Cucsa.102320.4 (mRNA) Cucumber (Gy14) v1

NameCucsa.102320.4
TypemRNA
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionProtein of unknown function, DUF547
Locationscaffold00927 : 1344166 .. 1347334 (-)
Sequence length2293
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTGTTTCTTTTTCTTTTTCTTTCACCTTTTTTATTGAAACGTCTTATTGTACCACTTTAGATTGCTTTACTCCCTCCATTTCGATTTATTTGGTTTTAGCCTTTCACTTGATTATATTTTTTCCCATCAAATTTCCTCTGCAATGATTTTGGTATTCACACGACAGATATGATTGTAATTCTTGTTCTTTCTGTTATACATTATGTTCATAAACTTTTGTGACTGTGTTTCCTTTGTTGAATTTGGATAGAAGGTGAAGAAATTAGATAGCGGAAGGAGCAAGACGATGGGTAATGACAAACCTGGGACTATCATAAATCGGCGCAAATTAAACAGAGAGAGAAAAATAGCATTGTTACAAGATGTAATTATGTCCTTCTCCAACTGCCATTTTAGGGAAATTGCCTGTTTTGAACATGTTTGATGGTTATGCAATTTTGAAATTTGCAGGTTGATAAGCTGAAGAAGAAGCTCAGACATGAAGAAAATGTTCACAAGGCATTAGAAAGAGCTTTTACTAGACCTTTAGGAGCTCTCCCTCGTCTTCCTCCATATCTTCCTCCTTATGTTAGTAAAACTTGCAAATTCCATATATTACATCCCTTTTCTCTCTTTTTCATTCTTTTTTCCAGTTCTTCATAGAAAGAATGGAAAAGGGTTGGGCTGAAGCACTTCAATTTTGAAAATTTATTTTTCCTACACCTTGTTGCAGATACTAGAACTTCTTGCTGAAGTAGCTGTTTTGGAGGAAGAGGTTGTTAGACTAGAAGAACAGGTTGTGAATTTTAGACAAGGATTATATCAGGAAGCTGTGTATGTTTCATCCTCCTCTAGGAATTCAGATATTTCCACAGACACAATGGAACCTATCTCAACAAGAATCGCGAAGCATCGGAGAACGAAGTCGTATTGCCAAAATGAGTTCAATTCTGCAAACTCAATAGCCAGGCTGCAGCCTTCACTGGCCAGATGTTCTTCGAGTCGAAAGTTATTGTCGAACGACACTTTCTTTGATCGTAATGGTAACGGTTCTAACAGGTTTGCCAATGGGAAACATGTTCCCGGGAAATCAAGCTCCTTTTTATTTCTTCCAGAAGATGGACTGGGAAAAGAGAATCAATCATATGCAAATACTGTAAAAAATAAGCCATCTCCTGAAAAGAAAGTTGATAGAATTATAAGCCCATTGAAGAAGTCTCCTCTAAAACAAGAATTTTTGGAGAAGAACTCAAGTCCTATGAAGTCTCAGGTTTTTGCTTGATACCATATCGAATTTATCATTATCACCACCTTAATAAATTTCAAATTACTCATTATTTCCTTTTAATTTTCCAGCTGGAGTTTCGATTGGAACGAGAAAGAGCAAAGGACAATTCATCTAATTTGTCTGATGATACAGAGGCTAGTAGCTCACCAAACAAAATTTCTGAAGACATTGTGAAGTGTTTATCATCCATTTTCATAAGACTGAGTTCATCAAAAGACAAAGCAACGGACTCATCAGATACATCAAGTGGACCTGCGGAATTGCAAGATCCTTACGATGCTTGTTCAGATTTCAAACCTAGAAACATTGGTCCCTACAGACATCTCTGTGCAATTGAAGCTAGTTCCGTAGATCTCGATCGATCAACTAATGCAGTTTTTCTAATTCATAGGCTAAAGTAAGTTCTCAGGATGCCTCATCTTATTCTTGGCTGAAATTTTTGTTGATTATTTTATCTTAATGGCGCAATTCATTTTACTCCACAGAAACTTGTTCCGGAGACTCGCCTCGGTGAACTTGGCAGGTCTTAACCATCAGGAGAAGCTAGCTTTCTGGATCAACACCTACAATTCCTGCATGATGAACGTGAGTTACTAACGAACAATTTTATGTCAGGAGGATGGCAATATTAAAATCTTCACTAACTTGTTAATTAGAGGCCTAAATCAAGAAACTGAGGTACCTGAATTTTGTGACTTTAGGCATTTTTAGAGCAGGGGATACCTGAGACTCATGAAAGAGTTGTAACATTGATGCAAAAGGTAAAATCAGAATGCCATCTTCTTTGAATTTGCTATCTGCCTGATCATCAAACGTTCATTACATTTAAGCTGGAAACTTTTCTTCTCCTGATAGGCAACAATTATTGTGGGAGGGCACCTTCTAAATGCAATAACAATTGAGCATTTCATCTTGAGACTTCCTTATCACCTGAAGTTCGTAAGTCTATCCTTTCTAACTCCATTGAACTTTGTGACATCCAGATTTCCCAAATTTCTACTGGTTTCAAATCTTATAAAGTCCAATGCTAAATACTTGCTGCATTTTTGTCTATAAACTTCAGACCTGCCCAAAAGCAGTAAAGAACGACGAGATGAGAGCACGCAGTGTGTTTGGATTGGAATATTCCGAACCCTTGATTACGTTTGCACTCTGTTGTGGAAGCTGGTCGTCTCCCGCCGTAAGTATGCTAAATTTGCATATGGTAGTTAGATAAAACATTGTACATCTTAGTATAACTCTGCTAACAACTTTGGTTGATAGTAAGTTTGATTTAAATGAAACTTCTACTAATCAACGGGTTGTTGCGAAATGAAGGTGAGAGTGTATTCAGGATGTAAAGTTGAAGAGGAGTTAGAAGTAGCCAAGAGGGAATATTTACAGGCAGCAGTTGGGATTTCAAAGACAAACAACAAGTTGATGATTCCAAAGGTTTTGGATTGGTATTTACTTGACTTTGCAAAGGATTTGGAATCAATGTTGGATTGGATTTGCTTACAACTTCCAAATGAACTAAGAATTGAAGCAGTTAAATGCCTTGAGAGGAAAGGAAGAGAGCCTCTCTCACAACTAGTGCAAGTTATGCCATATAATTTCAGCTTCAGAATGCTCTTACACAGATGAATTGATTAACTTTTTCATCTTATTTATTGATTGATTTATTTATTCATTTGGTTCGTGCTTTTTCAATCCTCCAATTCTCAATGTTCTCTGAATGTTGAATGTGCTGCTGAGCTTATACTAAACATAGAAGACAGTTATGTTATAGAATCAATACACAAAGCATAGCTGTAAACTTGGGGATTTTGGGCAGTTTCTAGTTTTTAATTAACTTTTTTTTTTGTTATGATTTTGTATTGATAATGGGAATTAGTAGAATAAAACTTGGTTCTGGGGAATA

mRNA sequence

GTTTGTTTCTTTTTCTTTTTCTTTCACCTTTTTTATTGAAACGTCTTATTGTACCACTTTAGATTGCTTTACTCCCTCCATTTCGATTTATTTGGTTTTAGCCTTTCACTTGATTATATTTTTTCCCATCAAATTTCCTCTGCAATGATTTTGGTATTCACACGACAGATATGATTGTAATTCTTGTTCTTTCTGTTATACATTATGTTCATAAACTTTTGTGACTGTGTTTCCTTTGTTGAATTTGGATAGAAGGTGAAGAAATTAGATAGCGGAAGGAGCAAGACGATGGGTAATGACAAACCTGGGACTATCATAAATCGGCGCAAATTAAACAGAGAGAGAAAAATAGCATTGTTACAAGATGTTGATAAGCTGAAGAAGAAGCTCAGACATGAAGAAAATGTTCACAAGGCATTAGAAAGAGCTTTTACTAGACCTTTAGGAGCTCTCCCTCGTCTTCCTCCATATCTTCCTCCTTATATACTAGAACTTCTTGCTGAAGTAGCTGTTTTGGAGGAAGAGGTTGTTAGACTAGAAGAACAGGTTGTGAATTTTAGACAAGGATTATATCAGGAAGCTGTGTATGTTTCATCCTCCTCTAGGAATTCAGATATTTCCACAGACACAATGGAACCTATCTCAACAAGAATCGCGAAGCATCGGAGAACGAAGTCGTATTGCCAAAATGAGTTCAATTCTGCAAACTCAATAGCCAGGCTGCAGCCTTCACTGGCCAGATGTTCTTCGAGTCGAAAGTTATTGTCGAACGACACTTTCTTTGATCGTAATGGTAACGGTTCTAACAGGTTTGCCAATGGGAAACATGTTCCCGGGAAATCAAGCTCCTTTTTATTTCTTCCAGAAGATGGACTGGGAAAAGAGAATCAATCATATGCAAATACTGTAAAAAATAAGCCATCTCCTGAAAAGAAAGTTGATAGAATTATAAGCCCATTGAAGAAGTCTCCTCTAAAACAAGAATTTTTGGAGAAGAACTCAAGTCCTATGAAGTCTCAGCTGGAGTTTCGATTGGAACGAGAAAGAGCAAAGGACAATTCATCTAATTTGTCTGATGATACAGAGGCTAGTAGCTCACCAAACAAAATTTCTGAAGACATTGTGAAGTGTTTATCATCCATTTTCATAAGACTGAGTTCATCAAAAGACAAAGCAACGGACTCATCAGATACATCAAGTGGACCTGCGGAATTGCAAGATCCTTACGATGCTTGTTCAGATTTCAAACCTAGAAACATTGGTCCCTACAGACATCTCTGTGCAATTGAAGCTAGTTCCGTAGATCTCGATCGATCAACTAATGCAGTTTTTCTAATTCATAGGCTAAAAAACTTGTTCCGGAGACTCGCCTCGGTGAACTTGGCAGGTCTTAACCATCAGGAGAAGCTAGCTTTCTGGATCAACACCTACAATTCCTGCATGATGAACGCATTTTTAGAGCAGGGGATACCTGAGACTCATGAAAGAGTTGTAACATTGATGCAAAAGGCAACAATTATTGTGGGAGGGCACCTTCTAAATGCAATAACAATTGAGCATTTCATCTTGAGACTTCCTTATCACCTGAAGTTCACCTGCCCAAAAGCAGTAAAGAACGACGAGATGAGAGCACGCAGTGTGTTTGGATTGGAATATTCCGAACCCTTGATTACGTTTGCACTCTGTTGTGGAAGCTGGTCGTCTCCCGCCGTGAGAGTGTATTCAGGATGTAAAGTTGAAGAGGAGTTAGAAGTAGCCAAGAGGGAATATTTACAGGCAGCAGTTGGGATTTCAAAGACAAACAACAAGTTGATGATTCCAAAGGTTTTGGATTGGTATTTACTTGACTTTGCAAAGGATTTGGAATCAATGTTGGATTGGATTTGCTTACAACTTCCAAATGAACTAAGAATTGAAGCAGTTAAATGCCTTGAGAGGAAAGGAAGAGAGCCTCTCTCACAACTAGTGCAAGTTATGCCATATAATTTCAGCTTCAGAATGCTCTTACACAGATGAATTGATTAACTTTTTCATCttatttattgattgatttatttattcatttGGTTCGTGCTTTTTCAATCCTCCAATTCTCAATGTTCTCTGAATGTTGAATGTGCTGCTGAGCTTATACTAAACATAGAAGACAGTTATGTTATAGAATCAATACACAAAGCATAGCTGTAAACTTGGGGATTTTGGGCAGTTTCTAGTTTTTAATTAACTTTTTTTTTTGTTATGATTTTGTATTGATAATGGGAATTAGTAGAATAAAACTTGGTTCTGGGGAATA

