Cucsa.053520.3 (mRNA) Cucumber (Gy14) v1

BLAST of Cucsa.053520.3 vs. Swiss-Prot
Match: HSP7Q_ARATH (Heat shock 70 kDa protein 16 OS=Arabidopsis thaliana GN=HSP70-16 PE=2 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 3.9e-162
Identity = 274/392 (69.90%), Postives = 338/392 (86.22%), Query Frame = 1

Query: 1   MSVVGFDIGNENCVIAVSRQRGIDVLLNEESQRETPAVIYFGEKQRFLGSAGAASATMNP 60
           MSVVGFD+GNENCVIAV++QRGIDVLLN+ES RE PA++ FGEKQRF+G+A AASATM+P
Sbjct: 1   MSVVGFDVGNENCVIAVAKQRGIDVLLNDESNRENPAMVSFGEKQRFMGAAAAASATMHP 60

Query: 61  RSTISQVKRLIGRNFSEPDVQIELKMFPFKTSEALDGSILVHVKYLGETHTFTPVQIMGM 120
           +STISQ+KRLIGR F EPDVQ +L++FPF+TSE  DG I + ++Y+GE  +F+PVQI+GM
Sbjct: 61  KSTISQLKRLIGRKFREPDVQNDLRLFPFETSEDSDGGIQIRLRYMGEIQSFSPVQILGM 120

Query: 121 LLAHLRDVAEKNLGAPFSDCVIGIPSYFTDLQRRLYWSAAVIAGLKPLRLMHDCTATALS 180
           LL+HL+ +AEK+L  P SDCVIGIPSYFT+ QR  Y  AA IAGL+PLRLMHD TATAL 
Sbjct: 121 LLSHLKQIAEKSLKTPVSDCVIGIPSYFTNSQRLAYLDAAAIAGLRPLRLMHDSTATALG 180

Query: 181 YGIYKTDF-SNTGPIYVAFVDIGHCDTQVSIVSFEPGHMRIMSQTYDRDLGGRDFDEVLF 240
           YGIYKTD  +N+ P Y+ F+DIGHCDTQV + SFE G MR+ S  +DR+LGGRDFDEVLF
Sbjct: 181 YGIYKTDLVANSSPTYIVFIDIGHCDTQVCVASFESGSMRVRSHAFDRNLGGRDFDEVLF 240

Query: 241 SHFVAEFKKNYDIDVNSNVKASIRLRAACEKLKKVLSANLEAVLNIECLMDEKDVKGFIK 300
           +HF  EFK+ Y+IDV +N KA +RLRA+CEK+KKVLSAN EA LNIECLM+EKDV+ FIK
Sbjct: 241 NHFALEFKEKYNIDVYTNTKACVRLRASCEKVKKVLSANAEAQLNIECLMEEKDVRSFIK 300

Query: 301 REEFEKLASGLLEKISIPCTRGLADAGLAVENIHSVELVGSGSRIPAISRLLTSVFKKEP 360
           REEFE+L++GLLE++ +PC + LAD+GL+++ IHSVELVGSGSRIPAIS++L+S+FK+E 
Sbjct: 301 REEFEQLSAGLLERLIVPCQKALADSGLSLDQIHSVELVGSGSRIPAISKMLSSLFKREL 360

Query: 361 SRKLNASECVARGCALQCAMLSPVFRVREYEI 392
            R +NASECVARGCALQCAMLSPVFRVR+YE+
Sbjct: 361 GRTVNASECVARGCALQCAMLSPVFRVRDYEV 392

HSP 2 Score: 249.2 bits (635), Expect = 1.3e-64
Identity = 137/304 (45.07%), Postives = 198/304 (65.13%), Query Frame = 1

Query: 385 RVREYEIGPFQGSNNSNSRVKVRVQLNMNGIITVESATLVE---DTIDQQMPRRDATYSN 444
           ++  + IGPF  S+   +RVKVRVQLN++GI+T++SATL+E   + I  +    +  + +
Sbjct: 457 QISSFMIGPFHISHGEAARVKVRVQLNLHGIVTIDSATLIEYHKENITSEEMISEENHQS 516

Query: 445 TEKMETEFVDSSHSESDARKARGTRRIDIPVSEHIYGGMTKAELLEAQGRELQLAQQDKN 504
           +   +     SS S  +  KA   +R++IPV  ++ G +TK EL EA+ RE  L +QD  
Sbjct: 517 SAMKDGSLDPSSGSIGNEPKA--IKRMEIPVVANVSGALTKDELSEAKQRENSLVEQDLK 576

Query: 505 MEQAKNKKNALESYVYEMRNKLFNTYRSFASDQEREGISSSLQQTEEWLYEDGDDETESA 564
           ME  K+KKNALES+VYEMR+K+ NTYR+ A++ ERE I+ +LQ+TEEWLYEDGDDE+E+A
Sbjct: 577 MESTKDKKNALESFVYEMRDKMLNTYRNTATESERECIARNLQETEEWLYEDGDDESENA 636

Query: 565 YSSKLDGLKKLVDPIINRYEDEEARAQAKAHLLKRISDYRNSGDSLSPQVRALIFEECDK 624
           Y  KL+ +KKL+DPI NR++D E R QA   LLK I+D R + +SL P  +  + +EC K
Sbjct: 637 YIEKLNDVKKLIDPIENRFKDGEERVQASKDLLKTIADNRMAAESLPPPRKNAVLDECHK 696

Query: 625 VEQWLTEKNQQQELLAKNTDPLLWSSEIRTQEEDFDKTCQRILGPVSSHTNSGDSKETNH 684
            E+WL EK  +QE L K+ +P L S+EIR + +  + TC+ I       +NS  +K    
Sbjct: 697 AERWLHEKTTEQESLPKDANPELQSAEIRRKADALNATCKYI-----GKSNSPPAKP--E 751

Query: 685 HNSS 686
           HN S
Sbjct: 757 HNGS 751

BLAST of Cucsa.053520.3 vs. Swiss-Prot
Match: HSP7O_ARATH (Heat shock 70 kDa protein 14 OS=Arabidopsis thaliana GN=HSP70-14 PE=2 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 2.0e-145
Identity = 268/519 (51.64%), Postives = 360/519 (69.36%), Query Frame = 1

Query: 1   MSVVGFDIGNENCVIAVSRQRGIDVLLNEESQRETPAVIYFGEKQRFLGSAGAASATMNP 60
           MSVVGFD GNENC++AV+RQRGIDV+LN+ES RETPA++ FG+KQRF+G+AGAAS  MNP
Sbjct: 1   MSVVGFDFGNENCLVAVARQRGIDVVLNDESNRETPAIVCFGDKQRFIGTAGAASTMMNP 60

Query: 61  RSTISQVKRLIGRNFSEPDVQIELKMFPFKTSEALDGSILVHVKYLGETHTFTPVQIMGM 120
           +++ISQ+KRLIGR FS+P++Q ++K  PF  +E  DG  L+H  YLGE   FTP Q+MGM
Sbjct: 61  KNSISQIKRLIGRQFSDPELQRDIKSLPFSVTEGPDGYPLIHANYLGEKRAFTPTQVMGM 120

Query: 121 LLAHLRDVAEKNLGAPFSDCVIGIPSYFTDLQRRLYWSAAVIAGLKPLRLMHDCTATALS 180
           +L++L+ +AEKNL     DC IGIP YFTDLQRR    AA IAGL PLRL+H+ TATAL+
Sbjct: 121 MLSNLKGIAEKNLNTAVVDCCIGIPVYFTDLQRRAVLDAATIAGLHPLRLIHETTATALA 180

Query: 181 YGIYKTDFSNTGPIYVAFVDIGHCDTQVSIVSFEPGHMRIMSQTYDRDLGGRDFDEVLFS 240
           YGIYKTD   +  + VAF+DIGH   QV I  F+ G ++I+S  +DR LGGRDFDEVLF+
Sbjct: 181 YGIYKTDLPESDQLNVAFIDIGHASMQVCIAGFKKGQLKILSHAFDRSLGGRDFDEVLFN 240

Query: 241 HFVAEFKKNYDIDVNSNVKASIRLRAACEKLKKVLSANLEAVLNIECLMDEKDVKGFIKR 300
           HF A+FK  Y IDV+ N KAS+RLRA CEKLKKVLSAN  A LNIECLMDEKDV+G IKR
Sbjct: 241 HFAAKFKDEYKIDVSQNAKASLRLRATCEKLKKVLSANPLAPLNIECLMDEKDVRGVIKR 300

Query: 301 EEFEKLASGLLEKISIPCTRGLADAGLAVENIHSVELVGSGSRIPAISRLLTSVFKKEPS 360
           EEFE+++  +LE++  P  + L+DAGL VE++H VE++GSGSR+PA+ ++LT  F KEP 
Sbjct: 301 EEFEEISIPILERVKRPLEKALSDAGLTVEDVHMVEVIGSGSRVPAMIKILTEFFGKEPR 360

Query: 361 RKLNASECVARGCALQCAMLSPVFRVREYEIG---PFQ---------------GSNNSNS 420
           R +NASECV+RGCALQCA+LSP F+VRE+++    PF                G+ N  S
Sbjct: 361 RTMNASECVSRGCALQCAILSPTFKVREFQVHESFPFSISLAWKGAASEAQNGGAENQQS 420

Query: 421 RVKVRVQLNMNGIITVESATLVEDTIDQQMPRRDATYSNTEKMETEFVDSSHSESDARKA 480
            +   V    N I +V++ T             D  YS+   ++     S+++    + +
Sbjct: 421 TI---VFPKGNPIPSVKALTFYRSGTFSV----DVQYSDVNDLQAPPKISTYTIGPFQSS 480

Query: 481 RGTR-RIDIPVSEHIYG--GMTKAELLEAQGRELQLAQQ 499
           +G R ++ + V  +++G   +  A LLE +  E+ + ++
Sbjct: 481 KGERAKLKVKVRLNLHGIVSVESATLLEEEEVEVPVTKE 512

HSP 2 Score: 166.0 bits (419), Expect = 1.4e-39
Identity = 87/228 (38.16%), Postives = 140/228 (61.40%), Query Frame = 1

Query: 444 KMETEFVDSSHSESDARKARGTRRIDIPVSEHIYGGMTKAELLEAQGRELQLAQQDKNME 503
           +MET+    S +E+  +K + T   ++P+SE +YG +   E+ +A  +E ++A QD+ ME
Sbjct: 565 QMETD----SKAEAPKKKVKKT---NVPLSELVYGALKTVEVEKAVEKEFEMALQDRVME 624

Query: 504 QAKNKKNALESYVYEMRNKLFNTYRSFASDQEREGISSSLQQTEEWLYEDGDDETESAYS 563
           + K++KNA+ESYVY+MRNKL + Y+ + +D ERE   ++LQ+ E+WLYEDG+DET+  Y 
Sbjct: 625 ETKDRKNAVESYVYDMRNKLSDKYQEYITDSEREAFLANLQEVEDWLYEDGEDETKGVYV 684

Query: 564 SKLDGLKKLVDPIINRYEDEEARAQAKAHLLKRISDYRNSGDSLSPQV-------RALIF 623
           +KL+ LKK+ DP+  RY++   R      L   I+ YR +  S  P+        +  + 
Sbjct: 685 AKLEELKKVGDPVEVRYKESLERGSVIDQLGYCINSYREAAMSTDPKFDHIELAEKQKVL 744

Query: 624 EECDKVEQWLTEKNQQQELLAKNTDPLLWSSEIRTQEEDFDKTCQRIL 665
            EC + E WL  K QQQ+ L K   P L S++++++ E  DK C+ I+
Sbjct: 745 NECVEAEAWLRGKQQQQDTLPKYATPALLSADVKSKAEALDKFCRPIM 785

HSP 3 Score: 58.5 bits (140), Expect = 3.3e-07
Identity = 60/234 (25.64%), Postives = 107/234 (45.73%), Query Frame = 1

Query: 385 RVREYEIGPFQGSNNSNSRVKVRVQLNMNGIITVESATLVEDTIDQQMPRRDATYSNTEK 444
           ++  Y IGPFQ S    +++KV+V+LN++GI++VESATL+E+  + ++P    T  ++E 
Sbjct: 461 KISTYTIGPFQSSKGERAKLKVKVRLNLHGIVSVESATLLEEE-EVEVP---VTKEHSE- 520

Query: 445 METEFVDSSHSESDARKARGTRRIDIPVSEHIYGGM-TKAELLEAQGRELQLAQQDKNME 504
            ET  +DS  + ++A  A G   +++  ++       T   + E+  + +Q+    K   
Sbjct: 521 -ETTKMDSDKASAEAAPASGDCDVNMQDAKDTSDATGTDNGVPESAEKPVQMETDSKAEA 580

Query: 505 QAKNKKNA---LESYVY------EMRNKLFNTYRSFASDQEREGISSSLQQTEEWLYE-- 564
             K  K     L   VY      E+   +   +     D+  E         E ++Y+  
Sbjct: 581 PKKKVKKTNVPLSELVYGALKTVEVEKAVEKEFEMALQDRVMEETKDRKNAVESYVYDMR 640

Query: 565 ----DGDDE--TESAYSSKLDGLKKLVDPIINRYEDEEARAQ-AKAHLLKRISD 600
               D   E  T+S   + L  L+++ D +    EDE      AK   LK++ D
Sbjct: 641 NKLSDKYQEYITDSEREAFLANLQEVEDWLYEDGEDETKGVYVAKLEELKKVGD 688

BLAST of Cucsa.053520.3 vs. Swiss-Prot
Match: HSP7P_ARATH (Heat shock 70 kDa protein 15 OS=Arabidopsis thaliana GN=HSP70-15 PE=1 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 9.7e-145
Identity = 273/530 (51.51%), Postives = 365/530 (68.87%), Query Frame = 1

Query: 1   MSVVGFDIGNENCVIAVSRQRGIDVLLNEESQRETPAVIYFGEKQRFLGSAGAASATMNP 60
           MSVVGFD GNENC++AV+RQRGIDV+LN+ES RETPA++ FG+KQRF+G+AGAAS  MNP
Sbjct: 1   MSVVGFDFGNENCLVAVARQRGIDVVLNDESNRETPAIVCFGDKQRFIGTAGAASTMMNP 60

