Cucsa.102750.1 (mRNA) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.102750.1
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Cytochrome P450
Location	scaffold00927 : 1719027 .. 1720997 (-)
Sequence length	1402

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCTCCGTCTCTCCCCATCATCGGCCACCTCCATTATATCAAAATCCCCCTCCACCGAACTTTACACAAACTCTCTGCAAAATACGGACCATTAATCTCTCTCCGGTTTGGATCTCGCCTCGTTGTGGTCGTCTCGTCATTGCCAGCAGTGGAGGAGTGCTTCACTAAGAACGACATCGTTTTAGCCAACCGTCCTCGGTTGCTATTCGGAAAACACATCGGCTACAACCACACTACGATGGTAGGGTCACCTTACGGAGACCACTGGCGGAACCTCCGCCGAATCGGAGCCATCGAGATCTTTTCAGCGTCTCGCCTCAATAAATTCGCAGGAACCCGGAAGGATGAAGTGGAACGGTTACTGAAGAAGCTATCGAGGAATTCGATGCATGGATACTCGAAAGTAGAGATGCAATCGTCGATTTCGGAACTCACGTTTAACATATCGATGAGAATGGCGGCCGGAAAGAGGTATTTCGGTGATGAAGTGAGGGATGTGGAGGAAGCGAGACAGTCGAGAGAGCTGATTAAACAGATTGTGTCGATGGGAGGAGTATCGAATCCGGGAGATTTCATTCCGATGATGAATTGGGTCCCGAATGGATATAAGAGGAAGGTTTCGAGACTTGGGAAAAGGATGGACAGGTTTTTGCAGGGATTGATTGATGAACATCGGAGCAATAAGGAAGAGGAAAGGAACACAATGATCGATCATTTGCTATCTCTGCAGGAAACGGAGCCTGAGTACTATGGAGATGAAATAATCAAAGGAATTGTTCTGGTAATTAATCTCCATAATTTCTCACTCATTTTCAATCTTCATTGCCATTATTCTCAAGAATTTTGCTCTTTATCCATCTCTTTTGGGTAATCCCCGAAATATATATAAGAACATATAATAGGACAGAATGGCAACGAAGATTCAAAGTGGTGGCCTTGCGGCCTGGGGGACCCCTATCGTCTCTCCTCTACATTTAGCATTTCCCCTCAAACTATTTTTAAAAGCATTTTGGGGCAAAGATAGAAAAATGCTCTAATATTTTCATGCACAAACTGTTTGCTTTGAGTGAAAAAAGAAAAAGTCACGCACCTTTGACGTTGGGGAAGCACTTGGCCATATTGTAGAGAGAAAATTACCATTAATTAATTTTCATATTTAATTATGGCTTACAAGCTTTAGGGTTAGGAGTAACATGAAAGAGTGTTTGCTTTGAAATAACATTGTTTAGATTTTTTTTACTAATTTTTCCATATTTAGTCCTAATTATTGACTTGGTAGGATTTGTTTTTGTTTTGTGATATCTGTTTTGTGTGTATTATATCAAACTCTAAACAAAAATAAGCGTACAGGTGTTACTACTAGCTGGAACAGACACATCAGCTGTGACAATTGAATGGGCTTTAGCTCACTTGCTCAACAATCCAGAGGTACTAAAAAAGGCTAGAGAAGAGTTGGACAATCAAATTGGAGAAAAACAACTAGCAGAAGAATTAGACGTCTCTAAGTTACCATATCTTCAAGGAATCATTTATGAGACTCTTCGATTGAACCCAGCTGCTCCAATGCTAGTGCCACATCTAACATCCAACAATTGTACAATCAGTGAGTACGAAATACCACGTGATACGATTGTATTGGTTAATGCATGGGCTATCCACAGAGACCCTAGTCAATGGGAAGAGCCCACATTATTCAAGCCAGAAAGACACCAAAACTTAGAATCAAGTGACCATCATCAAATCCCAAAGATGATTCCATTTGGAGTCGGACGACGAGCTTGCCCCGGCTCCAGCATGGCTCAACGAGTGGTGGGACTGACTCTAGCGACTCTGATTCAATGCTACGAGTGGGAGAGGATTGGGGAAGAGAAAGTGGACATGACGGAAGGAAGAGGCGTCACTATGCCCAAGGTGGTGCCATTGGAAGCTATGTGCAAAGCTCGTCCTATCGTCCACAACATTTTTAACTGA

mRNA sequence

TCCTCCGTCTCTCCCCATCATCGGCCACCTCCATTATATCAAAATCCCCCTCCACCGAACTTTACACAAACTCTCTGCAAAATACGGACCATTAATCTCTCTCCGGTTTGGATCTCGCCTCGTTGTGGTCGTCTCGTCATTGCCAGCAGTGGAGGAGTGCTTCACTAAGAACGACATCGTTTTAGCCAACCGTCCTCGGTTGCTATTCGGAAAACACATCGGCTACAACCACACTACGATGGTAGGGTCACCTTACGGAGACCACTGGCGGAACCTCCGCCGAATCGGAGCCATCGAGATCTTTTCAGCGTCTCGCCTCAATAAATTCGCAGGAACCCGGAAGGATGAAGTGGAACGGTTACTGAAGAAGCTATCGAGGAATTCGATGCATGGATACTCGAAAGTAGAGATGCAATCGTCGATTTCGGAACTCACGTTTAACATATCGATGAGAATGGCGGCCGGAAAGAGGTATTTCGGTGATGAAGTGAGGGATGTGGAGGAAGCGAGACAGTCGAGAGAGCTGATTAAACAGATTGTGTCGATGGGAGGAGTATCGAATCCGGGAGATTTCATTCCGATGATGAATTGGGTCCCGAATGGATATAAGAGGAAGGTTTCGAGACTTGGGAAAAGGATGGACAGGTTTTTGCAGGGATTGATTGATGAACATCGGAGCAATAAGGAAGAGGAAAGGAACACAATGATCGATCATTTGCTATCTCTGCAGGAAACGGAGCCTGAGTACTATGGAGATGAAATAATCAAAGGAATTGTTCTGGTGTTACTACTAGCTGGAACAGACACATCAGCTGTGACAATTGAATGGGCTTTAGCTCACTTGCTCAACAATCCAGAGGTACTAAAAAAGGCTAGAGAAGAGTTGGACAATCAAATTGGAGAAAAACAACTAGCAGAAGAATTAGACGTCTCTAAGTTACCATATCTTCAAGGAATCATTTATGAGACTCTTCGATTGAACCCAGCTGCTCCAATGCTAGTGCCACATCTAACATCCAACAATTGTACAATCAGTGAGTACGAAATACCACGTGATACGATTGTATTGGTTAATGCATGGGCTATCCACAGAGACCCTAGTCAATGGGAAGAGCCCACATTATTCAAGCCAGAAAGACACCAAAACTTAGAATCAAGTGACCATCATCAAATCCCAAAGATGATTCCATTTGGAGTCGGACGACGAGCTTGCCCCGGCTCCAGCATGGCTCAACGAGTGGTGGGACTGACTCTAGCGACTCTGATTCAATGCTACGAGTGGGAGAGGATTGGGGAAGAGAAAGTGGACATGACGGAAGGAAGAGGCGTCACTATGCCCAAGGTGGTGCCATTGGAAGCTATGTGCAAAGCTCGTCCTATCGTCCACAACATTTTTAACTGA

Coding sequence (CDS)

TCCTCCGTCTCTCCCCATCATCGGCCACCTCCATTATATCAAAATCCCCCTCCACCGAACTTTACACAAACTCTCTGCAAAATACGGACCATTAATCTCTCTCCGGTTTGGATCTCGCCTCGTTGTGGTCGTCTCGTCATTGCCAGCAGTGGAGGAGTGCTTCACTAAGAACGACATCGTTTTAGCCAACCGTCCTCGGTTGCTATTCGGAAAACACATCGGCTACAACCACACTACGATGGTAGGGTCACCTTACGGAGACCACTGGCGGAACCTCCGCCGAATCGGAGCCATCGAGATCTTTTCAGCGTCTCGCCTCAATAAATTCGCAGGAACCCGGAAGGATGAAGTGGAACGGTTACTGAAGAAGCTATCGAGGAATTCGATGCATGGATACTCGAAAGTAGAGATGCAATCGTCGATTTCGGAACTCACGTTTAACATATCGATGAGAATGGCGGCCGGAAAGAGGTATTTCGGTGATGAAGTGAGGGATGTGGAGGAAGCGAGACAGTCGAGAGAGCTGATTAAACAGATTGTGTCGATGGGAGGAGTATCGAATCCGGGAGATTTCATTCCGATGATGAATTGGGTCCCGAATGGATATAAGAGGAAGGTTTCGAGACTTGGGAAAAGGATGGACAGGTTTTTGCAGGGATTGATTGATGAACATCGGAGCAATAAGGAAGAGGAAAGGAACACAATGATCGATCATTTGCTATCTCTGCAGGAAACGGAGCCTGAGTACTATGGAGATGAAATAATCAAAGGAATTGTTCTGGTGTTACTACTAGCTGGAACAGACACATCAGCTGTGACAATTGAATGGGCTTTAGCTCACTTGCTCAACAATCCAGAGGTACTAAAAAAGGCTAGAGAAGAGTTGGACAATCAAATTGGAGAAAAACAACTAGCAGAAGAATTAGACGTCTCTAAGTTACCATATCTTCAAGGAATCATTTATGAGACTCTTCGATTGAACCCAGCTGCTCCAATGCTAGTGCCACATCTAACATCCAACAATTGTACAATCAGTGAGTACGAAATACCACGTGATACGATTGTATTGGTTAATGCATGGGCTATCCACAGAGACCCTAGTCAATGGGAAGAGCCCACATTATTCAAGCCAGAAAGACACCAAAACTTAGAATCAAGTGACCATCATCAAATCCCAAAGATGATTCCATTTGGAGTCGGACGACGAGCTTGCCCCGGCTCCAGCATGGCTCAACGAGTGGTGGGACTGACTCTAGCGACTCTGATTCAATGCTACGAGTGGGAGAGGATTGGGGAAGAGAAAGTGGACATGACGGAAGGAAGAGGCGTCACTATGCCCAAGGTGGTGCCATTGGAAGCTATGTGCAAAGCTCGTCCTATCGTCCACAACATTTTTAACTGA

Protein sequence

PPSLPIIGHLHYIKIPLHRTLHKLSAKYGPLISLRFGSRLVVVVSSLPAVEECFTKNDIVLANRPRLLFGKHIGYNHTTMVGSPYGDHWRNLRRIGAIEIFSASRLNKFAGTRKDEVERLLKKLSRNSMHGYSKVEMQSSISELTFNISMRMAAGKRYFGDEVRDVEEARQSRELIKQIVSMGGVSNPGDFIPMMNWVPNGYKRKVSRLGKRMDRFLQGLIDEHRSNKEEERNTMIDHLLSLQETEPEYYGDEIIKGIVLVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDNQIGEKQLAEELDVSKLPYLQGIIYETLRLNPAAPMLVPHLTSNNCTISEYEIPRDTIVLVNAWAIHRDPSQWEEPTLFKPERHQNLESSDHHQIPKMIPFGVGRRACPGSSMAQRVVGLTLATLIQCYEWERIGEEKVDMTEGRGVTMPKVVPLEAMCKARPIVHNIFN*

