Cucsa.138430.1 (mRNA) Cucumber (Gy14) v1

BLAST of Cucsa.138430.1 vs. Swiss-Prot
Match: PGLR_PRUPE (Polygalacturonase OS=Prunus persica PE=2 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 4.8e-149
Identity = 255/386 (66.06%), Postives = 310/386 (80.31%), Query Frame = 1

Query: 3   FHKTLFFPFLFLT-LASASTFNVVDFGAKPNVQTDSSQAFEAAWASACRARTAVSIYVPK 62
           F  +L F F+  + +AS  T+NV   GAK + +TDS++AF +AWA AC +     IYVP 
Sbjct: 8   FSLSLIFVFMINSAIASPLTYNVASLGAKADGKTDSTKAFLSAWAKACASMNPGVIYVPA 67

Query: 63  GRFYVASLAFEGPCNNNDITIRIDGTLLAPSNYGVIANSGNWITFRRVDGVTLFGGVLDA 122
           G F++  + F GPC NN IT RI GTL+APS+Y VI N+ NWI F  V+GVT+ GG+LD 
Sbjct: 68  GTFFLRDVVFSGPCKNNAITFRIAGTLVAPSDYRVIGNAANWIFFHHVNGVTISGGILDG 127

Query: 123 QGSGLWACKNSKS-SCPPGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQGLK 182
           QG+ LWACK     SCP GAT+L FS+S NI+VSGL SLNSQMFHIVIN  QNV+ QG++
Sbjct: 128 QGTALWACKACHGESCPSGATTLGFSDSNNIVVSGLASLNSQMFHIVINDFQNVQMQGVR 187

Query: 183 VSAAGNSPNTDGIHVALSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGISIG 242
           VS +GNSPNTDGIHV +SS VTILNS I TGDDC+SIGPGTSNLWIE VACGPGHGISIG
Sbjct: 188 VSRSGNSPNTDGIHVQMSSGVTILNSKIATGDDCVSIGPGTSNLWIEGVACGPGHGISIG 247

Query: 243 SLGREVQEDGVENVTVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENPIII 302
           SLG+E +E GV+NVTVK+ TF+ TQNG+RIK+WG+PS GFAR+++FQ   MVNVENPI+I
Sbjct: 248 SLGKEQEEAGVQNVTVKTVTFSGTQNGLRIKSWGRPSTGFARNILFQHATMVNVENPIVI 307

Query: 303 DQNYCPDNKGCPGQDSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSC 362
           DQ+YCPDNKGCPGQ SGV+ISDVTY +IHGTSAT+VA+KFDCS K PC +I LEDVKL+ 
Sbjct: 308 DQHYCPDNKGCPGQVSGVQISDVTYEDIHGTSATEVAVKFDCSPKHPCREIKLEDVKLTY 367

Query: 363 KNEAAEASCSHAEGSAAGLVQPSSCL 387
           KN+AAE+SCSHA+G+  G+VQP+SCL
Sbjct: 368 KNQAAESSCSHADGTTEGVVQPTSCL 393

BLAST of Cucsa.138430.1 vs. Swiss-Prot
Match: PGLR6_ARATH (Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana GN=At2g43860 PE=2 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 1.3e-114
Identity = 208/369 (56.37%), Postives = 267/369 (72.36%), Query Frame = 1

Query: 20  STFNVVDFGAKPNVQTDSSQAFEAAWASACRARTAVSIYVPKGRFYVASLAFEG-PCNNN 79
           ST NV+ +GAKP+   DS++AF AAW  AC +    +I VPKGRF V +L F G  C   
Sbjct: 31  STLNVLSYGAKPDGSKDSTKAFLAAWDVACASANPTTIIVPKGRFLVGNLVFHGNECKQA 90

Query: 80  DITIRIDGTLLAPSNYGVIANSGNWITFRRVDGVTLFGGVLDAQGSGLWACKNSKS-SCP 139
            I+IRI G+++AP ++ +IA+S +WI F  V  V+++GG+LDAQG+ LW CKN+   +CP
Sbjct: 91  PISIRIAGSIVAPEDFRIIASSKHWIWFEDVTDVSIYGGILDAQGTSLWKCKNNGGHNCP 150

Query: 140 PGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQGLKVSAAGNSPNTDGIHVAL 199
            GA SL FS S NI +SGLTS+NSQ FHIVI+   NV   G+KVSA  NSPNTDGIHV  
Sbjct: 151 TGAKSLVFSGSNNIKISGLTSINSQKFHIVIDNSNNVNIDGVKVSADENSPNTDGIHVES 210

Query: 200 SSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGISIGSLGREVQEDGVENVTVK 259
           S +V I NS IGTGDDCISIGPG++N++I+ + CGPGHGISIGSLGR  +E GV+NVTV 
Sbjct: 211 SHSVHITNSRIGTGDDCISIGPGSTNVFIQTIRCGPGHGISIGSLGRAEEEQGVDNVTVS 270

Query: 260 SATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENPIIIDQNYCPDNKGCPGQDSG 319
           +  F  T NGVRIKTWGK SN FAR+++FQ I M  V+NPIIIDQ+YC  +K CP Q+SG
Sbjct: 271 NVDFMGTNNGVRIKTWGKDSNSFARNIVFQHINMKMVKNPIIIDQHYCL-HKPCPKQESG 330

Query: 320 VKISDVTYRNIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSCKNEAAEASCSHAEGSAA 379
           VK+S+V Y +IHGTS T+VA+  DCS + PC+ I ++DV L   +  A+ASC +A GSA 
Sbjct: 331 VKVSNVRYEDIHGTSNTEVAVLLDCSKEKPCTGIVMDDVNLVSVHRPAQASCDNANGSAN 390

Query: 380 GLVQPSSCL 387
            +V  + CL
Sbjct: 391 DVVPFTPCL 398

BLAST of Cucsa.138430.1 vs. Swiss-Prot
Match: PGLR2_PLAAC (Exopolygalacturonase (Fragment) OS=Platanus acerifolia GN=plaa2 PE=1 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 7.8e-83
Identity = 162/372 (43.55%), Postives = 229/372 (61.56%), Query Frame = 1

Query: 17  ASASTFNVVDFGAKPNVQTDSSQAFEAAWASACRARTAVSIYVPKGRFYVASLAFEGPCN 76
           +S S FNV D+GAK     D SQA   AW +AC ++   ++ +PKG + +  +A +GPC 
Sbjct: 5   SSGSVFNVNDYGAKG--AGDISQAVMKAWKAACASQGPSTVLIPKGNYNMGEVAMQGPCK 64

Query: 77  NNDITIRIDGTLLAPSNYGVIANSGNWITFRRVDGVTLFG-GVLDAQGSGLWACKN--SK 136
            + I  +IDG + AP++     + G W++F R+DG+T+ G G LD QG   WA  N    
Sbjct: 65  GSKIGFQIDGVVKAPADPSKFKSDG-WVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKN 124

Query: 137 SSCPPGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQGLKVSAAGNSPNTDGI 196
            +C   A +L F   K+ MV  +TSLNS+MFHI +  C+++  Q + V+A G S NTDGI
Sbjct: 125 PNCKHAAMNLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDGI 184

