Cucsa.120710.1 (mRNA) Cucumber (Gy14) v1

BLAST of Cucsa.120710.1 vs. Swiss-Prot
Match: NFM_BOVIN (Neurofilament medium polypeptide OS=Bos taurus GN=NEFM PE=1 SV=3)

HSP 1 Score: 97.4 bits (241), Expect = 3.3e-19
Identity = 98/350 (28.00%), Postives = 145/350 (41.43%), Query Frame = 1

Query: 7   PSRSVPNEKLEELVEASVREEEEEEDTLVILVVLDKEPVGNENPEG--EATNGEEEVKFW 66
           P ++   E  EE  E    E +EEE+        D+   G    EG  E   GE+E    
Sbjct: 514 PVKATAPELKEEEGEKEEEEGQEEEEEEEEAAKSDQAEEGGSEKEGSSEKEEGEQE---- 573

Query: 67  EELEERVLWNGE---VDKDDANKFEVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNR 126
           EE E      GE    +  +  K E +AE+  P+E+        KPE      VA  P  
Sbjct: 574 EEGETEAEGEGEEAAAEAKEEKKMEEKAEEVAPKEELAAEAKVEKPEKA-KSPVAKSPTT 633

Query: 127 ETPEPEAAAEAPNNPDPELAADADVPNNGALEVVAEDAPPNKDGADDAPADGAAPNKGAD 186
           ++P     A++P    PE A      +  A   VA+        A    A          
Sbjct: 634 KSP----TAKSPEAKSPE-AKSPTAKSPTAKSPVAKSPTAKSPEAKSPEAKSPTAKSPTA 693

Query: 187 EVPADEERAPKEGATELP---EEGAAPNEGAGKLPAPNKEVDEVPEEEAAPNKEAEEFAD 246
           + PA +  APK    E+    E GA   E   K+    KE  E P+EE A  KE +    
Sbjct: 694 KSPAAKSPAPKSPVEEVKPKAEAGAEKGEQKEKVEEEKKEAKESPKEEKAEKKEEK---- 753

Query: 247 EDEAPNPKELNPVEDEETPNKDEAEVAAEEGALEEDAPVEAEAREKEKGEEVEVEKEEEP 306
                 PK++   +  E+P K E+ V  E  A       E EA+E+EK +E E EKE+  
Sbjct: 754 ------PKDVPEKKKAESPVKAESPVKEEVPAKPVKVSPEKEAKEEEKPQEKEKEKEKVE 813

Query: 307 KPKEEEEGLDVGLGNGEEEEVGFE-----EEEEEDERDKGEGDDPKEKLV 344
           +   +EEG   GL    +E++        +EEE++ ++KG G + ++ +V
Sbjct: 814 EVGGKEEG---GLKESRKEDIAINGEVEGKEEEQETKEKGSGGEEEKGVV 840

HSP 2 Score: 75.9 bits (185), Expect = 1.0e-12
Identity = 84/331 (25.38%), Postives = 132/331 (39.88%), Query Frame = 1

Query: 41  DKEPVGNENPEGE----ATNGEEEVKFWEELEE-----RVLWNGEVDKDDANKFEVEAED 100
           ++E  G    EGE    A   +EE K  E+ EE      +    +V+K +  K  V    
Sbjct: 567 EQEEEGETEAEGEGEEAAAEAKEEKKMEEKAEEVAPKEELAAEAKVEKPEKAKSPVAKSP 626

Query: 101 -------KVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEPEAA-AEAPNNPDPELAAD 160
                  K PE  +P+   P    P     VA  P  ++PE ++  A++P    P   + 
Sbjct: 627 TTKSPTAKSPEAKSPEAKSPTAKSPTAKSPVAKSPTAKSPEAKSPEAKSPTAKSPTAKSP 686

Query: 161 ADVPNNGALEVVAEDAPPNKDGADDAPADGAAPNKGADEVPADEERAPKEGATELPEEGA 220
           A           A+   P     +  P   A   KG  +   +EE   K+ A E P+E  
Sbjct: 687 A-----------AKSPAPKSPVEEVKPKAEAGAEKGEQKEKVEEE---KKEAKESPKEEK 746

Query: 221 APNEGAGKLPAPNKEVDEVPEEEAAPNKE---AEEFADEDEAPNPKELNPVEDEETPNKD 280
           A  +       P K+  E P +  +P KE   A+      E    +E  P E E+   K 
Sbjct: 747 AEKKEEKPKDVPEKKKAESPVKAESPVKEEVPAKPVKVSPEKEAKEEEKPQEKEKEKEKV 806

Query: 281 EAEVAAEEGALEEDAPVEAEAREKEKGEEVEVEKEEEPKPKEEEEGL---DVGLGNGEEE 340
           E     EEG L+E    +     + +G+E E E +E+    EEE+G+    + +  G+E+
Sbjct: 807 EEVGGKEEGGLKESRKEDIAINGEVEGKEEEQETKEKGSGGEEEKGVVTNGLDVSPGDEK 866

Query: 341 EVGFEEEE-----EEDERDKGEGDDPKEKLV 344
           + G + EE     +  E+   EG D   K +
Sbjct: 867 KGGDKSEEKVVVTKMVEKITSEGGDGATKYI 883

HSP 3 Score: 72.4 bits (176), Expect = 1.1e-11
Identity = 58/163 (35.58%), Postives = 86/163 (52.76%), Query Frame = 1

Query: 209 AGKLPAPNKEVDEVPEE-----EAAPNKEAEEFADEDEAPNPKELNPVEDEETPNKDEAE 268
           A KL   +K V+E+ EE     E +  +EA     E+ A + KE   V++EE   K+E E
Sbjct: 444 APKLKVQHKFVEEIIEETKVEDEKSEMEEALTAITEELAVSVKE--EVKEEEAEEKEEKE 503

Query: 269 VAAEEGALEEDAPVEAEA---------REKEKGEEVEVEKEEEPKPKEEEEGLDVGLGNG 328
            A EE    + +PV+A A         +E+E+G+E E E+EE  K  + EEG     G+ 
Sbjct: 504 EAEEEVVAAKKSPVKATAPELKEEEGEKEEEEGQEEEEEEEEAAKSDQAEEGGSEKEGSS 563

Query: 329 EEEEVGFEEEEEEDERDKGEGDDPKEKLVDPKEKPVVEENGEE 358
           E+EE   E+EEE +   +GEG+   E   + KE+  +EE  EE
Sbjct: 564 EKEE--GEQEEEGETEAEGEGE---EAAAEAKEEKKMEEKAEE 599

HSP 4 Score: 60.1 bits (144), Expect = 5.8e-08
Identity = 84/348 (24.14%), Postives = 137/348 (39.37%), Query Frame = 1

Query: 7   PSRSVPNEKLEELVEASVREEEEEEDTLVILVVLDKEPVGNENPEGEATNGEEEVKFWEE 66
           P   V ++ +EE++E +  E+E+ E    +  + ++           A + +EEVK    
Sbjct: 445 PKLKVQHKFVEEIIEETKVEDEKSEMEEALTAITEEL----------AVSVKEEVK---- 504

Query: 67  LEERVLWNGEVDKDDANKFEVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEP 126
                              E EAE+K  +E+A + +   K  PV  +  A     E  E 
Sbjct: 505 -------------------EEEAEEKEEKEEAEEEVVAAKKSPV--KATAPELKEEEGEK 564

Query: 127 EAAAEAPNNPDPELAADADVPNNGALEVVAEDAPPNKDGADDAPADGAAPNKGADEVP-A 186
           E         + E AA +D    G  E   E +   ++G  +   +  A  +G +    A
Sbjct: 565 EEEEGQEEEEEEEEAAKSDQAEEGGSE--KEGSSEKEEGEQEEEGETEAEGEGEEAAAEA 624

Query: 187 DEERAPKEGATEL-PEEGAAPNEGAGKLPAPNKEVDEVPEEEAAPNKEAEEFADEDEAP- 246
            EE+  +E A E+ P+E  A      K       V + P  ++   K  E  + E ++P 
Sbjct: 625 KEEKKMEEKAEEVAPKEELAAEAKVEKPEKAKSPVAKSPTTKSPTAKSPEAKSPEAKSPT 684

Query: 247 --NPKELNPVEDEETPNKDEAEVAAEEGALEEDAPVEAEAREKEKGEEVEVEKEEEPKPK 306
             +P   +PV    T    EA+ + E  +    +P       K    +  VE   E KPK
Sbjct: 685 AKSPTAKSPVAKSPTAKSPEAK-SPEAKSPTAKSPTAKSPAAKSPAPKSPVE---EVKPK 744

Query: 307 EEEEGLDVGLGNGEEEEVGFEEEEEEDERDKGEGDDPKEKLVDPKEKP 350
            E      G   GE++E   EE++E  E  K E  + KE+   PK+ P
Sbjct: 745 AE-----AGAEKGEQKEKVEEEKKEAKESPKEEKAEKKEE--KPKDVP 744

BLAST of Cucsa.120710.1 vs. Swiss-Prot
Match: RTL1_MOUSE (Retrotransposon-like protein 1 OS=Mus musculus GN=Rtl1 PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 7.6e-16
Identity = 59/143 (41.26%), Postives = 81/143 (56.64%), Query Frame = 1

Query: 216  NKEVDEVPEEEAAPNKEAEEFADEDEAPNPKELNPVEDEETPNKDEAEVAAEEGALEEDA 275
            +KE DE  EEE    +E EE  D +E    +E +  E+EE    DE E    EG  EED 
Sbjct: 1292 DKEDDEEEEEEDGEEEEGEEEEDGEEEEGEEEEDGEEEEEEEEDDEEE----EGEEEEDG 1351

Query: 276  PVEAEAREKEKGEEVEVEKEEEPKPKEEEEGLDVGLGNGEEEEVGFEEEEEEDERDKGEG 335
              E E  E+E GEE E E+EE+    EEEEG +     GEEEE G EEEEEE++ ++ E 
Sbjct: 1352 E-EEEGEEEEDGEEEEGEEEED---GEEEEGEE----EGEEEEEGEEEEEEEEDEEEEEE 1411

Query: 336  DDPKEKLVDPKEKPVVEENGEED 359
            ++ +E+  + +E+   EE  EE+
Sbjct: 1412 EEEEEEEEEEEEEEEEEEEEEEE 1422

HSP 2 Score: 40.8 bits (94), Expect = 3.7e-02
Identity = 43/163 (26.38%), Postives = 61/163 (37.42%), Query Frame = 1

Query: 26   EEEEEEDTLVILVVLDKEPVGNENPEGEATNGEEEVKFWEELEERVLWNGEVDKDDANKF 85
            EEEEEED         +E  G E  +GE   GEEE    EE EE         ++D  + 
Sbjct: 1297 EEEEEED--------GEEEEGEEEEDGEEEEGEEEEDGEEEEEE---------EEDDEEE 1356

Query: 86   EVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEPEAAAEAPNNPDPELAADAD 145
            E E E+   EE+  +  D  + E    ED       E  E E   E     + E   + D
Sbjct: 1357 EGEEEEDGEEEEGEEEEDGEEEEGEEEED------GEEEEGEEEGEEEEEGEEEEEEEED 1416

Query: 146  VPNNGALEVVAEDAPPNKDGADDAPADGAAPNKGADEVPADEE 189
                   E   E+    ++  ++   +     +  DE   DEE
Sbjct: 1417 EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEDEEEEDEEEEDEE 1436

HSP 3 Score: 40.4 bits (93), Expect = 4.8e-02
Identity = 39/129 (30.23%), Postives = 56/129 (43.41%), Query Frame = 1

Query: 14   EKLEELVEASVREEEEEEDTLVILVVLDKEPVGNENPEGEATNGEEEVKFWEELEERVLW 73
            E+ EE  +    E EEEED         +E  G E  +GE   GEEE +  EE EE    
Sbjct: 1339 EEGEEEEDGEEEEGEEEED--------GEEEEGEEEEDGEEEEGEEEGEEEEEGEEE--- 1398

Query: 74   NGEVDKDDANKFEVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEPEAAAEAP 133
              E +++D  + E E E++  EE+  +  +  + E    E+       E  E E   E P
Sbjct: 1399 --EEEEEDEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEDEE-------EEDEEEEDEEVP 1447

Query: 134  NNPDPELAA 143
            +     LAA
Sbjct: 1459 SMVRELLAA 1447

HSP 4 Score: 35.0 bits (79), Expect = 2.0e+00
Identity = 22/71 (30.99%), Postives = 37/71 (52.11%), Query Frame = 1

Query: 229 PNKEAEEFADE---DEAPNPKELNPVEDEETPNKDEAEVAAEEGALEEDAPVEAEAREKE 288
           P +  +E +D+   D + +  E  P E ++  + D++ V  E GA E   PV A  +EK 
Sbjct: 822 PREADDETSDQPSSDGSDDLSESEPSELQQAGDSDQSGVFYESGARETLEPVSARMQEKA 881

Query: 289 KGEEVEVEKEE 297
           + +E   E+EE
Sbjct: 882 RQQEKAREQEE 892

BLAST of Cucsa.120710.1 vs. Swiss-Prot
Match: ICP22_SHV21 (Transcriptional regulator ICP22 homolog OS=Saimiriine herpesvirus 2 (strain 11) GN=73 PE=3 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.7e-15
Identity = 62/177 (35.03%), Postives = 85/177 (48.02%), Query Frame = 1

Query: 181 DEVPADEERAPKEGATELPEEGAAPNEGAGKLPAPNKEVDEVPEEEAAPNKEAEEFADED 240
           +EV  + E   + G  E   EG    EG  +      E  E  EEEA   +EAEE A+E+
Sbjct: 65  EEVEEEGEERERRGEEEREGEGGEEGEGREEAEEEEAEEKEAEEEEA---EEAEEEAEEE 124

Query: 241 EAPNPKELNPVEDEETPNKDEAEVAAEEGALEEDAPVEAEAREKEKGEEVEVEKEEEPKP 300
           EA   +      +EE   ++EAE A EE A  E+A  EAE  E E+  E E E+ EE + 
Sbjct: 125 EAEEAEAEEEEAEEEEAEEEEAEEAEEEEA--EEAEEEAEEEEAEEEAEEEAEEAEEAEE 184

Query: 301 KEEEEGLDVGLGNGEEEEVGFEEEEEEDERDKGEGDDPKEKLVDPKEKPVVEENGEE 358
           + EEE  +       EE    EEE EE E +  E ++  E+  + +E    EE  EE
Sbjct: 185 EAEEEAEEAEEAEEAEEA---EEEAEEAEEEAEEAEEEAEEAEEAEEAEEAEEEAEE 233

HSP 2 Score: 81.3 bits (199), Expect = 2.4e-14
Identity = 72/214 (33.64%), Postives = 95/214 (44.39%), Query Frame = 1

Query: 153 EVVAEDAPPNKDGADDAPADGAAPNKGADEVPADE-----------ERAPKEGATELPEE 212
           EV  E     + G ++   +G    +G +E   +E           E A +E   E  EE
Sbjct: 66  EVEEEGEERERRGEEEREGEGGEEGEGREEAEEEEAEEKEAEEEEAEEAEEEAEEEEAEE 125

Query: 213 GAAPNEGAGKLPAPNKEVDEVPEEEAAPNKEAEEFADEDEAPNPKELNPVEDEETPNKDE 272
             A  E A +  A  +E +E  EEEA   +EAEE A+E+EA    E      EE    +E
Sbjct: 126 AEAEEEEAEEEEAEEEEAEEAEEEEA---EEAEEEAEEEEAEEEAE------EEAEEAEE 185

Query: 273 AEVAAEEGALEEDAPVEAEAREKEKGEEVEVEKEEEPKPKEEEEGLDVGLGNGEEEEVGF 332
           AE  AEE A E +   EAE  E E+ EE E E EE  +  EE E  +      E EE   
Sbjct: 186 AEEEAEEEAEEAEEAEEAEEAE-EEAEEAEEEAEEAEEEAEEAEEAEEA---EEAEEEAE 245