Coding sequence (CDS)

ATGGGTAATGACAAACCTGGGACTATCATAAATCGGCGCAAATTAAACAGAGAGAGAAAAATAGCATTGTTACAAGATGTTGATAAGCTGAAGAAGAAGCTCAGACATGAAGAAAATGTTCACAAGGCATTAGAAAGAGCTTTTACTAGACCTTTAGGAGCTCTCCCTCGTCTTCCTCCATATCTTCCTCCTTATATACTAGAACTTCTTGCTGAAGTAGCTGTTTTGGAGGAAGAGGTTGTTAGACTAGAAGAACAGGTTGTGAATTTTAGACAAGGATTATATCAGGAAGCTGTGTATGTTTCATCCTCCTCTAGGAATTCAGATATTTCCACAGACACAATGGAACCTATCTCAACAAGAATCGCGAAGCATCGGAGAACGAAGTCGTATTGCCAAAATGAGTTCAATTCTGCAAACTCAATAGCCAGGCTGCAGCCTTCACTGGCCAGATGTTCTTCGAGTCGAAAGTTATTGTCGAACGACACTTTCTTTGATCGTAATGGTAACGGTTCTAACAGGTTTGCCAATGGGAAACATGTTCCCGGGAAATCAAGCTCCTTTTTATTTCTTCCAGAAGATGGACTGGGAAAAGAGAATCAATCATATGCAAATACTGTAAAAAATAAGCCATCTCCTGAAAAGAAAGTTGATAGAATTATAAGCCCATTGAAGAAGTCTCCTCTAAAACAAGAATTTTTGGAGAAGAACTCAAGTCCTATGAAGTCTCAGCTGGAGTTTCGATTGGAACGAGAAAGAGCAAAGGACAATTCATCTAATTTGTCTGATGATACAGAGGCTAGTAGCTCACCAAACAAAATTTCTGAAGACATTGTGAAGTGTTTATCATCCATTTTCATAAGACTGAGTTCATCAAAAGACAAAGCAACGGACTCATCAGATACATCAAGTGGACCTGCGGAATTGCAAGATCCTTACGATGCTTGTTCAGATTTCAAACCTAGAAACATTGGTCCCTACAGACATCTCTGTGCAATTGAAGCTAGTTCCGTAGATCTCGATCGATCAACTAATGCAGTTTTTCTAATTCATAGGCTAAAAAACTTGTTCCGGAGACTCGCCTCGGTGAACTTGGCAGGTCTTAACCATCAGGAGAAGCTAGCTTTCTGGATCAACACCTACAATTCCTGCATGATGAACGCATTTTTAGAGCAGGGGATACCTGAGACTCATGAAAGAGTTGTAACATTGATGCAAAAGGCAACAATTATTGTGGGAGGGCACCTTCTAAATGCAATAACAATTGAGCATTTCATCTTGAGACTTCCTTATCACCTGAAGTTCACCTGCCCAAAAGCAGTAAAGAACGACGAGATGAGAGCACGCAGTGTGTTTGGATTGGAATATTCCGAACCCTTGATTACGTTTGCACTCTGTTGTGGAAGCTGGTCGTCTCCCGCCGTGAGAGTGTATTCAGGATGTAAAGTTGAAGAGGAGTTAGAAGTAGCCAAGAGGGAATATTTACAGGCAGCAGTTGGGATTTCAAAGACAAACAACAAGTTGATGATTCCAAAGGTTTTGGATTGGTATTTACTTGACTTTGCAAAGGATTTGGAATCAATGTTGGATTGGATTTGCTTACAACTTCCAAATGAACTAAGAATTGAAGCAGTTAAATGCCTTGAGAGGAAAGGAAGAGAGCCTCTCTCACAACTAGTGCAAGTTATGCCATATAATTTCAGCTTCAGAATGCTCTTACACAGATGA

Protein sequence

MGNDKPGTIINRRKLNRERKIALLQDVDKLKKKLRHEENVHKALERAFTRPLGALPRLPPYLPPYILELLAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYVSSSSRNSDISTDTMEPISTRIAKHRRTKSYCQNEFNSANSIARLQPSLARCSSSRKLLSNDTFFDRNGNGSNRFANGKHVPGKSSSFLFLPEDGLGKENQSYANTVKNKPSPEKKVDRIISPLKKSPLKQEFLEKNSSPMKSQLEFRLERERAKDNSSNLSDDTEASSSPNKISEDIVKCLSSIFIRLSSSKDKATDSSDTSSGPAELQDPYDACSDFKPRNIGPYRHLCAIEASSVDLDRSTNAVFLIHRLKNLFRRLASVNLAGLNHQEKLAFWINTYNSCMMNAFLEQGIPETHERVVTLMQKATIIVGGHLLNAITIEHFILRLPYHLKFTCPKAVKNDEMRARSVFGLEYSEPLITFALCCGSWSSPAVRVYSGCKVEEELEVAKREYLQAAVGISKTNNKLMIPKVLDWYLLDFAKDLESMLDWICLQLPNELRIEAVKCLERKGREPLSQLVQVMPYNFSFRMLLHR*
BLAST of Cucsa.102320.4 vs. TrEMBL
Match: A0A0A0KLB6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G490260 PE=4 SV=1)

HSP 1 Score: 1143.6 bits (2957), Expect = 0.0e+00
Identity = 575/575 (100.00%), Postives = 575/575 (100.00%), Query Frame = 1

Query: 1   MGNDKPGTIINRRKLNRERKIALLQDVDKLKKKLRHEENVHKALERAFTRPLGALPRLPP 60
           MGNDKPGTIINRRKLNRERKIALLQDVDKLKKKLRHEENVHKALERAFTRPLGALPRLPP
Sbjct: 32  MGNDKPGTIINRRKLNRERKIALLQDVDKLKKKLRHEENVHKALERAFTRPLGALPRLPP 91

Query: 61  YLPPYILELLAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYVSSSSRNSDISTDTMEPIST 120
           YLPPYILELLAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYVSSSSRNSDISTDTMEPIST
Sbjct: 92  YLPPYILELLAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYVSSSSRNSDISTDTMEPIST 151

Query: 121 RIAKHRRTKSYCQNEFNSANSIARLQPSLARCSSSRKLLSNDTFFDRNGNGSNRFANGKH 180
           RIAKHRRTKSYCQNEFNSANSIARLQPSLARCSSSRKLLSNDTFFDRNGNGSNRFANGKH
Sbjct: 152 RIAKHRRTKSYCQNEFNSANSIARLQPSLARCSSSRKLLSNDTFFDRNGNGSNRFANGKH 211

Query: 181 VPGKSSSFLFLPEDGLGKENQSYANTVKNKPSPEKKVDRIISPLKKSPLKQEFLEKNSSP 240
           VPGKSSSFLFLPEDGLGKENQSYANTVKNKPSPEKKVDRIISPLKKSPLKQEFLEKNSSP
Sbjct: 212 VPGKSSSFLFLPEDGLGKENQSYANTVKNKPSPEKKVDRIISPLKKSPLKQEFLEKNSSP 271

Query: 241 MKSQLEFRLERERAKDNSSNLSDDTEASSSPNKISEDIVKCLSSIFIRLSSSKDKATDSS 300
           MKSQLEFRLERERAKDNSSNLSDDTEASSSPNKISEDIVKCLSSIFIRLSSSKDKATDSS
Sbjct: 272 MKSQLEFRLERERAKDNSSNLSDDTEASSSPNKISEDIVKCLSSIFIRLSSSKDKATDSS 331

Query: 301 DTSSGPAELQDPYDACSDFKPRNIGPYRHLCAIEASSVDLDRSTNAVFLIHRLKNLFRRL 360
           DTSSGPAELQDPYDACSDFKPRNIGPYRHLCAIEASSVDLDRSTNAVFLIHRLKNLFRRL
Sbjct: 332 DTSSGPAELQDPYDACSDFKPRNIGPYRHLCAIEASSVDLDRSTNAVFLIHRLKNLFRRL 391

Query: 361 ASVNLAGLNHQEKLAFWINTYNSCMMNAFLEQGIPETHERVVTLMQKATIIVGGHLLNAI 420
           ASVNLAGLNHQEKLAFWINTYNSCMMNAFLEQGIPETHERVVTLMQKATIIVGGHLLNAI
Sbjct: 392 ASVNLAGLNHQEKLAFWINTYNSCMMNAFLEQGIPETHERVVTLMQKATIIVGGHLLNAI 451

Query: 421 TIEHFILRLPYHLKFTCPKAVKNDEMRARSVFGLEYSEPLITFALCCGSWSSPAVRVYSG 480
           TIEHFILRLPYHLKFTCPKAVKNDEMRARSVFGLEYSEPLITFALCCGSWSSPAVRVYSG
Sbjct: 452 TIEHFILRLPYHLKFTCPKAVKNDEMRARSVFGLEYSEPLITFALCCGSWSSPAVRVYSG 511

Query: 481 CKVEEELEVAKREYLQAAVGISKTNNKLMIPKVLDWYLLDFAKDLESMLDWICLQLPNEL 540
           CKVEEELEVAKREYLQAAVGISKTNNKLMIPKVLDWYLLDFAKDLESMLDWICLQLPNEL
Sbjct: 512 CKVEEELEVAKREYLQAAVGISKTNNKLMIPKVLDWYLLDFAKDLESMLDWICLQLPNEL 571

Query: 541 RIEAVKCLERKGREPLSQLVQVMPYNFSFRMLLHR 576
           RIEAVKCLERKGREPLSQLVQVMPYNFSFRMLLHR
Sbjct: 572 RIEAVKCLERKGREPLSQLVQVMPYNFSFRMLLHR 606

BLAST of Cucsa.102320.4 vs. TrEMBL
Match: B9HE62_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0007s11280g PE=4 SV=2)

HSP 1 Score: 754.2 bits (1946), Expect = 1.2e-214
Identity = 398/582 (68.38%), Postives = 474/582 (81.44%), Query Frame = 1

Query: 10  INRRKLNRERKIALLQDVDKLKKKLRHEENVHKALERAFTRPLGALPRLPPYLPPYILEL 69
           +N RK N+ERK+ALLQDVDKLKKKLRHEENVH+ALERAFTRPLGALPRLPPYLPPYILEL
Sbjct: 42  MNWRKANKERKLALLQDVDKLKKKLRHEENVHRALERAFTRPLGALPRLPPYLPPYILEL 101

Query: 70  LAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYVSSSSRNSDISTDTME--PISTRIAKHRR 129
           LAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYVSS  +N + S D ++  P +TR +KH R
Sbjct: 102 LAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYVSSK-KNVENSKDAIDQQPSTTR-SKHAR 161