Query: 61  RSTISQVKRLIGRNFSEPDVQIELKMFPFKTSEALDGSILVHVKYLGETHTFTPVQIMGM 120
           +++ISQ+KRLIGR FS+P++Q ++K  PF  +E  DG  L+H  YLGE   FTP Q+MGM
Sbjct: 61  KNSISQIKRLIGRQFSDPELQRDIKSLPFSVTEGPDGYPLIHANYLGEIRAFTPTQVMGM 120

Query: 121 LLAHLRDVAEKNLGAPFSDCVIGIPSYFTDLQRRLYWSAAVIAGLKPLRLMHDCTATALS 180
           +L++L+ +AEKNL     DC IGIP YFTDLQRR    AA IAGL PL L+H+ TATAL+
Sbjct: 121 MLSNLKGIAEKNLNTAVVDCCIGIPVYFTDLQRRAVLDAATIAGLHPLHLIHETTATALA 180

Query: 181 YGIYKTDFSNTGPIYVAFVDIGHCDTQVSIVSFEPGHMRIMSQTYDRDLGGRDFDEVLFS 240
           YGIYKTD      + VAF+DIGH   QV I  F+ G ++I+S  +DR LGGRDFDEVLF+
Sbjct: 181 YGIYKTDLPENDQLNVAFIDIGHASMQVCIAGFKKGQLKILSHAFDRSLGGRDFDEVLFN 240

Query: 241 HFVAEFKKNYDIDVNSNVKASIRLRAACEKLKKVLSANLEAVLNIECLMDEKDVKGFIKR 300
           HF A+FK  Y IDV+ N KAS+RLRA CEKLKKVLSAN  A LNIECLM EKDV+G IKR
Sbjct: 241 HFAAKFKDEYKIDVSQNAKASLRLRATCEKLKKVLSANPMAPLNIECLMAEKDVRGVIKR 300

Query: 301 EEFEKLASGLLEKISIPCTRGLADAGLAVENIHSVELVGSGSRIPAISRLLTSVFKKEPS 360
           EEFE+++  +LE++  P  + L+DAGL VE++H VE+VGSGSR+PA+ ++LT  F KEP 
Sbjct: 301 EEFEEISIPILERVKRPLEKALSDAGLTVEDVHMVEVVGSGSRVPAMIKILTEFFGKEPR 360

Query: 361 RKLNASECVARGCALQCAMLSPVFRVREYEIG---PFQ---------------GSNNSNS 420
           R +NASECV+RGCALQCA+LSP F+VRE+++    PF                G+ N  S
Sbjct: 361 RTMNASECVSRGCALQCAILSPTFKVREFQVHESFPFSISLAWKGAATDAQNGGTENQQS 420

Query: 421 RVKVRVQLNMNGIITVESATLVED---TIDQQMPRRDATYSNTEKMETEFVDSSHSESDA 480
            +   V    N I +V++ T       +ID Q       YS+   ++     S+++    
Sbjct: 421 TI---VFPKGNPIPSVKALTFYRSGTFSIDVQ-------YSDVNDLQAPPKISTYTIGPF 480

Query: 481 RKARGTR-RIDIPVSEHIYG--GMTKAELLEAQGRELQLAQQDKNMEQAK 507
           + ++G R ++ + V  +++G   +  A LLE +  E+ +  +D++ E AK
Sbjct: 481 QSSKGERAKLKVKVRLNLHGIVSVESATLLEEEEVEVSVT-KDQSEETAK 519

HSP 2 Score: 167.9 bits (424), Expect = 3.8e-40
Identity = 88/228 (38.60%), Postives = 141/228 (61.84%), Query Frame = 1

Query: 444 KMETEFVDSSHSESDARKARGTRRIDIPVSEHIYGGMTKAELLEAQGRELQLAQQDKNME 503
           +MET+    S +E+  +K + T   ++P+SE +YG +   E+ +A  +E ++A QD+ ME
Sbjct: 565 QMETD----SKAEAPKKKVKKT---NVPLSELVYGALKTVEVEKAVEKEFEMALQDRVME 624

Query: 504 QAKNKKNALESYVYEMRNKLFNTYRSFASDQEREGISSSLQQTEEWLYEDGDDETESAYS 563
           + K++KNA+ESYVY+MRNKL + Y+ + +D ERE   ++LQ+ E+WLYEDG+DET+  Y 
Sbjct: 625 ETKDRKNAVESYVYDMRNKLSDKYQEYITDSEREAFLANLQEVEDWLYEDGEDETKGVYV 684

Query: 564 SKLDGLKKLVDPIINRYEDEEARAQAKAHLLKRISDYRNSGDSLSPQV-------RALIF 623
           +KL+ LKK+ DP+  RY++   R      L   I+ YR +  S  P+        +  + 
Sbjct: 685 AKLEELKKVGDPVEVRYKESLERGSVIDQLGYCINSYREAAVSNDPKFDHIELAEKQKVL 744

Query: 624 EECDKVEQWLTEKNQQQELLAKNTDPLLWSSEIRTQEEDFDKTCQRIL 665
            EC + E WL EK QQQ+ L K   P L S++++++ E  DK C+ I+
Sbjct: 745 NECVEAEAWLREKQQQQDTLPKYATPALLSADVKSKAEALDKFCRPIM 785

HSP 3 Score: 58.9 bits (141), Expect = 2.5e-07
Identity = 77/333 (23.12%), Postives = 139/333 (41.74%), Query Frame = 1

Query: 385 RVREYEIGPFQGSNNSNSRVKVRVQLNMNGIITVESATLVEDTIDQQMPRRDATYSNTEK 444
           ++  Y IGPFQ S    +++KV+V+LN++GI++VESATL+E+         + + +  + 
Sbjct: 461 KISTYTIGPFQSSKGERAKLKVKVRLNLHGIVSVESATLLEE------EEVEVSVTKDQS 520

Query: 445 METEFVDSSHSESDARKARGTRRIDIPVSEHIYGGM-TKAELLEAQGRELQLAQQDKNME 504
            ET  +D+  + ++A  A G   +++  ++       T   + E+  + +Q+    K   
Sbjct: 521 EETAKMDTDKASAEAAPASGDSDVNMQDAKDTSDATGTDNGVPESAEKPVQMETDSKAEA 580

Query: 505 QAKNKKNA---LESYVY------EMRNKLFNTYRSFASDQEREGISSSLQQTEEWLYE-- 564
             K  K     L   VY      E+   +   +     D+  E         E ++Y+  
Sbjct: 581 PKKKVKKTNVPLSELVYGALKTVEVEKAVEKEFEMALQDRVMEETKDRKNAVESYVYDMR 640

Query: 565 ----DGDDE--TESAYSSKLDGLKKLVDPIINRYEDEEARAQ-AKAHLLKRISD-----Y 624
               D   E  T+S   + L  L+++ D +    EDE      AK   LK++ D     Y
Sbjct: 641 NKLSDKYQEYITDSEREAFLANLQEVEDWLYEDGEDETKGVYVAKLEELKKVGDPVEVRY 700

Query: 625 RNS----------GDSLSPQVRALIFEE--CDKVEQWLTEK----NQQQEL--------- 665
           + S          G  ++    A +  +   D +E  L EK    N+  E          
Sbjct: 701 KESLERGSVIDQLGYCINSYREAAVSNDPKFDHIE--LAEKQKVLNECVEAEAWLREKQQ 760

BLAST of Cucsa.053520.3 vs. Swiss-Prot
Match: HSP97_STRPU (97 kDa heat shock protein OS=Strongylocentrotus purpuratus PE=1 SV=2)

HSP 1 Score: 372.1 bits (954), Expect = 1.3e-101
Identity = 192/394 (48.73%), Postives = 262/394 (66.50%), Query Frame = 1

Query: 1   MSVVGFDIGNENCVIAVSRQRGIDVLLNEESQRETPAVIYFGEKQRFLGSAGAASATMNP 60
           MSVVGFD+GN +  IAV+R  GI+ + NE S R TP+V+ FGEK R  G A  + A  N 
Sbjct: 1   MSVVGFDVGNLSSYIAVARGGGIETMANEYSDRLTPSVVSFGEKSRTQGHAARSQAITNY 60

Query: 61  RSTISQVKRLIGRNFSEPDVQIELKMFPFKTSEALDGSILVHVKYLGETHTFTPVQIMGM 120
           ++T+SQ KR I R FS+P VQ + K+ P+K ++  +G++ + V+YLGET TFTP QI  M
Sbjct: 61  KNTLSQFKRFIARRFSDPSVQKDAKVVPYKITQLPNGNVGMQVQYLGETETFTPEQIYAM 120

Query: 121 LLAHLRDVAEKNLGAPFSDCVIGIPSYFTDLQRRLYWSAAVIAGLKPLRLMHDCTATALS 180
           +L  L+  AE NL     DCVI +P Y+TDL+RR    AA IAGL  LR++ D TA AL+
Sbjct: 121 ILTKLKSTAEINLCRKVVDCVISVPQYYTDLERRGVIHAAEIAGLNCLRVISDTTAVALA 180

Query: 181 YGIYKTDF--SNTGPIYVAFVDIGHCDTQVSIVSFEPGHMRIMSQTYDRDLGGRDFDEVL 240
           YGIYK D       P  V FVD GH   QVS+ +F  G +++++   D++LGGRDFD +L
Sbjct: 181 YGIYKQDLPTPEEKPRNVVFVDCGHSSLQVSVCAFNKGKLKVLANASDKNLGGRDFDWLL 240

Query: 241 FSHFVAEFKKNYDIDVNSNVKASIRLRAACEKLKKVLSANLEAV-LNIECLMDEKDVKGF 300
             HF  +F+  Y +DV SN +A +RL A C+K KK++SAN   + +NIEC+M+++DV G 
Sbjct: 241 AEHFAVDFQTRYKMDVKSNQRAWLRLMAECDKTKKLMSANATLISMNIECIMNDRDVSGK 300

Query: 301 IKREEFEKLASGLLEKISIPCTRGLADAGLAVENIHSVELVGSGSRIPAISRLLTSVFKK 360
           I R +FE LA+ LL+++ +P    L    L  E+IHS+E+VG  SRIP+I   +  VFKK
Sbjct: 301 ISRADFEALAAELLKRVEVPLKSVLEQTKLKPEDIHSIEIVGGSSRIPSIKETIKKVFKK 360

Query: 361 EPSRKLNASECVARGCALQCAMLSPVFRVREYEI 392
           E S  LN  E VARGCALQCA+LSP F+VR++ +
Sbjct: 361 ECSTTLNQDEAVARGCALQCAILSPTFKVRDFTV 394

HSP 2 Score: 114.4 bits (285), Expect = 5.0e-24
Identity = 72/242 (29.75%), Postives = 123/242 (50.83%), Query Frame = 1

Query: 440 SNTEKMETEFVDSSHSESDARKARGTRRIDIPVSEHIYGGMTKAELLEAQG---RELQLA 499
           S  +  E    D+S +E+DA+ A+ T++        I     +  + E      +E +L 
Sbjct: 581 SKDQNSEGSKSDNSSTETDAKAAKKTKKTIKTHELSITATTDELSITEVNNFFEKEGKLI 640

Query: 500 QQDKNMEQAKNKKNALESYVYEMRNKLFNTYRSFASDQEREGISSSLQQTEEWLYEDGDD 559
             D+  ++  + KNA+E YVYEMR KL + +  + S++ER   S  L++TE WLYEDG+D
Sbjct: 641 AHDRLEKEKNDAKNAVEEYVYEMREKLCDKFEQYISEKERGSFSKLLEETENWLYEDGED 700

Query: 560 ETESAYSSKLDGLKKLVDPIINRYEDEEAR-------AQAKAHLLKRISDYRNSGDSLSP 619
           ET+S Y +K++ LKK+ DP+ NR+++   R        +A    +K +  Y N  +  S 
Sbjct: 701 ETKSVYQTKINSLKKIGDPVENRFKENLERPGAFEDFGKALVPYIKTLDLYSNGDEKYSH 760

Query: 620 QVRALIFEECDKVEQ-------WLTEKNQQQELLAKNTDPLLWSSEIRTQEEDFDKTCQR 665
             +    E+  KVE+       W   K   Q   A + DP++ +++IR++ +     C  
Sbjct: 761 IEK----EDMAKVEKCVKEKVAWRDSKVNAQNQKAPHQDPVVTAAQIRSEIQSMKFVCDP 818

HSP 3 Score: 48.1 bits (113), Expect = 4.4e-04
Identity = 65/303 (21.45%), Postives = 122/303 (40.26%), Query Frame = 1

Query: 382 PVFRVREYEI-GPFQGSNNSNSRVKVRVQLNMNGIITVESATLVED-TIDQQMPRRDATY 441
           P  R+  ++I G F  +   +S++KV+V+++ +GI  V SA+L+E   +  +    D + 
Sbjct: 453 PERRIGRFKINGVFPTTEGESSKIKVKVRVDGHGIFNVASASLIEKLPVQAEDAMEDGSP 512

Query: 442 SNTEKMETEFVDSSHSESDARKARGTRRIDIPVSEHIYGGMTKAELLEAQGRELQLAQQD 501
                 + E   +S +E+DA   +       PV      G   A+  E      +   +D
Sbjct: 513 EENGPSKEEGSGASQAENDAPMDQS------PVQGGAGEGEASADKEEQADNGSKETSKD 572

Query: 502 KNMEQAKNKKNALESYVYEMRNKLFNTYRSFASDQEREGISSSLQQTEEWLYEDGDDETE 561
              + +++ K+  ES                  DQ  EG  S    TE         +T+
Sbjct: 573 SKDQTSESSKSDKES-----------------KDQNSEGSKSDNSSTE--TDAKAAKKTK 632

