Cucsa.107260.1 (mRNA) Cucumber (Gy14) v1

BLAST of Cucsa.107260.1 vs. Swiss-Prot
Match: XLG1_ARATH (Extra-large guanine nucleotide-binding protein 1 OS=Arabidopsis thaliana GN=XLG1 PE=1 SV=2)

HSP 1 Score: 67.0 bits (162), Expect = 1.2e-09
Identity = 39/112 (34.82%), Postives = 58/112 (51.79%), Query Frame = 1

Query: 764 VSVNGQPLPAHIVKKAEKHAGP---ILPGDYWYDYQAGFWGVMGHPCLGIIPPFIDEFTY 823
           V VNGQPL    +   +  + P   + PGDYWYD  +G WG  G     II P ++    
Sbjct: 317 VYVNGQPLYPEELVTLQTCSNPPKKLKPGDYWYDKVSGLWGKEGEKPYQIISPHLN-VGG 376

Query: 824 PMSRNCAAGNTGIFVNGRELHKRDLELLSSRGLPTTTNKLYRIDISGRVIDE 873
           P+S   + GNT +F+NGRE+ K +L +L   G+    N  + ++  G   +E
Sbjct: 377 PISPEASNGNTQVFINGREITKVELRMLQLAGVQCAGNPHFWVNEDGSYQEE 427

BLAST of Cucsa.107260.1 vs. Swiss-Prot
Match: Y5519_ARATH (Uncharacterized protein At5g05190 OS=Arabidopsis thaliana GN=Y-1 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.0e-08
Identity = 42/140 (30.00%), Postives = 69/140 (49.29%), Query Frame = 1

Query: 3   TAQKVRVVRCPRCQNLLPEPSGLSVYQCGGCGIVLKAKSK-VLFNEKRDFGSSEKYENFS 62
           T QK+R+VRCP+C  +L E   + VYQCGGC  +L+AK + +  +     G +E+ +   
Sbjct: 5   TGQKIRLVRCPKCLKILQEDEDVPVYQCGGCSAILQAKRRNIAPSSTPSAGETERAQANE 64

Query: 63  EQGGSSLGGVSDSERGSPSMEPTVNERVMRSRRTVFSNSPIRTSDREDIDDYERKIGKET 122
            Q       VS S  G  ++ P+   R +        N+ + ++++E +DD E   G  T
Sbjct: 65  PQSVPETNNVSSSS-GQDTVLPSSPGRSVDQEYEKGRNASMESTEKE-LDDLELSNGDGT 124

Query: 123 KGVWPMQ-RLGDKEIGSVEE 141
             +   +  LGD E    E+
Sbjct: 125 NEIQEQECSLGDSEKNERED 142

HSP 2 Score: 47.8 bits (112), Expect = 7.5e-04
Identity = 27/91 (29.67%), Postives = 43/91 (47.25%), Query Frame = 1

Query: 501 AGGAPFIVCSSCLELLKLPRKLYRLEVDWQKLQCGACSVVIIVKVENRKLV-------IS 560
           AGGAPF+ C SC E L+LP      +     L+CG C+ V+   +++R  +       I+
Sbjct: 494 AGGAPFVSCYSCSENLQLPVDFLIFKRKHHLLRCGTCTTVLRFSLQSRNHLVPAVTHDIN 553

Query: 561 VPAETKPTEVSPNDSSPKSVVNATSSIESSD 585
               +  T  SP D +P       SS++  +
Sbjct: 554 ANRNSNSTSESPIDKAPSKPEKLRSSVQDEE 584

BLAST of Cucsa.107260.1 vs. Swiss-Prot
Match: XLG3_ARATH (Extra-large guanine nucleotide-binding protein 3 OS=Arabidopsis thaliana GN=XLG3 PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 9.5e-07
Identity = 37/125 (29.60%), Postives = 61/125 (48.80%), Query Frame = 1

Query: 766 VNGQPL-PAHIVKKAEKHAGP--ILPGDYWYDYQAGFWGVMGHPCLGIIPPFIDEFTYPM 825
           VNG PL P  +         P  + PG YWYD ++G WG  G     +I   ++ FT  +
Sbjct: 269 VNGYPLKPEEMADLLNCLLPPQKLKPGRYWYDKESGLWGKEGEKPDRVISSNLN-FTGKL 328

Query: 826 SRNCAAGNTGIFVNGRELHKRDLELLSSRGLPTTTNKLYRIDISGRVIDEDSGKVLYNLG 885
           S + + GNT +++NGRE+ K +L +L    +    +  + +   GR  +E    +  N+ 
Sbjct: 329 SPDASNGNTEVYINGREITKLELRILKLANVQCPRDTHFWVYDDGRYEEEGQNNIRGNIW 388

Query: 886 KLAPT 888
           + A T
Sbjct: 389 EKAST 392

BLAST of Cucsa.107260.1 vs. Swiss-Prot
Match: XLG2_ARATH (Extra-large guanine nucleotide-binding protein 2 OS=Arabidopsis thaliana GN=XLG2 PE=1 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 2.8e-06
Identity = 36/121 (29.75%), Postives = 57/121 (47.11%), Query Frame = 1

Query: 764 VSVNGQPLPAHIVKKAEKHAGP---ILPGDYWYDYQAGFWGVMGHPCLGIIPPFIDEFTY 823
           + VN +PL    +   +    P   + PG YWYD  AG+WG +G     II P      Y
Sbjct: 306 IIVNDKPLSEDELYTLQTCPNPPKKLKPGHYWYDKVAGYWGKIGEKPSQIISPNNSIGGY 365

Query: 824 PMSRNCAAGNTGIFVNGRELHKRDLELLSSRGLPTTTNKLYRIDISGRVIDEDSGKVLYN 882
            +S   + G+T I++NGRE+ K +L +L   G+       + +D  G   +E     + N
Sbjct: 366 -ISEKVSNGDTEIYINGREITKPELTMLKWAGVQCEGKPHFWVDSDGSYREEGQKHPIGN 425

BLAST of Cucsa.107260.1 vs. Swiss-Prot
Match: ESF1_RAT (ESF1 homolog OS=Rattus norvegicus GN=Esf1 PE=1 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 4.7e-06
Identity = 51/187 (27.27%), Postives = 86/187 (45.99%), Query Frame = 1

Query: 557 ETKPTEVSPNDSSPKSVVNATSSIESSDNSSLKVIDTDHNKPSDDQDSNCAKPQEE---- 616
           ETK T+    DS  K+ +N +  I+   NS           P D ++S  ++ ++     
Sbjct: 117 ETKKTD--QKDSINKNDLNNSERIQKMKNSHKSPKIDSEVSPKDSEESLQSRKKKRDTTD 176

Query: 617 --VTSSPISSKEKESPTINCDPKNLSDSDDLPLKDTPSVISSVENSDNPSHDKPSEHREG 676
             V +SP      + P+ +   K+ + S     ++  +VI + +N+    H++  E    
Sbjct: 177 LSVEASPKGKLRTKDPSTSAMVKSSTVSGSKAKREKQAVIMAKDNAGRMLHEEAPE---- 236

Query: 677 TEDKQKVMVDDVTEPSELDVSFDDYANIHVSHDSVEINKEEEEEEGEEGEEGEEDQSKIK 736
            ED          E SE +++ DD A    S D  E   EEEEE+GEE EE EE++   +
Sbjct: 237 -EDSDSASELGRDEESEGEITSDDRA----SADDDENEDEEEEEDGEEEEEEEEEED--E 290

Query: 737 SNQESET 738
           S+ ES++
Sbjct: 297 SDDESDS 290

BLAST of Cucsa.107260.1 vs. TrEMBL
Match: A0A0A0KUV2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G051420 PE=4 SV=1)

HSP 1 Score: 1697.2 bits (4394), Expect = 0.0e+00
Identity = 824/830 (99.28%), Postives = 824/830 (99.28%), Query Frame = 1

Query: 81  MEPTVNERVMRSRRTVFSNSPIRTSDREDIDDYERKIGKETKGVWPMQRLGDKEIGSVEE 140
           MEPTVNERVMRSRRTVFSNSPIRTSDREDIDDYERKIGKETKGVWPMQRLGDKEIGSVEE
Sbjct: 1   MEPTVNERVMRSRRTVFSNSPIRTSDREDIDDYERKIGKETKGVWPMQRLGDKEIGSVEE 60

Query: 141 THSQFSEQRIENWVRRYNIEQDMNIYDSDSPSKAPYRNPTGAARTRATFEHHRVERDAFA 200
           THSQFSEQRIENWVRRYNIEQDMNIYDSDSPSKAPYRNPTGAARTRATFEHHRVERDAFA
Sbjct: 61  THSQFSEQRIENWVRRYNIEQDMNIYDSDSPSKAPYRNPTGAARTRATFEHHRVERDAFA 120

Query: 201 GYSGNSMAGAHGKGAPNFRYPGDRPSSSNLNVYYGHPEPNRNYEGPIEGFDPNRAELLRR 260
           GYSGNSMAGAHGKGAPNFRYPGDRPSSSNLNVYYGHPEPNRNYEGPIEGFDPNRAELLRR
Sbjct: 121 GYSGNSMAGAHGKGAPNFRYPGDRPSSSNLNVYYGHPEPNRNYEGPIEGFDPNRAELLRR 180

Query: 261 LDELKAEIIKSCDVGDRPRVVADRAPVDPYYSRAGYNVPMRSSTKNPQHNHDPQYFGRGS 320
           LDELKAEIIKSCDVGDRPRVVADRAPVDPYYSRAGYNVPMRSSTKNPQHNHDPQYFGRGS
Sbjct: 181 LDELKAEIIKSCDVGDRPRVVADRAPVDPYYSRAGYNVPMRSSTKNPQHNHDPQYFGRGS 240

Query: 321 GTFPATGHHQRNGEDFLHPPRHVVKDMPLYEDEFQEQMIRKTNHQPAHQYPPRQHYPESV 380
           GTFPATGHHQRNGEDFLHPPRHVVKDMPLYEDEFQEQMIRKTNHQPAHQYPPRQHYPESV
Sbjct: 241 GTFPATGHHQRNGEDFLHPPRHVVKDMPLYEDEFQEQMIRKTNHQPAHQYPPRQHYPESV 300

Query: 381 MDFKQDPLSPLHDEDVFFHHPACSCSQCGKRNRQGPPNSPASNVSNPKEPIKSSTYHNET 440
           MDFKQDPLSPLHDEDVFFHHPACSCSQCGKRNRQGPPNSPASNVSNPKEPIKSSTYHNET
Sbjct: 301 MDFKQDPLSPLHDEDVFFHHPACSCSQCGKRNRQGPPNSPASNVSNPKEPIKSSTYHNET 360

Query: 441 PVSVGLMASNLPCAGRFPSQDTLPHSRQPSELDSEIDGFGLVQPRTAAVFQRNGKSRDAI 500
           PVSVGLMASNLPCAGRFPSQDTLPHSRQPSELDSEIDGFGLVQPRTAAVFQRNGKSRDAI
Sbjct: 361 PVSVGLMASNLPCAGRFPSQDTLPHSRQPSELDSEIDGFGLVQPRTAAVFQRNGKSRDAI 420

Query: 501 AGGAPFIVCSSCLELLKLPRKLYRLEVDWQKLQCGACSVVIIVKVENRKLVISVPAETKP 560
           AGGAPFIVCSSCLELLKLPRKLYRLEVDWQKLQCGACSVVIIVKVENRKLVISVPAETKP
Sbjct: 421 AGGAPFIVCSSCLELLKLPRKLYRLEVDWQKLQCGACSVVIIVKVENRKLVISVPAETKP 480

Query: 561 TEVSPNDSSPKSVVNATSSIESSDNSSLKVIDTDHNKPSDDQDSNCAKPQEEVTSSPISS 620
           TEVSPNDSSPKSVVNATSSIESSDNSSLKVIDTDHNKPSDDQDSNCAKPQEEVTSSPISS
Sbjct: 481 TEVSPNDSSPKSVVNATSSIESSDNSSLKVIDTDHNKPSDDQDSNCAKPQEEVTSSPISS 540

Query: 621 KEKESPTINCDPKNLSDSDDLPLKDTPSVISSVENSDNPSHDKPSEHREGTEDKQKVMVD 680
           KEKESPTINCDPKNLSDSDDLPLKDTPSVISSVENSDNPSHDKPSEHREGTEDKQKVMVD
Sbjct: 541 KEKESPTINCDPKNLSDSDDLPLKDTPSVISSVENSDNPSHDKPSEHREGTEDKQKVMVD 600

