Cucsa.085230.1 (mRNA) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.085230.1
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Alpha/beta-Hydrolases superfamily protein, putative
Location	scaffold00867 : 92691 .. 98722 (-)
Sequence length	1199

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGTTGCCTTTCTTCTTCTTCTTCTTCTTCTTTACCTCACTAACAATGGCTTCTTCAACAAACAAAAACGATGATGTAGCCTTCGATTTCTTTCCCTTTTTAAGAGTCTACACTGACGGTCGCGTCCAACGACTCATGACCACCTCCGATATTGTTCCCGCCGACGCCGACGACCCTAAATCCCCTTTCCGATCCAAAGACGTCACCATCTCGACAGATCCTGCTGTGTCGGCTCGTGTTTTCATCCCCTCCTCCGCTGATCCAAATCAAAAGCTGCCCCTCCTCTTGTACGTCCACGGCGGAGCCTTCTGCATCGAATCGGCTTTCAGTTTGCAGTACCACCAGCACGTGGGCTCTCTTGCTGCCAAGGCCAACGCCGTTGCGGTCTCCGTTGAATACCGCCTCGCGCCGGAGCATCCTATCCCCGCCTGCTACGAGGACTGTTGGGATGCTCTACGGTGGGTTGCGGCACATGTGAACAGGTAAAGTAAAGTATATCTCAATTTATTATTATTATTATTATTATTATTTTAATTTTACTTTATTGTTTTGCAAATATGAATTTATATTTTTCAAAAACAAAACTAAAATTTAGATAAGAGAAGACAAATGCTTAGAGATTTAGGTAGGCAAATTGCAAGATTGACAAACTTTCATAATAAAACATACATGATCCTTCTACTTATACCCAAAATTAAAATTGAATTTAGGACACGTTTAGTAACGTTCTTGTTTTCTGTTTCTTATTTCTTGTTTCATGTTCCTAGGTTTCCCTTACTTCTTCTATTAGATCAAAAGATAAAAACGGGTTTGATAATTGTTTCTTGTTTCTTGATTTTTTTAGATTTATTTTTTATTTTATTCAAATTTAATTCAATTAAACATCAAACAAAAATATATGTCCTCTATTAAACCTAAAAAATAAAATAAAATTATCAAACATAGATTAGAAAGTCTTCTTGTAATCTTATGGAGAACGAGATGAAAGATTGAGATATGAAGGATAGAGCTTACGAGAGGAAGACAAAAACCCACAATGAAGAGGAGGACTGAAACCCATGGTAAAGAGAAAGACAAAAATCGACAGCGAAAACACAATTCACGCAAAGAGGAAGACAATGAGAGATGAAGAGGAAGACTGTGAAGACAATGAGAGGAAGAGAAAGATGATGGTTATTTGGAAAAGAGATGGAAATGAGAGGAAGAAGAGAAATATATGTGTTCTTTGTATTGAAAAAAGAGGAAGAAAATGAAGAAAAAAACCGACGTGGAGAGTGGTTATTTTGGGAAAGAAGAAGAAAGAAAAGAAAATTACTAGAGGAAAATGAAATAGAAAAATAATTGAAAAAAAGAAAAGAAAACCAATAAAAAGAATTGAGAAAAAAGAAAATGAAATTAATAAAAAGAATTTGAGAAAAATGAAAAGAAAACCAATAAAAATAATTGAGAAAAAAAAACTAATAGAAGTAATTGACAGTCACGGAAGAAACTATTTTTTTCAAACAATTTTTTATAAAATGATAAACGTTTCTATTTTTTTTAGAACAAGAAACATGAAACGTTACCAAACACTTCTATTTTTTTAAAAATGAAAACAGAAACAGGAAACAAGAAACGAAAACATTACCAAGCACACCTTTAATTTTTCAAGTTTCAATTTCAAAGCTTGTATAAGTGTTTGAGAGTTCAATTTTTTTAATTGAAATTTTTGAATGTAACTACAAATTTTCCAAATCACATAAACATTGAAAAGCAACCTTAAAAACTATCATACTTCACAACTAGCTGGGATTCTTTGGAAGAAATAGTCAAAATCAATTTTTTCAATAGTTATGGATTTAGTGGAAGATTGGAATAAAAATTACTAACATCTAAATTAAATATCAATTTTCTTTTAACAAAACTTAATCAATTTTTAACATTTGAGAAATATTTAATTTTGGTTTCAGTGTTAATGGAATAACTTAAGTATTTGATGAAAGCTGATGTCCAATTTGAAATATTAATAATAGTAACGATGTTTGAGAGGAGATACTCAAAATTAACAAAATAGAATATTTGACAAAATATTTATTAATTTTTTTGTTTGGGTCGGATTGGTTTTATGTTTTATGGAATGTATTAAAATTTTCACATTTTTGCAAAAAAACTCTATTATAATAATTACTTTAAAAAAAAACATGATTTCTACTCTTTTGGGTTTTATTTGATTAAATTTAAACTTTATTTTTCTAAGTATATTAATTTATACTATCTTTCAAATTTGATTTGATCAATAATGTGCATGAAATGTGTGATTTAAATCCACTAAATTTTTAAATTAATATTGTACCGATCAAACTTTTCAAATATAAAATGAGGTTATTACCTAAAAGAAATAACATTTTCGAACTTTTAAAATAACGCAAGGTCTACCCATTTTATTAAATAAAAGATAAAACAAACCATCTGAAAATAAGCGTTTGACAAACCATTAAAAATTACTAAGAAAATAAACCAATTATTCGGGCCCTATCTAGGTTTGAAGAAAATATAACGTAAATGTTAACCAAACTCTTTAACAAACGGGAGAAAAAAATATCGCAATGAAATCATGAAAATCATGGAAATAACGTAAGAAAAACATAAGCAAGAACGTTAAGTTCACATATGACAAGTCACAGGTCCTTGTCGCTCGATAACTTGTACGTTTCTTTACCTTTGTCTAGAAAGTTAAACATAGAGAAAGAGTGAGTATAAAAATACTACATAATGGATCCACTACTAGTCCTACTGGCTTAGCCGTTAACTTTCTATTAAGATGTATCATGGGTCATATAGTCTGGTAATCCCGTAAGAACACACATCAATCATAAAGTGAACTGAAAGGCACAATTAGTAGTGACCAATTAGTCATCAATATGAGTTTAAAAGTGGTGATCTCAAGAGACACAGTACATGTAGATTATAGCCTATTAACCAAAACTAACAGTTATTAACAACTACCAACAGTTTAAATGAGCCTACTATCGTCAAACATATAGTCTAATAGTAGGTATAAACATAACATGCAAACATCATCCAACAAACTCCTCAATTACATAATCAGTTACATAAATAAATCAACACATTCTCAGTCAAACCCCCACTTAAGCCTGTCAATGCAATTTAATTTTCGTCAATGAACATTTTAACTAGTCAATCCATACAATATGATTCCATCTTGATTCGAGGGTCCGAGGATAGAAATCCCATAGCTTAAAAAGAATTAAATTCGTGTGCACAACTCTTGATTCAACTTAAACCACAATTTCATCCTATCATGGTCAAAGTTAAATCAATAATTTTGCCCACGAAAATTGTCAATTTAATTAACCTTGGCTTTCCCCACTTATCCAAATTGAAGTTGAACCAAATTTCAAACTTAGCTTGATGGGAATTAAGAATCAAATTCAAATGGCTCCAATTCCATCTTCAGAGATCGAGAAGCTCAAAAATTAAATTCAATGAATTAGATCTTAAAATGTTTACTAAATGGTAATCAAAACCCCATAAAACCACATCTTACTCACCCTTGGACCAAAATACAAGATCGACAAGCGATGGCATGACCGGAGGAGCGACAAACAGAGTTAAATCGAATGAAGGACCCACAACGGTGGGCAACACGAAGTTGACACAGACAACTTGTACTAGAAGGGTGCTCGACTAGGCTCCTTGTTCATGACATCAAATGAACGAAGGAGAAGTCAATGCAGACAGTTAACCAATTATAAATAAATAAATATATATATATATATATACACAAACTAAAGGGTAATTGTGATGATGGTCATTTTAGGAATAATAGTTAAGAATATAGCAATATTTAATTTTTTTTGTAAATATAACAAAATTATCGCGAATGGACTCGTATGGTCTATCAGTAATAAACCAATATTTACACTATAGTCGATCGGTGATAGATTTATATATCATTGATAGAATTTGAAAATTTTTACTATATTTACAAATTATAAATTTTTAAAATATTATTATATACTTAATTATTTCGAATATAATTACTAATTTGTACTTATTGATTGGTTAATACCAATTCTCTTTCTTCATTTAATTCCTTATCTCATCACCAACCATCACACCAACCCATCCCTCGTTAAATATTTTCCAACGAGTATCTTTCTTTTCACCATCCAATATATATATATATACACAATCAATGATAGTTCCACCAAAATCCACATTCTTAAATCTTAATTAAATATACATAAAACACGTGTGCTTAAATAATTTCAGATAAAATAACACCCAATACCAACTCAAACGTAAAATAAAATTAAACCCAATACCAACACAAACATAAAATATAATTAAAAATTACTTTAATTTTAAAGTGTTACAAGTATGGCAGGTACACTTCTTCCAATATTGATTTTACAAAATGTAATATCGAAGACGATATGTTTGAACGGTTTCATAGAAAATTTAGTCTAAGAGTCTATATTAATTAATTAATTTATATAAATTTAAGGGTTTAAGTGATATAATCATAAGTTTCATAAAGAGTTTTTTTCGAATGAAAAGGGTACAAATCAATACACTTAACAAAAATTCATGGTCTAAATTGTTACAATAATAAATTAAGATTTTTATAGTCTTGTGAGAGGTCACGTCACAAAAATCTCTTAAGTATTTAAGATCAAGAATATGAAGTATTTATAAAATGCCAGGAAATCAATTGTTTATAGATCATTTTTTAAAATGGGCTTCAATGATAATCAAAGCTAAACTTAACTGCCATGTAAATTATTTTCCTAAAAAAATTGAATTAAACTATCAAAATTATACTAAATATTAGAGAGACATTAAAAAAAACCATAAAAGTTAGCTTCTAAAGTTATAGATATCAAATTAAACTAAATTTAGAGTATCAAACACTATATAGTTAATTTGAATCAAAGTTGCAGTATCTTGATATTGAGTGTATTTGGTTTAAGTTTTTTAGTGTGTAATTTTAAAATTAGTCCGTTAGAAAAAGTTAAAATATTTTTGACAACTATTTAAAATAACTTTAACCTAAGTTTTAAAATAGTATTTTAAATGGTTAATCAAAAGAATTTTTTTGACATTTTTTTTATATAATCATTCAAACAAATAACCCTTAAACATCTAGTATTAATTTAGACCTAAATTAGTTTTAATTAACTAACTTCTGAAATATATTGAAATAATTATATTTTTTGGACTTCATCAAGTGAGCTATATACATCTATGTATGCTTTCAAGTATAATGTGATAAGAGATTAAGTTGAGTTAAATCTTTCCATAAACAACAGAGATGGTTCAGAGCCATGGCTAAACACATATGTGGATTTCAACCGTATTTGTTTAGCTGGGGATAGTGCCGGTGCAAACATCTGCCATTATTTGGCTGCTCGAGCAAGTTCGTCGGCAGAGGAACTGGGTGGAGCGAAGGTAGTGGCAATGGCTTTGATTCACCCGTTTTTTGGTGATGGGGGAGAGAATAGGCTGTGGAAGTATCTGTGTTCGGAGACAAAGTTACTGAGACCAACCATAGAGGATCTGGCCAAGTTGGGTTGTAAGAGAGTGAAGATTTTTTTAGCGGAAAATGATTTTCTGAAGTCTGGAGGGAAGAATTACGAGGAAGATTTGAAGAGCAGCGGGTGGAATGGGACGGTGGAGACGGTGGAGCACGGCGAAGAAAATCATGTGTTTCATTTGAAGAAACCTGAGTGTGAAAAGGCTGTTGATTTGTTGGAAAAATTAGCCTCTTTCATTAACTTAGATTAGAAACATTGTTTATGTTTATTCCAAATATTTGAAAGACATTTGAATAATTATCCTTGTCAAGGTAGGTGGGTATGGAGGAGGAGGGAAAGAGTTACGGAGGAAAAGTATTATGATAATTCTAATATTCTTAGTTTTATAATAATATGTGTTTGGGATTAGGGTTATTATTGGTAGTGTTATGATAATATATGTTTGGGTGAAGAAATATGAATGGTAGTGTTATGATAATATGTGTTTTGGGGA