Query: 197 HVALSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGISIGSLGREVQEDGVEN 256
           HV +S  VTI N+ I TGDDCISIGPG+ N+ I  V CGPGHGISIGSLGR   E  V  
Sbjct: 185 HVGISKGVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVRG 244

Query: 257 VTVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENPIIIDQNYCPDNKGCPG 316
           +TVK  TF+ T NGVR+KTW     G A  + FQD+ M NV+NP+I+DQ YCP  +    
Sbjct: 245 ITVKGCTFSGTMNGVRVKTWPNSPPGAATDLTFQDLTMNNVQNPVILDQEYCPYGQCSRQ 304

Query: 317 QDSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSCKNEA--AEASCSH 376
             S +K+S++ + NI GTS  +VA+   CS   PCS++ + ++ LS +     A ++CS+
Sbjct: 305 APSRIKLSNINFNNIRGTSTGKVAVVIACSHGMPCSNMKIGEINLSYRGAGGPATSTCSN 364

Query: 377 AEGSAAGLVQPS 384
            + + +G   P+
Sbjct: 365 VKPTFSGKQVPA 373

BLAST of Cucsa.138430.1 vs. Swiss-Prot
Match: PGLR_ACTDE (Polygalacturonase OS=Actinidia deliciosa PE=2 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 8.9e-79
Identity = 162/373 (43.43%), Postives = 229/373 (61.39%), Query Frame = 1

Query: 16  LASASTFNVVDFGAKPNVQTDSSQAFEAAWASACRARTAVSIYVPKGRFYVASLAFEGPC 75
           L ++ T NV DFGAK + + D ++AFE AW +AC + ++  + VPK  + V  ++F GPC
Sbjct: 84  LDASKTVNVDDFGAKGDGR-DDTKAFEKAWKAACSSTSSAVLLVPKKNYLVRPISFSGPC 143

Query: 76  NNNDITIRIDGTLLAPSNYGVIANSG-NWITFRRVDGVTLFGG-VLDAQGSGLW--ACKN 135
            +  +T++I GT+ A  +       G +W+ F  V  + + GG  ++  G   W  +CK 
Sbjct: 144 KSG-LTMQIYGTIEASDDRSDYRKDGRHWLVFDSVQNLRVEGGGTINGNGKIWWQNSCKT 203

Query: 136 SKS-SCPPGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQGLKVSAAGNSPNT 195
           +K+  C    T+L F  SK+++V  L   N+Q  H+  + C NV+A  L V+A  NSPNT
Sbjct: 204 NKALPCKDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNLMVTAPENSPNT 263

Query: 196 DGIHVALSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGISIGSLGREVQEDG 255
           DGIHV  +  + I + +IGTGDDCISI  G+  + + ++ CGPGHGISIGSLG    E  
Sbjct: 264 DGIHVTGTQNIHISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGISIGSLGYGNSEAH 323

Query: 256 VENVTVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENPIIIDQNYCPDNKG 315
           V +V V  A    T NGVRIKTW +  +G A ++ FQ++ M NVENPIIIDQNYC  +K 
Sbjct: 324 VSDVVVNGAKLCGTTNGVRIKTW-QGGSGSASNIKFQNVEMHNVENPIIIDQNYCDQDKP 383

Query: 316 CPGQDSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSCK-NEAAEASC 375
           C  Q S V++ +V Y+NI GT A+ VA+ FDCS +FPC  I LEDV L  +   AA+A C
Sbjct: 384 CQEQSSAVQVKNVVYQNIKGTCASNVAITFDCSKRFPCQGIVLEDVDLEIEGGAAAKALC 443

Query: 376 SHAEGSAAGLVQP 383
           ++ E S  G+V P
Sbjct: 444 NNVELSETGVVSP 453

BLAST of Cucsa.138430.1 vs. Swiss-Prot
Match: PGLR3_MAIZE (Exopolygalacturonase OS=Zea mays GN=PG2C PE=2 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 1.5e-78
Identity = 165/372 (44.35%), Postives = 226/372 (60.75%), Query Frame = 1

Query: 21  TFNVVDFGAKPNVQTDSSQAFEAAWASACRARTAVSIYVPKGRFYVASLAFEGPCNNNDI 80
           +F++   GA  N +TDS++A + AWASAC      +I +PKG F V  L F GPC   D+
Sbjct: 40  SFDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGPLNFTGPCKG-DV 99

Query: 81  TIRIDGTLLAPSNYGVIANSGNWITFRRVDGVTLFG-GVLDAQGSGLWACKNS---KSSC 140
           TI+++G LLA ++     + GNWI   RVD + + G G LD QG  +W+ KNS   K  C
Sbjct: 100 TIQVNGNLLATTDLSQYKDHGNWIEILRVDNLVITGKGKLDGQGPAVWS-KNSCVKKYDC 159

Query: 141 PPGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQGLKVSAAGNSPNTDGIHVA 200
                SL      N  VSG+T LNS+ FH+ +  C+++  + + V+A G+SPNTDGIH+ 
Sbjct: 160 KILPNSLVMDFVNNGEVSGITLLNSKFFHMNMYKCKDMLIKDVNVTAPGDSPNTDGIHMG 219

Query: 201 LSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGISIGSLGREVQEDGVENVTV 260
            SS VTI N++IG GDDCISIGPGTS + I  V CGPGHGISIGSLGR   E  V ++ V
Sbjct: 220 DSSGVTITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINV 279

Query: 261 KSATFTNTQNGVRIKTWGKPSNGFARSVI-FQDIVMVNVENPIIIDQNYCPDNKGCPGQD 320
           K  T   T NGVRIK +   ++    S I +++I M +   PIIID  YCP+        
Sbjct: 280 KDCTLKKTANGVRIKAYEDAASVLTASKIHYENIKMEDSGYPIIIDMKYCPNKLCTANGA 339

Query: 321 SGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSDITLEDV--KLSCKNEAAEASCSHAE 380
           S V + DVT++NI GTS+T  A+   C++K PC+ +T++DV  K S  N    A C +A+
Sbjct: 340 SKVTVKDVTFKNITGTSSTPEAVNLLCTAKIPCTGVTMDDVNIKYSGTNNKTMAVCKNAK 399

Query: 381 GSAAGLVQPSSC 386
           GSA G ++  +C
Sbjct: 400 GSAKGCLKELAC 409

BLAST of Cucsa.138430.1 vs. TrEMBL
Match: A0A0A0M095_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G627460 PE=3 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 1.2e-223
Identity = 386/386 (100.00%), Postives = 386/386 (100.00%), Query Frame = 1

Query: 1   MAFHKTLFFPFLFLTLASASTFNVVDFGAKPNVQTDSSQAFEAAWASACRARTAVSIYVP 60
           MAFHKTLFFPFLFLTLASASTFNVVDFGAKPNVQTDSSQAFEAAWASACRARTAVSIYVP
Sbjct: 1   MAFHKTLFFPFLFLTLASASTFNVVDFGAKPNVQTDSSQAFEAAWASACRARTAVSIYVP 60

