Cucsa.103970.1 (mRNA) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.103970.1
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Isochorismatase-like protein
Location	scaffold00927 : 2612224 .. 2613721 (+)
Sequence length	877

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GATGGTCTTAGAGGTCATGGAGCTGTTGAAGGAGCACCTTCCCCTGACTCAAGAGCCTTTCGTTTTGTCAGGTGACCTCAACATCGGCCTCGTCCTCGTCGATGTCGTTAATGGCTTCTGCACCGTCGGCGCTGGAAATTTGGTAATTCCATCTCCTCCTTACTGTTCTTCTCCTTCACCAATTTTAAATTTCGATTGATCACGGAGATTCCTATGAGTTTTGGTGATTCCGTCGATTTCATTAGATTGTGACGGCTtGTTTTTACAGGCTCCGAAACAGCATAACGAGCAAATTTCTCAAATGGTGGAGGAATCGGCAAGACTCGCCAGAGTTTtCTGCGAGAAAAaaTGGCCTATATTCGCGTTTCGTGATTCTCATCATCCGGACATTCCGGAGCCTCCCTATCCTCCTCATTGCATCGCTGGAACAGACGAATCCAAATTAGTTCCAGGTACGTACTGCTAATTTTCGTGGTTGAATCTAACTTTAGCAATTTGCAATGAAAATACAAAGCCAATTTTTGAAAACCTACCGTACTTGTTTGCAGCCCTGCAATGGCTGGAAAACGAGGCGAATGTGACGCTCAGATGCAAAGATTGCATCGATGGATTTTTAGGTTGTTTGGAAAAAGATGGCTCTAACATTTTCATCGATTGGGTGAAGAAAAATCAGATCAAAGGCGTGAGTCTCTCTAATATTCAATCGTAAATCTAAATTGGTTTAGTTACGTATTCTTTTTTtGTTtATTCAATCAGAGAGAAGTAAATTTGGATACGCTTTCTTCTTTTGAACATGAATAGATCCTAGTGCTGGGAATTTGCACGGACATATGCGTGCTGGATTTTGTATGTTCAACGTTATCAGCAAGAAATCGCGGTTTCCTTTCACCACTGGAGGATGTGATAGTATATTCCGGTGGCTGCGCCACTTATGATCTTCCCGTAGCTGTTGCCAAGACTTTGGGAGATGCCATTGCTCACCCACAGGTACCCAAATTCTTAATTCATTGTTGACTTTCATACAAATTAAGCTTCTCTTATATTTAATATTTAATATCATGAAAAAGAGCTGTTTGGGTTTTGTTTTTATCTTCTTTTTTATTTCTGGCTTTTCTTGTCTCTTCCTTAGTTCAAACCTTATTTTTATTGGAGGCAATAGGACCCGCTGAAATCACACATCACTTGCTTTACTTTATTGTTTTCCTAATTGTTGTAGTGAATTAGTACATTTTTTCTGGTTTAAACAATGTTAGTAATATTGGTTCAGGAGTTGATGCACCACGTAGGCCTGTACATAGCAAGGGGAAGAGGAGCCAAGGTGGTATCCGAGGTTTCAACTAAATCATTTTGAGGAAGCCACTTAGTTTAATTAAGCTAAGCTCCCCTCTAACATAAAGACATCTTGTGTATGGAAGTCCATTTAGTTCACTCAGTACATGTAACCGTAAACCTTCTTGGACAAATGAAGATCATACAAGTTTAGTTCTTATTTTGAGTG