Query: 681 DVTEPSELDVSFDDYANIHVSHDSVEINKEEEE------EEGEEGEEGEEDQSKIKSNQE 740
           DVTEPSELDVSFDDYANIHVSHDSVEINKEEEE      EEGEEGEEGEEDQSKIKSNQE
Sbjct: 601 DVTEPSELDVSFDDYANIHVSHDSVEINKEEEEEEGEEGEEGEEGEEGEEDQSKIKSNQE 660

Query: 741 SETFFVGLSRNNLRDFSRSSEITDNGRPTVSVNGQPLPAHIVKKAEKHAGPILPGDYWYD 800
           SETFFVGLSRNNLRDFSRSSEITDNGRPTVSVNGQPLPAHIVKKAEKHAGPILPGDYWYD
Sbjct: 661 SETFFVGLSRNNLRDFSRSSEITDNGRPTVSVNGQPLPAHIVKKAEKHAGPILPGDYWYD 720

Query: 801 YQAGFWGVMGHPCLGIIPPFIDEFTYPMSRNCAAGNTGIFVNGRELHKRDLELLSSRGLP 860
           YQAGFWGVMGHPCLGIIPPFIDEFTYPMSRNCAAGNTGIFVNGRELHKRDLELLSSRGLP
Sbjct: 721 YQAGFWGVMGHPCLGIIPPFIDEFTYPMSRNCAAGNTGIFVNGRELHKRDLELLSSRGLP 780

Query: 861 TTTNKLYRIDISGRVIDEDSGKVLYNLGKLAPTIEKVKHGFGMKVPRTLK 905
           TTTNKLYRIDISGRVIDEDSGKVLYNLGKLAPTIEKVKHGFGMKVPRTLK
Sbjct: 781 TTTNKLYRIDISGRVIDEDSGKVLYNLGKLAPTIEKVKHGFGMKVPRTLK 830

BLAST of Cucsa.107260.1 vs. TrEMBL
Match: F6HUS3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_02s0025g01190 PE=4 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 9.5e-163
Identity = 366/955 (38.32%), Postives = 515/955 (53.93%), Query Frame = 1

Query: 1   MATAQKVRVVRCPRCQNLLPEPSGLSVYQCGGCGIVLKAKSKVLFN----EKRDF----G 60
           MA   KVRVVRCP+C+NLLPE     VYQCGGCG VL+AK K   N    EK D     G
Sbjct: 1   MAEGSKVRVVRCPKCENLLPELPDYPVYQCGGCGAVLRAKKKAPSNDALSEKSDDENGRG 60

Query: 61  SSEKYENFSEQGGSSLGGVSDSERGSPSME-PTVNERVMRSR-RTVFSNSPIRTSDREDI 120
            SEK E+ SE+G  SLG  S++E+ S  +E     E V+  +   + S+S  RT +RE +
Sbjct: 61  VSEKLESLSEKGAVSLGSCSETEKESDGVEHGRKKESVLGEKPENLISSSVSRTENREIV 120

Query: 121 DDYERKIGKETKGVWPMQRLGDKEIGSVEETHSQFSEQRIENWVRRYNIEQDMNIYDSDS 180
           + ++  + +E  G+  + R     +G            R E    R N  +      S  
Sbjct: 121 NGHDMNMKREAMGL-RVDRSNGWGVG------------REELGAFRRNSREQGRFSTSPY 180

Query: 181 PSKAPYRNPTGAARTRATFEHHRVERDAFAGYSGNSMAGAHGKGAPNFRYPGDRPSSSNL 240
           P + P     G                +F GY G  M      G PN             
Sbjct: 181 PDEGPSNFHPG----------------SFYGY-GQPMKHHDNIGGPNR------------ 240

Query: 241 NVYYGHPEPNRNYEGPIEGFDPNRAELLRRLDELKAEIIKSCDVGDRPRVVADRAPVDPY 300
                            E  + +R ELLR+LDELK ++ +SC V D+PR           
Sbjct: 241 ----------------AENLEQDRVELLRKLDELKDQLSRSCAVEDKPR----------- 300

Query: 301 YSRAGYNVPMRSSTKNPQHNHDPQYFGRGSGTFPATGHHQRNGEDFLHPPRHVVKDMPLY 360
                                 P YF  G G+ P    H  + + F  P RHV  ++P Y
Sbjct: 301 --------------------ERPPYFSHGHGSVPFMNGHDMDMQSFYPPARHVPDEIPGY 360

Query: 361 EDEFQEQMIRK-TNHQPAHQY---PPRQHYPESVMDFKQDPLSPLHDEDVFFHHPACSCS 420
           ED FQ Q++R+ T HQP HQY   P  +++    M++ QDP +  H  + FFH PACSC 
Sbjct: 361 EDPFQPQVLRRATTHQPPHQYLQRPYHEYFSGRYMEYNQDPFASYH--ETFFHQPACSCV 420

Query: 421 QCGKRNRQGPPNSPASNVSNPKEPIKSST---YHNETPVSVGLMASNLPCAGRFPS--QD 480
           +C  +N Q PP  P +     + PI+S     YH+  P + G    N P     PS  +D
Sbjct: 421 RCCNKNWQVPPQVPPTTFGKRRFPIESKNPNFYHHVNPPTFGSRGYN-PRGSNPPSHPRD 480

Query: 481 TLPHSRQPSELDSEIDGFGLVQPRTAAVFQRNGKSRDAIAGGAPFIVCSSCLELLKLPRK 540
             PH+R PS++DS+I GF   +PR   V   N +    I GGAPFI C +C ELLK+PRK
Sbjct: 481 PQPHTRWPSDIDSDIGGFSQYRPRRVVVAHGNRRLCHPIVGGAPFITCYNCFELLKVPRK 540

Query: 541 LYRLEVDWQKLQCGACSVVIIVKVENRKLVISVPAETKPTEVSPNDSSPKSV-------- 600
              ++ + +KLQCGACS V  ++VEN+K+++SVP + K      +D S + +        
Sbjct: 541 FMLMDKNQRKLQCGACSCVNFLEVENKKVIVSVPTQMKRRSPDADDGSCEVLDHYHRSSH 600

Query: 601 ----VNATSSIE-SSDNSSLKVIDTDHNKPSDD--QDSNCAKPQEEVTSSPISSKEKESP 660
               V  T+S +  +   + + IDT+ N PS D       AK Q  ++SSP S++++ESP
Sbjct: 601 AHLNVGGTNSDDFDTSGYNFQSIDTEPNLPSKDCILIGEAAKRQGLLSSSPSSTEDEESP 660

Query: 661 TINCDPKNLSDSDDLPLKD--TPSVISS--VENSDNPSHDKPSEHREGTE----DKQKVM 720
                 +++S S +LPLK+  +P +++S   EN D  S+   S H +G +    D++KV+
Sbjct: 661 DSMIGQRDISSSAELPLKEDVSPPLLASPLQENFDYSSNHAMSRHGKGNKSKRTDEEKVI 720

Query: 721 ----------VDDVTEPSELDVSFDDYANIHVSHDSVEINKEEEEEEGEEGEEGEEDQSK 780
                     V D    +E++V F++Y N  +S +SVE++K+E+               +
Sbjct: 721 LNKATSRQNSVKDAAVATEMEVCFNEYLNTGLSQESVEVSKDED---------------R 780

Query: 781 IKSNQESETFFVGLSRNNLRDFSRSSEITDNGRPTVSVNGQPLPAHIVKKAEKHAGPILP 840
            K+N+ S++FF GL + + RDF+RS+   DN +P VSVNGQP+P   VKKAEK AGP+ P
Sbjct: 781 PKNNKGSDSFFAGLIKKSFRDFTRSNHSMDNSKPKVSVNGQPIPERAVKKAEKQAGPVHP 840

Query: 841 GDYWYDYQAGFWGVMGHPCLGIIPPFIDEFTYPMSRNCAAGNTGIFVNGRELHKRDLELL 900
           G+YWYD++AGFWGVMG PCLGIIPPFI+EF   M  +C++GNTG+FVNGRELH++DL+LL
Sbjct: 841 GEYWYDFRAGFWGVMGQPCLGIIPPFIEEFNCSMPEDCSSGNTGVFVNGRELHQKDLDLL 848

Query: 901 SSRGLPTTTNKLYRIDISGRVIDEDSGKVLYNLGKLAPTIEKVKHGFGMKVPRTL 904
           +SRGLPTT +K Y I+ISGRV+DED+G+ L +LGKLAPT+EK+K GFGMKVPR +
Sbjct: 901 ASRGLPTTRDKSYIIEISGRVLDEDTGEELDSLGKLAPTVEKLKCGFGMKVPRAV 848

BLAST of Cucsa.107260.1 vs. TrEMBL
Match: W9RY80_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_012064 PE=4 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 2.2e-151
Identity = 355/968 (36.67%), Postives = 511/968 (52.79%), Query Frame = 1

Query: 1   MATAQKVRVVRCPRCQNLLPEPSGLSVYQCGGCGIVLKAKSKVLFN--------EKRDFG 60
           MA   KVR+VRCP+C NLLPE    SVYQCGGCG +L+AK +   N        E++  G
Sbjct: 1   MAEGSKVRLVRCPKCDNLLPELPDYSVYQCGGCGAILRAKKRYADNDMLSEKSDEEKGGG 60

Query: 61  SSEKYENFSEQGGSSLGGVSDSERGSPSMEPTVNERVMRSRRTVFSNSPI-------RTS 120
            SEK E   E+G  +    S+SE     ME + NE V R  R               RT 
Sbjct: 61  DSEKLEKLVEKGSDNSNFASESE-----MEDSGNEHVRRKERAFVEKKANFIRGGLQRTE 120

Query: 121 DREDIDDYERKIGKETKGVWPMQRLGDKEIGSVEETHSQFSEQRIENWVRRYNIEQDMNI 180
            ++ +   +  + +E K +   Q + ++E GSV+  +++ SE +++         +DM+ 
Sbjct: 121 KKQVLVGKDTSVSEEYKDLRLDQCVEEREYGSVDR-YTRLSEHQMDPRTCGTGRYRDMSR 180

Query: 181 YDSDSPS----KAPYRNPTGAARTRATFEHHRVERDA-FAGYSGNSMAGAHGKGAPNFRY 240
            +S + S           TG+ R+RA      VER +   G  GN+      + A  F Y
Sbjct: 181 SESVNSSLEEISTQVNRFTGSLRSRAGMGWSGVERGSRLEGLYGNNSVSDQER-ARTFGY 240

Query: 241 PGDRPSSSNLNVYYGHPEP---NRNYEGP--IEGFDPNRAELLRRLDELKAEIIKSCDVG 300
           P +  S+ +  ++  H       ++ + P  ++  +  RAE+LR+LDELK ++ +S  V 
Sbjct: 241 PDEGTSNYDPYLFRDHERQIISGQHVKVPDGVKNLEQERAEILRKLDELKEQLSRSYHVA 300

Query: 301 DRPRVVADRAPVD-----PYYSRAGYNVPMRSSTKNPQHNHDPQYFGRGSGTFPATGHHQ 360
           D+P+   +R P D     PY  +  YNV M+    + Q    P Y     G+ P T HH 
Sbjct: 301 DKPK---EREPTDRTLPYPYGIQVPYNVSMQPPALDKQVPRPPHY-NYSHGSVPHTNHHH 360

Query: 361 RNGEDFLHPPRHVVKDMPLYEDEFQEQMIRKTNHQPAHQYP---PRQHYPESVMDFKQDP 420
            +  +   P +H +  +P Y+D FQ Q+ RK +  P HQY    P ++Y      F    
Sbjct: 361 VDMHNLYPPRKHSLNKIPEYDDPFQPQLTRKPHRLPPHQYQHVSPHEYYSGQYRTFDLVE 420

Query: 421 LSPLHDEDVFFHHPACSCSQCGKRNRQGPPNSPASNVSNPKEPIKSSTYHNETPVSVGLM 480
               +  + F H P C+C  C  +N Q PP+ P     + K PI  + Y +  PV  G  
Sbjct: 421 SIASYPHETFSHAPTCACLSCYNQNLQVPPSVP-----HTKAPINPNFYRHGDPVGFGPQ 480