Query: 61  KGRFYVASLAFEGPCNNNDITIRIDGTLLAPSNYGVIANSGNWITFRRVDGVTLFGGVLD 120
           KGRFYVASLAFEGPCNNNDITIRIDGTLLAPSNYGVIANSGNWITFRRVDGVTLFGGVLD
Sbjct: 61  KGRFYVASLAFEGPCNNNDITIRIDGTLLAPSNYGVIANSGNWITFRRVDGVTLFGGVLD 120

Query: 121 AQGSGLWACKNSKSSCPPGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQGLK 180
           AQGSGLWACKNSKSSCPPGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQGLK
Sbjct: 121 AQGSGLWACKNSKSSCPPGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQGLK 180

Query: 181 VSAAGNSPNTDGIHVALSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGISIG 240
           VSAAGNSPNTDGIHVALSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGISIG
Sbjct: 181 VSAAGNSPNTDGIHVALSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGISIG 240

Query: 241 SLGREVQEDGVENVTVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENPIII 300
           SLGREVQEDGVENVTVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENPIII
Sbjct: 241 SLGREVQEDGVENVTVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENPIII 300

Query: 301 DQNYCPDNKGCPGQDSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSC 360
           DQNYCPDNKGCPGQDSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSC
Sbjct: 301 DQNYCPDNKGCPGQDSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSC 360

Query: 361 KNEAAEASCSHAEGSAAGLVQPSSCL 387
           KNEAAEASCSHAEGSAAGLVQPSSCL
Sbjct: 361 KNEAAEASCSHAEGSAAGLVQPSSCL 386

BLAST of Cucsa.138430.1 vs. TrEMBL
Match: A0A067EKY6_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g041993mg PE=3 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 3.3e-157
Identity = 260/372 (69.89%), Postives = 320/372 (86.02%), Query Frame = 1

Query: 15  TLASASTFNVVDFGAKPNVQTDSSQAFEAAWASACRARTAVSIYVPKGRFYVASLAFEGP 74
           +LA+  ++NVV FGAKP+ +TDS++AF +AWA+AC +  A +IYVP GRF++ ++AF+GP
Sbjct: 20  SLAAPPSYNVVSFGAKPDGRTDSTKAFLSAWANACGSPRAATIYVPPGRFFLRNVAFQGP 79

Query: 75  CNNNDITIRIDGTLLAPSNYGVIANSGNWITFRRVDGVTLFGGVLDAQGSGLWACKNSKS 134
           C NN ITIRIDGTL+APS+Y VI N+GNW+ F+ VDGVTL  G+LD QG+ LWACKNS  
Sbjct: 80  CKNNRITIRIDGTLVAPSDYRVIGNAGNWLFFQHVDGVTLNSGILDGQGTALWACKNSGK 139

Query: 135 SCPPGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQGLKVSAAGNSPNTDGIH 194
           +CP GATS+ FSN  NI ++GLTSLNSQM+H+V NGC NVK +G+KV A+GNSPNTDGIH
Sbjct: 140 NCPSGATSMGFSNCNNIAINGLTSLNSQMYHVVFNGCNNVKLKGVKVLASGNSPNTDGIH 199

Query: 195 VALSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGISIGSLGREVQEDGVENV 254
           V LSS VTILN+ I TGDDC+SIGPGTSNLWIENVACGPGHGISIGSLG+++ E GV+NV
Sbjct: 200 VQLSSDVTILNTRISTGDDCVSIGPGTSNLWIENVACGPGHGISIGSLGKDLNEAGVQNV 259

Query: 255 TVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENPIIIDQNYCPDNKGCPGQ 314
           TVK+ TFT TQNG+RIK+WG+PSNGFAR+++F++ +M NV+NPIIIDQNYCPDN GCPGQ
Sbjct: 260 TVKTVTFTGTQNGLRIKSWGRPSNGFARNILFRNAIMKNVQNPIIIDQNYCPDNIGCPGQ 319

Query: 315 DSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSCKNEAAEASCSHAEG 374
            SGVKISDVTY+++HGTSAT+VA+ FDCSSK+PCS I LEDVKL+ KN+ A ASCSHA+G
Sbjct: 320 VSGVKISDVTYQDVHGTSATEVAVNFDCSSKYPCSRIRLEDVKLTYKNQPAAASCSHADG 379

Query: 375 SAAGLVQPSSCL 387
           SA+GLVQP  CL
Sbjct: 380 SASGLVQPKGCL 391

BLAST of Cucsa.138430.1 vs. TrEMBL
Match: M5VW01_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007271mg PE=3 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 2.6e-154
Identity = 264/370 (71.35%), Postives = 311/370 (84.05%), Query Frame = 1

Query: 17  ASASTFNVVDFGAKPNVQTDSSQAFEAAWASACRARTAVSIYVPKGRFYVASLAFEGPCN 76
           A+  T++V   GA  + +TDS++ F +AW+ AC +     IYVP GRF V + AF GPC 
Sbjct: 6   AADLTYSVTSLGAIADGKTDSTKVFLSAWSKACASVQPAVIYVPAGRFLVQNAAFSGPCK 65

Query: 77  NNDITIRIDGTLLAPSNYGVIANSGNWITFRRVDGVTLFGGVLDAQGSGLWACKNSKSSC 136
           NN IT RIDGTL+APS+Y VI N+GNWI F  V+GVT+ GG+LD QG+GLWACK S  SC
Sbjct: 66  NNAITFRIDGTLVAPSDYRVIGNAGNWILFEHVNGVTISGGLLDGQGTGLWACKASTKSC 125

Query: 137 PPGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQGLKVSAAGNSPNTDGIHVA 196
           P GAT+L FS+S NI+V+GL SLNSQMFHIVINGCQNVK QG+KVSA+GNSPNTDGIHV 
Sbjct: 126 PRGATTLGFSSSNNIVVNGLASLNSQMFHIVINGCQNVKMQGVKVSASGNSPNTDGIHVQ 185

Query: 197 LSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGISIGSLGREVQEDGVENVTV 256
           +SS VTILNS I TGDDC+SIGPGT+NLWIENVACGPGHGISIGSLG++ QEDGV+NVTV
Sbjct: 186 MSSEVTILNSKIATGDDCVSIGPGTTNLWIENVACGPGHGISIGSLGKDSQEDGVQNVTV 245

Query: 257 KSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENPIIIDQNYCPDNKGCPGQDS 316
           K+ TFT TQNGVRIK+WG+PS GFAR+++FQ IV VNV+NPI+IDQNYCP NKGCPGQ S
Sbjct: 246 KTVTFTGTQNGVRIKSWGRPSTGFARNILFQHIVNVNVQNPIVIDQNYCPGNKGCPGQVS 305

Query: 317 GVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSCKNEAAEASCSHAEGSA 376
           GVKISDV Y++IHGTSATQVA+KFDCSSK+PCS I LEDVKL+ KN+AAEASC +A G+A
Sbjct: 306 GVKISDVIYQDIHGTSATQVAVKFDCSSKYPCSGIKLEDVKLTYKNQAAEASCKYAGGTA 365