Homology

[Close]

BLAST of Cucsa.102750.1 vs. Swiss-Prot
Match: C81E8_MEDTR (Cytochrome P450 81E8 OS=Medicago truncatula GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 6.8e-158
Identity = 268/461 (58.13%), Postives = 355/461 (77.01%), Query Frame = 1


Query: 1   PPSLPIIGHLHYIKIPLHRTLHKLSAKYGPLISLRFGSRLVVVVSSLPAVEECFTKNDIV 60
           P  LPIIG+LH +K PLH T H LS KYG + SL FGSRLVVVVSSL   +ECFTKNDIV
Sbjct: 36  PQCLPIIGNLHQLKQPLHHTFHTLSQKYGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIV 95

Query: 61  LANRPRLLFGKHIGYNHTTMVGSPYGDHWRNLRRIGAIEIFSASRLNKFAGTRKDEVERL 120
           LANRP  L GK+IGYN+TT+  SPYGDHWRNLRRI +IEI S+ RLN F   R+DE+ RL
Sbjct: 96  LANRPHFLTGKYIGYNNTTVAQSPYGDHWRNLRRILSIEILSSHRLNSFLEIRRDEIMRL 155

Query: 121 LKKLSRNSMHGYSKVEMQSSISELTFNISMRMAAGKRYFGD--EVRDVEEARQSRELIKQ 180
           ++KL++ S +G+++VE++   SE+TFN  MRM +GKRY+G+  +V DVEEAR  R +IK+
Sbjct: 156 IQKLAQKSYNGFTEVELRPMFSEMTFNTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKE 215

Query: 181 IVSMGGVSNPGDFIPMMNWVP-NGYKRKVSRLGKRMDRFLQGLIDEHRSNKEEERNTMID 240
           +VS+GG +N GDF+  + W   +G ++++ ++ KR D FLQGLIDEHR  K    NTMID
Sbjct: 216 VVSLGGANNVGDFLGFLRWFDFDGLEKRLKKISKRTDAFLQGLIDEHRFGKRNS-NTMID 275

Query: 241 HLLSLQETEPEYYGDEIIKGIVLVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDN 300
           HLL+ Q+++PEYY D+IIKG+++V+LLAGTDTS+VTIEWA+++LLN+PE++KKA+ ELD 
Sbjct: 276 HLLTQQQSQPEYYTDQIIKGLMVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDT 335

Query: 301 QIGEKQLAEELDVSKLPYLQGIIYETLRLNPAAPMLVPHLTSNNCTISEYEIPRDTIVLV 360
            IG  +  +E D+SKLPYLQ I+YETLRL+ AAP+LVPHL+S + ++  Y IP++TI++V
Sbjct: 336 HIGHDRQVDEHDISKLPYLQSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMV 395

Query: 361 NAWAIHRDPSQWEEPTLFKPERHQNLESSDHHQIPKMIPFGVGRRACPGSSMAQRVVGLT 420
           NAW IHRDP+ W +PT FKPER +        ++ K++ FG+GRRACPG +++QR  GLT
Sbjct: 396 NAWVIHRDPNLWSDPTCFKPERFEK-----EGEVNKLLSFGLGRRACPGENLSQRTEGLT 455

Query: 421 LATLIQCYEWERIGEEKVDMTEGRGVTMPKVVPLEAMCKAR 459
           L  LIQC+EW+RIGEEK+DM E +G+T  K   L AMCK R
Sbjct: 456 LGLLIQCFEWKRIGEEKIDMVEAKGITAGKKTSLNAMCKVR 490

[Close]

BLAST of Cucsa.102750.1 vs. Swiss-Prot
Match: C81E9_MEDTR (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 5.4e-155
Identity = 265/471 (56.26%), Postives = 354/471 (75.16%), Query Frame = 1


Query: 1   PPSLPIIGHLHYIKIPLHRTLHKLSAKYGPLISLRFGSRLVVVVSSLPAVEECFTKNDIV 60
           PP++PIIG+LH++K PLHRT   LS  YG + SL FGSRLVVVVSS     ECFTKNDI+
Sbjct: 34  PPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFSLWFGSRLVVVVSSPSLAHECFTKNDII 93

Query: 61  LANRPRLLFGKHIGYNHTTMVGSPYGDHWRNLRRIGAIEIFSASRLNKFAGTRKDEVERL 120
           LANRPR L GK+I YN+TT+  + YGDHWRNLRRI  I++ S +RLN F G R+DE  RL
Sbjct: 94  LANRPRFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNNRLNSFLGVRRDETNRL 153

Query: 121 LKKLSRNSMH---GYSKVEMQSSISELTFNISMRMAAGKRYFGDE--VRDVEEARQSREL 180
           ++KL ++ +    G++KVE++  ++E+TFN  MRM +GKRY+GD+  V DVEEA+Q RE+
Sbjct: 154 IQKLLKDVVSEGFGFTKVELRPRLTEMTFNAMMRMISGKRYYGDDGDVSDVEEAKQFREI 213

Query: 181 IKQIVSMGGVSNPGDFIPMMNWVP-NGYKRKVSRLGKRMDRFLQGLIDEHR-SNKEEERN 240
           I +++S+ G +N GDF+P++  V  +  +++  R+ KR + FL+GLI+EHR  N   +  
Sbjct: 214 ISEMMSLLGANNKGDFLPLLRVVDLDNLEKRCKRIAKRSNAFLEGLIEEHRRGNIHSDGG 273

Query: 241 TMIDHLLSLQETEPEYYGDEIIKGIVLVLLLAGTDTSAVTIEWALAHLLNNPEVLKKARE 300
           TMIDHLL L E++PEYY D +IKG++  +LLAGTDTSAVTIEW ++ LLN+PEVLKKA+E
Sbjct: 274 TMIDHLLKLSESQPEYYSDHLIKGLIQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKE 333

Query: 301 ELDNQIGEKQLAEELDVSKLPYLQGIIYETLRLNPAAPMLVPHLTSNNCTISEYEIPRDT 360
           ELD QIG+ +L +E D+SKLPYLQ II ETLRL+P AP+L+PH +S +CTI E+ +P+DT
Sbjct: 334 ELDTQIGKNKLVDEQDLSKLPYLQNIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDT 393

Query: 361 IVLVNAWAIHRDPSQWEEPTLFKPERHQNLESSDHHQIPKMIPFGVGRRACPGSSMAQRV 420
           I+L N W IHRDP  W +   FKPER +  E     ++ K++ FG+GRRACPG S+AQR 
Sbjct: 394 IILTNVWGIHRDPKHWNDALSFKPERFEKEE-----EVNKVMAFGLGRRACPGLSLAQRT 453

Query: 421 VGLTLATLIQCYEWERIGEEKVDMTEGRGVTMPKVVPLEAMCKARPIVHNI 465
           VG T+  LIQC+EWER  EEK+DM EG+G+TMP  +PL AMCKA PI +++
Sbjct: 454 VGFTVGLLIQCFEWERESEEKLDMMEGKGITMPMKIPLRAMCKALPIANDV 499

[Close]

BLAST of Cucsa.102750.1 vs. Swiss-Prot
Match: C8D11_ARATH (Cytochrome P450 81D11 OS=Arabidopsis thaliana GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 4.7e-151
Identity = 269/473 (56.87%), Postives = 342/473 (72.30%), Query Frame = 1


Query: 1   PPS----LPIIGHLHYIKIPLHRTLHKLSAKYG--PLISLRFGSRLVVVVSSLPAVEECF 60
           PPS     PIIGHLH +K+PLHR    LS       + SL  GSRLV VVSS    EECF
Sbjct: 40  PPSPSRPFPIIGHLHLLKLPLHRRFLSLSESLNNAKIFSLSLGSRLVFVVSSHAVAEECF 99

Query: 61  TKNDIVLANRPRLLFGKHIGYNHTTMVGSPYGDHWRNLRRIGAIEIFSASRLNKFAGTRK 120
           TKND+VLANRP  L GKHIGYN TTMVG+ YGD WRNLRRIG IEIFS+ RLN F   R+
Sbjct: 100 TKNDVVLANRPEFLVGKHIGYNSTTMVGAAYGDSWRNLRRIGTIEIFSSLRLNSFVSIRQ 159

Query: 121 DEVERLLKKLSRNSMHGYSKVEMQSSISELTFNISMRMAAGKRYFGDEVRDVEEARQSRE 180
           DE+ RL+  L++NS HG+ KVEM+     LT N  +RM AGKR++GD   +  EA+  R+
Sbjct: 160 DEIRRLIICLAKNSQHGFVKVEMKPLFMCLTINNIIRMVAGKRFYGDGTENDNEAKHVRQ 219

Query: 181 LIKQIVSMGGVSNPGDFIPMMNWVPNGYKRKVSRLGKRMDRFLQGLIDEHRSNKEEERNT 240
           LI ++V  GG  N  D+ P++ +V N Y++ V +L  R+D FLQ L++E R  K +  NT
Sbjct: 220 LIAEVVVSGGAGNAADYFPILRYVTN-YEKHVKKLAGRVDEFLQSLVNEKRVEKVKG-NT 279

Query: 241 MIDHLLSLQETEPEYYGDEIIKGIVLVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREE 300
           MIDHLLSLQET+P+YY D IIKGI+LV++LAGTDTSA T+EWA+++LLN+PEVL+KA+ E
Sbjct: 280 MIDHLLSLQETQPDYYTDVIIKGIILVMILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTE 339

Query: 301 LDNQIGEKQLAEELDVSKLPYLQGIIYETLRLNPAAPMLVPHLTSNNCTISEYEIPRDTI 360
           +D+QIG  +L EE D+ KLPYLQ I+ ETLRL P APML+PHL S +C +  Y++PR TI
Sbjct: 340 IDDQIGVDRLVEEQDIVKLPYLQHIVSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTI 399

Query: 361 VLVNAWAIHRDPSQWEEPTLFKPERHQNLESSDHHQIPKMIPFGVGRRACPGSSMAQRVV 420
           +LVNAWAIHRDP  WEEP  FKPER +        +  K++PFG+GRR+CPGS +AQR+V
Sbjct: 400 ILVNAWAIHRDPKLWEEPEKFKPERFEK-----KGEDKKLMPFGIGRRSCPGSGLAQRLV 459

Query: 421 GLTLATLIQCYEWERIGEEKVDMTEG-RGVTMPKVVPLEAMCKARPIVHNIFN 467
            L L +L+QC+EWER+ E+ +DM E  +G TM K   L+AMCKARPIVH + +
Sbjct: 460 TLALGSLVQCFEWERVEEKYLDMRESEKGTTMRKATSLQAMCKARPIVHKVLD 505

[Close]

BLAST of Cucsa.102750.1 vs. Swiss-Prot
Match: C81E7_MEDTR (Isoflavone 2'-hydroxylase OS=Medicago truncatula GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 3.7e-148
Identity = 248/468 (52.99%), Postives = 352/468 (75.21%), Query Frame = 1


Query: 1   PPSLPIIGHLHYIKIPLHRTLHKLSAKYGPLISLRFGSRLVVVVSSLPAVEECFTKNDIV 60
           P SLPIIG+LH++K PL+RT   L+ KYG +ISL FGSRLVVVVSSL   +ECFTKND+V
Sbjct: 36  PTSLPIIGNLHHLKRPLNRTFKALTEKYGNVISLWFGSRLVVVVSSLSEFQECFTKNDVV 95