mRNA sequence

GATGGTCTTAGAGGTCATGGAGCTGTTGAAGGAGCACCTTCCCCTGACTCAAGAGCCTTTCGTTTTGTCAGGTGACCTCAACATCGGCCTCGTCCTCGTCGATGTCGTTAATGGCTTCTGCACCGTCGGCGCTGGAAATTTGGCTCCGAAACAGCATAACGAGCAAATTTCTCAAATGGTGGAGGAATCGGCAAGACTCGCCAGAGTTTTCTGCGAGAAAAAATGGCCTATATTCGCGTTTCGTGATTCTCATCATCCGGACATTCCGGAGCCTCCCTATCCTCCTCATTGCATCGCTGGAACAGACGAATCCAAATTAGTTCCAGCCCTGCAATGGCTGGAAAACGAGGCGAATGTGACGCTCAGATGCAAAGATTGCATCGATGGATTTTTAGGTTGTTTGGAAAAAGATGGCTCTAACATTTTCATCGATTGGGTGAAGAAAAATCAGATCAAAGGCATCCTAGTGCTGGGAATTTGCACGGACATATGCGTGCTGGATTTTGTATGTTCAACGTTATCAGCAAGAAATCGCGGTTTCCTTTCACCACTGGAGGATGTGATAGTATATTCCGGTGGCTGCGCCACTTATGATCTTCCCGTAGCTGTTGCCAAGACTTTGGGAGATGCCATTGCTCACCCACAGGAGTTGATGCACCACGTAGGCCTGTACATAGCAAGGGGAAGAGGAGCCAAGGTGGTATCCGAGGTTTCAACTAAATCATTTTGAGGAAGCCACTTAGTTTAATTAAGCTAAGCTCCCCTCTAACATAAAGACATCTTGTGTATGGAAGTCCATTTAGTTCACTCAGTACATGTAACCGTAAACCTTCTTGGACAAATGAAGATCATACAAGTTTAGTTCTTATTTTGAGTG

Coding sequence (CDS)

ATGGTCTTAGAGGTCATGGAGCTGTTGAAGGAGCACCTTCCCCTGACTCAAGAGCCTTTCGTTTTGTCAGGTGACCTCAACATCGGCCTCGTCCTCGTCGATGTCGTTAATGGCTTCTGCACCGTCGGCGCTGGAAATTTGGCTCCGAAACAGCATAACGAGCAAATTTCTCAAATGGTGGAGGAATCGGCAAGACTCGCCAGAGTTTtCTGCGAGAAAAaaTGGCCTATATTCGCGTTTCGTGATTCTCATCATCCGGACATTCCGGAGCCTCCCTATCCTCCTCATTGCATCGCTGGAACAGACGAATCCAAATTAGTTCCAGCCCTGCAATGGCTGGAAAACGAGGCGAATGTGACGCTCAGATGCAAAGATTGCATCGATGGATTTTTAGGTTGTTTGGAAAAAGATGGCTCTAACATTTTCATCGATTGGGTGAAGAAAAATCAGATCAAAGGCATCCTAGTGCTGGGAATTTGCACGGACATATGCGTGCTGGATTTTGTATGTTCAACGTTATCAGCAAGAAATCGCGGTTTCCTTTCACCACTGGAGGATGTGATAGTATATTCCGGTGGCTGCGCCACTTATGATCTTCCCGTAGCTGTTGCCAAGACTTTGGGAGATGCCATTGCTCACCCACAGGAGTTGATGCACCACGTAGGCCTGTACATAGCAAGGGGAAGAGGAGCCAAGGTGGTATCCGAGGTTTCAACTAAATCATTTTGA

Protein sequence

MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMVEESARLARVFCEKKWPIFAFRDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVTLRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGFLSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHVGLYIARGRGAKVVSEVSTKSF*

Homology

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BLAST of Cucsa.103970.1 vs. Swiss-Prot
Match: NIC1_ARATH (Nicotinamidase 1 OS=Arabidopsis thaliana GN=NIC1 PE=1 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 4.2e-95
Identity = 159/235 (67.66%), Postives = 202/235 (85.96%), Query Frame = 1