Query: 377 AGLVQPSSCL 387
           AG VQP+SCL
Sbjct: 366 AGTVQPTSCL 375

BLAST of Cucsa.138430.1 vs. TrEMBL
Match: U5FKL6_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0017s03850g PE=3 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 3.4e-154
Identity = 264/383 (68.93%), Postives = 316/383 (82.51%), Query Frame = 1

Query: 6   TLFFP--FLFLTLASASTFNVVDFGAKPNVQTDSSQAFEAAWASACRARTAVSIYVPKGR 65
           TLFF   F F  LA A+ F+V+ +GA P+ +TDS++AF AAWA AC +    +I VPKG 
Sbjct: 15  TLFFIIFFAFSPLAKAAQFSVLSYGATPDGKTDSTKAFAAAWAQACASTQPATISVPKGS 74

Query: 66  FYVASLAFEGPCNNNDITIRIDGTLLAPSNYGVIANSGNWITFRRVDGVTLFGGVLDAQG 125
           F +  + F+GPCNN  I +RIDGTL+APS+Y VI N+ NW+ F  V+GVT+ GG LD QG
Sbjct: 75  FSLGQVRFQGPCNNRAILVRIDGTLVAPSDYKVIGNAKNWLIFEHVNGVTVSGGTLDGQG 134

Query: 126 SGLWACKNSKSSCPPGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQGLKVSA 185
           +GLW+CKNS   CP GATSLEFSNS NI ++GL SLNSQ+FHIVINGCQNVK QG+KVSA
Sbjct: 135 AGLWSCKNSGKGCPRGATSLEFSNSNNIAITGLASLNSQLFHIVINGCQNVKVQGVKVSA 194

Query: 186 AGNSPNTDGIHVALSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGISIGSLG 245
           AGNSPNTDGIHV  S+ VTILNS IGTGDDC+SIGPGTS+LWIENVACGPGHGISIGSLG
Sbjct: 195 AGNSPNTDGIHVQSSTGVTILNSRIGTGDDCVSIGPGTSSLWIENVACGPGHGISIGSLG 254

Query: 246 REVQEDGVENVTVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENPIIIDQN 305
           +E QE GV+NVTVK+ TFT T+NG+RIK+WG+PSNGFAR ++FQ  VM NV+NPI+IDQN
Sbjct: 255 KESQEAGVQNVTVKTTTFTGTENGLRIKSWGRPSNGFARDILFQHAVMNNVQNPIVIDQN 314

Query: 306 YCPDNKGCPGQDSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSCKNE 365
           YCP  K CPGQ SGVKISDVTY++IHG+SAT+VA+KFDCS K+PC+ I LEDVKL+ KN+
Sbjct: 315 YCPGEKNCPGQVSGVKISDVTYQDIHGSSATEVAVKFDCSKKYPCTGIKLEDVKLTYKNQ 374

Query: 366 AAEASCSHAEGSAAGLVQPSSCL 387
            AEASCS+A G A+GLVQP+SCL
Sbjct: 375 PAEASCSNAGGVASGLVQPTSCL 397

BLAST of Cucsa.138430.1 vs. TrEMBL
Match: A0A059AUR6_EUCGR (Uncharacterized protein (Fragment) OS=Eucalyptus grandis GN=EUGRSUZ_H00254 PE=3 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 4.5e-154
Identity = 258/365 (70.68%), Postives = 310/365 (84.93%), Query Frame = 1

Query: 22  FNVVDFGAKPNVQTDSSQAFEAAWASACRARTAVSIYVPKGRFYVASLAFEGPCNNNDIT 81
           +NVV  GAK + +TDSS+AF++AWASAC +  A S+YVP+GRF V S +F GPC N+ IT
Sbjct: 2   YNVVSLGAKADGKTDSSKAFQSAWASACGSTRAASVYVPQGRFLVKSASFNGPCKNSAIT 61

Query: 82  IRIDGTLLAPSNYGVIANSGNWITFRRVDGVTLFGGVLDAQGSGLWACKNSKSSCPPGAT 141
           +RIDGTL+APSNY V  N+  WI FR V GV++ GG LD QG+GLWACK S  SCP GAT
Sbjct: 62  VRIDGTLVAPSNYNVNGNARRWIVFRHVTGVSMIGGTLDGQGTGLWACKLSHKSCPIGAT 121

Query: 142 SLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQGLKVSAAGNSPNTDGIHVALSSTV 201
           +L  SNS+NI+V GL SLNSQMFHIVINGCQNVK QG+KVSA+G SPNTDGIHV  S+ V
Sbjct: 122 NLLISNSQNILVRGLRSLNSQMFHIVINGCQNVKMQGVKVSASGTSPNTDGIHVGASTGV 181

Query: 202 TILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGISIGSLGREVQEDGVENVTVKSATF 261
           TILNS IGTGDDC+SIGPG++NLWIENVACGPGHGISIGSLG+  QEDGV+NVTVK+ TF
Sbjct: 182 TILNSKIGTGDDCVSIGPGSNNLWIENVACGPGHGISIGSLGKTQQEDGVQNVTVKTVTF 241

Query: 262 TNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENPIIIDQNYCPDNKGCPGQDSGVKIS 321
           T TQNGVRIK+WG+PSNGFAR+++FQ  +M NV+NPIIIDQNYCP N GCPGQ SGV++S
Sbjct: 242 TGTQNGVRIKSWGRPSNGFARNILFQHAIMNNVQNPIIIDQNYCPHNSGCPGQASGVRVS 301

Query: 322 DVTYRNIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSCKNEAAEASCSHAEGSAAGLVQ 381
           DVTY++IHGTSAT VA+KFDCSSK+PC+ I ++DVKL+ +N+ A ASC+HA+G+A+GLVQ
Sbjct: 302 DVTYQDIHGTSATSVAVKFDCSSKYPCTGIRMQDVKLTYRNKVATASCAHADGTASGLVQ 361

Query: 382 PSSCL 387
           PSSCL
Sbjct: 362 PSSCL 366

BLAST of Cucsa.138430.1 vs. TAIR10
Match: AT3G59850.1 (AT3G59850.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 502.3 bits (1292), Expect = 2.7e-142
Identity = 236/387 (60.98%), Postives = 301/387 (77.78%), Query Frame = 1

Query: 4   HKTLFFPFLFL---TLASASTFNVVDFGAKPNVQTDSSQAFEAAWASACRARTAVSIYVP 63
           H +L    LFL   + +SA T+N++ +GAKP+ +TDS++AF   WA AC +   V+I VP
Sbjct: 2   HSSLLIVLLFLLSVSSSSAQTYNILSYGAKPDGKTDSTKAFTVLWAKACASVKPVTILVP 61

Query: 64  KGRFYVASLAFEGP-CNNNDITIRIDGTLLAPSNYGVIANSGNWITFRRVDGVTLFGGVL 123
           KGRF + S+ F+G  C    +T RI GTL+APS+Y VI     WI F+ +DG++++GGVL
Sbjct: 62  KGRFLLRSIIFDGSKCKRKSVTFRIQGTLVAPSDYRVIGKENYWILFQHLDGISVYGGVL 121