Query: 333 EEEEEEDERDKGEGDDPKEKLVDPKEKPVVEENG 356
           E EEEE+E        P  K+V   +KP  +  G
Sbjct: 246 EAEEEEEEAGPSTPRLPHYKVVG--QKPSTQPGG 264

HSP 3 Score: 77.8 bits (190), Expect = 2.7e-13
Identity = 65/199 (32.66%), Postives = 87/199 (43.72%), Query Frame = 1

Query: 160 PPNKDGADDAPADGAAPNKGADEVPADEERAPKEGATELPEEGAAPNEGAGKLPAPNKEV 219
           P   D  +      AA  +       +EE   +E   E   EG    EG G+  A  +E 
Sbjct: 42  PQGDDDINTTHQQQAALTEEQRREEVEEEGEERERRGEEEREGEGGEEGEGREEAEEEEA 101

Query: 220 DEVPEEEAAPNKEAEEFADEDEAPNPK-ELNPVEDEETPNKDEAEVAAEEGALEEDAPVE 279
           +E   EE    +EAEE   E+EA   + E    E+EE   ++  E  AEE   EE    E
Sbjct: 102 EEKEAEE----EEAEEA--EEEAEEEEAEEAEAEEEEAEEEEAEEEEAEEAEEEEAEEAE 161

Query: 280 AEAREKEKGEEVEVEKEEEPKPKEEEEGLDVGLGNGEEEEVGFEEEEEEDERDKGEGDDP 339
            EA E+E  EE E E EE  + +EE E         EE E   EEE EE E +  E ++ 
Sbjct: 162 EEAEEEEAEEEAEEEAEEAEEAEEEAEEEAEEAEEAEEAEEA-EEEAEEAEEEAEEAEEE 221

Query: 340 KEKLVDPKEKPVVEENGEE 358
            E+  + +E    EE  EE
Sbjct: 222 AEEAEEAEEAEEAEEEAEE 233

HSP 4 Score: 73.9 bits (180), Expect = 3.9e-12
Identity = 59/180 (32.78%), Postives = 80/180 (44.44%), Query Frame = 1

Query: 127 EAAAEAPNNPDPELAADADVPNNGALEVVAEDAPPNKDGADDAP-ADGAAPNKGADEVPA 186
           E   E     + E   +      G  E   E+A   +   ++A  A+  A  + A+E  A
Sbjct: 69  EEGEERERRGEEEREGEGGEEGEGREEAEEEEAEEKEAEEEEAEEAEEEAEEEEAEEAEA 128

Query: 187 DEERAPKEGATELPEEGAAPNEGAGKLPAPNKEVDEVPEEEAAPNKEAEEFADEDEAPNP 246
           +EE A +E A E   E A   E         +E +E  EEE A  +  EE  + +EA   
Sbjct: 129 EEEEAEEEEAEEEEAEEAEEEEA--------EEAEEEAEEEEAEEEAEEEAEEAEEAEEE 188

Query: 247 KELNPVEDEETPNKDEAEVAAEEGALEEDAPVEAEAREKEKGEEVEVEKEEEPKPKEEEE 306
            E    E EE    +EAE  AEE A EE    E EA E E+ EE E  +EE  + +EEEE
Sbjct: 189 AEEEAEEAEEAEEAEEAEEEAEE-AEEEAEEAEEEAEEAEEAEEAEEAEEEAEEAEEEEE 239

HSP 5 Score: 52.4 bits (124), Expect = 1.2e-05
Identity = 52/201 (25.87%), Postives = 81/201 (40.30%), Query Frame = 1

Query: 14  EKLEELVEASVREEEEEEDTLVILVVLDKEPVGNENPEGEATNGEEEVKFWEELEERVLW 73
           E++EE  E   R  EEE           +E  G E  EG     EEE +  +E EE    
Sbjct: 65  EEVEEEGEERERRGEEE-----------REGEGGEEGEGREEAEEEEAE-EKEAEEEEAE 124

Query: 74  NGEVDKDDANKFEVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEPEAAAEAP 133
             E + ++    E EAE++  EE+  +  +  + E    E+ A     E  E EA  EA 
Sbjct: 125 EAEEEAEEEEAEEAEAEEEEAEEEEAEEEEAEEAE----EEEAEEAEEEAEEEEAEEEAE 184

Query: 134 NNPDPELAADADVPNNGALEVVAEDAPPNKDGADDAPADGAAPNKGADEV-PADEERAPK 193
              +    A+ +          AE+A   ++ A++A  +     + A+E   A+E    +
Sbjct: 185 EEAEEAEEAEEEAEEEAEEAEEAEEAEEAEEEAEEAEEEAEEAEEEAEEAEEAEEAEEAE 244

Query: 194 EGATELPEEGAAPNEGAGKLP 214
           E A E  EE         +LP
Sbjct: 245 EEAEEAEEEEEEAGPSTPRLP 249

HSP 6 Score: 50.4 bits (119), Expect = 4.6e-05
Identity = 62/253 (24.51%), Postives = 97/253 (38.34%), Query Frame = 1

Query: 14  EKLEELVEASVREEEEEEDTLVILVVLDKEPVGNENPEGEATNGEEEVKFWEELEERVLW 73
           E  EE  E    E+E EE+        + E    E  E EA   E E +  EE E     
Sbjct: 91  EGREEAEEEEAEEKEAEEE--------EAEEAEEEAEEEEAEEAEAEEEEAEEEEAEEEE 150

Query: 74  NGEVDKDDANKFEVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEPEAAAEAP 133
             E ++++A + E EAE++  EE+A +  +                  E  E EA  EA 
Sbjct: 151 AEEAEEEEAEEAEEEAEEEEAEEEAEEEAE----------------EAEEAEEEAEEEAE 210

Query: 134 NNPDPELAADADVPNNGALEVVAEDAPPNKDGADDAPADGAAPNKGADEVPADEERAPKE 193
              + E A +A+     A E  AE+A    + A++A     A  +  +    +EE  P  
Sbjct: 211 EAEEAEEAEEAEEEAEEA-EEEAEEAEEEAEEAEEAEEAEEAEEEAEEAEEEEEEAGPST 270

Query: 194 ---------GATELPEEGAAPN-------EGAGKLPAPNKEVDEVPEEEAAPNKEAEEFA 251
                    G     + G  P        +   +LP   +  D+VP++  A NK   +  
Sbjct: 271 PRLPHYKVVGQKPSTQPGGVPKLCLKMQPQHRSRLPKGKQSHDKVPKKYQARNKFFSQ-- 313

BLAST of Cucsa.120710.1 vs. Swiss-Prot
Match: NFH_HUMAN (Neurofilament heavy polypeptide OS=Homo sapiens GN=NEFH PE=1 SV=4)

HSP 1 Score: 84.0 bits (206), Expect = 3.8e-15
Identity = 92/357 (25.77%), Postives = 148/357 (41.46%), Query Frame = 1

Query: 8   SRSVPNEKLEELVEASVREEEEEEDTLVILVVLDKEPVGNENPEGEATNGEEEVKFWEEL 67
           ++S   E+ +   EA    +EE +    +     K P   ++P  E     E+ K  E+ 
Sbjct: 604 AKSPVKEEAKSPAEAKSPVKEEAKSPAEV-----KSPEKAKSPTKEEAKSPEKAKSPEKA 663

Query: 68  EERVLWNGEVDKDDANKFEVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEPE 127
           +       E    +  K  V+AE K PE    K   P K E   PE        E   PE
Sbjct: 664 KSPE--KEEAKSPEKAKSPVKAEAKSPE----KAKSPVKAEAKSPEKAKSPVKEEAKSPE 723

Query: 128 AA-------AEAPNNPDPELAADADVPNNGALEVVAEDAPPNKDGADDAPADGAAPNKGA 187
            A       A++P      +  +A  P      V  E   P K  + +        +  A
Sbjct: 724 KAKSPVKEEAKSPEKAKSPVKEEAKTPEKAKSPVKEEAKSPEKAKSPEKAKTLDVKSPEA 783

Query: 188 DEVPADEERAPKEGATELPEEGAAPNEGAGKLPAPNKEVDEVPEEEAAPNKEAEEFADED 247
                +E R+P   A + PE          K  +P KE  + PE+  +P KE  + A E 
Sbjct: 784 KTPAKEEARSP---ADKFPE----------KAKSPVKEEVKSPEKAKSPLKEDAK-APEK 843

Query: 248 EAPNPKEL-NPVEDEETPNKDEAEVAAEEGALEEDAPVEAEAREKEKGEEVEVEKEEEPK 307
           E P  +E+ +PV++EE P + + +   ++ A EE AP   +  EK+  ++ E  K+E PK
Sbjct: 844 EIPKKEEVKSPVKEEEKPQEVKVKEPPKK-AEEEKAPATPKTEEKKDSKKEEAPKKEAPK 903

Query: 308 PKEEEEGLDVGLGNGEEEEVGFEEEEEEDERDKGEGDDPKEKLVDPKEKPVVEENGE 357
           PK EE+  +  +   +E +V  ++EE ED++     +      V+ KE    +E  E
Sbjct: 904 PKVEEK-KEPAVEKPKESKVEAKKEEAEDKKKVPTPEKEAPAKVEVKEDAKPKEKTE 933

HSP 2 Score: 76.6 bits (187), Expect = 6.0e-13
Identity = 95/365 (26.03%), Postives = 155/365 (42.47%), Query Frame = 1

Query: 11  VPNEKLEELVEASVRE----EEEEEDTLVILVVLDKEPVGNENPEGEATNGEEEVKFWEE 70
           V +E+  ++VE S +E    EE+ E+T V   V ++E    +  EG+   G EE    EE
Sbjct: 438 VKSEEKIKVVEKSEKETVIVEEQTEETQVTEEVTEEEEKEAKEEEGKEEEGGEE----EE 497

Query: 71  LEERVLWNGEVDKDDANKFEVEAEDKVPEE--DAPKGLDPNKPEPVLPEDVAGVPNRETP 130
            E           ++A   E EA+  V EE     +   P K E   P +V      ++P
Sbjct: 498 AEGGEEETKSPPAEEAASPEKEAKSPVKEEAKSPAEAKSPEKEEAKSPAEVKSPEKAKSP 557

Query: 131 EPEAAAEAPNNPDPELAADADVPNNGALEVVAEDAPPNKDGADDAPADGAAPNKGADEVP 190
             E A   P    PE   +A  P        AE   P K           +P K   + P
Sbjct: 558 AKEEAKSPPEAKSPE-KEEAKSP--------AEVKSPEK---------AKSPAKEEAKSP 617

Query: 191 ADEERAPKEGATELPEEGAAPNEGAGKLPAPNKEVDEVPEEEAAPNKEAEEFADEDEAPN 250
           A E ++P++  + + EE  +P E      +P KE  + P E  +P K       ++EA +
Sbjct: 618 A-EAKSPEKAKSPVKEEAKSPAEAK----SPVKEEAKSPAEVKSPEKAKSP--TKEEAKS 677

Query: 251 PKELNPVEDEETPNKDEAE--------VAAEEGALEE-DAPVEAEAREKEKGEEVEVEKE 310
           P++    E  ++P K+EA+        V AE  + E+  +PV+AEA+  EK +     KE
Sbjct: 678 PEKAKSPEKAKSPEKEEAKSPEKAKSPVKAEAKSPEKAKSPVKAEAKSPEKAK--SPVKE 737

Query: 311 EEPKPKEEEEGLDVGLGNGEEEEVGFEEEEEEDERDKG---EGDDPKEKLVDPKEKPVVE 358
           E   P++ +  +     + E+ +   +EE +  E+ K    E     EK   P++   ++
Sbjct: 738 EAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKTPEKAKSPVKEEAKSPEKAKSPEKAKTLD 771

HSP 3 Score: 68.6 bits (166), Expect = 1.6e-10
Identity = 88/325 (27.08%), Postives = 139/325 (42.77%), Query Frame = 1

Query: 42   KEPVGNE--NPEGEATNGEEEVKFWEELEERVLWNGEVDKDDANKFEVEAEDKVPEE--- 101
            K PV  E  +PE   +  +EE K  E+ +  V    E    +  K  V+ E K PE+   
Sbjct: 701  KSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPV--KEEAKTPEKAKSPVKEEAKSPEKAKS 760

Query: 102  -DAPKGLDPNKPEPVLP-EDVAGVPNRETPEPEAA-----AEAPNNPDPELAADADVPNN 161
             +  K LD   PE   P ++ A  P  + PE   +      ++P      L  DA  P  
Sbjct: 761  PEKAKTLDVKSPEAKTPAKEEARSPADKFPEKAKSPVKEEVKSPEKAKSPLKEDAKAP-- 820

Query: 162  GALEVVAEDAPPNKDGADDAPADGAAPNKGADEVP---ADEERAPKEGATELPEEGAAPN 221
                   E   P K+       +   P +   + P   A+EE+AP    TE  E+  +  
Sbjct: 821  -------EKEIPKKEEVKSPVKEEEKPQEVKVKEPPKKAEEEKAPATPKTE--EKKDSKK 880

Query: 222  EGAGKLPAPNKEVDEVPEEEAAPNKEAEEFADEDEAPNPKELNPVEDEETPNKDEAEVAA 281
            E A K  AP  +V+E  E      KE++  A ++EA + K++ P  ++E P K E +  A
Sbjct: 881  EEAPKKEAPKPKVEEKKEPAVEKPKESKVEAKKEEAEDKKKV-PTPEKEAPAKVEVKEDA 940

Query: 282  ---EEGALEEDAPVEAEAREKEKGEEVEVEKEEEPKPKEEEEGLDVGLGNGEEEEVGFEE 341
               E+  + +  P +A+A+E  K  E   +KE  P+ K+ +E          EE+   E 
Sbjct: 941  KPKEKTEVAKKEPDDAKAKEPSKPAE---KKEAAPEKKDTKE----EKAKKPEEKPKTEA 1000

Query: 342  EEEEDERDKGEGDDPKEKLVDPKEK 349
            + +ED  DK    +P +   +  EK
Sbjct: 1001 KAKED--DKTLSKEPSKPKAEKAEK 1002

HSP 4 Score: 37.7 bits (86), Expect = 3.1e-01
Identity = 43/151 (28.48%), Postives = 65/151 (43.05%), Query Frame = 1

Query: 207 EGAGKLPAPNKEVDEVPEEEAAPNKEAEEFADEDEAPNPKELNPVEDEETPNKDEAEVAA 266
           EG  K+P+ +  +    EE+    +++E           KE   VE++     +E +V  
Sbjct: 424 EGLPKIPSVSTHIKVKSEEKIKVVEKSE-----------KETVIVEEQT----EETQVTE 483

Query: 267 EEGALEEDAPVEAEAREKEKGEEVEVE-KEEEPK--PKEEEEGLDVGLGNGEEEEVGFEE 326
           E    EE    E E +E+E GEE E E  EEE K  P EE    +    +  +EE     
Sbjct: 484 EVTEEEEKEAKEEEGKEEEGGEEEEAEGGEEETKSPPAEEAASPEKEAKSPVKEEAKSPA 543

Query: 327 EEEEDERDKGEGDDPKE-KLVDPKEKPVVEE 354
           E +  E++  E   P E K  +  + P  EE
Sbjct: 544 EAKSPEKE--EAKSPAEVKSPEKAKSPAKEE 557

BLAST of Cucsa.120710.1 vs. Swiss-Prot
Match: NUCL_MESAU (Nucleolin OS=Mesocricetus auratus GN=NCL PE=1 SV=2)

HSP 1 Score: 82.0 bits (201), Expect = 1.4e-14
Identity = 74/264 (28.03%), Postives = 114/264 (43.18%), Query Frame = 1

Query: 119 PNRETPEPEAAAEAPNNPDPELAADADVPNNGALEVVAEDAPP-----NKDGADDAPADG 178
           P +E  E     E     D     +  +P     +  A  A        K  A   PA  
Sbjct: 20  PPKEVEEDSEDEEMSEEEDDSSGEEVVIPQKKGKKATATPAKKVVVSQTKKVAVPTPAKK 79