Query: 5   VMELLKEHLPLTQE-PFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMVEES 64
           + + LK+ +P+ +E P +L+ D ++GLV+VDVVNGFCT+G+GN+AP +HNEQIS+MVEES
Sbjct: 7   IFDQLKKQIPVDEEEPLILNRDSSVGLVIVDVVNGFCTIGSGNMAPTKHNEQISKMVEES 66

Query: 65  ARLARVFCEKKWPIFAFRDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVTLRC 124
           A+LAR FC++KWP+ AF DSHHPDIPE PYPPHCI GT+ES+LVPAL+WLE+E   TLR 
Sbjct: 67  AKLAREFCDRKWPVLAFIDSHHPDIPERPYPPHCIIGTEESELVPALKWLESEDCATLRR 126

Query: 125 KDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGFLSP 184
           KDCI+GF+G +E DGSN+F+DWVK+ QIK I+V+GICTDICV DFV + LSARN G LSP
Sbjct: 127 KDCINGFVGSMESDGSNVFVDWVKEKQIKVIVVVGICTDICVFDFVATALSARNHGVLSP 186

Query: 185 LEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHVGLYIARGRGAKVVSEVS 239
           +EDV+VYS GCAT+DLP+ VAK +  A AHPQELMHHVGLY+A+GRGA+VVS++S
Sbjct: 187 VEDVVVYSRGCATFDLPLHVAKDIKGAQAHPQELMHHVGLYMAKGRGAQVVSKIS 241

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BLAST of Cucsa.103970.1 vs. TrEMBL
Match: A0A0A0KG59_CUCSA (Catalytic OS=Cucumis sativus GN=Csa_6G498960 PE=4 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 2.8e-138
Identity = 242/242 (100.00%), Postives = 242/242 (100.00%), Query Frame = 1


Query: 1   MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMV 60
           MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMV
Sbjct: 1   MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMV 60

Query: 61  EESARLARVFCEKKWPIFAFRDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVT 120
           EESARLARVFCEKKWPIFAFRDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVT
Sbjct: 61  EESARLARVFCEKKWPIFAFRDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVT 120

Query: 121 LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGF 180
           LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGF
Sbjct: 121 LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGF 180

Query: 181 LSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHVGLYIARGRGAKVVSEVSTK 240
           LSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHVGLYIARGRGAKVVSEVSTK
Sbjct: 181 LSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHVGLYIARGRGAKVVSEVSTK 240

Query: 241 SF 243
           SF
Sbjct: 241 SF 242

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BLAST of Cucsa.103970.1 vs. TrEMBL
Match: F6I2H0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_00s1364g00010 PE=4 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 6.8e-108
Identity = 187/238 (78.57%), Postives = 213/238 (89.50%), Query Frame = 1


Query: 1   MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMV 60
           MV + +++L+  LP+ QE  VLSG++  GLVLVDVVNGFCTVGAGNLAP + ++QIS MV
Sbjct: 1   MVSKTIDMLRNELPVQQESLVLSGEVPTGLVLVDVVNGFCTVGAGNLAPVRPDKQISGMV 60

Query: 61  EESARLARVFCEKKWPIFAFRDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVT 120
           +ES RLARVFCEKKWP+FAF DSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENE NVT
Sbjct: 61  DESVRLARVFCEKKWPVFAFLDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEPNVT 120

Query: 121 LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGF 180
           +RCKDCIDGFLG +EKDGSN+F+DWVK NQIK ILV+GICTDICVLDFVCST+SARNRGF
Sbjct: 121 IRCKDCIDGFLGAIEKDGSNVFVDWVKTNQIKIILVVGICTDICVLDFVCSTISARNRGF 180

Query: 181 LSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHVGLYIARGRGAKVVSEVS 239
            + LEDVIVYS GCAT+DLP  VA+T+  AIAHPQELMHH+GLY+A+GRGAKVVSEVS
Sbjct: 181 FTSLEDVIVYSHGCATFDLPDDVARTIPGAIAHPQELMHHIGLYMAKGRGAKVVSEVS 238