Query: 130 TKSYCQNEFNSANSIARLQPSLARCSSSRKLLSNDTFFDRNGNGSNRFAN-GKHVPGKSS 189
           +KS   NE NSA   AR QPSLARC+SSR+L S D   +R+G  SNR +N GK+  GK +
Sbjct: 162 SKSLSLNETNSATFAARPQPSLARCTSSRRLFSTDPIIERSGQCSNRPSNRGKYASGKPN 221

Query: 190 SFLFLPEDGLGKENQSYANTVKNKPSPEKKVDRIISPLKKSPLKQEFLEKNSSPMKSQLE 249
           S   L +DG GKEN+S  N VK+K SP+K   +I +P+K++P K+E  EK+  P K QLE
Sbjct: 222 SSSSLVDDGRGKENRSCINYVKDKQSPDKMA-KITTPVKRTPNKRESEEKSLEPSKLQLE 281

Query: 250 FRL-ERERAKDNSSNLSDDT--EASSSPNKISEDIVKCLSSIFIRLSSSKDKATDSSDTS 309
            RL E+ERA++++S   +D   E + +PNK++EDIVKCLSSIF+R+S+ KDK  +    S
Sbjct: 282 CRLIEQERAQESTSAYMNDRICENNITPNKLTEDIVKCLSSIFLRMSTLKDKVVELGTFS 341

Query: 310 SGPA----------ELQDPYDACSDFKPRNIGPYRHLCAIEASSVDLDRSTNAVFLIHRL 369
           S             E++DPY   ++FK R+IG Y+HL AIEASS+DL+R+T+A+FL+ RL
Sbjct: 342 SRATLTSPEGDRGNEIRDPYGMSAEFKIRDIGSYKHLYAIEASSIDLNRTTSALFLLQRL 401

Query: 370 KNLFRRLASVNLAGLNHQEKLAFWINTYNSCMMNAFLEQGIPETHERVVTLMQKATIIVG 429
           + L  +LA+ NL GL HQ+KLAFWINTYNSCMMNA LE GIPET E VV LMQKATI VG
Sbjct: 402 RFLLGKLAAANLEGLTHQQKLAFWINTYNSCMMNAILEHGIPETPEMVVALMQKATITVG 461

Query: 430 GHLLNAITIEHFILRLPYHLKFTCPKAVKNDEMRARSVFGLEYSEPLITFALCCGSWSSP 489
           GHLLNAITIEHFILRLPYHLKFTCPKAVKNDEM+ARS+FG E+SEPL+TFALCCGSWSSP
Sbjct: 462 GHLLNAITIEHFILRLPYHLKFTCPKAVKNDEMKARSIFGFEWSEPLVTFALCCGSWSSP 521

Query: 490 AVRVYSGCKVEEELEVAKREYLQAAVGISKTNNKLMIPKVLDWYLLDFAKDLESMLDWIC 549
           AVRVY+  +VEEELEVAKR+YLQA VGIS+T NKL+IPK+LDWYLLDFAKD+ES+LDWIC
Sbjct: 522 AVRVYTASRVEEELEVAKRDYLQATVGISRT-NKLIIPKLLDWYLLDFAKDMESLLDWIC 581

Query: 550 LQLPNELRIEAVKCLERKGREPLSQLVQVMPYNFSFRMLLHR 576
           LQLPNELR EAVKCLER+GR+PLSQ+VQVMPY+FSFR+LLHR
Sbjct: 582 LQLPNELRNEAVKCLERRGRDPLSQIVQVMPYDFSFRLLLHR 619

BLAST of Cucsa.102320.4 vs. TrEMBL
Match: U5G8B7_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0007s11280g PE=4 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 1.2e-214
Identity = 398/582 (68.38%), Postives = 474/582 (81.44%), Query Frame = 1

Query: 10  INRRKLNRERKIALLQDVDKLKKKLRHEENVHKALERAFTRPLGALPRLPPYLPPYILEL 69
           +N RK N+ERK+ALLQDVDKLKKKLRHEENVH+ALERAFTRPLGALPRLPPYLPPYILEL
Sbjct: 41  MNWRKANKERKLALLQDVDKLKKKLRHEENVHRALERAFTRPLGALPRLPPYLPPYILEL 100

Query: 70  LAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYVSSSSRNSDISTDTME--PISTRIAKHRR 129
           LAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYVSS  +N + S D ++  P +TR +KH R
Sbjct: 101 LAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYVSSK-KNVENSKDAIDQQPSTTR-SKHAR 160

Query: 130 TKSYCQNEFNSANSIARLQPSLARCSSSRKLLSNDTFFDRNGNGSNRFAN-GKHVPGKSS 189
           +KS   NE NSA   AR QPSLARC+SSR+L S D   +R+G  SNR +N GK+  GK +
Sbjct: 161 SKSLSLNETNSATFAARPQPSLARCTSSRRLFSTDPIIERSGQCSNRPSNRGKYASGKPN 220

Query: 190 SFLFLPEDGLGKENQSYANTVKNKPSPEKKVDRIISPLKKSPLKQEFLEKNSSPMKSQLE 249
           S   L +DG GKEN+S  N VK+K SP+K   +I +P+K++P K+E  EK+  P K QLE
Sbjct: 221 SSSSLVDDGRGKENRSCINYVKDKQSPDKMA-KITTPVKRTPNKRESEEKSLEPSKLQLE 280

Query: 250 FRL-ERERAKDNSSNLSDDT--EASSSPNKISEDIVKCLSSIFIRLSSSKDKATDSSDTS 309
            RL E+ERA++++S   +D   E + +PNK++EDIVKCLSSIF+R+S+ KDK  +    S
Sbjct: 281 CRLIEQERAQESTSAYMNDRICENNITPNKLTEDIVKCLSSIFLRMSTLKDKVVELGTFS 340

Query: 310 SGPA----------ELQDPYDACSDFKPRNIGPYRHLCAIEASSVDLDRSTNAVFLIHRL 369
           S             E++DPY   ++FK R+IG Y+HL AIEASS+DL+R+T+A+FL+ RL
Sbjct: 341 SRATLTSPEGDRGNEIRDPYGMSAEFKIRDIGSYKHLYAIEASSIDLNRTTSALFLLQRL 400

Query: 370 KNLFRRLASVNLAGLNHQEKLAFWINTYNSCMMNAFLEQGIPETHERVVTLMQKATIIVG 429
           + L  +LA+ NL GL HQ+KLAFWINTYNSCMMNA LE GIPET E VV LMQKATI VG
Sbjct: 401 RFLLGKLAAANLEGLTHQQKLAFWINTYNSCMMNAILEHGIPETPEMVVALMQKATITVG 460

Query: 430 GHLLNAITIEHFILRLPYHLKFTCPKAVKNDEMRARSVFGLEYSEPLITFALCCGSWSSP 489
           GHLLNAITIEHFILRLPYHLKFTCPKAVKNDEM+ARS+FG E+SEPL+TFALCCGSWSSP
Sbjct: 461 GHLLNAITIEHFILRLPYHLKFTCPKAVKNDEMKARSIFGFEWSEPLVTFALCCGSWSSP 520

Query: 490 AVRVYSGCKVEEELEVAKREYLQAAVGISKTNNKLMIPKVLDWYLLDFAKDLESMLDWIC 549
           AVRVY+  +VEEELEVAKR+YLQA VGIS+T NKL+IPK+LDWYLLDFAKD+ES+LDWIC
Sbjct: 521 AVRVYTASRVEEELEVAKRDYLQATVGISRT-NKLIIPKLLDWYLLDFAKDMESLLDWIC 580

Query: 550 LQLPNELRIEAVKCLERKGREPLSQLVQVMPYNFSFRMLLHR 576
           LQLPNELR EAVKCLER+GR+PLSQ+VQVMPY+FSFR+LLHR
Sbjct: 581 LQLPNELRNEAVKCLERRGRDPLSQIVQVMPYDFSFRLLLHR 618

BLAST of Cucsa.102320.4 vs. TrEMBL
Match: A0A0D2TZ30_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_008G074600 PE=4 SV=1)

HSP 1 Score: 746.5 bits (1926), Expect = 2.4e-212
Identity = 384/570 (67.37%), Postives = 460/570 (80.70%), Query Frame = 1

Query: 9   IINRRKLNRERKIALLQDVDKLKKKLRHEENVHKALERAFTRPLGALPRLPPYLPPYILE 68
           + NRR+  RE+KIALLQDVDKLK+KLRHEENVH+ALERAFTRPLGALPRLPPYLPPY LE
Sbjct: 43  VTNRRRSIREKKIALLQDVDKLKRKLRHEENVHRALERAFTRPLGALPRLPPYLPPYTLE 102

Query: 69  LLAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYVSSSSRNSDISTDTMEPISTRIAKHRRT 128
           LLAEVAVLEEEVVRLEEQVV+FRQGLYQEAVY SS     +++  T+E    R +KH+R+
Sbjct: 103 LLAEVAVLEEEVVRLEEQVVSFRQGLYQEAVYASSKRNVENLNESTVEQSPVRSSKHQRS 162

Query: 129 KSYCQNEFNSANSIARLQPSLARCSSSRKLLSNDTFFDRNGNGSNRFANGKHVPGKSSSF 188
           KS   NE +   +IAR QPSL+R  SSRK+L  DT +DR G    R  NGK     S+ F
Sbjct: 163 KSLSVNEMSLVPTIARSQPSLSRSVSSRKMLPPDTIYDRAGQCFIRPTNGKQA---STKF 222

Query: 189 LFLPEDGLGKENQSYANTVKNKPSPEKKVDRIISPLKKSPLKQEFLEKNSSPMKSQLEFR 248
                D  GKENQS+AN VK+K SP+KKV  I++P+K+ P K +  +K   P+K QL+ R
Sbjct: 223 NSASGDVRGKENQSFANAVKDKQSPDKKVSNIVTPVKRLPTKLDSADKYLDPLKVQLDGR 282

Query: 249 L-ERERAKDNSSNLSDD--TEASSSPNKISEDIVKCLSSIFIRLSSSKDKATDSSDTSSG 308
           L ++E A+ + S+ +DD  +E  S+PNKISEDIV+CL  IF RLS+ KDKA +S  T   
Sbjct: 283 LVKQENAQASPSSSTDDKVSEVDSAPNKISEDIVRCLFRIFARLSTLKDKAAESG-TLPR 342

Query: 309 PAELQDPYDACSDFKPRNIGPYRHLCAIEASSVDLDRSTNAVFLIHRLKNLFRRLASVNL 368
            +E++DPY  CSD K R+IGPY+HLCAIE ++++L+RSTNA+FLIH+LK L  +LASVNL
Sbjct: 343 ESEVRDPYGICSDLKTRDIGPYQHLCAIEVNTINLNRSTNALFLIHKLKFLLGKLASVNL 402

Query: 369 AGLNHQEKLAFWINTYNSCMMNAFLEQGIPETHERVVTLMQKATIIVGGHLLNAITIEHF 428
            GL+HQ+KLAFWINTYNSCMMNA LE G+PE  ERVV LMQKATI+VGGHLLNAITIEHF
Sbjct: 403 EGLSHQQKLAFWINTYNSCMMNAILEHGVPELPERVVALMQKATIVVGGHLLNAITIEHF 462