Query: 481 ASNLPCAGRFPSQDTLP---HSRQPSELDSEIDGFGLVQPRTAAVFQRNGKSRDAIAGGA 540
           +         P  ++L    H+R P +L+SE + +G  QPR  A   + G+    IAGGA
Sbjct: 481 SC--------PPSESLHQHLHTRWPGDLESEHNSYG--QPRRVAATCKTGRLYHPIAGGA 540

Query: 541 PFIVCSSCLELLKLPRKLYRLEVDWQKLQCGACSVVIIVKVENRKLVISVPAETKPTEVS 600
           PFI C  C ELLKLPRKL     + Q+L+CGACS VI++++EN+KL++S P+E K     
Sbjct: 541 PFITCHKCFELLKLPRKLGISGSNEQRLRCGACSAVILLEMENKKLIMSDPSELKRLSAE 600

Query: 601 PNDSSPKSVVNATSSIESSDNSSLKVIDTDHNKPS--------DDQDSNC---AKPQEEV 660
            +++S +   ++  S  S + +       D  K           D+  N     K +   
Sbjct: 601 GDENSQEVSNDSLVSSGSLNANGTSSCTEDFKKSGYNFQSALVQDERLNLDEFEKRRGHT 660

Query: 661 TSSPISSKEKESPTINCDPKNLSDSDDLPLKDT----PSVISSVENSDNPSHDKPSEHRE 720
            SS ISS+E ES       +++S S ++PLK+     P      + SD+P H   + +R 
Sbjct: 661 LSSSISSREDESFDCVISREDVSVSAEMPLKNVLSPKPPRSPVWQESDSPKH---AMNRY 720

Query: 721 GTEDKQKVM----------------VDDVTEPSELDVSFDDYANIHVSHDSVEINKEEEE 780
           G  +K K M                V D  E +ELDVSF++Y N  +S DS EI KEE+ 
Sbjct: 721 GQGNKSKRMEHDNEVFSMVSMGENSVKDTMEATELDVSFEEYQNSSLSQDSTEICKEED- 780

Query: 781 EEGEEGEEGEEDQSKIKSNQESETFFVGLSRNNLRDFSRSSEITDNGRPTVSVNGQPLPA 840
                         + K ++ S+TFF G  R + RDFS+S+      +P V +NGQPLP 
Sbjct: 781 --------------RFKFSKGSDTFFAGFIRKSFRDFSKSNYGGMGEKPKVFINGQPLPD 840

Query: 841 HIVKKAEKHAGPILPGDYWYDYQAGFWGVMGHPCLGIIPPFIDEFTYPMSRNCAAGNTGI 900
            +VK+AEK AGPI PGDYWYD++AGFWGVMG PCLGIIPPFI+EF YPM  NC AGNTG+
Sbjct: 841 RVVKRAEKFAGPIRPGDYWYDFRAGFWGVMGQPCLGIIPPFIEEFNYPMPTNCGAGNTGV 900

Query: 901 FVNGRELHKRDLELLSSRGLPTTTNKLYRIDISGRVIDEDSGKVLYNLGKLAPTIEKVKH 902
           +VNGREL++RDL+LL+ RGLPTT+ K Y I+ISG+V+DED+G+ L  LG+LAPT+E+ K 
Sbjct: 901 YVNGRELNQRDLDLLAERGLPTTSEKFYIIEISGKVMDEDTGEELDKLGRLAPTVERTKL 924

BLAST of Cucsa.107260.1 vs. TrEMBL
Match: B9GXG0_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0003s13750g PE=4 SV=2)

HSP 1 Score: 514.2 bits (1323), Expect = 3.2e-142
Identity = 344/953 (36.10%), Postives = 494/953 (51.84%), Query Frame = 1

Query: 6   KVRVVRCPRCQNLLPEPSGLSVYQCGGCGIVLKAKSKVLFN----EKRDFG----SSEKY 65
           KVR+VRCP+C NLLPE    SVYQCGGCG VL+AK KV  N    EK   G      EK 
Sbjct: 7   KVRLVRCPKCGNLLPELPDYSVYQCGGCGAVLRAKKKVTVNGGILEKSGMGWDEEGFEKL 66

Query: 66  ENFSEQGGSSLGGVSDSERGSPSMEPTVNERVMRSRRTV------FSNSPIRTSDREDID 125
           E+  E+ G SLG  S++ER S   E  +N R  R  RT       F  SP+  ++++DI 
Sbjct: 67  ESLPEKEGGSLGNASETERES---EGIINNR--RKARTFEETNVNFVRSPLLKAEKKDIL 126

Query: 126 DYERKIGKETKGVWPMQRLGDKEIGSVEETHSQFSEQRIENWVRRYNIEQDMNIYDSDSP 185
                I  +       Q +G       E+      +  I+NW+RR + + +MN  +S S 
Sbjct: 127 AANSNINVKE------QHVGYHSGAEKEKP----MKHPIDNWIRRDDNDMNMNRSESVSS 186

Query: 186 SK--------APYRNPTGAARTRATFEHHRVERDAFAGYSGNSMAGAHGKGAPNFRYPGD 245
           S+        A +++     R     +    +R+   G  GN          PNF YP +
Sbjct: 187 SREKGIREISAQFKSSAEFFRPTRVMDQWGSDREGLGG--GNHRVAVKQSKFPNFAYPEE 246

Query: 246 RPSSSNL-NVYYGHPEPNRNYEGP--IEGFDPNRAELLRRLDELKAEIIKSCDVGDRPR- 305
            PS+ +L +  YG  +P +NY  P  I  F+ +RAELLRRLDEL+ ++ +S  VG++ R 
Sbjct: 247 GPSNYHLGSSSYGSKQPVKNYYNPDKIAYFEQDRAELLRRLDELQEQLSRSGSVGEKQRE 306

Query: 306 ---VVADRAPVDPYYSRAGYNVPMRSSTKNPQHNHDPQYFGRGSGTFPATGHHQRNGEDF 365
              + ++ AP DPY  +   N  M   T +    + P +   G G  P    H  + ++F
Sbjct: 307 RIPMYSEIAPPDPYRHQDTSNSSMLHLTPDKHVANPPCFKYYGHGPAPYMNSHDMDTQNF 366

Query: 366 LHPPRHVVKDMPLYEDEFQEQMIRKTNHQPAHQY---PPRQHYPESVMDFKQDPLSPLHD 425
             P +     +P YED FQ+Q  R   HQ   QY   PP  H+    +DF   PL  + D
Sbjct: 367 YSPSKRSPNGIPAYEDLFQQQTPRMRPHQSPQQYLRQPPHDHFAGQHVDFSHKPL--VSD 426

Query: 426 EDVFFHH-PACSCSQCGKRNRQGPPNSPASNVSNPKEPIKSSTY----HNETPVSVGLMA 485
                HH PAC C  C  +N   P  +  +  SN K P  S+ +    H        L+ 
Sbjct: 427 SYGRSHHGPACPCFHCYNKNWHIPSQASPTTFSNKKFPKASTDFCFNQHINAVTHRPLLY 486

Query: 486 SNLPCAGRFPSQDTLPHSRQPSELDSEIDGFGLVQPRTAAVFQRNGKSRDAIAGGAPFIV 545
                      +D   H R PS+++S++DGF    P+   + + N +   +IAGGAPFI 
Sbjct: 487 HPQANPPALSPRDPQSHVRWPSDVESDMDGFPKSCPKKVVIARGNEQLCRSIAGGAPFIS 546

Query: 546 CSSCLELLKLPRKLYRLEVDWQKLQCGACSVVIIVKVENRKLVISVPAETKPTEVSPNDS 605
           C +C ELLKLPRKL   E + +KL+CG+CS  I++++++++L+ SVPAE K        S
Sbjct: 547 CCNCFELLKLPRKLKVREKNQRKLRCGSCSAFILLEIKSKRLITSVPAENKQMLAEAGIS 606

Query: 606 S---PKSVVNATSSIESSDNSSLKVIDTDHNKPSDDQD-------------SNCAKPQEE 665
           S    K ++N+   + +   +     + DH       D             S C K Q  
Sbjct: 607 SHEVSKVLLNSDGCLNAGGTTCSDDFE-DHGYDFQSADFKDVLSEERKLNTSKCEKRQSL 666

Query: 666 VTSSPISSKEKESPTINCDPKNLSDSDDLPLKD-TPSVISSVENSDNPSHDKPSEHREG- 725
            +SS ISS+E+E+       ++ S + +LP+KD  PS   S    ++ S D  S H E  
Sbjct: 667 ASSSSISSEEEENLDSLVVERDFSYAAELPVKDEVPSTFQSSPFQEH-SGDVLSSHAENK 726

Query: 726 ---------TEDKQKVMVDDVTEPS--------ELDVSFDDYANIHVSHDSVEINKEEEE 785
                    TE +  ++  ++++ S        E++VSF++Y N  VS DS E+  EE  
Sbjct: 727 CEQGNRVGWTEQENVILEKNISQQSSVNVSVATEMEVSFNEYLNTSVSQDSAEVRNEE-- 786

Query: 786 EEGEEGEEGEEDQSKIKSNQESETFFVGLSRNNLRDFSRSSEITDNGRPTVSVNGQPLPA 845
                        +++K N+ SE F +G  + + RDFSRS++   N +  V +NG+P+P 
Sbjct: 787 -------------NQLKINKGSEPFLLGFIKKSFRDFSRSNQHLPNEKLNVIINGKPIPD 846

Query: 846 HIVKKAEKHAGPILPGDYWYDYQAGFWGVMGHPCLGIIPPFIDEFTYPMSRNCAAGNTGI 887
            +VK+AEK AGPI PGDYWYD +AGFWGV G PCLGIIPPFI+EF +PM  NC+AGNT +
Sbjct: 847 CMVKRAEKLAGPIQPGDYWYDVRAGFWGVTGEPCLGIIPPFIEEFNHPMPENCSAGNTSV 906

BLAST of Cucsa.107260.1 vs. TrEMBL
Match: B9RCP8_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1691140 PE=4 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 1.1e-139
Identity = 349/972 (35.91%), Postives = 498/972 (51.23%), Query Frame = 1

Query: 6   KVRVVRCPRCQNLLPEPSGLSVYQCGGCGIVLKAKSKVLFN--------EKRDFGSSEKY 65
           KVR VRCP+C++LL E    SVY+CGGCG +L+AK KV  N         + D    EK 
Sbjct: 8   KVRFVRCPKCESLLTELPNYSVYECGGCGSLLRAKKKVTINGILVEKLERESDEDGFEKL 67

Query: 66  ENFSEQ--GGSSLGGVSDSERGSP--SMEPTVNERVMRSR--RTVFSNSPIRTSD----R 125
           E+ SE+   G  LG   + ER     ++     ERV + +    V +   I  S     R
Sbjct: 68  ESLSEKEDAGRDLGIGLEVERRKRDGALLDRGKERVFKEKTYHLVSNYKEIINSSNRIVR 127

Query: 126 EDIDDYERKIGKETKGVWPMQRLGDKEIGSVEETHSQFSEQRIENWVRRYNIEQDMNIYD 185
           E I  Y+R  GK T                  + +         NWV     E ++N ++
Sbjct: 128 EQIVGYQRGEGKGT---------------DYNDGYKAPLNHPARNWVGNDGEEMNINGFE 187

Query: 186 SDSPSK--------APYRNPTGAARTRATFEHHRVERDAFAGYSGNSMAGAHGKGAPNFR 245
           S + S+        A ++  TG+ R+RA      + RD   G  GN           NF 
Sbjct: 188 SVNLSREKDMENISAQFKGSTGSLRSRAMVGKWGLNRDGSGGRHGNLRIADERSNVSNFA 247

Query: 246 -YPGDRPSSSNL--NVYYGHPEPNRNYEGPIEGFDPNRAELLRRLDELKAEIIKSCDVGD 305
            YP + PS+ N   +  +GH +  +N++   E  +P+RAEL+R+LDELK +I +S    +
Sbjct: 248 SYPDEGPSNYNYVKSSSHGHGQLLKNFDNRFEHLEPDRAELIRKLDELKEQICRSSSAAE 307

Query: 306 RPRVVADRAPVDPY-YSRAGYNVPMRSSTKNPQHNHDPQYFGRGSGTFPATGHHQRNGED 365
           +      + P   Y + RA YN P++    + +H   P YF +        GH  RN ED
Sbjct: 308 K------QTPPGFYNHDRAPYNFPVKPLAPD-KHVPRPLYFRQND-----YGHVVRNSED 367