Query: 61  LANRPRLLFGKHIGYNHTTMVGSPYGDHWRNLRRIGAIEIFSASRLNKFAGTRKDEVERL 120
           LANRPR L GK+I YN+TT+  + YG+HWRNLRRI ++++ S  R+N FA  R+DE +RL
Sbjct: 96  LANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITSLDVLSNHRINNFAPIRRDETQRL 155

Query: 121 LKKLSRNSMHGYSKVEMQSSISELTFNISMRMAAGKRYFGDE--VRDVEEARQSRELIKQ 180
           +KKL+ +S   +++VE+     ++TFN  MRM +GKRY+GD+  + +V+EA Q R+++ +
Sbjct: 156 IKKLAEDSSTKFAEVELTFRFFDMTFNNIMRMISGKRYYGDDCDISEVQEASQFRDMVSE 215

Query: 181 IVSMGGVSNPGDFIPMMNWVP-NGYKRKVSRLGKRMDRFLQGLIDEHRSNKEEERNTMID 240
           ++ + G +N  DF+P++ ++     +++V R+G++ D FL GL+ E RS K+E  NTMID
Sbjct: 216 LLQLSGANNKTDFMPLLKFLDFENLEKRVKRIGEKNDVFLSGLLQEQRS-KKERTNTMID 275

Query: 241 HLLSLQETEPEYYGDEIIKGIVLVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDN 300
           HLL++QE++PEYY D IIKG+ L +LLAGTD+SAVT+EW ++++LN PEVLKK R+E+D 
Sbjct: 276 HLLNMQESQPEYYTDTIIKGLCLAMLLAGTDSSAVTLEWTMSNILNYPEVLKKVRDEVDT 335

Query: 301 QIGEKQLAEELDVSKLPYLQGIIYETLRLNPAAPMLVPHLTSNNCTISEYEIPRDTIVLV 360
            +G+ +L +E D+ KL YL+ +IYETLRL   AP+L+PH T++ C +  Y++PRDTIVL+
Sbjct: 336 HVGQDRLVDESDLPKLTYLRNVIYETLRLYTPAPLLLPHSTADECIMGGYKVPRDTIVLI 395

Query: 361 NAWAIHRDPSQWEEPTLFKPERHQNLESSDHHQIPKMIPFGVGRRACPGSSMAQRVVGLT 420
           NAWAIHRDP  W E T FKPER          ++ KMI FG+GRRACPG  +A R + +T
Sbjct: 396 NAWAIHRDPETWSEATTFKPERFDK-----KGELEKMIAFGMGRRACPGEGLALRAISMT 455

Query: 421 LATLIQCYEWERIGEEKVDMTEGRGVTMPKVVPLEAMCKARPIVHNIF 466
           LA L+QC++W+RI +EK+DM+E  G TM K++PL+AMCK RP+V+ +F
Sbjct: 456 LALLVQCFDWKRINDEKIDMSERDGFTMTKLLPLKAMCKTRPVVNKVF 497

[Close]

BLAST of Cucsa.102750.1 vs. Swiss-Prot
Match: C81D1_ARATH (Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 2.0e-146
Identity = 256/476 (53.78%), Postives = 350/476 (73.53%), Query Frame = 1


Query: 1   PPS----LPIIGHLHYIKIPLHRTLHKLSAKY-----GPLISLRFGSRLVVVVSSLP-AV 60
           PPS    LPIIGHL  +K P+HRTL   S        G ++SLR GSRLV VVSS   A 
Sbjct: 34  PPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDHNDGGGVMSLRLGSRLVYVVSSHKVAA 93

Query: 61  EECFTKNDIVLANRPRLLFGKHIGYNHTTMVGSPYGDHWRNLRRIGAIEIFSASRLNKFA 120
           EECF KND+VLANRP+++ GKH+GYN+T M+ +PYGDHWRNLRR+  IEIFS  RLN F 
Sbjct: 94  EECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPYGDHWRNLRRLCTIEIFSTHRLNCFL 153

Query: 121 GTRKDEVERLLKKLSRNSMHGYSKVEMQSSISELTFNISMRMAAGKRYFGDEVRDVEEAR 180
             R DEV RL+ +LSR +    + VE++  + +LTFN  MRM  GKRY+G+E  D EEA+
Sbjct: 154 YVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDLTFNNIMRMMTGKRYYGEETTDEEEAK 213

Query: 181 QSRELIKQIVSMGGVSNPGDFIPMMNWVPNGYKRKVSRLGKRMDRFLQGLIDEHRSNKEE 240
           + R+L+  + +     N  D++P++    + Y+ +V +LG+  D+FLQGLID+ R  ++E
Sbjct: 214 RVRKLVADVGANTSSGNAVDYVPILRLF-SSYENRVKKLGEETDKFLQGLIDDKRG-QQE 273

Query: 241 ERNTMIDHLLSLQETEPEYYGDEIIKGIVLVLLLAGTDTSAVTIEWALAHLLNNPEVLKK 300
              TMIDHLL LQ+++ EYY D+IIKGI+L++++AGT+TSAVT+EWAL++LLN+P+V+ K
Sbjct: 274 TGTTMIDHLLVLQKSDIEYYTDQIIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISK 333

Query: 301 AREELDNQIGEKQLAEELDVSKLPYLQGIIYETLRLNPAAPMLVPHLTSNNCTISEYEIP 360
           AR+E+DN++G  +L EE D+S+LPYL+ I+ ETLRL+PA P+LVPH+ S +C I  Y++P
Sbjct: 334 ARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMP 393

Query: 361 RDTIVLVNAWAIHRDPSQWEEPTLFKPERHQNLESSDHHQIPKMIPFGVGRRACPGSSMA 420
           R T +LVNAWAIHRDP+ W++P  FKPER +  E +      K++ FG+GRRACPGS +A
Sbjct: 394 RGTTLLVNAWAIHRDPNTWDDPDSFKPERFEKEEEAQ-----KLLAFGLGRRACPGSGLA 453

Query: 421 QRVVGLTLATLIQCYEWERIGEEKVDMTEGRGVTMPKVVPLEAMCKARPIVHNIFN 467
           QR+VGL L +LIQC+EWER+G  +VDM EG G T+PK +PL+A+CKARP +H I +
Sbjct: 454 QRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTVPKAIPLKAICKARPFLHKIIS 502

[Close]

BLAST of Cucsa.102750.1 vs. TrEMBL
Match: A0A0A0KLF2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G492220 PE=3 SV=1)

HSP 1 Score: 940.3 bits (2429), Expect = 9.2e-271
Identity = 466/466 (100.00%), Postives = 466/466 (100.00%), Query Frame = 1


Query: 1   PPSLPIIGHLHYIKIPLHRTLHKLSAKYGPLISLRFGSRLVVVVSSLPAVEECFTKNDIV 60
           PPSLPIIGHLHYIKIPLHRTLHKLSAKYGPLISLRFGSRLVVVVSSLPAVEECFTKNDIV
Sbjct: 33  PPSLPIIGHLHYIKIPLHRTLHKLSAKYGPLISLRFGSRLVVVVSSLPAVEECFTKNDIV 92

Query: 61  LANRPRLLFGKHIGYNHTTMVGSPYGDHWRNLRRIGAIEIFSASRLNKFAGTRKDEVERL 120
           LANRPRLLFGKHIGYNHTTMVGSPYGDHWRNLRRIGAIEIFSASRLNKFAGTRKDEVERL
Sbjct: 93  LANRPRLLFGKHIGYNHTTMVGSPYGDHWRNLRRIGAIEIFSASRLNKFAGTRKDEVERL 152

Query: 121 LKKLSRNSMHGYSKVEMQSSISELTFNISMRMAAGKRYFGDEVRDVEEARQSRELIKQIV 180
           LKKLSRNSMHGYSKVEMQSSISELTFNISMRMAAGKRYFGDEVRDVEEARQSRELIKQIV
Sbjct: 153 LKKLSRNSMHGYSKVEMQSSISELTFNISMRMAAGKRYFGDEVRDVEEARQSRELIKQIV 212

Query: 181 SMGGVSNPGDFIPMMNWVPNGYKRKVSRLGKRMDRFLQGLIDEHRSNKEEERNTMIDHLL 240
           SMGGVSNPGDFIPMMNWVPNGYKRKVSRLGKRMDRFLQGLIDEHRSNKEEERNTMIDHLL
Sbjct: 213 SMGGVSNPGDFIPMMNWVPNGYKRKVSRLGKRMDRFLQGLIDEHRSNKEEERNTMIDHLL 272

Query: 241 SLQETEPEYYGDEIIKGIVLVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDNQIG 300
           SLQETEPEYYGDEIIKGIVLVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDNQIG
Sbjct: 273 SLQETEPEYYGDEIIKGIVLVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDNQIG 332

Query: 301 EKQLAEELDVSKLPYLQGIIYETLRLNPAAPMLVPHLTSNNCTISEYEIPRDTIVLVNAW 360
           EKQLAEELDVSKLPYLQGIIYETLRLNPAAPMLVPHLTSNNCTISEYEIPRDTIVLVNAW
Sbjct: 333 EKQLAEELDVSKLPYLQGIIYETLRLNPAAPMLVPHLTSNNCTISEYEIPRDTIVLVNAW 392

Query: 361 AIHRDPSQWEEPTLFKPERHQNLESSDHHQIPKMIPFGVGRRACPGSSMAQRVVGLTLAT 420
           AIHRDPSQWEEPTLFKPERHQNLESSDHHQIPKMIPFGVGRRACPGSSMAQRVVGLTLAT
Sbjct: 393 AIHRDPSQWEEPTLFKPERHQNLESSDHHQIPKMIPFGVGRRACPGSSMAQRVVGLTLAT 452

Query: 421 LIQCYEWERIGEEKVDMTEGRGVTMPKVVPLEAMCKARPIVHNIFN 467
           LIQCYEWERIGEEKVDMTEGRGVTMPKVVPLEAMCKARPIVHNIFN
Sbjct: 453 LIQCYEWERIGEEKVDMTEGRGVTMPKVVPLEAMCKARPIVHNIFN 498

[Close]

BLAST of Cucsa.102750.1 vs. TrEMBL
Match: A0A0A0KJM0_CUCSA (Cytochrome P450 OS=Cucumis sativus GN=Csa_6G492230 PE=3 SV=1)

HSP 1 Score: 864.0 bits (2231), Expect = 8.4e-248
Identity = 427/466 (91.63%), Postives = 445/466 (95.49%), Query Frame = 1


Query: 1   PPSLPIIGHLHYIKIPLHRTLHKLSAKYGPLISLRFGSRLVVVVSSLPAVEECFTKNDIV 60
           PPSLPIIGHLHYIKIP HRTLHKLSAKYGP+ISL FGSRLVVVVSSLPAVEECFTKNDIV
Sbjct: 33  PPSLPIIGHLHYIKIPRHRTLHKLSAKYGPVISLWFGSRLVVVVSSLPAVEECFTKNDIV 92