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BLAST of Cucsa.103970.1 vs. TrEMBL
Match: A0A067KD75_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_18704 PE=4 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 8.3e-106
Identity = 181/233 (77.68%), Postives = 208/233 (89.27%), Query Frame = 1


Query: 6   MELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMVEESAR 65
           +ELLK+ LP+ Q   +L+ D+  GLVLVD+VNGFCTVGAGNLAPKQ ++QIS MVEES +
Sbjct: 8   IELLKQELPVEQGSLLLNADVKAGLVLVDIVNGFCTVGAGNLAPKQPDKQISTMVEESVK 67

Query: 66  LARVFCEKKWPIFAFRDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVTLRCKD 125
           LAR FC+KKWP+FAF DSHHPDIPE PYPPHCIAGTDE++LVP LQWLENE+NVTLRCKD
Sbjct: 68  LARAFCDKKWPVFAFIDSHHPDIPEHPYPPHCIAGTDEARLVPELQWLENESNVTLRCKD 127

Query: 126 CIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGFLSPLE 185
           CIDGFLG +EKDGSN+F++W+K NQIK ILV+GICTDICVLDFVCSTLSARNRGFLSPLE
Sbjct: 128 CIDGFLGSIEKDGSNLFVEWIKTNQIKFILVVGICTDICVLDFVCSTLSARNRGFLSPLE 187

Query: 186 DVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHVGLYIARGRGAKVVSEVS 239
           DV VYS  CAT+DLPV VA+T+ D  AHPQELMHH+GLY+A+GRGAKVVSEVS
Sbjct: 188 DVFVYSQACATFDLPVHVARTIKDVTAHPQELMHHIGLYMAKGRGAKVVSEVS 240

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BLAST of Cucsa.103970.1 vs. TrEMBL
Match: W9SD83_9ROSA (Putative isochorismatase family protein pncA OS=Morus notabilis GN=L484_026771 PE=4 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 1.8e-105
Identity = 177/238 (74.37%), Postives = 208/238 (87.39%), Query Frame = 1


Query: 1   MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMV 60
           M+ + ++LL+E LP+  EPF LSGD+  GLVLVD+VNGFCTVGAGN+AP+Q ++QIS+MV
Sbjct: 1   MISKTIDLLQEELPVKDEPFSLSGDVKTGLVLVDIVNGFCTVGAGNMAPRQPDKQISEMV 60

Query: 61  EESARLARVFCEKKWPIFAFRDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVT 120
           +ES RLAR FCE+KWP+FAF DSH P+IPE PYPPHC AGTDE++LVPALQWLENE NVT
Sbjct: 61  DESVRLARAFCERKWPVFAFMDSHDPNIPEHPYPPHCFAGTDEARLVPALQWLENEPNVT 120

Query: 121 LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGF 180
           LRCKDCIDGFLG +EKDGSN+F+DW+K NQIK ILV+GICTDICVLDF+CS  S RNR  
Sbjct: 121 LRCKDCIDGFLGSIEKDGSNVFVDWIKNNQIKAILVVGICTDICVLDFICSASSVRNRRL 180

Query: 181 LSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHVGLYIARGRGAKVVSEVS 239
           L+PLEDVIVYSGGCATYD+PV VA+   D IAHPQ LMHH+GLY+A+GRGAKVVSEVS
Sbjct: 181 LAPLEDVIVYSGGCATYDIPVHVARAAKDVIAHPQNLMHHIGLYMAQGRGAKVVSEVS 238

[Close]

BLAST of Cucsa.103970.1 vs. TrEMBL
Match: B9T6U0_RICCO (Catalytic, putative OS=Ricinus communis GN=RCOM_0270690 PE=4 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 3.2e-105
Identity = 179/241 (74.27%), Postives = 209/241 (86.72%), Query Frame = 1