Query: 562 SAYSSKLDGLKKLVDPI----INRYEDEEARAQAKAHLLKRISDYRNSGDSLSPQVRALI 621
               +    +    D +    +N + ++E +  A   L K  +D +N+ +    ++R  +
Sbjct: 633 KTIKTHELSITATTDELSITEVNNFFEKEGKLIAHDRLEKEKNDAKNAVEEYVYEMREKL 692

Query: 622 FEECDKVEQWLTEKNQQQELLAKNTDPLLWSSEIRTQEEDFDKTCQRILGPVSSHTNSGD 679
              CDK EQ+++EK +       +   LL  +E    E+  D+T       ++S    GD
Sbjct: 693 ---CDKFEQYISEKER------GSFSKLLEETENWLYEDGEDETKSVYQTKINSLKKIGD 721

BLAST of Cucsa.053520.3 vs. Swiss-Prot
Match: HSP97_STRFN (97 kDa heat shock protein OS=Strongylocentrotus franciscanus GN=HSP110 PE=2 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 2.3e-101
Identity = 192/394 (48.73%), Postives = 261/394 (66.24%), Query Frame = 1

Query: 1   MSVVGFDIGNENCVIAVSRQRGIDVLLNEESQRETPAVIYFGEKQRFLGSAGAASATMNP 60
           MSVVGFD+GN +  IAV+R  GI+ + NE S R TP+V+ FGEK R  G A  + A  N 
Sbjct: 1   MSVVGFDVGNLSSYIAVARGGGIETMANEYSDRLTPSVVSFGEKSRTQGHAARSQAITNY 60

Query: 61  RSTISQVKRLIGRNFSEPDVQIELKMFPFKTSEALDGSILVHVKYLGETHTFTPVQIMGM 120
           ++T+SQ KR I R FS+P VQ +  + P+K ++  +G++ + V+YLGET TFTP QI  M
Sbjct: 61  KNTLSQFKRFIARQFSDPSVQKDAHVVPYKVTQLPNGNVGMQVQYLGETETFTPEQIYAM 120

Query: 121 LLAHLRDVAEKNLGAPFSDCVIGIPSYFTDLQRRLYWSAAVIAGLKPLRLMHDCTATALS 180
           +L  L+  AE NL     DCVI +P Y+TDL+RR    AA IAGL  LR++ D TA AL+
Sbjct: 121 ILTKLKATAEVNLCRKVVDCVISVPQYYTDLERRGVIHAAEIAGLNCLRVISDTTAVALA 180

Query: 181 YGIYKTDF--SNTGPIYVAFVDIGHCDTQVSIVSFEPGHMRIMSQTYDRDLGGRDFDEVL 240
           YGIYK D       P  V FVD GH   QVS+ +F  G +++++   D++LGGRDFD +L
Sbjct: 181 YGIYKQDLPTPEEKPRNVVFVDCGHSSLQVSVCAFNKGKLKVLANASDKNLGGRDFDWLL 240

Query: 241 FSHFVAEFKKNYDIDVNSNVKASIRLRAACEKLKKVLSANLEAV-LNIECLMDEKDVKGF 300
             HF  +F+  Y +DV SN +A +RL A C+K KK++SAN   + +NIEC+M+++DV G 
Sbjct: 241 AEHFAVDFQTRYKMDVKSNQRAWLRLMAECDKTKKLMSANATVISMNIECIMNDRDVSGK 300

Query: 301 IKREEFEKLASGLLEKISIPCTRGLADAGLAVENIHSVELVGSGSRIPAISRLLTSVFKK 360
           I R +FE LA+ LL+++ +P    L    L  E+IHS+E+VG  SRIP+I   +  VFKK
Sbjct: 301 ISRADFEALAAELLKRVEVPLKSVLEQTKLKPEDIHSIEIVGGSSRIPSIKETIKKVFKK 360

Query: 361 EPSRKLNASECVARGCALQCAMLSPVFRVREYEI 392
           E S  LN  E VARGCALQCA+LSP FRVR++ +
Sbjct: 361 ECSTTLNQDEAVARGCALQCAILSPTFRVRDFTV 394

HSP 2 Score: 113.2 bits (282), Expect = 1.1e-23
Identity = 70/238 (29.41%), Postives = 116/238 (48.74%), Query Frame = 1

Query: 440 SNTEKMETEFVDSSHSESDARKARGTRRIDIPVSEHIYGGMTKAELLEAQG---RELQLA 499
           S  +  E    D+  +E+DA+  +  ++        I     +  + E      +E ++ 
Sbjct: 580 SKDQNSEGSKSDNGSTETDAKATKKNKKTIKTHELTITASTDELSIAEVNNFFEKEGKMI 639

Query: 500 QQDKNMEQAKNKKNALESYVYEMRNKLFNTYRSFASDQEREGISSSLQQTEEWLYEDGDD 559
            QD+  ++  + KNA+E YVY+MR KL + +  + S++ER   S  L++TE WLYEDG+D
Sbjct: 640 AQDRLEKEKNDAKNAVEEYVYDMREKLCDKFEQYVSEKERGSFSKLLEETENWLYEDGED 699

Query: 560 ETESAYSSKLDGLKKLVDPIINRYEDEEARAQAKAHLLKRISDYRNSGDSLSPQVRALIF 619
           ET+S Y +K+  LKK+ DPI NRY+++  R  A   L K +  Y  + D  S        
Sbjct: 700 ETKSVYQAKITSLKKIGDPIENRYKEKHERPVAFEELGKALMLYGKTLDLYSQGDEKYSH 759

Query: 620 EECDKV----------EQWLTEKNQQQELLAKNTDPLLWSSEIRTQEEDFDKTCQRIL 665
            E D++          E W   K   Q   A   DP++ + +IR++ +     C  I+
Sbjct: 760 IEKDEMAKVEKCLKEKEAWRDSKTSAQNQKAAYQDPVVTAQQIRSEIQSMKFICDPII 817

HSP 3 Score: 49.3 bits (116), Expect = 2.0e-04
Identity = 85/366 (23.22%), Postives = 151/366 (41.26%), Query Frame = 1

Query: 334 SVELVGSGSRIPAISRLLTSVFKKEPSRKLNASECVARGCALQCAMLSPVFRVREYEI-G 393
           S+E+     + P  S++LT  ++KEP       E VAR       +  P  R+  ++I G
Sbjct: 415 SMEVSHKNHQAP-FSKMLT-FYRKEPF------ELVARYADTNLPL--PERRIGRFKING 474

Query: 394 PFQGSNNSNSRVKVRVQLNMNGIITVESATLVEDTIDQQMPRRDATYSNTEKMETEFVDS 453
            F  +   +S++KV+V+++ +GI  V SA+L+E     ++P          + E    DS
Sbjct: 475 VFPTAEGESSKIKVKVRVDGHGIFKVSSASLIE-----KLP---------AQAEDAMEDS 534

Query: 454 SHSESDARKARGT----RRIDIPVSEH-IYGGMTKAELLEAQGRELQLAQQDKNMEQAKN 513
           S  E+   K  G+       D P+ +  + GG  + E   +  +E Q     K  E +K+
Sbjct: 535 SPEENGPSKEEGSGASQSENDAPMDQSPVEGGAGEGE--ASADKEEQAENGAK--ETSKD 594

Query: 514 KKNALESYVYEMRNKLFNTYRSFASDQEREGISSSLQQTEEWLYEDGDDETESAYSSK-- 573
           K    E    +  +K          DQ  EG  S          ++G  ET++  + K  
Sbjct: 595 KDQTSEGSKSDKESK----------DQNSEGSKS----------DNGSTETDAKATKKNK 654

Query: 574 ----------LDGLKKLVDPIINRYEDEEARAQAKAHLLKRISDYRNSGDSLSPQVRALI 633
                          +L    +N + ++E +  A+  L K  +D +N+ +     +R  +
Sbjct: 655 KTIKTHELTITASTDELSIAEVNNFFEKEGKMIAQDRLEKEKNDAKNAVEEYVYDMREKL 714

Query: 634 FEECDKVEQWLTEKNQQQELLAKNTDPLLWSSEIRTQEEDFDKTCQRILGPVSSHTNSGD 682
              CDK EQ+++EK +       +   LL  +E    E+  D+T       ++S    GD
Sbjct: 715 ---CDKFEQYVSEKER------GSFSKLLEETENWLYEDGEDETKSVYQAKITSLKKIGD 723

BLAST of Cucsa.053520.3 vs. TrEMBL
Match: A0A0D2QQV9_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_004G027900 PE=3 SV=1)

HSP 1 Score: 918.3 bits (2372), Expect = 5.6e-264
Identity = 462/676 (68.34%), Postives = 540/676 (79.88%), Query Frame = 1

Query: 1   MSVVGFDIGNENCVIAVSRQRGIDVLLNEESQRETPAVIYFGEKQRFLGSAGAASATMNP 60
           MSVVGFDIGNE CVIA  +QRG+DVLLN+ES+RETPA++ FGEKQRFLGSA AASA M+P
Sbjct: 1   MSVVGFDIGNEKCVIAAVKQRGVDVLLNDESKRETPALVCFGEKQRFLGSAAAASAMMHP 60

Query: 61  RSTISQVKRLIGRNFSEPDVQIELKMFPFKTSEALDGSILVHVKYLGETHTFTPVQIMGM 120
           ++T+SQVKRLIGR F +PDVQ EL+M PF+TSE  DG IL+H+KYLGETH FTPVQIM M
Sbjct: 61  KTTVSQVKRLIGRKFQDPDVQNELRMLPFETSEGQDGGILIHLKYLGETHRFTPVQIMAM 120

Query: 121 LLAHLRDVAEKNLGAPFSDCVIGIPSYFTDLQRRLYWSAAVIAGLKPLRLMHDCTATALS 180
           L AHL+ + E NLG P  DCVIGIPSYFTDLQRR Y  AA IAGLKPLRLMHDCTATAL 
Sbjct: 121 LFAHLKYMTETNLGLPVLDCVIGIPSYFTDLQRRAYLDAAKIAGLKPLRLMHDCTATALG 180

Query: 181 YGIYKTDFSNTGPIYVAFVDIGHCDTQVSIVSFEPGHMRIMSQTYDRDLGGRDFDEVLFS 240
           YGIYKTDFSN GP  VAFVDIGHCDTQVSIVSFE G MRI+S  +D  LGGRDFDE+LF 
Sbjct: 181 YGIYKTDFSNAGPTNVAFVDIGHCDTQVSIVSFEAGQMRILSHAFDNSLGGRDFDEILFG 240

Query: 241 HFVAEFKKNYDIDVNSNVKASIRLRAACEKLKKVLSANLEAVLNIECLMDEKDVKGFIKR 300
           +F A FK+ Y+IDV SNV+ASIRLRAACEKLKKVLSAN EA LNIECLMDEKDVKGFIKR
Sbjct: 241 YFAACFKEQYNIDVYSNVRASIRLRAACEKLKKVLSANAEAPLNIECLMDEKDVKGFIKR 300

Query: 301 EEFEKLASGLLEKISIPCTRGLADAGLAVENIHSVELVGSGSRIPAISRLLTSVFKKEPS 360
           EEFEKLASGLLE+I+IPCT+ + DAGL V  I +VELVGSGSRIPAI+R L S F++EPS
Sbjct: 301 EEFEKLASGLLERINIPCTKAITDAGLTVGKILAVELVGSGSRIPAITRQLASFFRREPS 360

Query: 361 RKLNASECVARGCALQCAMLSPVFRVREYEIGPFQGSNNSNSRVKVRVQLNMNGIITVES 420
           R +NASECVARGCALQCAMLSP+FRVR+YEIGPFQ SN+  +RVKV+VQLN++GI+ VES
Sbjct: 361 RTINASECVARGCALQCAMLSPIFRVRDYEIGPFQASNSERARVKVKVQLNLHGIVNVES 420

Query: 421 ATLVEDTI-DQQMPRRDATYSNTEKMETEFVDSSHSESDA---RKARGTRRIDIPVSEHI 480
           A L+E+ + D ++  ++  +      +  ++    S + A      +  RR++IPVSE+I
Sbjct: 421 AMLIEEHVDDSELSTKEVRHVTNGSEDGTYMQPKPSNASADGKTNDKTMRRLEIPVSENI 480

Query: 481 YGGMTKAELLEAQGRELQLAQQDKNMEQAKNKKNALESYVYEMRNKLFNTYRSFASDQER 540
            G MT  EL+EAQ +EL+LAQQD+ MEQ K KKNALESYVYEMRNKLFN YR FASD+ER
Sbjct: 481 NGTMTGVELMEAQDKELKLAQQDRTMEQTKEKKNALESYVYEMRNKLFNIYRGFASDEER 540

Query: 541 EGISSSLQQTEEWLYEDGDDETESAYSSKLDGLKKLVDPIINRYEDEEARAQAKAHLLKR 600
           EGIS SLQ+TEEWLY+DG+DETE AY+SKL+ LKKLVDP+ NR++DEEAR QA   L K 
Sbjct: 541 EGISKSLQETEEWLYDDGEDETEGAYTSKLEALKKLVDPVENRFKDEEARTQASTDLFKC 600

Query: 601 ISDYRNSGDSLSPQVRALIFEECDKVEQWLTEKNQQQELLAKNTDPLLWSSEIRTQEEDF 660
           I+D R S  SL  + R  I  EC+K EQWLTEK +QQ  L KN DPLLWSSEI+   ED 
Sbjct: 601 IADIRMSTKSLPNEDRESILNECNKAEQWLTEKTKQQHSLPKNIDPLLWSSEIKKMTEDL 660

Query: 661 DKTCQRILGPVSSHTN 673
           +  C  I+   +SH N
Sbjct: 661 NMKCMHIMSRKASHRN 676

BLAST of Cucsa.053520.3 vs. TrEMBL
Match: A0A0A0L803_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G391900 PE=3 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 1.1e-219
Identity = 389/391 (99.49%), Postives = 391/391 (100.00%), Query Frame = 1