Query: 429 ILRLPYHLKFTCPKAVKNDEMRARSVFGLEYSEPLITFALCCGSWSSPAVRVYSGCKVEE 488
           ILRLP+HLKFTCPKA KNDE++AR++FGLE+SEPL+TFAL CGSWSSPAVRVY+   VEE
Sbjct: 463 ILRLPFHLKFTCPKATKNDEVKARNIFGLEWSEPLVTFALACGSWSSPAVRVYTAAHVEE 522

Query: 489 ELEVAKREYLQAAVGISKTNNKLMIPKVLDWYLLDFAKDLESMLDWICLQLPNELRIEAV 548
           ELE AKR+YLQAAVGIS+T NKL IPK+LDWYLLDFAKDLES+LDW+CLQLPN++R EAV
Sbjct: 523 ELETAKRDYLQAAVGISRT-NKLTIPKLLDWYLLDFAKDLESLLDWVCLQLPNDVRNEAV 582

Query: 549 KCLERKGREPLSQLVQVMPYNFSFRMLLHR 576
           KCLER G+E LSQLVQVMPY+FSFR+LL R
Sbjct: 583 KCLERNGKETLSQLVQVMPYDFSFRLLLQR 607

BLAST of Cucsa.102320.4 vs. TrEMBL
Match: A0A0D2RBE6_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_008G074600 PE=4 SV=1)

HSP 1 Score: 746.5 bits (1926), Expect = 2.4e-212
Identity = 384/570 (67.37%), Postives = 460/570 (80.70%), Query Frame = 1

Query: 9   IINRRKLNRERKIALLQDVDKLKKKLRHEENVHKALERAFTRPLGALPRLPPYLPPYILE 68
           + NRR+  RE+KIALLQDVDKLK+KLRHEENVH+ALERAFTRPLGALPRLPPYLPPY LE
Sbjct: 16  VTNRRRSIREKKIALLQDVDKLKRKLRHEENVHRALERAFTRPLGALPRLPPYLPPYTLE 75

Query: 69  LLAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYVSSSSRNSDISTDTMEPISTRIAKHRRT 128
           LLAEVAVLEEEVVRLEEQVV+FRQGLYQEAVY SS     +++  T+E    R +KH+R+
Sbjct: 76  LLAEVAVLEEEVVRLEEQVVSFRQGLYQEAVYASSKRNVENLNESTVEQSPVRSSKHQRS 135

Query: 129 KSYCQNEFNSANSIARLQPSLARCSSSRKLLSNDTFFDRNGNGSNRFANGKHVPGKSSSF 188
           KS   NE +   +IAR QPSL+R  SSRK+L  DT +DR G    R  NGK     S+ F
Sbjct: 136 KSLSVNEMSLVPTIARSQPSLSRSVSSRKMLPPDTIYDRAGQCFIRPTNGKQA---STKF 195

Query: 189 LFLPEDGLGKENQSYANTVKNKPSPEKKVDRIISPLKKSPLKQEFLEKNSSPMKSQLEFR 248
                D  GKENQS+AN VK+K SP+KKV  I++P+K+ P K +  +K   P+K QL+ R
Sbjct: 196 NSASGDVRGKENQSFANAVKDKQSPDKKVSNIVTPVKRLPTKLDSADKYLDPLKVQLDGR 255

Query: 249 L-ERERAKDNSSNLSDD--TEASSSPNKISEDIVKCLSSIFIRLSSSKDKATDSSDTSSG 308
           L ++E A+ + S+ +DD  +E  S+PNKISEDIV+CL  IF RLS+ KDKA +S  T   
Sbjct: 256 LVKQENAQASPSSSTDDKVSEVDSAPNKISEDIVRCLFRIFARLSTLKDKAAESG-TLPR 315

Query: 309 PAELQDPYDACSDFKPRNIGPYRHLCAIEASSVDLDRSTNAVFLIHRLKNLFRRLASVNL 368
            +E++DPY  CSD K R+IGPY+HLCAIE ++++L+RSTNA+FLIH+LK L  +LASVNL
Sbjct: 316 ESEVRDPYGICSDLKTRDIGPYQHLCAIEVNTINLNRSTNALFLIHKLKFLLGKLASVNL 375

Query: 369 AGLNHQEKLAFWINTYNSCMMNAFLEQGIPETHERVVTLMQKATIIVGGHLLNAITIEHF 428
            GL+HQ+KLAFWINTYNSCMMNA LE G+PE  ERVV LMQKATI+VGGHLLNAITIEHF
Sbjct: 376 EGLSHQQKLAFWINTYNSCMMNAILEHGVPELPERVVALMQKATIVVGGHLLNAITIEHF 435

Query: 429 ILRLPYHLKFTCPKAVKNDEMRARSVFGLEYSEPLITFALCCGSWSSPAVRVYSGCKVEE 488
           ILRLP+HLKFTCPKA KNDE++AR++FGLE+SEPL+TFAL CGSWSSPAVRVY+   VEE
Sbjct: 436 ILRLPFHLKFTCPKATKNDEVKARNIFGLEWSEPLVTFALACGSWSSPAVRVYTAAHVEE 495

Query: 489 ELEVAKREYLQAAVGISKTNNKLMIPKVLDWYLLDFAKDLESMLDWICLQLPNELRIEAV 548
           ELE AKR+YLQAAVGIS+T NKL IPK+LDWYLLDFAKDLES+LDW+CLQLPN++R EAV
Sbjct: 496 ELETAKRDYLQAAVGISRT-NKLTIPKLLDWYLLDFAKDLESLLDWVCLQLPNDVRNEAV 555

Query: 549 KCLERKGREPLSQLVQVMPYNFSFRMLLHR 576
           KCLER G+E LSQLVQVMPY+FSFR+LL R
Sbjct: 556 KCLERNGKETLSQLVQVMPYDFSFRLLLQR 580

BLAST of Cucsa.102320.4 vs. TAIR10
Match: AT4G37080.2 (AT4G37080.2 Protein of unknown function, DUF547)

HSP 1 Score: 644.0 bits (1660), Expect = 8.5e-185
Identity = 351/584 (60.10%), Postives = 434/584 (74.32%), Query Frame = 1

Query: 8   TIINRRKLNRERKIALLQDVDKLKKKLRHEENVHKALERAFTRPLGALPRLPPYLPPYIL 67
           +++NRR+ N+E+K+ LLQDVDKLK+KLR EENVH+ALERAFTRPLGALPRLP YLP + L
Sbjct: 44  SLVNRRRANKEKKMDLLQDVDKLKRKLRQEENVHRALERAFTRPLGALPRLPSYLPRHTL 103

Query: 68  ELLAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYVSSSSRNSDISTDTMEPIS-TRIAKHR 127
           ELLAEVAVLEEEVVRLEEQVVNFRQGLYQEAVY+SS       + +++   S  R  KH+
Sbjct: 104 ELLAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYISSKRNLESPNNNSLNENSPVRSTKHQ 163

Query: 128 RTKSYCQNEFNSA-NSIARLQPSLARCSSSRKLLSND-TFFDRNGNGSNRFANGKHVPGK 187
           R+KS  Q+EFNS      + Q SL+R  SSRKL S+D T  DR+G    R  +GK    K
Sbjct: 164 RSKSMSQHEFNSMITPTKKHQQSLSRSISSRKLFSSDQTVNDRSGQ---RVVSGKQASPK 223

Query: 188 SSSFLFL---PEDGLGKENQSYANTVK---NKPSPEKKVDRIISPLKKS-PL--KQEFLE 247
           S+        P D  GKENQ+ +N  K   NK SPEKK+ R ++ +KK  PL   +   +
Sbjct: 224 SNLSSVTNTKPVDVRGKENQTSSNASKDKKNKESPEKKLGRFLTSVKKKKPLIKPEAAAD 283

Query: 248 KNSSPMKSQLEFRL-ERERAKDNSSNLSDDTEASSS---PNKISEDIVKCLSSIFIRLSS 307
           K+S   K QL+ RL ++++A+++ S  S + +   S    N++SED++KCL +I +R+SS
Sbjct: 284 KHSESTKLQLDDRLADQDKAQESVSGSSSEDKTLQSGNVANRVSEDLLKCLVTIILRISS 343

Query: 308 SKDKATDSSDTSSGPAELQDPYDACSDFKPRNIGPYRHLCAIEASSVDLDRSTNAVFLIH 367
           SKD   D             PY+ CS+++ R +G Y+H  +++ SSVDL R  NA FLIH
Sbjct: 344 SKDIVLD-------------PYNNCSEWRTRELGAYKHFSSVDTSSVDLGRRINASFLIH 403

Query: 368 RLKNLFRRLASVNLAGLNHQEKLAFWINTYNSCMMNAFLEQGIPETHERVVTLMQKATII 427
           RLK L  +L+ VNL GL+HQ+KLAFWINTYNSC+MNAFLE GIP T E VV LMQKATII
Sbjct: 404 RLKFLLNKLSVVNLDGLSHQQKLAFWINTYNSCVMNAFLEHGIPATPEMVVALMQKATII 463

Query: 428 VGGHLLNAITIEHFILRLPYHLKFTCPKAVKNDEMRARSVFGLEYSEPLITFALCCGSWS 487
           VGGH LNAITIEHFILRLPYHLKFTCPK   ++EMRA S FGLE+SEPL+TFAL CGSWS
Sbjct: 464 VGGHSLNAITIEHFILRLPYHLKFTCPKTATHEEMRAHSTFGLEWSEPLVTFALACGSWS 523

Query: 488 SPAVRVYSGCKVEEELEVAKREYLQAAVGISKTNNKLMIPKVLDWYLLDFAKDLESMLDW 547
           SPAVRVY+   VEEELE AKR+YLQA+VGISK NNKLM+PKVLDWYLLDFAKDLES+LDW
Sbjct: 524 SPAVRVYTAANVEEELEAAKRDYLQASVGISK-NNKLMLPKVLDWYLLDFAKDLESLLDW 583

Query: 548 ICLQLPNELRIEAVKCLERKGREPLSQLVQVMPYNFSFRMLLHR 576
           +CLQLP++LR EA KC+ERK +E L +LVQV+PY+FSFR+LLH+
Sbjct: 584 VCLQLPDKLREEATKCMERKNKESLMELVQVVPYDFSFRLLLHQ 610

BLAST of Cucsa.102320.4 vs. TAIR10
Match: AT5G42690.2 (AT5G42690.2 Protein of unknown function, DUF547)

HSP 1 Score: 482.3 bits (1240), Expect = 4.3e-136
Identity = 285/567 (50.26%), Postives = 363/567 (64.02%), Query Frame = 1

Query: 9   IINRRKLNRERKIALLQDVDKLKKKLRHEENVHKALERAFTRPLGALPRLPPYLPPYILE 68
           ++NR+ LNRE+ I L +DV+KL+KKLR EEN+H+A+ERAF+RPLGALPRLPP+LPP +LE
Sbjct: 41  VVNRKALNREKIITLQEDVEKLRKKLRLEENIHRAMERAFSRPLGALPRLPPFLPPSVLE 100

Query: 69  LLAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYVSSSSRNSDISTDTMEPISTRIAKHRRT 128
           LLAEVAVLEEE+VRLEE +V+ RQ LYQEAV+ SSS  N           S    KH +T
Sbjct: 101 LLAEVAVLEEELVRLEEHIVHCRQELYQEAVFTSSSIENL--------KCSPAFPKHWQT 160

