +AQIPTKNSALI+MIADEDTVVGFLLAGVGN+D+RRKTNYLIVDSKTTVKQIEDAFK
Sbjct: 1   MANRAQIPTKNSALIAMIADEDTVVGFLLAGVGNIDIRRKTNYLIVDSKTTVKQIEDAFK 60

Query: 61  EFTTREDIAIVMISQYVANMIRFLVDSYNKPIPAILEIPSKDHPYDPAHDSVLSRVKNLF 120
           EFTTREDIAIV+ISQYVANMIRFLVDSYNKP+PAILEIPSKDHPYDPAHDSVLSRVK LF
Sbjct: 61  EFTTREDIAIVLISQYVANMIRFLVDSYNKPVPAILEIPSKDHPYDPAHDSVLSRVKYLF 120

Query: 121 STESVASGR 130
           S ESVASGR
Sbjct: 121 SAESVASGR 129

The following BLAST results are available for this feature:

BLAST of Cucsa.143160.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
VATF_ARATH	4.2e-57	84.92	V-type proton ATPase subunit F OS=Arabidopsis thaliana GN=VHA-F PE=2 SV=1	[more]
VATF_DICDI	2.1e-32	58.93	V-type proton ATPase subunit F OS=Dictyostelium discoideum GN=vatF PE=3 SV=1	[more]
VATF_CAEEL	3.6e-32	57.27	Probable V-type proton ATPase subunit F OS=Caenorhabditis elegans GN=vha-9 PE=3 ...	[more]
VATF_RAT	1.2e-30	50.89	V-type proton ATPase subunit F OS=Rattus norvegicus GN=Atp6v1f PE=1 SV=1	[more]
VATF_ANOGA	1.2e-30	49.60	V-type proton ATPase subunit F OS=Anopheles gambiae GN=Vha14 PE=2 SV=2	[more]

BLAST of Cucsa.143160.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LX69_CUCSA	6.5e-65	100.00	V-type proton ATPase subunit F OS=Cucumis sativus GN=Csa_1G611810 PE=3 SV=1	[more]
V4T9F3_9ROSI	2.2e-60	93.80	V-type proton ATPase subunit F OS=Citrus clementina GN=CICLE_v10002833mg PE=3 SV...	[more]
A0A067E4V9_CITSI	2.2e-60	93.80	V-type proton ATPase subunit F OS=Citrus sinensis GN=CISIN_1g037034mg PE=3 SV=1	[more]
A5B4N7_VITVI	8.3e-60	91.47	V-type proton ATPase subunit F OS=Vitis vinifera GN=VIT_05s0062g00210 PE=3 SV=1	[more]
C6SVI1_SOYBN	1.4e-59	91.47	V-type proton ATPase subunit F OS=Glycine max GN=GLYMA_05G135300 PE=2 SV=1	[more]

BLAST of Cucsa.143160.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G02620.1	2.4e-58	84.92	vacuolar ATPase subunit F family protein	[more]

BLAST of Cucsa.143160.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449466929\|ref\|XP_004151178.1\|	9.4e-65	100.00	PREDICTED: V-type proton ATPase subunit F [Cucumis sativus]	[more]
gi\|1012187115\|ref\|XP_015969737.1\|	1.4e-60	94.57	PREDICTED: V-type proton ATPase subunit F-like [Arachis duranensis]	[more]
gi\|1012103251\|ref\|XP_015957950.1\|	2.4e-60	93.80	PREDICTED: V-type proton ATPase subunit F [Arachis duranensis]	[more]
gi\|567880745\|ref\|XP_006432931.1\|	3.1e-60	93.80	hypothetical protein CICLE_v10002833mg [Citrus clementina]	[more]
gi\|1021496452\|ref\|XP_016191262.1\|	3.1e-60	93.02	PREDICTED: V-type proton ATPase subunit F [Arachis ipaensis]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR005772	ATPase_V1-cplx_fsu_euk
IPR008218	ATPase_V1-cplx_f_g_su

Vocabulary: **Cellular Component**
Term	Definition
GO:0033180	proton-transporting V-type ATPase, V1 domain

Vocabulary: **Molecular Function**
Term	Definition
GO:0046961	proton-transporting ATPase activity, rotational mechanism

Vocabulary: **Biological Process**
Term	Definition
GO:0015991	ATP hydrolysis coupled proton transport
GO:0034220	ion transmembrane transport

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015991	ATP hydrolysis coupled proton transport
biological_process	GO:0034220	ion transmembrane transport
biological_process	GO:0006119	oxidative phosphorylation
cellular_component	GO:0033180	proton-transporting V-type ATPase, V1 domain
cellular_component	GO:0005794	Golgi apparatus
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0046961	proton-transporting ATPase activity, rotational mechanism

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsa.143160	Cucsa.143160	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsa.143160.1	Cucsa.143160.1-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.143160.1.five_prime_UTR.1	Cucsa.143160.1.five_prime_UTR.1	five_prime_UTR
Cucsa.143160.1.five_prime_UTR.2	Cucsa.143160.1.five_prime_UTR.2	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.143160.1.CDS.1	Cucsa.143160.1.CDS.1	CDS
Cucsa.143160.1.CDS.2	Cucsa.143160.1.CDS.2	CDS
Cucsa.143160.1.CDS.3	Cucsa.143160.1.CDS.3	CDS
Cucsa.143160.1.CDS.4	Cucsa.143160.1.CDS.4	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.143160.1.three_prime_UTR.1	Cucsa.143160.1.three_prime_UTR.1	three_prime_UTR

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005772	ATPase, V1 complex, subunit F, eukaryotic	PIR	PIRSF015945	V-ATP_synth_F	coord: 3..123 score: 2.0
IPR005772	ATPase, V1 complex, subunit F, eukaryotic	PANTHER	PTHR13861	VACUOLAR ATP SYNTHASE SUBUNIT F	coord: 1..129 score: 7.8E
IPR005772	ATPase, V1 complex, subunit F, eukaryotic	TIGRFAMs	TIGR01101	TIGR01101	coord: 11..124 score: 1.6
IPR008218	ATPase, V1 complex, subunit F	GENE3D	G3DSA:3.40.50.10580		coord: 14..120 score: 4.0
IPR008218	ATPase, V1 complex, subunit F	PFAM	PF01990	ATP-synt_F	coord: 15..116 score: 1.8
IPR008218	ATPase, V1 complex, subunit F	unknown	SSF159468	AtpF-like	coord: 15..118 score: 4.97
None	No IPR available	PANTHER	PTHR13861:SF4	SUBFAMILY NOT NAMED	coord: 1..129 score: 7.8E