Query: 124 DAQGSGLWACKNSKSSCPPGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQGL 183
           DAQG+ LW+CK S  +CP GATS+ F +S+N+++SGLTSLNSQMFH+ INGC NVK  G+
Sbjct: 122 DAQGASLWSCKKSGKNCPSGATSIGFQSSRNVVISGLTSLNSQMFHVAINGCSNVKLDGV 181

Query: 184 KVSAAGNSPNTDGIHVALSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGISI 243
           KVSA GNSPNTDGIHV  SSTV+ILNS I TGDDC+SIGPGT+ LWIENVACGPGHGISI
Sbjct: 182 KVSADGNSPNTDGIHVQSSSTVSILNSKISTGDDCVSIGPGTNGLWIENVACGPGHGISI 241

Query: 244 GSLGREVQEDGVENVTVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENPII 303
           GSLG+E  E GV+N+TVK+ATFT T+NGVRIK+W +PSNGFA+++ FQ  VM NV+NPI+
Sbjct: 242 GSLGKESVEVGVQNITVKTATFTGTENGVRIKSWARPSNGFAKNIRFQHCVMNNVQNPIV 301

Query: 304 IDQNYCPDNKGCPGQDSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSDITLEDVKLS 363
           IDQNYCP N+ CP Q SG+KISDV + +IHGTSAT+V +K DCSSK PC+ I ++DVKL+
Sbjct: 302 IDQNYCPGNENCPNQVSGIKISDVMFFDIHGTSATEVGVKLDCSSKKPCTGIRIQDVKLT 361

Query: 364 CKNEAAEASCSHAEGSAAGLVQPSSCL 387
            +N+ A   CSHA GS AG  +P+SCL
Sbjct: 362 YRNKPATTDCSHAGGSEAGFQRPNSCL 388

BLAST of Cucsa.138430.1 vs. TAIR10
Match: AT2G43870.1 (AT2G43870.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 489.6 bits (1259), Expect = 1.8e-138
Identity = 234/381 (61.42%), Postives = 299/381 (78.48%), Query Frame = 1

Query: 7   LFFPFLFLTLASASTFNVVDFGAKPNVQTDSSQAFEAAWASACRARTAVSIYVPKGRFYV 66
           + F F F++  SA ++NV+ FGAKP+ +TD+++AF A W +AC +   V+I VPKGRF +
Sbjct: 6   VLFVFFFISSCSAQSYNVLSFGAKPDGKTDATKAFMAVWQTACASSRPVTIVVPKGRFLL 65

Query: 67  ASLAFEGP-CNNNDITIRIDGTLLAPSNYGVIANSGNWITFRRVDGVTLFGGVLDAQGSG 126
            S+ F+G  C    +T RIDGTL+AP++Y VI N   WI F+ +DG+T++GGVLDA+G+ 
Sbjct: 66  RSVTFDGSKCKPKPVTFRIDGTLVAPADYRVIGNEDYWIFFQHLDGITVYGGVLDARGAS 125

Query: 127 LWACKNSKSSCPPGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQGLKVSAAG 186
           LW CK S  +CP GAT++ F +S N++VSGLTSLNSQMFH+VINGC NVK QG+KV AAG
Sbjct: 126 LWDCKKSGKNCPSGATTIGFQSSSNVVVSGLTSLNSQMFHVVINGCNNVKLQGVKVLAAG 185

Query: 187 NSPNTDGIHVALSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGISIGSLGRE 246
           NSPNTDGIHV  SS+V+I N+ I TGDDC+SIGPGT+ LWIENVACGPGHGISIGSLG++
Sbjct: 186 NSPNTDGIHVQSSSSVSIFNTKISTGDDCVSIGPGTNGLWIENVACGPGHGISIGSLGKD 245

Query: 247 VQEDGVENVTVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENPIIIDQNYC 306
             E GV+NVTVK+ TFT T NGVRIK+W +PS+GFA+++ FQ  VM NVENPIIIDQNYC
Sbjct: 246 SVESGVQNVTVKTVTFTGTDNGVRIKSWARPSSGFAKNIRFQHCVMNNVENPIIIDQNYC 305

Query: 307 PDNKGCPGQDSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSCKNEAA 366
           PD+  CP Q SG+KISDV + +IHGTSAT+V +K DCSSK PC+ I LEDVKL+ +N+ A
Sbjct: 306 PDH-DCPRQVSGIKISDVLFVDIHGTSATEVGVKLDCSSKKPCTGIRLEDVKLTYQNKPA 365

Query: 367 EASCSHAEGSAAGLVQPSSCL 387
            ++C+HA G  AG  QP +CL
Sbjct: 366 ASACTHAGGIEAGFFQP-NCL 384

BLAST of Cucsa.138430.1 vs. TAIR10
Match: AT1G05660.1 (AT1G05660.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 436.8 bits (1122), Expect = 1.4e-122
Identity = 202/377 (53.58%), Postives = 278/377 (73.74%), Query Frame = 1

Query: 11  FLFLTLASASTFNVVDFGAKPNVQTDSSQAFEAAWASACRARTAVSIYVPKGRFYVASLA 70
           F+ +++++++ FNVV FGAKP+  TDS+ AF  AW  AC + ++ ++ VPKG F +  + 
Sbjct: 17  FIDVSISASNVFNVVSFGAKPDGVTDSTGAFLKAWQGACVSASSATVVVPKGTFLLKVIT 76

Query: 71  FEGPCNNNDITIRIDGTLLAPSNYGVIANSGNWITFRRVDGVTLFGGVLDAQGSGLWACK 130
           F GPC +  IT ++ GT++AP +Y    NSG WI F +V+  +L GG  DA+ +G W+C+
Sbjct: 77  FGGPCKS-KITFQVAGTVIAPEDYRTFGNSGFWILFNKVNRFSLVGGTFDARANGFWSCR 136

Query: 131 NSKSSCPPGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQGLKVSAAGNSPNT 190
            S  +CPPG  S+ F+++K++++SG+ S+NSQ+ H+ +NGC NV  + +K+ A GNSPNT
Sbjct: 137 KSGQNCPPGVRSISFNSAKDVIISGVKSMNSQVTHMTLNGCTNVVVRNVKLVAPGNSPNT 196

Query: 191 DGIHVALSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGISIGSLGREVQEDG 250
           DG HV  S+ VT   S + TGDDC++IGPGT NL I  +ACGPGHG+SIGSL +E++EDG
Sbjct: 197 DGFHVQHSTGVTFTGSTVQTGDDCVAIGPGTRNLLITKLACGPGHGVSIGSLAKELKEDG 256

Query: 251 VENVTVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENPIIIDQNYCPDNKG 310
           VENVTV S+ FT +QNGVRIK+W +PSNGF R+V FQD+VM NVENPIIIDQNYCP ++G
Sbjct: 257 VENVTVSSSVFTGSQNGVRIKSWARPSNGFVRTVFFQDLVMKNVENPIIIDQNYCPTHEG 316