Query: 129 KSYCQNEFNSANSIARLQPS-LARCSSSRKLLSNDTFFDRNGNGSNRFANGKHVPGKSSS 188
           KS       SA++ AR   S L+R   S  +        R G  +   A     P K ++
Sbjct: 161 KS------KSASTSARESESPLSRAPCSVSVC-------RKGKENKLSATSIKTPMKKTT 220

Query: 189 FLFLPEDGLGKENQSYANTVKNKPSPEKKVDRIISPLKKSPLKQEFLEKNSSPMKSQLEF 248
                           A+T  NK             L+   LKQ+               
Sbjct: 221 I---------------AHTQLNK------------SLEAQKLKQD-------------SH 280

Query: 249 RLERERAKDNSSNLSDDTEASSSPNKISEDIVKCLSSIFIRLSSSKDKATDSSDTSSGPA 308
           R  +  A+ +S    D+      PNKISED+VKCLS+IF+R+SS K      S  +    
Sbjct: 281 RCRKTNAERSSHGGGDE------PNKISEDLVKCLSNIFMRMSSIKRSMVTKSQENDKDT 340

Query: 309 ELQDPYDACSDFKPRNIGPYRHLCAIEASSVDLDR-STNAVFLIHRLKNLFRRLASVNLA 368
             +DPY  CS F+ R+IG Y++   +E +S++ +R S++++FLI +LK L  RL+ VN+ 
Sbjct: 341 AFRDPYGICSSFRRRDIGRYKNFSDVEEASLNQNRTSSSSLFLIRQLKRLLGRLSLVNMQ 400

Query: 369 GLNHQEKLAFWINTYNSCMMNAFLEQGIPETHERVVTLMQKATIIVGGHLLNAITIEHFI 428
            LN QEKLAFWIN YNSCMMN FLE GIPE+ + +VTLMQKATI VGGH LNAITIEHFI
Sbjct: 401 KLNQQEKLAFWINIYNSCMMNGFLEHGIPESPD-MVTLMQKATINVGGHFLNAITIEHFI 460

Query: 429 LRLPYHLKFTCPKAVKNDEMRARSVFGLEYSEPLITFALCCGSWSSPAVRVYSGCKVEEE 488
           LRLP+H K+  PK  K +EM  RS FGLE SEPL+TFAL CGSWSSPAVRVY+  KVEEE
Sbjct: 461 LRLPHHSKYISPKGSKKNEMAVRSKFGLELSEPLVTFALSCGSWSSPAVRVYTASKVEEE 520

Query: 489 LEVAKREYLQAAVGISKTNNKLMIPKVLDWYLLDFAKDLESMLDWICLQLPNELRIEAVK 548
           LEVAKREYL+A+VGIS    K+ IPK++DWY  DFAKD+ES+LDWI LQLP EL  +A+ 
Sbjct: 521 LEVAKREYLEASVGISVV--KIGIPKLMDWYSHDFAKDIESLLDWIFLQLPTELGKDALN 537

Query: 549 CLER-KGREPLSQLVQVMPYNFSFRML 573
           C+E+   + P S LV ++PY+F+FR L
Sbjct: 581 CVEQGMSQSPSSTLVHIIPYDFTFRYL 537

BLAST of Cucsa.102320.4 vs. TAIR10
Match: AT2G39690.1 (AT2G39690.1 Protein of unknown function, DUF547)

HSP 1 Score: 244.2 bits (622), Expect = 2.0e-64
Identity = 131/331 (39.58%), Postives = 201/331 (60.73%), Query Frame = 1

Query: 264 DTEASSSPNKISEDIVKCLSSIFIRLSSSKDKATDSSDTSSGPAELQ------------- 323
           + +    PN++SE ++ CL  I++ L+    K       S  P+                
Sbjct: 170 EAKTKDDPNEVSEQLINCLIGIYLELNHVSSKTKGDVSLSRRPSSCSRKSNTYSYYQNAM 229

Query: 324 --DPYDACSDFK---PRNIGPYRHLCAIEASSVDLDRSTNAVF-LIHRLKNLFRRLASVN 383
             DPY    D      R+IGPY++   I  SS+D+   T+     + RL  L  +L+ V+
Sbjct: 230 NLDPYHVLPDSSGGVTRDIGPYKNFIHISRSSIDVTHFTHYCSPAVPRLSVLMEKLSEVD 289

Query: 384 LAGLNHQEKLAFWINTYNSCMMNAFLEQGIPETHERVVTLMQKATIIVGGHLLNAITIEH 443
           L+ L +++KLAFWIN YN+C+M+AFLE G+P +H R++TLM KA++ VGG +LNA+ IEH
Sbjct: 290 LSFLTYKQKLAFWINIYNACIMHAFLEYGLPSSHNRLLTLMNKASLNVGGIVLNALAIEH 349

Query: 444 FILRLPYHLKFTCPKAVKNDEMRARSVFGLEYSEPLITFALCCGSWSSPAVRVYSGCKVE 503
           F+LR P   +     ++   E   R  +GL YSEP +TFALC GSWSSPA+RVY+  +V 
Sbjct: 350 FVLRHPCEPE---DDSLDEKETLLRHTYGLGYSEPNVTFALCRGSWSSPALRVYTADEVV 409

Query: 504 EELEVAKREYLQAAVGISKTNNKLMIPKVLDWYLLDFAKDLESMLDWICLQLP--NELRI 563
            +L  A+ EYL+A+VG+S +  K+++P++L W++ DFA D+ES+L+WI  QLP    L+ 
Sbjct: 410 NDLGRARVEYLEASVGVS-SKKKIVVPQLLQWHMKDFADDIESLLEWIYSQLPRSGNLKG 469

Query: 564 EAVKCLERKGREPLSQLVQVMPYNFSFRMLL 574
             ++CL+RK + PL+++V++  Y   FR LL
Sbjct: 470 MIMECLKRKAKVPLAKIVEIQTYGHEFRYLL 496


HSP 2 Score: 218.4 bits (555), Expect = 1.2e-56
Identity = 169/555 (30.45%), Postives = 272/555 (49.01%), Query Frame = 1

Query: 30  LKKKLRHEENVHKALERAFTRPLGALPRLPP-YLPPYILELLAEVAVLEEEVVRLEEQVV 89
           LK++L  EE V + L  AF   + +LP L   +LPP   EL+ E+A++E E++ L+ ++ 
Sbjct: 3   LKERLEEEEAVTRTLRVAFDGSIVSLPSLSSLFLPPQFSELIQELAIVEAEILCLDRKIE 62

Query: 90  NFRQGLYQEAVYVSSSSRNSDISTDTMEPISTRIAKHRRTKSYCQNEFNSANSIAR--LQ 149
             +  LY E                T E I  ++ + +RT +   +   S   +     Q
Sbjct: 63  ELKLKLYSE-------------QRQTQE-IQLQMTEQKRTLARQSHVRQSTLPLRHDLHQ 122

Query: 150 PSLARCSSSRKLLSNDTFFDR-NGNGSNRFANGKHVPGKSSSFLFLPEDGLGKENQSYAN 209
            SL+ C     L +  T   R + + +  F +     G +  F  +    +G+  +    
Sbjct: 123 RSLSHCYQRSTLDTASTTHSRLSFSYAPDFLDATSSGGFTDEFDGVTRMQMGRVRKGLRL 182

Query: 210 T-VKNKPSPEKKVDRIISPLKKSPLKQEFLEKNSSPMKSQLEFRLERERAKDNSSNLSDD 269
              K K  P +  +++I+ L        +LE N                    SS    D
Sbjct: 183 VEAKTKDDPNEVSEQLINCLIGI-----YLELNHV------------------SSKTKGD 242

Query: 270 TEASSSPNKISEDIVKCLSSIFIRLSSSKDKATDSSDTSSGPAELQDPYDACSDFKPRNI 329
              S  P+  S    K  +  + + + + D      D+S G      PY         +I
Sbjct: 243 VSLSRRPSSCSR---KSNTYSYYQNAMNLDPYHVLPDSSGGVTRDIGPYKNFIHISRSSI 302

Query: 330 G--PYRHLC--AIEASSVDLDRSTNAVFLIHRLKNLFRRLASVNLAGLNHQEKLAFWINT 389
               + H C  A+   SV +++ +                       L +++KLAFWIN 
Sbjct: 303 DVTHFTHYCSPAVPRLSVLMEKLSEVDLSF-----------------LTYKQKLAFWINI 362

Query: 390 YNSCMMNAFLEQGIPETHERVVTLMQKATIIVGGHLLNAITIEHFILRLPYHLKFTCPKA 449
           YN+C+M+AFLE G+P +H R++TLM KA++ VGG +LNA+ IEHF+LR P   +     +
Sbjct: 363 YNACIMHAFLEYGLPSSHNRLLTLMNKASLNVGGIVLNALAIEHFVLRHPCEPE---DDS 422

Query: 450 VKNDEMRARSVFGLEYSEPLITFALCCGSWSSPAVRVYSGCKVEEELEVAKREYLQAAVG 509
           +   E   R  +GL YSEP +TFALC GSWSSPA+RVY+  +V  +L  A+ EYL+A+VG
Sbjct: 423 LDEKETLLRHTYGLGYSEPNVTFALCRGSWSSPALRVYTADEVVNDLGRARVEYLEASVG 482

Query: 510 ISKTNNKLMIPKVLDWYLLDFAKDLESMLDWICLQLP--NELRIEAVKCLERKGREPLSQ 569
           +S +  K+++P++L W++ DFA D+ES+L+WI  QLP    L+   ++CL+RK + PL++
Sbjct: 483 VS-SKKKIVVPQLLQWHMKDFADDIESLLEWIYSQLPRSGNLKGMIMECLKRKAKVPLAK 496

Query: 570 LVQVMPYNFSFRMLL 574
           +V++  Y   FR LL
Sbjct: 543 IVEIQTYGHEFRYLL 496

BLAST of Cucsa.102320.4 vs. TAIR10
Match: AT3G12540.1 (AT3G12540.1 Protein of unknown function, DUF547)

HSP 1 Score: 212.2 bits (539), Expect = 8.3e-55
Identity = 127/357 (35.57%), Postives = 200/357 (56.02%), Query Frame = 1

Query: 243 SQLEF--RLERERAKDNSSNLSDDTEAS-SSPNKISEDIVKCLSSIFIRLSSSKDKATDS 302
           S +EF  R+      D  S   +      ++PN +SED+VKCL  I++ L+ S  +   S
Sbjct: 150 STMEFSSRIHSSTFSDGMSRTQEKNNVQETTPNGVSEDLVKCLMGIYLELNRSSREREGS 209

Query: 303 SDTSS------------------GPAELQDPYDACSDFKPRNIGPYRHLCAIEASSVDLD 362
              S                     A   DPY A      R+IG Y++   I  +S+D+ 
Sbjct: 210 KTVSKLSLTHLKNASFKRKSVYDHNASNLDPYGAVMGTSLRDIGEYKNFIHITRTSIDVS 269

Query: 363 RSTNAVFLIHRLKNLFRRLASVNLAGLNHQEKLAFWINTYNSCMMNAFLEQGIPETHERV 422
           R ++    +  L+ L  +L+ V+L+ LNH++K+AFWINTYN+C+MN FLE G+P + E++
Sbjct: 270 RLSDCSTSLVNLRVLKEKLSKVDLSFLNHKKKMAFWINTYNACVMNGFLEHGLPSSKEKL 329