Query: 179 AA--PNKGADEVPADEERAPKEGATELPEEGAAPNEGAGKLPAPNKEVDEVPEEEAAPNK 238
           AA  P K A   PA +   P +      ++GA   +    +  P K+    P + A   K
Sbjct: 80  AAVTPGKKAAATPAKKAVTPAKAVATPGKKGAT--QAKALVATPGKKGAVTPAKGAKNGK 139

Query: 239 EAE-EFADEDEAPNPKELNPVEDEETPNKDEAE---VAAEEGALEEDAPVEAEAREKEKG 298
            A+ E +DEDE  +  E +  EDEE   +DE E   V  ++G +   AP  +E  ++E+ 
Sbjct: 140 NAKKEDSDEDEDDDDDEDDSDEDEEDEEEDEFEPPVVKGKQGKVAAAAPA-SEDEDEEED 199

Query: 299 EEVEVEKEEEPKPKEEEEGLDVGLGNGE---------------EEEVGFEEEEEEDERDK 357
           EE E E EEE    EEEE +++    G+               EE+   EEE+E++E D+
Sbjct: 200 EEEEEEDEEEEDDSEEEEAMEITPAKGKKAPAKVVPVKAKNVAEEDDDDEEEDEDEEEDE 259

HSP 2 Score: 70.5 bits (171), Expect = 4.3e-11
Identity = 79/292 (27.05%), Postives = 122/292 (41.78%), Query Frame = 1

Query: 76  EVDKDDANKFEVEAEDK-------VPEEDAPKGL-DPNKPEPVLPEDVAGVPNRE----- 135
           EV++D  ++   E ED        +P++   K    P K   V       VP        
Sbjct: 23  EVEEDSEDEEMSEEEDDSSGEEVVIPQKKGKKATATPAKKVVVSQTKKVAVPTPAKKAAV 82

Query: 136 TPEPEAAAEAPNNPDPELAADADVPNNGALEVVAEDAPPNKDGADDAPADGAAPNKGADE 195
           TP  +AAA           A A     GA +  A  A P K GA   PA GA   K A +
Sbjct: 83  TPGKKAAATPAKKAVTPAKAVATPGKKGATQAKALVATPGKKGAV-TPAKGAKNGKNAKK 142

Query: 196 VPADE--------------ERAPKEGATELPEEGAAPNEGAGKLPAPNKEVDEVPEEEAA 255
             +DE              E   +E   E P       + A   PA   E +E  EEE  
Sbjct: 143 EDSDEDEDDDDDEDDSDEDEEDEEEDEFEPPVVKGKQGKVAAAAPASEDEDEEEDEEEEE 202

Query: 256 PNKEAEEFADEDEAPNPKELNPVEDEETPNKD---EAEVAAEEGALEEDAPVEAEAREKE 315
            ++E E+ ++E+EA    E+ P + ++ P K    +A+  AEE   +E+   + E  E+E
Sbjct: 203 EDEEEEDDSEEEEA---MEITPAKGKKAPAKVVPVKAKNVAEEDDDDEEEDEDEEEDEEE 262

Query: 316 KGEEVEVEKEEEPKPKEEEEGLDVGLGNGEEEEVGFEEEEEEDERDKGEGDD 338
           + +E E E+EEE +P +   G         ++E+  ++E  E ++ K EG +
Sbjct: 263 EEDEEEEEEEEEEEPVKPAPG-------KRKKEMTKQKEVPEAKKQKVEGSE 303

BLAST of Cucsa.120710.1 vs. TrEMBL
Match: A0A0A0KDT9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G106740 PE=4 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 8.1e-41
Identity = 95/120 (79.17%), Postives = 102/120 (85.00%), Query Frame = 1

Query: 236 FADEDEAPNPKELNPVEDEETPNKDEAEVAAEEGALEEDAPVEAEAREKEKG---EEVEV 295
           FADE+EA N KELNPVEDEET NKDEA VA EEG LE+DA VEAEAREKE G   EEVE 
Sbjct: 15  FADENEALNAKELNPVEDEETLNKDEAGVAEEEGVLEKDALVEAEAREKENGEKVEEVEE 74

Query: 296 EKEEEPKPKEEEEGLDVGLGNGEEEEVGF---EEEEEEDERDKGEGDDPKEKLVDPKEKP 350
           EKE+EPKPK EEEGL+VGLGNGE+EEVGF   EEEEEEDERDKG+GDDPKEK+ DPK KP
Sbjct: 75  EKEKEPKPK-EEEGLEVGLGNGEDEEVGFEEEEEEEEEDERDKGKGDDPKEKVEDPKPKP 133

BLAST of Cucsa.120710.1 vs. TrEMBL
Match: A0A0A0KDT9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G106740 PE=4 SV=1)

HSP 1 Score: 38.5 bits (88), Expect = 2.0e+01
Identity = 34/99 (34.34%), Postives = 50/99 (50.51%), Query Frame = 1

Query: 216 NKEVDEVPEEEAAPNKEAEEFADEDEAPNPKELNPVEDEET----PNKDEA-EVAA---- 275
           NK+   V EEE    K+A   A+  E  N +++  VE+E+     P ++E  EV      
Sbjct: 37  NKDEAGVAEEEGVLEKDALVEAEAREKENGEKVEEVEEEKEKEPKPKEEEGLEVGLGNGE 96

Query: 276 -EEGALEEDAPVEAEARE-KEKGEEVEVEKEEEPKPKEE 304
            EE   EE+   E E    K KG++ + EK E+PKPK +
Sbjct: 97  DEEVGFEEEEEEEEEDERDKGKGDDPK-EKVEDPKPKPD 134

HSP 2 Score: 124.4 bits (311), Expect = 2.8e-25
Identity = 111/366 (30.33%), Postives = 173/366 (47.27%), Query Frame = 1

Query: 14  EKLEELVEASVREEEEE---------EDTLVILVVLDKEPVGNENPEGEATNGEEEVKFW 73
           E++EE+VE  V EEEEE         E+   + V +++E    E  E E    EEE +  
Sbjct: 236 EEVEEVVEIEVEEEEEEVEEVEVEVEEEEEEMEVEVEEEEEEEEEVEEEVVEEEEEQEEE 295

Query: 74  EELEERVLWNGEVDKDDANKFEVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETP 133
           EE+EE  +  GE ++++  K E E E++V  E+  + ++  + E V+ E+       E  
Sbjct: 296 EEVEEVEVEVGEEEEEEEVK-EEEVEEEVEVEEEEEEVEEVEEEEVVEEEEEQEEEEEVE 355

Query: 134 EPEAAAEAPNNPDPELAADADVPNNGALEVVAEDAPPNKD--GADDAPADGAAPNKGADE 193
           E E   E  +  +     + +V      EV  E+    ++    ++   +        +E
Sbjct: 356 EEEEEEEVEDEVEEIEVEEEEVEEEEEEEVEVEEEEEEEEVVEVEEEEEEDVVVEVEEEE 415

Query: 194 VPADEERAPKEGATELPEEGAAPNEGAGKLPAPNKEVDEVPEEEAAPNKEAEEFADEDEA 253
           V  +EE    E   E  EE     E   +     +E ++  EEE    +E EE  DE E 
Sbjct: 416 VEVEEEVVEVEEEEEEDEEEEEEVEEVEEEEVVEEEEEQEEEEEVEEEEEEEEVEDEVEE 475

Query: 254 PNPKELNPVEDEETPNKDEAEVAAEEGALEEDAPVEAEAREKEKGEE---VEVEKEEEPK 313
                    E+EE    +E EV  EE  +EE+   E E  E+E+ EE   VEVE+EEE +
Sbjct: 476 EE-------EEEEVEEVEEIEVEEEEEEVEEEEEEEVEVEEEEEEEEEEVVEVEEEEEEE 535

Query: 314 PKEEEEGLDVGLGNG----EEEEVGFEEEEEEDE-RDKG--EGDDPKEKLVDPKEKPVVE 359
            +EEEE + V +       EEE V  EEEEEE+E  ++G  EG+D +EK ++ K +  V+
Sbjct: 536 EEEEEEDVVVEVEEEEVEVEEEVVEVEEEEEEEEVEEEGEVEGEDEEEKEMEEKVEEKVK 593

BLAST of Cucsa.120710.1 vs. TrEMBL
Match: A0A072VM56_MEDTR (Uncharacterized protein OS=Medicago truncatula GN=MTR_2g090985 PE=4 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 8.4e-22
Identity = 114/362 (31.49%), Postives = 166/362 (45.86%), Query Frame = 1

Query: 14  EKLEELVEASVREEEEEEDTLVILV-----VLDKEPVGNENPEGEATNGEEEVKFWEELE 73
           E+  E VE  V EEEEEE+     V     V ++E    E  E E    EE+V   EE+E
Sbjct: 138 EEEVEKVEVEVGEEEEEEEVKEEEVEEEVEVEEEEEEVEEVEEIEVEEEEEKV---EEVE 197

Query: 74  ERVLWNGEVDKDDAN-KFEVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEPE 133
           E V+   E  +D+   + E E E++V EE+  + ++  + E  + E V      E  E E
Sbjct: 198 EEVVEEEEEQEDEEEVEEEEEEEEEVEEEEEEEEVELEEEEEEVEEVVEIEVEEEEEEVE 257

Query: 134 AAAEAPNNPDPELAADADVPNNGALEVVAEDAPPNKDGADDAPADGAAPNKGADEVPADE 193
                    + E+  + +       EV  E     ++  ++   +      G +E   +E
Sbjct: 258 EVEVEVEEEEEEMEVEVEEEEEEEEEVEEEVVEEEEEQEEEEEVEEVEVEVGEEE---EE 317

Query: 194 ERAPKEGATELPEEGAAPNEGAGKLPAPNKEVDEVPEEEAAPNKEAEEFADEDEAPNPKE 253
           E   +E   E+ EE     E         +EV E  EEE    +E EE  +E+E  +  E
Sbjct: 318 EEVKEE---EVEEEVEVEEEEEEVEEVEEEEVVE-EEEEQEEEEEVEEEEEEEEVEDEVE 377

Query: 254 LNPVEDEETPNKDEAEVAAEEGALEE-----------DAPVEAEAREKEKGEE-VEVEKE 313
              VE+EE   ++E EV  EE   EE           D  VE E  E E  EE VEVE+E
Sbjct: 378 EIEVEEEEVEEEEEEEVEVEEEEEEEEVVEVEEEEEEDVVVEVEEEEVEVEEEVVEVEEE 437

Query: 314 EEPKPKEEEEGLDVGLGNGEEEEVGFEEEEEEDERDKGEGDDPKEKLVDPKEKPVVEENG 358
           EE   +EEEE  +V     EEEEV  EEEEE++E ++ E ++ +E++ D  E+   EE  
Sbjct: 438 EEEDEEEEEEVEEV-----EEEEV-VEEEEEQEEEEEVEEEEEEEEVEDEVEEEEEEEEV 483

HSP 2 Score: 108.2 bits (269), Expect = 2.1e-20
Identity = 106/345 (30.72%), Postives = 155/345 (44.93%), Query Frame = 1

Query: 14  EKLEELVEASVREEEEEEDTLVILVVLDKEPVGNENPEGEATNGEEEVKFWEELEERVLW 73
           E+ EE  E  V EEE EE+     V L++E V  E  E E    EEE +  EE EE +  
Sbjct: 52  EEEEEEEEEEVEEEEVEEE-----VELEEEEV--EEVEVEEIEVEEEEEEVEEEEEEMEV 111

Query: 74  NGEVDKDDANKFEVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEPEAAAEAP 133
             EV+ ++  + EVE E++V EE   +     + E V           E  E E   E  
Sbjct: 112 EVEVEVEEEEEEEVEVEEEVEEEVVEEEEKQEEEEEV-----------EKVEVEVGEEEE 171

Query: 134 NNPDPELAADADVPNNGALEVVAEDAPPNKDGADDAPADGAAPNKGADEVPADEERAPKE 193
                E   + +V      E V E                       +E+  +EE    E
Sbjct: 172 EEEVKEEEVEEEVEVEEEEEEVEE----------------------VEEIEVEEEEEKVE 231

Query: 194 GATELPEEGAAPNEGAGKLPAPNKEVDEVPEEEAAPNKEAEEFADEDEAPNPKELNPVED 253
              E   E     E   ++    +E +EV EEE     E EE  +E+E     E+   E+
Sbjct: 232 EVEEEVVEEEEEQEDEEEVEEEEEEEEEVEEEEEEEEVELEE--EEEEVEEVVEIEVEEE 291

Query: 254 EETPNKDEAEVAAEEGALEEDAPVEAEAREKEKGEEVEVEKEEEPKPKEEEEGLDVGLGN 313
           EE   + E EV  EE  +E +  VE E  E+E+ EE  VE+EEE + +EE E ++V +G 
Sbjct: 292 EEEVEEVEVEVEEEEEEMEVE--VEEEEEEEEEVEEEVVEEEEEQEEEEEVEEVEVEVGE 348

Query: 314 GEEEEVGFEEEEEEDERDKGEGDDPKEKLVDPKEKPVVEENGEED 359
            EEEE   EEE EE+     E ++ +E++ + +E+ VVEE  E++
Sbjct: 352 EEEEEEVKEEEVEEEV----EVEEEEEEVEEVEEEEVVEEEEEQE 348

HSP 3 Score: 102.1 bits (253), Expect = 1.5e-18
Identity = 107/366 (29.23%), Postives = 171/366 (46.72%), Query Frame = 1

Query: 11  VPNEKLEEL-VEASVREEEEEEDTLVILVVLDKEPVGNENPEGEATNGEEEVKFWEELEE 70
           V   ++EE+ VE    E EEEE+ + + V ++ E    E  E E    EE V+  E+ EE
Sbjct: 78  VEEVEVEEIEVEEEEEEVEEEEEEMEVEVEVEVEEEEEEEVEVEEEVEEEVVEEEEKQEE 137

Query: 71  RVLWNGEVDKDDANKFEVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEPEAA 130
                 EV+K +    E E E++V EE+  + ++  + E    E+V  V   E  E E  
Sbjct: 138 EE----EVEKVEVEVGEEEEEEEVKEEEVEEEVEVEEEE----EEVEEVEEIEVEEEEEK 197

Query: 131 AEAPNNPDPELAADADVPNNGALEVVAEDAPPNKDGADDAPADGAAPNKGADEVPA---- 190
            E       E   + +  +   +E   E+    ++  ++   +     +  +EV      
Sbjct: 198 VEEVEEEVVE--EEEEQEDEEEVEEEEEEEEEVEEEEEEEEVELEEEEEEVEEVVEIEVE 257

Query: 191 DEERAPKEGATELPEEGAAPNEGAGKLPAPNKEVDEVPEEEAAPNKEAEEFAD-EDEAPN 250
           +EE   +E   E+ EE         +     +EV+E   EE    +E EE  + E E   
Sbjct: 258 EEEEEVEEVEVEVEEEEEEMEVEVEEEEEEEEEVEEEVVEEEEEQEEEEEVEEVEVEVGE 317

Query: 251 PKELNPVEDEETPNKDEAEVAAEEGALEEDAPVEAEAREKEKGEEVEVEKEEEPKPKEEE 310
            +E   V++EE   ++E EV  EE  +EE    E    E+E+ EE EVE+EEE +  E+E
Sbjct: 318 EEEEEEVKEEEV--EEEVEVEEEEEEVEEVEEEEVVEEEEEQEEEEEVEEEEEEEEVEDE 377

Query: 311 -EGLDV---GLGNGEEEEVGFEEEEEEDERDKGEGDDPKEKLVDPKEKPV--------VE 359
            E ++V    +   EEEEV  EEEEEE+E  + E ++ ++ +V+ +E+ V        VE
Sbjct: 378 VEEIEVEEEEVEEEEEEEVEVEEEEEEEEVVEVEEEEEEDVVVEVEEEEVEVEEEVVEVE 431

