Cucsa.081160.3 (mRNA) Cucumber (Gy14) v1

NameCucsa.081160.3
TypemRNA
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionU4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein
Locationscaffold00817 : 9772 .. 12368 (-)
Sequence length995
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTGCTTCACTCTGAGCTTACGGAATCGGAAATTCTTACAAATCCGCTATGGTCGACCGTGAACGAGACAGAGATAGAGGCCGCGACAGAGATAAAGATCGGGACCGTGATAGATACAAAGACCGGGAGCGAGACCGTGACCATCGGGAACGTGACCGGCGTAGGGATCGAGAAGATCGTGACCACCGTGAACGTGATCGGGACCGGGAACGCCGTAGGGATCAGGACGATCGTGACCGTGAAAGGGAGCGTGGCCGGAGTAAAAGGTCACTCACTCCCGACCGCTCAAGATCCCGCCGCTCTCGCACCCGCTCGCCGGACCGGCACCGGTCGCGGTCTCTTTCTCGTACTCCCGAGGACCGGCCATCTCGCCGCCGCCATCGTTCTCCTTCCCCAGATCATGCTCGGAAGCGTCATCGCCGGGATTTGACTGCGGATGACGAAAAGGAGCGGCAGAAAGCAGTTTCTGACTTTGTTGATGGCATTGCGAAAGAGCAGCACAAGCAGAAGAATAATAAAGACAACGTTGGTGGCGGAGAAGTAGTGGAGGAGGCCATGGCTGATGAAGATGAAATGGAGATGATGAAGAAATTAGGTATTCCCGTGGGTTTCGATTCGACCAAGGGCAAACCTGTCCCAGGTGCTGATGTGAGTGGCGTTAGGGTAGTTACCAAGAGGCAGCCTAGGCAGTACATGAATCGTCGTGGAGGGTTCAACAGGCCGTTGCCGGCCGAGCGCAGCCGGTAGCTAATATATTTGGCTAGGTCACTGAGGTTAGTCTATTGAACTGTTATTGGGATTTCTTAGCTCCGCCGTATTAAACTGTTGCAACAATTGAATAGCTTAGTCCACTGCCTTCTTCCTAAGGTTTGAGCAACAACTGCAACAAGAGTGTTTTTTGTATTTGTACAAAGATTAGAATGCTGTCTTCTTCCTGACATTCGGTTTTAGTAGTCCGATTAATGTTAATTCATTGTGCACTGTGTTTTTATGCTCCAGTTCCTAGTCACTTATAGTATTCTAGATGTTCAATGGGGTAGATGAGTATCTCGTCATTGTTGAGGATCTGACTAAAGTATTTGACATCTTTACTCCTGAAAATGCTCCTTAGAGAGAACACTAATTTCTTTGTCAATTTCATCTGTTTAAGATGGGTTTTAGACTAATGTTACCAGTAGTTAGTAGTTACTTAACGTTAGAGGTATGAACTTTTCACACATTTCTAAATATCACCAAACAGTTAGAACTTCATTGCCACCATTTTTTTTGTACAATGGTTGAAGGTCATACTCTGCCTAGTATTATCAAAGAGAACTTGAAGGACCCAAAATTTTGTTGAACGTATTTTTGGCATTTAGTTTTTGAAGTTATAATTGTAAAATTCTTAATCCAGTTGAGGAAACGTTGTGATGTACTTCTTCGGATGTGTTATAGGGCCGGGGCTTTTTGTATCAAAATATTGAGCTTATGTGTATGGTAGCTGGGCATGCGAATTTCTTGTGTGAGATAGTTTGTTTATGTTATTTGAGCCAACAGTTGATGAAAGTTCACAAGTGATGAATTTAGGAATATATTCAGACTTGCTGATATATGAAAATATGATTTAGTTTTTCATTAAAACAAAGAAAAGATTTAGGAGCTGTTGCCTGAAACTTACAAAGATGGTAAAATAATGAAACTGAGTATAGCTCTCCCGTGCTTCAGTGTAGCAACCAAGACTGACATTCTTCCTGAGATATCATTGAGCGGTGGGAGATAAATGCAGACCAGGCTATTGCAATGTGCTCGCATATTCTGGTAATAAAATCTTGTTACTGAATGTGTTTCCTGCTTAACCTGAAGTTTTGATATAGCTTGATTCGGATTTCAAATCCTCCCCTATCTTAGGGGACGCCTTGTGATTGTGAGTTCTTAGGGGTCCGCCTTGTGATTGTGAGTTCCAAAGACTTCCATCTGGTTTCTGAAATTAAAACTCACCCATATAGTTTGTGAGTGATTCAAGAGTTTGTGTGTAGATCCTTTAAGCACCCATTTAAACATAATCCTTTTTAGTTGCCAATTGCAAACACTCCTGCAATAACCTTTAGGTGCTTTGAAGTTTTCCGTTGATTTCATTTATCAATGAAATGTTTCTTTATCCCGAAAAAAAGTAACTAACAGCATAAACATCTGTGTGGGTGTCTGTTTATCGTCGCATTCTTCTAGATTGAAGTTTATTCTAGTATTTTATCAGTGCTGGGTTCCTTATAATAAAAAGTTATTTTTATGTACTCTTTATTTCATGTCAAGCTAGTTCCTTTCTTTTATTTAGTAATATTATCATTTACTCATGTCCGTTCAATGACACTAATTGAACCTGTTTTTGGTTTCTGCAGGATTTTGGTTTGCTTGACTTTGCTGGAGAGGCCAGATGGAAGTCTTTGGGCACAGTATTGTTTGTATGTAATATATATTCATGAAACCTTAGAATTCGAGTCGAGTTGAGTTAATTGATACTCGAAGAACGAGGGATATTTTTGGCTATTGTAGAAATTGGAGAATTTTCTATGTTGCTTATTTGTTTTCAGTAAAAAATGGATAGTGCTTAGATCAATTG

mRNA sequence

GTTTGCTTCACTCTGAGCTTACGGAATCGGAAATTCTTACAAATCCGCTATGGTCGACCGTGAACgagacagagatagaggccgcgacagagataaagatcgggaccgtgatagatacaaagaccgggagcgagaccgtgacCATCGGGAACGTGACCGGCGTAGGGATCGAGAAGATCGTGACCACCGTGAACGTGATCGGGACCGGGAACGCCGTAGGGATCAGGACGATCGTGACCGTGAAAGGGAGCGTGGCCGGAGTAAAAGGTCACTCACTCCCGACCGCTCAAGATCCCGCCGCTCTCGCACCCGCTCGCCGGACCGGCACCGGTCGCGGTCTCTTTCTCGTACTCCCGAGGACCGGCCATCTCGCCGCCGCCATCGTTCTCCTTCCCCAGATCATGCTCGGAAGCGTCATCGCCGGGATTTGACTGCGGATGACGAAAAGGAGCGGCAGAAAGCAGTTTCTGACTTTGTTGATGGCATTGCGAAAGAGCAGCACAAGCAGAAGAATAATAAAGACAACGTTGGTGGCGGAGAAGTAGTGGAGGAGGCCATGGCTGATGAAGATGAAATGGAGATGATGAAGAAATTAGGTATTCCCGTGGGTTTCGATTCGACCAAGGGCAAACCTGTCCCAGGTGCTGATGTGAGTGGCGTTAGGGTAGTTACCAAGAGGCAGCCTAGGCAGTACATGAATCGTCGTGGAGGGTTCAACAGGCCGTTGCCGGCCGAGCGCAGCCGGTAGCTAATATATTTGGCTAGGTCACTGAGGATTTTGGTTTGCTTGACTTTGCTGGAGAGGCCAGATGGAAGTCTTTGGGCACAGTATTGTTTGTATGTAATATATATTCATGAAACCTTAGAATTCGAGTCGAGTTGAGTTAATTGATACTCGAAGAACGAGGGATATTTTTGGCTATTGTAGAAATTGGAGAATTTTCTATGTTGCTTATTTGTTTTCAGTAAAAAATGGATAGTGCTTAGATCAATTG

Coding sequence (CDS)

ATGGTCGACCGTGAACGAGACAGAGATAGAGGCCGCGACAGAGATAAAGATCGGGACCGTGATAGATACAAAGACCGGGAGCGAGACCGTGACCATCGGGAACGTGACCGGCGTAGGGATCGAGAAGATCGTGACCACCGTGAACGTGATCGGGACCGGGAACGCCGTAGGGATCAGGACGATCGTGACCGTGAAAGGGAGCGTGGCCGGAGTAAAAGGTCACTCACTCCCGACCGCTCAAGATCCCGCCGCTCTCGCACCCGCTCGCCGGACCGGCACCGGTCGCGGTCTCTTTCTCGTACTCCCGAGGACCGGCCATCTCGCCGCCGCCATCGTTCTCCTTCCCCAGATCATGCTCGGAAGCGTCATCGCCGGGATTTGACTGCGGATGACGAAAAGGAGCGGCAGAAAGCAGTTTCTGACTTTGTTGATGGCATTGCGAAAGAGCAGCACAAGCAGAAGAATAATAAAGACAACGTTGGTGGCGGAGAAGTAGTGGAGGAGGCCATGGCTGATGAAGATGAAATGGAGATGATGAAGAAATTAGGTATTCCCGTGGGTTTCGATTCGACCAAGGGCAAACCTGTCCCAGGTGCTGATGTGAGTGGCGTTAGGGTAGTTACCAAGAGGCAGCCTAGGCAGTACATGAATCGTCGTGGAGGGTTCAACAGGCCGTTGCCGGCCGAGCGCAGCCGGTAG