Query: 423 VTLMQKATIIVGGHLLNAITIEHFILRLPYHLKFTCPKAVKNDEMRARSVFGLEYSEPLI 482
           +T+++ ATI VGG  L+A+ IE  IL+ P   + +   +    E+R ++ +G    EP +
Sbjct: 330 LTILKMATIDVGGTQLSALDIEGSILQSPCEPRESV--SAGESEVRIQTRYGFRCVEPNL 389

Query: 483 TFALCCGSWSSPAVRVYSGCKVEEELEVAKREYLQAAVGISKTNNKLMIPKVLDWYLLDF 542
            F LC G WSSPA+RVY+   V  EL  A+ EYL+A++G+S    K++IP+ L   L DF
Sbjct: 390 MFVLCRGDWSSPALRVYTAEDVVNELIKARTEYLEASIGVS-GRKKIVIPRFLHKRLRDF 449

Query: 543 AKDLESMLDWICLQLPN-----ELRIEAVKCLERKGREPLSQLVQVMPYNFSFRMLL 574
           A+D  S+++WIC QLP      +L+  A++ L +K    L +L++V  + + FR LL
Sbjct: 450 AEDEGSLIEWICSQLPPAQRCFQLKETAMEGLNKKSESQLKKLIEVRSHEYEFRYLL 503


HSP 2 Score: 43.1 bits (100), Expect = 6.7e-04
Identity = 23/70 (32.86%), Postives = 43/70 (61.43%), Query Frame = 1

Query: 29 KLKKKLRHEENVHKALERAFTRPLGALPRLPPYL-PPYILELLAEVAVLEEEVVRLEEQV 88
          K++ +L  E+ ++KAL      P+ + PRL   L PP + EL+ E+A +E E++ LE+++
Sbjct: 2  KMQGELEDEQALNKALRDMHRGPVMSQPRLSLLLLPPQVQELIEELATVEAEILCLEKRI 61

Query: 89 VNFRQGLYQE 98
           + +  +Y E
Sbjct: 62 QDLKLDVYSE 71

BLAST of Cucsa.102320.4 vs. TAIR10
Match: AT5G60720.1 (AT5G60720.1 Protein of unknown function, DUF547)

HSP 1 Score: 177.9 bits (450), Expect = 1.7e-44
Identity = 117/313 (37.38%), Postives = 183/313 (58.47%), Query Frame = 1

Query: 282 LSSIFIRLSSSKDKATDSSD-TSSGPAELQDPYDA--CSDFKPRNIGPYRHLCAIEASSV 341
           LSS   R+ ++    + S +  S   +  QDPY          R+IGPY++L    +SS+
Sbjct: 381 LSSRSFRVDNATSSLSKSMNLVSYKESRQQDPYGIFDVESSLARDIGPYKNLVIFTSSSM 440

Query: 342 DLD--RSTNAVFLIHRLKNLFRRLASVNLAGLNHQEKLAFWINTYNSCMMNAFLEQGIPE 401
           D     S+++V LI +L+ L   L +V+L  L+HQ+KLAFWIN +N+C+M+ +L+ G+P+
Sbjct: 441 DSKCISSSSSVSLIQKLRVLMNNLETVDLKVLSHQQKLAFWINMFNACVMHGYLQHGVPK 500

Query: 402 THERVVTLM-QKATIIVGGHLLNAITIEHFILRLPYHLKFTCPKAVKNDEMRARSVFGLE 461
           T ER+ +L+  KAT+ VGG  ++A TIEH ILR       T     +++EM  R ++G+E
Sbjct: 501 TAERLQSLVYNKATMNVGGKNISAHTIEHCILRKSTSSTMT---QDRHEEMIIRKLYGVE 560

Query: 462 YSEPLITFALCCGSWSSPAVRVYSGCKVEEELEVAKREYLQAAVGISKTNNKLMIPKVLD 521
            ++P ITFAL CG+ SSPAVR+Y+G  V  ELE +K EYLQA++ ++    ++ +P++L 
Sbjct: 561 ATDPNITFALSCGTRSSPAVRIYTGEGVTTELEKSKLEYLQASLVVTAA-KRIGLPELLL 620

Query: 522 WYLLDFA-----------KDLESMLDWICLQLP--NELRIEAVKCLERKGRE--PLSQLV 574
            +  DF            + L S++ W+C QLP    LR   V C +    +    S  V
Sbjct: 621 KHATDFVVLTADGGTGEMEQLGSLVKWVCNQLPTSGSLRKSMVDCFKNPNSKASSSSSAV 680


HSP 2 Score: 57.4 bits (137), Expect = 3.4e-08
Identity = 71/303 (23.43%), Postives = 140/303 (46.20%), Query Frame = 1

Query: 6   PGTIINRRKLNRERKIALLQDVDKLKKKLRHEENVHKALERAFTRPLGALPR---LPPYL 65
           P ++  ++   +++K  + ++V  L++ L  EE   + LE+     L +      LP  L
Sbjct: 62  PHSVRTKKPNGQQKKEEIEKEVWMLREMLDQEEKTREILEQVQKHQLPSSSSSLTLPASL 121

Query: 66  PPYILELLAEVAVLEEEVVRLEEQVVNFRQGLYQEA-----VYVSSSSRNSDISTDTMEP 125
           PP + EL+ E++++E E+ RLE Q+ + +  L QE         +SSSR +  +++T + 
Sbjct: 122 PPKMKELITELSIVEGEISRLEIQISHLQINLKQEQDETLKQATTSSSRRAWQTSETYKD 181

Query: 126 ISTRIAKHRRTKSYCQNEFNSA---NSIARLQPSLARCSSSRKLLSNDTFFDRNGNGSNR 185
            +    +      Y +    S      + + + +  + ++S     N TF  +  +  N+
Sbjct: 182 DNINPYQAPTLPKYPKLPPPSPMVNKGMMKSENNNTKSTTSHHQ-ENATFGTKTLHFINK 241

Query: 186 FANGKHVPG---KSSSFLFLPEDGLGKENQSYANTVKNKPSPEKKVDRIISPLKKSPLKQ 245
              G +      KS+  + + E    KEN       +NK     K+ +I +    SPL++
Sbjct: 242 AIKGDYAIESFRKSNEKVGVVE----KENHRSVQH-ENKVQENMKMKKIRTMKSPSPLRE 301

Query: 246 EFLEKNSSPMKSQ---LEFRLERERAKDNSSNLSDDTEASSS--PNKISEDIVKCLSSIF 290
                 + P K +   L+  L+      +S+ L +D +      PNK++E+I+KCL+ I+
Sbjct: 302 PRYTSPNKPNKDRVAALDASLDIPPKSLSSTILMEDGQNIQKWHPNKLAENIMKCLNFIY 358

BLAST of Cucsa.102320.4 vs. NCBI nr
Match: gi|778718344|ref|XP_011657851.1| (PREDICTED: uncharacterized protein LOC101217980 isoform X1 [Cucumis sativus])

HSP 1 Score: 1143.6 bits (2957), Expect = 0.0e+00
Identity = 575/575 (100.00%), Postives = 575/575 (100.00%), Query Frame = 1

Query: 1   MGNDKPGTIINRRKLNRERKIALLQDVDKLKKKLRHEENVHKALERAFTRPLGALPRLPP 60
           MGNDKPGTIINRRKLNRERKIALLQDVDKLKKKLRHEENVHKALERAFTRPLGALPRLPP
Sbjct: 32  MGNDKPGTIINRRKLNRERKIALLQDVDKLKKKLRHEENVHKALERAFTRPLGALPRLPP 91

Query: 61  YLPPYILELLAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYVSSSSRNSDISTDTMEPIST 120
           YLPPYILELLAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYVSSSSRNSDISTDTMEPIST
Sbjct: 92  YLPPYILELLAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYVSSSSRNSDISTDTMEPIST 151

Query: 121 RIAKHRRTKSYCQNEFNSANSIARLQPSLARCSSSRKLLSNDTFFDRNGNGSNRFANGKH 180
           RIAKHRRTKSYCQNEFNSANSIARLQPSLARCSSSRKLLSNDTFFDRNGNGSNRFANGKH
Sbjct: 152 RIAKHRRTKSYCQNEFNSANSIARLQPSLARCSSSRKLLSNDTFFDRNGNGSNRFANGKH 211

Query: 181 VPGKSSSFLFLPEDGLGKENQSYANTVKNKPSPEKKVDRIISPLKKSPLKQEFLEKNSSP 240
           VPGKSSSFLFLPEDGLGKENQSYANTVKNKPSPEKKVDRIISPLKKSPLKQEFLEKNSSP
Sbjct: 212 VPGKSSSFLFLPEDGLGKENQSYANTVKNKPSPEKKVDRIISPLKKSPLKQEFLEKNSSP 271

Query: 241 MKSQLEFRLERERAKDNSSNLSDDTEASSSPNKISEDIVKCLSSIFIRLSSSKDKATDSS 300
           MKSQLEFRLERERAKDNSSNLSDDTEASSSPNKISEDIVKCLSSIFIRLSSSKDKATDSS
Sbjct: 272 MKSQLEFRLERERAKDNSSNLSDDTEASSSPNKISEDIVKCLSSIFIRLSSSKDKATDSS 331

Query: 301 DTSSGPAELQDPYDACSDFKPRNIGPYRHLCAIEASSVDLDRSTNAVFLIHRLKNLFRRL 360
           DTSSGPAELQDPYDACSDFKPRNIGPYRHLCAIEASSVDLDRSTNAVFLIHRLKNLFRRL
Sbjct: 332 DTSSGPAELQDPYDACSDFKPRNIGPYRHLCAIEASSVDLDRSTNAVFLIHRLKNLFRRL 391

Query: 361 ASVNLAGLNHQEKLAFWINTYNSCMMNAFLEQGIPETHERVVTLMQKATIIVGGHLLNAI 420
           ASVNLAGLNHQEKLAFWINTYNSCMMNAFLEQGIPETHERVVTLMQKATIIVGGHLLNAI
Sbjct: 392 ASVNLAGLNHQEKLAFWINTYNSCMMNAFLEQGIPETHERVVTLMQKATIIVGGHLLNAI 451

Query: 421 TIEHFILRLPYHLKFTCPKAVKNDEMRARSVFGLEYSEPLITFALCCGSWSSPAVRVYSG 480
           TIEHFILRLPYHLKFTCPKAVKNDEMRARSVFGLEYSEPLITFALCCGSWSSPAVRVYSG
Sbjct: 452 TIEHFILRLPYHLKFTCPKAVKNDEMRARSVFGLEYSEPLITFALCCGSWSSPAVRVYSG 511

Query: 481 CKVEEELEVAKREYLQAAVGISKTNNKLMIPKVLDWYLLDFAKDLESMLDWICLQLPNEL 540
           CKVEEELEVAKREYLQAAVGISKTNNKLMIPKVLDWYLLDFAKDLESMLDWICLQLPNEL
Sbjct: 512 CKVEEELEVAKREYLQAAVGISKTNNKLMIPKVLDWYLLDFAKDLESMLDWICLQLPNEL 571

Query: 541 RIEAVKCLERKGREPLSQLVQVMPYNFSFRMLLHR 576
           RIEAVKCLERKGREPLSQLVQVMPYNFSFRMLLHR
Sbjct: 572 RIEAVKCLERKGREPLSQLVQVMPYNFSFRMLLHR 606