Query: 1   MSVVGFDIGNENCVIAVSRQRGIDVLLNEESQRETPAVIYFGEKQRFLGSAGAASATMNP 60
           MSVVGFDIGNENCVIAVSRQRGIDVLLNEESQRETPAVIYFGEKQRFLGSAGAASATMNP
Sbjct: 1   MSVVGFDIGNENCVIAVSRQRGIDVLLNEESQRETPAVIYFGEKQRFLGSAGAASATMNP 60

Query: 61  RSTISQVKRLIGRNFSEPDVQIELKMFPFKTSEALDGSILVHVKYLGETHTFTPVQIMGM 120
           RSTISQVKRLIGRNFSEPDVQIELKMFPFKTSEALDGSILVHVKYLGETHTFTPVQIMGM
Sbjct: 61  RSTISQVKRLIGRNFSEPDVQIELKMFPFKTSEALDGSILVHVKYLGETHTFTPVQIMGM 120

Query: 121 LLAHLRDVAEKNLGAPFSDCVIGIPSYFTDLQRRLYWSAAVIAGLKPLRLMHDCTATALS 180
           LLAHL+DVAEKNLGAPFSDCVIGIPSYFTDLQRRLYWSAAVIAGLKPLRLMHDCTATALS
Sbjct: 121 LLAHLKDVAEKNLGAPFSDCVIGIPSYFTDLQRRLYWSAAVIAGLKPLRLMHDCTATALS 180

Query: 181 YGIYKTDFSNTGPIYVAFVDIGHCDTQVSIVSFEPGHMRIMSQTYDRDLGGRDFDEVLFS 240
           YGIYKTDFSNTGPIYVAFVDIGHCDTQVSIVSFEPGHMRIMSQTYDRDLGGRDFDEVLFS
Sbjct: 181 YGIYKTDFSNTGPIYVAFVDIGHCDTQVSIVSFEPGHMRIMSQTYDRDLGGRDFDEVLFS 240

Query: 241 HFVAEFKKNYDIDVNSNVKASIRLRAACEKLKKVLSANLEAVLNIECLMDEKDVKGFIKR 300
           HFVAEFKKNYDIDVNSNVKASIRLRAACEKLKKVLSANLEAVLNIECLMDEKDVKGFIKR
Sbjct: 241 HFVAEFKKNYDIDVNSNVKASIRLRAACEKLKKVLSANLEAVLNIECLMDEKDVKGFIKR 300

Query: 301 EEFEKLASGLLEKISIPCTRGLADAGLAVENIHSVELVGSGSRIPAISRLLTSVFKKEPS 360
           EEFEKLASGLLEKISIPCTRGLADAGLAVENIHSVELVGSGSRIPAISRLLTSVFKKEPS
Sbjct: 301 EEFEKLASGLLEKISIPCTRGLADAGLAVENIHSVELVGSGSRIPAISRLLTSVFKKEPS 360

Query: 361 RKLNASECVARGCALQCAMLSPVFRVREYEI 392
           RKLNASECVARGCALQCAMLSPVFRVREYE+
Sbjct: 361 RKLNASECVARGCALQCAMLSPVFRVREYEV 391

BLAST of Cucsa.053520.3 vs. TrEMBL
Match: A0A0A0L803_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G391900 PE=3 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 1.8e-166
Identity = 299/303 (98.68%), Postives = 301/303 (99.34%), Query Frame = 1

Query: 389 YEIGPFQGSNNSNSRVKVRVQLNMNGIITVESATLVEDTIDQQMPRRDATYSNTEKMETE 448
           + IGPFQGSNNSNSRVKVRVQLNMNGIITVESATLVEDTIDQQMPRRDATYSNTEKMETE
Sbjct: 460 FTIGPFQGSNNSNSRVKVRVQLNMNGIITVESATLVEDTIDQQMPRRDATYSNTEKMETE 519

Query: 449 FVDSSHSESD-ARKARGTRRIDIPVSEHIYGGMTKAELLEAQGRELQLAQQDKNMEQAKN 508
           FVDSSHSESD +RKARGTRRIDIPVSEHIYGGMTKAELLEAQGRELQLAQQDKNMEQAKN
Sbjct: 520 FVDSSHSESDVSRKARGTRRIDIPVSEHIYGGMTKAELLEAQGRELQLAQQDKNMEQAKN 579

Query: 509 KKNALESYVYEMRNKLFNTYRSFASDQEREGISSSLQQTEEWLYEDGDDETESAYSSKLD 568
           KKNALESYVYEMRNKLFNTYRSFASDQEREGISSSLQQTEEWLYEDGDDETESAYSSKLD
Sbjct: 580 KKNALESYVYEMRNKLFNTYRSFASDQEREGISSSLQQTEEWLYEDGDDETESAYSSKLD 639

Query: 569 GLKKLVDPIINRYEDEEARAQAKAHLLKRISDYRNSGDSLSPQVRALIFEECDKVEQWLT 628
           GLKKLVDPIINRYEDEEARAQAKAHLLKRISDYRNSGDSLSPQVRALIFEECDKVEQWLT
Sbjct: 640 GLKKLVDPIINRYEDEEARAQAKAHLLKRISDYRNSGDSLSPQVRALIFEECDKVEQWLT 699

Query: 629 EKNQQQELLAKNTDPLLWSSEIRTQEEDFDKTCQRILGPVSSHTNSGDSKETNHHNSSDN 688
           EKNQQQELLAKNTDPLLWSSEIRTQEEDFDKTCQRILGPVSSHTNSGDSKETNHHNSSDN
Sbjct: 700 EKNQQQELLAKNTDPLLWSSEIRTQEEDFDKTCQRILGPVSSHTNSGDSKETNHHNSSDN 759

Query: 689 HHS 691
           HHS
Sbjct: 760 HHS 762

HSP 2 Score: 656.4 bits (1692), Expect = 3.9e-185
Identity = 355/784 (45.28%), Postives = 496/784 (63.27%), Query Frame = 1

Query: 1   MSVVGFDIGNENCVIAVSRQRGIDVLLNEESQRETPAVIYFGEKQRFLGSAGAASATMNP 60
           MSVVGFD GNE+ V+AV+RQRGIDV+LN+ES+RETPA++ FGEKQRFLG+AGAAS+ MNP
Sbjct: 1   MSVVGFDFGNESGVVAVARQRGIDVVLNDESKRETPAIVCFGEKQRFLGTAGAASSMMNP 60

Query: 61  RSTISQVKRLIGRNFSEPDVQIELKMFPFKTSEALDGSILVHVKYLGETHTFTPVQIMGM 120
           ++TISQ+KRLIGR FS+P++Q ++K  PF  +E  DG  L++ +YLGE  +FTP Q++GM
Sbjct: 61  KNTISQIKRLIGRQFSDPELQRDIKSLPFSVTEGPDGYPLINAQYLGEMRSFTPTQVLGM 120

Query: 121 LLAHLRDVAEKNLGAPFSDCVIGIPSYFTDLQRRLYWSAAVIAGLKPLRLMHDCTATALS 180
           + + L+ +AEKNL A   DC IGIP YFTDLQRR    AA IAGL PLRL+H+ TATAL+
Sbjct: 121 VFSDLKSIAEKNLNAAVVDCCIGIPIYFTDLQRRAVLDAATIAGLHPLRLIHETTATALA 180

Query: 181 YGIYKTDFSNTGPIYVAFVDIGHCDTQVSIVSFEPGHMRIMSQTYDRDLGGRDFDEVLFS 240
           YGIYKTD      + VAFVDIGH   QV I   + G ++I++  +DR LGGRDFDEVLF 
Sbjct: 181 YGIYKTDLPENDQLNVAFVDIGHASMQVCIAGLKKGQLKILAHAFDRSLGGRDFDEVLFQ 240

Query: 241 HFVAEFKKNYDIDVNSNVKASIRLRAACEKLKKVLSANLEAVLNIECLMDEKDVKGFIKR 300
           +F  +FK  Y IDV  N +AS+RLRAACEKLKKVLSAN EA LNIECLMDEKDV+GFIKR
Sbjct: 241 NFAEKFKTEYKIDVYQNARASLRLRAACEKLKKVLSANPEAPLNIECLMDEKDVRGFIKR 300

Query: 301 EEFEKLASGLLEKISIPCTRGLADAGLAVENIHSVELVGSGSRIPAISRLLTSVFKKEPS 360
           +EFE+++  +LE++  P  + L++AGL VENI++VE+VGSGSR+PAI ++LT  F KEP 
Sbjct: 301 DEFEQISIPILERVKKPLEKALSEAGLTVENIYAVEVVGSGSRVPAIMKILTEFFGKEPR 360

Query: 361 RKLNASECVARGCALQ-------------------------------------------- 420
           R +NASECVA+GCAL+                                            
Sbjct: 361 RTMNASECVAKGCALECAILSPTFKVREFQVNESFPFTIALSWKGTAPDAQNGADNQQID 420

Query: 421 --CAMLSPV---FRVREYEIGPFQGSNNSNSRVKVRVQLNMNGIITVESATLVEDTID-- 480
              A ++ V     +  Y IGPFQ + +  ++VKV+V+LN++G++++ESATL+E+ ++  
Sbjct: 421 VHYADVNEVQAPAMISTYTIGPFQSTKSERAKVKVKVRLNLHGVVSIESATLLEEDVEIP 480

Query: 481 --QQMPRRDATYS-----------NTEKMETEFVDSSHSESDARKARG------------ 540
             ++  + DA  S           N   ME     ++++   A  A+G            
Sbjct: 481 VVKETAKMDADESPPVPAPSSGAENDVDMEDAKSTAANAPGSANGAQGDVPVQMETESKV 540

Query: 541 ------TRRIDIPVSEHIYGGMTKAELLEAQGRELQLAQQDKNMEQAKNKKNALESYVYE 600
                  ++ DIPV+E +YGGM   ++ +A  +E ++A QD+ ME+ K+KKNA+E+YVY+
Sbjct: 541 EVPKKKVKKTDIPVTELVYGGMQPTDVQKAVEKEFEMALQDRVMEETKDKKNAVEAYVYD 600

Query: 601 MRNKLFNTYRSFASDQEREGISSSLQQTEEWLYEDGDDETESAYSSKLDGLKKLVDPIIN 660
           MRNKL + Y  F +D ERE  ++ LQQ E+WLYEDG+DET+  Y +KL+ LKK  DP+  
Sbjct: 601 MRNKLHDKYHDFVTDPEREEFTAILQQVEDWLYEDGEDETKGVYVAKLEELKKRGDPVEL 660

Query: 661 RYEDEEARAQAKAHLLKRISDYR-------NSGDSLSPQVRALIFEECDKVEQWLTEKNQ 687
           RY++   R      L+  I+ YR       +  D +    +  + +EC   E WL EK Q
Sbjct: 661 RYKEHLERGSVVDQLVYCINSYREAVMSPDSKFDHIDIAEKEKVLKECADAEAWLREKKQ 720

BLAST of Cucsa.053520.3 vs. TrEMBL
Match: M5X6Z0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001697mg PE=3 SV=1)

HSP 1 Score: 637.5 bits (1643), Expect = 1.9e-179
Identity = 311/391 (79.54%), Postives = 348/391 (89.00%), Query Frame = 1

Query: 1   MSVVGFDIGNENCVIAVSRQRGIDVLLNEESQRETPAVIYFGEKQRFLGSAGAASATMNP 60
           MSVVGFDIGNENCVIAV +QRG+DVLLN+ES+RETPAV+ FGEKQRFLGSAGAASA MNP
Sbjct: 1   MSVVGFDIGNENCVIAVVKQRGVDVLLNDESKRETPAVVCFGEKQRFLGSAGAASAMMNP 60

Query: 61  RSTISQVKRLIGRNFSEPDVQIELKMFPFKTSEALDGSILVHVKYLGETHTFTPVQIMGM 120
           +ST+SQVKRLIGR FSEPDVQ +L+M PF+TSEA DG IL+H+KYLGETHTFTPVQ+  M
Sbjct: 61  KSTVSQVKRLIGRKFSEPDVQRDLRMLPFQTSEAPDGGILIHLKYLGETHTFTPVQVTAM 120

Query: 121 LLAHLRDVAEKNLGAPFSDCVIGIPSYFTDLQRRLYWSAAVIAGLKPLRLMHDCTATALS 180
           L AHL+D+ EKN   P SDCVIGIPSYFTDLQRR Y  AA +AGLKPLRLMHDCTATALS
Sbjct: 121 LFAHLKDLIEKNQEMPISDCVIGIPSYFTDLQRRAYLDAATVAGLKPLRLMHDCTATALS 180

Query: 181 YGIYKTDFSNTGPIYVAFVDIGHCDTQVSIVSFEPGHMRIMSQTYDRDLGGRDFDEVLFS 240
           YGIYK+DF ++GP YVAFVDIGHCDTQV+I SFE G M+I+S T++R LGGRDFDE+LF 
Sbjct: 181 YGIYKSDFPSSGPTYVAFVDIGHCDTQVTIASFEAGQMKILSHTFERSLGGRDFDEILFG 240

Query: 241 HFVAEFKKNYDIDVNSNVKASIRLRAACEKLKKVLSANLEAVLNIECLMDEKDVKGFIKR 300
           HF A+FK+ Y IDV SNVKASIRLRAACEKLKKVLSAN EA LNIECLMDEKDVKGFIKR
Sbjct: 241 HFAAQFKEQYRIDVYSNVKASIRLRAACEKLKKVLSANAEAPLNIECLMDEKDVKGFIKR 300

Query: 301 EEFEKLASGLLEKISIPCTRGLADAGLAVENIHSVELVGSGSRIPAISRLLTSVFKKEPS 360
           E+FE L+SGLLE+I +PC++ LADAGL  E IHSVELVGSGSRIPA+ R+LTSVF+KEP 
Sbjct: 301 EDFEMLSSGLLERIGVPCSKALADAGLTAEKIHSVELVGSGSRIPAVGRVLTSVFRKEPR 360

Query: 361 RKLNASECVARGCALQCAMLSPVFRVREYEI 392
           R LNASECVARGCALQCAMLSPVFRVREYE+
Sbjct: 361 RTLNASECVARGCALQCAMLSPVFRVREYEV 391