mRNA sequence

GGTTGCCTttcttcttcttcttcttcttcttTACCTCACTAACAATGGCTTCTTCAACAAACAAAAACGATGATGTAGCCTTCGATTTCTTTCCCTTTTTAAGAGTCTACACTGACGGTCGCGTCCAACGACTCATGACCACCTCCGATATTGTTCCCGCCGACGCCGACGACCCTAAATCCCCTTTCCGATCCAAAGACGTCACCATCTCGACAGATCCTGCTGTGTCGGCTCGTGTTTTCATCCCCTCCTCCGCTGATCCAAATCAAAAGCTGCCCCTCCTCTTGTACGTCCACGGCGGAGCCTTCTGCATCGAATCGGCTTTCAGTTTGCAGTACCACCAGCACGTGGGCTCTCTTGCTGCCAAGGCCAACGCCGTTGCGGTCTCCGTTGAATACCGCCTCGCGCCGGAGCATCCTATCCCCGCCTGCTACGAGGACTGTTGGGATGCTCTACGGTGGGTTGCGGCACATGTGAACAGAGATGGTTCAGAGCCATGGCTAAACACATATGTGGATTTCAACCGTATTTGTTTAGCTGGGGATAGTGCCGGTGCAAACATCTGCCATTATTTGGCTGCTCGAGCAAGTTCGTCGGCAGAGGAACTGGGTGGAGCGAAGGTAGTGGCAATGGCTTTGATTCACCCGTTTTTTGGTGATGGGGGAGAGAATAGGCTGTGGAAGTATCTGTGTTCGGAGACAAAGTTACTGAGACCAACCATAGAGGATCTGGCCAAGTTGGGTTGTAAGAGAGTGAAGATTTTTTTAGCGGAAAATGATTTTCTGAAGTCTGGAGGGAAGAATTACGAGGAAGATTTGAAGAGCAGCGGGTGGAATGGGACGGTGGAGACGGTGGAGCACGGCGAAGAAAATCATGTGTTTCATTTGAAGAAACCTGAGTGTGAAAAGGCTGTTGATTTGTTGGAAAAATTAGCCTCTTTCATTAACTTAGATTAGAAACATTGTTTATGTTTATTCCAAATATTTGAAAGACATTTGAATAATTATCCTTGTCAAGGTAGGTGGGTATGGAggaggagggaaagagttacggaggaaaagtattatgataattctaatattcttagttttataataatatgtgtttgggattagggttattattggtagtgttatgataatatatgtttgggtgaagaaatatgaatggtagtgttatgataatatgtgttttgggga