BLAST of Cucsa.102320.4 vs. NCBI nr
Match: gi|778718350|ref|XP_011657854.1| (PREDICTED: uncharacterized protein LOC101217980 isoform X3 [Cucumis sativus])

HSP 1 Score: 1143.6 bits (2957), Expect = 0.0e+00
Identity = 575/575 (100.00%), Postives = 575/575 (100.00%), Query Frame = 1

Query: 1   MGNDKPGTIINRRKLNRERKIALLQDVDKLKKKLRHEENVHKALERAFTRPLGALPRLPP 60
           MGNDKPGTIINRRKLNRERKIALLQDVDKLKKKLRHEENVHKALERAFTRPLGALPRLPP
Sbjct: 16  MGNDKPGTIINRRKLNRERKIALLQDVDKLKKKLRHEENVHKALERAFTRPLGALPRLPP 75

Query: 61  YLPPYILELLAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYVSSSSRNSDISTDTMEPIST 120
           YLPPYILELLAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYVSSSSRNSDISTDTMEPIST
Sbjct: 76  YLPPYILELLAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYVSSSSRNSDISTDTMEPIST 135

Query: 121 RIAKHRRTKSYCQNEFNSANSIARLQPSLARCSSSRKLLSNDTFFDRNGNGSNRFANGKH 180
           RIAKHRRTKSYCQNEFNSANSIARLQPSLARCSSSRKLLSNDTFFDRNGNGSNRFANGKH
Sbjct: 136 RIAKHRRTKSYCQNEFNSANSIARLQPSLARCSSSRKLLSNDTFFDRNGNGSNRFANGKH 195

Query: 181 VPGKSSSFLFLPEDGLGKENQSYANTVKNKPSPEKKVDRIISPLKKSPLKQEFLEKNSSP 240
           VPGKSSSFLFLPEDGLGKENQSYANTVKNKPSPEKKVDRIISPLKKSPLKQEFLEKNSSP
Sbjct: 196 VPGKSSSFLFLPEDGLGKENQSYANTVKNKPSPEKKVDRIISPLKKSPLKQEFLEKNSSP 255

Query: 241 MKSQLEFRLERERAKDNSSNLSDDTEASSSPNKISEDIVKCLSSIFIRLSSSKDKATDSS 300
           MKSQLEFRLERERAKDNSSNLSDDTEASSSPNKISEDIVKCLSSIFIRLSSSKDKATDSS
Sbjct: 256 MKSQLEFRLERERAKDNSSNLSDDTEASSSPNKISEDIVKCLSSIFIRLSSSKDKATDSS 315

Query: 301 DTSSGPAELQDPYDACSDFKPRNIGPYRHLCAIEASSVDLDRSTNAVFLIHRLKNLFRRL 360
           DTSSGPAELQDPYDACSDFKPRNIGPYRHLCAIEASSVDLDRSTNAVFLIHRLKNLFRRL
Sbjct: 316 DTSSGPAELQDPYDACSDFKPRNIGPYRHLCAIEASSVDLDRSTNAVFLIHRLKNLFRRL 375

Query: 361 ASVNLAGLNHQEKLAFWINTYNSCMMNAFLEQGIPETHERVVTLMQKATIIVGGHLLNAI 420
           ASVNLAGLNHQEKLAFWINTYNSCMMNAFLEQGIPETHERVVTLMQKATIIVGGHLLNAI
Sbjct: 376 ASVNLAGLNHQEKLAFWINTYNSCMMNAFLEQGIPETHERVVTLMQKATIIVGGHLLNAI 435

Query: 421 TIEHFILRLPYHLKFTCPKAVKNDEMRARSVFGLEYSEPLITFALCCGSWSSPAVRVYSG 480
           TIEHFILRLPYHLKFTCPKAVKNDEMRARSVFGLEYSEPLITFALCCGSWSSPAVRVYSG
Sbjct: 436 TIEHFILRLPYHLKFTCPKAVKNDEMRARSVFGLEYSEPLITFALCCGSWSSPAVRVYSG 495

Query: 481 CKVEEELEVAKREYLQAAVGISKTNNKLMIPKVLDWYLLDFAKDLESMLDWICLQLPNEL 540
           CKVEEELEVAKREYLQAAVGISKTNNKLMIPKVLDWYLLDFAKDLESMLDWICLQLPNEL
Sbjct: 496 CKVEEELEVAKREYLQAAVGISKTNNKLMIPKVLDWYLLDFAKDLESMLDWICLQLPNEL 555

Query: 541 RIEAVKCLERKGREPLSQLVQVMPYNFSFRMLLHR 576
           RIEAVKCLERKGREPLSQLVQVMPYNFSFRMLLHR
Sbjct: 556 RIEAVKCLERKGREPLSQLVQVMPYNFSFRMLLHR 590

BLAST of Cucsa.102320.4 vs. NCBI nr
Match: gi|778718353|ref|XP_011657855.1| (PREDICTED: uncharacterized protein LOC101217980 isoform X4 [Cucumis sativus])

HSP 1 Score: 1143.6 bits (2957), Expect = 0.0e+00
Identity = 575/575 (100.00%), Postives = 575/575 (100.00%), Query Frame = 1

Query: 1   MGNDKPGTIINRRKLNRERKIALLQDVDKLKKKLRHEENVHKALERAFTRPLGALPRLPP 60
           MGNDKPGTIINRRKLNRERKIALLQDVDKLKKKLRHEENVHKALERAFTRPLGALPRLPP
Sbjct: 15  MGNDKPGTIINRRKLNRERKIALLQDVDKLKKKLRHEENVHKALERAFTRPLGALPRLPP 74

Query: 61  YLPPYILELLAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYVSSSSRNSDISTDTMEPIST 120
           YLPPYILELLAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYVSSSSRNSDISTDTMEPIST
Sbjct: 75  YLPPYILELLAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYVSSSSRNSDISTDTMEPIST 134

Query: 121 RIAKHRRTKSYCQNEFNSANSIARLQPSLARCSSSRKLLSNDTFFDRNGNGSNRFANGKH 180
           RIAKHRRTKSYCQNEFNSANSIARLQPSLARCSSSRKLLSNDTFFDRNGNGSNRFANGKH
Sbjct: 135 RIAKHRRTKSYCQNEFNSANSIARLQPSLARCSSSRKLLSNDTFFDRNGNGSNRFANGKH 194

Query: 181 VPGKSSSFLFLPEDGLGKENQSYANTVKNKPSPEKKVDRIISPLKKSPLKQEFLEKNSSP 240
           VPGKSSSFLFLPEDGLGKENQSYANTVKNKPSPEKKVDRIISPLKKSPLKQEFLEKNSSP
Sbjct: 195 VPGKSSSFLFLPEDGLGKENQSYANTVKNKPSPEKKVDRIISPLKKSPLKQEFLEKNSSP 254

Query: 241 MKSQLEFRLERERAKDNSSNLSDDTEASSSPNKISEDIVKCLSSIFIRLSSSKDKATDSS 300
           MKSQLEFRLERERAKDNSSNLSDDTEASSSPNKISEDIVKCLSSIFIRLSSSKDKATDSS
Sbjct: 255 MKSQLEFRLERERAKDNSSNLSDDTEASSSPNKISEDIVKCLSSIFIRLSSSKDKATDSS 314

Query: 301 DTSSGPAELQDPYDACSDFKPRNIGPYRHLCAIEASSVDLDRSTNAVFLIHRLKNLFRRL 360
           DTSSGPAELQDPYDACSDFKPRNIGPYRHLCAIEASSVDLDRSTNAVFLIHRLKNLFRRL
Sbjct: 315 DTSSGPAELQDPYDACSDFKPRNIGPYRHLCAIEASSVDLDRSTNAVFLIHRLKNLFRRL 374

Query: 361 ASVNLAGLNHQEKLAFWINTYNSCMMNAFLEQGIPETHERVVTLMQKATIIVGGHLLNAI 420
           ASVNLAGLNHQEKLAFWINTYNSCMMNAFLEQGIPETHERVVTLMQKATIIVGGHLLNAI
Sbjct: 375 ASVNLAGLNHQEKLAFWINTYNSCMMNAFLEQGIPETHERVVTLMQKATIIVGGHLLNAI 434

Query: 421 TIEHFILRLPYHLKFTCPKAVKNDEMRARSVFGLEYSEPLITFALCCGSWSSPAVRVYSG 480
           TIEHFILRLPYHLKFTCPKAVKNDEMRARSVFGLEYSEPLITFALCCGSWSSPAVRVYSG
Sbjct: 435 TIEHFILRLPYHLKFTCPKAVKNDEMRARSVFGLEYSEPLITFALCCGSWSSPAVRVYSG 494

Query: 481 CKVEEELEVAKREYLQAAVGISKTNNKLMIPKVLDWYLLDFAKDLESMLDWICLQLPNEL 540
           CKVEEELEVAKREYLQAAVGISKTNNKLMIPKVLDWYLLDFAKDLESMLDWICLQLPNEL
Sbjct: 495 CKVEEELEVAKREYLQAAVGISKTNNKLMIPKVLDWYLLDFAKDLESMLDWICLQLPNEL 554

Query: 541 RIEAVKCLERKGREPLSQLVQVMPYNFSFRMLLHR 576
           RIEAVKCLERKGREPLSQLVQVMPYNFSFRMLLHR
Sbjct: 555 RIEAVKCLERKGREPLSQLVQVMPYNFSFRMLLHR 589

BLAST of Cucsa.102320.4 vs. NCBI nr
Match: gi|778718347|ref|XP_011657852.1| (PREDICTED: uncharacterized protein LOC101217980 isoform X2 [Cucumis sativus])

HSP 1 Score: 1143.6 bits (2957), Expect = 0.0e+00
Identity = 575/575 (100.00%), Postives = 575/575 (100.00%), Query Frame = 1

Query: 1   MGNDKPGTIINRRKLNRERKIALLQDVDKLKKKLRHEENVHKALERAFTRPLGALPRLPP 60
           MGNDKPGTIINRRKLNRERKIALLQDVDKLKKKLRHEENVHKALERAFTRPLGALPRLPP
Sbjct: 31  MGNDKPGTIINRRKLNRERKIALLQDVDKLKKKLRHEENVHKALERAFTRPLGALPRLPP 90

Query: 61  YLPPYILELLAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYVSSSSRNSDISTDTMEPIST 120
           YLPPYILELLAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYVSSSSRNSDISTDTMEPIST
Sbjct: 91  YLPPYILELLAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYVSSSSRNSDISTDTMEPIST 150

Query: 121 RIAKHRRTKSYCQNEFNSANSIARLQPSLARCSSSRKLLSNDTFFDRNGNGSNRFANGKH 180
           RIAKHRRTKSYCQNEFNSANSIARLQPSLARCSSSRKLLSNDTFFDRNGNGSNRFANGKH
Sbjct: 151 RIAKHRRTKSYCQNEFNSANSIARLQPSLARCSSSRKLLSNDTFFDRNGNGSNRFANGKH 210

Query: 181 VPGKSSSFLFLPEDGLGKENQSYANTVKNKPSPEKKVDRIISPLKKSPLKQEFLEKNSSP 240
           VPGKSSSFLFLPEDGLGKENQSYANTVKNKPSPEKKVDRIISPLKKSPLKQEFLEKNSSP
Sbjct: 211 VPGKSSSFLFLPEDGLGKENQSYANTVKNKPSPEKKVDRIISPLKKSPLKQEFLEKNSSP 270