Query: 1   MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMV 60
           MV   +ELL++ LP+ Q+  +L+GD+  GLVLVD+VNGFCTVGAGNLAPKQ +  IS MV
Sbjct: 1   MVSSTVELLRQELPVQQDSLLLNGDVKTGLVLVDIVNGFCTVGAGNLAPKQPDRLISTMV 60

Query: 61  EESARLARVFCEKKWPIFAFRDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVT 120
           EESARLAR FC+ KWP+FAF D+HHPDIPEPP+PPHC+AGTDE++LVP LQWLENEANVT
Sbjct: 61  EESARLARTFCDSKWPVFAFLDTHHPDIPEPPFPPHCLAGTDEARLVPELQWLENEANVT 120

Query: 121 LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGF 180
           LRCKDCIDGFLG +EKDGSN+F+DW++ NQIK ILV+GICTDICVLDFV S LSARNRG 
Sbjct: 121 LRCKDCIDGFLGSIEKDGSNLFVDWIRNNQIKVILVIGICTDICVLDFVSSALSARNRGL 180

Query: 181 LSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHVGLYIARGRGAKVVSEVSTK 240
           L+PLEDVIVYS  CATYDLPV VA+   DA AHPQELMHH+GLY+A+GRGAK+VSEVS  
Sbjct: 181 LAPLEDVIVYSQACATYDLPVHVARASKDAFAHPQELMHHIGLYMAKGRGAKIVSEVSVS 240

Query: 241 S 242
           +
Sbjct: 241 A 241

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BLAST of Cucsa.103970.1 vs. TAIR10
Match: AT2G22570.1 (AT2G22570.1 nicotinamidase 1)

HSP 1 Score: 349.0 bits (894), Expect = 2.4e-96
Identity = 159/235 (67.66%), Postives = 202/235 (85.96%), Query Frame = 1


Query: 5   VMELLKEHLPLTQE-PFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMVEES 64
           + + LK+ +P+ +E P +L+ D ++GLV+VDVVNGFCT+G+GN+AP +HNEQIS+MVEES
Sbjct: 7   IFDQLKKQIPVDEEEPLILNRDSSVGLVIVDVVNGFCTIGSGNMAPTKHNEQISKMVEES 66

Query: 65  ARLARVFCEKKWPIFAFRDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVTLRC 124
           A+LAR FC++KWP+ AF DSHHPDIPE PYPPHCI GT+ES+LVPAL+WLE+E   TLR 
Sbjct: 67  AKLAREFCDRKWPVLAFIDSHHPDIPERPYPPHCIIGTEESELVPALKWLESEDCATLRR 126

Query: 125 KDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGFLSP 184
           KDCI+GF+G +E DGSN+F+DWVK+ QIK I+V+GICTDICV DFV + LSARN G LSP
Sbjct: 127 KDCINGFVGSMESDGSNVFVDWVKEKQIKVIVVVGICTDICVFDFVATALSARNHGVLSP 186

Query: 185 LEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHVGLYIARGRGAKVVSEVS 239
           +EDV+VYS GCAT+DLP+ VAK +  A AHPQELMHHVGLY+A+GRGA+VVS++S
Sbjct: 187 VEDVVVYSRGCATFDLPLHVAKDIKGAQAHPQELMHHVGLYMAKGRGAQVVSKIS 241

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BLAST of Cucsa.103970.1 vs. NCBI nr
Match: gi|449451387|ref|XP_004143443.1| (PREDICTED: nicotinamidase 1 [Cucumis sativus])

HSP 1 Score: 499.2 bits (1284), Expect = 4.1e-138
Identity = 242/242 (100.00%), Postives = 242/242 (100.00%), Query Frame = 1


Query: 1   MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMV 60
           MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMV
Sbjct: 1   MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMV 60