Query: 366 -----FLHPPRHVVKDMPLYEDEFQEQMIRKTNHQPAHQY---PPRQHYPESVMDFKQDP 425
                F  PP+H     P+Y++  Q QM R  +H    +Y   P R +Y  +   F ++P
Sbjct: 368 MNMQNFYFPPKHNANKFPVYKNSVQLQMPRSDSHPAPDRYSQQPLRDYYLGTHEGFDREP 427

Query: 426 LSPLHDEDVFFHHPACSCSQCGKRNRQGPPNSPASNVSNP---KEPIKSSTYHNETPVSV 485
           L+  +     +H PAC+C  C  +    P   PAS        +EP  S+  H    +  
Sbjct: 428 LAS-YPRGTMYHKPACACFHCYNKKWHVPSQVPASVFGRKYFMEEPTVSNFNHQVDHIK- 487

Query: 486 GLMASNLPCAGR--FPSQDTLPHSRQPSELDSEIDGFGLVQPRTAAVFQRNGKSRDAIAG 545
               +  P        S+D       PS++DS +D F         V   +G+    I G
Sbjct: 488 SRSGNPTPQVNHRALHSRDAQSDIGWPSDIDSNMDVFRHSHLGRVVVAHGDGRICHPITG 547

Query: 546 GAPFIVCSSCLELLKLPRKLYRLEVDWQKLQCGACSVVIIVKVENRKLVISVPAETKPTE 605
           GAPFI CSSC E LKLPRK    E + QKLQCGACS V+ +++ N+KLV+S+P + K   
Sbjct: 548 GAPFIACSSCFESLKLPRKCKLREKNQQKLQCGACSTVLFIEIRNKKLVMSIPVKNKQIL 607

Query: 606 VSPNDSS-------PKSVVNATSSIESSDNSSL----KVIDTDHNKPSDDQDSNC--AKP 665
               D S       P+   NA  +  S D  ++    +  D   N   +D+  N   ++ 
Sbjct: 608 AEAADGSRGEGLWSPEGDFNAEGTNCSVDLDNVGYDFQSADFKGNVLPEDRRLNLNESRA 667

Query: 666 QEEVTS-SPISSKEKESPTINCDPKNLSDSDDLPLKDT-------PSVISSVENSDNPSH 725
           +  +TS S +SS E +        ++LSD  +LP KD        P  + S++  D  S+
Sbjct: 668 RHSLTSLSSVSSGEDKITDSMIVQRDLSDFPELPTKDDTSSTYLRPLFMESLD--DISSN 727

Query: 726 DKPSEHREGTEDKQKVMVD----------DVTEPSELDVSFDDYANIHVSHDSVEINKEE 785
           +K + + EG E  ++V++D          +++  +E++VSF ++ N   S DS+E++KE+
Sbjct: 728 NKINRNGEGDESNKEVILDKDACRENNLNNISSETEVEVSFSEFLNTTTSQDSLEVSKED 787

Query: 786 EEEEGEEGEEGEEDQSKIKSNQESETFFVGLSRNNLRDFSRSSEITDNGRPTVSVNGQPL 845
            +                + N+ SE+F VGL + + RDFSRS++  +  +P V VNGQP+
Sbjct: 788 LQP---------------RINKGSESFLVGLLKKSFRDFSRSNQHNEYEKPNVFVNGQPI 847

Query: 846 PAHIVKKAEKHAGPILPGDYWYDYQAGFWGVMGHPCLGIIPPFIDEFTYPMSRNCAAGNT 904
           P  +VKKAEK AGPI PGDYWYD QAGFWG+MG PCLGIIPP I EF YPM  NC+ GNT
Sbjct: 848 PDSMVKKAEKLAGPIHPGDYWYDSQAGFWGLMGQPCLGIIPPCIKEFNYPMPENCSLGNT 907

BLAST of Cucsa.107260.1 vs. TAIR10
Match: AT3G61670.1 (AT3G61670.1 Protein of unknown function (DUF3133))

HSP 1 Score: 314.7 bits (805), Expect = 1.9e-85
Identity = 222/708 (31.36%), Postives = 357/708 (50.42%), Query Frame = 1

Query: 219 RYPGDRPSSSNLNVYYGHPEPNRNYEGPI-----EGFDPNRAELLRRLDELKAEIIKSCD 278
           R+P D  +SS+ N +   P+    ++  +     E  + +RA LLR+L+++K ++++SC+
Sbjct: 158 RHPCDEGTSSSANYF---PDSLLEFQKHLKDQSNEAIEQDRAGLLRQLEKIKEQLVQSCN 217

Query: 279 VG-DRPRVVADRAPVDPYYSRAGYNVPMRSSTKNPQHNHDPQYFGRGSGTFPATGHHQRN 338
           V  D+ +   ++AP     S      PMR                     F +TG+H   
Sbjct: 218 VATDKSK---EQAPSSSSASGLNKAPPMR---------------------FHSTGNHAVG 277

Query: 339 GEDFLHPPRHVVKDMPLYEDEFQEQMIRKTNHQPAHQYPPRQHYPESVMDFK-------- 398
           G  + H P+    +  + E      ++  +   P H++P     P      +        
Sbjct: 278 GPSYYHQPQFPYNNNNINEAPMHHSLMHPSYGDP-HRFPIHGRGPHPYFSGQYVGNNNNG 337

Query: 399 QDPLSPLHDEDVFFHHPACSCSQCGKRNRQGPPNSPASNVSNPKEPIKSSTYHNETPVSV 458
            D       ++  FHH +CSC  C   N+    ++P      P  P  +  Y +E+ +  
Sbjct: 338 HDLFDAYPQQNGHFHHSSCSCYHCYD-NKYWRGSAPVV----PDAPYNAGFYPHESVM-- 397

Query: 459 GLMASNLPCAGRFPSQDTLPHSRQPSEL-DSEIDGFGLVQPRTAAVFQRNGKSRDA--IA 518
           G    + P    + S+   PH R PS   D+++D    ++P    +   +G SR    +A
Sbjct: 398 GFAPPHNPRT--YGSRGLQPHGRWPSNFSDAQMDALSRIRPPKVVL---SGGSRHIRPLA 457

Query: 519 GGAPFIVCSSCLELLKLPRKLYRLEVDWQKLQCGACSVVIIVKVENRKLVISVPAETKPT 578
           GGAPFI C +C ELL+LP+K        QK++CGACS +I + V N K V+S        
Sbjct: 458 GGAPFITCQNCFELLQLPKKPEAGTKKQQKVRCGACSCLIDLSVVNNKFVLST------- 517

Query: 579 EVSPNDSSPKSVVNATSSIESSDNSSLK--VIDTDHNKPSDDQD--SNCAKPQEEVTSSP 638
               N +S +      ++  +SD+  L   V  +  ++P D     S+ ++  + V S  
Sbjct: 518 ----NTASTRQGEARVAADYTSDDYDLLGYVFHSLDDEPRDLPGLISDKSQDMQHVHSHS 577

Query: 639 ISSKEKESPTINCDPKNLSDSDDLPLKDTPSVISSVENSDNPSHDKPSEHREGTEDKQKV 698
            S  E E  + +   K L+++ +    D  S+  + + S   S    SEH + T  K   
Sbjct: 578 ASLSEGELSSDSLTAKPLAEAHE-NFVDYSSI--NHDRSGAGSRSSRSEHDKVTLSKATA 637

Query: 699 M----VDDVTEPSELDVSFDDYANIHVSHDSVEINKEEEEEEGEEGEEGEEDQSKIKSNQ 758
           M    + +V+  SE++V+F+DY     SH +  ++K++++   + G              
Sbjct: 638 MRQNSMKEVSLASEMEVNFNDY-----SHRNSGVSKDQQQRAKKSG-------------- 697

Query: 759 ESETFFVGLSRNNLRDFSRSSEITDNGRPTVSVNGQPLPAHIVKKAEKHAGPILPGDYWY 818
                F  + + + +D ++S +  +  +  VS+NG PL   +++KAEK AG I PG+YWY
Sbjct: 698 -----FASIVKKSFKDLTKSIQNDEGNKSNVSINGHPLTERLLRKAEKQAGVIQPGNYWY 757

Query: 819 DYQAGFWGVMGHPCLGIIPPFIDEFTYPMSRNCAAGNTGIFVNGRELHKRDLELLSSRGL 878
           DY+AGFWGVMG P LGI+PPFI+E  YPM  NC+ G TG+FVNGRELH++DL+LL+ RGL
Sbjct: 758 DYRAGFWGVMGGPGLGILPPFIEELNYPMPENCSGGTTGVFVNGRELHRKDLDLLAGRGL 787

Query: 879 PTTTNKLYRIDISGRVIDEDSGKVLYNLGKLAPTIEKVKHGFGMKVPR 902
           P   ++ Y +DI+GRVIDED+G+ L  LGKLAPTIEK+K GFGM++P+
Sbjct: 818 PPDRDRSYIVDITGRVIDEDTGEELDCLGKLAPTIEKLKRGFGMRLPK 787

HSP 2 Score: 58.5 bits (140), Expect = 2.4e-08
Identity = 25/42 (59.52%), Postives = 32/42 (76.19%), Query Frame = 1

Query: 1  MATAQKVRVVRCPRCQNLLPEPSGLSVYQCGGCGIVLKAKSK 43
          MA + KVR+VRCP+C+NLL EP     +QCGGC  VL+AK+K
Sbjct: 1  MADSTKVRLVRCPKCENLLSEPEDSPFFQCGGCFTVLRAKTK 42

BLAST of Cucsa.107260.1 vs. TAIR10
Match: AT1G01440.1 (AT1G01440.1 Protein of unknown function (DUF3133))

HSP 1 Score: 301.6 bits (771), Expect = 1.6e-81
Identity = 236/704 (33.52%), Postives = 338/704 (48.01%), Query Frame = 1

Query: 223 DRPSSSNLNVYYGHPEPNRNYEGPIEGFDPNRAELLRRLDELKAEIIKSCDVGDRPRVVA 282
           D  SSS+ N         RN   PI+  D  +AELLR+LD +K  +++    G     V 
Sbjct: 62  DSDSSSSKNT------TTRN--NPIQYTDKQQAELLRKLDSIKDHLLRG---GGNNATVV 121

Query: 283 DRAPVDPYYSRAGYNVPMRSSTKNPQHNHDPQYFGRGSGTFPATGHHQRNGEDFLHPPRH 342
           D+ P+       G++           H   P Y+      FP            ++P   
Sbjct: 122 DQPPM-------GFHA---------HHGPPPSYYNPYPEPFPYG----------MYPTAS 181

Query: 343 VVKDMPLYEDEF-------QEQMIRKTNHQPAHQYPPRQHYPES-VMDFKQDPLSPLHDE 402
               +P Y D +        +   +  +H P +Q PPR  YP+   +D   D L     +
Sbjct: 182 NQPHVPAYRDPYGFPVHRIPQNFYQGPSHYP-NQMPPRPPYPQGQYVDIGSDILESQLQD 241

Query: 403 DVFFHHPACSCSQCGKRNRQGPPNSPASNVSNPKEPIKSSTYHNETPVSVGLMASNLPCA 462
             FF                  P +P+     P  P   + +H E               
Sbjct: 242 PRFF------------------PGTPSRYGDVPFSP---ALHHGEK-------------V 301

Query: 463 GRFPSQDTLPHSRQPSELDSEIDGF---GLVQPRTAAVFQRNGKSRDAIAGGAPFIVCSS 522
           G F     + H+R PSE+DSE+ G    G VQ    AV   + +    +AGGAPFI C S
Sbjct: 302 GPFSPHGGV-HTRWPSEIDSEMGGAFARGYVQQ---AVSDTDSRRCHPLAGGAPFIACHS 361

Query: 523 CLELLKLPRKLYRLEVDWQKLQCGACSVVIIVKVENRKLVISVPA---ETKPTEVSPND- 582
           C ELL +P+K    +   QK+QCGACS VI  +V ++KLV S  A    T    V   D 
Sbjct: 362 CFELLYVPKKKLLGQERQQKMQCGACSEVITFRVVDKKLVFSSSALGETTNRVSVEVEDR 421

Query: 583 SSPKSVVN--ATSSIESSDNSSLKVID----TDHNKPSDDQDSNCAKPQEEVTSSPISSK 642
           SSP  VV+    +  E   +   K++     +DH+   +D+ S  ++P+++V  S  S  
Sbjct: 422 SSPIPVVDDYPLNDEEPRIHQETKIVHAVSPSDHSN-DEDRSSISSEPRKQVVKSVRSRA 481