BLAST of Cucsa.053520.3 vs. TrEMBL
Match: M5X6Z0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001697mg PE=3 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 7.1e-86
Identity = 175/319 (54.86%), Postives = 227/319 (71.16%), Query Frame = 1

Query: 389 YEIGPFQGSNNSNSRVKVRVQLNMNGIITVESATLVEDTIDQQMPRRDA----------- 448
           ++IGPFQ S++  +RVKV++QL++NG++ VESA ++E+  D    R  A           
Sbjct: 460 FKIGPFQCSHSEKTRVKVKIQLDLNGVVFVESAMMMEEHGDDSSTRGVADSMDPMDIDCV 519

Query: 449 -TYSNTEKMETEFVDSSHSESDARKARG--------TRRIDIPVSEHIYGGMTKAELLEA 508
               ++E +   F +SS  +S +  A G        TRR++IPV+E+IYGGMTKAEL EA
Sbjct: 520 TASGSSEAVGDGFQESSSMQSKSSHASGDGKRDNKSTRRLEIPVTENIYGGMTKAELSEA 579

Query: 509 QGRELQLAQQDKNMEQAKNKKNALESYVYEMRNKLFNTYRSFASDQEREGISSSLQQTEE 568
           Q +ELQL QQD+ MEQ K+KKNALESYVY+MRNKLFNTYRSFASDQEREGIS SLQQTEE
Sbjct: 580 QEKELQLGQQDRIMEQTKDKKNALESYVYDMRNKLFNTYRSFASDQEREGISRSLQQTEE 639

Query: 569 WLYEDGDDETESAYSSKLDGLKKLVDPIINRYEDEEARAQAKAHLLKRISDYRNSGDSLS 628
           WLY+DG+DETE+AY+SKL+ LKK+VDPI NRY+DEEAR QA   LLK I DYR + +SL 
Sbjct: 640 WLYDDGEDETENAYTSKLEDLKKMVDPIENRYKDEEARLQATRDLLKCIGDYRMAVNSLP 699

Query: 629 PQVRALIFEECDKVEQWLTEKNQQQELLAKNTDPLLWSSEIRTQEEDFDKTCQRILGPVS 688
           P  R  I  EC KVEQWL EKNQ Q+ L KN DP+LWSS+I+++ E+ +  C+ +    S
Sbjct: 700 PMDRESIVNECYKVEQWLREKNQLQDSLPKNVDPVLWSSDIKSRAEELNSRCKHMFR--S 759

HSP 2 Score: 625.5 bits (1612), Expect = 7.4e-176
Identity = 349/734 (47.55%), Postives = 462/734 (62.94%), Query Frame = 1

Query: 1   MSVVGFDIGNENCVIAVSRQRGIDVLLNEESQRETPAVIYFGEKQRFLGSAGAASATMNP 60
           MSVVGFD+GN+  V A +RQRGIDVLLN ES RE+PA I F    R LG   A+S     
Sbjct: 1   MSVVGFDVGNDTLVAAAARQRGIDVLLNAESNRESPAAIAFSHNARLLGPHAASS----- 60

Query: 61  RSTISQVKRLIGRNFSEPDVQIELKMFPFKTSEAL-DGS--ILVHVKYLGETHTFTPVQI 120
            +  S VKRL+           +L   PF       DG   +LVHV ++G     +P Q+
Sbjct: 61  HAPFSSVKRLLR------GATADLSRLPFPVEVVSPDGGRGVLVHVDHIGRRIALSPTQL 120

Query: 121 MGMLLAHLRDVAEKNLGAPFSDCVIGIPSYFTDLQRRLYWSAAVIAGLKPLRLMHDCTAT 180
           + MLL +LR +AE +L AP ++CVI +P YFT  QR+ Y  AA +AGL+PLRLMHD  AT
Sbjct: 121 LAMLLGYLRQLAEADLEAPVAECVISVPCYFTQAQRQAYLDAAAVAGLRPLRLMHDLAAT 180

Query: 181 ALSYGIYKTDFSNTG-PIYVAFVDIGHCDTQVSIVSFEPGHMRIMSQTYDRDLGGRDFDE 240
           AL YG+Y++D    G P YVAFVD+GHCDTQV++V+F+   M+++S  +D DLGGRDFDE
Sbjct: 181 ALGYGLYRSDLGGPGGPTYVAFVDVGHCDTQVAVVAFDVSGMKVLSHGFDADLGGRDFDE 240

Query: 241 VLFSHFVAEFKKNYDIDVNSNVKASIRLRAACEKLKKVLSANLEAVLNIECLMDEKDVKG 300
           VLF HF  EF+  Y IDV  NVKAS+RLRAACEK KKVLSAN EAV+NIECLM+EKDV+G
Sbjct: 241 VLFEHFAEEFRDRYKIDVTGNVKASMRLRAACEKAKKVLSANAEAVVNIECLMEEKDVRG 300

Query: 301 FIKREEFEKLASGLLEKISIPCTRGLADAGLAVENIHSVELVGSGSRIPAISRLLTSVFK 360
            I+REEFEKL +GLLE++  PC + +  + +  + +HSVELVGSGSR+PAI+R+L   F+
Sbjct: 301 MIRREEFEKLCAGLLERVVEPCKKAMEGSRIGFDKLHSVELVGSGSRVPAIARILAGFFR 360

Query: 361 KEPSRKLNASECVARGCA----------------LQCAMLSPV-FRVREYEIGPFQGS-- 420
           +EPSR LNASECVARGCA                +Q A+ + + F   E  I     +  
Sbjct: 361 REPSRTLNASECVARGCALQCAMLSPTFRVREYEVQDAISASIGFCTSEGPISTLPSNAL 420

Query: 421 ---NNSNSRVKVRVQLNMNGIITVESATLVEDTIDQQMPRRDATY------SNTEKMETE 480
               +    VKV V L+ N    +++  + E+ +      +  T+      ++TEK + +
Sbjct: 421 FQRGHPLPSVKV-VTLHKNNKFKLDAFYVDENELPPGASTKIGTFQIGPFQAHTEKSKVK 480

Query: 481 FVDSSHSESDAR----------------KARGTRRIDIPVSEHIYGGMTKAELLEAQGRE 540
            +D   S++ +                 K+R  R I +P+   IYG M+  ELLEAQ +E
Sbjct: 481 LIDDDQSDAHSADSMEVDSNGEMGQQGDKSRSERLIQLPIIPSIYGAMSSQELLEAQEQE 540

Query: 541 LQLAQQDKNMEQAKNKKNALESYVYEMRNKLFNTYRSFASDQEREGISSSLQQTEEWLYE 600
            QLA QDK ME+ K++KNALESYVY+ RNKL   YRSFA+D ERE IS SLQQTE+WLYE
Sbjct: 541 SQLAYQDKLMERTKDRKNALESYVYDTRNKLSERYRSFATDSEREEISLSLQQTEDWLYE 600

Query: 601 DGDDETESAYSSKLDGLKKLVDPIINRYEDEEARAQAKAHLLKRISDYRNSGDSLSPQVR 660
           +GDDETE+ Y+SKL+ LK+LVDPI NR +DEE RAQA   LLK I D++ +  SL    +
Sbjct: 601 EGDDETEAVYNSKLEELKRLVDPIENRCKDEEVRAQATRDLLKCIVDHKMAAKSLPTPEQ 660

Query: 661 ALIFEECDKVEQWLTEKNQQQELLAKNTDPLLWSSEIRTQEEDFDKTCQRILGPVSSHTN 687
             +  EC K EQWL E++Q QE L KN DP  WS EI+ +E + D   + I+    S   
Sbjct: 661 EAVDSECTKAEQWLRERSQLQESLPKNVDPAFWSHEIKKKEHELDMFYRNIVRHKGSPAR 720

BLAST of Cucsa.053520.3 vs. TAIR10
Match: AT1G11660.1 (AT1G11660.1 heat shock protein 70 (Hsp 70) family protein)

HSP 1 Score: 573.2 bits (1476), Expect = 2.2e-163
Identity = 274/392 (69.90%), Postives = 338/392 (86.22%), Query Frame = 1

Query: 1   MSVVGFDIGNENCVIAVSRQRGIDVLLNEESQRETPAVIYFGEKQRFLGSAGAASATMNP 60
           MSVVGFD+GNENCVIAV++QRGIDVLLN+ES RE PA++ FGEKQRF+G+A AASATM+P
Sbjct: 1   MSVVGFDVGNENCVIAVAKQRGIDVLLNDESNRENPAMVSFGEKQRFMGAAAAASATMHP 60

Query: 61  RSTISQVKRLIGRNFSEPDVQIELKMFPFKTSEALDGSILVHVKYLGETHTFTPVQIMGM 120
           +STISQ+KRLIGR F EPDVQ +L++FPF+TSE  DG I + ++Y+GE  +F+PVQI+GM
Sbjct: 61  KSTISQLKRLIGRKFREPDVQNDLRLFPFETSEDSDGGIQIRLRYMGEIQSFSPVQILGM 120

Query: 121 LLAHLRDVAEKNLGAPFSDCVIGIPSYFTDLQRRLYWSAAVIAGLKPLRLMHDCTATALS 180
           LL+HL+ +AEK+L  P SDCVIGIPSYFT+ QR  Y  AA IAGL+PLRLMHD TATAL 
Sbjct: 121 LLSHLKQIAEKSLKTPVSDCVIGIPSYFTNSQRLAYLDAAAIAGLRPLRLMHDSTATALG 180

Query: 181 YGIYKTDF-SNTGPIYVAFVDIGHCDTQVSIVSFEPGHMRIMSQTYDRDLGGRDFDEVLF 240
           YGIYKTD  +N+ P Y+ F+DIGHCDTQV + SFE G MR+ S  +DR+LGGRDFDEVLF
Sbjct: 181 YGIYKTDLVANSSPTYIVFIDIGHCDTQVCVASFESGSMRVRSHAFDRNLGGRDFDEVLF 240

Query: 241 SHFVAEFKKNYDIDVNSNVKASIRLRAACEKLKKVLSANLEAVLNIECLMDEKDVKGFIK 300
           +HF  EFK+ Y+IDV +N KA +RLRA+CEK+KKVLSAN EA LNIECLM+EKDV+ FIK
Sbjct: 241 NHFALEFKEKYNIDVYTNTKACVRLRASCEKVKKVLSANAEAQLNIECLMEEKDVRSFIK 300

Query: 301 REEFEKLASGLLEKISIPCTRGLADAGLAVENIHSVELVGSGSRIPAISRLLTSVFKKEP 360
           REEFE+L++GLLE++ +PC + LAD+GL+++ IHSVELVGSGSRIPAIS++L+S+FK+E 
Sbjct: 301 REEFEQLSAGLLERLIVPCQKALADSGLSLDQIHSVELVGSGSRIPAISKMLSSLFKREL 360

Query: 361 SRKLNASECVARGCALQCAMLSPVFRVREYEI 392
            R +NASECVARGCALQCAMLSPVFRVR+YE+
Sbjct: 361 GRTVNASECVARGCALQCAMLSPVFRVRDYEV 392

HSP 2 Score: 249.2 bits (635), Expect = 7.3e-66
Identity = 137/304 (45.07%), Postives = 198/304 (65.13%), Query Frame = 1

Query: 385 RVREYEIGPFQGSNNSNSRVKVRVQLNMNGIITVESATLVE---DTIDQQMPRRDATYSN 444
           ++  + IGPF  S+   +RVKVRVQLN++GI+T++SATL+E   + I  +    +  + +
Sbjct: 457 QISSFMIGPFHISHGEAARVKVRVQLNLHGIVTIDSATLIEYHKENITSEEMISEENHQS 516

Query: 445 TEKMETEFVDSSHSESDARKARGTRRIDIPVSEHIYGGMTKAELLEAQGRELQLAQQDKN 504
           +   +     SS S  +  KA   +R++IPV  ++ G +TK EL EA+ RE  L +QD  
Sbjct: 517 SAMKDGSLDPSSGSIGNEPKA--IKRMEIPVVANVSGALTKDELSEAKQRENSLVEQDLK 576

Query: 505 MEQAKNKKNALESYVYEMRNKLFNTYRSFASDQEREGISSSLQQTEEWLYEDGDDETESA 564
           ME  K+KKNALES+VYEMR+K+ NTYR+ A++ ERE I+ +LQ+TEEWLYEDGDDE+E+A
Sbjct: 577 MESTKDKKNALESFVYEMRDKMLNTYRNTATESERECIARNLQETEEWLYEDGDDESENA 636

Query: 565 YSSKLDGLKKLVDPIINRYEDEEARAQAKAHLLKRISDYRNSGDSLSPQVRALIFEECDK 624
           Y  KL+ +KKL+DPI NR++D E R QA   LLK I+D R + +SL P  +  + +EC K
Sbjct: 637 YIEKLNDVKKLIDPIENRFKDGEERVQASKDLLKTIADNRMAAESLPPPRKNAVLDECHK 696

Query: 625 VEQWLTEKNQQQELLAKNTDPLLWSSEIRTQEEDFDKTCQRILGPVSSHTNSGDSKETNH 684
            E+WL EK  +QE L K+ +P L S+EIR + +  + TC+ I       +NS  +K    
Sbjct: 697 AERWLHEKTTEQESLPKDANPELQSAEIRRKADALNATCKYI-----GKSNSPPAKP--E 751

Query: 685 HNSS 686
           HN S
Sbjct: 757 HNGS 751

BLAST of Cucsa.053520.3 vs. TAIR10
Match: AT1G79930.1 (AT1G79930.1 heat shock protein 91)

HSP 1 Score: 517.7 bits (1332), Expect = 1.1e-146
Identity = 268/519 (51.64%), Postives = 360/519 (69.36%), Query Frame = 1

Query: 1   MSVVGFDIGNENCVIAVSRQRGIDVLLNEESQRETPAVIYFGEKQRFLGSAGAASATMNP 60
           MSVVGFD GNENC++AV+RQRGIDV+LN+ES RETPA++ FG+KQRF+G+AGAAS  MNP
Sbjct: 1   MSVVGFDFGNENCLVAVARQRGIDVVLNDESNRETPAIVCFGDKQRFIGTAGAASTMMNP 60