Query: 311 CPGQDSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSC-KNEAAEASC 370
           CP + SGVKIS VTY+NI GTSATQ AMK  CS   PC+ ITL+D+KL+  K   A + C
Sbjct: 317 CPNEYSGVKISQVTYKNIQGTSATQEAMKLVCSKSSPCTGITLQDIKLTYNKGTPATSFC 376

Query: 371 SHAEGSAAGLVQPSSCL 387
            +A G + G++QP+SCL
Sbjct: 377 FNAVGKSLGVIQPTSCL 392

BLAST of Cucsa.138430.1 vs. TAIR10
Match: AT2G43890.1 (AT2G43890.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 424.1 bits (1089), Expect = 9.3e-119
Identity = 201/382 (52.62%), Postives = 272/382 (71.20%), Query Frame = 1

Query: 7   LFFPFLFLTLAS--ASTFNVVDFGAKPNVQTDSSQAFEAAWASACRARTAVSIYVPKGRF 66
           +FF    L  +S  AS +NVV FGAKP+ +TDS++AF  AW +ACR+  AV++ VP+G F
Sbjct: 12  MFFSSFLLMKSSTAASNYNVVSFGAKPDGRTDSTKAFLGAWQAACRSAAAVTVTVPRGSF 71

Query: 67  YVASLAFEGPCNNNDITIRIDGTLLAPSNYGVIANSGNWITFRRVDGVTLFGGVLDAQGS 126
            +  + F GPC +  IT +I GT++APS+Y  + NSG WI F +V+ +++ GG LDA+G+
Sbjct: 72  LLKPVEFRGPCRSR-ITFQIYGTIVAPSDYRGLGNSGYWILFVKVNRISIIGGTLDARGA 131

Query: 127 GLWACKNSKSSCPPGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQGLKVSAA 186
             WAC+ S  SCP GA S+ F+ + +++VSGLTS+NSQ  H+VIN C NV  + +K+ A 
Sbjct: 132 SFWACRKSGKSCPVGARSMTFNWANDVVVSGLTSINSQTTHLVINSCNNVIVRKVKLVAP 191

Query: 187 GNSPNTDGIHVALSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGISIGSLGR 246
             SPNTDG+HV  S+ VT+ +    TGDDCISIGPGT NL++  + CGPGHGISIGSLGR
Sbjct: 192 DQSPNTDGLHVQGSAGVTVTDGTFHTGDDCISIGPGTRNLYMSKLNCGPGHGISIGSLGR 251

Query: 247 EVQEDGVENVTVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENPIIIDQNY 306
           +  E GVEN+T+ ++ F+ + NGVRIKTW + S GF R+V+FQ+++M NV+NPII+DQNY
Sbjct: 252 DANEAGVENITLINSVFSGSDNGVRIKTWARQSTGFVRNVLFQNLIMKNVQNPIIVDQNY 311

Query: 307 CPDNKGCPGQDSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSCKNEA 366
           CP N+GCP Q SGVKIS V YRNI GTS TQ A+ FDCS   PC  I L D+KL+    +
Sbjct: 312 CPSNQGCPKQGSGVKISQVVYRNIQGTSRTQQALTFDCSRSNPCQAIRLHDIKLTFNGRS 371

Query: 367 AEASCSHAEGSAAGLVQPSSCL 387
           A ++C + +G  AG+V P  CL
Sbjct: 372 ATSTCKNIKGVKAGVVMPQGCL 392

BLAST of Cucsa.138430.1 vs. TAIR10
Match: AT1G05650.1 (AT1G05650.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 421.4 bits (1082), Expect = 6.0e-118
Identity = 199/386 (51.55%), Postives = 273/386 (70.73%), Query Frame = 1

Query: 9   FPFLFLTL------ASAS-TFNVVDFGAKPNVQTDSSQAFEAAWASACRARTAVSIYVPK 68
           FP +F  L      +SAS  FNVV FGAKP+  TDS+ AF  AW  AC +  + ++ VP 
Sbjct: 8   FPLIFTLLTFIDVSSSASIVFNVVSFGAKPDGVTDSTAAFLKAWQGACGSAASATVVVPT 67

Query: 69  GRFYVASLAFEGPCNNNDITIRIDGTLLAPSNYGVIANSGNWITFRRVDGVTLFGGVLDA 128
           G F +  + F GPC +  IT ++ GT++AP +Y    NSG+WI F +V+  +L GG  DA
Sbjct: 68  GTFLLKVITFGGPCKSK-ITFQVTGTVVAPEDYRTFGNSGSWILFNKVNRFSLVGGTFDA 127

Query: 129 QGSGLWACKNSKSSCPPGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQGLKV 188
           +GSG W+C+ S  +CPPG  S+ F+++K++++SG+ S+NSQ+ H+ +NGC NV  + +++
Sbjct: 128 RGSGFWSCRKSGQNCPPGVRSISFNSAKDVIISGVKSMNSQVSHMTLNGCTNVAVRNIRL 187

Query: 189 SAAGNSPNTDGIHVALSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGISIGS 248
            A G+SPNTDG  V  S+ VT+  S + TGDDC++IG GT N  I  +ACGPGHG+SIGS
Sbjct: 188 VAPGDSPNTDGFTVQFSTGVTLTGSTVQTGDDCVAIGQGTRNFLISKLACGPGHGVSIGS 247

Query: 249 LGREVQEDGVENVTVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENPIIID 308
           L +++ EDGVENVTV S+ FT +QNGVRIK+W +PS GF R+V FQ+++M NV+NPIIID
Sbjct: 248 LAKQLNEDGVENVTVSSSVFTGSQNGVRIKSWARPSTGFVRNVFFQNLIMRNVQNPIIID 307

Query: 309 QNYCPDNKGCPGQDSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSC- 368
           QNYCP N+GCP + SGVKI+ VTY+NI GTSATQ AMK  CS   PC+ ITL+D+KL+  
Sbjct: 308 QNYCPSNQGCPTEHSGVKITQVTYKNIQGTSATQEAMKLVCSKSNPCTGITLQDIKLTYN 367

Query: 369 KNEAAEASCSHAEGSAAGLVQPSSCL 387
           K   A + C +A G   G++QP+SCL
Sbjct: 368 KGTPATSLCFNAVGKNLGVIQPTSCL 392

BLAST of Cucsa.138430.1 vs. NCBI nr
Match: gi|449442581|ref|XP_004139060.1| (PREDICTED: polygalacturonase-like [Cucumis sativus])

HSP 1 Score: 783.5 bits (2022), Expect = 1.7e-223
Identity = 386/386 (100.00%), Postives = 386/386 (100.00%), Query Frame = 1

Query: 1   MAFHKTLFFPFLFLTLASASTFNVVDFGAKPNVQTDSSQAFEAAWASACRARTAVSIYVP 60
           MAFHKTLFFPFLFLTLASASTFNVVDFGAKPNVQTDSSQAFEAAWASACRARTAVSIYVP
Sbjct: 1   MAFHKTLFFPFLFLTLASASTFNVVDFGAKPNVQTDSSQAFEAAWASACRARTAVSIYVP 60