Query: 643 E--KESPTINCDPKNLSDSDDLPLKDTPSVISSVENSDNPSHDKPSEHREGTEDKQKVMV 702
           +  K  P    +  NL +  +    +  ++   +             +++ +  KQ  + 
Sbjct: 482 QGAKVPPPPPPEKSNLLELFEYSNVNRAAITYGMAQL--------GYYKQESYTKQDSLK 541

Query: 703 DDVTEPSELDVSFDDYANIHVSHDSVEINKEEEEEEGEEGEEGEEDQSKIKSNQESETFF 762
            + +  +E DVS+++Y               EE E+    +  +E +      Q SE  F
Sbjct: 542 SE-SVATETDVSYNEY-----------YTNTEESEDSRISKASKEGRRPRNRKQSSEHSF 601

Query: 763 VGLSRNNLRDFSRSSEITDNGRPTVSVNGQPLPAHIVKKAEKHAGPILPGDYWYDYQAGF 822
             ++ N       SS   +N +  V VNG  +P  +V  AEK AGP+  G YWYDY+AGF
Sbjct: 602 AEVTNNI------SSNDQNNEQLEVWVNGYLIPEDLVISAEKQAGPVQAGKYWYDYRAGF 661

Query: 823 WGVMGHPCLGIIPPFIDEFTYPMSRNCAAGNTGIFVNGRELHKRDLELLSSRGLPTTTNK 882
           WGVMG+PCLGIIPPFI+EF+ PM  NC AGNT +FVNGRELH+RDLELLSSRGLP   N+
Sbjct: 662 WGVMGNPCLGIIPPFIEEFSRPMPDNCGAGNTSVFVNGRELHERDLELLSSRGLPRGKNR 662

Query: 883 LYRIDISGRVIDEDSGKVLYNLGKLAPTIEKVKHGFGMKVPRTL 904
            Y IDI+GRV+D DSG+ L +LG+LAPT++KVKHGFGM+VPR+L
Sbjct: 722 SYIIDIAGRVLDGDSGEELKSLGRLAPTVDKVKHGFGMRVPRSL 662

BLAST of Cucsa.107260.1 vs. TAIR10
Match: AT4G01090.1 (AT4G01090.1 Protein of unknown function (DUF3133))

HSP 1 Score: 298.5 bits (763), Expect = 1.4e-80
Identity = 251/735 (34.15%), Postives = 351/735 (47.76%), Query Frame = 1

Query: 205 NSMAGAHGKGAPNFRYPGDRPSSSNLNVYYGHPEPNRNYEG---PIEGFDPNRAELLRRL 264
           NS AG+   G        D P+       YG    ++N      PIE  +  RAELLRRL
Sbjct: 34  NSRAGSQRSGKS---LRNDEPN-------YGSDSSSKNTSSSNNPIEYTEHERAELLRRL 93

Query: 265 DELKAEIIKSCDVGDRPRVVADRAPVDPYYSRAGYNVPMRSSTKNPQHNHDPQYFGRGSG 324
           D +K  +++    G+    V D+ P +P  ++            N  HN  P Y+ +   
Sbjct: 94  DSIKDHLLRGGGGGNN---VVDK-PKEPDQNQPFLRPVHLHGHAN--HNPGPSYYHQYPE 153

Query: 325 TFPATGH-HQRNGEDFLHPPRHVVKDMPLYEDEFQEQMIRKTNHQPAHQYPPRQHY---P 384
                G  H    + +  P  +  +  P+    +         +Q A QYP  Q+    P
Sbjct: 154 PIQYPGPVHGMYPQAYQDPYGYQRRPPPVPNPNWYPP--GHYPNQMARQYPGGQYVELGP 213

Query: 385 ESVMDFKQDPLSPLHDEDVFFHHPACSCSQCGKRNRQGPPNSPASNVSNPKEPIKSSTYH 444
           + V      P +P    D+  + P  S  +  K   Q     P S VS+     K +T +
Sbjct: 214 DIVEPHSYYPATPGRYGDLPPYSPVSSHHREEKLATQYSDMPPYSPVSSHHRGEKLTTPY 273

Query: 445 NETPVSVGLMASNLPCAGRFPSQDTLP-----HSRQPSELDSEIDGFGLVQPRTAAVFQR 504
           +   V+ G           FPS    P     ++R PSE DSE+ G         AV   
Sbjct: 274 SPR-VNNG---------SSFPSSMGTPGPRGGYARWPSEHDSEMGGAFARGYVKKAVSDT 333

Query: 505 NGKSRDAIAGGAPFIVCSSCLELLKLPRKLYRLEVDWQKLQCGACSVVIIVKVENRKLVI 564
             +    +AGGAPFI C SC ELL LP+K    +    KLQCGACS VI   + +RKLV 
Sbjct: 334 GVRRCHPLAGGAPFIACHSCFELLYLPKKKLLSQERLHKLQCGACSEVISFTIVDRKLVF 393

Query: 565 SVP-AETKPTEVSPNDSSPKSVVNATSSIESSD-----------NSSLKVIDTDHNKPSD 624
           S    ETKP  +   D +  + V     + S D           +    V +  +++ + 
Sbjct: 394 SSGNEETKPVSLEVEDRNTTNTVVIEEEVSSVDFNSSGRDIPRKDEEEPVQELRNHQDTI 453

Query: 625 DQDSNCAKPQ----EEVTSSPISSKEKESPTINCDPKNLSDSDDLPLKDTPSVISSVENS 684
              S  ++ Q    EE +S+    ++KE  ++    K  S + +    D  S++   E S
Sbjct: 454 TTQSVRSESQHSDDEERSSNSSEQQQKEVKSVRRRGKG-SKAPEPAAPDNASLLELFEYS 513

Query: 685 D-NPSHDKPSEHREGTE--DKQK-VMVDDVTEP----SELDVSFDDYANIHVSHDSVEIN 744
           + N +       + G E  DKQK  M  D  +P    +E +VS++ Y+N  +S DS   N
Sbjct: 514 NVNRAALAYGMAQLGYEKPDKQKSFMTQDSLKPESVATETEVSYNGYSNTEISEDSRYSN 573

Query: 745 KEEEEEEGEEGEEGEEDQSKIKSNQESETFFVGLSRNNLRDFSRSSEITDNGRPTVSVNG 804
           ++  E        G  + +   S  ++E            D  +S E+         VNG
Sbjct: 574 RKGSEY-------GSTEITTRSSTDQNE------------DQMKSLEVW--------VNG 633

Query: 805 QPLPAHIVKKAEKHAGPILPGDYWYDYQAGFWGVMGHPCLGIIPPFIDEFTYPMSRNCAA 864
             +P  +V  AEK AGPI  G YWYDY+AGFWGVMG PCLGIIPPFI+EF++PM  NCAA
Sbjct: 634 HLIPEDLVSSAEKLAGPIQAGKYWYDYRAGFWGVMGKPCLGIIPPFIEEFSHPMLDNCAA 693

Query: 865 GNTGIFVNGRELHKRDLELLSSRGLPTTTNKLYRIDISGRVIDEDSGKVLYNLGKLAPTI 904
           GNT +FVNGRELHKRD ELL  RGLP   N+ Y +DISGR++D+DSG+ L++LGKLAPTI
Sbjct: 694 GNTDVFVNGRELHKRDFELLVGRGLPRDKNRSYIVDISGRILDQDSGEELHSLGKLAPTI 712

BLAST of Cucsa.107260.1 vs. TAIR10
Match: AT2G46380.1 (AT2G46380.1 Protein of unknown function (DUF3133))

HSP 1 Score: 291.6 bits (745), Expect = 1.7e-78
Identity = 221/680 (32.50%), Postives = 323/680 (47.50%), Query Frame = 1

Query: 248 EGFDPNRAELLRRLDELKAEIIKSCDVGDRPRVVADRAPVDPYYSRAGYNVPMRSSTKNP 307
           E  + +RA L+R+LD+LK ++++             +    P  S      P+R  +   
Sbjct: 147 EAIEQDRAGLVRKLDKLKEQLVQP------------KEQQIPSSSSGFQKAPLRVFSSG- 206

Query: 308 QHNHDPQYFGRGSGTFPATGHHQRNGEDFLHPPRHVVKDMPLYEDEFQEQMIRKTNHQPA 367
           +    P Y+   S   P   ++  N +  LH P H   ++P Y D     M  +T  QP+
Sbjct: 207 RRIAGPSYYQYYSEPEPPCLYNN-NSDVSLHGPMHNPANVPAYGDPHGFPMHERTL-QPS 266

Query: 368 HQYPPRQHYPESVMDFKQDPLSPLHDEDVFFHHPACSCSQCGKRNRQG-----PPNSPAS 427
           H +  RQ+   +  D     +   H  +  FH   CSC+ C     +      PP+    
Sbjct: 267 HLHNSRQYIGNNGHD-----VFDTHPHNGMFHQSTCSCTHCYDPYHRASGSVFPPSGLPD 326

Query: 428 NVSNP-------KEPIKSSTYHNETPVSVGLMASNLPCAGR----FPSQDTLPHSR-QPS 487
            + NP            +S +   T +  G  +      GR    F        SR  PS
Sbjct: 327 ALRNPGFYPHERSFGFGTSLHSPRTFIPPGSQSPGPQLRGRCTSGFKDMRMNAVSRVHPS 386

Query: 488 ELDSEIDGFGLVQPRTAAVFQRNGKSRDAIAGGAPFIVCSSCLELLKLPRKLYRLEVDWQ 547
           +  S   G  L+QP               +AGGAPFI C +C +LLKLP K+       Q
Sbjct: 387 KPVSSSGGSRLIQP---------------VAGGAPFINCINCFKLLKLPEKIDSATRKKQ 446

Query: 548 KLQCGACSVVIIVKVENRKLVISV-PAETKPTEVSPNDSSPKSVVNATSSIESSDNSSLK 607
           +++CGACS VI     ++KL++S  PA  +  E     S  +    A  S +  DN++ +
Sbjct: 447 RMRCGACSCVIDYSFVDKKLILSTDPASARKPETH---SRLRWETTANFSSDDYDNAAYE 506

Query: 608 VIDTDHNKPSDDQD----SNCAKPQE--EVTSSPISSKEKESPTINCDPKNLSDSDDLPL 667
               D             SN A+  +  + TS  +S  E  S +      NL+    L  
Sbjct: 507 FHAMDRGSADVSTALVLISNTAQEMQITDATSPSVSEDELSSDSSTVRKVNLASP--LHK 566

Query: 668 KDTPSVISSVENSDNPSHDKPSEHREGTEDKQKVMVDDVTEPS-ELDVSFDDYA-NIHVS 727
           +   S I+  + S   S    SE    T  K  +  + + E S  +++  +DY+ N  VS
Sbjct: 567 QFEYSSINVRDRSGPRSQSSRSEQDRVTLSKTAMRQNSMKEASVAIEMDVNDYSHNNEVS 626

Query: 728 HDSVEINKEEEEEEGEEGEEGEEDQSKIKSNQESETFFVGLSRNNLRDFSRSSEITDNGR 787
            DS                +  +DQ + K        F  + +N+ +D  +S  I + GR
Sbjct: 627 QDSAN--------------DCTDDQGRTKKGG-----FASIMKNSFKDLKKS--IQNEGR 686

Query: 788 PTVSVNGQPLPAHIVKKAEKHAGPILPGDYWYDYQAGFWGVMGHPCLGIIPPFIDEFTYP 847
             VS+NG P+   +VK AEK AGPI PG+YWYDY+AGFWGV+G  CLGI+PPFI+E  YP
Sbjct: 687 SDVSINGHPVAERLVKMAEKQAGPIRPGNYWYDYRAGFWGVLGSHCLGILPPFIEELNYP 746

Query: 848 MSRNCAAGNTGIFVNGRELHKRDLELLSSRGLPTTTNKLYRIDISGRVIDEDSGKVLYNL 902
           M  NCA G T +FVNGRELH++DL LL++RGLP   ++ Y + ISGRVIDED+G+ L +L
Sbjct: 747 MPENCAGGTTRVFVNGRELHQKDLRLLTARGLPRDRDRSYTVYISGRVIDEDTGEELDSL 765

HSP 2 Score: 56.6 bits (135), Expect = 9.1e-08
Identity = 29/76 (38.16%), Postives = 44/76 (57.89%), Query Frame = 1