Query: 61  LANRPRLLFGKHIGYNHTTMVGSPYGDHWRNLRRIGAIEIFSASRLNKFAGTRKDEVERL 120
           LANRPRLL GKH+GYNHTTMV SPYGDHWRNLRRIGAIEIFSASRLNKFAGTRKDEVERL
Sbjct: 93  LANRPRLLVGKHVGYNHTTMVASPYGDHWRNLRRIGAIEIFSASRLNKFAGTRKDEVERL 152

Query: 121 LKKLSRNSMHGYSKVEMQSSISELTFNISMRMAAGKRYFGDEVRDVEEARQSRELIKQIV 180
           LKKLSRNSM+G+SKVEMQSSISELTFNISMRMAAGKRYFG+EVRDVEEARQSRELIKQIV
Sbjct: 153 LKKLSRNSMYGFSKVEMQSSISELTFNISMRMAAGKRYFGEEVRDVEEARQSRELIKQIV 212

Query: 181 SMGGVSNPGDFIPMMNWVPNGYKRKVSRLGKRMDRFLQGLIDEHRSNKEEERNTMIDHLL 240
           SMGGVSNPGDFIPMMNW+PNG+KRKV R+ KRMD FLQGLIDEHRSNKEEERNTMIDHLL
Sbjct: 213 SMGGVSNPGDFIPMMNWIPNGFKRKVWRVAKRMDGFLQGLIDEHRSNKEEERNTMIDHLL 272

Query: 241 SLQETEPEYYGDEIIKGIVLVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDNQIG 300
           SLQETEPEYYGD+IIKGI+LVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELD QIG
Sbjct: 273 SLQETEPEYYGDQIIKGIILVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDTQIG 332

Query: 301 EKQLAEELDVSKLPYLQGIIYETLRLNPAAPMLVPHLTSNNCTISEYEIPRDTIVLVNAW 360
           EK+L EE DVSKLPYLQGII ETLRLNPAAPMLVPHLTSN+CTIS Y+IPRDTIVLVNAW
Sbjct: 333 EKRLVEESDVSKLPYLQGIISETLRLNPAAPMLVPHLTSNDCTISGYKIPRDTIVLVNAW 392

Query: 361 AIHRDPSQWEEPTLFKPERHQNLESSDHHQIPKMIPFGVGRRACPGSSMAQRVVGLTLAT 420
           AIHRDP QWEEPTLFKPERHQ  ES DHH I K+IPFGVGRRACPGS MAQRVVGLTLA 
Sbjct: 393 AIHRDPHQWEEPTLFKPERHQKSESIDHH-ISKLIPFGVGRRACPGSGMAQRVVGLTLAA 452

Query: 421 LIQCYEWERIGEEKVDMTEGRGVTMPKVVPLEAMCKARPIVHNIFN 467
           LIQCYEWERIG+EKVDM+EGRGVTMPK+VPLEAMCK RPI+HNIFN
Sbjct: 453 LIQCYEWERIGDEKVDMSEGRGVTMPKMVPLEAMCKPRPIIHNIFN 497

[Close]

BLAST of Cucsa.102750.1 vs. TrEMBL
Match: A0A0A0KFV2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G492250 PE=3 SV=1)

HSP 1 Score: 684.5 bits (1765), Expect = 9.1e-194
Identity = 320/465 (68.82%), Postives = 403/465 (86.67%), Query Frame = 1


Query: 1   PPSLPIIGHLHYIKIPLHRTLHKLSAKYGPLISLRFGSRLVVVVSSLPAVEECFTKNDIV 60
           PPS P+IGHLH +K P+HRT   LSAKYGP++SLR GS+L VV+SS  AVE+CFTKND+V
Sbjct: 38  PPSFPVIGHLHLLKKPIHRTFSNLSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVV 97

Query: 61  LANRPRLLFGKHIGYNHTTMVGSPYGDHWRNLRRIGAIEIFSASRLNKFAGTRKDEVERL 120
           LANRPRLL GK+IGYN+TTM+G+PYGDHWRNLRRIGAIEIFS SR+NKF   R+DEV R+
Sbjct: 98  LANRPRLLVGKYIGYNYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRI 157

Query: 121 LKKLSRNSMHGYSKVEMQSSISELTFNISMRMAAGKRYFGDEVRDVEEARQSRELIKQIV 180
           ++KLS NS+H +SKVE+QS++SELTFNI+MRMAAGKRY+G++V + EEAR+ RELIK+IV
Sbjct: 158 VRKLSHNSIHQFSKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIV 217

Query: 181 SMGGVSNPGDFIPMMNWVPNGYKRKVSRLGKRMDRFLQGLIDEHRSNKEEERNTMIDHLL 240
           ++GGVSNPGDF+P++NW+  G +RK+ +LGK++D FLQGLID+HR  KEE RNTMIDHLL
Sbjct: 218 AVGGVSNPGDFLPVLNWMSKGIERKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLL 277

Query: 241 SLQETEPEYYGDEIIKGIVLVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDNQIG 300
           SLQ++EP++Y D+IIKG +L+LL AG+DTSAVT+EWAL+HLLNNP+VLKKA+EE+D QIG
Sbjct: 278 SLQQSEPDHYNDQIIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIG 337

Query: 301 EKQLAEELDVSKLPYLQGIIYETLRLNPAAPMLVPHLTSNNCTISEYEIPRDTIVLVNAW 360
           +++L EE D+SKLPYLQ I+ ETLRLNPAAPMLVPH  +N+C I  Y++PRDT+VLVNAW
Sbjct: 338 QERLVEESDISKLPYLQRIVSETLRLNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAW 397

Query: 361 AIHRDPSQWEEPTLFKPERHQNLESSDHHQIPKMIPFGVGRRACPGSSMAQRVVGLTLAT 420
           AIHRD ++WE+   FKPER++  E+ + H   K +PFG+GRRACPGS+MA RV+GLTLAT
Sbjct: 398 AIHRDSNEWEDCMSFKPERYEKSEAVEIH---KFLPFGLGRRACPGSAMAHRVMGLTLAT 457

Query: 421 LIQCYEWERIGEEKVDMTEGRGVTMPKVVPLEAMCKARPIVHNIF 466
           LIQC+EW++IGEE +DM E  G  M K+VPLEAMCKARPI++NIF
Sbjct: 458 LIQCFEWKKIGEEDIDMKELSGAIMTKMVPLEAMCKARPIINNIF 499

[Close]

BLAST of Cucsa.102750.1 vs. TrEMBL
Match: A0A061EFX4_THECC (Cytochrome P450, family 81, subfamily D, polypeptide 8, putative OS=Theobroma cacao GN=TCM_019137 PE=3 SV=1)

HSP 1 Score: 631.7 bits (1628), Expect = 7.0e-178
Identity = 311/470 (66.17%), Postives = 385/470 (81.91%), Query Frame = 1


Query: 1   PPSLPIIGHLHYIKIPLHRTLHKLSAKYGPLISLRFGSRLVVVVSSLPAVEECFTKNDIV 60
           PPSLP +GHLH IK P+HR  H LS KYGP+ SLRFGSRLVVVVSS  A  ECFTKNDIV
Sbjct: 38  PPSLPFLGHLHLIKRPIHRFYHSLSQKYGPIFSLRFGSRLVVVVSSPTAAGECFTKNDIV 97

Query: 61  LANRPRLLFGKHIGYNHTTMVGSPYGDHWRNLRRIGAIEIFSASRLNKFAGTRKDEVERL 120
           LANRP+LL GK++GYN +T+VGSPYGDHWRNLRRI AIEIFS+SRLN F   RKDEV+RL
Sbjct: 98  LANRPKLLIGKYLGYNWSTVVGSPYGDHWRNLRRISAIEIFSSSRLNAFLSIRKDEVKRL 157

Query: 121 LKKLSR--NSMHGYSKVEMQSSISELTFNISMRMAAGKRYFG-DEVRDVEEARQSRELIK 180
           L KLS   NS   ++KVE++S   +LTFN  MRM AGKRY+G D V D  EA++ REL+K
Sbjct: 158 LLKLSSDDNSGQEFTKVELRSLFVDLTFNNIMRMVAGKRYYGGDSVTDEGEAKEFRELMK 217

Query: 181 QIVSMGGVSNPGDFIPMMNWVPN-GYKRKVSRLGKRMDRFLQGLIDEHRSNKEEERNTMI 240
           + ++ GGV++PGDF+P++NW+    Y +K+  LG+RMDRFLQ LIDE R+ ++   NTMI
Sbjct: 218 ESLASGGVAHPGDFLPILNWIGGRSYLKKLMNLGERMDRFLQKLIDEVRAKRQG--NTMI 277

Query: 241 DHLLSLQETEPEYYGDEIIKGIVLVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELD 300
           DHLLSLQ+TEP+YY D++IKG++LV+LLAGTDTSAVT+EWA+++LLNNP+VLKKAR ELD
Sbjct: 278 DHLLSLQQTEPDYYTDQLIKGLILVMLLAGTDTSAVTLEWAMSNLLNNPDVLKKARTELD 337

Query: 301 NQIGEKQLAEELDVSKLPYLQGIIYETLRLNPAAPMLVPHLTSNNCTISEYEIPRDTIVL 360
           +QIG++ L +E DVSKL YLQ II+ETLRLNPAAP+L+PH+ S +C I EY++PRDTIVL
Sbjct: 338 SQIGQENLIDEPDVSKLQYLQSIIFETLRLNPAAPLLLPHMASTDCKICEYDVPRDTIVL 397

Query: 361 VNAWAIHRDPSQWEEPTLFKPERHQNLESSDHHQIPKMIPFGVGRRACPGSSMAQRVVGL 420
           +NAWAIHRD + WE+PT FKPER+ N E   +    K++PFG+GRRACPG+ +AQRVVGL
Sbjct: 398 INAWAIHRDSTLWEDPTSFKPERYGNGERESN----KLMPFGLGRRACPGAGLAQRVVGL 457

Query: 421 TLATLIQCYEWERIGEEKVDMTEGRGVTMPKVVPLEAMCKARPIVHNIFN 467
           TL +LIQC+EWER+ E++VDM EG G TMPKVV LEAMCKARPIV+ + N
Sbjct: 458 TLGSLIQCFEWERVSEKEVDMAEGDGTTMPKVVALEAMCKARPIVNKVLN 501

[Close]

BLAST of Cucsa.102750.1 vs. TrEMBL
Match: W9QYM5_9ROSA (Isoflavone 2'-hydroxylase OS=Morus notabilis GN=L484_009304 PE=3 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 2.7e-177
Identity = 305/462 (66.02%), Postives = 377/462 (81.60%), Query Frame = 1


Query: 1   PPSLPIIGHLHYIKIPLHRTLHKLSAKYGPLISLRFGSRLVVVVSSLPAVEECFTKNDIV 60
           PPSLPIIGHLH +KIP+HR L++LS KYGP+ SL FGSR VVVVSS  AV ECFTKND+V
Sbjct: 37  PPSLPIIGHLHLLKIPIHRALYRLSEKYGPVFSLWFGSRRVVVVSSPSAVTECFTKNDVV 96

Query: 61  LANRPRLLFGKHIGYNHTTMVGSPYGDHWRNLRRIGAIEIFSASRLNKFAGTRKDEVERL 120
           LANRP LL  KH+GYN+TTM  SPYGDHWRNLR IGAI IFSASRL  F G R+DE++RL
Sbjct: 97  LANRPPLLMSKHLGYNYTTMASSPYGDHWRNLRSIGAINIFSASRLTTFLGIRRDEIKRL 156