Query: 61  EESARLARVFCEKKWPIFAFRDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVT 120
           EESARLARVFCEKKWPIFAFRDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVT
Sbjct: 61  EESARLARVFCEKKWPIFAFRDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVT 120

Query: 121 LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGF 180
           LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGF
Sbjct: 121 LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGF 180

Query: 181 LSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHVGLYIARGRGAKVVSEVSTK 240
           LSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHVGLYIARGRGAKVVSEVSTK
Sbjct: 181 LSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHVGLYIARGRGAKVVSEVSTK 240

Query: 241 SF 243
           SF
Sbjct: 241 SF 242

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BLAST of Cucsa.103970.1 vs. NCBI nr
Match: gi|659079951|ref|XP_008440534.1| (PREDICTED: uncharacterized protein LOC103484929 [Cucumis melo])

HSP 1 Score: 476.1 bits (1224), Expect = 3.7e-131
Identity = 229/240 (95.42%), Postives = 235/240 (97.92%), Query Frame = 1


Query: 1   MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMV 60
           MVLEVMELLKEHLP+TQEPFVLSGD+N GLVLVDVVNGFCTVGAGNLAPKQHN+QISQMV
Sbjct: 1   MVLEVMELLKEHLPVTQEPFVLSGDVNTGLVLVDVVNGFCTVGAGNLAPKQHNKQISQMV 60

Query: 61  EESARLARVFCEKKWPIFAFRDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVT 120
           EESARLARVFCEKKWPIFAF DSHHPDIPE PYPPHCIAGTDESKLVPALQWLENEANVT
Sbjct: 61  EESARLARVFCEKKWPIFAFLDSHHPDIPEHPYPPHCIAGTDESKLVPALQWLENEANVT 120

Query: 121 LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGF 180
           LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIK ILVLGICTDICVLDFVCSTLSARNRGF
Sbjct: 121 LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKNILVLGICTDICVLDFVCSTLSARNRGF 180

Query: 181 LSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHVGLYIARGRGAKVVSEVSTK 240
           LSPLEDVIVYSGGCATYDLPV+VAKTLGDAIAHPQELMHH+GLY+ARGRGAKVVSEVS K
Sbjct: 181 LSPLEDVIVYSGGCATYDLPVSVAKTLGDAIAHPQELMHHIGLYMARGRGAKVVSEVSIK 240

[Close]

BLAST of Cucsa.103970.1 vs. NCBI nr
Match: gi|225468460|ref|XP_002266720.1| (PREDICTED: uncharacterized protein LOC100247829 [Vitis vinifera])

HSP 1 Score: 398.3 bits (1022), Expect = 9.7e-108
Identity = 187/238 (78.57%), Postives = 213/238 (89.50%), Query Frame = 1


Query: 1   MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMV 60
           MV + +++L+  LP+ QE  VLSG++  GLVLVDVVNGFCTVGAGNLAP + ++QIS MV
Sbjct: 1   MVSKTIDMLRNELPVQQESLVLSGEVPTGLVLVDVVNGFCTVGAGNLAPVRPDKQISGMV 60

Query: 61  EESARLARVFCEKKWPIFAFRDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVT 120
           +ES RLARVFCEKKWP+FAF DSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENE NVT
Sbjct: 61  DESVRLARVFCEKKWPVFAFLDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEPNVT 120

Query: 121 LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGF 180
           +RCKDCIDGFLG +EKDGSN+F+DWVK NQIK ILV+GICTDICVLDFVCST+SARNRGF
Sbjct: 121 IRCKDCIDGFLGAIEKDGSNVFVDWVKTNQIKIILVVGICTDICVLDFVCSTISARNRGF 180

Query: 181 LSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHVGLYIARGRGAKVVSEVS 239
            + LEDVIVYS GCAT+DLP  VA+T+  AIAHPQELMHH+GLY+A+GRGAKVVSEVS
Sbjct: 181 FTSLEDVIVYSHGCATFDLPDDVARTIPGAIAHPQELMHHIGLYMAKGRGAKVVSEVS 238