Protein sequence

MVDRERDRDRGRDRDKDRDRDRYKDRERDRDHRERDRRRDREDRDHRERDRDRERRRDQDDRDRERERGRSKRSLTPDRSRSRRSRTRSPDRHRSRSLSRTPEDRPSRRRHRSPSPDHARKRHRRDLTADDEKERQKAVSDFVDGIAKEQHKQKNNKDNVGGGEVVEEAMADEDEMEMMKKLGIPVGFDSTKGKPVPGADVSGVRVVTKRQPRQYMNRRGGFNRPLPAERSR*
BLAST of Cucsa.081160.3 vs. Swiss-Prot
Match: SNR27_MOUSE (U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein OS=Mus musculus GN=Snrnp27 PE=1 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 6.4e-24
Identity = 81/195 (41.54%), Postives = 100/195 (51.28%), Query Frame = 1

Query: 33  RERDRRRDREDRDHRERDRDRERRRDQDDRDRERERGRSKRSLTPDRSRSRRSRTRSPDR 92
           R R R   RE R  R   RDRERRR +  R RER+R              RRSR+RSP R
Sbjct: 3   RSRSRSPRRERRRSRSTSRDRERRRRERSRSRERDR--------------RRSRSRSPHR 62

Query: 93  HRSRSLSRTPEDRPSRRRHRSPSPDHARKRHRRDLTADDEKERQKAVSDFVDGIAKEQHK 152
            RSRS           RRHRS SP  +R + RRD    +EK+  K +        K + +
Sbjct: 63  RRSRS----------PRRHRSTSPSPSRLKERRD----EEKKETKEI--------KNKER 122

Query: 153 QKNNKDNVGGGEVVEEAMADEDEMEMMKKLGIPVGFDSTKGKPVPGA-DVSGVRVVTKRQ 212
           Q   +D  G           E+E+EMMK +G    FDSTKGK V G+ +   + V  KR+
Sbjct: 123 QITEEDLEG---------KTEEEIEMMKLMGF-ASFDSTKGKKVDGSVNAYAINVSQKRK 151

Query: 213 PRQYMNRRGGFNRPL 227
            RQYMNR+GGFNRPL
Sbjct: 183 YRQYMNRKGGFNRPL 151


HSP 2 Score: 47.8 bits (112), Expect = 1.9e-04
Identity = 33/85 (38.82%), Postives = 40/85 (47.06%), Query Frame = 1

Query: 4  RERDRDRGRDRDKDRDRDRYKDRERDRDHRERDRRRDREDRDHRERDRD-RERRRDQDDR 63
          R   R+R R R   RDR+R + RER R  RERDRRR R    HR R R  R  R      
Sbjct: 7  RSPRRERRRSRSTSRDRERRR-RERSRS-RERDRRRSRSRSPHRRRSRSPRRHRSTSPSP 66

Query: 64 DRERERGRSKRSLTPDRSRSRRSRT 88
           R +ER   ++  T +     R  T
Sbjct: 67 SRLKERRDEEKKETKEIKNKERQIT 89

BLAST of Cucsa.081160.3 vs. Swiss-Prot
Match: FRAY2_DICDI (Serine/threonine-protein kinase fray2 OS=Dictyostelium discoideum GN=fray2 PE=3 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 4.2e-23
Identity = 68/143 (47.55%), Postives = 82/143 (57.34%), Query Frame = 1

Query: 3   DRERDRDRGRDRDKDRDRDRYKDRERDRDH-RERDRRRDREDRDHRERDRDRERRRDQD- 62
           DR+R+R+R RDRD++RDRDR +DR RDR   RERDR RDR     R RDR R+R R++D 
Sbjct: 769 DRDRERERERDRDRERDRDRSRDRSRDRSRDRERDRSRDRSRDRDRSRDRSRDRSRERDR 828

Query: 63  DRDRERERGRSKRSLTPDRSRSRRSRTRSPDRHRSRSLSRTPEDRPSRRRHRSPSPDHAR 122
           DR R+R R RS       RSR   SR RS DR RS S  R    R SR + R  S D  R
Sbjct: 829 DRSRDRSRDRS-------RSRDSDSRDRSRDRSRSHSNRRRSRSRDSRNKSRDRSSDSDR 888

Query: 123 KRHR---RDLTADDEKERQKAVS 141
            R R   RD  +   K+  +  S
Sbjct: 889 SRDRSRDRDYKSSKYKKSSRGTS 904


HSP 2 Score: 101.3 bits (251), Expect = 1.5e-20
Identity = 73/181 (40.33%), Postives = 97/181 (53.59%), Query Frame = 1

Query: 8   RDRGRDRDKDRDRDRYK-----------DRERDRDHRERDRRRDREDRDHRERDRDRERR 67
           RDR R+RD+DR    YK           +RERDR +R RDR R+RE    R+R+RDR+R 
Sbjct: 730 RDRERERDRDRSNKTYKNSRSRNVSRDRERERDRHNRSRDRDRERERERDRDRERDRDRS 789

Query: 68  RDQD-DRDRERERGRSK-RSLTPDRSRSR-RSRTRSPDRHRSRSLSR---TPEDRPSRRR 127
           RD+  DR R+RER RS+ RS   DRSR R R R+R  DR RSR  SR      D  SR R
Sbjct: 790 RDRSRDRSRDRERDRSRDRSRDRDRSRDRSRDRSRERDRDRSRDRSRDRSRSRDSDSRDR 849

Query: 128 HRSPSPDHARKRH---------RRDLTADDEKERQKAVS-DFVDGIAKEQHK----QKNN 158
            R  S  H+ +R           RD ++D ++ R ++   D+     K+  +    +KNN
Sbjct: 850 SRDRSRSHSNRRRSRSRDSRNKSRDRSSDSDRSRDRSRDRDYKSSKYKKSSRGTSGRKNN 909


HSP 3 Score: 95.1 bits (235), Expect = 1.1e-18
Identity = 62/168 (36.90%), Postives = 88/168 (52.38%), Query Frame = 1

Query: 8   RDRGRDRDKDRDRDRYKDRERDRDHRERDRRRDREDRDHRERDRDRERRRDQD-DRDRER 67
           RDR R+RD+DR    YK+       R+R+R RDR +R  R+RDR+RER RD+D +RDR+R
Sbjct: 730 RDRERERDRDRSNKTYKNSRSRNVSRDRERERDRHNRS-RDRDRERERERDRDRERDRDR 789

Query: 68  ERGRSKRSLTPDRSRSRRSRTRSPDRHRSRSLSRTPEDRPSRRRHRSPSPDHARKRHRRD 127
            R RS+     DRSR R  R RS DR R R  SR      SR R R  S D +R R R  
Sbjct: 790 SRDRSR-----DRSRDRE-RDRSRDRSRDRDRSRDRSRDRSRERDRDRSRDRSRDRSRSR 849

Query: 128 LTADDEKERQKAVSDFVDGIAKEQHKQKNNKDNVGGGEVVEEAMADED 175
            +   ++ R ++ S      ++ +  +  ++D     +   +   D D
Sbjct: 850 DSDSRDRSRDRSRSHSNRRRSRSRDSRNKSRDRSSDSDRSRDRSRDRD 890


HSP 4 Score: 95.1 bits (235), Expect = 1.1e-18
Identity = 69/177 (38.98%), Postives = 86/177 (48.59%), Query Frame = 1

Query: 3   DRERDRDRGRDRDKDRDRDRYKDRERDRDHRERDRRRDRE---DRDH-RERDRDRERRRD 62
           DR+R RDR RDR +DR+RDR +DR RDRD R RDR RDR    DRD  R+R RDR R RD
Sbjct: 785 DRDRSRDRSRDRSRDRERDRSRDRSRDRD-RSRDRSRDRSRERDRDRSRDRSRDRSRSRD 844

Query: 63  QDDRDR---------ERERGRSKRSLTPDRSRSRRSRTRSPDRHRSRSLSRTPEDRPSRR 122
            D RDR          R R RS       R    +SR RS D  RSR  SR   D  S +
Sbjct: 845 SDSRDRSRDRSRSHSNRRRSRS-------RDSRNKSRDRSSDSDRSRDRSR-DRDYKSSK 904