HSP 4 Score: 91.7 bits (226), Expect = 2.0e-15
Identity = 93/334 (27.84%), Postives = 148/334 (44.31%), Query Frame = 1

Query: 31  EDTLVILVVLDKEPVGNENPEGEATNGEEEVKFWEELEERVLWNGEVDKDDANKFEVEAE 90
           +D   + +   K  V  E+ +GE     E+VK  EE EE      EV++++  + EVE E
Sbjct: 20  DDDKELYISCKKSKVAIESKQGEEVE-VEDVKVEEEEEEE---EEEVEEEEVEE-EVELE 79

Query: 91  DKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEPEAAAEAPNNPDPELAADADVPNNG 150
           ++  EE   + ++  + E  + E+          E E   E     + E   + +V    
Sbjct: 80  EEEVEEVEVEEIEVEEEEEEVEEE----------EEEMEVEVEVEVEEEEEEEVEVEEEV 139

Query: 151 ALEVVAEDAPPNKDGADDAPADGAAPNKGADEVPADEERAPKEGATELPEEGAAPNEGAG 210
             EVV E+    ++   +             EV   EE   +E   E  EE     E   
Sbjct: 140 EEEVVEEEEKQEEEEEVEKV-----------EVEVGEEEEEEEVKEEEVEEEVEVEE--- 199

Query: 211 KLPAPNKEVDEVPEEEAAPNKEAEEFADEDEAPNPKELNPVEDEETPNKDEAEVAAEEGA 270
                 +EV+EV E E    +E  E  +E+     +E    E+ E   ++E EV  EE  
Sbjct: 200 ----EEEEVEEVEEIEVEEEEEKVEEVEEEVVEEEEEQEDEEEVEEEEEEEEEVEEEEE- 259

Query: 271 LEEDAPVEAEAREKEKGEEVEVEKEEEP------KPKEEEEGLDVGLGNGEEEEVGFEEE 330
            EE+  +E E  E E+  E+EVE+EEE       + +EEEE ++V +   EEEE   EEE
Sbjct: 260 -EEEVELEEEEEEVEEVVEIEVEEEEEEVEEVEVEVEEEEEEMEVEVEEEEEEEEEVEEE 318

Query: 331 EEEDERDKGEGDDPKEKLVDPKEKPVVEENGEED 359
             E+E ++ E ++ +E  V+  E+   EE  EE+
Sbjct: 320 VVEEEEEQEEEEEVEEVEVEVGEEEEEEEVKEEE 318

HSP 5 Score: 67.0 bits (162), Expect = 5.3e-08
Identity = 53/158 (33.54%), Postives = 84/158 (53.16%), Query Frame = 1

Query: 204 APNEGAGKLPAPNKEVDEVPEEEAAPNKEAEEFADEDEAPNPKELNPVEDEETPNKDEAE 263
           A +EG+       K      ++E   + +  + A E +     E+  V+ EE   ++E E
Sbjct: 2   AAHEGSDTTTMSAKRSFVDDDKELYISCKKSKVAIESKQGEEVEVEDVKVEEEEEEEEEE 61

Query: 264 VAAEEGALEEDAPVEAEAREKEKGEEVEVEKEEEPKPKEEEE---GLDVGLGNGEEEEVG 323
           V  EE  +EE+  +E E  E+ + EE+EVE+EEE   +EEEE    ++V +   EEEEV 
Sbjct: 62  VEEEE--VEEEVELEEEEVEEVEVEEIEVEEEEEEVEEEEEEMEVEVEVEVEEEEEEEVE 121

Query: 324 FEEEEEEDERDKGEGDDPKEKLVDPKEKPVVEENGEED 359
            EEE EE+  ++ E  + +E+ V+  E  V EE  EE+
Sbjct: 122 VEEEVEEEVVEEEEKQEEEEE-VEKVEVEVGEEEEEEE 156

HSP 6 Score: 124.4 bits (311), Expect = 2.8e-25
Identity = 111/366 (30.33%), Postives = 173/366 (47.27%), Query Frame = 1

Query: 14  EKLEELVEASVREEEEE---------EDTLVILVVLDKEPVGNENPEGEATNGEEEVKFW 73
           E++EE+VE  V EEEEE         E+   + V +++E    E  E E    EEE +  
Sbjct: 265 EEVEEVVEIEVEEEEEEVEEVEVEVEEEEEEMEVEVEEEEEEEEEVEEEVVEEEEEQEEE 324

Query: 74  EELEERVLWNGEVDKDDANKFEVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETP 133
           EE+EE  +  GE ++++  K E E E++V  E+  + ++  + E V+ E+       E  
Sbjct: 325 EEVEEVEVEVGEEEEEEEVK-EEEVEEEVEVEEEEEEVEEVEEEEVVEEEEEQEEEEEVE 384

Query: 134 EPEAAAEAPNNPDPELAADADVPNNGALEVVAEDAPPNKD--GADDAPADGAAPNKGADE 193
           E E   E  +  +     + +V      EV  E+    ++    ++   +        +E
Sbjct: 385 EEEEEEEVEDEVEEIEVEEEEVEEEEEEEVEVEEEEEEEEVVEVEEEEEEDVVVEVEEEE 444

Query: 194 VPADEERAPKEGATELPEEGAAPNEGAGKLPAPNKEVDEVPEEEAAPNKEAEEFADEDEA 253
           V  +EE    E   E  EE     E   +     +E ++  EEE    +E EE  DE E 
Sbjct: 445 VEVEEEVVEVEEEEEEDEEEEEEVEEVEEEEVVEEEEEQEEEEEVEEEEEEEEVEDEVEE 504

Query: 254 PNPKELNPVEDEETPNKDEAEVAAEEGALEEDAPVEAEAREKEKGEE---VEVEKEEEPK 313
                    E+EE    +E EV  EE  +EE+   E E  E+E+ EE   VEVE+EEE +
Sbjct: 505 EE-------EEEEVEEVEEIEVEEEEEEVEEEEEEEVEVEEEEEEEEEEVVEVEEEEEEE 564

Query: 314 PKEEEEGLDVGLGNG----EEEEVGFEEEEEEDE-RDKG--EGDDPKEKLVDPKEKPVVE 359
            +EEEE + V +       EEE V  EEEEEE+E  ++G  EG+D +EK ++ K +  V+
Sbjct: 565 EEEEEEDVVVEVEEEEVEVEEEVVEVEEEEEEEEVEEEGEVEGEDEEEKEMEEKVEEKVK 622

BLAST of Cucsa.120710.1 vs. TrEMBL
Match: G7ZVJ6_MEDTR (Putative uncharacterized protein OS=Medicago truncatula GN=MTR_027s0011 PE=4 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 8.4e-22
Identity = 114/362 (31.49%), Postives = 166/362 (45.86%), Query Frame = 1

Query: 14  EKLEELVEASVREEEEEEDTLVILV-----VLDKEPVGNENPEGEATNGEEEVKFWEELE 73
           E+  E VE  V EEEEEE+     V     V ++E    E  E E    EE+V   EE+E
Sbjct: 167 EEEVEKVEVEVGEEEEEEEVKEEEVEEEVEVEEEEEEVEEVEEIEVEEEEEKV---EEVE 226

Query: 74  ERVLWNGEVDKDDAN-KFEVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEPE 133
           E V+   E  +D+   + E E E++V EE+  + ++  + E  + E V      E  E E
Sbjct: 227 EEVVEEEEEQEDEEEVEEEEEEEEEVEEEEEEEEVELEEEEEEVEEVVEIEVEEEEEEVE 286

Query: 134 AAAEAPNNPDPELAADADVPNNGALEVVAEDAPPNKDGADDAPADGAAPNKGADEVPADE 193
                    + E+  + +       EV  E     ++  ++   +      G +E   +E
Sbjct: 287 EVEVEVEEEEEEMEVEVEEEEEEEEEVEEEVVEEEEEQEEEEEVEEVEVEVGEEE---EE 346

Query: 194 ERAPKEGATELPEEGAAPNEGAGKLPAPNKEVDEVPEEEAAPNKEAEEFADEDEAPNPKE 253
           E   +E   E+ EE     E         +EV E  EEE    +E EE  +E+E  +  E
Sbjct: 347 EEVKEE---EVEEEVEVEEEEEEVEEVEEEEVVE-EEEEQEEEEEVEEEEEEEEVEDEVE 406

Query: 254 LNPVEDEETPNKDEAEVAAEEGALEE-----------DAPVEAEAREKEKGEE-VEVEKE 313
              VE+EE   ++E EV  EE   EE           D  VE E  E E  EE VEVE+E
Sbjct: 407 EIEVEEEEVEEEEEEEVEVEEEEEEEEVVEVEEEEEEDVVVEVEEEEVEVEEEVVEVEEE 466

Query: 314 EEPKPKEEEEGLDVGLGNGEEEEVGFEEEEEEDERDKGEGDDPKEKLVDPKEKPVVEENG 358
           EE   +EEEE  +V     EEEEV  EEEEE++E ++ E ++ +E++ D  E+   EE  
Sbjct: 467 EEEDEEEEEEVEEV-----EEEEV-VEEEEEQEEEEEVEEEEEEEEVEDEVEEEEEEEEV 512

HSP 2 Score: 108.2 bits (269), Expect = 2.1e-20
Identity = 106/345 (30.72%), Postives = 155/345 (44.93%), Query Frame = 1

Query: 14  EKLEELVEASVREEEEEEDTLVILVVLDKEPVGNENPEGEATNGEEEVKFWEELEERVLW 73
           E+ EE  E  V EEE EE+     V L++E V  E  E E    EEE +  EE EE +  
Sbjct: 81  EEEEEEEEEEVEEEEVEEE-----VELEEEEV--EEVEVEEIEVEEEEEEVEEEEEEMEV 140

Query: 74  NGEVDKDDANKFEVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEPEAAAEAP 133
             EV+ ++  + EVE E++V EE   +     + E V           E  E E   E  
Sbjct: 141 EVEVEVEEEEEEEVEVEEEVEEEVVEEEEKQEEEEEV-----------EKVEVEVGEEEE 200

Query: 134 NNPDPELAADADVPNNGALEVVAEDAPPNKDGADDAPADGAAPNKGADEVPADEERAPKE 193
                E   + +V      E V E                       +E+  +EE    E
Sbjct: 201 EEEVKEEEVEEEVEVEEEEEEVEE----------------------VEEIEVEEEEEKVE 260

Query: 194 GATELPEEGAAPNEGAGKLPAPNKEVDEVPEEEAAPNKEAEEFADEDEAPNPKELNPVED 253
              E   E     E   ++    +E +EV EEE     E EE  +E+E     E+   E+
Sbjct: 261 EVEEEVVEEEEEQEDEEEVEEEEEEEEEVEEEEEEEEVELEE--EEEEVEEVVEIEVEEE 320

Query: 254 EETPNKDEAEVAAEEGALEEDAPVEAEAREKEKGEEVEVEKEEEPKPKEEEEGLDVGLGN 313
           EE   + E EV  EE  +E +  VE E  E+E+ EE  VE+EEE + +EE E ++V +G 
Sbjct: 321 EEEVEEVEVEVEEEEEEMEVE--VEEEEEEEEEVEEEVVEEEEEQEEEEEVEEVEVEVGE 377

Query: 314 GEEEEVGFEEEEEEDERDKGEGDDPKEKLVDPKEKPVVEENGEED 359
            EEEE   EEE EE+     E ++ +E++ + +E+ VVEE  E++
Sbjct: 381 EEEEEEVKEEEVEEEV----EVEEEEEEVEEVEEEEVVEEEEEQE 377

HSP 3 Score: 102.1 bits (253), Expect = 1.5e-18
Identity = 107/366 (29.23%), Postives = 171/366 (46.72%), Query Frame = 1

Query: 11  VPNEKLEEL-VEASVREEEEEEDTLVILVVLDKEPVGNENPEGEATNGEEEVKFWEELEE 70
           V   ++EE+ VE    E EEEE+ + + V ++ E    E  E E    EE V+  E+ EE
Sbjct: 107 VEEVEVEEIEVEEEEEEVEEEEEEMEVEVEVEVEEEEEEEVEVEEEVEEEVVEEEEKQEE 166

Query: 71  RVLWNGEVDKDDANKFEVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEPEAA 130
                 EV+K +    E E E++V EE+  + ++  + E    E+V  V   E  E E  
Sbjct: 167 EE----EVEKVEVEVGEEEEEEEVKEEEVEEEVEVEEEE----EEVEEVEEIEVEEEEEK 226

Query: 131 AEAPNNPDPELAADADVPNNGALEVVAEDAPPNKDGADDAPADGAAPNKGADEVPA---- 190
            E       E   + +  +   +E   E+    ++  ++   +     +  +EV      
Sbjct: 227 VEEVEEEVVE--EEEEQEDEEEVEEEEEEEEEVEEEEEEEEVELEEEEEEVEEVVEIEVE 286

Query: 191 DEERAPKEGATELPEEGAAPNEGAGKLPAPNKEVDEVPEEEAAPNKEAEEFAD-EDEAPN 250
           +EE   +E   E+ EE         +     +EV+E   EE    +E EE  + E E   
Sbjct: 287 EEEEEVEEVEVEVEEEEEEMEVEVEEEEEEEEEVEEEVVEEEEEQEEEEEVEEVEVEVGE 346

Query: 251 PKELNPVEDEETPNKDEAEVAAEEGALEEDAPVEAEAREKEKGEEVEVEKEEEPKPKEEE 310
            +E   V++EE   ++E EV  EE  +EE    E    E+E+ EE EVE+EEE +  E+E
Sbjct: 347 EEEEEEVKEEEV--EEEVEVEEEEEEVEEVEEEEVVEEEEEQEEEEEVEEEEEEEEVEDE 406

Query: 311 -EGLDV---GLGNGEEEEVGFEEEEEEDERDKGEGDDPKEKLVDPKEKPV--------VE 359
            E ++V    +   EEEEV  EEEEEE+E  + E ++ ++ +V+ +E+ V        VE
Sbjct: 407 VEEIEVEEEEVEEEEEEEVEVEEEEEEEEVVEVEEEEEEDVVVEVEEEEVEVEEEVVEVE 460

HSP 4 Score: 91.7 bits (226), Expect = 2.0e-15
Identity = 93/334 (27.84%), Postives = 148/334 (44.31%), Query Frame = 1

Query: 31  EDTLVILVVLDKEPVGNENPEGEATNGEEEVKFWEELEERVLWNGEVDKDDANKFEVEAE 90
           +D   + +   K  V  E+ +GE     E+VK  EE EE      EV++++  + EVE E
Sbjct: 49  DDDKELYISCKKSKVAIESKQGEEVE-VEDVKVEEEEEEE---EEEVEEEEVEE-EVELE 108

Query: 91  DKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEPEAAAEAPNNPDPELAADADVPNNG 150
           ++  EE   + ++  + E  + E+          E E   E     + E   + +V    
Sbjct: 109 EEEVEEVEVEEIEVEEEEEEVEEE----------EEEMEVEVEVEVEEEEEEEVEVEEEV 168

Query: 151 ALEVVAEDAPPNKDGADDAPADGAAPNKGADEVPADEERAPKEGATELPEEGAAPNEGAG 210
             EVV E+    ++   +             EV   EE   +E   E  EE     E   
Sbjct: 169 EEEVVEEEEKQEEEEEVEKV-----------EVEVGEEEEEEEVKEEEVEEEVEVEE--- 228

Query: 211 KLPAPNKEVDEVPEEEAAPNKEAEEFADEDEAPNPKELNPVEDEETPNKDEAEVAAEEGA 270
                 +EV+EV E E    +E  E  +E+     +E    E+ E   ++E EV  EE  
Sbjct: 229 ----EEEEVEEVEEIEVEEEEEKVEEVEEEVVEEEEEQEDEEEVEEEEEEEEEVEEEEE- 288