Query: 121 LKKLSRNSMHGYSKVEMQSSISELTFNISMRMAAGKRYFGDEVRDVEEARQSRELIKQIV 180
           L KLSRNSM G+++VE++S  SE+TFNI MR  AGKRY+GD+V D +EA + R+++K+  
Sbjct: 157 LVKLSRNSMDGFARVELKSMFSEMTFNIIMRTVAGKRYYGDDVTDEDEAARFRKIMKEAS 216

Query: 181 SMGGVSNPGDFIPMMN-WVPNGYKRKVSRLGKRMDRFLQGLIDEHRSNKEEERNTMIDHL 240
           + GG  NPGDF+P++N +V  GY+ KV R+ K  D FLQGLIDEHR  K+E RNTMI HL
Sbjct: 217 AYGGAVNPGDFLPILNLFVKEGYEEKVKRIAKAADSFLQGLIDEHRI-KKESRNTMIGHL 276

Query: 241 LSLQETEPEYYGDEIIKGIVLVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDNQI 300
           LSL+E++PEYY D+IIKG+++ LLLAGTDTS+VT+EWA+++LLN+P VLKKAR ELD Q+
Sbjct: 277 LSLEESQPEYYTDQIIKGLIMNLLLAGTDTSSVTLEWAMSNLLNHPHVLKKARAELDAQV 336

Query: 301 GEKQLAEELDVSKLPYLQGIIYETLRLNPAAPMLVPHLTSNNCTISEYEIPRDTIVLVNA 360
           G++Q+ +E D+SKL +LQ II ETLRL PAAP+LVPH +S++CTI  Y++PRDTI+LVNA
Sbjct: 337 GQRQMVDEPDLSKLSHLQNIISETLRLYPAAPLLVPHYSSDDCTIDGYDVPRDTILLVNA 396

Query: 361 WAIHRDPSQWEEPTLFKPERHQNLESSDHHQIPKMIPFGVGRRACPGSSMAQRVVGLTLA 420
           WAIHRDP  W++   FKPER +   S    Q  KM+PFG+GRRACPG+ MAQRVVGLTLA
Sbjct: 397 WAIHRDPKLWDDAESFKPERFET-SSEGGDQDTKMMPFGLGRRACPGAGMAQRVVGLTLA 456

Query: 421 TLIQCYEWERIGEEKVDMTEGRGVTMPKVVPLEAMCKARPIV 462
           +LIQC+EWER+ EE++DM EGRGV MPK VPLE MCKAR I+
Sbjct: 457 SLIQCFEWERVSEEEIDMVEGRGVIMPKAVPLEVMCKARTIM 496

[Close]

BLAST of Cucsa.102750.1 vs. TAIR10
Match: AT4G37340.1 (AT4G37340.1 cytochrome P450, family 81, subfamily D, polypeptide 3)

HSP 1 Score: 546.6 bits (1407), Expect = 1.5e-155
Identity = 270/473 (57.08%), Postives = 347/473 (73.36%), Query Frame = 1


Query: 1   PPS----LPIIGHLHYIKIPLHRTLHKLSAKYG--PLISLRFGSRLVVVVSSLPAVEECF 60
           PPS    LP+IGHL  +K PLHR    +S   G  P+ISLR G+RLV VVSS    EECF
Sbjct: 31  PPSPSWALPVIGHLRLLKPPLHRVFLSVSESLGDAPIISLRLGNRLVFVVSSHSLAEECF 90

Query: 61  TKNDIVLANRPRLLFGKHIGYNHTTMVGSPYGDHWRNLRRIGAIEIFSASRLNKFAGTRK 120
           TKND+VLANR   L  KHI Y  TT+V + YGDHWRNLRRIGA+EIFSA RLN F+  R+
Sbjct: 91  TKNDVVLANRFNSLASKHISYGCTTVVTASYGDHWRNLRRIGAVEIFSAHRLNSFSSIRR 150

Query: 121 DEVERLLKKLSRNSMHGYSKVEMQSSISELTFNISMRMAAGKRYFGDEVRDVEEARQSRE 180
           DE+ RL+  LSRNS   ++KVEM+S  S LTFN  +RM AGK Y+GD   D  EA++ RE
Sbjct: 151 DEIHRLIACLSRNSSLEFTKVEMKSMFSNLTFNNIIRMLAGKCYYGDGAEDDPEAKRVRE 210

Query: 181 LIKQIVSMGGVSNPGDFIPMMNWVPNGYKRKVSRLGKRMDRFLQGLIDEHRSNKEEERNT 240
           LI + +   G  N  D++P++ W+  G ++++ ++  R+D FLQGL+DE R  KE+ +NT
Sbjct: 211 LIAEGMGCFGAGNTADYLPILTWI-TGSEKRIKKIASRLDEFLQGLVDERREGKEKRQNT 270

Query: 241 MIDHLLSLQETEPEYYGDEIIKGIVLVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREE 300
           M+DHLL LQET+PEYY D IIKGI+L L+LAGTDTSAVT+EW L+ LLN+P++L KAR+E
Sbjct: 271 MVDHLLCLQETQPEYYTDNIIKGIMLSLILAGTDTSAVTLEWTLSALLNHPQILSKARDE 330

Query: 301 LDNQIGEKQLAEELDVSKLPYLQGIIYETLRLNPAAPMLVPHLTSNNCTISEYEIPRDTI 360
           +DN++G  +L EE D+S LPYLQ I+ E+LRL PA+P+LVPH+ S +C +  Y +PR T+
Sbjct: 331 IDNKVGLNRLVEESDLSHLPYLQNIVSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTM 390

Query: 361 VLVNAWAIHRDPSQWEEPTLFKPERHQNLESSDHHQIPKMIPFGVGRRACPGSSMAQRVV 420
           +L NAWAIHRDP  W++PT FKPER +        +  K++ FG+GRRACPGS +AQR+ 
Sbjct: 391 LLTNAWAIHRDPKIWDDPTSFKPERFEK-----EGEAQKLLGFGLGRRACPGSGLAQRLA 450

Query: 421 GLTLATLIQCYEWERIGEEKVDMTE-GRGVTMPKVVPLEAMCKARPIVHNIFN 467
            LT+ +LIQC+EWERIGEE+VDMTE G GV MPK +PL AMCKARP+V  I N
Sbjct: 451 SLTIGSLIQCFEWERIGEEEVDMTEGGGGVIMPKAIPLVAMCKARPVVGKILN 497

[Close]

BLAST of Cucsa.102750.1 vs. TAIR10
Match: AT4G37360.1 (AT4G37360.1 cytochrome P450, family 81, subfamily D, polypeptide 2)

HSP 1 Score: 542.3 bits (1396), Expect = 2.8e-154
Identity = 267/470 (56.81%), Postives = 343/470 (72.98%), Query Frame = 1


Query: 1   PPS----LPIIGHLHYIKIPLHRTLHKLSAKYG--PLISLRFGSRLVVVVSSLPAVEECF 60
           PPS    LP+IGHL  +K PLHR    +S   G  P+ISLR G+RL+ VVSS    EECF
Sbjct: 31  PPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLGDAPIISLRLGNRLLFVVSSHSIAEECF 90

Query: 61  TKNDIVLANRPRLLFGKHIGYNHTTMVGSPYGDHWRNLRRIGAIEIFSASRLNKFAGTRK 120
           TKND++LANR   +  KHI Y ++T+V + Y +HWRNLRRIGA+EIFSA RLN F+  R+
Sbjct: 91  TKNDVILANRQTTISTKHISYGNSTVVSASYSEHWRNLRRIGALEIFSAHRLNSFSSIRR 150

Query: 121 DEVERLLKKLSRNSMHGYSKVEMQSSISELTFNISMRMAAGKRYFGDEVRDVEEARQSRE 180
           DE+ RL+ +L RNS +G++KVEM+S  S+LTFN  +RM AGK Y+GD   D  EA++ R 
Sbjct: 151 DEIRRLIGRLLRNSSYGFTKVEMKSMFSDLTFNNIIRMLAGKCYYGDGKEDDPEAKRVRT 210

Query: 181 LIKQIVSMGGVSNPGDFIPMMNWVPNGYKRKVSRLGKRMDRFLQGLIDEHRSNKEEERNT 240
           LI + +S  G  N  D+IP++ W+     R + +L  R+D FLQGL+DE R  KE++ NT
Sbjct: 211 LIAEAMSSSGPGNAADYIPILTWITYSETR-IKKLAGRLDEFLQGLVDEKREGKEKKENT 270

Query: 241 MIDHLLSLQETEPEYYGDEIIKGIVLVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREE 300
           M+DHLL LQET+PEYY D IIKG +L L+  GTDT+AVT+EWAL+ LLNNPEVL KAR+E
Sbjct: 271 MVDHLLCLQETQPEYYMDRIIKGTMLSLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDE 330

Query: 301 LDNQIGEKQLAEELDVSKLPYLQGIIYETLRLNPAAPMLVPHLTSNNCTISEYEIPRDTI 360
           +D  IG  +L EE D+  LPYLQ I+ ETLRL PAAPML+PH+ S +C +  Y++PR T+
Sbjct: 331 IDRMIGVDRLLEESDIPNLPYLQNIVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTM 390

Query: 361 VLVNAWAIHRDPSQWEEPTLFKPERHQNLESSDHHQIPKMIPFGVGRRACPGSSMAQRVV 420
           +L NAWAIHRDP  W++PT FKPER +        +  K++PFG+GRRACPGS +AQR+V
Sbjct: 391 LLTNAWAIHRDPLLWDDPTSFKPERFEK-----EGEAKKLMPFGLGRRACPGSGLAQRLV 450

Query: 421 GLTLATLIQCYEWERIGEEKVDMTEGRGVTMPKVVPLEAMCKARPIVHNI 465
            L+L +LIQC+EWERIGEE+VDMTEG G+TMPK  PLEAMC+AR  V  I
Sbjct: 451 TLSLGSLIQCFEWERIGEEEVDMTEGPGLTMPKARPLEAMCRARDFVGKI 494

[Close]

BLAST of Cucsa.102750.1 vs. TAIR10
Match: AT4G37370.1 (AT4G37370.1 cytochrome P450, family 81, subfamily D, polypeptide 8)

HSP 1 Score: 541.6 bits (1394), Expect = 4.8e-154
Identity = 265/466 (56.87%), Postives = 348/466 (74.68%), Query Frame = 1


Query: 3   SLPIIGHLHYIKIPLHRTLHKLSAKYG--PLISLRFGSRLVVVVSSLPAVEECFTKNDIV 62
           SLP+IGHL  +K P+HRT   LS      P+ SLR G+RLV V SS    EECFTKND+V
Sbjct: 37  SLPVIGHLRLLKPPIHRTFLSLSQSLNNAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVV 96

Query: 63  LANRPRLLFGKHIGYNHTTMVGSPYGDHWRNLRRIGAIEIFSASRLNKFAGTRKDEVERL 122
           LANRP  +  KH+ Y++TTM+ + YGDHWRNLRRIG++EIFS  RLN F   RKDE+ RL
Sbjct: 97  LANRPNFILAKHVAYDYTTMIAASYGDHWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRL 156