Query: 123 RHRSPSPDHARKRHRRDLTADDEKERQKAVSDFVDGIAKEQHKQKNNKDNVGGGEVV 167
             +S      RK ++     D +      + D +  +      Q+ N  N   G+V+
Sbjct: 905 YKKSSRGTSGRKNNKIQSKLDSQATHISYLEDKISTLT--NWLQQQNLLNTSNGQVL 950


HSP 5 Score: 56.2 bits (134), Expect = 5.5e-07
Identity = 40/154 (25.97%), Postives = 64/154 (41.56%), Query Frame = 1

Query: 3   DRERDRDRGRDRDKDRDRDRYKDRERDRDHR-------ERDRRRDREDRDHRERDRDRER 62
           D+E +     ++ K R   R        D         E   R + + + ++++      
Sbjct: 666 DKENNSTSKSNKSKSRQSSRASSLSESSDSTSHTSSSDEHSSRYESDRKSYKKKKSSSSS 725

Query: 63  RRDQDDRDRERERGRS--------KRSLTPDRSRSRRSRTRSPDRHRSRSLSRTPEDRPS 122
              + DR+RER+R RS         R+++ DR R R    RS DR R R   R  +    
Sbjct: 726 SNSKRDRERERDRDRSNKTYKNSRSRNVSRDRERERDRHNRSRDRDRERERERDRDRERD 785

Query: 123 RRRHRSPSPDHARKRHR---RDLTADDEKERQKA 139
           R R R  S D +R R R   RD + D ++ R ++
Sbjct: 786 RDRSRDRSRDRSRDRERDRSRDRSRDRDRSRDRS 819

BLAST of Cucsa.081160.3 vs. Swiss-Prot
Match: SNR27_HUMAN (U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein OS=Homo sapiens GN=SNRNP27 PE=1 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 4.2e-23
Identity = 79/195 (40.51%), Postives = 97/195 (49.74%), Query Frame = 1

Query: 33  RERDRRRDREDRDHRERDRDRERRRDQDDRDRERERGRSKRSLTPDRSRSRRSRTRSPDR 92
           R R R   RE R  R   R+RERRR +  R RER+R              RRSR+RSP R
Sbjct: 3   RSRSRSPRRERRRSRSTSRERERRRRERSRSRERDR--------------RRSRSRSPHR 62

Query: 93  HRSRSLSRTPEDRPSRRRHRSPSPDHARKRHRRDLTADDEKERQKAVSDFVDGIAKEQHK 152
            RSRS           RRHRS SP  +R + RRD    + KE             K + +
Sbjct: 63  RRSRS----------PRRHRSTSPSPSRLKERRDEEKKETKE------------TKSKER 122

Query: 153 QKNNKDNVGGGEVVEEAMADEDEMEMMKKLGIPVGFDSTKGKPVPGA-DVSGVRVVTKRQ 212
           Q   +D  G           E+E+EMMK +G    FDSTKGK V G+ +   + V  KR+
Sbjct: 123 QITEEDLEG---------KTEEEIEMMKLMGF-ASFDSTKGKKVDGSVNAYAINVSQKRK 151

Query: 213 PRQYMNRRGGFNRPL 227
            RQYMNR+GGFNRPL
Sbjct: 183 YRQYMNRKGGFNRPL 151


HSP 2 Score: 47.0 bits (110), Expect = 3.3e-04
Identity = 33/84 (39.29%), Postives = 42/84 (50.00%), Query Frame = 1

Query: 4  RERDRDRGRDRDKDRDRDRYKDRERDRDHRERDRRRDREDRDHRERDRDRERRRD---QD 63
          R   R+R R R   R+R+R + RER R  RERDRRR R    HR R R   R R      
Sbjct: 7  RSPRRERRRSRSTSRERERRR-RERSRS-RERDRRRSRSRSPHRRRSRSPRRHRSTSPSP 66

Query: 64 DRDRERERGRSKRSLTPDRSRSRR 85
           R +ER R   K+     +S+ R+
Sbjct: 67 SRLKER-RDEEKKETKETKSKERQ 87

BLAST of Cucsa.081160.3 vs. Swiss-Prot
Match: SNR27_XENLA (U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein OS=Xenopus laevis GN=snrnp27 PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 8.7e-21
Identity = 76/197 (38.58%), Postives = 97/197 (49.24%), Query Frame = 1

Query: 37  RRRDREDRDHRERDRDRERRRDQDDRDRERERGRSKRSLTPDRSRSRRSRTRSPDRHRSR 96
           R R R     RER R R   R+++ R RER R R +R          RSR+RSP R RSR
Sbjct: 3   RSRSRSPERRRERRRSRSASRERERRRRERSRSRERR----------RSRSRSPHRRRSR 62

Query: 97  SLSRTPEDRPSRRRHRSPSPDHARKRHRRDLTADDEKERQKAVSDFVDGIAKEQHKQKNN 156
           S           RRHRS S   +R + RRD   DD+KE                      
Sbjct: 63  S----------PRRHRSSSISPSRLKDRRD---DDKKEP--------------------- 122

Query: 157 KDNVGGGEVVEEAMAD------EDEMEMMKKLGIPVGFDSTKGKPVPGA-DVSGVRVVTK 216
           K++ GGG    +  A+      E+E+EMMK +G    FDS+KGK   G+ +   + V  K
Sbjct: 123 KESKGGGSKERQLAAEDLEGKTEEEIEMMKLMGF-ASFDSSKGKKTDGSVNAYAINVSQK 154

Query: 217 RQPRQYMNRRGGFNRPL 227
           R+ RQYMNR+GGFNRPL
Sbjct: 183 RKYRQYMNRKGGFNRPL 154


HSP 2 Score: 45.8 bits (107), Expect = 7.4e-04
Identity = 29/87 (33.33%), Postives = 37/87 (42.53%), Query Frame = 1

Query: 6  RDRDRGRDRDKDRDRDRYKDRERDRDHRE----RDRRRDREDRDHRERDRDRERRRDQDD 65
          R R R  +R ++R R R   RER+R  RE    R+RRR R    HR R R   R R    
Sbjct: 3  RSRSRSPERRRERRRSRSASRERERRRRERSRSRERRRSRSRSPHRRRSRSPRRHRSSSI 62

Query: 66 RDRERERGRSKRSLTPDRSRSRRSRTR 89
               +  R      P  S+   S+ R
Sbjct: 63 SPSRLKDRRDDDKKEPKESKGGGSKER 89

BLAST of Cucsa.081160.3 vs. Swiss-Prot
Match: RBM25_HUMAN (RNA-binding protein 25 OS=Homo sapiens GN=RBM25 PE=1 SV=3)

HSP 1 Score: 101.7 bits (252), Expect = 1.1e-20
Identity = 70/184 (38.04%), Postives = 105/184 (57.07%), Query Frame = 1

Query: 3   DRERDRDRGRDRDKDRDRDRYKDRERDRD-HRERDRRRDREDRDHRERDRDRERRRDQDD 62
           +RER+R+R R+R ++R+R+R ++RER+++  RER+R RDR+    +ERDRDR+R RD+ D
Sbjct: 311 ERERERERERER-REREREREREREREKEKERERERERDRDRDRTKERDRDRDRERDR-D 370

Query: 63  RDRERERGRSK-RSLTPDRSRSR-RSRTRSPDRHRSRSLSRTPEDRPSRRRHRSPSPDHA 122
           RDRER   R+K RS + ++SR R R R R  +R R R   R  E    R R R    +  
Sbjct: 371 RDRERSSDRNKDRSRSREKSRDREREREREREREREREREREREREREREREREREREKD 430

Query: 123 RKRHRRDLTADDEKERQKAVSDFVDGIAKEQHKQKN--NKDNVGGGEVVEEAMADEDEME 182
           +KR R +   +D  ER+K      +  A  Q + KN   ++     E  +EA  +E+   
Sbjct: 431 KKRDREE-DEEDAYERRKLERKLREKEAAYQERLKNWEIRERKKTREYEKEAEREEERRR 490


HSP 2 Score: 80.9 bits (198), Expect = 2.1e-14
Identity = 56/179 (31.28%), Postives = 96/179 (53.63%), Query Frame = 1

Query: 2   VDRERDRDRGRDRDKDRDRDRYKDRERDRDHRERDRRRDREDRDHRERDRDRERRRDQD- 61
           + + RD  +  + +K +     ++ E++R  RER+R R+RE R+ RER+R+RER R+++ 
Sbjct: 281 ISKFRDTHKKLEEEKGKKEKERQEIEKERRERERERERERERRE-REREREREREREKEK 340

Query: 62  DRDRERERGRSKRSLTPDRSRSR-RSRTRSPDRHRSRSLSRTPEDRPSRRRHRSPSPDHA 121
           +R+RERER R +     DR++ R R R R  DR R R  S       SR R +S   +  
Sbjct: 341 ERERERERDRDR-----DRTKERDRDRDRERDRDRDRERSSDRNKDRSRSREKSRDRERE 400