Query: 61  KGRFYVASLAFEGPCNNNDITIRIDGTLLAPSNYGVIANSGNWITFRRVDGVTLFGGVLD 120
           KGRFYVASLAFEGPCNNNDITIRIDGTLLAPSNYGVIANSGNWITFRRVDGVTLFGGVLD
Sbjct: 61  KGRFYVASLAFEGPCNNNDITIRIDGTLLAPSNYGVIANSGNWITFRRVDGVTLFGGVLD 120

Query: 121 AQGSGLWACKNSKSSCPPGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQGLK 180
           AQGSGLWACKNSKSSCPPGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQGLK
Sbjct: 121 AQGSGLWACKNSKSSCPPGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQGLK 180

Query: 181 VSAAGNSPNTDGIHVALSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGISIG 240
           VSAAGNSPNTDGIHVALSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGISIG
Sbjct: 181 VSAAGNSPNTDGIHVALSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGISIG 240

Query: 241 SLGREVQEDGVENVTVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENPIII 300
           SLGREVQEDGVENVTVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENPIII
Sbjct: 241 SLGREVQEDGVENVTVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENPIII 300

Query: 301 DQNYCPDNKGCPGQDSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSC 360
           DQNYCPDNKGCPGQDSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSC
Sbjct: 301 DQNYCPDNKGCPGQDSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSC 360

Query: 361 KNEAAEASCSHAEGSAAGLVQPSSCL 387
           KNEAAEASCSHAEGSAAGLVQPSSCL
Sbjct: 361 KNEAAEASCSHAEGSAAGLVQPSSCL 386

BLAST of Cucsa.138430.1 vs. NCBI nr
Match: gi|568880467|ref|XP_006493140.1| (PREDICTED: polygalacturonase-like [Citrus sinensis])

HSP 1 Score: 564.3 bits (1453), Expect = 1.6e-157
Identity = 261/372 (70.16%), Postives = 320/372 (86.02%), Query Frame = 1

Query: 15  TLASASTFNVVDFGAKPNVQTDSSQAFEAAWASACRARTAVSIYVPKGRFYVASLAFEGP 74
           +LA+  ++NVV FGAKP+ +TDS++AF +AWA+AC +  A +IYVP GRF++ ++AF+GP
Sbjct: 24  SLAAPPSYNVVSFGAKPDGRTDSTKAFLSAWANACGSPRAATIYVPPGRFFLRNVAFQGP 83

Query: 75  CNNNDITIRIDGTLLAPSNYGVIANSGNWITFRRVDGVTLFGGVLDAQGSGLWACKNSKS 134
           C NN ITIRIDGTL+APS+Y VI N+GNW+ F+ VDGVTL  G+LD QG+ LWACKNS  
Sbjct: 84  CKNNRITIRIDGTLVAPSDYRVIGNAGNWLFFQHVDGVTLNSGILDGQGTALWACKNSGK 143

Query: 135 SCPPGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQGLKVSAAGNSPNTDGIH 194
           +CP GATS+ FSN  NI ++GLTSLNSQM+H+V NGC NVK +G+KV A+GNSPNTDGIH
Sbjct: 144 NCPSGATSMGFSNCNNIAINGLTSLNSQMYHVVFNGCNNVKLKGVKVLASGNSPNTDGIH 203

Query: 195 VALSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGISIGSLGREVQEDGVENV 254
           V LSS VTILN+ I TGDDC+SIGPGTSNLWIENVACGPGHGISIGSLG+++ E GV+NV
Sbjct: 204 VQLSSDVTILNTRISTGDDCVSIGPGTSNLWIENVACGPGHGISIGSLGKDLNEAGVQNV 263

Query: 255 TVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENPIIIDQNYCPDNKGCPGQ 314
           TVK+ TFT TQNG+RIK+WG+PSNGFAR+++FQ+ +M NV+NPIIIDQNYCPDN GCPGQ
Sbjct: 264 TVKTVTFTGTQNGLRIKSWGRPSNGFARNILFQNAIMKNVQNPIIIDQNYCPDNIGCPGQ 323

Query: 315 DSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSCKNEAAEASCSHAEG 374
            SGVKISDVTY+++HGTSAT+VA+ FDCSSK+PCS I LEDVKL+ KN+ A ASCSHA+G
Sbjct: 324 VSGVKISDVTYQDVHGTSATEVAVNFDCSSKYPCSRIRLEDVKLTYKNQPAAASCSHADG 383

Query: 375 SAAGLVQPSSCL 387
           SA+GLVQP  CL
Sbjct: 384 SASGLVQPKGCL 395

BLAST of Cucsa.138430.1 vs. NCBI nr
Match: gi|641832559|gb|KDO51591.1| (hypothetical protein CISIN_1g041993mg [Citrus sinensis])

HSP 1 Score: 562.8 bits (1449), Expect = 4.8e-157
Identity = 260/372 (69.89%), Postives = 320/372 (86.02%), Query Frame = 1

Query: 15  TLASASTFNVVDFGAKPNVQTDSSQAFEAAWASACRARTAVSIYVPKGRFYVASLAFEGP 74
           +LA+  ++NVV FGAKP+ +TDS++AF +AWA+AC +  A +IYVP GRF++ ++AF+GP
Sbjct: 20  SLAAPPSYNVVSFGAKPDGRTDSTKAFLSAWANACGSPRAATIYVPPGRFFLRNVAFQGP 79

Query: 75  CNNNDITIRIDGTLLAPSNYGVIANSGNWITFRRVDGVTLFGGVLDAQGSGLWACKNSKS 134
           C NN ITIRIDGTL+APS+Y VI N+GNW+ F+ VDGVTL  G+LD QG+ LWACKNS  
Sbjct: 80  CKNNRITIRIDGTLVAPSDYRVIGNAGNWLFFQHVDGVTLNSGILDGQGTALWACKNSGK 139

Query: 135 SCPPGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQGLKVSAAGNSPNTDGIH 194
           +CP GATS+ FSN  NI ++GLTSLNSQM+H+V NGC NVK +G+KV A+GNSPNTDGIH
Sbjct: 140 NCPSGATSMGFSNCNNIAINGLTSLNSQMYHVVFNGCNNVKLKGVKVLASGNSPNTDGIH 199

Query: 195 VALSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGISIGSLGREVQEDGVENV 254
           V LSS VTILN+ I TGDDC+SIGPGTSNLWIENVACGPGHGISIGSLG+++ E GV+NV
Sbjct: 200 VQLSSDVTILNTRISTGDDCVSIGPGTSNLWIENVACGPGHGISIGSLGKDLNEAGVQNV 259

Query: 255 TVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENPIIIDQNYCPDNKGCPGQ 314
           TVK+ TFT TQNG+RIK+WG+PSNGFAR+++F++ +M NV+NPIIIDQNYCPDN GCPGQ
Sbjct: 260 TVKTVTFTGTQNGLRIKSWGRPSNGFARNILFRNAIMKNVQNPIIIDQNYCPDNIGCPGQ 319