Query: 123 LKKLSRNSMHGYSKVEMQSSISELTFNISMRMAAGKRYFGDEVRDVEEARQSRELIKQIV 182
           + +LSRN    + KV+M+S +S+LTFN  +RM AGKRY+GD V D  EA++ R+LI  +V
Sbjct: 157 VFRLSRNFSQEFVKVDMKSMLSDLTFNNILRMVAGKRYYGDGVEDDPEAKRVRQLIADVV 216

Query: 183 SMGGVSNPGDFIPMMNWVPNGYKRKVSRLGKRMDRFLQGLIDEHRSNKEEERNTMIDHLL 242
           +  G  N  D++P++  V + Y+ +V +L  R+D FLQGL+DE R  KE+  NTMIDHLL
Sbjct: 217 ACAGAGNAVDYLPVLRLVSD-YETRVKKLAGRLDEFLQGLVDEKREAKEKG-NTMIDHLL 276

Query: 243 SLQETEPEYYGDEIIKGIVLVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDNQIG 302
           +LQE++P+Y+ D IIKG +L L+LAGTDTSAVT+EWAL+++LN+P+VL KAR+E+D +IG
Sbjct: 277 TLQESQPDYFTDRIIKGNMLALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIG 336

Query: 303 EKQLAEELDVSKLPYLQGIIYETLRLNPAAPMLVPHLTSNNCTISEYEIPRDTIVLVNAW 362
             +L +E D+S LPYLQ I+ ETLRL PAAPML+PH+ S +C ++ Y++PR TI+L N W
Sbjct: 337 LDRLMDESDISNLPYLQNIVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVW 396

Query: 363 AIHRDPSQWEEPTLFKPERHQNLESSDHHQIPKMIPFGVGRRACPGSSMAQRVVGLTLAT 422
           AIHRDP  W++P  FKPER +        +  K++PFG+GRRACPGS +A R++ LTL +
Sbjct: 397 AIHRDPQLWDDPMSFKPERFEK-----EGEAQKLMPFGLGRRACPGSGLAHRLINLTLGS 456

Query: 423 LIQCYEWERIGEEKVDMTEGRGVTMPKVVPLEAMCKARPIVHNIFN 467
           LIQC EWE+IGEE VDM+EG+GVTMPK  PLEAMC+ARP V  IFN
Sbjct: 457 LIQCLEWEKIGEE-VDMSEGKGVTMPKAKPLEAMCRARPSVVKIFN 494

[Close]

BLAST of Cucsa.102750.1 vs. TAIR10
Match: AT3G28740.1 (AT3G28740.1 Cytochrome P450 superfamily protein)

HSP 1 Score: 535.8 bits (1379), Expect = 2.7e-152
Identity = 269/473 (56.87%), Postives = 342/473 (72.30%), Query Frame = 1


Query: 1   PPS----LPIIGHLHYIKIPLHRTLHKLSAKYG--PLISLRFGSRLVVVVSSLPAVEECF 60
           PPS     PIIGHLH +K+PLHR    LS       + SL  GSRLV VVSS    EECF
Sbjct: 40  PPSPSRPFPIIGHLHLLKLPLHRRFLSLSESLNNAKIFSLSLGSRLVFVVSSHAVAEECF 99

Query: 61  TKNDIVLANRPRLLFGKHIGYNHTTMVGSPYGDHWRNLRRIGAIEIFSASRLNKFAGTRK 120
           TKND+VLANRP  L GKHIGYN TTMVG+ YGD WRNLRRIG IEIFS+ RLN F   R+
Sbjct: 100 TKNDVVLANRPEFLVGKHIGYNSTTMVGAAYGDSWRNLRRIGTIEIFSSLRLNSFVSIRQ 159

Query: 121 DEVERLLKKLSRNSMHGYSKVEMQSSISELTFNISMRMAAGKRYFGDEVRDVEEARQSRE 180
           DE+ RL+  L++NS HG+ KVEM+     LT N  +RM AGKR++GD   +  EA+  R+
Sbjct: 160 DEIRRLIICLAKNSQHGFVKVEMKPLFMCLTINNIIRMVAGKRFYGDGTENDNEAKHVRQ 219

Query: 181 LIKQIVSMGGVSNPGDFIPMMNWVPNGYKRKVSRLGKRMDRFLQGLIDEHRSNKEEERNT 240
           LI ++V  GG  N  D+ P++ +V N Y++ V +L  R+D FLQ L++E R  K +  NT
Sbjct: 220 LIAEVVVSGGAGNAADYFPILRYVTN-YEKHVKKLAGRVDEFLQSLVNEKRVEKVKG-NT 279

Query: 241 MIDHLLSLQETEPEYYGDEIIKGIVLVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREE 300
           MIDHLLSLQET+P+YY D IIKGI+LV++LAGTDTSA T+EWA+++LLN+PEVL+KA+ E
Sbjct: 280 MIDHLLSLQETQPDYYTDVIIKGIILVMILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTE 339

Query: 301 LDNQIGEKQLAEELDVSKLPYLQGIIYETLRLNPAAPMLVPHLTSNNCTISEYEIPRDTI 360
           +D+QIG  +L EE D+ KLPYLQ I+ ETLRL P APML+PHL S +C +  Y++PR TI
Sbjct: 340 IDDQIGVDRLVEEQDIVKLPYLQHIVSETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTI 399

Query: 361 VLVNAWAIHRDPSQWEEPTLFKPERHQNLESSDHHQIPKMIPFGVGRRACPGSSMAQRVV 420
           +LVNAWAIHRDP  WEEP  FKPER +        +  K++PFG+GRR+CPGS +AQR+V
Sbjct: 400 ILVNAWAIHRDPKLWEEPEKFKPERFEK-----KGEDKKLMPFGIGRRSCPGSGLAQRLV 459

Query: 421 GLTLATLIQCYEWERIGEEKVDMTEG-RGVTMPKVVPLEAMCKARPIVHNIFN 467
            L L +L+QC+EWER+ E+ +DM E  +G TM K   L+AMCKARPIVH + +
Sbjct: 460 TLALGSLVQCFEWERVEEKYLDMRESEKGTTMRKATSLQAMCKARPIVHKVLD 505

[Close]

BLAST of Cucsa.102750.1 vs. TAIR10
Match: AT4G37320.1 (AT4G37320.1 cytochrome P450, family 81, subfamily D, polypeptide 5)

HSP 1 Score: 535.0 bits (1377), Expect = 4.5e-152
Identity = 257/467 (55.03%), Postives = 343/467 (73.45%), Query Frame = 1


Query: 1   PPS----LPIIGHLHYIKIPLHRTLHKLSAKYG--PLISLRFGSRLVVVVSSLPAVEECF 60
           PPS    LP+IGHLH +K P+HRT H +S   G  P+  LR G+RLV V+SS    EECF
Sbjct: 32  PPSPAYPLPVIGHLHLLKQPVHRTFHSISKSLGNAPIFHLRLGNRLVYVISSHSIAEECF 91

Query: 61  TKNDIVLANRPRLLFGKHIGYNHTTMVGSPYGDHWRNLRRIGAIEIFSASRLNKFAGTRK 120
           TKND+VLANRP ++  KH+GYN T M+ + YGDHWRNLRRI A+EIFS+ R++ F+  RK
Sbjct: 92  TKNDVVLANRPDIIMAKHVGYNFTNMIAASYGDHWRNLRRIAAVEIFSSHRISTFSSIRK 151

Query: 121 DEVERLLKKLSRNSMHGYSKVEMQSSISELTFNISMRMAAGKRYFGDEVRDVEEARQSRE 180
           DE+ RL+  LSR+S+HG+ +VE++S ++ L FN  + M AGKRY+G    D +EA+  RE
Sbjct: 152 DEIRRLITHLSRDSLHGFVEVELKSLLTNLAFNNIIMMVAGKRYYGTGTEDNDEAKLVRE 211

Query: 181 LIKQIVSMGGVSNPGDFIPMMNWVPNGYKRKVSRLGKRMDRFLQGLIDEHRSNKEEERNT 240
           LI +I++  G  N  D++P +NWV N ++ +   LG R+DR LQ L+DE R+ KE+ + T
Sbjct: 212 LIAEIMAGAGSGNLADYLPSINWVTN-FENQTKILGNRLDRVLQKLVDEKRAEKEKGQ-T 271

Query: 241 MIDHLLSLQETEPEYYGDEIIKGIVLVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREE 300
           +IDHLLS QETEPEYY D IIKGI+L L+LAGTDTS+VT+EWA+++LLN+PE+L+KAR E
Sbjct: 272 LIDHLLSFQETEPEYYTDVIIKGIILALVLAGTDTSSVTLEWAMSNLLNHPEILEKARAE 331

Query: 301 LDNQIGEKQLAEELDVSKLPYLQGIIYETLRLNPAAPMLVPHLTSNNCTISEYEIPRDTI 360
           +D++IG  +L EE D+  L YLQ I+ ETLRL PA P+L+PH +S+ C ++ Y++PR T+
Sbjct: 332 IDDKIGSDRLVEESDIVNLHYLQNIVSETLRLYPAVPLLLPHFSSDECKVAGYDMPRRTL 391

Query: 361 VLVNAWAIHRDPSQWEEPTLFKPERHQNLESSDHHQIPKMIPFGVGRRACPGSSMAQRVV 420
           +L N WA+HRDP  WEEP  FKPER +        +  K++PFG+GRRACPG+ + +R+V
Sbjct: 392 LLTNVWAMHRDPGLWEEPERFKPERFEK-----EGEARKLMPFGMGRRACPGAELGKRLV 451

Query: 421 GLTLATLIQCYEWERIGEEKVDMTEGRGVTMPKVVPLEAMCKARPIV 462
            L L  LIQ +EWER+G E VDMTEG G+TMPK  PL AMCKAR IV
Sbjct: 452 SLALGCLIQSFEWERVGAELVDMTEGEGITMPKATPLRAMCKARAIV 491

[Close]

BLAST of Cucsa.102750.1 vs. NCBI nr
Match: gi|778718517|ref|XP_004141998.2| (PREDICTED: cytochrome P450 81E8-like [Cucumis sativus])

HSP 1 Score: 940.3 bits (2429), Expect = 1.3e-270
Identity = 466/466 (100.00%), Postives = 466/466 (100.00%), Query Frame = 1


Query: 1   PPSLPIIGHLHYIKIPLHRTLHKLSAKYGPLISLRFGSRLVVVVSSLPAVEECFTKNDIV 60
           PPSLPIIGHLHYIKIPLHRTLHKLSAKYGPLISLRFGSRLVVVVSSLPAVEECFTKNDIV
Sbjct: 33  PPSLPIIGHLHYIKIPLHRTLHKLSAKYGPLISLRFGSRLVVVVSSLPAVEECFTKNDIV 92

Query: 61  LANRPRLLFGKHIGYNHTTMVGSPYGDHWRNLRRIGAIEIFSASRLNKFAGTRKDEVERL 120
           LANRPRLLFGKHIGYNHTTMVGSPYGDHWRNLRRIGAIEIFSASRLNKFAGTRKDEVERL
Sbjct: 93  LANRPRLLFGKHIGYNHTTMVGSPYGDHWRNLRRIGAIEIFSASRLNKFAGTRKDEVERL 152