Query: 122 RKRHR-RDLTADDEKERQKAVSDFVDGIAKEQHKQKNNKDNVGGGEVVEEAMADEDEME 178
           R+R R R+   + E+ER++      +   + + +++  KD     E  EE   +  ++E
Sbjct: 401 RERERERERERERERERER------EREREREREREREKDKKRDREEDEEDAYERRKLE 447

BLAST of Cucsa.081160.3 vs. TrEMBL
Match: A0A0A0LU61_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G423230 PE=4 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 4.2e-131
Identity = 232/232 (100.00%), Postives = 232/232 (100.00%), Query Frame = 1

Query: 1   MVDRERDRDRGRDRDKDRDRDRYKDRERDRDHRERDRRRDREDRDHRERDRDRERRRDQD 60
           MVDRERDRDRGRDRDKDRDRDRYKDRERDRDHRERDRRRDREDRDHRERDRDRERRRDQD
Sbjct: 1   MVDRERDRDRGRDRDKDRDRDRYKDRERDRDHRERDRRRDREDRDHRERDRDRERRRDQD 60

Query: 61  DRDRERERGRSKRSLTPDRSRSRRSRTRSPDRHRSRSLSRTPEDRPSRRRHRSPSPDHAR 120
           DRDRERERGRSKRSLTPDRSRSRRSRTRSPDRHRSRSLSRTPEDRPSRRRHRSPSPDHAR
Sbjct: 61  DRDRERERGRSKRSLTPDRSRSRRSRTRSPDRHRSRSLSRTPEDRPSRRRHRSPSPDHAR 120

Query: 121 KRHRRDLTADDEKERQKAVSDFVDGIAKEQHKQKNNKDNVGGGEVVEEAMADEDEMEMMK 180
           KRHRRDLTADDEKERQKAVSDFVDGIAKEQHKQKNNKDNVGGGEVVEEAMADEDEMEMMK
Sbjct: 121 KRHRRDLTADDEKERQKAVSDFVDGIAKEQHKQKNNKDNVGGGEVVEEAMADEDEMEMMK 180

Query: 181 KLGIPVGFDSTKGKPVPGADVSGVRVVTKRQPRQYMNRRGGFNRPLPAERSR 233
           KLGIPVGFDSTKGKPVPGADVSGVRVVTKRQPRQYMNRRGGFNRPLPAERSR
Sbjct: 181 KLGIPVGFDSTKGKPVPGADVSGVRVVTKRQPRQYMNRRGGFNRPLPAERSR 232

BLAST of Cucsa.081160.3 vs. TrEMBL
Match: A0A0S3R800_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G479700 PE=4 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 3.1e-73
Identity = 156/244 (63.93%), Postives = 185/244 (75.82%), Query Frame = 1

Query: 1   MVDRERDRDRGRDRDKDRDRDRYKD-----RERDRDHRERDRRRDREDRDHRERDR-DRE 60
           M DRER+R+R RDR +DR+R R +D     R+R+RD  +RDR RD+ DRD RERD+ DR+
Sbjct: 1   MTDRERERERDRDRTRDRERKRRRDKDDRDRDRERDKGDRDRERDKGDRD-RERDKGDRD 60

Query: 61  RRRDQDDRDRERERGRSKRSLTPDRSR----SRRSRTRSPDRHRSR-SLSRTPEDRPSRR 120
           R RD+DDRDRERER +  R    +R R    S+RSR+RSPDR RSR + S +P +R  RR
Sbjct: 61  RERDKDDRDREREREKDDRDRERERDRDRVHSKRSRSRSPDRGRSRHARSPSPSERSHRR 120

Query: 121 RH-RSPSPDHARKRHRRDLTADDEKERQKAVSDFVDGIAKEQHKQKNNKDNVGGGEVVEE 180
           RH R+P+PD  RKRHRR+   ++ KE +KAVSDFVDGIAKEQ  Q+  KDN G      E
Sbjct: 121 RHHRTPTPDQPRKRHRRESVEEEHKETKKAVSDFVDGIAKEQ--QQKQKDNGG------E 180

Query: 181 AMADEDEMEMMKKLGIPVGFDSTKGKPVPGADVSGVRVVTKRQPRQYMNRRGGFNRPLPA 233
           A  +EDE+EMMK LGIPVGFDSTKGKPVPGADVSGVR VTKRQPRQYMNRRGGFNRPLPA
Sbjct: 181 AEGNEDEVEMMKMLGIPVGFDSTKGKPVPGADVSGVRAVTKRQPRQYMNRRGGFNRPLPA 235

BLAST of Cucsa.081160.3 vs. TrEMBL
Match: A0A0L9TEY8_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan627s003600 PE=4 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 1.5e-72
Identity = 160/238 (67.23%), Postives = 184/238 (77.31%), Query Frame = 1

Query: 3   DRERDR-DRGRDRDK-DRDRDRYK-DRERDRDHRERDRRRDREDRDHRERD---RDRERR 62
           DRERD+ DR R+RDK DRDR+R K DR+R+RD  +RDR RD+ DRD RERD   RDRER 
Sbjct: 32  DRERDKGDRDRERDKGDRDRERDKGDRDRERDKGDRDRERDKGDRD-RERDKDDRDRERE 91

Query: 63  RDQDDRDRERERGRSKRSLTPDRSRSRRSRTRSPDRHRSR-SLSRTPEDRPSRRRH-RSP 122
           R++DDRDRERER R       DR  S+RSR+RSPDR RSR + S +P +R  RRRH R+P
Sbjct: 92  REKDDRDRERERDR-------DRVHSKRSRSRSPDRGRSRHARSPSPSERSHRRRHHRTP 151

Query: 123 SPDHARKRHRRDLTADDEKERQKAVSDFVDGIAKEQHKQKNNKDNVGGGEVVEEAMADED 182
           +PD  RKRHRR+   ++ KE +KAVSDFVDGIAKEQ  Q+  KDN G      EA  +ED
Sbjct: 152 TPDQPRKRHRRESVEEEHKETKKAVSDFVDGIAKEQ--QQKQKDNGG------EAEGNED 211

Query: 183 EMEMMKKLGIPVGFDSTKGKPVPGADVSGVRVVTKRQPRQYMNRRGGFNRPLPAERSR 233
           E+EMMK LGIPVGFDSTKGKPVPGADVSGVR VTKRQPRQYMNRRGGFNRPLPAER+R
Sbjct: 212 EVEMMKMLGIPVGFDSTKGKPVPGADVSGVRAVTKRQPRQYMNRRGGFNRPLPAERNR 253

BLAST of Cucsa.081160.3 vs. TrEMBL
Match: A0A0L9TEY8_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan627s003600 PE=4 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 9.2e-70
Identity = 154/262 (58.78%), Postives = 183/262 (69.85%), Query Frame = 1

Query: 1   MVDRERDRDRGRDRDKDRDRDRYKD-----RERDRDHRERDRRRDREDRDHRERDR-DRE 60
           M DRER+R+R RDR +DR+R R +D     R+R+RD  +RDR RD+ DRD RERD+ DR+
Sbjct: 1   MTDRERERERDRDRTRDRERKRRRDKDDRDRDRERDKGDRDRERDKGDRD-RERDKGDRD 60

Query: 61  RRRDQDDRDRERERGRSKRSLTP----------------------DRSRSRRSRTRSPDR 120
           R RD+ DRDRER++G   R                          DR  S+RSR+RSPDR
Sbjct: 61  RERDKGDRDRERDKGDRDRERDKDDRDREREREKDDRDRERERDRDRVHSKRSRSRSPDR 120

Query: 121 HRSR-SLSRTPEDRPSRRR-HRSPSPDHARKRHRRDLTADDEKERQKAVSDFVDGIAKEQ 180
            RSR + S +P +R  RRR HR+P+PD  RKRHRR+   ++ KE +KAVSDFVDGIAKEQ
Sbjct: 121 GRSRHARSPSPSERSHRRRHHRTPTPDQPRKRHRRESVEEEHKETKKAVSDFVDGIAKEQ 180

Query: 181 HKQKNNKDNVGGGEVVEEAMADEDEMEMMKKLGIPVGFDSTKGKPVPGADVSGVRVVTKR 233
             Q+  KDN G      EA  +EDE+EMMK LGIPVGFDSTKGKPVPGADVSGVR VTKR
Sbjct: 181 --QQKQKDNGG------EAEGNEDEVEMMKMLGIPVGFDSTKGKPVPGADVSGVRAVTKR 240


HSP 2 Score: 278.5 bits (711), Expect = 7.5e-72
Identity = 153/242 (63.22%), Postives = 178/242 (73.55%), Query Frame = 1

Query: 1   MVDRERDRDRGRDRDKDRDRDRYKDRE-RDRDHRERDRRRDREDRDHRERDRDRERRRDQ 60
           M DRERDRDR RDR +DR+R R +D++ RDR+  + DR RD+ DR+  + DRDRER +D 
Sbjct: 1   MTDRERDRDRDRDRIRDRERKRRRDKDDRDRERDKGDRERDKGDRERDKGDRDRERDKDD 60