[Close]

BLAST of Cucsa.103970.1 vs. NCBI nr
Match: gi|296088949|emb|CBI38515.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 394.0 bits (1011), Expect = 1.8e-106
Identity = 185/231 (80.09%), Postives = 208/231 (90.04%), Query Frame = 1


Query: 8   LLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMVEESARLA 67
           +L+  LP+ QE  VLSG++  GLVLVDVVNGFCTVGAGNLAP + ++QIS MV+ES RLA
Sbjct: 1   MLRNELPVQQESLVLSGEVPTGLVLVDVVNGFCTVGAGNLAPVRPDKQISGMVDESVRLA 60

Query: 68  RVFCEKKWPIFAFRDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVTLRCKDCI 127
           RVFCEKKWP+FAF DSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENE NVT+RCKDCI
Sbjct: 61  RVFCEKKWPVFAFLDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEPNVTIRCKDCI 120

Query: 128 DGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGFLSPLEDV 187
           DGFLG +EKDGSN+F+DWVK NQIK ILV+GICTDICVLDFVCST+SARNRGF + LEDV
Sbjct: 121 DGFLGAIEKDGSNVFVDWVKTNQIKIILVVGICTDICVLDFVCSTISARNRGFFTSLEDV 180

Query: 188 IVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHVGLYIARGRGAKVVSEVS 239
           IVYS GCAT+DLP  VA+T+  AIAHPQELMHH+GLY+A+GRGAKVVSEVS
Sbjct: 181 IVYSHGCATFDLPDDVARTIPGAIAHPQELMHHIGLYMAKGRGAKVVSEVS 231

[Close]

BLAST of Cucsa.103970.1 vs. NCBI nr
Match: gi|694376990|ref|XP_009364818.1| (PREDICTED: uncharacterized protein LOC103954717 [Pyrus x bretschneideri])

HSP 1 Score: 394.0 bits (1011), Expect = 1.8e-106
Identity = 185/238 (77.73%), Postives = 211/238 (88.66%), Query Frame = 1


Query: 1   MVLEVMELLKEHLPLTQEPFVLSGDLNIGLVLVDVVNGFCTVGAGNLAPKQHNEQISQMV 60
           MV + ++LLKE LP+ QEP +L+G    GLVLVDVVNGFCTVGAGNLAP+Q ++QIS+MV
Sbjct: 1   MVPQTIDLLKEQLPVHQEPLLLTGHTTTGLVLVDVVNGFCTVGAGNLAPRQPDKQISEMV 60

Query: 61  EESARLARVFCEKKWPIFAFRDSHHPDIPEPPYPPHCIAGTDESKLVPALQWLENEANVT 120
           EES RLARVFC+KKWP+FAF DSHHPDIPE PYPPHCI GTDES LVPALQWLENE NVT
Sbjct: 61  EESVRLARVFCDKKWPVFAFLDSHHPDIPEDPYPPHCIVGTDESNLVPALQWLENEPNVT 120

Query: 121 LRCKDCIDGFLGCLEKDGSNIFIDWVKKNQIKGILVLGICTDICVLDFVCSTLSARNRGF 180
           LR KDCIDGFLG +EKDGSN+FI+WV+ NQIK ILV+GICTDICVLDFVC+TLSARNR  
Sbjct: 121 LRRKDCIDGFLGSIEKDGSNMFINWVQSNQIKAILVVGICTDICVLDFVCTTLSARNRRL 180

Query: 181 LSPLEDVIVYSGGCATYDLPVAVAKTLGDAIAHPQELMHHVGLYIARGRGAKVVSEVS 239
           L+PLEDVIVYS GCAT+D+PV +AKT  D IAHPQELMHH+GLY+++GRGAKVVSEVS
Sbjct: 181 LAPLEDVIVYSHGCATFDIPVEIAKTAEDVIAHPQELMHHIGLYMSKGRGAKVVSEVS 238