Coding sequence (CDS)

ATGGCTTCTTCAACAAACAAAAACGATGATGTAGCCTTCGATTTCTTTCCCTTTTTAAGAGTCTACACTGACGGTCGCGTCCAACGACTCATGACCACCTCCGATATTGTTCCCGCCGACGCCGACGACCCTAAATCCCCTTTCCGATCCAAAGACGTCACCATCTCGACAGATCCTGCTGTGTCGGCTCGTGTTTTCATCCCCTCCTCCGCTGATCCAAATCAAAAGCTGCCCCTCCTCTTGTACGTCCACGGCGGAGCCTTCTGCATCGAATCGGCTTTCAGTTTGCAGTACCACCAGCACGTGGGCTCTCTTGCTGCCAAGGCCAACGCCGTTGCGGTCTCCGTTGAATACCGCCTCGCGCCGGAGCATCCTATCCCCGCCTGCTACGAGGACTGTTGGGATGCTCTACGGTGGGTTGCGGCACATGTGAACAGAGATGGTTCAGAGCCATGGCTAAACACATATGTGGATTTCAACCGTATTTGTTTAGCTGGGGATAGTGCCGGTGCAAACATCTGCCATTATTTGGCTGCTCGAGCAAGTTCGTCGGCAGAGGAACTGGGTGGAGCGAAGGTAGTGGCAATGGCTTTGATTCACCCGTTTTTTGGTGATGGGGGAGAGAATAGGCTGTGGAAGTATCTGTGTTCGGAGACAAAGTTACTGAGACCAACCATAGAGGATCTGGCCAAGTTGGGTTGTAAGAGAGTGAAGATTTTTTTAGCGGAAAATGATTTTCTGAAGTCTGGAGGGAAGAATTACGAGGAAGATTTGAAGAGCAGCGGGTGGAATGGGACGGTGGAGACGGTGGAGCACGGCGAAGAAAATCATGTGTTTCATTTGAAGAAACCTGAGTGTGAAAAGGCTGTTGATTTGTTGGAAAAATTAGCCTCTTTCATTAACTTAGATTAG

Protein sequence

MASSTNKNDDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPAVSARVFIPSSADPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRLAPEHPIPACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAARASSSAEELGGAKVVAMALIHPFFGDGGENRLWKYLCSETKLLRPTIEDLAKLGCKRVKIFLAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVFHLKKPECEKAVDLLEKLASFINLD*

Homology

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BLAST of Cucsa.085230.1 vs. Swiss-Prot
Match: HIDM_GLYEC (2-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata GN=HIDM PE=1 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 1.9e-60
Identity = 124/326 (38.04%), Postives = 190/326 (58.28%), Query Frame = 1


Query: 2   ASSTNKNDDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPAV 61
           ++ST  + ++  +  P LRVY DG V+R + +S  VP   +DP++   +KD+ IS +P +
Sbjct: 4   STSTTTSKEIDRELPPLLRVYKDGTVERFLGSS-FVPPSPEDPETGVSTKDIVISENPTI 63

Query: 62  SARVFIPSSADPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRLA 121
           SARV++P   +  +KLP+L+Y HGGAFC+ESAFS  + +++  +A+KAN + VS+EYRLA
Sbjct: 64  SARVYLPKLNNTTEKLPILVYYHGGAFCLESAFSFLHQRYLNIVASKANVLVVSIEYRLA 123

Query: 122 PEHPIPACYEDCWDALRWVAAHVNRD----GSEPWLNTYVDFNRICLAGDSAGANICHYL 181
           PEHP+PA YED W AL+WV +H   +     ++PWL  + DFNR  + GD++GANI H  
Sbjct: 124 PEHPLPAAYEDGWYALKWVTSHSTNNNKPTNADPWLIKHGDFNRFYIGGDTSGANIAHNA 183

Query: 182 AARASSSAEELGGAKVVAMALIHPFFG----------DGGEN----RLWKYLCSET---- 241
           A R  + A   GG ++  +    P F           +G E     ++W ++  +     
Sbjct: 184 ALRVGAEALP-GGLRIAGVLSAFPLFWGSKPVLSEPVEGHEKSSPMQVWNFVYPDAPGGI 243

Query: 242 -----KLLRPTIEDLAKLGCKRVKIFLAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGE 301
                  L P   +LA LGC ++ +F+A  D L+  G  Y E +K SGW G VE  ++  
Sbjct: 244 DNPLINPLAPGAPNLATLGCPKMLVFVAGKDDLRDRGIWYYEAVKESGWKGDVELAQYEG 303

[Close]

BLAST of Cucsa.085230.1 vs. Swiss-Prot
Match: CXE12_ARATH (Probable carboxylesterase 12 OS=Arabidopsis thaliana GN=CXE12 PE=1 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 2.5e-60
Identity = 125/321 (38.94%), Postives = 186/321 (57.94%), Query Frame = 1


Query: 8   NDDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPAVSARVFI 67
           + ++A D  P L++Y  GR++RLM  + + P+   +P++   SKDV  S D  +S R+++
Sbjct: 2   DSEIAVDCSPLLKIYKSGRIERLMGEATVPPSS--EPQNGVVSKDVVYSADNNLSVRIYL 61

Query: 68  P--SSADPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRLAPEHP 127
           P  ++A+ + KLPLL+Y HGG F IE+AFS  YH  + +  + +N VAVSV+YR APEHP
Sbjct: 62  PEKAAAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHP 121

Query: 128 IPACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAARASSS- 187
           I   ++D W AL+WV  H+   G E WLN + DF+R+ L+GDSAGANI H++A RA+   
Sbjct: 122 ISVPFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEK 181

Query: 188 -AEELGGAKVVAMALIHPFFG--------DGGENRL-------WKYLCSETK-------- 247
            +  L    +  + L+HP+F         D  +  L       W      +K        
Sbjct: 182 LSPGLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLL 241

Query: 248 -LLRPTIEDLAKLGCKRVKIFLAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVF 301
            +++    DL+ LGC +V + +AE D L   G  Y   L+ SGW G VE VE   E+HVF
Sbjct: 242 NVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVF 301

[Close]

BLAST of Cucsa.085230.1 vs. Swiss-Prot
Match: CXE1_ARATH (Probable carboxylesterase 1 OS=Arabidopsis thaliana GN=CXE1 PE=2 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 1.6e-59
Identity = 120/314 (38.22%), Postives = 178/314 (56.69%), Query Frame = 1


Query: 8   NDDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPAVSARVFI 67
           + ++AFD+ P  R++ +G ++RL+  + + P+   +P++   SKD   S +  +S R+++
Sbjct: 2   DSEIAFDYSPRFRIFKNGGIERLVPETFVPPSL--NPENGVVSKDAVYSPEKNLSLRIYL 61

Query: 68  PSSA---DPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRLAPEH 127
           P ++      +K+PLL+Y HGG F +E+AFS  YH  + S  +  + +AVSVEYR APEH
Sbjct: 62  PQNSVYETGEKKIPLLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAPEH 121

Query: 128 PIPACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAARASSS 187
           PIP  YED WDA++W+  H+ R G E WLN + DF+++ LAGDSAGANI H++A R    
Sbjct: 122 PIPTLYEDSWDAIQWIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVDKE 181