Query: 61  DDRDRERERGRSKRSLTP-------DRSRSRRSRTRSPDRHRSR-SLSRTPEDRPSRRRH 120
            DRDRERER R +            DR RS+RSR+RSPDR RSR + S +P +R  RRRH
Sbjct: 61  RDRDREREREREREREREKDERDRDDRVRSKRSRSRSPDRGRSRHARSPSPSERSHRRRH 120

Query: 121 -RSPSPDHARKRHRRDLTADDEKERQKAVSDFVDGIAKEQHKQKNNKDNVGGGEVVEEAM 180
            R+PSPD  RKRHRR+   ++ KE +KAVSDFVDGIA EQ  Q+  KDN G      EA 
Sbjct: 121 HRTPSPDQPRKRHRRESAEEEHKETKKAVSDFVDGIAMEQ--QQKQKDNGG------EAE 180

Query: 181 ADEDEMEMMKKLGIPVGFDSTKGKPVPGADVSGVRVVTKRQPRQYMNRRGGFNRPLPAER 233
            DEDE+EMMK LGIPVGFDSTKGKPV GADVSGVR VTKRQPRQYMNRRGGFNRPLPAER
Sbjct: 181 GDEDELEMMKMLGIPVGFDSTKGKPVAGADVSGVRAVTKRQPRQYMNRRGGFNRPLPAER 234

BLAST of Cucsa.081160.3 vs. TrEMBL
Match: M5XT32_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011504mg PE=4 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 7.3e-67
Identity = 147/212 (69.34%), Postives = 169/212 (79.72%), Query Frame = 1

Query: 33  RERDRRRDREDRDHRERDRDRERRRDQDDRDRERERGR--SKRSLTPDRSRSR--RSRTR 92
           RERDR RDRE   +R+RDRDRERRRD+DDRDR+R+R R  SKRS TP+R+R R  RSRTR
Sbjct: 4   RERDRDRDRER--NRDRDRDRERRRDRDDRDRDRDRDRVRSKRSRTPERTRPRHTRSRTR 63

Query: 93  SPDRHRSR----SLSRTPEDRPSRRRH---RSPSPDHARKRHRRDLTADDEKERQKA-VS 152
           SPDR+RSR    S SR+P+DR  RRRH   RSPS + ARKR RR+ TA+DE+ERQKA V+
Sbjct: 64  SPDRYRSRTRSVSRSRSPDDRSHRRRHHHNRSPSAE-ARKRQRREPTAEDERERQKAAVA 123

Query: 153 DFVDGIAKEQHKQKNNKDNVGGGEVVEEAMADEDEMEMMKKLGIPVGFDSTKGKPVPGAD 212
           DFV+GIAKEQ  Q+    + GG E +     DEDE+EMMKK GIP GFDSTKGKPVPGAD
Sbjct: 124 DFVEGIAKEQKTQQKQSSDGGGDEGL-----DEDEIEMMKKFGIPTGFDSTKGKPVPGAD 183

Query: 213 VSGVRVVTKRQPRQYMNRRGGFNRPLPAERSR 233
           VSG+R VTKRQPRQYMNRRGGFNRPLPAER+R
Sbjct: 184 VSGIRAVTKRQPRQYMNRRGGFNRPLPAERNR 207

BLAST of Cucsa.081160.3 vs. TAIR10
Match: AT5G57370.1 (AT5G57370.1 unknown protein)

HSP 1 Score: 213.0 bits (541), Expect = 2.0e-55
Identity = 127/227 (55.95%), Postives = 158/227 (69.60%), Query Frame = 1

Query: 19  DRDRYKDRERDRDHR-ERDRRRDREDRDHRERDRDRERRRDQDDRDRERERG---RSKRS 78
           +RDR ++RERD+D   +RDRRR R+DRD R+RDRDRER     DR+R+R+RG   +  RS
Sbjct: 3   ERDR-RERERDKDREPDRDRRRGRDDRD-RDRDRDRER-----DRERDRDRGLRNKKSRS 62

Query: 79  LTPDRSRSRRSRTRSPDRHRSRSLSRTPE---DRPSRRRHRSPSPDHARKRHRRDLTADD 138
            TPD   +R    RSP+R+RSRS S   +   DR    R RSPSPD   ++  R  + DD
Sbjct: 63  RTPDH-HARARHVRSPERYRSRSRSIDRDRDRDRQRHHRRRSPSPDAPSRKRPRQGSVDD 122

Query: 139 EKER--QKAVSDFVDG----IAKEQHKQKNNKDNVGGGEVVEEAMADEDEMEMMKKLGIP 198
           EKER  ++ V+D VD     I K++ KQ ++  + GGGE  +E   D +E+EMMK LGIP
Sbjct: 123 EKERNRKRVVTDSVDENASTITKKKDKQPSDAADNGGGE--DEEGMDVNEIEMMKMLGIP 182

Query: 199 VGFDSTKGKPVPGADVSGVRVVTKRQPRQYMNRRGGFNRPLPAERSR 233
            GFDSTKGKPV GADVSG+R VTKRQPRQYMNRRGGFNRPLPAER+R
Sbjct: 183 TGFDSTKGKPVAGADVSGIRAVTKRQPRQYMNRRGGFNRPLPAERNR 219


HSP 2 Score: 47.0 bits (110), Expect = 1.9e-05
Identity = 40/127 (31.50%), Postives = 52/127 (40.94%), Query Frame = 1

Query: 3   DRERDRDRGRDRDKDRDR----------------------------------DRYKDRER 62
           DR+RDRDR RDR++DRDR                                  DR +DR+R
Sbjct: 29  DRDRDRDRERDRERDRDRGLRNKKSRSRTPDHHARARHVRSPERYRSRSRSIDRDRDRDR 88

Query: 63  DRDHRERDRRRDREDRDHRERDRDRERRRDQDDRDRERERGRSKRSLTPDRSRSRRSRTR 96
            R HR   RR    D   R+R R       Q   D E+ER R KR +T     +  + T+
Sbjct: 89  QRHHR---RRSPSPDAPSRKRPR-------QGSVDDEKERNR-KRVVTDSVDENASTITK 144

BLAST of Cucsa.081160.3 vs. TAIR10
Match: AT1G60900.1 (AT1G60900.1 U2 snRNP auxilliary factor, large subunit, splicing factor)

HSP 1 Score: 102.8 bits (255), Expect = 2.9e-22
Identity = 60/123 (48.78%), Postives = 74/123 (60.16%), Query Frame = 1

Query: 3   DRERDRDRGRDRDKDRDRDRYKDRERDRDH-RERDRRRDREDRDHRERDRDRERRRDQDD 62
           +++RDR++ RDR+K RDRDR K R+RDRD  R +DR+RDR  RD R RDR RER   +DD
Sbjct: 63  NKDRDREKDRDREKSRDRDREKSRDRDRDRERSKDRQRDRHHRD-RHRDRSRERSEKRDD 122

Query: 63  RDRERERGRSKRSLTPDRSRSRRSRTRSPDRHRSRSLSRTPEDRPSRRRHRSPSPDHARK 122
            D +  R    RS   DR RSR        R RSRS SR+  +R SR  HR  S   +R 
Sbjct: 123 LDDDHHR----RSRDRDRRRSRDRDREVRHRRRSRSRSRSRSERRSRSEHRHKSEHRSRS 180

Query: 123 RHR 125
           R R
Sbjct: 183 RSR 180


HSP 2 Score: 98.2 bits (243), Expect = 7.1e-21
Identity = 58/135 (42.96%), Postives = 76/135 (56.30%), Query Frame = 1

Query: 3   DRERDRDRGRDRDKDRDRDRYKDRERDRDH-RERDRRRDREDRD-HRERDRDRERRRDQD 62
           +++RDR++ RDR+K RDRDR K R+RDRD  R +DR+RDR  RD HR+R R+R  +RD  
Sbjct: 63  NKDRDREKDRDREKSRDRDREKSRDRDRDRERSKDRQRDRHHRDRHRDRSRERSEKRDDL 122

Query: 63  DRDRERERGRSKRSLTPDRSRS----RRSRTRSPDRHRSRSLSRTPEDRPSRRRHRSPSP 122
           D D  R      R  + DR R     RRSR+RS  R   RS S        R R RS S 
Sbjct: 123 DDDHHRRSRDRDRRRSRDRDREVRHRRRSRSRSRSRSERRSRSEHRHKSEHRSRSRSRSR 182

Query: 123 DHARKRHRRDLTADD 132
             +++R   D+   D
Sbjct: 183 SKSKRRSGFDMAPPD 197


HSP 3 Score: 71.6 bits (174), Expect = 7.1e-13
Identity = 54/121 (44.63%), Postives = 64/121 (52.89%), Query Frame = 1