Query: 121 LKKLSRNSMHGYSKVEMQSSISELTFNISMRMAAGKRYFGDEVRDVEEARQSRELIKQIV 180
           LKKLSRNSMHGYSKVEMQSSISELTFNISMRMAAGKRYFGDEVRDVEEARQSRELIKQIV
Sbjct: 153 LKKLSRNSMHGYSKVEMQSSISELTFNISMRMAAGKRYFGDEVRDVEEARQSRELIKQIV 212

Query: 181 SMGGVSNPGDFIPMMNWVPNGYKRKVSRLGKRMDRFLQGLIDEHRSNKEEERNTMIDHLL 240
           SMGGVSNPGDFIPMMNWVPNGYKRKVSRLGKRMDRFLQGLIDEHRSNKEEERNTMIDHLL
Sbjct: 213 SMGGVSNPGDFIPMMNWVPNGYKRKVSRLGKRMDRFLQGLIDEHRSNKEEERNTMIDHLL 272

Query: 241 SLQETEPEYYGDEIIKGIVLVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDNQIG 300
           SLQETEPEYYGDEIIKGIVLVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDNQIG
Sbjct: 273 SLQETEPEYYGDEIIKGIVLVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDNQIG 332

Query: 301 EKQLAEELDVSKLPYLQGIIYETLRLNPAAPMLVPHLTSNNCTISEYEIPRDTIVLVNAW 360
           EKQLAEELDVSKLPYLQGIIYETLRLNPAAPMLVPHLTSNNCTISEYEIPRDTIVLVNAW
Sbjct: 333 EKQLAEELDVSKLPYLQGIIYETLRLNPAAPMLVPHLTSNNCTISEYEIPRDTIVLVNAW 392

Query: 361 AIHRDPSQWEEPTLFKPERHQNLESSDHHQIPKMIPFGVGRRACPGSSMAQRVVGLTLAT 420
           AIHRDPSQWEEPTLFKPERHQNLESSDHHQIPKMIPFGVGRRACPGSSMAQRVVGLTLAT
Sbjct: 393 AIHRDPSQWEEPTLFKPERHQNLESSDHHQIPKMIPFGVGRRACPGSSMAQRVVGLTLAT 452

Query: 421 LIQCYEWERIGEEKVDMTEGRGVTMPKVVPLEAMCKARPIVHNIFN 467
           LIQCYEWERIGEEKVDMTEGRGVTMPKVVPLEAMCKARPIVHNIFN
Sbjct: 453 LIQCYEWERIGEEKVDMTEGRGVTMPKVVPLEAMCKARPIVHNIFN 498

[Close]

BLAST of Cucsa.102750.1 vs. NCBI nr
Match: gi|778718513|ref|XP_011657880.1| (PREDICTED: cytochrome P450 81E8 [Cucumis sativus])

HSP 1 Score: 864.0 bits (2231), Expect = 1.2e-247
Identity = 427/466 (91.63%), Postives = 445/466 (95.49%), Query Frame = 1


Query: 1   PPSLPIIGHLHYIKIPLHRTLHKLSAKYGPLISLRFGSRLVVVVSSLPAVEECFTKNDIV 60
           PPSLPIIGHLHYIKIP HRTLHKLSAKYGP+ISL FGSRLVVVVSSLPAVEECFTKNDIV
Sbjct: 33  PPSLPIIGHLHYIKIPRHRTLHKLSAKYGPVISLWFGSRLVVVVSSLPAVEECFTKNDIV 92

Query: 61  LANRPRLLFGKHIGYNHTTMVGSPYGDHWRNLRRIGAIEIFSASRLNKFAGTRKDEVERL 120
           LANRPRLL GKH+GYNHTTMV SPYGDHWRNLRRIGAIEIFSASRLNKFAGTRKDEVERL
Sbjct: 93  LANRPRLLVGKHVGYNHTTMVASPYGDHWRNLRRIGAIEIFSASRLNKFAGTRKDEVERL 152

Query: 121 LKKLSRNSMHGYSKVEMQSSISELTFNISMRMAAGKRYFGDEVRDVEEARQSRELIKQIV 180
           LKKLSRNSM+G+SKVEMQSSISELTFNISMRMAAGKRYFG+EVRDVEEARQSRELIKQIV
Sbjct: 153 LKKLSRNSMYGFSKVEMQSSISELTFNISMRMAAGKRYFGEEVRDVEEARQSRELIKQIV 212

Query: 181 SMGGVSNPGDFIPMMNWVPNGYKRKVSRLGKRMDRFLQGLIDEHRSNKEEERNTMIDHLL 240
           SMGGVSNPGDFIPMMNW+PNG+KRKV R+ KRMD FLQGLIDEHRSNKEEERNTMIDHLL
Sbjct: 213 SMGGVSNPGDFIPMMNWIPNGFKRKVWRVAKRMDGFLQGLIDEHRSNKEEERNTMIDHLL 272

Query: 241 SLQETEPEYYGDEIIKGIVLVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDNQIG 300
           SLQETEPEYYGD+IIKGI+LVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELD QIG
Sbjct: 273 SLQETEPEYYGDQIIKGIILVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDTQIG 332

Query: 301 EKQLAEELDVSKLPYLQGIIYETLRLNPAAPMLVPHLTSNNCTISEYEIPRDTIVLVNAW 360
           EK+L EE DVSKLPYLQGII ETLRLNPAAPMLVPHLTSN+CTIS Y+IPRDTIVLVNAW
Sbjct: 333 EKRLVEESDVSKLPYLQGIISETLRLNPAAPMLVPHLTSNDCTISGYKIPRDTIVLVNAW 392

Query: 361 AIHRDPSQWEEPTLFKPERHQNLESSDHHQIPKMIPFGVGRRACPGSSMAQRVVGLTLAT 420
           AIHRDP QWEEPTLFKPERHQ  ES DHH I K+IPFGVGRRACPGS MAQRVVGLTLA 
Sbjct: 393 AIHRDPHQWEEPTLFKPERHQKSESIDHH-ISKLIPFGVGRRACPGSGMAQRVVGLTLAA 452

Query: 421 LIQCYEWERIGEEKVDMTEGRGVTMPKVVPLEAMCKARPIVHNIFN 467
           LIQCYEWERIG+EKVDM+EGRGVTMPK+VPLEAMCK RPI+HNIFN
Sbjct: 453 LIQCYEWERIGDEKVDMSEGRGVTMPKMVPLEAMCKPRPIIHNIFN 497

[Close]

BLAST of Cucsa.102750.1 vs. NCBI nr
Match: gi|659079573|ref|XP_008440328.1| (PREDICTED: isoflavone 2'-hydroxylase [Cucumis melo])

HSP 1 Score: 852.4 bits (2201), Expect = 3.6e-244
Identity = 416/466 (89.27%), Postives = 444/466 (95.28%), Query Frame = 1


Query: 1   PPSLPIIGHLHYIKIPLHRTLHKLSAKYGPLISLRFGSRLVVVVSSLPAVEECFTKNDIV 60
           PPSLPIIGHLHY+KIPLHRTL KLSAKYGP+ISL FGSRLVVVVSSLPAVEECFTKNDIV
Sbjct: 33  PPSLPIIGHLHYLKIPLHRTLQKLSAKYGPVISLWFGSRLVVVVSSLPAVEECFTKNDIV 92

Query: 61  LANRPRLLFGKHIGYNHTTMVGSPYGDHWRNLRRIGAIEIFSASRLNKFAGTRKDEVERL 120
           LANRPRLL GKH+GYNHTTMV SPYGDHWRNLRRIGAIEIFSASRLNKFAGTRKDEVERL
Sbjct: 93  LANRPRLLVGKHVGYNHTTMVASPYGDHWRNLRRIGAIEIFSASRLNKFAGTRKDEVERL 152

Query: 121 LKKLSRNSMHGYSKVEMQSSISELTFNISMRMAAGKRYFGDEVRDVEEARQSRELIKQIV 180
           LKKLSRNSM+G+SKVEMQS+I+ELTFNISMRMAAGKRYFG+EV DVEEARQSRELIKQIV
Sbjct: 153 LKKLSRNSMNGFSKVEMQSAIAELTFNISMRMAAGKRYFGEEVTDVEEARQSRELIKQIV 212

Query: 181 SMGGVSNPGDFIPMMNWVPNGYKRKVSRLGKRMDRFLQGLIDEHRSNKEEERNTMIDHLL 240
           S+GGVSNPGDFIP+MNW+PNG++RKVSRL KRMD FLQGLIDEHRSNK EERNTMIDHLL
Sbjct: 213 SLGGVSNPGDFIPLMNWIPNGFRRKVSRLAKRMDGFLQGLIDEHRSNKCEERNTMIDHLL 272

Query: 241 SLQETEPEYYGDEIIKGIVLVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDNQIG 300
           SLQE+EPEYYGD+IIKGI+LVLLLAGTDTSAVTIEWALAHLLNN EVLKKAREELD QIG
Sbjct: 273 SLQESEPEYYGDQIIKGIILVLLLAGTDTSAVTIEWALAHLLNNLEVLKKAREELDTQIG 332

Query: 301 EKQLAEELDVSKLPYLQGIIYETLRLNPAAPMLVPHLTSNNCTISEYEIPRDTIVLVNAW 360
           EK+L EE D+ KLPYLQGII ETLRLNPAAPMLVPHLTSN+CTIS Y+IPRDTIVLVNAW
Sbjct: 333 EKRLVEESDILKLPYLQGIISETLRLNPAAPMLVPHLTSNDCTISGYKIPRDTIVLVNAW 392

Query: 361 AIHRDPSQWEEPTLFKPERHQNLESSDHHQIPKMIPFGVGRRACPGSSMAQRVVGLTLAT 420
           AIHR+P+QWEEPTLFKPERHQ  ESS HHQIPK+IPFG+GRRACPGS MAQRVVGLTLA 
Sbjct: 393 AIHREPNQWEEPTLFKPERHQKSESSSHHQIPKLIPFGIGRRACPGSGMAQRVVGLTLAA 452

Query: 421 LIQCYEWERIGEEKVDMTEGRGVTMPKVVPLEAMCKARPIVHNIFN 467
           LIQCYEWERIGEEKVDM EGRGVTMPK++PLEAMCK RPI+H+IFN
Sbjct: 453 LIQCYEWERIGEEKVDMAEGRGVTMPKLLPLEAMCKPRPIIHHIFN 498

[Close]

BLAST of Cucsa.102750.1 vs. NCBI nr
Match: gi|778722417|ref|XP_004141999.2| (PREDICTED: cytochrome P450 81E8-like [Cucumis sativus])

HSP 1 Score: 820.8 bits (2119), Expect = 1.2e-234
Identity = 407/467 (87.15%), Postives = 434/467 (92.93%), Query Frame = 1


Query: 1   PPSLPIIGHLHYIKIPLHRTLHKLSAKYGPLISLRFGSRLVVVVSSLPAVEECFTKNDIV 60
           PPSLPIIGHLHY+KIP H+TLHKLSA+YGP+ISL  GSRLVVVVSSLPAVEECFTKNDIV
Sbjct: 33  PPSLPIIGHLHYLKIPHHQTLHKLSAQYGPVISLWLGSRLVVVVSSLPAVEECFTKNDIV 92