Query: 6  KVRVVRCPRCQNLLPEPSGLSVYQCGGCGIVLKAKSKVLFNEKRDFGSSEKYENFSEQGG 65
          + R+VRCP+C+NLL EP     +QCGGCG VL AK+K   + + D  S +  EN S++  
Sbjct: 8  EARLVRCPKCKNLLQEPKDCPFFQCGGCGTVLCAKNK---DREADLLSVKSVENRSKEIE 67

Query: 66 SSLGGVSDSERGSPSM 82
           + G   +    + S+
Sbjct: 68 VTSGSSEEESDSNASL 80

BLAST of Cucsa.107260.1 vs. TAIR10
Match: AT2G23460.1 (AT2G23460.1 extra-large G-protein 1)

HSP 1 Score: 67.0 bits (162), Expect = 6.8e-11
Identity = 39/112 (34.82%), Postives = 58/112 (51.79%), Query Frame = 1

Query: 764 VSVNGQPLPAHIVKKAEKHAGP---ILPGDYWYDYQAGFWGVMGHPCLGIIPPFIDEFTY 823
           V VNGQPL    +   +  + P   + PGDYWYD  +G WG  G     II P ++    
Sbjct: 317 VYVNGQPLYPEELVTLQTCSNPPKKLKPGDYWYDKVSGLWGKEGEKPYQIISPHLN-VGG 376

Query: 824 PMSRNCAAGNTGIFVNGRELHKRDLELLSSRGLPTTTNKLYRIDISGRVIDE 873
           P+S   + GNT +F+NGRE+ K +L +L   G+    N  + ++  G   +E
Sbjct: 377 PISPEASNGNTQVFINGREITKVELRMLQLAGVQCAGNPHFWVNEDGSYQEE 427

BLAST of Cucsa.107260.1 vs. NCBI nr
Match: gi|778691015|ref|XP_011653210.1| (PREDICTED: uncharacterized protein At5g05190 isoform X1 [Cucumis sativus])

HSP 1 Score: 1857.8 bits (4811), Expect = 0.0e+00
Identity = 904/910 (99.34%), Postives = 904/910 (99.34%), Query Frame = 1

Query: 1   MATAQKVRVVRCPRCQNLLPEPSGLSVYQCGGCGIVLKAKSKVLFNEKRDFGSSEKYENF 60
           MATAQKVRVVRCPRCQNLLPEPSGLSVYQCGGCGIVLKAKSKVLFNEKRDFGSSEKYENF
Sbjct: 1   MATAQKVRVVRCPRCQNLLPEPSGLSVYQCGGCGIVLKAKSKVLFNEKRDFGSSEKYENF 60

Query: 61  SEQGGSSLGGVSDSERGSPSMEPTVNERVMRSRRTVFSNSPIRTSDREDIDDYERKIGKE 120
           SEQGGSSLGGVSDSERGSPSMEPTVNERVMRSRRTVFSNSPIRTSDREDIDDYERKIGKE
Sbjct: 61  SEQGGSSLGGVSDSERGSPSMEPTVNERVMRSRRTVFSNSPIRTSDREDIDDYERKIGKE 120

Query: 121 TKGVWPMQRLGDKEIGSVEETHSQFSEQRIENWVRRYNIEQDMNIYDSDSPSKAPYRNPT 180
           TKGVWPMQRLGDKEIGSVEETHSQFSEQRIENWVRRYNIEQDMNIYDSDSPSKAPYRNPT
Sbjct: 121 TKGVWPMQRLGDKEIGSVEETHSQFSEQRIENWVRRYNIEQDMNIYDSDSPSKAPYRNPT 180

Query: 181 GAARTRATFEHHRVERDAFAGYSGNSMAGAHGKGAPNFRYPGDRPSSSNLNVYYGHPEPN 240
           GAARTRATFEHHRVERDAFAGYSGNSMAGAHGKGAPNFRYPGDRPSSSNLNVYYGHPEPN
Sbjct: 181 GAARTRATFEHHRVERDAFAGYSGNSMAGAHGKGAPNFRYPGDRPSSSNLNVYYGHPEPN 240

Query: 241 RNYEGPIEGFDPNRAELLRRLDELKAEIIKSCDVGDRPRVVADRAPVDPYYSRAGYNVPM 300
           RNYEGPIEGFDPNRAELLRRLDELKAEIIKSCDVGDRPRVVADRAPVDPYYSRAGYNVPM
Sbjct: 241 RNYEGPIEGFDPNRAELLRRLDELKAEIIKSCDVGDRPRVVADRAPVDPYYSRAGYNVPM 300

Query: 301 RSSTKNPQHNHDPQYFGRGSGTFPATGHHQRNGEDFLHPPRHVVKDMPLYEDEFQEQMIR 360
           RSSTKNPQHNHDPQYFGRGSGTFPATGHHQRNGEDFLHPPRHVVKDMPLYEDEFQEQMIR
Sbjct: 301 RSSTKNPQHNHDPQYFGRGSGTFPATGHHQRNGEDFLHPPRHVVKDMPLYEDEFQEQMIR 360

Query: 361 KTNHQPAHQYPPRQHYPESVMDFKQDPLSPLHDEDVFFHHPACSCSQCGKRNRQGPPNSP 420
           KTNHQPAHQYPPRQHYPESVMDFKQDPLSPLHDEDVFFHHPACSCSQCGKRNRQGPPNSP
Sbjct: 361 KTNHQPAHQYPPRQHYPESVMDFKQDPLSPLHDEDVFFHHPACSCSQCGKRNRQGPPNSP 420

Query: 421 ASNVSNPKEPIKSSTYHNETPVSVGLMASNLPCAGRFPSQDTLPHSRQPSELDSEIDGFG 480
           ASNVSNPKEPIKSSTYHNETPVSVGLMASNLPCAGRFPSQDTLPHSRQPSELDSEIDGFG
Sbjct: 421 ASNVSNPKEPIKSSTYHNETPVSVGLMASNLPCAGRFPSQDTLPHSRQPSELDSEIDGFG 480

Query: 481 LVQPRTAAVFQRNGKSRDAIAGGAPFIVCSSCLELLKLPRKLYRLEVDWQKLQCGACSVV 540
           LVQPRTAAVFQRNGKSRDAIAGGAPFIVCSSCLELLKLPRKLYRLEVDWQKLQCGACSVV
Sbjct: 481 LVQPRTAAVFQRNGKSRDAIAGGAPFIVCSSCLELLKLPRKLYRLEVDWQKLQCGACSVV 540

Query: 541 IIVKVENRKLVISVPAETKPTEVSPNDSSPKSVVNATSSIESSDNSSLKVIDTDHNKPSD 600
           IIVKVENRKLVISVPAETKPTEVSPNDSSPKSVVNATSSIESSDNSSLKVIDTDHNKPSD
Sbjct: 541 IIVKVENRKLVISVPAETKPTEVSPNDSSPKSVVNATSSIESSDNSSLKVIDTDHNKPSD 600

Query: 601 DQDSNCAKPQEEVTSSPISSKEKESPTINCDPKNLSDSDDLPLKDTPSVISSVENSDNPS 660
           DQDSNCAKPQEEVTSSPISSKEKESPTINCDPKNLSDSDDLPLKDTPSVISSVENSDNPS
Sbjct: 601 DQDSNCAKPQEEVTSSPISSKEKESPTINCDPKNLSDSDDLPLKDTPSVISSVENSDNPS 660

Query: 661 HDKPSEHREGTEDKQKVMVDDVTEPSELDVSFDDYANIHVSHDSVEINKEEEE------E 720
           HDKPSEHREGTEDKQKVMVDDVTEPSELDVSFDDYANIHVSHDSVEINKEEEE      E
Sbjct: 661 HDKPSEHREGTEDKQKVMVDDVTEPSELDVSFDDYANIHVSHDSVEINKEEEEEEGEEGE 720

Query: 721 EGEEGEEGEEDQSKIKSNQESETFFVGLSRNNLRDFSRSSEITDNGRPTVSVNGQPLPAH 780
           EGEEGEEGEEDQSKIKSNQESETFFVGLSRNNLRDFSRSSEITDNGRPTVSVNGQPLPAH
Sbjct: 721 EGEEGEEGEEDQSKIKSNQESETFFVGLSRNNLRDFSRSSEITDNGRPTVSVNGQPLPAH 780

Query: 781 IVKKAEKHAGPILPGDYWYDYQAGFWGVMGHPCLGIIPPFIDEFTYPMSRNCAAGNTGIF 840
           IVKKAEKHAGPILPGDYWYDYQAGFWGVMGHPCLGIIPPFIDEFTYPMSRNCAAGNTGIF
Sbjct: 781 IVKKAEKHAGPILPGDYWYDYQAGFWGVMGHPCLGIIPPFIDEFTYPMSRNCAAGNTGIF 840

Query: 841 VNGRELHKRDLELLSSRGLPTTTNKLYRIDISGRVIDEDSGKVLYNLGKLAPTIEKVKHG 900
           VNGRELHKRDLELLSSRGLPTTTNKLYRIDISGRVIDEDSGKVLYNLGKLAPTIEKVKHG
Sbjct: 841 VNGRELHKRDLELLSSRGLPTTTNKLYRIDISGRVIDEDSGKVLYNLGKLAPTIEKVKHG 900

Query: 901 FGMKVPRTLK 905
           FGMKVPRTLK
Sbjct: 901 FGMKVPRTLK 910

BLAST of Cucsa.107260.1 vs. NCBI nr
Match: gi|659102120|ref|XP_008451962.1| (PREDICTED: uncharacterized protein At5g05190-like isoform X1 [Cucumis melo])

HSP 1 Score: 1702.6 bits (4408), Expect = 0.0e+00
Identity = 829/903 (91.81%), Postives = 859/903 (95.13%), Query Frame = 1

Query: 6   KVRVVRCPRCQNLLPEPSGLSVYQCGGCGIVLKAKSKVLFNEKRDFGSSEKYENFSEQGG 65
           KVRVVRCPRCQNLLPEPSGL VYQCGGCGIVLKAKS+VLFNEKRDFGSSEKYEN SEQGG
Sbjct: 12  KVRVVRCPRCQNLLPEPSGLPVYQCGGCGIVLKAKSEVLFNEKRDFGSSEKYENLSEQGG 71

Query: 66  SSLGGVSDSERGSPSMEPTVNERVMRSRRTVFSNSPIRTSDREDIDDYERKIGKETKGVW 125
           SSLGGVSDSE  SPSMEPTVNERVMRSRRTVFSNSPIRTSDREDIDDYERKIGKETKGVW
Sbjct: 72  SSLGGVSDSEWDSPSMEPTVNERVMRSRRTVFSNSPIRTSDREDIDDYERKIGKETKGVW 131

Query: 126 PMQRLGDKEIGSVEETHSQFSEQRIENWVRRYNIEQDMNIYDSDSPSKAPYRNPTGAART 185
            MQRLGDKE+GSVEE HSQFSEQRIENWVRRYNIEQDMNIYD DSPS APYRNP G ART
Sbjct: 132 TMQRLGDKEVGSVEEPHSQFSEQRIENWVRRYNIEQDMNIYDYDSPSTAPYRNPIGVART 191

Query: 186 RATFEHHRVERDAFAGYSGNSMAGAHGKGAPNFRYPGDRPSSSNLNVYYGHPEPNRNYEG 245
           RATFEHHRVERDAF GYSGNSMAGAHGKGAPNFRY  DRPSSSNL+++YGHPEPNRNYEG
Sbjct: 192 RATFEHHRVERDAFTGYSGNSMAGAHGKGAPNFRYSSDRPSSSNLDLFYGHPEPNRNYEG 251

Query: 246 PIEGFDPNRAELLRRLDELKAEIIKSCDVGDRPRVVADRAPVDPYYSRAGYNVPMRSSTK 305
           PIEG DPNRAELLR+LDELKA+IIKSCDV DRPRVVADRAPVDPYY RAGYNV MRSSTK
Sbjct: 252 PIEGLDPNRAELLRKLDELKAQIIKSCDVVDRPRVVADRAPVDPYYGRAGYNVSMRSSTK 311

Query: 306 NPQHNHDPQYFGRGSGTFPATGHHQRNGEDFLHPPRHVVKDMPLYEDEFQEQMIRKTNHQ 365
           +PQH + PQYFGRGSGTFPATG HQRNGEDFLHPPRHVVKDMPLYED+FQEQM+RKTNHQ
Sbjct: 312 SPQHINSPQYFGRGSGTFPATGPHQRNGEDFLHPPRHVVKDMPLYEDQFQEQMVRKTNHQ 371