Query: 13  DRDKDRDRDRYKDRERDRDHRERDRRRDREDRDHRERDRDRERRRDQD---DRDRERERG 72
           D  KD  +   KD E  RD   +D  +DR+    RE+DRDRE+ RD+D    RDR+R+R 
Sbjct: 41  DLKKDSSKISEKDNENGRD---KDGNKDRD----REKDRDREKSRDRDREKSRDRDRDRE 100

Query: 73  RSKRSLTPDRSRSRRSRTRSPDRHRSRSLSRTPED----RPSRRRHRSPSPDHARK-RHR 126
           RSK     DR R R  R R  DR R RS  R   D    R SR R R  S D  R+ RHR
Sbjct: 101 RSK-----DRQRDRHHRDRHRDRSRERSEKRDDLDDDHHRRSRDRDRRRSRDRDREVRHR 149


HSP 4 Score: 39.3 bits (90), Expect = 3.9e-03
Identity = 31/121 (25.62%), Postives = 51/121 (42.15%), Query Frame = 1

Query: 40  DREDRDHRERDRDRERRRDQDDRDRERERGRSKRSLTPDRSRSRRSRTRSPDRHRSRSLS 99
           D + R+  +  +D  +  ++D+     E GR K            ++ R  ++ R R  S
Sbjct: 33  DSKSRESHDLKKDSSKISEKDN-----ENGRDKDG----------NKDRDREKDRDREKS 92

Query: 100 RTPEDRPS--RRRHRSPSPDHARKRHRRDLTADDEKERQKAVSDFVDGIAKEQHKQKNNK 159
           R  +   S  R R R  S D  R RH RD   D  +ER +   D  D    + H++  ++
Sbjct: 93  RDRDREKSRDRDRDRERSKDRQRDRHHRDRHRDRSRERSEKRDDLDD----DHHRRSRDR 134

BLAST of Cucsa.081160.3 vs. TAIR10
Match: AT5G53440.1 (AT5G53440.1 unknown protein)

HSP 1 Score: 91.3 bits (225), Expect = 8.6e-19
Identity = 80/249 (32.13%), Postives = 115/249 (46.18%), Query Frame = 1

Query: 5   ERDRDRGRDRDKDRDRDRYKDRERDRDHRERDRRRDREDRDH-RERDRDRERRRDQD--- 64
           ERDR+R RDRD+D +RDR +DR+RDR+ R+RDRR    DR H R+ DRDR R RD+D   
Sbjct: 317 ERDRERTRDRDRDYERDRDRDRDRDRE-RDRDRRDYEHDRYHDRDWDRDRSRDRDRDHER 376

Query: 65  DRDRERERGRSKRSLTPDRSRSRRSRTRSPDRHRSRSLSRTPEDRPSRRRHRSP------ 124
           DR  +RE+ RS R    D  RS+  R R  DR  SR   ++   +  R   RSP      
Sbjct: 377 DRTHDREKDRS-RDYYHDGKRSKSDRERDNDRDVSRLDDQSGRYKDRRDGRRSPDYQDYQ 436

Query: 125 -----------SPDHARKRHRRDLTADDEKERQKAVSD-FVDGIAKEQHKQKNNKDNVGG 184
                       PD    R  R L++   +E     SD    G +  +  + +     G 
Sbjct: 437 DVITGSRSSRVEPDGDMTRPERQLSSSVVQEENGNASDQITKGASSREVAELSGGSERGT 496

Query: 185 GEVVEEAMADEDEMEMMKKLGIPVGFDSTKGKPVPGADVSGVRVVTKRQPRQYMNRRGGF 232
            + V E  A+ ++  ++ +      F + K  P P  + S      +R+     N RGG 
Sbjct: 497 RQKVSEKTANMED-GVLGEFPAERSF-AAKASPRPMVERSPSSTSLERR----YNNRGGA 556


HSP 2 Score: 74.7 bits (182), Expect = 8.3e-14
Identity = 51/137 (37.23%), Postives = 68/137 (49.64%), Query Frame = 1

Query: 6   RDRDRGRDRDKDRDRDRYKDRERDRDHR-----ERDRRRDREDRD-HRERDRDRERRRDQ 65
           RD  + + + +D D     D E D  H      + DR  DR +RD  R RDRDR+  RD+
Sbjct: 275 RDESKKKSKFQDNDHGHEPDSELDGYHERERNRDYDRESDRNERDRERTRDRDRDYERDR 334

Query: 66  D-DRDRERERGRSKRSLTPDRSRSRR-SRTRSPDRHRSRSLSRTPEDRPSRRRHRSPSPD 125
           D DRDR+RER R +R    DR   R   R RS DR R     RT +    R + RS    
Sbjct: 335 DRDRDRDRERDRDRRDYEHDRYHDRDWDRDRSRDRDRDHERDRTHD----REKDRSRDYY 394

Query: 126 HARKRHRRDLTADDEKE 135
           H  KR + D   D++++
Sbjct: 395 HDGKRSKSDRERDNDRD 407

BLAST of Cucsa.081160.3 vs. TAIR10
Match: AT1G20920.1 (AT1G20920.1 P-loop containing nucleoside triphosphate hydrolases superfamily protein)

HSP 1 Score: 87.8 bits (216), Expect = 9.5e-18
Identity = 64/183 (34.97%), Postives = 99/183 (54.10%), Query Frame = 1

Query: 4   RERDRDRGRDRDKDRDRDRYKDRERDRDHRERDRRRDREDRDHRERDRDRERRR----DQ 63
           RE +RDRG+DR +DR+R+  KD+ER+R+     R R+RE+R+ +ER ++RERR     ++
Sbjct: 124 REHERDRGKDRKRDREREERKDKEREREKDRERREREREERE-KERVKERERREREDGER 183

Query: 64  DDRDRERERGRSKRSLTPDRSRSRRSRTRSPDRHRSRSLSRTPEDRPSRRRHRSPSPDHA 123
           D R+RE+ERG            SRR+R    +R RSR +     D   +R          
Sbjct: 184 DRREREKERG------------SRRNR----ERERSREVGNEESDDDVKR---------D 243

Query: 124 RKRHRRDLTADDEKERQKAVSDFVDGIAKEQHKQKNNKDNVGGGEVVEEAMADEDEMEMM 183
            KR R++     EKER+K+V        ++  K+K+ +DN   GE  E+   +E+  +  
Sbjct: 244 LKRRRKEGGERKEKEREKSVGR--SSRHEDSPKRKSVEDN---GEKKEKKTREEELEDEQ 275


HSP 2 Score: 84.7 bits (208), Expect = 8.1e-17
Identity = 68/189 (35.98%), Postives = 103/189 (54.50%), Query Frame = 1

Query: 2   VDRERDRDRGRDRDKDR-----DRDRYKDRERDRDHRER-DRRRDRE-DRDHRERDRD-R 61
           V+ E +RD+ R  +K+R     +RDR KDR+RDR+  ER D+ R+RE DR+ RER+R+ R
Sbjct: 105 VEEEDERDKRRVNEKERGHREHERDRGKDRKRDREREERKDKEREREKDRERREREREER 164

Query: 62  ERRRDQDDRDRERERGRSKRSLTPDRSRSRRSRTRSPDRHRSRSLSRTPEDRPSRRRHRS 121
           E+ R ++   RERE G   R    +R + R SR R+ +R RSR +     D   +R    
Sbjct: 165 EKERVKERERREREDGERDRR---EREKERGSR-RNRERERSREVGNEESDDDVKR---- 224

Query: 122 PSPDHARKRHRRDLTADDEKERQKAVSDFVDGIAKEQHKQKNNKDNVGGGEVVEEAMADE 181
                  KR R++     EKER+K+V        ++  K+K+ +DN   GE  E+   +E
Sbjct: 225 -----DLKRRRKEGGERKEKEREKSVGR--SSRHEDSPKRKSVEDN---GEKKEKKTREE 275

Query: 182 DEMEMMKKL 183
           +  +  KKL
Sbjct: 285 ELEDEQKKL 275


HSP 3 Score: 70.1 bits (170), Expect = 2.1e-12
Identity = 54/199 (27.14%), Postives = 90/199 (45.23%), Query Frame = 1

Query: 4   RERDRDRGRDRDKDRDRDRYKDRERDR-------DHRERDRRRDREDRDHRERDRDRERR 63
           R+RDR   R +DK  ++ R KDR + R       D  +RD   +RE R  +ER+R   RR
Sbjct: 27  RDRDRSNERKKDKGSEKRREKDRRKKRVKSSDSEDDYDRDDDEEREKRKEKERER---RR 86

Query: 64  RDQDDRDRERERGRS-----------KRSLTPDRSRSRRSRTRSPDRHRSRSLSRTPEDR 123
           RD+D   R  ER +S           +R       + R  R    DR + R   R  E+R
Sbjct: 87  RDKDRVKRRSERRKSSDSEDDVEEEDERDKRRVNEKERGHREHERDRGKDRKRDREREER 146