Query: 271 LEEDAPVEAEAREKEKGEEVEVEKEEEP------KPKEEEEGLDVGLGNGEEEEVGFEEE 330
            EE+  +E E  E E+  E+EVE+EEE       + +EEEE ++V +   EEEE   EEE
Sbjct: 289 -EEEVELEEEEEEVEEVVEIEVEEEEEEVEEVEVEVEEEEEEMEVEVEEEEEEEEEVEEE 347

Query: 331 EEEDERDKGEGDDPKEKLVDPKEKPVVEENGEED 359
             E+E ++ E ++ +E  V+  E+   EE  EE+
Sbjct: 349 VVEEEEEQEEEEEVEEVEVEVGEEEEEEEVKEEE 347

HSP 5 Score: 69.7 bits (169), Expect = 8.2e-09
Identity = 60/178 (33.71%), Postives = 93/178 (52.25%), Query Frame = 1

Query: 187 EERAPK-EGATELPEEGA--APNEGAGKLPAPNKEVDEVPEEEAAPNKEAEEFADEDEAP 246
           +E  PK EG  E   E +  A +EG+       K      ++E   + +  + A E +  
Sbjct: 11  KENTPKGEGGREGYRENSIMAAHEGSDTTTMSAKRSFVDDDKELYISCKKSKVAIESKQG 70

Query: 247 NPKELNPVEDEETPNKDEAEVAAEEGALEEDAPVEAEAREKEKGEEVEVEKEEEPKPKEE 306
              E+  V+ EE   ++E EV  EE  +EE+  +E E  E+ + EE+EVE+EEE   +EE
Sbjct: 71  EEVEVEDVKVEEEEEEEEEEVEEEE--VEEEVELEEEEVEEVEVEEIEVEEEEEEVEEEE 130

Query: 307 EE---GLDVGLGNGEEEEVGFEEEEEEDERDKGEGDDPKEKLVDPKEKPVVEENGEED 359
           EE    ++V +   EEEEV  EEE EE+  ++ E  + +E+ V+  E  V EE  EE+
Sbjct: 131 EEMEVEVEVEVEEEEEEEVEVEEEVEEEVVEEEEKQEEEEE-VEKVEVEVGEEEEEEE 185

HSP 6 Score: 122.1 bits (305), Expect = 1.4e-24
Identity = 103/345 (29.86%), Postives = 153/345 (44.35%), Query Frame = 1

Query: 14  EKLEELVEASVREEEEEEDTLVILVVLDKEPVGNENPEGEATNGEEEVKFWEELEERVLW 73
           E+ EE VE    EEEEEE+        ++E    E  E E    EEE +  EE EE V  
Sbjct: 26  EEEEEEVEEEEEEEEEEEE--------EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEVEE 85

Query: 74  NGEVDKDDANKFEVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEPEAAAEAP 133
            GE ++++  + E E E++  EE+  +  +  + E    E+       E  E E   E  
Sbjct: 86  EGEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEGEEEEEEEEEEEEE 145

Query: 134 NNPDPELAADADVPNNGALEVVAEDAPPNKDGADDAPADGAAPNKGADEVPADEERAPKE 193
              + E   + +    G  E   E+    ++             +G +E   +EE   +E
Sbjct: 146 EEEEEEEEEEEEEEEEGEEEEEEEEEEEEEE-----------EEEGEEEEEEEEEEEEEE 205

Query: 194 GATELPEEGAAPNEGAGKLPAPNKEVDEVPEEEAAPNKEAEEFADEDEAPNPKELNPVED 253
              E  EE     E          E +E  EEE    +E EE  +E+E    KE    E 
Sbjct: 206 EEEEEEEEEEEEEE----------EEEEEEEEEEEEGEEEEEEEEEEEEEEEKE----EG 265

Query: 254 EETPNKDEAEVAAEEGALEEDAPVEAEAREKEKGEEVEVEKEEEPKPKEEEEGLDVGLGN 313
           EE   ++E E   EE   EE+   E E  E+E+ EE E E+EEE + +EEEE  +     
Sbjct: 266 EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE 325

Query: 314 GEEEEVGFEEEEEEDERDKGEGDDPKEKLVDPKEKPVVEENGEED 359
            EEEE   EEEEEE+E ++ E ++ +E+  + +E    EE  EE+
Sbjct: 326 EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEE 337

BLAST of Cucsa.120710.1 vs. TrEMBL
Match: A0A0L8FLA1_OCTBM (Uncharacterized protein (Fragment) OS=Octopus bimaculoides GN=OCBIM_22015442mg PE=4 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 2.4e-16
Identity = 65/182 (35.71%), Postives = 95/182 (52.20%), Query Frame = 1

Query: 186 DEERAPKEGATELPEEGAAPNEGAGKLPAPNKEVDEVPEEEAAPNKEAEEFADEDEAPNP 245
           +EE   +E   E+ EE     E         +E +EV EEE    +E EE  +E+E    
Sbjct: 3   EEEEEEEEDEEEVEEEVEEEEE-------EEEEEEEVEEEEEEEEEEEEEEEEEEEEEEE 62

Query: 246 KELNPVEDEETPNKDEAEVAAEEGALEEDAPVEAEAREKEKGEEVEVEKEEEPKPKEEEE 305
           +E    E+EE   ++E E   EEG  EE+   E E  E+E+ EE E E+EEE + +EEEE
Sbjct: 63  EEEEEEEEEEEEEEEEEEEVEEEGEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE 122

Query: 306 GLDVGLGNGEEEEVGFEEEE---------EEDERDKGEGDDPKEKLVDPKEKPVVEENGE 359
             +     GEEEE   EEEE         EE+E ++ EG++ +E+  + +E+   EE GE
Sbjct: 123 EEEEEEEEGEEEEEEEEEEEEEEEEEEEEEEEEEEEEEGEEEEEE--EEEEEEEEEEEGE 175

HSP 2 Score: 122.1 bits (305), Expect = 1.4e-24
Identity = 117/368 (31.79%), Postives = 165/368 (44.84%), Query Frame = 1

Query: 13  NEKLEELVEASVREEEEEEDTLVILVVLDKEPVGNENPEGEATNGEEEVKFWEELEERVL 72
           NE+ EE  E    EEEEEE+        ++E    E  E E    EEE    EE+EE   
Sbjct: 243 NEEGEEEEEEEEEEEEEEEEEEE-----EEEGEEEEEEEEEEEEEEEEE---EEVEEG-- 302

Query: 73  WNGEVDKDDANKFE-------VEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPE 132
            NG+  K++  + E        E  D+  E    KG D +K E    E+       E  E
Sbjct: 303 GNGKEGKEEEEEEEGEEGANKQEEGDEGGEGGGGKGKDNDKEEEEEEEE-----EEEEEE 362

Query: 133 PEAAAEAPNNPDPELAADADV--PNNGALEVVAEDAPPNKDGADDAPADGAAPNKGADEV 192
              A E     D E   + +      G  E +AE+       A  A A   A ++   EV
Sbjct: 363 EGGAEEEEEEEDEEEEGEEEEGEEGEGGEEGIAEEEEEEAAAAATATATATASSEEEGEV 422

Query: 193 PADEERAPKEGAT----ELPEEGAAPNEGAGKLPAPNKEVDEVPEEEAAPNKEAEEFADE 252
             DE  A    A     +  EEG A  E  G+     +E +E  EEE    +E EE    
Sbjct: 423 DDDEGAAAAAAAAAAGEDEEEEGIAEEEEEGE----EEEEEEGDEEEEEEEEEEEEGEGG 482

Query: 253 DEAPNPKELNPVEDEETPNKDEAEVAAEEGALEEDAPVEAEAREKEKGEEVEVEKEEEPK 312
           +E    +E    E+E    ++E E+    G  E+D   E E  E+E+ EE E E+EEE +
Sbjct: 483 EEGRRREEEEEEEEEGEEEEEEEEIGGGGGGGEDDDEEEEEEEEEEEEEEEEEEEEEEEE 542

Query: 313 PKEEEEGLDVGLG----NGEEEEVGFEEEEEEDER-----DKGEGDDPKEKLVDPKEKPV 359
            +EEEE  ++G+G      EEEE G EEEEEE+E      + GEG++ +E+  + +EK +
Sbjct: 543 EEEEEEEGELGVGVSAVEEEEEEEGKEEEEEEEEEKEEEGEGGEGEEEEEEEEEEEEKRI 591

BLAST of Cucsa.120710.1 vs. TAIR10
Match: AT4G37820.1 (AT4G37820.1 unknown protein)

HSP 1 Score: 83.2 bits (204), Expect = 3.6e-16
Identity = 89/353 (25.21%), Postives = 138/353 (39.09%), Query Frame = 1

Query: 10  SVPNEKLEELVEASVREEEEEEDTLVILVVLDKEPVGNENPEG--EATNGEEEVKFWEEL 69
           S   E  +E+VE    E+  EE+        +KE  G  N EG  ++ NGE E K  +E 
Sbjct: 98  STDKENGDEIVEREEEEKAVEENN-------EKEAEGTGNEEGNEDSNNGESE-KVVDES 157

Query: 70  EERVLWNGEVDKDDANKFEVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEPE 129
           E       E+  ++A +   + +D   E     G +    E V   +V G     + E  
Sbjct: 158 EG----GNEISNEEAREINYKGDDASSE--VMHGTEEKSNEKV---EVEGESKSNSTENV 217

Query: 130 AAAEAPNNPDPELAADADVPNNGALEVVAEDAPPNKDGADDAPADGAAPNKGADEVPAD- 189
           +  E  + P  E+  +  V    +L      +   +     +  D     KG  +   + 
Sbjct: 218 SVHEDESGPKNEVL-EGSVIKEVSLNTTENGSDDGEQQETKSELDSKTGEKGFSDSNGEL 277

Query: 190 -EERAPKEGATELPEEGAAPNEGA-GKLPAPNKEVDEVPEEEAAPNKEAEEFADEDEAPN 249
            E       ATE  E   +   G+ GK     +  +E  E+E   + E EE   ++   N
Sbjct: 278 PETNLSTSNATETTESSGSDESGSSGKSTGYQQTKNEEDEKEKVQSSE-EESKVKESGKN 337

Query: 250 PKELNPVEDEETPNKDEAEVAAEEGALEEDAPVEAEAREKEKGEEVEVEKEEEPKPKEEE 309
            K+ +  +DE    K E +   E  +  E    E E REKE     E  KEEEP+ KE+E
Sbjct: 338 EKDASSSQDESKEEKPERKKKEESSSQGEGKEEEPEKREKEDSSSQEESKEEEPENKEKE 397

Query: 310 EGLDVGLGNGEEEEVGFEEEEEEDERDKGEGDDPKEKLVDPKEKPVVEENGEE 358
                   + EE E+   E +E++E    EG++ KE      E    E    E
Sbjct: 398 AS-----SSQEENEIKETEIKEKEESSSQEGNENKETEKKSSESQRKENTNSE 426

HSP 2 Score: 43.5 bits (101), Expect = 3.2e-04
Identity = 37/102 (36.27%), Postives = 50/102 (49.02%), Query Frame = 1

Query: 246 KELNPVEDEETPNKDEAEVAAEEGALEEDAPVEAEAREKEKGEEVEVEKEEEPKPKEEEE 305
           K+L P  +E    KDE E   EEG+  E       + +KE G+E+ VE+EEE K  EE  
Sbjct: 67  KDLRPRIEETKDVKDEVE--DEEGSKNEGGG--DVSTDKENGDEI-VEREEEEKAVEE-- 126

Query: 306 GLDVGLGNGEEEEVGFEEEEEEDERDKGEGDDPKEKLVDPKE 348
                  N E+E  G   EE  ++ + GE     EK+VD  E
Sbjct: 127 -------NNEKEAEGTGNEEGNEDSNNGE----SEKVVDESE 150

HSP 3 Score: 41.6 bits (96), Expect = 1.2e-03
Identity = 40/136 (29.41%), Postives = 61/136 (44.85%), Query Frame = 1

Query: 220 DEVPEEEAAPNKEAEEFADEDEAPNPKELNPVEDEETPNKDEAEVAAEEGALEEDAPVEA 279
           DEV +EE + N+   + + + E  N  E+  VE EE           EE A+EE+   EA
Sbjct: 81  DEVEDEEGSKNEGGGDVSTDKE--NGDEI--VEREE-----------EEKAVEENNEKEA 140

Query: 280 EAREKEKGEEVEVEKEEEPKPKEEEEGLDVGLGNGEEEEVGFEEEEEEDERDKGEGDDPK 339
           E    E+G E     E E    E E G ++   N E  E+ ++ ++   E   G  +   
Sbjct: 141 EGTGNEEGNEDSNNGESEKVVDESEGGNEI--SNEEAREINYKGDDASSEVMHGTEEKSN 199

Query: 340 EKL-VDPKEKPVVEEN 355
           EK+ V+ + K    EN
Sbjct: 201 EKVEVEGESKSNSTEN 199

BLAST of Cucsa.120710.1 vs. TAIR10
Match: AT5G55660.1 (AT5G55660.1 DEK domain-containing chromatin associated protein)

HSP 1 Score: 74.7 bits (182), Expect = 1.3e-13
Identity = 92/343 (26.82%), Postives = 147/343 (42.86%), Query Frame = 1

Query: 30  EEDTLVILVVLDKEPVGNENPE----GEATNGEE--EVKFWEELEERVLWNGEVDKDDAN 89
           EEDT VI+     EP  N         +A +G+E  E    +E++E    + + +     
Sbjct: 3   EEDTKVIV-----EPTANGTSSLQKTSDAISGKEVQENASGKEVQE----SKKEEDTGLE 62

Query: 90  KFEVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEPEAAAEAPNNPDPELAAD 149
           K E++ E K  E ++  G   +K   V  E+   V   +  +PEA     +  D  L AD
Sbjct: 63  KMEIDDEGKQHEGESETG---DKEVEVTEEEKKDV-GEDKEQPEADKMDEDTDDKNLKAD 122

Query: 150 ADVPNNGALEVVAEDAPPNKDGADDAPADGAAPNKGADEVPADEERAP-------KEGAT 209
                +G   V  E+    K+  + A    A   +G  E  + EE+         +EG T
Sbjct: 123 -----DGVSGVATEEDAVMKESVESADNKDAENPEGEQEKESKEEKLEGGKANGNEEGDT 182

Query: 210 ELPEEGAAPNEGAGKLPAPNKEVDEVPEEEAAPNKEAEEFADEDEAPNPKELNPVEDEET 269
           E    G    +   +     + VDE  +EEA   K   E A+E+E    +E+     E+ 
Sbjct: 183 EEKLVGGDKGDDVDEAEKV-ENVDEDDKEEALKEKNEAELAEEEETNKGEEVKEANKEDD 242

Query: 270 PNKDEAEVAAEEGALEEDAPVEAEAREKEKGEEVEVEKEE--EPKPKEEEEGLDVGLGNG 329
              D  +VA  E    ED   E++   ++K EE E EKEE  + K  E+EE  D    + 
Sbjct: 243 VEAD-TKVAEPE---VEDKKTESKDENEDKEEEKEDEKEESMDDKEDEKEESNDDDKEDE 302

Query: 330 EEEEVGFEEEEEED-----ERDKGEGDDPKEKLVDPKEKPVVE 353
           +EE    +E+++ED     +R KG+ +  + K    +EK  +E
Sbjct: 303 KEESNDDKEDKKEDIKKSNKRGKGKTEKTRGKTKSDEEKKDIE 322

HSP 2 Score: 38.9 bits (89), Expect = 7.8e-03
Identity = 37/128 (28.91%), Postives = 56/128 (43.75%), Query Frame = 1

Query: 237 ADEDEAPNPKELNPVEDEETPNKDEAEV---AAEEGALEEDAPVEAEAREKEKGEEVEVE 296
           A  D   N KE   V+ + TP K        +++  A  +    EA    K+     + E
Sbjct: 470 ATTDVLVNEKEKG-VKRKRTPKKSSPAAGSSSSKRSAKSQKKTEEATRTNKKSVAHSDDE 529