Query: 366 PAHQYPPRQHYPESVMDFKQDPLSPLHDEDVFFHHPACSCSQCGKRNRQGP----PNSPA 425
           P HQYPPRQHYPES+MDFKQDPLSP HDEDVFFHHPACSCSQCGKRNRQGP    PNSPA
Sbjct: 372 PGHQYPPRQHYPESIMDFKQDPLSPSHDEDVFFHHPACSCSQCGKRNRQGPLLQAPNSPA 431

Query: 426 SNVSNPKEPIKSSTYHNETPVSVGLMASNLPCAGRFPSQDTLPHSRQPSELDSEIDGFGL 485
            NVSNPKEP KSSTYHNE PV+VGL+ASNLP AGRFPSQDTLPHSRQPSELDSEIDGFGL
Sbjct: 432 INVSNPKEPTKSSTYHNENPVTVGLVASNLPRAGRFPSQDTLPHSRQPSELDSEIDGFGL 491

Query: 486 VQPRTAAVFQRNGKSRDAIAGGAPFIVCSSCLELLKLPRKLYRLEVDWQKLQCGACSVVI 545
           VQPRTAAV QRNGKSRDAIAGGAPFIVCSSCLELLKLPRKLY+LEVDWQKLQCGACSVVI
Sbjct: 492 VQPRTAAVLQRNGKSRDAIAGGAPFIVCSSCLELLKLPRKLYKLEVDWQKLQCGACSVVI 551

Query: 546 IVKVENRKLVISVPAETKPTEVSPNDSSPKSVVNATSSIESSDNSSLKVIDTDHNKPSDD 605
           IVKV+NRKLVISVPAETKP+EVSPND SP+SVV+AT S+ESSDNSS KVIDTDHNKPSDD
Sbjct: 552 IVKVKNRKLVISVPAETKPSEVSPNDGSPQSVVDATCSVESSDNSSHKVIDTDHNKPSDD 611

Query: 606 QDSNCAKPQEEVTSSPISSKEKESPTINCDPKNLSDSDDLPLKDTPSVISSVENSDNPSH 665
           QDS+CAK Q EVTSSPISSKEKESPTINCDPKNLSDS DLP KDTPSVIS+VENSDNPSH
Sbjct: 612 QDSDCAKTQ-EVTSSPISSKEKESPTINCDPKNLSDSADLPPKDTPSVISTVENSDNPSH 671

Query: 666 DKPSEHREGTEDKQKVMVDDVTEPSELDVSFDDYANIHVSHDSVEINKEEEEEEGEEGEE 725
           DKPSEHREG+E+KQKV+VDDVTEPSELDVSFDDY+NIHVSHD+VEINKEEEEE     EE
Sbjct: 672 DKPSEHREGSENKQKVLVDDVTEPSELDVSFDDYSNIHVSHDTVEINKEEEEE-----EE 731

Query: 726 GEEDQSKIKSNQESETFFVGLSRNNLRDFSRSSEITDNGRPTVSVNGQPLPAHIVKKAEK 785
           GE+DQ+K+KSNQESETFFVGLSRNNLRDFSRSSEITDNGRPTVSVNGQPLPAHIVKKAEK
Sbjct: 732 GEDDQNKVKSNQESETFFVGLSRNNLRDFSRSSEITDNGRPTVSVNGQPLPAHIVKKAEK 791

Query: 786 HAGPILPGDYWYDYQAGFWGVMGHPCLGIIPPFIDEFTYPMSRNCAAGNTGIFVNGRELH 845
           HAGPILPGDYWYDYQAGFWGVMGHPCLGIIPPFIDEFTYPMSRNCAAGNTGIFVNGRELH
Sbjct: 792 HAGPILPGDYWYDYQAGFWGVMGHPCLGIIPPFIDEFTYPMSRNCAAGNTGIFVNGRELH 851

Query: 846 KRDLELLSSRGLPTTTNKLYRIDISGRVIDEDSGKVLYNLGKLAPTIEKVKHGFGMKVPR 905
           KRDLELLSSRGLPTTTNKLYRIDISGRVIDEDSGK LYNLGKLAPTIEKVKHGFGMKVPR
Sbjct: 852 KRDLELLSSRGLPTTTNKLYRIDISGRVIDEDSGKELYNLGKLAPTIEKVKHGFGMKVPR 908

BLAST of Cucsa.107260.1 vs. NCBI nr
Match: gi|778691018|ref|XP_011653211.1| (PREDICTED: uncharacterized protein LOC101207125 isoform X2 [Cucumis sativus])

HSP 1 Score: 1697.2 bits (4394), Expect = 0.0e+00
Identity = 824/830 (99.28%), Postives = 824/830 (99.28%), Query Frame = 1

Query: 81  MEPTVNERVMRSRRTVFSNSPIRTSDREDIDDYERKIGKETKGVWPMQRLGDKEIGSVEE 140
           MEPTVNERVMRSRRTVFSNSPIRTSDREDIDDYERKIGKETKGVWPMQRLGDKEIGSVEE
Sbjct: 1   MEPTVNERVMRSRRTVFSNSPIRTSDREDIDDYERKIGKETKGVWPMQRLGDKEIGSVEE 60

Query: 141 THSQFSEQRIENWVRRYNIEQDMNIYDSDSPSKAPYRNPTGAARTRATFEHHRVERDAFA 200
           THSQFSEQRIENWVRRYNIEQDMNIYDSDSPSKAPYRNPTGAARTRATFEHHRVERDAFA
Sbjct: 61  THSQFSEQRIENWVRRYNIEQDMNIYDSDSPSKAPYRNPTGAARTRATFEHHRVERDAFA 120

Query: 201 GYSGNSMAGAHGKGAPNFRYPGDRPSSSNLNVYYGHPEPNRNYEGPIEGFDPNRAELLRR 260
           GYSGNSMAGAHGKGAPNFRYPGDRPSSSNLNVYYGHPEPNRNYEGPIEGFDPNRAELLRR
Sbjct: 121 GYSGNSMAGAHGKGAPNFRYPGDRPSSSNLNVYYGHPEPNRNYEGPIEGFDPNRAELLRR 180

Query: 261 LDELKAEIIKSCDVGDRPRVVADRAPVDPYYSRAGYNVPMRSSTKNPQHNHDPQYFGRGS 320
           LDELKAEIIKSCDVGDRPRVVADRAPVDPYYSRAGYNVPMRSSTKNPQHNHDPQYFGRGS
Sbjct: 181 LDELKAEIIKSCDVGDRPRVVADRAPVDPYYSRAGYNVPMRSSTKNPQHNHDPQYFGRGS 240

Query: 321 GTFPATGHHQRNGEDFLHPPRHVVKDMPLYEDEFQEQMIRKTNHQPAHQYPPRQHYPESV 380
           GTFPATGHHQRNGEDFLHPPRHVVKDMPLYEDEFQEQMIRKTNHQPAHQYPPRQHYPESV
Sbjct: 241 GTFPATGHHQRNGEDFLHPPRHVVKDMPLYEDEFQEQMIRKTNHQPAHQYPPRQHYPESV 300

Query: 381 MDFKQDPLSPLHDEDVFFHHPACSCSQCGKRNRQGPPNSPASNVSNPKEPIKSSTYHNET 440
           MDFKQDPLSPLHDEDVFFHHPACSCSQCGKRNRQGPPNSPASNVSNPKEPIKSSTYHNET
Sbjct: 301 MDFKQDPLSPLHDEDVFFHHPACSCSQCGKRNRQGPPNSPASNVSNPKEPIKSSTYHNET 360

Query: 441 PVSVGLMASNLPCAGRFPSQDTLPHSRQPSELDSEIDGFGLVQPRTAAVFQRNGKSRDAI 500
           PVSVGLMASNLPCAGRFPSQDTLPHSRQPSELDSEIDGFGLVQPRTAAVFQRNGKSRDAI
Sbjct: 361 PVSVGLMASNLPCAGRFPSQDTLPHSRQPSELDSEIDGFGLVQPRTAAVFQRNGKSRDAI 420

Query: 501 AGGAPFIVCSSCLELLKLPRKLYRLEVDWQKLQCGACSVVIIVKVENRKLVISVPAETKP 560
           AGGAPFIVCSSCLELLKLPRKLYRLEVDWQKLQCGACSVVIIVKVENRKLVISVPAETKP
Sbjct: 421 AGGAPFIVCSSCLELLKLPRKLYRLEVDWQKLQCGACSVVIIVKVENRKLVISVPAETKP 480

Query: 561 TEVSPNDSSPKSVVNATSSIESSDNSSLKVIDTDHNKPSDDQDSNCAKPQEEVTSSPISS 620
           TEVSPNDSSPKSVVNATSSIESSDNSSLKVIDTDHNKPSDDQDSNCAKPQEEVTSSPISS
Sbjct: 481 TEVSPNDSSPKSVVNATSSIESSDNSSLKVIDTDHNKPSDDQDSNCAKPQEEVTSSPISS 540

Query: 621 KEKESPTINCDPKNLSDSDDLPLKDTPSVISSVENSDNPSHDKPSEHREGTEDKQKVMVD 680
           KEKESPTINCDPKNLSDSDDLPLKDTPSVISSVENSDNPSHDKPSEHREGTEDKQKVMVD
Sbjct: 541 KEKESPTINCDPKNLSDSDDLPLKDTPSVISSVENSDNPSHDKPSEHREGTEDKQKVMVD 600

Query: 681 DVTEPSELDVSFDDYANIHVSHDSVEINKEEEE------EEGEEGEEGEEDQSKIKSNQE 740
           DVTEPSELDVSFDDYANIHVSHDSVEINKEEEE      EEGEEGEEGEEDQSKIKSNQE
Sbjct: 601 DVTEPSELDVSFDDYANIHVSHDSVEINKEEEEEEGEEGEEGEEGEEGEEDQSKIKSNQE 660

Query: 741 SETFFVGLSRNNLRDFSRSSEITDNGRPTVSVNGQPLPAHIVKKAEKHAGPILPGDYWYD 800
           SETFFVGLSRNNLRDFSRSSEITDNGRPTVSVNGQPLPAHIVKKAEKHAGPILPGDYWYD
Sbjct: 661 SETFFVGLSRNNLRDFSRSSEITDNGRPTVSVNGQPLPAHIVKKAEKHAGPILPGDYWYD 720

Query: 801 YQAGFWGVMGHPCLGIIPPFIDEFTYPMSRNCAAGNTGIFVNGRELHKRDLELLSSRGLP 860
           YQAGFWGVMGHPCLGIIPPFIDEFTYPMSRNCAAGNTGIFVNGRELHKRDLELLSSRGLP
Sbjct: 721 YQAGFWGVMGHPCLGIIPPFIDEFTYPMSRNCAAGNTGIFVNGRELHKRDLELLSSRGLP 780

Query: 861 TTTNKLYRIDISGRVIDEDSGKVLYNLGKLAPTIEKVKHGFGMKVPRTLK 905
           TTTNKLYRIDISGRVIDEDSGKVLYNLGKLAPTIEKVKHGFGMKVPRTLK
Sbjct: 781 TTTNKLYRIDISGRVIDEDSGKVLYNLGKLAPTIEKVKHGFGMKVPRTLK 830

BLAST of Cucsa.107260.1 vs. NCBI nr
Match: gi|659102124|ref|XP_008451964.1| (PREDICTED: uncharacterized protein LOC103493111 isoform X3 [Cucumis melo])

HSP 1 Score: 1562.4 bits (4044), Expect = 0.0e+00
Identity = 759/828 (91.67%), Postives = 788/828 (95.17%), Query Frame = 1

Query: 81  MEPTVNERVMRSRRTVFSNSPIRTSDREDIDDYERKIGKETKGVWPMQRLGDKEIGSVEE 140
           MEPTVNERVMRSRRTVFSNSPIRTSDREDIDDYERKIGKETKGVW MQRLGDKE+GSVEE
Sbjct: 1   MEPTVNERVMRSRRTVFSNSPIRTSDREDIDDYERKIGKETKGVWTMQRLGDKEVGSVEE 60

Query: 141 THSQFSEQRIENWVRRYNIEQDMNIYDSDSPSKAPYRNPTGAARTRATFEHHRVERDAFA 200
            HSQFSEQRIENWVRRYNIEQDMNIYD DSPS APYRNP G ARTRATFEHHRVERDAF 
Sbjct: 61  PHSQFSEQRIENWVRRYNIEQDMNIYDYDSPSTAPYRNPIGVARTRATFEHHRVERDAFT 120