Query: 315 DSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSCKNEAAEASCSHAEG 374
            SGVKISDVTY+++HGTSAT+VA+ FDCSSK+PCS I LEDVKL+ KN+ A ASCSHA+G
Sbjct: 320 VSGVKISDVTYQDVHGTSATEVAVNFDCSSKYPCSRIRLEDVKLTYKNQPAAASCSHADG 379

Query: 375 SAAGLVQPSSCL 387
           SA+GLVQP  CL
Sbjct: 380 SASGLVQPKGCL 391

BLAST of Cucsa.138430.1 vs. NCBI nr
Match: gi|470101431|ref|XP_004287176.1| (PREDICTED: polygalacturonase [Fragaria vesca subsp. vesca])

HSP 1 Score: 555.8 bits (1431), Expect = 5.8e-155
Identity = 264/391 (67.52%), Postives = 323/391 (82.61%), Query Frame = 1

Query: 1   MAFHKTLF-----FPFLFLTLASASTFNVVDFGAKPNVQTDSSQAFEAAWASACRARTAV 60
           MA H++L      F F++  +A A T++V   GAKP+ +TDS++AF +AWA AC +    
Sbjct: 1   MANHRSLILLSFLFIFIYSAIAKAVTYSVTTLGAKPDGKTDSTKAFLSAWAKACASVKPP 60

Query: 61  SIYVPKGRFYVASLAFEGPCNNNDITIRIDGTLLAPSNYGVIANSGNWITFRRVDGVTLF 120
            IYVP GRF + +  F GPC N+ IT RI GTL+APS+Y VI N+GNW+ F+ V+GVT+ 
Sbjct: 61  VIYVPAGRFLLRNAVFSGPCKNSRITFRIAGTLVAPSDYRVIGNAGNWLLFQHVNGVTIS 120

Query: 121 GGVLDAQGSGLWACKNSKSSCPPGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVK 180
           GG+LD QG+GLWACKNS  SCP GAT+L FSNS NI+V GL SLNSQMFHIV+NGC NVK
Sbjct: 121 GGILDGQGTGLWACKNSGKSCPSGATTLSFSNSNNIVVKGLVSLNSQMFHIVVNGCHNVK 180

Query: 181 AQGLKVSAAGNSPNTDGIHVALSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGH 240
            QG+KVSA+GNSPNTDGIHV +SS VTILNS I TGDDC+SIGPGTSNLWIENVACGPGH
Sbjct: 181 MQGVKVSASGNSPNTDGIHVQMSSGVTILNSKISTGDDCVSIGPGTSNLWIENVACGPGH 240

Query: 241 GISIGSLGREVQEDGVENVTVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVE 300
           GISIGSLG++++E GV+NVTVK+ TFTNTQNGVRIK+WG+ S GFAR+++FQ  VM NV+
Sbjct: 241 GISIGSLGKDLKEAGVQNVTVKTVTFTNTQNGVRIKSWGRASTGFARNILFQHCVMNNVQ 300

Query: 301 NPIIIDQNYCPDNKGCPGQDSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSDITLED 360
           NP+++DQ YCPDNKGCPG+ SGVKISDVTY++IHGTSAT+VA+KFDCSSK+PCS I LE+
Sbjct: 301 NPVVVDQKYCPDNKGCPGEVSGVKISDVTYQDIHGTSATEVAVKFDCSSKYPCSRIRLEN 360

Query: 361 VKLSCKNEAAEASCSHAEGSAAGLVQPSSCL 387
           VKL+ KNEAA ASCS+A G++AG VQP+SCL
Sbjct: 361 VKLTYKNEAALASCSNAGGTSAGTVQPTSCL 391

BLAST of Cucsa.138430.1 vs. NCBI nr
Match: gi|502143417|ref|XP_004505336.1| (PREDICTED: polygalacturonase-like [Cicer arietinum])

HSP 1 Score: 553.9 bits (1426), Expect = 2.2e-154
Identity = 262/383 (68.41%), Postives = 314/383 (81.98%), Query Frame = 1

Query: 6   TLFFPFLFLTLASASTFNVVDFGAKPNVQTDSSQAFEAAWASACRARTAVSIYVPKGRFY 65
           T+ +  L  + A AST+NV+ FGAKPN +TDS++AF  AW  AC +   VSIYVP+G+F 
Sbjct: 15  TILYCILISSTAKASTYNVIQFGAKPNGKTDSTKAFLNAWTKACASPNPVSIYVPQGKFL 74

Query: 66  VASLAFEGPCNNNDITIRIDGTLLAPSNYGVIANSGNWITFRRVDGVTLFGGVLDAQGSG 125
           + +  F G C N  I+I IDGTL+APS+Y +  N+G W+ F+ VDGV++ GGVLD QG+ 
Sbjct: 75  LGAATFNGKCTNKAISIIIDGTLVAPSDYRLTGNAGYWLKFQHVDGVSIRGGVLDGQGTS 134

Query: 126 LWACKNS--KSSCPPGATSLEFSNSKNIMVSGLTSLNSQMFHIVINGCQNVKAQGLKVSA 185
           LW CKNS   S+CP G T+LEFSNSKNIM+SGLTS+NSQMFHIV NGCQNV AQG+K+ A
Sbjct: 135 LWDCKNSGKSSNCPTGVTTLEFSNSKNIMISGLTSMNSQMFHIVFNGCQNVMAQGVKIMA 194

Query: 186 AGNSPNTDGIHVALSSTVTILNSIIGTGDDCISIGPGTSNLWIENVACGPGHGISIGSLG 245
           AGNSPNTDGIHV +SS VTIL S I TGDDCISIGPGTSNLWIEN+ACGPGHGISIGSLG
Sbjct: 195 AGNSPNTDGIHVQMSSHVTILKSNIRTGDDCISIGPGTSNLWIENIACGPGHGISIGSLG 254

Query: 246 REVQEDGVENVTVKSATFTNTQNGVRIKTWGKPSNGFARSVIFQDIVMVNVENPIIIDQN 305
            E+ E GV+NVTVK+ TF+ TQNGVRIK+WG+PSNGF R+V FQD +MVNV+NPI+IDQN
Sbjct: 255 WELNEPGVQNVTVKTVTFSGTQNGVRIKSWGRPSNGFVRNVFFQDAIMVNVQNPIVIDQN 314

Query: 306 YCPDNKGCPGQDSGVKISDVTYRNIHGTSATQVAMKFDCSSKFPCSDITLEDVKLSCKNE 365
           YCP+NK CPGQ SG+KISDVTY +IHGTSATQVA+KFDCS K+PCS + L+DVKL+ KN+
Sbjct: 315 YCPNNKDCPGQASGIKISDVTYEDIHGTSATQVAVKFDCSYKYPCSGLKLKDVKLTYKNQ 374

Query: 366 AAEASCSHAEGSAAGLVQPSSCL 387
            AEASC+HA G+A GLVQP SCL
Sbjct: 375 VAEASCNHAGGAALGLVQPQSCL 397