Query: 188 AEELGGAKVVAMALIHPFFGDGG------------ENRLWKYLCS------ETKLLRPTI 247
                  K+  M L HP+F                  RLW+          E   +    
Sbjct: 182 KLPPENFKISGMILFHPYFLSKALIEEMEVEAMRYYERLWRIASPDSGNGVEDPWINVVG 241

Query: 248 EDLAKLGCKRVKIFLAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVFHLKKPEC 301
            DL  LGC+RV + +A ND L  GG +Y  +L+ SGW G V+ +E  EE HVFHL+ P+ 
Sbjct: 242 SDLTGLGCRRVLVMVAGNDVLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLRDPDS 301

[Close]

BLAST of Cucsa.085230.1 vs. Swiss-Prot
Match: CXE2_ARATH (Probable carboxylesterase 2 OS=Arabidopsis thaliana GN=CXE2 PE=2 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 8.0e-59
Identity = 133/320 (41.56%), Postives = 183/320 (57.19%), Query Frame = 1


Query: 4   STNKNDDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPAVSA 63
           ST K   V+ +  P+L V+TDG V+RL  T    P    DP +   SKD+ I     +SA
Sbjct: 3   STKKQ--VSLELLPWLVVHTDGTVERLAGTEVCPPGL--DPITGVFSKDIIIEPKTGLSA 62

Query: 64  RVFIPSSADPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRLAPE 123
           R++ P S  P QK+PL+LY HGGAF I S     YH  +  +  +AN +AVSV YRLAPE
Sbjct: 63  RIYRPFSIQPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPE 122

Query: 124 HPIPACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAARASS 183
           HP+P  YED W AL+ + A      +EPW+N Y D + + L GDSAGANI H+LA RA  
Sbjct: 123 HPLPTAYEDSWTALKNIQAI-----NEPWINDYADLDSLFLVGDSAGANISHHLAFRAKQ 182

Query: 184 SAEELGGAKVVAMALIHPFFGD----GGE----------NRLWKYLC-----SETKLLRP 243
           S + L   K+  + +IHP+F      G E          +  W+++C     S+   + P
Sbjct: 183 SDQTL---KIKGIGMIHPYFWGTQPIGAEIKDEARKQMVDGWWEFVCPSEKGSDDPWINP 242

Query: 244 TIE---DLAKLGCKRVKIFLAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVFHL 302
             +   DL  LGC+RV I +AE D L   GK Y E L  S W G VE +E  E++HVFH+
Sbjct: 243 FADGSPDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHI 302

[Close]

BLAST of Cucsa.085230.1 vs. Swiss-Prot
Match: HIDH_SOYBN (2-hydroxyisoflavanone dehydratase OS=Glycine max GN=HIDH PE=1 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.2e-57
Identity = 117/317 (36.91%), Postives = 180/317 (56.78%), Query Frame = 1


Query: 10  DVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPAVSARVFIPS 69
           ++  +  P +RVY DG V+RL++ S+ V A  +DP++   SKD+ I+ +P VSAR+F+P 
Sbjct: 4   EIVKELLPLIRVYKDGSVERLLS-SENVAASPEDPQTGVSSKDIVIADNPYVSARIFLPK 63

Query: 70  SADPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRLAPEHPIPAC 129
           S   N KLP+ LY HGGAFC+ESAFS   H+++  LA++AN +A+SV++RL P HPIPA 
Sbjct: 64  SHHTNNKLPIFLYFHGGAFCVESAFSFFVHRYLNILASEANIIAISVDFRLLPHHPIPAA 123

Query: 130 YEDCWDALRWVAAHVNRDGS---EPWLNTYVDFNRICLAGDSAGANICHYLAARASSSAE 189
           YED W  L+W+A+H N   +   EPWL  + DF ++ + G+++GANI H L  RA + + 
Sbjct: 124 YEDGWTTLKWIASHANNTNTTNPEPWLLNHADFTKVYVGGETSGANIAHNLLLRAGNESL 183

Query: 190 ELGGAKVVAMALIHPFFG----------DGGEN----RLWKYLCSET---------KLLR 249
             G  K++   L  PFF           +G E     ++W + C +              
Sbjct: 184 P-GDLKILGGLLCCPFFWGSKPIGSEAVEGHEQSLAMKVWNFACPDAPGGIDNPWINPCV 243

Query: 250 PTIEDLAKLGCKRVKIFLAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVFHLKK 301
           P    LA L C ++ + +   D  +     Y   ++ SGW G ++  + G+E H F L K
Sbjct: 244 PGAPSLATLACSKLLVTITGKDEFRDRDILYHHTVEQSGWQGELQLFDAGDEEHAFQLFK 303

[Close]

BLAST of Cucsa.085230.1 vs. TrEMBL
Match: A0A0A0LNZ1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G234530 PE=4 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 1.6e-175
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 1


Query: 1   MASSTNKNDDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPA 60
           MASSTNKNDDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPA
Sbjct: 1   MASSTNKNDDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPA 60

Query: 61  VSARVFIPSSADPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRL 120
           VSARVFIPSSADPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRL
Sbjct: 61  VSARVFIPSSADPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRL 120

Query: 121 APEHPIPACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAAR 180
           APEHPIPACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAAR
Sbjct: 121 APEHPIPACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAAR 180

Query: 181 ASSSAEELGGAKVVAMALIHPFFGDGGENRLWKYLCSETKLLRPTIEDLAKLGCKRVKIF 240
           ASSSAEELGGAKVVAMALIHPFFGDGGENRLWKYLCSETKLLRPTIEDLAKLGCKRVKIF
Sbjct: 181 ASSSAEELGGAKVVAMALIHPFFGDGGENRLWKYLCSETKLLRPTIEDLAKLGCKRVKIF 240

Query: 241 LAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVFHLKKPECEKAVDLLEKLASFI 300
           LAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVFHLKKPECEKAVDLLEKLASFI
Sbjct: 241 LAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVFHLKKPECEKAVDLLEKLASFI 300

Query: 301 NLD 304
           NLD
Sbjct: 301 NLD 303

[Close]

BLAST of Cucsa.085230.1 vs. TrEMBL
Match: A8TU55_PAESU (CXE carboxylesterase OS=Paeonia suffruticosa PE=2 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 3.4e-88
Identity = 162/301 (53.82%), Postives = 209/301 (69.44%), Query Frame = 1


Query: 5   TNKNDDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPAVSAR 64
           T   DDVA +FFPF RVY DGRV+R M T  + P D  DP +  RSKDV IS  P V+ R
Sbjct: 29  TPPGDDVAHEFFPFFRVYKDGRVERFMPTEKVPPTD--DPNTGVRSKDVQIS--PEVAVR 88

Query: 65  VFIPSSADPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRLAPEH 124
           +F+P   DP QK+P+L Y HGG F I SAF+  YH +V SL A+AN +AVSV+YRLAPEH
Sbjct: 89  IFLPKIDDPTQKVPVLFYTHGGGFSIGSAFAQGYHNYVSSLVAEANVIAVSVDYRLAPEH 148

Query: 125 PIPACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAARASSS 184
           PIPACYED W+A +WVA+H N +G EPWLN + DF R+ + GDSAGANI H LAAR  S+
Sbjct: 149 PIPACYEDSWEAFKWVASHANGNGPEPWLNDHADFRRVFMTGDSAGANITHTLAARIGST 208

Query: 185 AEELGGAKVVAMALIHPFFGDGGENRLWKYLCS-----ETKLLRPTIEDLAKLGCKRVKI 244
             EL G KV+ +AL+HP+FG   ++++W +LC      E   L+P  EDLAKLGC+++ I
Sbjct: 209 --ELPGVKVIGIALVHPYFGGTDDDKMWLFLCPTNGGLEDPRLKPATEDLAKLGCEKMLI 268