Query: 201 GYSGNSMAGAHGKGAPNFRYPGDRPSSSNLNVYYGHPEPNRNYEGPIEGFDPNRAELLRR 260
           GYSGNSMAGAHGKGAPNFRY  DRPSSSNL+++YGHPEPNRNYEGPIEG DPNRAELLR+
Sbjct: 121 GYSGNSMAGAHGKGAPNFRYSSDRPSSSNLDLFYGHPEPNRNYEGPIEGLDPNRAELLRK 180

Query: 261 LDELKAEIIKSCDVGDRPRVVADRAPVDPYYSRAGYNVPMRSSTKNPQHNHDPQYFGRGS 320
           LDELKA+IIKSCDV DRPRVVADRAPVDPYY RAGYNV MRSSTK+PQH + PQYFGRGS
Sbjct: 181 LDELKAQIIKSCDVVDRPRVVADRAPVDPYYGRAGYNVSMRSSTKSPQHINSPQYFGRGS 240

Query: 321 GTFPATGHHQRNGEDFLHPPRHVVKDMPLYEDEFQEQMIRKTNHQPAHQYPPRQHYPESV 380
           GTFPATG HQRNGEDFLHPPRHVVKDMPLYED+FQEQM+RKTNHQP HQYPPRQHYPES+
Sbjct: 241 GTFPATGPHQRNGEDFLHPPRHVVKDMPLYEDQFQEQMVRKTNHQPGHQYPPRQHYPESI 300

Query: 381 MDFKQDPLSPLHDEDVFFHHPACSCSQCGKRNRQGP----PNSPASNVSNPKEPIKSSTY 440
           MDFKQDPLSP HDEDVFFHHPACSCSQCGKRNRQGP    PNSPA NVSNPKEP KSSTY
Sbjct: 301 MDFKQDPLSPSHDEDVFFHHPACSCSQCGKRNRQGPLLQAPNSPAINVSNPKEPTKSSTY 360

Query: 441 HNETPVSVGLMASNLPCAGRFPSQDTLPHSRQPSELDSEIDGFGLVQPRTAAVFQRNGKS 500
           HNE PV+VGL+ASNLP AGRFPSQDTLPHSRQPSELDSEIDGFGLVQPRTAAV QRNGKS
Sbjct: 361 HNENPVTVGLVASNLPRAGRFPSQDTLPHSRQPSELDSEIDGFGLVQPRTAAVLQRNGKS 420

Query: 501 RDAIAGGAPFIVCSSCLELLKLPRKLYRLEVDWQKLQCGACSVVIIVKVENRKLVISVPA 560
           RDAIAGGAPFIVCSSCLELLKLPRKLY+LEVDWQKLQCGACSVVIIVKV+NRKLVISVPA
Sbjct: 421 RDAIAGGAPFIVCSSCLELLKLPRKLYKLEVDWQKLQCGACSVVIIVKVKNRKLVISVPA 480

Query: 561 ETKPTEVSPNDSSPKSVVNATSSIESSDNSSLKVIDTDHNKPSDDQDSNCAKPQEEVTSS 620
           ETKP+EVSPND SP+SVV+AT S+ESSDNSS KVIDTDHNKPSDDQDS+CAK Q EVTSS
Sbjct: 481 ETKPSEVSPNDGSPQSVVDATCSVESSDNSSHKVIDTDHNKPSDDQDSDCAKTQ-EVTSS 540

Query: 621 PISSKEKESPTINCDPKNLSDSDDLPLKDTPSVISSVENSDNPSHDKPSEHREGTEDKQK 680
           PISSKEKESPTINCDPKNLSDS DLP KDTPSVIS+VENSDNPSHDKPSEHREG+E+KQK
Sbjct: 541 PISSKEKESPTINCDPKNLSDSADLPPKDTPSVISTVENSDNPSHDKPSEHREGSENKQK 600

Query: 681 VMVDDVTEPSELDVSFDDYANIHVSHDSVEINKEEEEEEGEEGEEGEEDQSKIKSNQESE 740
           V+VDDVTEPSELDVSFDDY+NIHVSHD+VEINKEEEEE     EEGE+DQ+K+KSNQESE
Sbjct: 601 VLVDDVTEPSELDVSFDDYSNIHVSHDTVEINKEEEEE-----EEGEDDQNKVKSNQESE 660

Query: 741 TFFVGLSRNNLRDFSRSSEITDNGRPTVSVNGQPLPAHIVKKAEKHAGPILPGDYWYDYQ 800
           TFFVGLSRNNLRDFSRSSEITDNGRPTVSVNGQPLPAHIVKKAEKHAGPILPGDYWYDYQ
Sbjct: 661 TFFVGLSRNNLRDFSRSSEITDNGRPTVSVNGQPLPAHIVKKAEKHAGPILPGDYWYDYQ 720

Query: 801 AGFWGVMGHPCLGIIPPFIDEFTYPMSRNCAAGNTGIFVNGRELHKRDLELLSSRGLPTT 860
           AGFWGVMGHPCLGIIPPFIDEFTYPMSRNCAAGNTGIFVNGRELHKRDLELLSSRGLPTT
Sbjct: 721 AGFWGVMGHPCLGIIPPFIDEFTYPMSRNCAAGNTGIFVNGRELHKRDLELLSSRGLPTT 780

Query: 861 TNKLYRIDISGRVIDEDSGKVLYNLGKLAPTIEKVKHGFGMKVPRTLK 905
           TNKLYRIDISGRVIDEDSGK LYNLGKLAPTIEKVKHGFGMKVPRTLK
Sbjct: 781 TNKLYRIDISGRVIDEDSGKELYNLGKLAPTIEKVKHGFGMKVPRTLK 822

BLAST of Cucsa.107260.1 vs. NCBI nr
Match: gi|659102122|ref|XP_008451963.1| (PREDICTED: uncharacterized protein At5g05190-like isoform X2 [Cucumis melo])

HSP 1 Score: 1513.4 bits (3917), Expect = 0.0e+00
Identity = 735/809 (90.85%), Postives = 765/809 (94.56%), Query Frame = 1

Query: 6   KVRVVRCPRCQNLLPEPSGLSVYQCGGCGIVLKAKSKVLFNEKRDFGSSEKYENFSEQGG 65
           KVRVVRCPRCQNLLPEPSGL VYQCGGCGIVLKAKS+VLFNEKRDFGSSEKYEN SEQGG
Sbjct: 12  KVRVVRCPRCQNLLPEPSGLPVYQCGGCGIVLKAKSEVLFNEKRDFGSSEKYENLSEQGG 71

Query: 66  SSLGGVSDSERGSPSMEPTVNERVMRSRRTVFSNSPIRTSDREDIDDYERKIGKETKGVW 125
           SSLGGVSDSE  SPSMEPTVNERVMRSRRTVFSNSPIRTSDREDIDDYERKIGKETKGVW
Sbjct: 72  SSLGGVSDSEWDSPSMEPTVNERVMRSRRTVFSNSPIRTSDREDIDDYERKIGKETKGVW 131

Query: 126 PMQRLGDKEIGSVEETHSQFSEQRIENWVRRYNIEQDMNIYDSDSPSKAPYRNPTGAART 185
            MQRLGDKE+GSVEE HSQFSEQRIENWVRRYNIEQDMNIYD DSPS APYRNP G ART
Sbjct: 132 TMQRLGDKEVGSVEEPHSQFSEQRIENWVRRYNIEQDMNIYDYDSPSTAPYRNPIGVART 191

Query: 186 RATFEHHRVERDAFAGYSGNSMAGAHGKGAPNFRYPGDRPSSSNLNVYYGHPEPNRNYEG 245
           RATFEHHRVERDAF GYSGNSMAGAHGKGAPNFRY  DRPSSSNL+++YGHPEPNRNYEG
Sbjct: 192 RATFEHHRVERDAFTGYSGNSMAGAHGKGAPNFRYSSDRPSSSNLDLFYGHPEPNRNYEG 251

Query: 246 PIEGFDPNRAELLRRLDELKAEIIKSCDVGDRPRVVADRAPVDPYYSRAGYNVPMRSSTK 305
           PIEG DPNRAELLR+LDELKA+IIKSCDV DRPRVVADRAPVDPYY RAGYNV MRSSTK
Sbjct: 252 PIEGLDPNRAELLRKLDELKAQIIKSCDVVDRPRVVADRAPVDPYYGRAGYNVSMRSSTK 311

Query: 306 NPQHNHDPQYFGRGSGTFPATGHHQRNGEDFLHPPRHVVKDMPLYEDEFQEQMIRKTNHQ 365
           +PQH + PQYFGRGSGTFPATG HQRNGEDFLHPPRHVVKDMPLYED+FQEQM+RKTNHQ
Sbjct: 312 SPQHINSPQYFGRGSGTFPATGPHQRNGEDFLHPPRHVVKDMPLYEDQFQEQMVRKTNHQ 371

Query: 366 PAHQYPPRQHYPESVMDFKQDPLSPLHDEDVFFHHPACSCSQCGKRNRQGP----PNSPA 425
           P HQYPPRQHYPES+MDFKQDPLSP HDEDVFFHHPACSCSQCGKRNRQGP    PNSPA
Sbjct: 372 PGHQYPPRQHYPESIMDFKQDPLSPSHDEDVFFHHPACSCSQCGKRNRQGPLLQAPNSPA 431

Query: 426 SNVSNPKEPIKSSTYHNETPVSVGLMASNLPCAGRFPSQDTLPHSRQPSELDSEIDGFGL 485
            NVSNPKEP KSSTYHNE PV+VGL+ASNLP AGRFPSQDTLPHSRQPSELDSEIDGFGL
Sbjct: 432 INVSNPKEPTKSSTYHNENPVTVGLVASNLPRAGRFPSQDTLPHSRQPSELDSEIDGFGL 491

Query: 486 VQPRTAAVFQRNGKSRDAIAGGAPFIVCSSCLELLKLPRKLYRLEVDWQKLQCGACSVVI 545
           VQPRTAAV QRNGKSRDAIAGGAPFIVCSSCLELLKLPRKLY+LEVDWQKLQCGACSVVI
Sbjct: 492 VQPRTAAVLQRNGKSRDAIAGGAPFIVCSSCLELLKLPRKLYKLEVDWQKLQCGACSVVI 551

Query: 546 IVKVENRKLVISVPAETKPTEVSPNDSSPKSVVNATSSIESSDNSSLKVIDTDHNKPSDD 605
           IVKV+NRKLVISVPAETKP+EVSPND SP+SVV+AT S+ESSDNSS KVIDTDHNKPSDD
Sbjct: 552 IVKVKNRKLVISVPAETKPSEVSPNDGSPQSVVDATCSVESSDNSSHKVIDTDHNKPSDD 611

Query: 606 QDSNCAKPQEEVTSSPISSKEKESPTINCDPKNLSDSDDLPLKDTPSVISSVENSDNPSH 665
           QDS+CAK Q EVTSSPISSKEKESPTINCDPKNLSDS DLP KDTPSVIS+VENSDNPSH
Sbjct: 612 QDSDCAKTQ-EVTSSPISSKEKESPTINCDPKNLSDSADLPPKDTPSVISTVENSDNPSH 671

Query: 666 DKPSEHREGTEDKQKVMVDDVTEPSELDVSFDDYANIHVSHDSVEINKEEEEEEGEEGEE 725
           DKPSEHREG+E+KQKV+VDDVTEPSELDVSFDDY+NIHVSHD+VEINKEEEEE     EE
Sbjct: 672 DKPSEHREGSENKQKVLVDDVTEPSELDVSFDDYSNIHVSHDTVEINKEEEEE-----EE 731

Query: 726 GEEDQSKIKSNQESETFFVGLSRNNLRDFSRSSEITDNGRPTVSVNGQPLPAHIVKKAEK 785
           GE+DQ+K+KSNQESETFFVGLSRNNLRDFSRSSEITDNGRPTVSVNGQPLPAHIVKKAEK
Sbjct: 732 GEDDQNKVKSNQESETFFVGLSRNNLRDFSRSSEITDNGRPTVSVNGQPLPAHIVKKAEK 791

Query: 786 HAGPILPGDYWYDYQAGFWGVMGHPCLGI 811
           HAGPILPGDYWYDYQAGFWGVMGHPCL I
Sbjct: 792 HAGPILPGDYWYDYQAGFWGVMGHPCLAI 814