Query: 61  RSTISQVKRLIGRNFSEPDVQIELKMFPFKTSEALDGSILVHVKYLGETHTFTPVQIMGM 120
           +++ISQ+KRLIGR FS+P++Q ++K  PF  +E  DG  L+H  YLGE   FTP Q+MGM
Sbjct: 61  KNSISQIKRLIGRQFSDPELQRDIKSLPFSVTEGPDGYPLIHANYLGEKRAFTPTQVMGM 120

Query: 121 LLAHLRDVAEKNLGAPFSDCVIGIPSYFTDLQRRLYWSAAVIAGLKPLRLMHDCTATALS 180
           +L++L+ +AEKNL     DC IGIP YFTDLQRR    AA IAGL PLRL+H+ TATAL+
Sbjct: 121 MLSNLKGIAEKNLNTAVVDCCIGIPVYFTDLQRRAVLDAATIAGLHPLRLIHETTATALA 180

Query: 181 YGIYKTDFSNTGPIYVAFVDIGHCDTQVSIVSFEPGHMRIMSQTYDRDLGGRDFDEVLFS 240
           YGIYKTD   +  + VAF+DIGH   QV I  F+ G ++I+S  +DR LGGRDFDEVLF+
Sbjct: 181 YGIYKTDLPESDQLNVAFIDIGHASMQVCIAGFKKGQLKILSHAFDRSLGGRDFDEVLFN 240

Query: 241 HFVAEFKKNYDIDVNSNVKASIRLRAACEKLKKVLSANLEAVLNIECLMDEKDVKGFIKR 300
           HF A+FK  Y IDV+ N KAS+RLRA CEKLKKVLSAN  A LNIECLMDEKDV+G IKR
Sbjct: 241 HFAAKFKDEYKIDVSQNAKASLRLRATCEKLKKVLSANPLAPLNIECLMDEKDVRGVIKR 300

Query: 301 EEFEKLASGLLEKISIPCTRGLADAGLAVENIHSVELVGSGSRIPAISRLLTSVFKKEPS 360
           EEFE+++  +LE++  P  + L+DAGL VE++H VE++GSGSR+PA+ ++LT  F KEP 
Sbjct: 301 EEFEEISIPILERVKRPLEKALSDAGLTVEDVHMVEVIGSGSRVPAMIKILTEFFGKEPR 360

Query: 361 RKLNASECVARGCALQCAMLSPVFRVREYEIG---PFQ---------------GSNNSNS 420
           R +NASECV+RGCALQCA+LSP F+VRE+++    PF                G+ N  S
Sbjct: 361 RTMNASECVSRGCALQCAILSPTFKVREFQVHESFPFSISLAWKGAASEAQNGGAENQQS 420

Query: 421 RVKVRVQLNMNGIITVESATLVEDTIDQQMPRRDATYSNTEKMETEFVDSSHSESDARKA 480
            +   V    N I +V++ T             D  YS+   ++     S+++    + +
Sbjct: 421 TI---VFPKGNPIPSVKALTFYRSGTFSV----DVQYSDVNDLQAPPKISTYTIGPFQSS 480

Query: 481 RGTR-RIDIPVSEHIYG--GMTKAELLEAQGRELQLAQQ 499
           +G R ++ + V  +++G   +  A LLE +  E+ + ++
Sbjct: 481 KGERAKLKVKVRLNLHGIVSVESATLLEEEEVEVPVTKE 512

HSP 2 Score: 166.0 bits (419), Expect = 8.2e-41
Identity = 87/228 (38.16%), Postives = 140/228 (61.40%), Query Frame = 1

Query: 444 KMETEFVDSSHSESDARKARGTRRIDIPVSEHIYGGMTKAELLEAQGRELQLAQQDKNME 503
           +MET+    S +E+  +K + T   ++P+SE +YG +   E+ +A  +E ++A QD+ ME
Sbjct: 565 QMETD----SKAEAPKKKVKKT---NVPLSELVYGALKTVEVEKAVEKEFEMALQDRVME 624

Query: 504 QAKNKKNALESYVYEMRNKLFNTYRSFASDQEREGISSSLQQTEEWLYEDGDDETESAYS 563
           + K++KNA+ESYVY+MRNKL + Y+ + +D ERE   ++LQ+ E+WLYEDG+DET+  Y 
Sbjct: 625 ETKDRKNAVESYVYDMRNKLSDKYQEYITDSEREAFLANLQEVEDWLYEDGEDETKGVYV 684

Query: 564 SKLDGLKKLVDPIINRYEDEEARAQAKAHLLKRISDYRNSGDSLSPQV-------RALIF 623
           +KL+ LKK+ DP+  RY++   R      L   I+ YR +  S  P+        +  + 
Sbjct: 685 AKLEELKKVGDPVEVRYKESLERGSVIDQLGYCINSYREAAMSTDPKFDHIELAEKQKVL 744

Query: 624 EECDKVEQWLTEKNQQQELLAKNTDPLLWSSEIRTQEEDFDKTCQRIL 665
            EC + E WL  K QQQ+ L K   P L S++++++ E  DK C+ I+
Sbjct: 745 NECVEAEAWLRGKQQQQDTLPKYATPALLSADVKSKAEALDKFCRPIM 785

HSP 3 Score: 58.5 bits (140), Expect = 1.8e-08
Identity = 60/234 (25.64%), Postives = 107/234 (45.73%), Query Frame = 1

Query: 385 RVREYEIGPFQGSNNSNSRVKVRVQLNMNGIITVESATLVEDTIDQQMPRRDATYSNTEK 444
           ++  Y IGPFQ S    +++KV+V+LN++GI++VESATL+E+  + ++P    T  ++E 
Sbjct: 461 KISTYTIGPFQSSKGERAKLKVKVRLNLHGIVSVESATLLEEE-EVEVP---VTKEHSE- 520

Query: 445 METEFVDSSHSESDARKARGTRRIDIPVSEHIYGGM-TKAELLEAQGRELQLAQQDKNME 504
            ET  +DS  + ++A  A G   +++  ++       T   + E+  + +Q+    K   
Sbjct: 521 -ETTKMDSDKASAEAAPASGDCDVNMQDAKDTSDATGTDNGVPESAEKPVQMETDSKAEA 580

Query: 505 QAKNKKNA---LESYVY------EMRNKLFNTYRSFASDQEREGISSSLQQTEEWLYE-- 564
             K  K     L   VY      E+   +   +     D+  E         E ++Y+  
Sbjct: 581 PKKKVKKTNVPLSELVYGALKTVEVEKAVEKEFEMALQDRVMEETKDRKNAVESYVYDMR 640

Query: 565 ----DGDDE--TESAYSSKLDGLKKLVDPIINRYEDEEARAQ-AKAHLLKRISD 600
               D   E  T+S   + L  L+++ D +    EDE      AK   LK++ D
Sbjct: 641 NKLSDKYQEYITDSEREAFLANLQEVEDWLYEDGEDETKGVYVAKLEELKKVGD 688

BLAST of Cucsa.053520.3 vs. TAIR10
Match: AT1G79920.1 (AT1G79920.1 Heat shock protein 70 (Hsp 70) family protein)

HSP 1 Score: 515.4 bits (1326), Expect = 5.5e-146
Identity = 273/530 (51.51%), Postives = 365/530 (68.87%), Query Frame = 1

Query: 1   MSVVGFDIGNENCVIAVSRQRGIDVLLNEESQRETPAVIYFGEKQRFLGSAGAASATMNP 60
           MSVVGFD GNENC++AV+RQRGIDV+LN+ES RETPA++ FG+KQRF+G+AGAAS  MNP
Sbjct: 1   MSVVGFDFGNENCLVAVARQRGIDVVLNDESNRETPAIVCFGDKQRFIGTAGAASTMMNP 60

Query: 61  RSTISQVKRLIGRNFSEPDVQIELKMFPFKTSEALDGSILVHVKYLGETHTFTPVQIMGM 120
           +++ISQ+KRLIGR FS+P++Q ++K  PF  +E  DG  L+H  YLGE   FTP Q+MGM
Sbjct: 61  KNSISQIKRLIGRQFSDPELQRDIKSLPFSVTEGPDGYPLIHANYLGEIRAFTPTQVMGM 120

Query: 121 LLAHLRDVAEKNLGAPFSDCVIGIPSYFTDLQRRLYWSAAVIAGLKPLRLMHDCTATALS 180
           +L++L+ +AEKNL     DC IGIP YFTDLQRR    AA IAGL PL L+H+ TATAL+
Sbjct: 121 MLSNLKGIAEKNLNTAVVDCCIGIPVYFTDLQRRAVLDAATIAGLHPLHLIHETTATALA 180

Query: 181 YGIYKTDFSNTGPIYVAFVDIGHCDTQVSIVSFEPGHMRIMSQTYDRDLGGRDFDEVLFS 240
           YGIYKTD      + VAF+DIGH   QV I  F+ G ++I+S  +DR LGGRDFDEVLF+
Sbjct: 181 YGIYKTDLPENDQLNVAFIDIGHASMQVCIAGFKKGQLKILSHAFDRSLGGRDFDEVLFN 240

Query: 241 HFVAEFKKNYDIDVNSNVKASIRLRAACEKLKKVLSANLEAVLNIECLMDEKDVKGFIKR 300
           HF A+FK  Y IDV+ N KAS+RLRA CEKLKKVLSAN  A LNIECLM EKDV+G IKR
Sbjct: 241 HFAAKFKDEYKIDVSQNAKASLRLRATCEKLKKVLSANPMAPLNIECLMAEKDVRGVIKR 300

Query: 301 EEFEKLASGLLEKISIPCTRGLADAGLAVENIHSVELVGSGSRIPAISRLLTSVFKKEPS 360
           EEFE+++  +LE++  P  + L+DAGL VE++H VE+VGSGSR+PA+ ++LT  F KEP 
Sbjct: 301 EEFEEISIPILERVKRPLEKALSDAGLTVEDVHMVEVVGSGSRVPAMIKILTEFFGKEPR 360

Query: 361 RKLNASECVARGCALQCAMLSPVFRVREYEIG---PFQ---------------GSNNSNS 420
           R +NASECV+RGCALQCA+LSP F+VRE+++    PF                G+ N  S
Sbjct: 361 RTMNASECVSRGCALQCAILSPTFKVREFQVHESFPFSISLAWKGAATDAQNGGTENQQS 420

Query: 421 RVKVRVQLNMNGIITVESATLVED---TIDQQMPRRDATYSNTEKMETEFVDSSHSESDA 480
            +   V    N I +V++ T       +ID Q       YS+   ++     S+++    
Sbjct: 421 TI---VFPKGNPIPSVKALTFYRSGTFSIDVQ-------YSDVNDLQAPPKISTYTIGPF 480

Query: 481 RKARGTR-RIDIPVSEHIYG--GMTKAELLEAQGRELQLAQQDKNMEQAK 507
           + ++G R ++ + V  +++G   +  A LLE +  E+ +  +D++ E AK
Sbjct: 481 QSSKGERAKLKVKVRLNLHGIVSVESATLLEEEEVEVSVT-KDQSEETAK 519

HSP 2 Score: 167.9 bits (424), Expect = 2.1e-41
Identity = 88/228 (38.60%), Postives = 141/228 (61.84%), Query Frame = 1

Query: 444 KMETEFVDSSHSESDARKARGTRRIDIPVSEHIYGGMTKAELLEAQGRELQLAQQDKNME 503
           +MET+    S +E+  +K + T   ++P+SE +YG +   E+ +A  +E ++A QD+ ME
Sbjct: 565 QMETD----SKAEAPKKKVKKT---NVPLSELVYGALKTVEVEKAVEKEFEMALQDRVME 624

Query: 504 QAKNKKNALESYVYEMRNKLFNTYRSFASDQEREGISSSLQQTEEWLYEDGDDETESAYS 563
           + K++KNA+ESYVY+MRNKL + Y+ + +D ERE   ++LQ+ E+WLYEDG+DET+  Y 
Sbjct: 625 ETKDRKNAVESYVYDMRNKLSDKYQEYITDSEREAFLANLQEVEDWLYEDGEDETKGVYV 684

Query: 564 SKLDGLKKLVDPIINRYEDEEARAQAKAHLLKRISDYRNSGDSLSPQV-------RALIF 623
           +KL+ LKK+ DP+  RY++   R      L   I+ YR +  S  P+        +  + 
Sbjct: 685 AKLEELKKVGDPVEVRYKESLERGSVIDQLGYCINSYREAAVSNDPKFDHIELAEKQKVL 744

Query: 624 EECDKVEQWLTEKNQQQELLAKNTDPLLWSSEIRTQEEDFDKTCQRIL 665
            EC + E WL EK QQQ+ L K   P L S++++++ E  DK C+ I+
Sbjct: 745 NECVEAEAWLREKQQQQDTLPKYATPALLSADVKSKAEALDKFCRPIM 785

HSP 3 Score: 58.9 bits (141), Expect = 1.4e-08
Identity = 77/333 (23.12%), Postives = 139/333 (41.74%), Query Frame = 1

Query: 385 RVREYEIGPFQGSNNSNSRVKVRVQLNMNGIITVESATLVEDTIDQQMPRRDATYSNTEK 444
           ++  Y IGPFQ S    +++KV+V+LN++GI++VESATL+E+         + + +  + 
Sbjct: 461 KISTYTIGPFQSSKGERAKLKVKVRLNLHGIVSVESATLLEE------EEVEVSVTKDQS 520

Query: 445 METEFVDSSHSESDARKARGTRRIDIPVSEHIYGGM-TKAELLEAQGRELQLAQQDKNME 504
            ET  +D+  + ++A  A G   +++  ++       T   + E+  + +Q+    K   
Sbjct: 521 EETAKMDTDKASAEAAPASGDSDVNMQDAKDTSDATGTDNGVPESAEKPVQMETDSKAEA 580