Query: 297 KEEEPKPKEEEEGLDVGLGNGEEEEVGFEEEEEEDERDKG---EGDDPKEKLVDPKEKPV 356
            EEE +  EEEE         +E+EV    EEEE+E + G   + +D   +L + +E   
Sbjct: 530 SEEEKEDDEEEE---------KEQEV----EEEEEENENGIPDKSEDEAPQLSESEENVE 583

Query: 357 VEENGEED 359
            EE  EE+
Sbjct: 590 SEEESEEE 583

HSP 3 Score: 38.5 bits (88), Expect = 1.0e-02
Identity = 35/138 (25.36%), Postives = 56/138 (40.58%), Query Frame = 1

Query: 218 EVDEVPEEEAAPNKEAEEFADEDEAPNPKELNPVEDEETPNKDEAEVAAEEGALEEDAPV 277
           E ++  + +  P K +          + K     E+    NK     + +E   E++   
Sbjct: 478 EKEKGVKRKRTPKKSSPAAGSSSSKRSAKSQKKTEEATRTNKKSVAHSDDESEEEKE--- 537

Query: 278 EAEAREKEKGEEVEVEKEEEPK---PKEEEEGLDVGLGNGEEEEVGFEEEEEEDERDKGE 337
             +  E+EK +EVE E+EE       K E+E   +   +  EE V  EEE EE+ + K  
Sbjct: 538 --DDEEEEKEQEVEEEEEENENGIPDKSEDEAPQL---SESEENVESEEESEEETKKKKR 597

Query: 338 G----DDPKEKLVDPKEK 349
           G     D KE     + K
Sbjct: 598 GSRTSSDKKESAGKSRSK 607

BLAST of Cucsa.120710.1 vs. TAIR10
Match: AT5G24880.1 (AT5G24880.1 BEST Arabidopsis thaliana protein match is: calmodulin-binding protein-related (TAIR:AT5G10660.1))

HSP 1 Score: 62.0 bits (149), Expect = 8.6e-10
Identity = 65/271 (23.99%), Postives = 108/271 (39.85%), Query Frame = 1

Query: 97  DAPKGLDPNKPEPVLPE----DVAGVPNRETPEPEAAAEAPNNPDPELAADADVPNNGAL 156
           + PKG +  K  PV P+    + +   ++   E           D E+A D+   ++   
Sbjct: 140 NVPKGGNTAKSPPVAPKKSGLNSSSTSSKSKKEGSENVRIKKASDKEIALDSASMSSAQE 199

Query: 157 EVVAEDAPPNKDGADDAPADGAAPNKGADEVPADEERAPKEGATELPEEGAAPNEGAGKL 216
           +   E      D    +  D   P    +E    E+      + E   E + P   A  +
Sbjct: 200 DHQEEILKVESDHLQVSDHDIEEPKYEKEEKEVQEKVVQANESVEEKAESSGPTPVASPV 259

Query: 217 PAPNKEVDEVPEEEAAPNKEAEEFADEDEAPNPKELNPVEDEETPNKDEAEVAAEEGALE 276
                 V    EE+   N        ED+     E    +D    NK E E   ++   E
Sbjct: 260 GKDCNAVVAELEEKLIKN--------EDDIEEKTEEMKEQDNNQANKSEEEEDVKKKIDE 319

Query: 277 EDAP--VEAEAREKEKGEEVEVEKEEEPKPKEEEEGLDVGLGNGEEEEVGFEEEEEEDER 336
            + P  V+ E++E E  EE   EKEEE K + +E   +      + +E   +E+ EE+E+
Sbjct: 320 NETPEKVDTESKEVESVEETTQEKEEEVKEEGKERVEEEEKEKEKVKEDDQKEKVEEEEK 379

Query: 337 DKGEGDDPKEKL-----VDPKEKPVVEENGE 357
           +K +GD+ KEK+      + K+K VV+   E
Sbjct: 380 EKVKGDEEKEKVKEEESAEGKKKEVVKGKKE 402

BLAST of Cucsa.120710.1 vs. TAIR10
Match: AT1G15940.1 (AT1G15940.1 Tudor/PWWP/MBT superfamily protein)

HSP 1 Score: 61.2 bits (147), Expect = 1.5e-09
Identity = 72/275 (26.18%), Postives = 106/275 (38.55%), Query Frame = 1

Query: 76  EVDKDDANKFEVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVP---NRETPEPEAAAEA 135
           +++KD   + ++E +D   EED+ K    +  EP   ED   +P   N ET       EA
Sbjct: 742 KIEKDCDEQEDLETKD---EEDSLKLGKESDAEPDRMEDHQELPENHNVETKTDGEEQEA 801

Query: 136 PNNPDPELAADADVPNNGALEVVAEDAPPNKDGADDAPADGAAPNKGADEVPADEERAPK 195
              P  E   + + PN          A P  DG +            +D    +E+ A K
Sbjct: 802 AKEPTAESKTNGEEPN----------AEPETDGKEHKSLKEPNAEPKSD---GEEQEAAK 861

Query: 196 EGATELPEEGAAPNEGAGKLPAPNKEVDEVPEEEAAPNKEAEEFADEDEAPNPKELNPVE 255
           E   EL  +G                      +EAA    AE   DE+E     E+    
Sbjct: 862 EPNAELKTDGE--------------------NQEAAKELTAERKTDEEEHKVADEVEQKS 921

Query: 256 DEETPNKDEAEVAAEEGALEEDAPVEAEAREKEKGEEVE-----VEKEEEPKPKEEEEGL 315
            +ET  + EAE   ++   E +A  + +  EKE  +E       +EKE+  K K EE   
Sbjct: 922 QKETNVEPEAEGEEQKSVEEPNAEPKTKVEEKESAKEQTADTKLIEKEDMSKTKGEEIDK 980

Query: 316 DVGLGNGEEEEVGFEEEEE--------EDERDKGE 335
           +      E  +VG E EE+        E+E DK E
Sbjct: 982 ETYSSIPETGKVGNEAEEDDQRVIKELEEESDKAE 980

BLAST of Cucsa.120710.1 vs. TAIR10
Match: AT5G64910.1 (AT5G64910.1 unknown protein)

HSP 1 Score: 59.7 bits (143), Expect = 4.3e-09
Identity = 44/148 (29.73%), Postives = 71/148 (47.97%), Query Frame = 1

Query: 208 GAGKLPAPNKE--VDEVPEEEAAPNKEAEEFADEDEAPNPKELNPVEDEETPNKDE--AE 267
           GA    + N +  V+    +E  P +E EE  D+ E+P P+E    E+E   N++E  A+
Sbjct: 22  GASSSASKNDDAVVEATTTQETQPTQETEETEDKVESPAPEEEGKNEEEANENQEEEAAK 81

Query: 268 V---AAEEGALEEDAPVEAEAREKEKGEEVEVEKEEEPKPKEEEEGLDVGLGNGEEEEVG 327
           V   AAEEG  EE+A  + E  ++E   E + E+EE  KP E     +   G        
Sbjct: 82  VESKAAEEGGNEEEAKEDKEEEKEEAAREDKEEEEEAVKPDESASQKEEAKGASSS---- 141

Query: 328 FEEEEEEDERDKGEGDDPKEKLVDPKEK 349
            E +    +R +G   + ++K+  P+ K
Sbjct: 142 -EPQLRRGKRKRGTKTEAEKKVSTPRAK 164

BLAST of Cucsa.120710.1 vs. NCBI nr
Match: gi|778722074|ref|XP_011658400.1| (PREDICTED: major centromere autoantigen B [Cucumis sativus])

HSP 1 Score: 176.0 bits (445), Expect = 1.2e-40
Identity = 95/120 (79.17%), Postives = 102/120 (85.00%), Query Frame = 1

Query: 236 FADEDEAPNPKELNPVEDEETPNKDEAEVAAEEGALEEDAPVEAEAREKEKG---EEVEV 295
           FADE+EA N KELNPVEDEET NKDEA VA EEG LE+DA VEAEAREKE G   EEVE 
Sbjct: 15  FADENEALNAKELNPVEDEETLNKDEAGVAEEEGVLEKDALVEAEAREKENGEKVEEVEE 74

Query: 296 EKEEEPKPKEEEEGLDVGLGNGEEEEVGF---EEEEEEDERDKGEGDDPKEKLVDPKEKP 350
           EKE+EPKPK EEEGL+VGLGNGE+EEVGF   EEEEEEDERDKG+GDDPKEK+ DPK KP
Sbjct: 75  EKEKEPKPK-EEEGLEVGLGNGEDEEVGFEEEEEEEEEDERDKGKGDDPKEKVEDPKPKP 133

BLAST of Cucsa.120710.1 vs. NCBI nr
Match: gi|778722074|ref|XP_011658400.1| (PREDICTED: major centromere autoantigen B [Cucumis sativus])

HSP 1 Score: 38.5 bits (88), Expect = 2.9e+01
Identity = 34/99 (34.34%), Postives = 50/99 (50.51%), Query Frame = 1

Query: 216 NKEVDEVPEEEAAPNKEAEEFADEDEAPNPKELNPVEDEET----PNKDEA-EVAA---- 275
           NK+   V EEE    K+A   A+  E  N +++  VE+E+     P ++E  EV      
Sbjct: 37  NKDEAGVAEEEGVLEKDALVEAEAREKENGEKVEEVEEEKEKEPKPKEEEGLEVGLGNGE 96

Query: 276 -EEGALEEDAPVEAEARE-KEKGEEVEVEKEEEPKPKEE 304
            EE   EE+   E E    K KG++ + EK E+PKPK +
Sbjct: 97  DEEVGFEEEEEEEEEDERDKGKGDDPK-EKVEDPKPKPD 134

HSP 2 Score: 129.8 bits (325), Expect = 9.6e-27
Identity = 124/379 (32.72%), Postives = 167/379 (44.06%), Query Frame = 1

Query: 7   PSRSVPNEKLEELVEASVREEEEEEDTLVILVVLDKEPVGNENPEGEATNGEEEVKFWEE 66
           P+   P E+     EA+  E  EEE         ++ P   E P  EA   EEE    E 
Sbjct: 145 PAEEAPAEEEAPAEEAAEEEHAEEE------APAEEAPAEEEAPAEEAV--EEEHAEEEA 204

Query: 67  LEERVLWNGEVDKDDANKFEVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEP 126
             E      E   ++A + E  AE++ P E+AP   +    E V  E        E P  
Sbjct: 205 PAEEAPAEEEAPAEEAAE-EEHAEEEAPAEEAPAEEEAPVEEAVEEEHAEEEEAEEAPAE 264

Query: 127 EAAAEAPNN----PDPELAADADVPNNGALEVV-----AEDAPPNKDGADDAPADGAAPN 186
           EA AE  +     P  E  A+ + P   A+E       AE+AP  +  A++APA+ A P 
Sbjct: 265 EAPAEEEHAEEEAPAEEAPAEEEAPEEAAVEEEHAEEEAEEAPVEEAAAEEAPAEEADPA 324

Query: 187 KGADEVPADEERAPKEGATELPEEGAAPNEGAGKLPAPNKE--------VDEVPEEEAAP 246
           + A E P    R  +    +  +E ++ +E A    AP KE        V+E  EEE+AP
Sbjct: 325 EAAHEEPPPLRRRKRSDDADEVKEESSEDEAAEVESAPEKEVATKEEEAVEEAVEEESAP 384

Query: 247 NKEAEEFADEDEA---PNPKELNPVEDEETPNKDEAEVAAEEGALEEDAPVEAEAREKEK 306
              AEE A E+EA      +E  P E+E    ++EA  A EE A EE AP E EA E+E 
Sbjct: 385 ---AEEHAAEEEAVEEATEEEAAPAEEEAA--EEEAAPAEEEAAEEEAAPAEEEAAEEEA 444

Query: 307 GEE----VEVEKEEEPKPKEEEEGLDVGLGNGE---EEEVGFEEEEEEDERDKGEGDDPK 359
            EE     E   EEE  P EEE   +      E   EEE   EEE  E+E    E   P+
Sbjct: 445 TEEEAHAEEEAAEEEAAPAEEEAAEEEAAPAEEEAAEEEAPAEEEAAEEEAHAEEEAAPE 504

BLAST of Cucsa.120710.1 vs. NCBI nr
Match: gi|919044932|ref|XP_013405651.1| (PREDICTED: fibrous sheath CABYR-binding protein-like isoform X2 [Lingula anatina])

HSP 1 Score: 116.7 bits (291), Expect = 8.4e-23
Identity = 121/365 (33.15%), Postives = 167/365 (45.75%), Query Frame = 1

Query: 7   PSRSVPNEK---LEELVEASVREEEEEEDTLVILVVLDKEPVGNENPEGEATNGEEEVKF 66
           P+   P E+   +EE VE    EEEE E+        ++E    E P  EA   EEE   
Sbjct: 223 PAEEAPAEEEAPVEEAVEEEHAEEEEAEEAPAEEAPAEEEHAEEEAPAEEAP-AEEEAPE 282

Query: 67  WEELEERVLWNGEVDKDDANKFEVEAEDKVPEEDAPKGLDPNKPEPVLPE---DVAGVPN 126
              +EE    + E + ++A   E  AE+   EE  P      +P P+      D A    
Sbjct: 283 EAAVEEE---HAEEEAEEAPVEEAAAEEAPAEEADPAEAAHEEPPPLRRRKRSDDADEVK 342

Query: 127 RETPEPEAAAEAPNNPDPELAADADVPNNGALE---VVAEDAPPNKDGADDAPADGAAPN 186
            E+ E EAA E  + P+ E+A   +     A+E     AE+    ++  ++A  + AAP 
Sbjct: 343 EESSEDEAA-EVESAPEKEVATKEEEAVEEAVEEESAPAEEHAAEEEAVEEATEEEAAP- 402

Query: 187 KGADEVPADEERAPKEGATELPEEGAAP-NEGAGKLPAPNKEV---DEVPEEEAAPNKEA 246
             A+E  A+EE AP E   E  EE AAP  E A +  A  +E    +E  EEEAAP +E 
Sbjct: 403 --AEEEAAEEEAAPAE--EEAAEEEAAPAEEEAAEEEATEEEAHAEEEAAEEEAAPAEE- 462

Query: 247 EEFADEDEAPNPKELNPVEDEETPNKDEAEVAAEEGALEEDAPVEAEAREKEKGEEVEVE 306
            E A+E+ AP  +E     +EE P ++EA    E  A EE AP E EA E+E G   E  
Sbjct: 463 -EAAEEEAAPAEEE---AAEEEAPAEEEA-AEEEAHAEEEAAP-EEEAAEEEAGPAEEEA 522

Query: 307 KEEEPKPKEEEEGLDVGLGNGEEEEVGFEEEEEEDERDKGEGDDPKEKLVDPKEKPVVEE 359
            EEE   +E  E          EEE   EEE  E+E    E +  +E+    +E    EE
Sbjct: 523 AEEEAPAEEAAEEEAPAEEEATEEEAPAEEEAAEEEEAPAEEEAAEEEAPAEEEAAHAEE 570

HSP 2 Score: 114.4 bits (285), Expect = 4.2e-22
Identity = 105/357 (29.41%), Postives = 152/357 (42.58%), Query Frame = 1

Query: 7   PSRSVPNEKLEELVEASVREEEEEEDTLVILVVLDKEPVGNENPEGEATNGEEEVKFWEE 66
           P+   P E+     EA+  E  EEE         ++ P   E+ E EA    EE    EE
Sbjct: 97  PAEEAPAEEEAPAEEAAEEEHAEEEAPAEEAPAEEEAPAEEEHAEEEAP--AEEAPAEEE 156

Query: 67  LEERVLWNGEVDKDDANKFEVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEP 126
                    E  +++A   E  AE++ P E+A   ++    E   P + A  P  E    
Sbjct: 157 APAEEAAEEEHAEEEAPAEEAPAEEEAPAEEA---VEEEHAEEEAPAEEA--PAEEEAPA 216