Query: 245 FLAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVFHLKKPECEKAVDLLEKLASF 301
           F+A+ D LK  G +Y ++LK SGW GTVE  E+  ++HVFHL  P C+ A  + ++L SF
Sbjct: 269 FVADEDHLKERGISYYDELKKSGWKGTVEIEENKGQHHVFHLMNPTCDDAKAMKKRLVSF 323

[Close]

BLAST of Cucsa.085230.1 vs. TrEMBL
Match: F6HQD6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_03s0063g00810 PE=4 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 4.5e-85
Identity = 158/305 (51.80%), Postives = 208/305 (68.20%), Query Frame = 1


Query: 2   ASSTNKNDDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPAV 61
           A     N  ++  FFPF ++Y DGRV+R M T  + P+D  DP +  RSKDV IS +  V
Sbjct: 11  AKLEKSNGQLSHLFFPFFKIYQDGRVERFMHTDHVPPSD--DPLTGVRSKDVIISPETGV 70

Query: 62  SARVFIPSSADPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRLA 121
           SAR+FIP   +PN KLPLL+Y+HGG F I+SAFS  Y+ +V SL A+AN +A+SV+YRLA
Sbjct: 71  SARLFIPKLPNPNCKLPLLIYIHGGGFSIQSAFSTSYNHYVKSLVAEANVIALSVDYRLA 130

Query: 122 PEHPIPACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAARA 181
           PEHPIPACY+D W A++W A+H N DG + WLN + DF+R+  AGDSAG NI + LA R 
Sbjct: 131 PEHPIPACYDDSWAAVQWAASHANGDGPDTWLNNHADFSRVFFAGDSAGGNISNTLAFRV 190

Query: 182 SSSAEELGGAKVVAMALIHPFFGDGGENRLWKYLCS-----ETKLLRPTIEDLAKLGCKR 241
            SS   L G KVV + L+HP+FG  G++++W Y+C      E   L+P  EDLA+LGC+R
Sbjct: 191 GSSG--LPGVKVVGVVLVHPYFGGTGDDQMWLYMCPNHGGLEDPRLKPGAEDLARLGCER 250

Query: 242 VKIFLAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVFHLKKPECEKAVDLLEKL 301
           V +F+AE D L+    +Y E LK S W GTVE VE+  E HVFHL  P+CE A  L++K+
Sbjct: 251 VLMFVAEKDHLRPVAWDYYEKLKKSEWKGTVEIVENHGEEHVFHLMNPKCENAAVLMKKI 310

[Close]

BLAST of Cucsa.085230.1 vs. TrEMBL
Match: A0A059BCP2_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_G01495 PE=4 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 1.3e-84
Identity = 152/305 (49.84%), Postives = 208/305 (68.20%), Query Frame = 1


Query: 1   MASSTNKNDDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPA 60
           M  + + N DV  +FFPF +VY DGRV+R+M    +    ++D +   RSKDVTIST+PA
Sbjct: 1   MEPTKSDNGDVLHEFFPFFKVYKDGRVERMMPMEKLKVPPSEDAE--VRSKDVTISTEPA 60

Query: 61  VSARVFIPSSADPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRL 120
           V ARVF P    P ++LP+L+Y+HGG F I SAF   YH++V  L A+A  VAVSVEYRL
Sbjct: 61  VLARVFAPRGTGPAERLPVLIYIHGGGFSIGSAFHRVYHEYVSLLMARARVVAVSVEYRL 120

Query: 121 APEHPIPACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAAR 180
           APE+PIPACY+D W A RW   H + +G +PWLN + DF R+ + GDSAGANICH +A R
Sbjct: 121 APENPIPACYDDAWAATRWALGHADGNGPDPWLNEHGDFGRVYITGDSAGANICHNVAVR 180

Query: 181 ASSSAEELGGAKVVAMALIHPFFGDGGENRLWKYLCSET-----KLLRPTIEDLAKLGCK 240
           A  +     G ++  MAL+HP+FG   ++++W Y+C E        L+P  EDLA++GC+
Sbjct: 181 AGWAGLP-AGVRIAGMALVHPYFGGTDDDKMWLYMCPENGGLADPRLKPPPEDLARVGCE 240

Query: 241 RVKIFLAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVFHLKKPECEKAVDLLEK 300
           +V +F+A+ D L+  G  Y E+LK SGW G+VE VEH  E+HVFH+ KP+C+KA+DL +K
Sbjct: 241 KVLVFVAQKDHLRGRGLWYREELKRSGWGGSVEVVEHEGESHVFHILKPQCDKALDLKDK 300

[Close]

BLAST of Cucsa.085230.1 vs. TrEMBL
Match: F6HQD5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_03s0063g00800 PE=4 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 7.2e-83
Identity = 162/302 (53.64%), Postives = 201/302 (66.56%), Query Frame = 1


Query: 7   KNDD--VAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPAVSAR 66
           ++DD  V FD   F RVY DG VQR      I PAD  D  S  R+KDV +S +  VS R
Sbjct: 2   ESDDAKVVFDC-RFFRVYKDGHVQRHRPIEKIPPAD--DLHSGLRAKDVVVSPETGVSVR 61

Query: 67  VFIPSSADPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRLAPEH 126
           + +P   DP+QKLPLL Y+HGG F  ESAFS ++  ++ SL ++AN + VSVEYRLAPEH
Sbjct: 62  LLLPKIKDPDQKLPLLFYIHGGGFSFESAFSPRFDAYLKSLVSQANVIGVSVEYRLAPEH 121

Query: 127 PIPACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAARASSS 186
           PIPACY+D W AL+WVA+H N +G EPWLN+Y + +R+ +AGDSAGANI H L  R  S 
Sbjct: 122 PIPACYDDSWAALQWVASHANGNGPEPWLNSYANLSRVFIAGDSAGANISHTLMVRVGSL 181

Query: 187 AEELGGAKVVAMALIHPFFGDGGENRLWKYLCS-----ETKLLRPTIEDLAKLGCKRVKI 246
              L GA VV M L+HP+FG   ++ +W Y+C      E   LRPT ED+A LGC RV +
Sbjct: 182 G--LAGANVVGMVLVHPYFGGTTDDGVWLYMCPNNGGLEDPRLRPTAEDMAMLGCGRVLV 241

Query: 247 FLAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVFHLKKPECEKAVDLLEKLASF 302
           FLAEND L+  G NY E+LK SGW G VETVE+  E HVFHL  P CE A  L+ K+ SF
Sbjct: 242 FLAENDHLRDVGWNYCEELKKSGWEGMVETVENHGERHVFHLMNPRCENAATLMGKIVSF 298

[Close]

BLAST of Cucsa.085230.1 vs. TAIR10
Match: AT3G48690.1 (AT3G48690.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 233.8 bits (595), Expect = 1.4e-61
Identity = 125/321 (38.94%), Postives = 186/321 (57.94%), Query Frame = 1


Query: 8   NDDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPAVSARVFI 67
           + ++A D  P L++Y  GR++RLM  + + P+   +P++   SKDV  S D  +S R+++
Sbjct: 2   DSEIAVDCSPLLKIYKSGRIERLMGEATVPPSS--EPQNGVVSKDVVYSADNNLSVRIYL 61

Query: 68  P--SSADPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRLAPEHP 127
           P  ++A+ + KLPLL+Y HGG F IE+AFS  YH  + +  + +N VAVSV+YR APEHP
Sbjct: 62  PEKAAAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHP 121