Query: 241 MKSQLEFRLERERAKDNSSNLSDDTEASSSPNKISEDIVKCLSSIFIRLSSSKDKATDSS 300
           MKSQLEFRLERERAKDNSSNLSDDTEASSSPNKISEDIVKCLSSIFIRLSSSKDKATDSS
Sbjct: 271 MKSQLEFRLERERAKDNSSNLSDDTEASSSPNKISEDIVKCLSSIFIRLSSSKDKATDSS 330

Query: 301 DTSSGPAELQDPYDACSDFKPRNIGPYRHLCAIEASSVDLDRSTNAVFLIHRLKNLFRRL 360
           DTSSGPAELQDPYDACSDFKPRNIGPYRHLCAIEASSVDLDRSTNAVFLIHRLKNLFRRL
Sbjct: 331 DTSSGPAELQDPYDACSDFKPRNIGPYRHLCAIEASSVDLDRSTNAVFLIHRLKNLFRRL 390

Query: 361 ASVNLAGLNHQEKLAFWINTYNSCMMNAFLEQGIPETHERVVTLMQKATIIVGGHLLNAI 420
           ASVNLAGLNHQEKLAFWINTYNSCMMNAFLEQGIPETHERVVTLMQKATIIVGGHLLNAI
Sbjct: 391 ASVNLAGLNHQEKLAFWINTYNSCMMNAFLEQGIPETHERVVTLMQKATIIVGGHLLNAI 450

Query: 421 TIEHFILRLPYHLKFTCPKAVKNDEMRARSVFGLEYSEPLITFALCCGSWSSPAVRVYSG 480
           TIEHFILRLPYHLKFTCPKAVKNDEMRARSVFGLEYSEPLITFALCCGSWSSPAVRVYSG
Sbjct: 451 TIEHFILRLPYHLKFTCPKAVKNDEMRARSVFGLEYSEPLITFALCCGSWSSPAVRVYSG 510

Query: 481 CKVEEELEVAKREYLQAAVGISKTNNKLMIPKVLDWYLLDFAKDLESMLDWICLQLPNEL 540
           CKVEEELEVAKREYLQAAVGISKTNNKLMIPKVLDWYLLDFAKDLESMLDWICLQLPNEL
Sbjct: 511 CKVEEELEVAKREYLQAAVGISKTNNKLMIPKVLDWYLLDFAKDLESMLDWICLQLPNEL 570

Query: 541 RIEAVKCLERKGREPLSQLVQVMPYNFSFRMLLHR 576
           RIEAVKCLERKGREPLSQLVQVMPYNFSFRMLLHR
Sbjct: 571 RIEAVKCLERKGREPLSQLVQVMPYNFSFRMLLHR 605

BLAST of Cucsa.102320.4 vs. NCBI nr
Match: gi|659079441|ref|XP_008440259.1| (PREDICTED: uncharacterized protein LOC103484766 isoform X2 [Cucumis melo])

HSP 1 Score: 1115.9 bits (2885), Expect = 0.0e+00
Identity = 559/575 (97.22%), Postives = 569/575 (98.96%), Query Frame = 1

Query: 1   MGNDKPGTIINRRKLNRERKIALLQDVDKLKKKLRHEENVHKALERAFTRPLGALPRLPP 60
           MGNDKPGTI+NRRKLNRERKIALLQDVDKLKKKLRHEENVHKALERAFTRPLGALPRLPP
Sbjct: 31  MGNDKPGTIVNRRKLNRERKIALLQDVDKLKKKLRHEENVHKALERAFTRPLGALPRLPP 90

Query: 61  YLPPYILELLAEVAVLEEEVVRLEEQVVNFRQGLYQEAVYVSSSSRNSDISTDTMEPIST 120
           YLPPYILELLAEVAVLEEEVVRLEEQVVNFRQGLYQEAV+VSSSSRNSDISTDTMEPIST
Sbjct: 91  YLPPYILELLAEVAVLEEEVVRLEEQVVNFRQGLYQEAVFVSSSSRNSDISTDTMEPIST 150

Query: 121 RIAKHRRTKSYCQNEFNSANSIARLQPSLARCSSSRKLLSNDTFFDRNGNGSNRFANGKH 180
           RIAKHRRTKSYCQNEFNSANSIARLQPSLARCSSSRKLLSNDTFFDRNGN SNRF++GKH
Sbjct: 151 RIAKHRRTKSYCQNEFNSANSIARLQPSLARCSSSRKLLSNDTFFDRNGNCSNRFSSGKH 210

Query: 181 VPGKSSSFLFLPEDGLGKENQSYANTVKNKPSPEKKVDRIISPLKKSPLKQEFLEKNSSP 240
           VPGK SSFLFLPEDGLGKENQSYANTVKNKPSPEKKVDRII+PLKKSPLKQEFLEKNSSP
Sbjct: 211 VPGKLSSFLFLPEDGLGKENQSYANTVKNKPSPEKKVDRIITPLKKSPLKQEFLEKNSSP 270

Query: 241 MKSQLEFRLERERAKDNSSNLSDDTEASSSPNKISEDIVKCLSSIFIRLSSSKDKATDSS 300
           MKSQLEFRLERERAKD+S +LSDDTEASSSPNKISEDIVKCLSSIFIRLSSSKDKATDSS
Sbjct: 271 MKSQLEFRLERERAKDSSCSLSDDTEASSSPNKISEDIVKCLSSIFIRLSSSKDKATDSS 330

Query: 301 DTSSGPAELQDPYDACSDFKPRNIGPYRHLCAIEASSVDLDRSTNAVFLIHRLKNLFRRL 360
           DTS+G AELQDPYDACSDFKPRNIG YRHLCAIEASSVDLDRSTNAVFLIHRLKNLFRRL
Sbjct: 331 DTSNGSAELQDPYDACSDFKPRNIGAYRHLCAIEASSVDLDRSTNAVFLIHRLKNLFRRL 390

Query: 361 ASVNLAGLNHQEKLAFWINTYNSCMMNAFLEQGIPETHERVVTLMQKATIIVGGHLLNAI 420
           ASVNLAGLNHQEKLAFWINTYNSCMMNAFLEQGIPETHERVVTLMQKATI+VGGHLLNAI
Sbjct: 391 ASVNLAGLNHQEKLAFWINTYNSCMMNAFLEQGIPETHERVVTLMQKATIVVGGHLLNAI 450

Query: 421 TIEHFILRLPYHLKFTCPKAVKNDEMRARSVFGLEYSEPLITFALCCGSWSSPAVRVYSG 480
           TIEHFILRLPYHLKFTCPKAVKNDEMRARSVFGLEYSEPLITFALCCGSWSSPAVRVYSG
Sbjct: 451 TIEHFILRLPYHLKFTCPKAVKNDEMRARSVFGLEYSEPLITFALCCGSWSSPAVRVYSG 510

Query: 481 CKVEEELEVAKREYLQAAVGISKTNNKLMIPKVLDWYLLDFAKDLESMLDWICLQLPNEL 540
           C VEEELEVAKREYLQAAVGISKTNNKLMIPKVLDWYLLDFAKDLESMLDWICLQLPNEL
Sbjct: 511 CNVEEELEVAKREYLQAAVGISKTNNKLMIPKVLDWYLLDFAKDLESMLDWICLQLPNEL 570

Query: 541 RIEAVKCLERKGREPLSQLVQVMPYNFSFRMLLHR 576
           RIEA+KCLERKGREPLSQLVQVMPYNFSFRMLLHR
Sbjct: 571 RIEAIKCLERKGREPLSQLVQVMPYNFSFRMLLHR 605

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A0A0KLB6_CUCSA0.0e+00100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_6G490260 PE=4 SV=1[more]
B9HE62_POPTR1.2e-21468.38Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0007s11280g PE=4 SV=2[more]
U5G8B7_POPTR1.2e-21468.38Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0007s11280g PE=4 SV=1[more]
A0A0D2TZ30_GOSRA2.4e-21267.37Uncharacterized protein OS=Gossypium raimondii GN=B456_008G074600 PE=4 SV=1[more]
A0A0D2RBE6_GOSRA2.4e-21267.37Uncharacterized protein OS=Gossypium raimondii GN=B456_008G074600 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G37080.28.5e-18560.10 Protein of unknown function, DUF547[more]
AT5G42690.24.3e-13650.26 Protein of unknown function, DUF547[more]
AT2G39690.12.0e-6439.58 Protein of unknown function, DUF547[more]
AT3G12540.18.3e-5535.57 Protein of unknown function, DUF547[more]
AT5G60720.11.7e-4437.38 Protein of unknown function, DUF547[more]
Match NameE-valueIdentityDescription
gi|778718344|ref|XP_011657851.1|0.0e+00100.00PREDICTED: uncharacterized protein LOC101217980 isoform X1 [Cucumis sativus][more]
gi|778718350|ref|XP_011657854.1|0.0e+00100.00PREDICTED: uncharacterized protein LOC101217980 isoform X3 [Cucumis sativus][more]
gi|778718353|ref|XP_011657855.1|0.0e+00100.00PREDICTED: uncharacterized protein LOC101217980 isoform X4 [Cucumis sativus][more]
gi|778718347|ref|XP_011657852.1|0.0e+00100.00PREDICTED: uncharacterized protein LOC101217980 isoform X2 [Cucumis sativus][more]
gi|659079441|ref|XP_008440259.1|0.0e+0097.22PREDICTED: uncharacterized protein LOC103484766 isoform X2 [Cucumis melo][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR006869DUF547
IPR025757MIP1_Leuzipper
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsa.102320Cucsa.102320gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsa.102320.4Cucsa.102320.4-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.102320.4.CDS.9Cucsa.102320.4.CDS.9CDS
Cucsa.102320.4.CDS.8Cucsa.102320.4.CDS.8CDS
Cucsa.102320.4.CDS.7Cucsa.102320.4.CDS.7CDS
Cucsa.102320.4.CDS.6Cucsa.102320.4.CDS.6CDS
Cucsa.102320.4.CDS.5Cucsa.102320.4.CDS.5CDS
Cucsa.102320.4.CDS.4Cucsa.102320.4.CDS.4CDS
Cucsa.102320.4.CDS.3Cucsa.102320.4.CDS.3CDS
Cucsa.102320.4.CDS.2Cucsa.102320.4.CDS.2CDS
Cucsa.102320.4.CDS.1Cucsa.102320.4.CDS.1CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.102320.4.three_prime_UTR.1Cucsa.102320.4.three_prime_UTR.1three_prime_UTR


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.102320.4.five_prime_UTR.1Cucsa.102320.4.five_prime_UTR.1five_prime_UTR


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006869Domain of unknown function DUF547PFAMPF04784DUF547coord: 367..495
score: 5.3
IPR025757Ternary complex factor MIP1, leucine-zipperPFAMPF14389Lzipper-MIP1coord: 17..97
score: 7.9
NoneNo IPR availableunknownCoilCoilcoord: 66..93
score: -coord: 20..47
scor
NoneNo IPR availablePANTHERPTHR23054UNCHARACTERIZEDcoord: 1..105
score: 1.7E-267coord: 156..575
score: 1.7E
NoneNo IPR availablePANTHERPTHR23054:SF29EXPRESSED PROTEINcoord: 156..575
score: 1.7E-267coord: 1..105
score: 1.7E