Query: 127 EAAAEAPNNPDPELAADADVPNNGALEVVAEDAPPNKDGADDAPADGAAPNKGADEVPAD 186
           E AAE  +  +   A +A       +E   E+    ++ A++AP         A+E PA+
Sbjct: 217 EEAAEEEHAEEEAPAEEAPAEEEAPVEEAVEEEHAEEEEAEEAP---------AEEAPAE 276

Query: 187 EERAPKEG-ATELPEEGAAPNEGAGKLPAPNKEVDEVPEEEAAPNKEAEEFADEDEAPNP 246
           EE A +E  A E P E  AP E A +     +E +E P EEAA  +   E AD  EA + 
Sbjct: 277 EEHAEEEAPAEEAPAEEEAPEEAAVEEEHAEEEAEEAPVEEAAAEEAPAEEADPAEAAHE 336

Query: 247 KELNPVEDEETPNKDEAEVAAEEGALEEDAPVEAEAREKE----KGEEVEVEKEEEPKPK 306
           +   P         D+A+   EE + +E A VE+ A EKE    + E VE   EEE  P 
Sbjct: 337 E---PPPLRRRKRSDDADEVKEESSEDEAAEVES-APEKEVATKEEEAVEEAVEEESAPA 396

Query: 307 EEEEGLDVGLGNGEEEEVGFEEEEEEDERDKGEGDDPKEKLVDPKEKPVVEENGEED 359
           EE    +  +    EEE    EEE  +E      ++  E+   P E+   EE   E+
Sbjct: 397 EEHAAEEEAVEEATEEEAAPAEEEAAEEEAAPAEEEAAEEEAAPAEEEAAEEEATEE 433

HSP 3 Score: 113.2 bits (282), Expect = 9.3e-22
Identity = 110/354 (31.07%), Postives = 152/354 (42.94%), Query Frame = 1

Query: 26  EEEEEEDTLVILVVLDKEPVGNENPEGEATNGEEEVKFWEELEERVLWNGEVDKDDANKF 85
           EE  +E+   +    ++ P  +E P  EA  G+EE    EE         E  +++A   
Sbjct: 42  EEAVKEEAAPVEQAAEEAPEEDEVPAEEA--GKEEAPAEEEAPAEEAVEEEHAEEEAPAE 101

Query: 86  EVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEPEAAAEAPNNPDPELAADAD 145
           E  AE++ P E+A +  + +  E    E+          E  A  EAP    P   A+ +
Sbjct: 102 EAPAEEEAPAEEAAE--EEHAEEEAPAEEAPAEEEAPAEEEHAEEEAPAEEAP---AEEE 161

Query: 146 VPNNGALEVVAEDAPPNKDGADDAPADGAAPNKGADEVPADEERAPKEGATELPEEGAAP 205
            P   A E   E+    +  A++APA+  AP + A E    EE AP E   E P E  AP
Sbjct: 162 AP---AEEAAEEEHAEEEAPAEEAPAEEEAPAEEAVEEEHAEEEAPAE---EAPAEEEAP 221

Query: 206 NEGAGKLPAPNKEV--DEVPEEEAAPNKEA--EEFADEDEAPN-PKELNPVEDEETPNKD 265
            E A +     +E   +E P EE AP +EA  EE A+E+EA   P E  P E+E      
Sbjct: 222 AEEAAEEEHAEEEAPAEEAPAEEEAPVEEAVEEEHAEEEEAEEAPAEEAPAEEEH----- 281

Query: 266 EAEVAAEEGALEEDAPVEAEAREKEKGEEVEVEKEEEPKPKEEEEGLDVGLGNGEEEEVG 325
                AEE A  E+AP E EA E+   EE   E+E E  P EE    +      +  E  
Sbjct: 282 -----AEEEAPAEEAPAEEEAPEEAAVEEEHAEEEAEEAPVEEAAAEEAPAEEADPAEAA 341

Query: 326 FEE----------------EEEEDERDKGEGDDPKEKLVDPKEKPVVEENGEED 359
            EE                +EE  E +  E +   EK V  KE+  VEE  EE+
Sbjct: 342 HEEPPPLRRRKRSDDADEVKEESSEDEAAEVESAPEKEVATKEEEAVEEAVEEE 372

HSP 4 Score: 109.0 bits (271), Expect = 1.7e-20
Identity = 111/364 (30.49%), Postives = 149/364 (40.93%), Query Frame = 1

Query: 7   PSRSVPNEKLEELVEASVREE--EEEEDTLVILVVLDKEPVGNENPEGEATNGE------ 66
           P+   P E+ E   EA+V EE  EEE +   +     +E    E    EA + E      
Sbjct: 269 PAEEAPAEE-EAPEEAAVEEEHAEEEAEEAPVEEAAAEEAPAEEADPAEAAHEEPPPLRR 328

Query: 67  -------EEVKFWEELEERVLWNGEVDKDDANKFEVEAEDKVPEEDAP--------KGLD 126
                  +EVK     +E        +K+ A K E   E+ V EE AP        + ++
Sbjct: 329 RKRSDDADEVKEESSEDEAAEVESAPEKEVATKEEEAVEEAVEEESAPAEEHAAEEEAVE 388

Query: 127 PNKPEPVLPEDVAGVPNRETPEPEAAAEAPNNPDPELAADADVPNNGAL---EVVAEDAP 186
               E   P +         P  E AAE    P  E AA+ +     A    E   E+A 
Sbjct: 389 EATEEEAAPAEEEAAEEEAAPAEEEAAEEEAAPAEEEAAEEEATEEEAHAEEEAAEEEAA 448

Query: 187 PNKDGADDAPADGAAPNKGADEVPADEERAPKEGATELPEEGAAPNEGAGKLPAPNKEV- 246
           P ++ A +  A  A      +E PA+EE A +E   E  EE A   E A +   P +E  
Sbjct: 449 PAEEEAAEEEAAPAEEEAAEEEAPAEEEAAEEEAHAE--EEAAPEEEAAEEEAGPAEEEA 508

Query: 247 --DEVPEEEAAPNKE-AEEFADEDEAPNPKELNPVEDEETPNKDEAEVAAEEGALEEDAP 306
             +E P EEAA  +  AEE A E+EAP  +E    E+EE P        AEE A EE+AP
Sbjct: 509 AEEEAPAEEAAEEEAPAEEEATEEEAPAEEEA--AEEEEAP--------AEEEAAEEEAP 568

Query: 307 VEAEAREKEKGEEVEVEKEEEPKPKEEEEGLDVGLGNGEEEEVGFEEEEEEDERDKGEGD 341
            E EA   E+  E E    EE   +EE           EEE    EEE E  E +    +
Sbjct: 569 AEEEAAHAEETAEEEAAPAEEEAVEEE--------APAEEEAAPVEEEAEHAEEEAAPAE 611

HSP 5 Score: 127.1 bits (318), Expect = 6.2e-26
Identity = 119/372 (31.99%), Postives = 161/372 (43.28%), Query Frame = 1

Query: 7   PSRSVPNEKLEELVEASVREEEEEEDTLVILVVLDKEPVGNENPEGEATNGEEEVKFWEE 66
           P+   P E+     EA+  E  EEE         ++ P   E P  EA   EEE    E 
Sbjct: 145 PAEEAPAEEEAPAEEAAEEEHAEEE------APAEEAPAEEEAPAEEAV--EEEHAEEEA 204

Query: 67  LEERVLWNGEVDKDDANKFEVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEP 126
             E      E   ++A + E  AE++ P E+AP   +    E V  E        E P  
Sbjct: 205 PAEEAPAEEEAPAEEAAE-EEHAEEEAPAEEAPAEEEAPVEEAVEEEHAEEEEAEEAPAE 264

Query: 127 EAAAEAPNN----PDPELAADADVPNNGALEVV-----AEDAPPNKDGADDAPADGAAPN 186
           EA AE  +     P  E  A+ + P   A+E       AE+AP  +  A++APA+ A P 
Sbjct: 265 EAPAEEEHAEEEAPAEEAPAEEEAPEEAAVEEEHAEEEAEEAPVEEAAAEEAPAEEADPA 324

Query: 187 KGADEVPADEERAPKEGATELPEEGAAPNEGAGKLPAPNKE--------VDEVPEEEAAP 246
           + A E P    R  +    +  +E ++ +E A    AP KE        V+E  EEE+AP
Sbjct: 325 EAAHEEPPPLRRRKRSDDADEVKEESSEDEAAEVESAPEKEVATKEEEAVEEAVEEESAP 384

Query: 247 NKEAEEFADEDEA---PNPKELNPVEDEETPNKDEAEVAAEEGALEEDAPVEAEAREKEK 306
              AEE A E+EA      +E  P E+E     +EA  A EE A EE AP E EA E+E 
Sbjct: 385 ---AEEHAAEEEAVEEATEEEAAPAEEEAA--DEEAAPAEEEAAEEEAAPAEEEAAEEEA 444

Query: 307 GEEVEVEKEEEPKPKEEEEGLDVGLGNGEEEEVGFEEEEEEDERDKGEGDDPKEKLVDPK 359
               E   EEE  P EEE           EEE   EE   E+E  + E    +E+  + +
Sbjct: 445 APAEEEAAEEEAAPAEEE---------AAEEEATEEEAHAEEEAAEEEAAPAEEEAAEEE 493

BLAST of Cucsa.120710.1 vs. NCBI nr
Match: gi|919044930|ref|XP_013405650.1| (PREDICTED: fibrous sheath CABYR-binding protein-like isoform X1 [Lingula anatina])

HSP 1 Score: 127.1 bits (318), Expect = 6.2e-26
Identity = 127/368 (34.51%), Postives = 169/368 (45.92%), Query Frame = 1

Query: 7   PSRSVPNEK---LEELVEASVREEEEEEDTLVILVVLDKEPVGNENPEGEATNGEEEVKF 66
           P+   P E+   +EE VE    EEEE E+        ++E    E P  EA   EEE   
Sbjct: 223 PAEEAPAEEEAPVEEAVEEEHAEEEEAEEAPAEEAPAEEEHAEEEAPAEEAP-AEEEAPE 282

Query: 67  WEELEERVLWNGEVDKDDANKFEVEAEDKVPEEDAPKGLDPNKPEPVLPE---DVAGVPN 126
              +EE    + E + ++A   E  AE+   EE  P      +P P+      D A    
Sbjct: 283 EAAVEEE---HAEEEAEEAPVEEAAAEEAPAEEADPAEAAHEEPPPLRRRKRSDDADEVK 342

Query: 127 RETPEPEAAAEAPNNPDPELAADADVPNNGALE---VVAEDAPPNKDGADDAPADGAAPN 186
            E+ E EAA E  + P+ E+A   +     A+E     AE+    ++  ++A  + AAP 
Sbjct: 343 EESSEDEAA-EVESAPEKEVATKEEEAVEEAVEEESAPAEEHAAEEEAVEEATEEEAAP- 402

Query: 187 KGADEVPADEERAPKEGATELPEEGAAP--NEGAGKLPAPNKEVDEVPEEEAAPNKE--A 246
             A+E  ADEE AP E   E  EE AAP   E A +  AP +E  E  EEEAAP +E  A
Sbjct: 403 --AEEEAADEEAAPAE--EEAAEEEAAPAEEEAAEEEAAPAEE--EAAEEEAAPAEEEAA 462

Query: 247 EEFADEDEAPNPKELNPVEDEETPNKDEAEVAAEEGALEEDAPVEAEAREKEKGEEVEVE 306
           EE A E+EA           EE   ++EA  A EE A EE AP E EA E+E   E E  
Sbjct: 463 EEEATEEEA---------HAEEEAAEEEAAPAEEEAAEEEAAPAEEEAAEEEAPAEEEAA 522

Query: 307 KEE---EPKPKEEEEGLDVGLGNGEEEEVGFEEEEEEDERDKGEGDDPKEKLVDPKEKPV 359
           +EE   E +   EEE  +   G  EEE      EEE    +  E + P E+    +E P 
Sbjct: 523 EEEAHAEEEAAPEEEAAEEEAGPAEEEAA----EEEAPAEEAAEEEAPAEEEATEEEAPA 565

HSP 2 Score: 114.0 bits (284), Expect = 5.4e-22
Identity = 107/357 (29.97%), Postives = 153/357 (42.86%), Query Frame = 1

Query: 7   PSRSVPNEKLEELVEASVREEEEEEDTLVILVVLDKEPVGNENPEGEATNGEEEVKFWEE 66
           P+   P E+     EA+  E  EEE         ++ P   E+ E EA    EE    EE
Sbjct: 97  PAEEAPAEEEAPAEEAAEEEHAEEEAPAEEAPAEEEAPAEEEHAEEEAP--AEEAPAEEE 156

Query: 67  LEERVLWNGEVDKDDANKFEVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEP 126
                    E  +++A   E  AE++ P E+A   ++    E   P + A  P  E    
Sbjct: 157 APAEEAAEEEHAEEEAPAEEAPAEEEAPAEEA---VEEEHAEEEAPAEEA--PAEEEAPA 216

Query: 127 EAAAEAPNNPDPELAADADVPNNGALEVVAEDAPPNKDGADDAPADGAAPNKGADEVPAD 186
           E AAE  +  +   A +A       +E   E+    ++ A++AP         A+E PA+
Sbjct: 217 EEAAEEEHAEEEAPAEEAPAEEEAPVEEAVEEEHAEEEEAEEAP---------AEEAPAE 276

Query: 187 EERAPKEG-ATELPEEGAAPNEGAGKLPAPNKEVDEVPEEEAAPNKEAEEFADEDEAPNP 246
           EE A +E  A E P E  AP E A +     +E +E P EEAA  +   E AD  EA + 
Sbjct: 277 EEHAEEEAPAEEAPAEEEAPEEAAVEEEHAEEEAEEAPVEEAAAEEAPAEEADPAEAAHE 336

Query: 247 KELNPVEDEETPNKDEAEVAAEEGALEEDAPVEAEAREKE----KGEEVEVEKEEEPKPK 306
           +   P         D+A+   EE + +E A VE+ A EKE    + E VE   EEE  P 
Sbjct: 337 E---PPPLRRRKRSDDADEVKEESSEDEAAEVES-APEKEVATKEEEAVEEAVEEESAPA 396

Query: 307 EEEEGLDVGLGNGEEEEVGFEEEEEEDERDKGEGDDPKEKLVDPKEKPVVEENGEED 359
           EE    +  +    EEE    EEE  DE    E    +E+  + +  P  EE  EE+
Sbjct: 397 EEHAAEEEAVEEATEEEAAPAEEEAADE----EAAPAEEEAAEEEAAPAEEEAAEEE 429

HSP 3 Score: 113.2 bits (282), Expect = 9.3e-22
Identity = 110/354 (31.07%), Postives = 152/354 (42.94%), Query Frame = 1

Query: 26  EEEEEEDTLVILVVLDKEPVGNENPEGEATNGEEEVKFWEELEERVLWNGEVDKDDANKF 85
           EE  +E+   +    ++ P  +E P  EA  G+EE    EE         E  +++A   
Sbjct: 42  EEAVKEEAAPVEQAAEEAPEEDEVPAEEA--GKEEAPAEEEAPAEEAVEEEHAEEEAPAE 101

Query: 86  EVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEPEAAAEAPNNPDPELAADAD 145
           E  AE++ P E+A +  + +  E    E+          E  A  EAP    P   A+ +
Sbjct: 102 EAPAEEEAPAEEAAE--EEHAEEEAPAEEAPAEEEAPAEEEHAEEEAPAEEAP---AEEE 161

Query: 146 VPNNGALEVVAEDAPPNKDGADDAPADGAAPNKGADEVPADEERAPKEGATELPEEGAAP 205
            P   A E   E+    +  A++APA+  AP + A E    EE AP E   E P E  AP
Sbjct: 162 AP---AEEAAEEEHAEEEAPAEEAPAEEEAPAEEAVEEEHAEEEAPAE---EAPAEEEAP 221