Query: 61  LANRPRLLFGKHIGYNHTTMVGSPYGDHWRNLRRIGAIEIFSASRLNKFAGTRKDEVERL 120
           LANRPRLL GKHI YN+TTMV SPYGDHWRNLRRIGAIEIFSASRLNKFAG+RKDEVERL
Sbjct: 93  LANRPRLLVGKHISYNYTTMVSSPYGDHWRNLRRIGAIEIFSASRLNKFAGSRKDEVERL 152

Query: 121 LKKLSRNSMHGYSKVEMQSSISELTFNISMRMAAGKRYFGDEVRDVEEARQSRELIKQIV 180
           LKKLSRNSM+G+SKVEMQS+ISE TFNISMRMAAGKRYFG+EVRD+EEARQSRELIKQIV
Sbjct: 153 LKKLSRNSMYGFSKVEMQSAISERTFNISMRMAAGKRYFGEEVRDLEEARQSRELIKQIV 212

Query: 181 SMGGVSNPGDFIPMMNWVPNGYKRKVSRLGKRMDRFLQGLIDEHRSNKEEERNTMIDHLL 240
           SMGGVS PGDFIPMMNW+PNG+KRKV R+ KRMD FLQGLIDEHRSNKEEERNT+I HLL
Sbjct: 213 SMGGVSYPGDFIPMMNWIPNGFKRKVWRVAKRMDAFLQGLIDEHRSNKEEERNTVIGHLL 272

Query: 241 SLQETEPEYYGDEIIKGIVLVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDNQIG 300
           SLQE EPEYYGDEIIKGIVL LLLAG DTSAVTIEW LAHLLNNP+VLKKAREE+D QIG
Sbjct: 273 SLQEMEPEYYGDEIIKGIVL-LLLAGVDTSAVTIEWILAHLLNNPDVLKKAREEIDAQIG 332

Query: 301 EKQLAEELDVSKLPYLQGIIYETLRLNPAAPMLVPHLTSNNCTISEYEIPRDTIVLVNAW 360
           EK+L EE D+ KLPYLQGII ETLRLNP APMLVPHLTS++CTIS Y+IPRDTIVLVNAW
Sbjct: 333 EKRLVEESDIPKLPYLQGIISETLRLNPTAPMLVPHLTSHDCTISGYKIPRDTIVLVNAW 392

Query: 361 AIHRDPSQWEEPTLFKPERHQ-NLESSDHHQIPKMIPFGVGRRACPGSSMAQRVVGLTLA 420
           AIHRDP+QWEEP LFKPERHQ + ES DHHQIPK+IPFGVGRRACPGS MAQRVVGLTLA
Sbjct: 393 AIHRDPNQWEEPMLFKPERHQKSSESIDHHQIPKLIPFGVGRRACPGSGMAQRVVGLTLA 452

Query: 421 TLIQCYEWERIGEEKVDMTEGRGVTMPKVVPLEAMCKARPIVHNIFN 467
            LIQCYEWERIGEEKVDM EGRG TMPKV+PLEAMCK R I+HNIFN
Sbjct: 453 ALIQCYEWERIGEEKVDMAEGRGATMPKVLPLEAMCKPRSIIHNIFN 498

[Close]

BLAST of Cucsa.102750.1 vs. NCBI nr
Match: gi|778718524|ref|XP_004141996.2| (PREDICTED: cytochrome P450 81E8-like [Cucumis sativus])

HSP 1 Score: 684.5 bits (1765), Expect = 1.3e-193
Identity = 320/465 (68.82%), Postives = 403/465 (86.67%), Query Frame = 1


Query: 1   PPSLPIIGHLHYIKIPLHRTLHKLSAKYGPLISLRFGSRLVVVVSSLPAVEECFTKNDIV 60
           PPS P+IGHLH +K P+HRT   LSAKYGP++SLR GS+L VV+SS  AVE+CFTKND+V
Sbjct: 38  PPSFPVIGHLHLLKKPIHRTFSNLSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVV 97

Query: 61  LANRPRLLFGKHIGYNHTTMVGSPYGDHWRNLRRIGAIEIFSASRLNKFAGTRKDEVERL 120
           LANRPRLL GK+IGYN+TTM+G+PYGDHWRNLRRIGAIEIFS SR+NKF   R+DEV R+
Sbjct: 98  LANRPRLLVGKYIGYNYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRI 157

Query: 121 LKKLSRNSMHGYSKVEMQSSISELTFNISMRMAAGKRYFGDEVRDVEEARQSRELIKQIV 180
           ++KLS NS+H +SKVE+QS++SELTFNI+MRMAAGKRY+G++V + EEAR+ RELIK+IV
Sbjct: 158 VRKLSHNSIHQFSKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIV 217

Query: 181 SMGGVSNPGDFIPMMNWVPNGYKRKVSRLGKRMDRFLQGLIDEHRSNKEEERNTMIDHLL 240
           ++GGVSNPGDF+P++NW+  G +RK+ +LGK++D FLQGLID+HR  KEE RNTMIDHLL
Sbjct: 218 AVGGVSNPGDFLPVLNWMSKGIERKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLL 277

Query: 241 SLQETEPEYYGDEIIKGIVLVLLLAGTDTSAVTIEWALAHLLNNPEVLKKAREELDNQIG 300
           SLQ++EP++Y D+IIKG +L+LL AG+DTSAVT+EWAL+HLLNNP+VLKKA+EE+D QIG
Sbjct: 278 SLQQSEPDHYNDQIIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIG 337

Query: 301 EKQLAEELDVSKLPYLQGIIYETLRLNPAAPMLVPHLTSNNCTISEYEIPRDTIVLVNAW 360
           +++L EE D+SKLPYLQ I+ ETLRLNPAAPMLVPH  +N+C I  Y++PRDT+VLVNAW
Sbjct: 338 QERLVEESDISKLPYLQRIVSETLRLNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAW 397

Query: 361 AIHRDPSQWEEPTLFKPERHQNLESSDHHQIPKMIPFGVGRRACPGSSMAQRVVGLTLAT 420
           AIHRD ++WE+   FKPER++  E+ + H   K +PFG+GRRACPGS+MA RV+GLTLAT
Sbjct: 398 AIHRDSNEWEDCMSFKPERYEKSEAVEIH---KFLPFGLGRRACPGSAMAHRVMGLTLAT 457

Query: 421 LIQCYEWERIGEEKVDMTEGRGVTMPKVVPLEAMCKARPIVHNIF 466
           LIQC+EW++IGEE +DM E  G  M K+VPLEAMCKARPI++NIF
Sbjct: 458 LIQCFEWKKIGEEDIDMKELSGAIMTKMVPLEAMCKARPIINNIF 499

The following BLAST results are available for this feature:

BLAST of Cucsa.102750.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
C81E8_MEDTR	6.8e-158	58.13	Cytochrome P450 81E8 OS=Medicago truncatula GN=CYP81E8 PE=2 SV=1	[more]
C81E9_MEDTR	5.4e-155	56.26	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula GN=CYP81E9 PE=1 SV=1	[more]
C8D11_ARATH	4.7e-151	56.87	Cytochrome P450 81D11 OS=Arabidopsis thaliana GN=CYP81D11 PE=2 SV=1	[more]
C81E7_MEDTR	3.7e-148	52.99	Isoflavone 2'-hydroxylase OS=Medicago truncatula GN=CYP81E7 PE=1 SV=1	[more]
C81D1_ARATH	2.0e-146	53.78	Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CYP81D1 PE=2 SV=1	[more]

BLAST of Cucsa.102750.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KLF2_CUCSA	9.2e-271	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G492220 PE=3 SV=1	[more]
A0A0A0KJM0_CUCSA	8.4e-248	91.63	Cytochrome P450 OS=Cucumis sativus GN=Csa_6G492230 PE=3 SV=1	[more]
A0A0A0KFV2_CUCSA	9.1e-194	68.82	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G492250 PE=3 SV=1	[more]
A0A061EFX4_THECC	7.0e-178	66.17	Cytochrome P450, family 81, subfamily D, polypeptide 8, putative OS=Theobroma ca...	[more]
W9QYM5_9ROSA	2.7e-177	66.02	Isoflavone 2'-hydroxylase OS=Morus notabilis GN=L484_009304 PE=3 SV=1	[more]

BLAST of Cucsa.102750.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G37340.1	1.5e-155	57.08	cytochrome P450, family 81, subfamily D, polypeptide 3	[more]
AT4G37360.1	2.8e-154	56.81	cytochrome P450, family 81, subfamily D, polypeptide 2	[more]
AT4G37370.1	4.8e-154	56.87	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]
AT3G28740.1	2.7e-152	56.87	Cytochrome P450 superfamily protein	[more]
AT4G37320.1	4.5e-152	55.03	cytochrome P450, family 81, subfamily D, polypeptide 5	[more]

BLAST of Cucsa.102750.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778718517\|ref\|XP_004141998.2\|	1.3e-270	100.00	PREDICTED: cytochrome P450 81E8-like [Cucumis sativus]	[more]
gi\|778718513\|ref\|XP_011657880.1\|	1.2e-247	91.63	PREDICTED: cytochrome P450 81E8 [Cucumis sativus]	[more]
gi\|659079573\|ref\|XP_008440328.1\|	3.6e-244	89.27	PREDICTED: isoflavone 2'-hydroxylase [Cucumis melo]	[more]
gi\|778722417\|ref\|XP_004141999.2\|	1.2e-234	87.15	PREDICTED: cytochrome P450 81E8-like [Cucumis sativus]	[more]
gi\|778718524\|ref\|XP_004141996.2\|	1.3e-193	68.82	PREDICTED: cytochrome P450 81E8-like [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR001128	Cyt_P450
IPR002401	Cyt_P450_E_grp-I
IPR017972	Cyt_P450_CS

Vocabulary: **Molecular Function**
Term	Definition
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0005506	iron ion binding
GO:0020037	heme binding

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0004672	protein kinase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsa.102750	Cucsa.102750	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsa.102750.1	Cucsa.102750.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.102750.1.CDS.2	Cucsa.102750.1.CDS.2	CDS
Cucsa.102750.1.CDS.1	Cucsa.102750.1.CDS.1	CDS

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 318..329 score: 1.1E-11coord: 265..282 score: 1.1E-11coord: 404..415 score: 1.1E-11coord: 395..404 score: 1.1
IPR001128	Cytochrome P450	GENE3D	G3DSA:1.10.630.10		coord: 1..458 score: 3.8E
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 1..447 score: 1.0
IPR001128	Cytochrome P450	unknown	SSF48264	Cytochrome P450	coord: 1..458 score: 1.96E
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 144..162 score: 5.1E-47coord: 254..271 score: 5.1E-47coord: 358..382 score: 5.1E-47coord: 394..404 score: 5.1E-47coord: 25..44 score: 5.1E-47coord: 49..70 score: 5.1E-47coord: 404..427 score: 5.1E-47coord: 274..300 score: 5.1E-47coord: 317..335 score: 5.1
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 397..406 scor
None	No IPR available	unknown	Coil	Coil	coord: 288..308 scor
None	No IPR available	PANTHER	PTHR24298	FAMILY NOT NAMED	coord: 1..462 score: 6.7E
None	No IPR available	PANTHER	PTHR24298:SF61	CYTOCHROME P450 81D1-RELATED	coord: 1..462 score: 6.7E