Query: 124 PSRRRHRSPSPDHARKRHRRDLTADDEKERQKAVSDFVDGIAKEQHKQ---KNNKDNVGG 182
             + R R    +  R+R R +   +  KER++   +  +   +E+ K+   + N++    
Sbjct: 147 KDKEREREKDRER-REREREEREKERVKERERREREDGERDRREREKERGSRRNRERERS 206

BLAST of Cucsa.081160.3 vs. TAIR10
Match: AT2G40650.1 (AT2G40650.1 PRP38 family protein)

HSP 1 Score: 87.4 bits (215), Expect = 1.2e-17
Identity = 52/111 (46.85%), Postives = 69/111 (62.16%), Query Frame = 1

Query: 3   DRERDRDRGRDRDKDRDRDRYKDRERDRDHRERDRRRDREDRDHRERDRDRERRRDQ-DD 62
           +RERDRDR RD  + RDRD  +D + DRDH +RD  R+R       RDRDRER RD   +
Sbjct: 218 ERERDRDRRRDSHRHRDRDYDRDYDMDRDH-DRDYERER----GHGRDRDRERDRDHYRE 277

Query: 63  RDRERERGRSKRSLTPDRSRSRRSRTRSPDRHRSRSLSRTPEDRPSRRRHR 113
           RDR+RERGR +     DR+R RRSR+RS DR R  +      + P +++ +
Sbjct: 278 RDRDRERGRDRERDRRDRAR-RRSRSRSRDRKRHETDDVRDREEPKKKKEK 322


HSP 2 Score: 80.5 bits (197), Expect = 1.5e-15
Identity = 57/138 (41.30%), Postives = 71/138 (51.45%), Query Frame = 1

Query: 3   DRERDRDRG-RDRDKDRDRDRYKDRERDRDHRERDRRRDREDRDHRERDRDRERRRDQD- 62
           + E++ + G  D  +D    R K  ER+R   ERDR R R+   HR+RD DR+   D+D 
Sbjct: 191 EEEKEENEGIADGSEDEMDQRRKSPERER---ERDRDRRRDSHRHRDRDYDRDYDMDRDH 250

Query: 63  DRDRERERGRSKRSLTPDRSRSR-RSRTRSPDRHRSRSLSRTPEDRPSRRRHRSPSPDHA 122
           DRD ERERG  +     DR R R R   R  DR R R   R   DR  R R RS S    
Sbjct: 251 DRDYERERGHGR-----DRDRERDRDHYRERDRDRERGRDR-ERDRRDRARRRSRSRSRD 310

Query: 123 RKRHRRDLTADDEKERQK 138
           RKRH  D   D E+ ++K
Sbjct: 311 RKRHETDDVRDREEPKKK 319


HSP 3 Score: 78.6 bits (192), Expect = 5.8e-15
Identity = 59/138 (42.75%), Postives = 71/138 (51.45%), Query Frame = 1

Query: 3   DRERDRDRGRD----RDKDRDRDRYKDRERDRDH-RERDRRRDRE---DRDH-RERDRDR 62
           +RERDRDR RD    RD+D DRD   DR+ DRD+ RER   RDR+   DRDH RERDRDR
Sbjct: 218 ERERDRDRRRDSHRHRDRDYDRDYDMDRDHDRDYERERGHGRDRDRERDRDHYRERDRDR 277

Query: 63  ER----RRDQDDRDRERERGRSKRSLTPDRSRSRRS--RTRSPDRHRSRSLSRTPEDRPS 122
           ER     RD+ DR R R R RS+     DR R      R R   + +     +  ED   
Sbjct: 278 ERGRDRERDRRDRARRRSRSRSR-----DRKRHETDDVRDREEPKKKKEKKEKMKEDGTD 337

Query: 123 RRRHRSPSPDHARKRHRR 126
                 P+P+ A     R
Sbjct: 338 H-----PNPEIAEMNRLR 345

BLAST of Cucsa.081160.3 vs. NCBI nr
Match: gi|449457171|ref|XP_004146322.1| (PREDICTED: U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein [Cucumis sativus])

HSP 1 Score: 475.3 bits (1222), Expect = 6.0e-131
Identity = 232/232 (100.00%), Postives = 232/232 (100.00%), Query Frame = 1

Query: 1   MVDRERDRDRGRDRDKDRDRDRYKDRERDRDHRERDRRRDREDRDHRERDRDRERRRDQD 60
           MVDRERDRDRGRDRDKDRDRDRYKDRERDRDHRERDRRRDREDRDHRERDRDRERRRDQD
Sbjct: 1   MVDRERDRDRGRDRDKDRDRDRYKDRERDRDHRERDRRRDREDRDHRERDRDRERRRDQD 60

Query: 61  DRDRERERGRSKRSLTPDRSRSRRSRTRSPDRHRSRSLSRTPEDRPSRRRHRSPSPDHAR 120
           DRDRERERGRSKRSLTPDRSRSRRSRTRSPDRHRSRSLSRTPEDRPSRRRHRSPSPDHAR
Sbjct: 61  DRDRERERGRSKRSLTPDRSRSRRSRTRSPDRHRSRSLSRTPEDRPSRRRHRSPSPDHAR 120

Query: 121 KRHRRDLTADDEKERQKAVSDFVDGIAKEQHKQKNNKDNVGGGEVVEEAMADEDEMEMMK 180
           KRHRRDLTADDEKERQKAVSDFVDGIAKEQHKQKNNKDNVGGGEVVEEAMADEDEMEMMK
Sbjct: 121 KRHRRDLTADDEKERQKAVSDFVDGIAKEQHKQKNNKDNVGGGEVVEEAMADEDEMEMMK 180

Query: 181 KLGIPVGFDSTKGKPVPGADVSGVRVVTKRQPRQYMNRRGGFNRPLPAERSR 233
           KLGIPVGFDSTKGKPVPGADVSGVRVVTKRQPRQYMNRRGGFNRPLPAERSR
Sbjct: 181 KLGIPVGFDSTKGKPVPGADVSGVRVVTKRQPRQYMNRRGGFNRPLPAERSR 232

BLAST of Cucsa.081160.3 vs. NCBI nr
Match: gi|659107269|ref|XP_008453595.1| (PREDICTED: U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein [Cucumis melo])

HSP 1 Score: 460.3 bits (1183), Expect = 2.0e-126
Identity = 225/232 (96.98%), Postives = 227/232 (97.84%), Query Frame = 1

Query: 1   MVDRERDRDRGRDRDKDRDRDRYKDRERDRDHRERDRRRDREDRDHRERDRDRERRRDQD 60
           MVDRERDRDRGRDRDKDRDRDRYKDRERDRDHRERDRRRDREDRDHRERDRDRERRRD D
Sbjct: 1   MVDRERDRDRGRDRDKDRDRDRYKDRERDRDHRERDRRRDREDRDHRERDRDRERRRDLD 60

Query: 61  DRDRERERGRSKRSLTPDRSRSRRSRTRSPDRHRSRSLSRTPEDRPSRRRHRSPSPDHAR 120
           DRDRERERGRSKRS TPDRSRSRRSRTRSPDRHRSRSLSRTPEDR SRRRHRSPSPDHAR
Sbjct: 61  DRDRERERGRSKRSHTPDRSRSRRSRTRSPDRHRSRSLSRTPEDRSSRRRHRSPSPDHAR 120

Query: 121 KRHRRDLTADDEKERQKAVSDFVDGIAKEQHKQKNNKDNVGGGEVVEEAMADEDEMEMMK 180
           KRHRRDLTADDEKERQKAVSDFVDGIAKEQHKQKNNKDN G GEVVEEAMADEDE+EMMK
Sbjct: 121 KRHRRDLTADDEKERQKAVSDFVDGIAKEQHKQKNNKDNAGSGEVVEEAMADEDEIEMMK 180

Query: 181 KLGIPVGFDSTKGKPVPGADVSGVRVVTKRQPRQYMNRRGGFNRPLPAERSR 233
           KLGIPVGFDSTKGKPVPGADVSGVRVVTKRQPRQYMNRRGGFNRPLPAER+R
Sbjct: 181 KLGIPVGFDSTKGKPVPGADVSGVRVVTKRQPRQYMNRRGGFNRPLPAERTR 232

BLAST of Cucsa.081160.3 vs. NCBI nr
Match: gi|950996098|ref|XP_014505611.1| (PREDICTED: U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein [Vigna radiata var. radiata])

HSP 1 Score: 285.4 bits (729), Expect = 8.8e-74
Identity = 162/253 (64.03%), Postives = 187/253 (73.91%), Query Frame = 1

Query: 1   MVDRERDRDRGRDRDK---------DRDRDRYKDRERDRDHRERD---RRRDREDRDHRE 60
           M DRER+RDR R RD+         DRDRDR +DRERD+  RERD   R RD+ DRD RE
Sbjct: 1   MTDRERERDRERTRDRERKRRRDKDDRDRDRDRDRERDKGERERDKGDRERDKGDRD-RE 60