The following BLAST results are available for this feature:

BLAST of Cucsa.103970.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
NIC1_ARATH	4.2e-95	67.66	Nicotinamidase 1 OS=Arabidopsis thaliana GN=NIC1 PE=1 SV=1	[more]

BLAST of Cucsa.103970.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KG59_CUCSA	2.8e-138	100.00	Catalytic OS=Cucumis sativus GN=Csa_6G498960 PE=4 SV=1	[more]
F6I2H0_VITVI	6.8e-108	78.57	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_00s1364g00010 PE=4 SV=...	[more]
A0A067KD75_JATCU	8.3e-106	77.68	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_18704 PE=4 SV=1	[more]
W9SD83_9ROSA	1.8e-105	74.37	Putative isochorismatase family protein pncA OS=Morus notabilis GN=L484_026771 P...	[more]
B9T6U0_RICCO	3.2e-105	74.27	Catalytic, putative OS=Ricinus communis GN=RCOM_0270690 PE=4 SV=1	[more]

BLAST of Cucsa.103970.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G22570.1	2.4e-96	67.66	nicotinamidase 1	[more]

BLAST of Cucsa.103970.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449451387\|ref\|XP_004143443.1\|	4.1e-138	100.00	PREDICTED: nicotinamidase 1 [Cucumis sativus]	[more]
gi\|659079951\|ref\|XP_008440534.1\|	3.7e-131	95.42	PREDICTED: uncharacterized protein LOC103484929 [Cucumis melo]	[more]
gi\|225468460\|ref\|XP_002266720.1\|	9.7e-108	78.57	PREDICTED: uncharacterized protein LOC100247829 [Vitis vinifera]	[more]
gi\|296088949\|emb\|CBI38515.3\|	1.8e-106	80.09	unnamed protein product [Vitis vinifera]	[more]
gi\|694376990\|ref\|XP_009364818.1\|	1.8e-106	77.73	PREDICTED: uncharacterized protein LOC103954717 [Pyrus x bretschneideri]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR000868	Isochorismatase-like

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
biological_process	GO:0006769	nicotinamide metabolic process
biological_process	GO:0046497	nicotinate nucleotide metabolic process
biological_process	GO:0019365	pyridine nucleotide salvage
biological_process	GO:0009737	response to abscisic acid
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0008936	nicotinamidase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsa.103970	Cucsa.103970	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsa.103970.1	Cucsa.103970.1-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.103970.1.five_prime_UTR.1	Cucsa.103970.1.five_prime_UTR.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.103970.1.CDS.1	Cucsa.103970.1.CDS.1	CDS
Cucsa.103970.1.CDS.2	Cucsa.103970.1.CDS.2	CDS
Cucsa.103970.1.CDS.3	Cucsa.103970.1.CDS.3	CDS
Cucsa.103970.1.CDS.4	Cucsa.103970.1.CDS.4	CDS
Cucsa.103970.1.CDS.5	Cucsa.103970.1.CDS.5	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.103970.1.three_prime_UTR.1	Cucsa.103970.1.three_prime_UTR.1	three_prime_UTR

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000868	Isochorismatase-like	GENE3D	G3DSA:3.40.50.850		coord: 30..235 score: 2.0
IPR000868	Isochorismatase-like	PFAM	PF00857	Isochorismatase	coord: 30..199 score: 3.7
IPR000868	Isochorismatase-like	unknown	SSF52499	Isochorismatase-like hydrolases	coord: 30..235 score: 3.14
None	No IPR available	PANTHER	PTHR11080	PYRAZINAMIDASE/NICOTINAMIDASE	coord: 2..237 score: 6.5E
None	No IPR available	PANTHER	PTHR11080:SF32	EXPRESSED PROTEIN	coord: 2..237 score: 6.5E