Query: 128 IPACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAARASSS- 187
           I   ++D W AL+WV  H+   G E WLN + DF+R+ L+GDSAGANI H++A RA+   
Sbjct: 122 ISVPFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEK 181

Query: 188 -AEELGGAKVVAMALIHPFFG--------DGGENRL-------WKYLCSETK-------- 247
            +  L    +  + L+HP+F         D  +  L       W      +K        
Sbjct: 182 LSPGLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLL 241

Query: 248 -LLRPTIEDLAKLGCKRVKIFLAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVF 301
            +++    DL+ LGC +V + +AE D L   G  Y   L+ SGW G VE VE   E+HVF
Sbjct: 242 NVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVF 301

[Close]

BLAST of Cucsa.085230.1 vs. TAIR10
Match: AT1G19190.1 (AT1G19190.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 231.1 bits (588), Expect = 9.1e-61
Identity = 120/314 (38.22%), Postives = 178/314 (56.69%), Query Frame = 1


Query: 8   NDDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPAVSARVFI 67
           + ++AFD+ P  R++ +G ++RL+  + + P+   +P++   SKD   S +  +S R+++
Sbjct: 2   DSEIAFDYSPRFRIFKNGGIERLVPETFVPPSL--NPENGVVSKDAVYSPEKNLSLRIYL 61

Query: 68  PSSA---DPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRLAPEH 127
           P ++      +K+PLL+Y HGG F +E+AFS  YH  + S  +  + +AVSVEYR APEH
Sbjct: 62  PQNSVYETGEKKIPLLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAPEH 121

Query: 128 PIPACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAARASSS 187
           PIP  YED WDA++W+  H+ R G E WLN + DF+++ LAGDSAGANI H++A R    
Sbjct: 122 PIPTLYEDSWDAIQWIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVDKE 181

Query: 188 AEELGGAKVVAMALIHPFFGDGG------------ENRLWKYLCS------ETKLLRPTI 247
                  K+  M L HP+F                  RLW+          E   +    
Sbjct: 182 KLPPENFKISGMILFHPYFLSKALIEEMEVEAMRYYERLWRIASPDSGNGVEDPWINVVG 241

Query: 248 EDLAKLGCKRVKIFLAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVFHLKKPEC 301
            DL  LGC+RV + +A ND L  GG +Y  +L+ SGW G V+ +E  EE HVFHL+ P+ 
Sbjct: 242 SDLTGLGCRRVLVMVAGNDVLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLRDPDS 301

[Close]

BLAST of Cucsa.085230.1 vs. TAIR10
Match: AT1G47480.1 (AT1G47480.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 228.8 bits (582), Expect = 4.5e-60
Identity = 133/320 (41.56%), Postives = 183/320 (57.19%), Query Frame = 1


Query: 4   STNKNDDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPAVSA 63
           ST K   V+ +  P+L V+TDG V+RL  T    P    DP +   SKD+ I     +SA
Sbjct: 3   STKKQ--VSLELLPWLVVHTDGTVERLAGTEVCPPGL--DPITGVFSKDIIIEPKTGLSA 62

Query: 64  RVFIPSSADPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRLAPE 123
           R++ P S  P QK+PL+LY HGGAF I S     YH  +  +  +AN +AVSV YRLAPE
Sbjct: 63  RIYRPFSIQPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPE 122

Query: 124 HPIPACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAARASS 183
           HP+P  YED W AL+ + A      +EPW+N Y D + + L GDSAGANI H+LA RA  
Sbjct: 123 HPLPTAYEDSWTALKNIQAI-----NEPWINDYADLDSLFLVGDSAGANISHHLAFRAKQ 182

Query: 184 SAEELGGAKVVAMALIHPFFGD----GGE----------NRLWKYLC-----SETKLLRP 243
           S + L   K+  + +IHP+F      G E          +  W+++C     S+   + P
Sbjct: 183 SDQTL---KIKGIGMIHPYFWGTQPIGAEIKDEARKQMVDGWWEFVCPSEKGSDDPWINP 242

Query: 244 TIE---DLAKLGCKRVKIFLAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVFHL 302
             +   DL  LGC+RV I +AE D L   GK Y E L  S W G VE +E  E++HVFH+
Sbjct: 243 FADGSPDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHI 302

[Close]

BLAST of Cucsa.085230.1 vs. TAIR10
Match: AT3G48700.1 (AT3G48700.1 carboxyesterase 13)

HSP 1 Score: 223.0 bits (567), Expect = 2.5e-58
Identity = 127/329 (38.60%), Postives = 186/329 (56.53%), Query Frame = 1


Query: 8   NDDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPAVSARVFI 67
           + ++A D+ P L +Y  GR++RL+  + + P+   +P++   SKDV  S D  +S R+++
Sbjct: 2   DSEIAADYSPMLIIYKSGRIERLVGETTVPPSS--NPQNGVVSKDVVYSPDNNLSLRIYL 61

Query: 68  PSSA-----DPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRLAP 127
           P  A     + + KLPLL+Y HGG F +E+AFS  YH  + +  + ++ VAVSV+YR AP
Sbjct: 62  PEKAATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAP 121

Query: 128 EHPIPACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYL---AA 187
           EHPIP  Y+D W AL+WV +H+   GSE WLN + DF+++ LAGDSAGANI H++   AA
Sbjct: 122 EHPIPTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAA 181

Query: 188 RASSSAEELGGAKVVAMALIHPFFG-----DGGE----------NRLWKYLCSETK---- 247
           +   S E L  + +  + L+HP+F      D  E            +W      +K    
Sbjct: 182 KDKLSPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGSD 241

Query: 248 -----LLRPTIEDLAKLGCKRVKIFLAENDFLKSGGKNYEEDLKSSGWNGTV-ETVEHGE 304
                +++    DL+ LGC +V + +AE D L   G  Y E L  S WNG V + VE   
Sbjct: 242 DPFINVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETKG 301

[Close]

BLAST of Cucsa.085230.1 vs. TAIR10
Match: AT1G49660.1 (AT1G49660.1 carboxyesterase 5)

HSP 1 Score: 222.2 bits (565), Expect = 4.2e-58
Identity = 123/318 (38.68%), Postives = 177/318 (55.66%), Query Frame = 1


Query: 10  DVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPAVSARVFIPS 69
           ++A +F PF R+Y DGRV+RL+ T D +PA  D P     SKDV  S +  +S R+F+P 
Sbjct: 4   EIASEFLPFCRIYKDGRVERLIGT-DTIPASLD-PTYDVVSKDVIYSPENNLSVRLFLPH 63

Query: 70  SADP---NQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRLAPEHPI 129
            +       KLPLL+Y+HGGA+ IES FS  YH ++  +   AN +AVSV+YR APE P+
Sbjct: 64  KSTKLTAGNKLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDPV 123

Query: 130 PACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAARASSSAE 189
           PA YED W A++W+ AH N  G   W+N + DF ++ L GDSAG NI H++A +A    E
Sbjct: 124 PAAYEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGK--E 183

Query: 190 ELGGAKVVAMALIHP-FFGD--------------GGENRLWKYLCSETKL---------L 249
           +    K+  +A++HP F+G                G   +W+ + S   +         +
Sbjct: 184 KKLDLKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFNV 243

Query: 250 RPTIEDLAKLGCKRVKIFLAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVFHLK 301
             +  D + LGC +V + +A  D     G  Y   L+   W GTVE VE   E+HVFHL+
Sbjct: 244 NGSGSDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQ 303