Query: 61  RDR-DRERRRDQDDRDRERERGRSK------RSLTPDRSRSRRSRTRSPDRHRSRSL-SR 120
           RD+ DR+R RD+DDRDR+RER R K      R    DR RS+RSR+RSPDR RSR + S 
Sbjct: 61  RDKGDRDRERDKDDRDRDREREREKDDRDRERERDRDRVRSKRSRSRSPDRGRSRHVRSP 120

Query: 121 TPEDRPSRRRH-RSPSPDHARKRHRRDLTADDEKERQKAVSDFVDGIAKEQHKQKNNKDN 180
           +P +R  RRRH R+P+PD  RKRHRR+   ++ KE +KAVSDFVDGIAKEQ  Q+  K+N
Sbjct: 121 SPSERSHRRRHHRTPTPDQPRKRHRRESVEEEHKETKKAVSDFVDGIAKEQ--QQKQKEN 180

Query: 181 VGGGEVVEEAMADEDEMEMMKKLGIPVGFDSTKGKPVPGADVSGVRVVTKRQPRQYMNRR 233
            G      EA  +EDE+EMMK LGIPVGFDSTKGKPVPGADVSGVR VTKRQPRQYMNRR
Sbjct: 181 GG------EAEGNEDEVEMMKMLGIPVGFDSTKGKPVPGADVSGVRAVTKRQPRQYMNRR 240

BLAST of Cucsa.081160.3 vs. NCBI nr
Match: gi|965660328|dbj|BAT76747.1| (hypothetical protein VIGAN_01479700 [Vigna angularis var. angularis])

HSP 1 Score: 283.1 bits (723), Expect = 4.4e-73
Identity = 156/244 (63.93%), Postives = 185/244 (75.82%), Query Frame = 1

Query: 1   MVDRERDRDRGRDRDKDRDRDRYKD-----RERDRDHRERDRRRDREDRDHRERDR-DRE 60
           M DRER+R+R RDR +DR+R R +D     R+R+RD  +RDR RD+ DRD RERD+ DR+
Sbjct: 1   MTDRERERERDRDRTRDRERKRRRDKDDRDRDRERDKGDRDRERDKGDRD-RERDKGDRD 60

Query: 61  RRRDQDDRDRERERGRSKRSLTPDRSR----SRRSRTRSPDRHRSR-SLSRTPEDRPSRR 120
           R RD+DDRDRERER +  R    +R R    S+RSR+RSPDR RSR + S +P +R  RR
Sbjct: 61  RERDKDDRDREREREKDDRDRERERDRDRVHSKRSRSRSPDRGRSRHARSPSPSERSHRR 120

Query: 121 RH-RSPSPDHARKRHRRDLTADDEKERQKAVSDFVDGIAKEQHKQKNNKDNVGGGEVVEE 180
           RH R+P+PD  RKRHRR+   ++ KE +KAVSDFVDGIAKEQ  Q+  KDN G      E
Sbjct: 121 RHHRTPTPDQPRKRHRRESVEEEHKETKKAVSDFVDGIAKEQ--QQKQKDNGG------E 180

Query: 181 AMADEDEMEMMKKLGIPVGFDSTKGKPVPGADVSGVRVVTKRQPRQYMNRRGGFNRPLPA 233
           A  +EDE+EMMK LGIPVGFDSTKGKPVPGADVSGVR VTKRQPRQYMNRRGGFNRPLPA
Sbjct: 181 AEGNEDEVEMMKMLGIPVGFDSTKGKPVPGADVSGVRAVTKRQPRQYMNRRGGFNRPLPA 235

BLAST of Cucsa.081160.3 vs. NCBI nr
Match: gi|920682215|gb|KOM28987.1| (hypothetical protein LR48_Vigan627s003600 [Vigna angularis])

HSP 1 Score: 280.8 bits (717), Expect = 2.2e-72
Identity = 160/238 (67.23%), Postives = 184/238 (77.31%), Query Frame = 1

Query: 3   DRERDR-DRGRDRDK-DRDRDRYK-DRERDRDHRERDRRRDREDRDHRERD---RDRERR 62
           DRERD+ DR R+RDK DRDR+R K DR+R+RD  +RDR RD+ DRD RERD   RDRER 
Sbjct: 32  DRERDKGDRDRERDKGDRDRERDKGDRDRERDKGDRDRERDKGDRD-RERDKDDRDRERE 91

Query: 63  RDQDDRDRERERGRSKRSLTPDRSRSRRSRTRSPDRHRSR-SLSRTPEDRPSRRRH-RSP 122
           R++DDRDRERER R       DR  S+RSR+RSPDR RSR + S +P +R  RRRH R+P
Sbjct: 92  REKDDRDRERERDR-------DRVHSKRSRSRSPDRGRSRHARSPSPSERSHRRRHHRTP 151

Query: 123 SPDHARKRHRRDLTADDEKERQKAVSDFVDGIAKEQHKQKNNKDNVGGGEVVEEAMADED 182
           +PD  RKRHRR+   ++ KE +KAVSDFVDGIAKEQ  Q+  KDN G      EA  +ED
Sbjct: 152 TPDQPRKRHRRESVEEEHKETKKAVSDFVDGIAKEQ--QQKQKDNGG------EAEGNED 211

Query: 183 EMEMMKKLGIPVGFDSTKGKPVPGADVSGVRVVTKRQPRQYMNRRGGFNRPLPAERSR 233
           E+EMMK LGIPVGFDSTKGKPVPGADVSGVR VTKRQPRQYMNRRGGFNRPLPAER+R
Sbjct: 212 EVEMMKMLGIPVGFDSTKGKPVPGADVSGVRAVTKRQPRQYMNRRGGFNRPLPAERNR 253

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SNR27_MOUSE6.4e-2441.54U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein OS=Mus musculus GN=Snrnp... [more]
FRAY2_DICDI4.2e-2347.55Serine/threonine-protein kinase fray2 OS=Dictyostelium discoideum GN=fray2 PE=3 ... [more]
SNR27_HUMAN4.2e-2340.51U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein OS=Homo sapiens GN=SNRNP... [more]
SNR27_XENLA8.7e-2138.58U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein OS=Xenopus laevis GN=snr... [more]
RBM25_HUMAN1.1e-2038.04RNA-binding protein 25 OS=Homo sapiens GN=RBM25 PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A0A0LU61_CUCSA4.2e-131100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G423230 PE=4 SV=1[more]
A0A0S3R800_PHAAN3.1e-7363.93Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G479700 PE=... [more]
A0A0L9TEY8_PHAAN1.5e-7267.23Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan627s003600 PE=4 SV=1[more]
A0A0L9TEY8_PHAAN9.2e-7058.78Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan627s003600 PE=4 SV=1[more]
M5XT32_PRUPE7.3e-6769.34Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011504mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G57370.12.0e-5555.95 unknown protein[more]
AT1G60900.12.9e-2248.78 U2 snRNP auxilliary factor, large subunit, splicing factor[more]
AT5G53440.18.6e-1932.13 unknown protein[more]
AT1G20920.19.5e-1834.97 P-loop containing nucleoside triphosphate hydrolases superfamily pro... [more]
AT2G40650.11.2e-1746.85 PRP38 family protein[more]
Match NameE-valueIdentityDescription
gi|449457171|ref|XP_004146322.1|6.0e-131100.00PREDICTED: U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein [Cucumis sati... [more]
gi|659107269|ref|XP_008453595.1|2.0e-12696.98PREDICTED: U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein [Cucumis melo... [more]
gi|950996098|ref|XP_014505611.1|8.8e-7464.03PREDICTED: U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein [Vigna radiat... [more]
gi|965660328|dbj|BAT76747.1|4.4e-7363.93hypothetical protein VIGAN_01479700 [Vigna angularis var. angularis][more]
gi|920682215|gb|KOM28987.1|2.2e-7267.23hypothetical protein LR48_Vigan627s003600 [Vigna angularis][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR013957SNRNP27
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0044424 intracellular part
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsa.081160Cucsa.081160gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsa.081160.3Cucsa.081160.3-proteinpolypeptide


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.081160.3.three_prime_UTR.2Cucsa.081160.3.three_prime_UTR.2three_prime_UTR
Cucsa.081160.3.three_prime_UTR.1Cucsa.081160.3.three_prime_UTR.1three_prime_UTR


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.081160.3.five_prime_UTR.1Cucsa.081160.3.five_prime_UTR.1five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.081160.3.CDS.1Cucsa.081160.3.CDS.1CDS


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013957Domain of unknown function DUF1777PFAMPF08648DUF1777coord: 54..226
score: 6.4
NoneNo IPR availablePANTHERPTHR31077FAMILY NOT NAMEDcoord: 137..232
score: 9.6E-54coord: 1..111
score: 9.6
NoneNo IPR availablePANTHERPTHR31077:SF1U4/U6.U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 27 KDA PROTEINcoord: 137..232
score: 9.6E-54coord: 1..111
score: 9.6