Query: 505 QAKNKKNA---LESYVY------EMRNKLFNTYRSFASDQEREGISSSLQQTEEWLYE-- 564
             K  K     L   VY      E+   +   +     D+  E         E ++Y+  
Sbjct: 581 PKKKVKKTNVPLSELVYGALKTVEVEKAVEKEFEMALQDRVMEETKDRKNAVESYVYDMR 640

Query: 565 ----DGDDE--TESAYSSKLDGLKKLVDPIINRYEDEEARAQ-AKAHLLKRISD-----Y 624
               D   E  T+S   + L  L+++ D +    EDE      AK   LK++ D     Y
Sbjct: 641 NKLSDKYQEYITDSEREAFLANLQEVEDWLYEDGEDETKGVYVAKLEELKKVGDPVEVRY 700

Query: 625 RNS----------GDSLSPQVRALIFEE--CDKVEQWLTEK----NQQQEL--------- 665
           + S          G  ++    A +  +   D +E  L EK    N+  E          
Sbjct: 701 KESLERGSVIDQLGYCINSYREAAVSNDPKFDHIE--LAEKQKVLNECVEAEAWLREKQQ 760

BLAST of Cucsa.053520.3 vs. TAIR10
Match: AT5G42020.1 (AT5G42020.1 Heat shock protein 70 (Hsp 70) family protein)

HSP 1 Score: 260.0 bits (663), Expect = 4.2e-69
Identity = 175/611 (28.64%), Postives = 298/611 (48.77%), Query Frame = 1

Query: 2   SVVGFDIGNENCVIAVSRQRGIDVLLNEESQRETPAVIYFGEKQRFLGSAGAASATMNPR 61
           SV+G D+G     + V +   ++++ N++  R TP+ + F + +R +G A    A +NP 
Sbjct: 36  SVIGIDLGTTYSCVGVYKNGHVEIIANDQGNRITPSWVGFTDSERLIGEAAKNQAAVNPE 95

Query: 62  STISQVKRLIGRNFSEPDVQIELKMFPFKTSEALDGSILVHVKYL-GETHTFTPVQIMGM 121
            T+  VKRLIGR F + +VQ + K+ P++     DG   + VK   GET  F+P +I  M
Sbjct: 96  RTVFDVKRLIGRKFEDKEVQKDRKLVPYQIVNK-DGKPYIQVKIKDGETKVFSPEEISAM 155

Query: 122 LLAHLRDVAEKNLGAPFSDCVIGIPSYFTDLQRRLYWSAAVIAGLKPLRLMHDCTATALS 181
           +L  +++ AE  LG    D V+ +P+YF D QR+    A VIAGL   R++++ TA A++
Sbjct: 156 ILTKMKETAEAYLGKKIKDAVVTVPAYFNDAQRQATKDAGVIAGLNVARIINEPTAAAIA 215

Query: 182 YGIYKTDFSNTGPIYVAFVDIGHCDTQVSIVSFEPGHMRIMSQTYDRDLGGRDFDEVLFS 241
           YG+ K      G   +   D+G     VS+++ + G   ++S   D  LGG DFD  +  
Sbjct: 216 YGLDKKG----GEKNILVFDLGGGTFDVSVLTIDNGVFEVLSTNGDTHLGGEDFDHRIME 275

Query: 242 HFVAEFKKNYDIDVNSNVKASIRLRAACEKLKKVLSANLEAVLNIECLMDEKDVKGFIKR 301
           +F+   KK +  D++ + KA  +LR  CE+ K+ LS+  +  + IE L D  D+   + R
Sbjct: 276 YFIKLIKKKHQKDISKDNKALGKLRRECERAKRALSSQHQVRVEIESLFDGVDLSEPLTR 335

Query: 302 EEFEKLASGLLEKISIPCTRGLADAGLAVENIHSVELVGSGSRIPAISRLLTSVFK-KEP 361
             FE+L + L  K   P  + + DAGL    I  + LVG  +RIP + +LL   F+ KEP
Sbjct: 336 ARFEELNNDLFRKTMGPVKKAMDDAGLQKSQIDEIVLVGGSTRIPKVQQLLKDFFEGKEP 395

Query: 362 SRKLNASECVARGCALQCAMLS-----PVFRVREYEIGPFQGSNNSNSRVKVRVQLNMNG 421
           ++ +N  E VA G A+Q  +LS         +   ++ P      +   V  ++ +  N 
Sbjct: 396 NKGVNPDEAVAYGAAVQGGILSGEGGDETKDILLLDVAPLTLGIETVGGVMTKL-IPRNT 455

Query: 422 IITVESATLVEDTIDQQMPRRDATYSNTEKM--ETEFVDSSHSESDARKARGTRRID--- 481
           +I  + + +     DQQ       +     +  +   +            RGT +I+   
Sbjct: 456 VIPTKKSQVFTTYQDQQTTVSIQVFEGERSLTKDCRLLGKFDLTGVPPAPRGTPQIEVTF 515

Query: 482 ---------IPVSEHIYGGMTKAELLEAQGRELQ------------LAQQDKNMEQAKNK 541
                    +   +   G   K  +   +GR  Q             A++DK +++  + 
Sbjct: 516 EVDANGILNVKAEDKASGKSEKITITNEKGRLSQEEIDRMVKEAEEFAEEDKKVKEKIDA 575

Query: 542 KNALESYVYEMRNKLFNTYR--SFASDQEREGISSSLQQTEEWLYEDGDDETESAYSSKL 578
           +NALE+YVY M+N++ +  +        E+E I ++ ++  EWL E+ + E E  Y  KL
Sbjct: 576 RNALETYVYNMKNQVSDKDKLADKLEGDEKEKIEAATKEALEWLDENQNSEKEE-YDEKL 635

BLAST of Cucsa.053520.3 vs. TAIR10
Match: AT5G28540.1 (AT5G28540.1 heat shock protein 70 (Hsp 70) family protein)

HSP 1 Score: 258.8 bits (660), Expect = 9.3e-69
Identity = 175/611 (28.64%), Postives = 297/611 (48.61%), Query Frame = 1

Query: 2   SVVGFDIGNENCVIAVSRQRGIDVLLNEESQRETPAVIYFGEKQRFLGSAGAASATMNPR 61
           SV+G D+G     + V +   ++++ N++  R TP+ + F + +R +G A    A +NP 
Sbjct: 36  SVIGIDLGTTYSCVGVYKNGHVEIIANDQGNRITPSWVGFTDSERLIGEAAKNQAAVNPE 95

Query: 62  STISQVKRLIGRNFSEPDVQIELKMFPFKTSEALDGSILVHVKYL-GETHTFTPVQIMGM 121
            T+  VKRLIGR F + +VQ + K+ P++     DG   + VK   GET  F+P +I  M
Sbjct: 96  RTVFDVKRLIGRKFEDKEVQKDRKLVPYQIVNK-DGKPYIQVKIKDGETKVFSPEEISAM 155

Query: 122 LLAHLRDVAEKNLGAPFSDCVIGIPSYFTDLQRRLYWSAAVIAGLKPLRLMHDCTATALS 181
           +L  +++ AE  LG    D V+ +P+YF D QR+    A VIAGL   R++++ TA A++
Sbjct: 156 ILTKMKETAEAYLGKKIKDAVVTVPAYFNDAQRQATKDAGVIAGLNVARIINEPTAAAIA 215

Query: 182 YGIYKTDFSNTGPIYVAFVDIGHCDTQVSIVSFEPGHMRIMSQTYDRDLGGRDFDEVLFS 241
           YG+ K      G   +   D+G     VS+++ + G   ++S   D  LGG DFD  +  
Sbjct: 216 YGLDKKG----GEKNILVFDLGGGTFDVSVLTIDNGVFEVLSTNGDTHLGGEDFDHRVME 275

Query: 242 HFVAEFKKNYDIDVNSNVKASIRLRAACEKLKKVLSANLEAVLNIECLMDEKDVKGFIKR 301
           +F+   KK +  D++ + KA  +LR  CE+ K+ LS+  +  + IE L D  D    + R
Sbjct: 276 YFIKLIKKKHQKDISKDNKALGKLRRECERAKRALSSQHQVRVEIESLFDGVDFSEPLTR 335

Query: 302 EEFEKLASGLLEKISIPCTRGLADAGLAVENIHSVELVGSGSRIPAISRLLTSVFK-KEP 361
             FE+L + L  K   P  + + DAGL    I  + LVG  +RIP + +LL   F+ KEP
Sbjct: 336 ARFEELNNDLFRKTMGPVKKAMDDAGLQKSQIDEIVLVGGSTRIPKVQQLLKDFFEGKEP 395

Query: 362 SRKLNASECVARGCALQCAMLS-----PVFRVREYEIGPFQGSNNSNSRVKVRVQLNMNG 421
           ++ +N  E VA G A+Q  +LS         +   ++ P      +   V  ++ +  N 
Sbjct: 396 NKGVNPDEAVAYGAAVQGGILSGEGGDETKDILLLDVAPLTLGIETVGGVMTKL-IPRNT 455

Query: 422 IITVESATLVEDTIDQQMPRRDATYSNTEKM--ETEFVDSSHSESDARKARGTRRID--- 481
           +I  + + +     DQQ       +     +  +   +            RGT +I+   
Sbjct: 456 VIPTKKSQVFTTYQDQQTTVSIQVFEGERSLTKDCRLLGKFDLNGIPPAPRGTPQIEVTF 515

Query: 482 ---------IPVSEHIYGGMTKAELLEAQGRELQ------------LAQQDKNMEQAKNK 541
                    +   +   G   K  +   +GR  Q             A++DK +++  + 
Sbjct: 516 EVDANGILNVKAEDKASGKSEKITITNEKGRLSQEEIDRMVKEAEEFAEEDKKVKEKIDA 575

Query: 542 KNALESYVYEMRNKLFNTYR--SFASDQEREGISSSLQQTEEWLYEDGDDETESAYSSKL 578
           +NALE+YVY M+N++ +  +        E+E I ++ ++  EWL E+ + E E  Y  KL
Sbjct: 576 RNALETYVYNMKNQVNDKDKLADKLEGDEKEKIEAATKEALEWLDENQNSEKEE-YDEKL 635

BLAST of Cucsa.053520.3 vs. NCBI nr
Match: gi|763754738|gb|KJB22069.1| (hypothetical protein B456_004G027900 [Gossypium raimondii])

HSP 1 Score: 918.3 bits (2372), Expect = 8.0e-264
Identity = 462/676 (68.34%), Postives = 540/676 (79.88%), Query Frame = 1

Query: 1   MSVVGFDIGNENCVIAVSRQRGIDVLLNEESQRETPAVIYFGEKQRFLGSAGAASATMNP 60
           MSVVGFDIGNE CVIA  +QRG+DVLLN+ES+RETPA++ FGEKQRFLGSA AASA M+P
Sbjct: 1   MSVVGFDIGNEKCVIAAVKQRGVDVLLNDESKRETPALVCFGEKQRFLGSAAAASAMMHP 60

Query: 61  RSTISQVKRLIGRNFSEPDVQIELKMFPFKTSEALDGSILVHVKYLGETHTFTPVQIMGM 120
           ++T+SQVKRLIGR F +PDVQ EL+M PF+TSE  DG IL+H+KYLGETH FTPVQIM M
Sbjct: 61  KTTVSQVKRLIGRKFQDPDVQNELRMLPFETSEGQDGGILIHLKYLGETHRFTPVQIMAM 120

Query: 121 LLAHLRDVAEKNLGAPFSDCVIGIPSYFTDLQRRLYWSAAVIAGLKPLRLMHDCTATALS 180
           L AHL+ + E NLG P  DCVIGIPSYFTDLQRR Y  AA IAGLKPLRLMHDCTATAL 
Sbjct: 121 LFAHLKYMTETNLGLPVLDCVIGIPSYFTDLQRRAYLDAAKIAGLKPLRLMHDCTATALG 180

Query: 181 YGIYKTDFSNTGPIYVAFVDIGHCDTQVSIVSFEPGHMRIMSQTYDRDLGGRDFDEVLFS 240
           YGIYKTDFSN GP  VAFVDIGHCDTQVSIVSFE G MRI+S  +D  LGGRDFDE+LF 
Sbjct: 181 YGIYKTDFSNAGPTNVAFVDIGHCDTQVSIVSFEAGQMRILSHAFDNSLGGRDFDEILFG 240

Query: 241 HFVAEFKKNYDIDVNSNVKASIRLRAACEKLKKVLSANLEAVLNIECLMDEKDVKGFIKR 300
           +F A FK+ Y+IDV SNV+ASIRLRAACEKLKKVLSAN EA LNIECLMDEKDVKGFIKR
Sbjct: 241 YFAACFKEQYNIDVYSNVRASIRLRAACEKLKKVLSANAEAPLNIECLMDEKDVKGFIKR 300

Query: 301 EEFEKLASGLLEKISIPCTRGLADAGLAVENIHSVELVGSGSRIPAISRLLTSVFKKEPS 360
           EEFEKLASGLLE+I+IPCT+ + DAGL V  I +VELVGSGSRIPAI+R L S F++EPS
Sbjct: 301 EEFEKLASGLLERINIPCTKAITDAGLTVGKILAVELVGSGSRIPAITRQLASFFRREPS 360

Query: 361 RKLNASECVARGCALQCAMLSPVFRVREYEIGPFQGSNNSNSRVKVRVQLNMNGIITVES 420
           R +NASECVARGCALQCAMLSP+FRVR+YEIGPFQ SN+  +RVKV+VQLN++GI+ VES
Sbjct: 361 RTINASECVARGCALQCAMLSPIFRVRDYEIGPFQASNSERARVKVKVQLNLHGIVNVES 420

Query: 421 ATLVEDTI-DQQMPRRDATYSNTEKMETEFVDSSHSESDA---RKARGTRRIDIPVSEHI 480
           A L+E+ + D ++  ++  +      +  ++    S + A      +  RR++IPVSE+I
Sbjct: 421 AMLIEEHVDDSELSTKEVRHVTNGSEDGTYMQPKPSNASADGKTNDKTMRRLEIPVSENI 480