[Close]

BLAST of Cucsa.085230.1 vs. NCBI nr
Match: gi|778669131|ref|XP_004145358.2| (PREDICTED: 2-hydroxyisoflavanone dehydratase-like [Cucumis sativus])

HSP 1 Score: 623.2 bits (1606), Expect = 2.3e-175
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 1


Query: 1   MASSTNKNDDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPA 60
           MASSTNKNDDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPA
Sbjct: 1   MASSTNKNDDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPA 60

Query: 61  VSARVFIPSSADPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRL 120
           VSARVFIPSSADPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRL
Sbjct: 61  VSARVFIPSSADPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRL 120

Query: 121 APEHPIPACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAAR 180
           APEHPIPACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAAR
Sbjct: 121 APEHPIPACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAAR 180

Query: 181 ASSSAEELGGAKVVAMALIHPFFGDGGENRLWKYLCSETKLLRPTIEDLAKLGCKRVKIF 240
           ASSSAEELGGAKVVAMALIHPFFGDGGENRLWKYLCSETKLLRPTIEDLAKLGCKRVKIF
Sbjct: 181 ASSSAEELGGAKVVAMALIHPFFGDGGENRLWKYLCSETKLLRPTIEDLAKLGCKRVKIF 240

Query: 241 LAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVFHLKKPECEKAVDLLEKLASFI 300
           LAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVFHLKKPECEKAVDLLEKLASFI
Sbjct: 241 LAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVFHLKKPECEKAVDLLEKLASFI 300

Query: 301 NLD 304
           NLD
Sbjct: 301 NLD 303

[Close]

BLAST of Cucsa.085230.1 vs. NCBI nr
Match: gi|659118613|ref|XP_008459211.1| (PREDICTED: 2-hydroxyisoflavanone dehydratase-like [Cucumis melo])

HSP 1 Score: 572.4 bits (1474), Expect = 4.7e-160
Identity = 275/303 (90.76%), Postives = 290/303 (95.71%), Query Frame = 1


Query: 1   MASSTNKNDDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPA 60
           MASSTNKN++VAFD FPFLRVY+DGRVQRLM TSD VPADADDPKSPFRSKDVTISTDPA
Sbjct: 1   MASSTNKNNEVAFDLFPFLRVYSDGRVQRLMITSDFVPADADDPKSPFRSKDVTISTDPA 60

Query: 61  VSARVFIPSSADPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRL 120
           VSARVFIPSSADPNQKLPLLLYVHGGAFCIESAF+LQYHQHVG+LAA+ANAVAVSVEYRL
Sbjct: 61  VSARVFIPSSADPNQKLPLLLYVHGGAFCIESAFNLQYHQHVGALAAEANAVAVSVEYRL 120

Query: 121 APEHPIPACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAAR 180
           APEHPIPACY+DCWDALRWVAAHVNRDG EPWLNTYVDFNRICLAGDSAGANICHYLAAR
Sbjct: 121 APEHPIPACYDDCWDALRWVAAHVNRDGLEPWLNTYVDFNRICLAGDSAGANICHYLAAR 180

Query: 181 ASSSAEELGGAKVVAMALIHPFFGDGGENRLWKYLCSETKLLRPTIEDLAKLGCKRVKIF 240
           ASSS+EELGGAKVVAMALIHPFFGDG E+RLWKYLCSET+L+RPT E+LAK+GCKRVKIF
Sbjct: 181 ASSSSEELGGAKVVAMALIHPFFGDGREDRLWKYLCSETRLVRPTKEELAKMGCKRVKIF 240

Query: 241 LAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVFHLKKPECEKAVDLLEKLASFI 300
           LAENDFLK GG+NY+EDLK SGWNG VETVEHG ENHVFHLK PE E AVDLLEKLASFI
Sbjct: 241 LAENDFLKFGGRNYDEDLKRSGWNGVVETVEHGGENHVFHLKNPESENAVDLLEKLASFI 300

Query: 301 NLD 304
           NLD
Sbjct: 301 NLD 303

[Close]

BLAST of Cucsa.085230.1 vs. NCBI nr
Match: gi|470117383|ref|XP_004294838.1| (PREDICTED: 2-hydroxyisoflavanone dehydratase-like [Fragaria vesca subsp. vesca])

HSP 1 Score: 345.9 bits (886), Expect = 7.2e-92
Identity = 168/299 (56.19%), Postives = 211/299 (70.57%), Query Frame = 1


Query: 9   DDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPAVSARVFIP 68
           DDV   FFPF +VY DGRV+R M +    P D  DP S  RS DV IST+P VSARVF+P
Sbjct: 10  DDVEHQFFPFFKVYKDGRVERFMPSEKQPPCD--DPSSTVRSNDVIISTEPVVSARVFMP 69

Query: 69  SSAD-PNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRLAPEHPIP 128
              + P +KLP+++Y+HGG F I SAF   YH++V SL A+AN +AVSV+YRLAPEHPIP
Sbjct: 70  RLPNNPEKKLPVVVYIHGGGFSIASAFHPGYHKYVSSLVAQANVIAVSVDYRLAPEHPIP 129

Query: 129 ACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAARASSSAEE 188
           ACYED W  L WVA+H N DG +PWLN++ DF R+ LAGDSAGANI H + +R  S+   
Sbjct: 130 ACYEDSWAVLNWVASHANGDGPDPWLNSHADFGRVFLAGDSAGANISHNIVSRVGSTG-- 189

Query: 189 LGGAKVVAMALIHPFFGDGGENRLWKYLCSET-----KLLRPTIEDLAKLGCKRVKIFLA 248
           L G K+V +AL+HP+FG   ++++W Y+C E        L+P +EDL +LGC+RV +F+A
Sbjct: 190 LPGVKIVGVALVHPYFGGTEDDKMWLYMCPENGGLDDPRLKPAVEDLRRLGCERVLVFVA 249

Query: 249 ENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVFHLKKPECEKAVDLLEKLASFIN 302
             D L+  G +Y E LK SGW GTVE VEH EENHVFHL KPE E AVDL+ +L SFIN
Sbjct: 250 GKDHLRERGTSYVEALKGSGWKGTVEIVEHEEENHVFHLMKPESENAVDLMSRLVSFIN 304

[Close]

BLAST of Cucsa.085230.1 vs. NCBI nr
Match: gi|158564570|gb|ABW74473.1| (CXE carboxylesterase [Paeonia suffruticosa])

HSP 1 Score: 333.2 bits (853), Expect = 4.8e-88
Identity = 162/301 (53.82%), Postives = 209/301 (69.44%), Query Frame = 1


Query: 5   TNKNDDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPAVSAR 64
           T   DDVA +FFPF RVY DGRV+R M T  + P D  DP +  RSKDV IS  P V+ R
Sbjct: 29  TPPGDDVAHEFFPFFRVYKDGRVERFMPTEKVPPTD--DPNTGVRSKDVQIS--PEVAVR 88

Query: 65  VFIPSSADPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRLAPEH 124
           +F+P   DP QK+P+L Y HGG F I SAF+  YH +V SL A+AN +AVSV+YRLAPEH
Sbjct: 89  IFLPKIDDPTQKVPVLFYTHGGGFSIGSAFAQGYHNYVSSLVAEANVIAVSVDYRLAPEH 148

Query: 125 PIPACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAARASSS 184
           PIPACYED W+A +WVA+H N +G EPWLN + DF R+ + GDSAGANI H LAAR  S+
Sbjct: 149 PIPACYEDSWEAFKWVASHANGNGPEPWLNDHADFRRVFMTGDSAGANITHTLAARIGST 208