Query: 206 NEGAGKLPAPNKEV--DEVPEEEAAPNKEA--EEFADEDEAPN-PKELNPVEDEETPNKD 265
            E A +     +E   +E P EE AP +EA  EE A+E+EA   P E  P E+E      
Sbjct: 222 AEEAAEEEHAEEEAPAEEAPAEEEAPVEEAVEEEHAEEEEAEEAPAEEAPAEEEH----- 281

Query: 266 EAEVAAEEGALEEDAPVEAEAREKEKGEEVEVEKEEEPKPKEEEEGLDVGLGNGEEEEVG 325
                AEE A  E+AP E EA E+   EE   E+E E  P EE    +      +  E  
Sbjct: 282 -----AEEEAPAEEAPAEEEAPEEAAVEEEHAEEEAEEAPVEEAAAEEAPAEEADPAEAA 341

Query: 326 FEE----------------EEEEDERDKGEGDDPKEKLVDPKEKPVVEENGEED 359
            EE                +EE  E +  E +   EK V  KE+  VEE  EE+
Sbjct: 342 HEEPPPLRRRKRSDDADEVKEESSEDEAAEVESAPEKEVATKEEEAVEEAVEEE 372

HSP 4 Score: 105.1 bits (261), Expect = 2.5e-19
Identity = 97/324 (29.94%), Postives = 133/324 (41.05%), Query Frame = 1

Query: 24  VREEEEEEDTLVILVVLDKEPVGNENPEGEATNGEEEVKFWEELEERVLWNGEVDKDDAN 83
           V+EE  E++   +    +KE    E    E    EE     E   E      E  ++   
Sbjct: 337 VKEESSEDEAAEVESAPEKEVATKEEEAVEEAVEEESAPAEEHAAEE-----EAVEEATE 396

Query: 84  KFEVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEPEAAAEAPNNPDPELAAD 143
           +    AE++  +E+A    +    E   P +         P  E AAE    P  E AA+
Sbjct: 397 EEAAPAEEEAADEEAAPAEEEAAEEEAAPAEEEAAEEEAAPAEEEAAEEEAAPAEEEAAE 456

Query: 144 ADVPNNGAL---EVVAEDAPPNKDGADDAPADGAAPNKGADEVPADEERAPKEGATELPE 203
            +     A    E   E+A P ++ A +  A  A      +E PA+EE A +E   E  E
Sbjct: 457 EEATEEEAHAEEEAAEEEAAPAEEEAAEEEAAPAEEEAAEEEAPAEEEAAEEEAHAE--E 516

Query: 204 EGAAPNEGAGKLPAPNKEV---DEVPEEEAAPNKE-AEEFADEDEAPNPKELNPVEDEET 263
           E A   E A +   P +E    +E P EEAA  +  AEE A E+EAP  +E    E+EE 
Sbjct: 517 EAAPEEEAAEEEAGPAEEEAAEEEAPAEEAAEEEAPAEEEATEEEAPAEEEA--AEEEEA 576

Query: 264 PNKDEAEVAAEEGALEEDAPVEAEAREKEKGEEVEVEKEEEPKPKEEEEGLDVGLGNGEE 323
           P        AEE A EE+AP E EA   E+  E E    EE   +EE           EE
Sbjct: 577 P--------AEEEAAEEEAPAEEEAAHAEETAEEEAAPAEEEAVEEE--------APAEE 635

Query: 324 EEVGFEEEEEEDERDKGEGDDPKE 341
           E    EEE E  E +    ++  E
Sbjct: 637 EAAPVEEEAEHAEEEAAPAEEEAE 635

HSP 5 Score: 127.1 bits (318), Expect = 6.2e-26
Identity = 127/368 (34.51%), Postives = 169/368 (45.92%), Query Frame = 1

Query: 7   PSRSVPNEK---LEELVEASVREEEEEEDTLVILVVLDKEPVGNENPEGEATNGEEEVKF 66
           P+   P E+   +EE VE    EEEE E+        ++E    E P  EA   EEE   
Sbjct: 123 PAEEAPAEEEAPVEEAVEEEHAEEEEAEEAPAEEAPAEEEHAEEEAPAEEAP-AEEEAPE 182

Query: 67  WEELEERVLWNGEVDKDDANKFEVEAEDKVPEEDAPKGLDPNKPEPVLPE---DVAGVPN 126
              +EE    + E + ++A   E  AE+   EE  P      +P P+      D A    
Sbjct: 183 EAAVEEE---HAEEEAEEAPVEEAAAEEAPAEEADPAEAAHEEPPPLRRRKRSDDADEVK 242

Query: 127 RETPEPEAAAEAPNNPDPELAADADVPNNGALE---VVAEDAPPNKDGADDAPADGAAPN 186
            E+ E EAA E  + P+ E+A   +     A+E     AE+    ++  ++A  + AAP 
Sbjct: 243 EESSEDEAA-EVESAPEKEVATKEEEAVEEAVEEESAPAEEHAAEEEAVEEATEEEAAP- 302

Query: 187 KGADEVPADEERAPKEGATELPEEGAAP--NEGAGKLPAPNKEVDEVPEEEAAPNKE--A 246
             A+E  ADEE AP E   E  EE AAP   E A +  AP +E  E  EEEAAP +E  A
Sbjct: 303 --AEEEAADEEAAPAE--EEAAEEEAAPAEEEAAEEEAAPAEE--EAAEEEAAPAEEEAA 362

Query: 247 EEFADEDEAPNPKELNPVEDEETPNKDEAEVAAEEGALEEDAPVEAEAREKEKGEEVEVE 306
           EE A E+EA           EE   ++EA  A EE A EE AP E EA E+E   E E  
Sbjct: 363 EEEATEEEA---------HAEEEAAEEEAAPAEEEAAEEEAAPAEEEAAEEEAPAEEEAA 422

Query: 307 KEE---EPKPKEEEEGLDVGLGNGEEEEVGFEEEEEEDERDKGEGDDPKEKLVDPKEKPV 359
           +EE   E +   EEE  +   G  EEE      EEE    +  E + P E+    +E P 
Sbjct: 423 EEEAHAEEEAAPEEEAAEEEAGPAEEEAA----EEEAPAEEAAEEEAPAEEEATEEEAPA 465

BLAST of Cucsa.120710.1 vs. NCBI nr
Match: gi|919044934|ref|XP_013405652.1| (PREDICTED: fibrous sheath CABYR-binding protein-like isoform X3 [Lingula anatina])

HSP 1 Score: 124.8 bits (312), Expect = 3.1e-25
Identity = 115/359 (32.03%), Postives = 157/359 (43.73%), Query Frame = 1

Query: 20  VEASVREEEEEEDTLVILVVLDKEPVGNENPEGEATNGEEEVKFWEELEERVLWNGEVDK 79
           VE +  E  EE++        ++ P   E P  EA   EEE    E   E      E   
Sbjct: 52  VEQAAEEAPEEDEVPAEEAGKEEAPAEEEAPAEEAV--EEEHAEEEAPAEEAPAEEEAPA 111

Query: 80  DDANKFEVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEPEAAAEAPNN---- 139
           ++A + E  AE++ P E+AP   +    E V  E        E P  EA AE  +     
Sbjct: 112 EEAAE-EEHAEEEAPAEEAPAEEEAPVEEAVEEEHAEEEEAEEAPAEEAPAEEEHAEEEA 171

Query: 140 PDPELAADADVPNNGALEVV-----AEDAPPNKDGADDAPADGAAPNKGADEVPADEERA 199
           P  E  A+ + P   A+E       AE+AP  +  A++APA+ A P + A E P    R 
Sbjct: 172 PAEEAPAEEEAPEEAAVEEEHAEEEAEEAPVEEAAAEEAPAEEADPAEAAHEEPPPLRRR 231

Query: 200 PKEGATELPEEGAAPNEGAGKLPAPNKE--------VDEVPEEEAAPNKEAEEFADEDEA 259
            +    +  +E ++ +E A    AP KE        V+E  EEE+AP   AEE A E+EA
Sbjct: 232 KRSDDADEVKEESSEDEAAEVESAPEKEVATKEEEAVEEAVEEESAP---AEEHAAEEEA 291

Query: 260 ---PNPKELNPVEDEETPNKDEAEVAAEEGALEEDAPVEAEAREKEKGEEVEVEKEEEPK 319
                 +E  P E+E     +EA  A EE A EE AP E EA E+E     E   EEE  
Sbjct: 292 VEEATEEEAAPAEEEAA--DEEAAPAEEEAAEEEAAPAEEEAAEEEAAPAEEEAAEEEAA 351

Query: 320 PKEEEEGLDVGLGNGEEEEVGFEEEEEEDERDKGEGDDPKEKLVDPKEKPVVEENGEED 359
           P EEE           EEE   EE   E+E  + E    +E+  + +  P  EE  EE+
Sbjct: 352 PAEEE---------AAEEEATEEEAHAEEEAAEEEAAPAEEEAAEEEAAPAEEEAAEEE 393

HSP 2 Score: 108.6 bits (270), Expect = 2.3e-20
Identity = 101/346 (29.19%), Postives = 147/346 (42.49%), Query Frame = 1

Query: 34  LVILVVLDKEPVGNENPEGEATNG----------------EEEVKFWEELEERVLWNGEV 93
           ++  V++   P G   P  + T G                +EE    E+  E      EV
Sbjct: 6   ILCFVLVASFPYGKAAPVPDVTEGSEDDAKSAEVSHEEAVKEEAAPVEQAAEEAPEEDEV 65

Query: 94  DKDDANKFEVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEPEAAAEAPNNPD 153
             ++A K E  AE++ P E+A   ++    E   P + A  P  E    E AAE  +  +
Sbjct: 66  PAEEAGKEEAPAEEEAPAEEA---VEEEHAEEEAPAEEA--PAEEEAPAEEAAEEEHAEE 125

Query: 154 PELAADADVPNNGALEVVAEDAPPNKDGADDAPADGAAPNKGADEVPADEERAPKEG-AT 213
              A +A       +E   E+    ++ A++AP         A+E PA+EE A +E  A 
Sbjct: 126 EAPAEEAPAEEEAPVEEAVEEEHAEEEEAEEAP---------AEEAPAEEEHAEEEAPAE 185

Query: 214 ELPEEGAAPNEGAGKLPAPNKEVDEVPEEEAAPNKEAEEFADEDEAPNPKELNPVEDEET 273
           E P E  AP E A +     +E +E P EEAA  +   E AD  EA + +   P      
Sbjct: 186 EAPAEEEAPEEAAVEEEHAEEEAEEAPVEEAAAEEAPAEEADPAEAAHEE---PPPLRRR 245

Query: 274 PNKDEAEVAAEEGALEEDAPVEAEAREKE----KGEEVEVEKEEEPKPKEEEEGLDVGLG 333
              D+A+   EE + +E A VE+ A EKE    + E VE   EEE  P EE    +  + 
Sbjct: 246 KRSDDADEVKEESSEDEAAEVES-APEKEVATKEEEAVEEAVEEESAPAEEHAAEEEAVE 305

Query: 334 NGEEEEVGFEEEEEEDERDKGEGDDPKEKLVDPKEKPVVEENGEED 359
              EEE    EEE  DE    E    +E+  + +  P  EE  EE+
Sbjct: 306 EATEEEAAPAEEEAADE----EAAPAEEEAAEEEAAPAEEEAAEEE 329

HSP 3 Score: 105.1 bits (261), Expect = 2.5e-19
Identity = 97/324 (29.94%), Postives = 133/324 (41.05%), Query Frame = 1

Query: 24  VREEEEEEDTLVILVVLDKEPVGNENPEGEATNGEEEVKFWEELEERVLWNGEVDKDDAN 83
           V+EE  E++   +    +KE    E    E    EE     E   E      E  ++   
Sbjct: 237 VKEESSEDEAAEVESAPEKEVATKEEEAVEEAVEEESAPAEEHAAEE-----EAVEEATE 296

Query: 84  KFEVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEPEAAAEAPNNPDPELAAD 143
           +    AE++  +E+A    +    E   P +         P  E AAE    P  E AA+
Sbjct: 297 EEAAPAEEEAADEEAAPAEEEAAEEEAAPAEEEAAEEEAAPAEEEAAEEEAAPAEEEAAE 356

Query: 144 ADVPNNGAL---EVVAEDAPPNKDGADDAPADGAAPNKGADEVPADEERAPKEGATELPE 203
            +     A    E   E+A P ++ A +  A  A      +E PA+EE A +E   E  E
Sbjct: 357 EEATEEEAHAEEEAAEEEAAPAEEEAAEEEAAPAEEEAAEEEAPAEEEAAEEEAHAE--E 416

Query: 204 EGAAPNEGAGKLPAPNKEV---DEVPEEEAAPNKE-AEEFADEDEAPNPKELNPVEDEET 263
           E A   E A +   P +E    +E P EEAA  +  AEE A E+EAP  +E    E+EE 
Sbjct: 417 EAAPEEEAAEEEAGPAEEEAAEEEAPAEEAAEEEAPAEEEATEEEAPAEEEA--AEEEEA 476

Query: 264 PNKDEAEVAAEEGALEEDAPVEAEAREKEKGEEVEVEKEEEPKPKEEEEGLDVGLGNGEE 323
           P        AEE A EE+AP E EA   E+  E E    EE   +EE           EE
Sbjct: 477 P--------AEEEAAEEEAPAEEEAAHAEETAEEEAAPAEEEAVEEE--------APAEE 535

Query: 324 EEVGFEEEEEEDERDKGEGDDPKE 341
           E    EEE E  E +    ++  E
Sbjct: 537 EAAPVEEEAEHAEEEAAPAEEEAE 535

HSP 4 Score: 124.4 bits (311), Expect = 4.0e-25
Identity = 119/352 (33.81%), Postives = 166/352 (47.16%), Query Frame = 1

Query: 11  VPNEKLEELVEASVREEEEEEDTLVILVVLDKEPVGNENPEGEATNGEEEVKFWEELEER 70
           VP E++E   EA+  EEEEE +  V            E PEGE     EE +  EE E+ 
Sbjct: 15  VPEEEVEPEEEAAPEEEEEEPEEEV------------EEPEGEDEEEPEEEEEPEEEEDE 74

Query: 71  VLWNGEVDKDDANKFEVEAEDKVPEEDAPKGLDPNKPEPVLPEDVAGVPNRETPEPEAAA 130
                E ++++    E   E++V EE  P+G+D   PE    E+     + E PE E   
Sbjct: 75  -----EPEEEEEPGEEAAPEEEVEEE--PEGVDEEDPEV---EEAPEGEDEEEPEEEEEL 134

Query: 131 EAPNNPDPELAADADVPNNGALEVVAEDAPPNKDGADDAPADGAAPNKGADEVPADEERA 190
           E    P  E   + +       E  AE+  P  +G ++ P +   P    +EV  +EE A
Sbjct: 135 EEEEAPVEE--EEEEPEGEDEEEPEAEEEVPEGEGEEE-PEEEEVPE---EEVEPEEEAA 194

Query: 191 PKEGATELPEEGAAPNEGAGKLPAPNKEVDEVPEEEAA-PNKEAEEFADEDEAPNPKELN 250
           P+E   E PEE   P E   +   P  EV+E PEEE   P+ E EE   E+E    +E+ 
Sbjct: 195 PEEEEEE-PEEEEEPEEEEQE---PEGEVEEEPEEEEQEPDGEDEEEPAEEEVELDEEIE 254

Query: 251 PVEDEETPNKDEAEVAAEEGALEEDAPVEAEAREKEKGEEVEVEK----EEEPKPKEEEE 310
             EDEE P ++EA    EE  LEED   E E  EK + EE   ++    EEE +P+EE E
Sbjct: 255 --EDEEEP-EEEAAPEEEEEELEEDGDGEPEEEEKPEDEEKTDDEPEVIEEEDEPEEETE 314

Query: 311 GLDVGLGNGEEEEVGFEEEEEEDERDKGEGDDPKEKLVDPKEKPVVEENGEE 358
            ++      EEEE   EE EEE E +  E ++ +E   D K +   EE  +E
Sbjct: 315 DVE------EEEEPEVEELEEEPEEEVEELENKEETEEDDKPEKEEEEESKE 325