Query: 185 AEELGGAKVVAMALIHPFFGDGGENRLWKYLCS-----ETKLLRPTIEDLAKLGCKRVKI 244
             EL G KV+ +AL+HP+FG   ++++W +LC      E   L+P  EDLAKLGC+++ I
Sbjct: 209 --ELPGVKVIGIALVHPYFGGTDDDKMWLFLCPTNGGLEDPRLKPATEDLAKLGCEKMLI 268

Query: 245 FLAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVFHLKKPECEKAVDLLEKLASF 301
           F+A+ D LK  G +Y ++LK SGW GTVE  E+  ++HVFHL  P C+ A  + ++L SF
Sbjct: 269 FVADEDHLKERGISYYDELKKSGWKGTVEIEENKGQHHVFHLMNPTCDDAKAMKKRLVSF 323

[Close]

BLAST of Cucsa.085230.1 vs. NCBI nr
Match: gi|225428751|ref|XP_002285042.1| (PREDICTED: probable carboxylesterase 5 [Vitis vinifera])

HSP 1 Score: 322.8 bits (826), Expect = 6.5e-85
Identity = 158/305 (51.80%), Postives = 208/305 (68.20%), Query Frame = 1


Query: 2   ASSTNKNDDVAFDFFPFLRVYTDGRVQRLMTTSDIVPADADDPKSPFRSKDVTISTDPAV 61
           A     N  ++  FFPF ++Y DGRV+R M T  + P+D  DP +  RSKDV IS +  V
Sbjct: 11  AKLEKSNGQLSHLFFPFFKIYQDGRVERFMHTDHVPPSD--DPLTGVRSKDVIISPETGV 70

Query: 62  SARVFIPSSADPNQKLPLLLYVHGGAFCIESAFSLQYHQHVGSLAAKANAVAVSVEYRLA 121
           SAR+FIP   +PN KLPLL+Y+HGG F I+SAFS  Y+ +V SL A+AN +A+SV+YRLA
Sbjct: 71  SARLFIPKLPNPNCKLPLLIYIHGGGFSIQSAFSTSYNHYVKSLVAEANVIALSVDYRLA 130

Query: 122 PEHPIPACYEDCWDALRWVAAHVNRDGSEPWLNTYVDFNRICLAGDSAGANICHYLAARA 181
           PEHPIPACY+D W A++W A+H N DG + WLN + DF+R+  AGDSAG NI + LA R 
Sbjct: 131 PEHPIPACYDDSWAAVQWAASHANGDGPDTWLNNHADFSRVFFAGDSAGGNISNTLAFRV 190

Query: 182 SSSAEELGGAKVVAMALIHPFFGDGGENRLWKYLCS-----ETKLLRPTIEDLAKLGCKR 241
            SS   L G KVV + L+HP+FG  G++++W Y+C      E   L+P  EDLA+LGC+R
Sbjct: 191 GSSG--LPGVKVVGVVLVHPYFGGTGDDQMWLYMCPNHGGLEDPRLKPGAEDLARLGCER 250

Query: 242 VKIFLAENDFLKSGGKNYEEDLKSSGWNGTVETVEHGEENHVFHLKKPECEKAVDLLEKL 301
           V +F+AE D L+    +Y E LK S W GTVE VE+  E HVFHL  P+CE A  L++K+
Sbjct: 251 VLMFVAEKDHLRPVAWDYYEKLKKSEWKGTVEIVENHGEEHVFHLMNPKCENAAVLMKKI 310

The following BLAST results are available for this feature:

BLAST of Cucsa.085230.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
HIDM_GLYEC	1.9e-60	38.04	2-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata GN=HIDM PE=1 SV=1	[more]
CXE12_ARATH	2.5e-60	38.94	Probable carboxylesterase 12 OS=Arabidopsis thaliana GN=CXE12 PE=1 SV=1	[more]
CXE1_ARATH	1.6e-59	38.22	Probable carboxylesterase 1 OS=Arabidopsis thaliana GN=CXE1 PE=2 SV=1	[more]
CXE2_ARATH	8.0e-59	41.56	Probable carboxylesterase 2 OS=Arabidopsis thaliana GN=CXE2 PE=2 SV=1	[more]
HIDH_SOYBN	1.2e-57	36.91	2-hydroxyisoflavanone dehydratase OS=Glycine max GN=HIDH PE=1 SV=1	[more]

BLAST of Cucsa.085230.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LNZ1_CUCSA	1.6e-175	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G234530 PE=4 SV=1	[more]
A8TU55_PAESU	3.4e-88	53.82	CXE carboxylesterase OS=Paeonia suffruticosa PE=2 SV=1	[more]
F6HQD6_VITVI	4.5e-85	51.80	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_03s0063g00810 PE=4 SV=...	[more]
A0A059BCP2_EUCGR	1.3e-84	49.84	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_G01495 PE=4 SV=1	[more]
F6HQD5_VITVI	7.2e-83	53.64	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_03s0063g00800 PE=4 SV=...	[more]

BLAST of Cucsa.085230.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G48690.1	1.4e-61	38.94	alpha/beta-Hydrolases superfamily protein	[more]
AT1G19190.1	9.1e-61	38.22	alpha/beta-Hydrolases superfamily protein	[more]
AT1G47480.1	4.5e-60	41.56	alpha/beta-Hydrolases superfamily protein	[more]
AT3G48700.1	2.5e-58	38.60	carboxyesterase 13	[more]
AT1G49660.1	4.2e-58	38.68	carboxyesterase 5	[more]

BLAST of Cucsa.085230.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778669131\|ref\|XP_004145358.2\|	2.3e-175	100.00	PREDICTED: 2-hydroxyisoflavanone dehydratase-like [Cucumis sativus]	[more]
gi\|659118613\|ref\|XP_008459211.1\|	4.7e-160	90.76	PREDICTED: 2-hydroxyisoflavanone dehydratase-like [Cucumis melo]	[more]
gi\|470117383\|ref\|XP_004294838.1\|	7.2e-92	56.19	PREDICTED: 2-hydroxyisoflavanone dehydratase-like [Fragaria vesca subsp. vesca]	[more]
gi\|158564570\|gb\|ABW74473.1\|	4.8e-88	53.82	CXE carboxylesterase [Paeonia suffruticosa]	[more]
gi\|225428751\|ref\|XP_002285042.1\|	6.5e-85	51.80	PREDICTED: probable carboxylesterase 5 [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR013094	AB_hydrolase_3

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016787	hydrolase activity

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016787	hydrolase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsa.085230	Cucsa.085230	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsa.085230.1	Cucsa.085230.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.085230.1.CDS.2	Cucsa.085230.1.CDS.2	CDS
Cucsa.085230.1.CDS.1	Cucsa.085230.1.CDS.1	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.085230.1.three_prime_UTR.1	Cucsa.085230.1.three_prime_UTR.1	three_prime_UTR

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.085230.1.five_prime_UTR.1	Cucsa.085230.1.five_prime_UTR.1	five_prime_UTR

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013094	Alpha/beta hydrolase fold-3	PFAM	PF07859	Abhydrolase_3	coord: 80..202 score: 2.3
None	No IPR available	PANTHER	PTHR23024	MEMBER OF 'GDXG' FAMILY OF LIPOLYTIC ENZYMES	coord: 8..303 score: 5.4E
None	No IPR available	PANTHER	PTHR23024:SF228	SUBFAMILY NOT NAMED	coord: 8..303 score: 5.4E