Query: 481 YGGMTKAELLEAQGRELQLAQQDKNMEQAKNKKNALESYVYEMRNKLFNTYRSFASDQER 540
            G MT  EL+EAQ +EL+LAQQD+ MEQ K KKNALESYVYEMRNKLFN YR FASD+ER
Sbjct: 481 NGTMTGVELMEAQDKELKLAQQDRTMEQTKEKKNALESYVYEMRNKLFNIYRGFASDEER 540

Query: 541 EGISSSLQQTEEWLYEDGDDETESAYSSKLDGLKKLVDPIINRYEDEEARAQAKAHLLKR 600
           EGIS SLQ+TEEWLY+DG+DETE AY+SKL+ LKKLVDP+ NR++DEEAR QA   L K 
Sbjct: 541 EGISKSLQETEEWLYDDGEDETEGAYTSKLEALKKLVDPVENRFKDEEARTQASTDLFKC 600

Query: 601 ISDYRNSGDSLSPQVRALIFEECDKVEQWLTEKNQQQELLAKNTDPLLWSSEIRTQEEDF 660
           I+D R S  SL  + R  I  EC+K EQWLTEK +QQ  L KN DPLLWSSEI+   ED 
Sbjct: 601 IADIRMSTKSLPNEDRESILNECNKAEQWLTEKTKQQHSLPKNIDPLLWSSEIKKMTEDL 660

Query: 661 DKTCQRILGPVSSHTN 673
           +  C  I+   +SH N
Sbjct: 661 NMKCMHIMSRKASHRN 676

BLAST of Cucsa.053520.3 vs. NCBI nr
Match: gi|449468540|ref|XP_004151979.1| (PREDICTED: heat shock 70 kDa protein 16 [Cucumis sativus])

HSP 1 Score: 771.2 bits (1990), Expect = 1.6e-219
Identity = 389/391 (99.49%), Postives = 391/391 (100.00%), Query Frame = 1

Query: 1   MSVVGFDIGNENCVIAVSRQRGIDVLLNEESQRETPAVIYFGEKQRFLGSAGAASATMNP 60
           MSVVGFDIGNENCVIAVSRQRGIDVLLNEESQRETPAVIYFGEKQRFLGSAGAASATMNP
Sbjct: 1   MSVVGFDIGNENCVIAVSRQRGIDVLLNEESQRETPAVIYFGEKQRFLGSAGAASATMNP 60

Query: 61  RSTISQVKRLIGRNFSEPDVQIELKMFPFKTSEALDGSILVHVKYLGETHTFTPVQIMGM 120
           RSTISQVKRLIGRNFSEPDVQIELKMFPFKTSEALDGSILVHVKYLGETHTFTPVQIMGM
Sbjct: 61  RSTISQVKRLIGRNFSEPDVQIELKMFPFKTSEALDGSILVHVKYLGETHTFTPVQIMGM 120

Query: 121 LLAHLRDVAEKNLGAPFSDCVIGIPSYFTDLQRRLYWSAAVIAGLKPLRLMHDCTATALS 180
           LLAHL+DVAEKNLGAPFSDCVIGIPSYFTDLQRRLYWSAAVIAGLKPLRLMHDCTATALS
Sbjct: 121 LLAHLKDVAEKNLGAPFSDCVIGIPSYFTDLQRRLYWSAAVIAGLKPLRLMHDCTATALS 180

Query: 181 YGIYKTDFSNTGPIYVAFVDIGHCDTQVSIVSFEPGHMRIMSQTYDRDLGGRDFDEVLFS 240
           YGIYKTDFSNTGPIYVAFVDIGHCDTQVSIVSFEPGHMRIMSQTYDRDLGGRDFDEVLFS
Sbjct: 181 YGIYKTDFSNTGPIYVAFVDIGHCDTQVSIVSFEPGHMRIMSQTYDRDLGGRDFDEVLFS 240

Query: 241 HFVAEFKKNYDIDVNSNVKASIRLRAACEKLKKVLSANLEAVLNIECLMDEKDVKGFIKR 300
           HFVAEFKKNYDIDVNSNVKASIRLRAACEKLKKVLSANLEAVLNIECLMDEKDVKGFIKR
Sbjct: 241 HFVAEFKKNYDIDVNSNVKASIRLRAACEKLKKVLSANLEAVLNIECLMDEKDVKGFIKR 300

Query: 301 EEFEKLASGLLEKISIPCTRGLADAGLAVENIHSVELVGSGSRIPAISRLLTSVFKKEPS 360
           EEFEKLASGLLEKISIPCTRGLADAGLAVENIHSVELVGSGSRIPAISRLLTSVFKKEPS
Sbjct: 301 EEFEKLASGLLEKISIPCTRGLADAGLAVENIHSVELVGSGSRIPAISRLLTSVFKKEPS 360

Query: 361 RKLNASECVARGCALQCAMLSPVFRVREYEI 392
           RKLNASECVARGCALQCAMLSPVFRVREYE+
Sbjct: 361 RKLNASECVARGCALQCAMLSPVFRVREYEV 391

BLAST of Cucsa.053520.3 vs. NCBI nr
Match: gi|449468540|ref|XP_004151979.1| (PREDICTED: heat shock 70 kDa protein 16 [Cucumis sativus])

HSP 1 Score: 594.3 bits (1531), Expect = 2.6e-166
Identity = 299/303 (98.68%), Postives = 301/303 (99.34%), Query Frame = 1

Query: 389 YEIGPFQGSNNSNSRVKVRVQLNMNGIITVESATLVEDTIDQQMPRRDATYSNTEKMETE 448
           + IGPFQGSNNSNSRVKVRVQLNMNGIITVESATLVEDTIDQQMPRRDATYSNTEKMETE
Sbjct: 460 FTIGPFQGSNNSNSRVKVRVQLNMNGIITVESATLVEDTIDQQMPRRDATYSNTEKMETE 519

Query: 449 FVDSSHSESD-ARKARGTRRIDIPVSEHIYGGMTKAELLEAQGRELQLAQQDKNMEQAKN 508
           FVDSSHSESD +RKARGTRRIDIPVSEHIYGGMTKAELLEAQGRELQLAQQDKNMEQAKN
Sbjct: 520 FVDSSHSESDVSRKARGTRRIDIPVSEHIYGGMTKAELLEAQGRELQLAQQDKNMEQAKN 579

Query: 509 KKNALESYVYEMRNKLFNTYRSFASDQEREGISSSLQQTEEWLYEDGDDETESAYSSKLD 568
           KKNALESYVYEMRNKLFNTYRSFASDQEREGISSSLQQTEEWLYEDGDDETESAYSSKLD
Sbjct: 580 KKNALESYVYEMRNKLFNTYRSFASDQEREGISSSLQQTEEWLYEDGDDETESAYSSKLD 639

Query: 569 GLKKLVDPIINRYEDEEARAQAKAHLLKRISDYRNSGDSLSPQVRALIFEECDKVEQWLT 628
           GLKKLVDPIINRYEDEEARAQAKAHLLKRISDYRNSGDSLSPQVRALIFEECDKVEQWLT
Sbjct: 640 GLKKLVDPIINRYEDEEARAQAKAHLLKRISDYRNSGDSLSPQVRALIFEECDKVEQWLT 699

Query: 629 EKNQQQELLAKNTDPLLWSSEIRTQEEDFDKTCQRILGPVSSHTNSGDSKETNHHNSSDN 688
           EKNQQQELLAKNTDPLLWSSEIRTQEEDFDKTCQRILGPVSSHTNSGDSKETNHHNSSDN
Sbjct: 700 EKNQQQELLAKNTDPLLWSSEIRTQEEDFDKTCQRILGPVSSHTNSGDSKETNHHNSSDN 759

Query: 689 HHS 691
           HHS
Sbjct: 760 HHS 762

HSP 2 Score: 752.3 bits (1941), Expect = 7.6e-214
Identity = 442/717 (61.65%), Postives = 500/717 (69.74%), Query Frame = 1

Query: 1   MSVVGFDIGNENCVIAVSRQRGIDVLLNEESQRETPAVIYFGEKQRFLGSAGAASATMNP 60
           MSVVGFDIGNENCVIAVSRQRGIDVLLNEESQRET AVI FGEKQRFLGSA AASATMNP
Sbjct: 1   MSVVGFDIGNENCVIAVSRQRGIDVLLNEESQRETSAVICFGEKQRFLGSAAAASATMNP 60

Query: 61  RSTISQVKRLIGRNFSEPDVQIELKMFPFKTSEALDGSILVHVKYLGETHTFTPVQIMGM 120
           RSTISQVKRLIGRNF+EP VQI+LKMFPFKTSEALDGSILVHVKYLGETHTFTPVQIMGM
Sbjct: 61  RSTISQVKRLIGRNFTEPGVQIDLKMFPFKTSEALDGSILVHVKYLGETHTFTPVQIMGM 120

Query: 121 LLAHLRDVAEKNLGAPFSDCVIGIPSYFTDLQRRLYWSAAVIAGLKPLRLMHDCTATALS 180
           LLAHLRDVAEKNLGAPFSDCVIGIPSYFTDLQRRLYW+AA IAGLKPLRLMHDCTATALS
Sbjct: 121 LLAHLRDVAEKNLGAPFSDCVIGIPSYFTDLQRRLYWNAAAIAGLKPLRLMHDCTATALS 180

Query: 181 YGIYKTDFSNTGPIYVAFVDIGHCDTQVSIVSFEPGHMRIMSQTYDRDLGGRDFDEVLFS 240
           YGIYKTDFSNTGPIYVAFVDIGHCDTQVSIVSFEPGHMRIMSQTYDRDLGGRDFDEVLFS
Sbjct: 181 YGIYKTDFSNTGPIYVAFVDIGHCDTQVSIVSFEPGHMRIMSQTYDRDLGGRDFDEVLFS 240

Query: 241 HFVAEFKKNYDIDVNSNVKASIRLRAACEKLKKVLSANLEAVLNIECLMDEKDVKGFIKR 300
           HFVAEFKKNYDIDVN NVKASIRLRAACEKLKKVLSANLEAVLNIECLMDEKDVKGFIKR
Sbjct: 241 HFVAEFKKNYDIDVNLNVKASIRLRAACEKLKKVLSANLEAVLNIECLMDEKDVKGFIKR 300

Query: 301 EEFEKLASGLLEKISIPCTRGLADAGLAVENIHSVELVGSGSRIPAISRLLTSVFKKEPS 360
           EEFEKLASGLLEKISIPCTRGLADAGLAVE IHSVELVGSGSRIPAI+RLLTSVFKKEPS
Sbjct: 301 EEFEKLASGLLEKISIPCTRGLADAGLAVEKIHSVELVGSGSRIPAITRLLTSVFKKEPS 360

Query: 361 RKLNASECVARGCALQCAMLSPVFRVREYEIG---PFQGSNNSNSRVKVRVQLNMNGIIT 420
           RKLNASECVARGCALQCAMLSPVFRVREYE+    PF    +S++     + L +N ++ 
Sbjct: 361 RKLNASECVARGCALQCAMLSPVFRVREYEVQDSFPFSIGFSSDAG---PISLGLNNVLF 420

Query: 421 VES----ATLVEDTIDQQMPRRDATYSNTEKMET------------EFVDSSHSESDARK 480
            +     +T +       +   +A YSN +++               F  S++S S    
Sbjct: 421 PKGQHIPSTKILSFQRNSLFHLEAVYSNPDELPPGMSSKIGCFTIGPFQGSNNSNS---- 480

Query: 481 ARGTRRIDIPVSEHIYGGMT--KAELLEAQGRELQLAQQDKNMEQAKNKKNALESYVYEM 540
                R+ + V  ++ G +T   A ++E              ++Q  ++++A  S   +M
Sbjct: 481 -----RVKVRVQLNMNGIITVESATIVE------------DTIDQQISRRDATHSNTEKM 540

Query: 541 RNKLFNTYRSFASDQEREG---------ISSSLQ--QTEEWLYEDGDDETESAYSSKLDG 600
             +  +++ S  SD  R+          +S  +    T+  L E    E + A   K   
Sbjct: 541 ETEFVDSFHS-ESDVSRKARGTRRIDIPVSEHIYGGMTKAELLEAQGRELQLAQQDKNME 600

Query: 601 LKKLVDPIINRYEDEEARAQAKAHLLKRISDYRNSGDSLSPQVRALIFEECDKVEQWLTE 660
             K     +  Y  E           K  + YR+     S Q R  I     + E+WL E
Sbjct: 601 QAKNKKNALESYVYEMRN--------KLFNTYRSFA---SDQEREGISSSLQQTEEWLYE 660

Query: 661 KNQQQ---------ELLAKNTDPLLWSSEIRTQEEDFDKTCQRILGPVSSHTNSGDS 677
               +         + L K  DP++   E    EE   +    +L  +S + NSGDS
Sbjct: 661 DGDDETESAYSTKLDDLKKLVDPIINRYE---DEEARAQAKAHLLKRISDYRNSGDS 678

BLAST of Cucsa.053520.3 vs. NCBI nr
Match: gi|659130265|ref|XP_008465078.1| (PREDICTED: heat shock 70 kDa protein 16 [Cucumis melo])

HSP 1 Score: 568.2 bits (1463), Expect = 2.0e-158
Identity = 286/303 (94.39%), Postives = 296/303 (97.69%), Query Frame = 1

Query: 389 YEIGPFQGSNNSNSRVKVRVQLNMNGIITVESATLVEDTIDQQMPRRDATYSNTEKMETE 448
           + IGPFQGSNNSNSRVKVRVQLNMNGIITVESAT+VEDTIDQQ+ RRDAT+SNTEKMETE
Sbjct: 460 FTIGPFQGSNNSNSRVKVRVQLNMNGIITVESATIVEDTIDQQISRRDATHSNTEKMETE 519

Query: 449 FVDSSHSESD-ARKARGTRRIDIPVSEHIYGGMTKAELLEAQGRELQLAQQDKNMEQAKN 508
           FVDS HSESD +RKARGTRRIDIPVSEHIYGGMTKAELLEAQGRELQLAQQDKNMEQAKN
Sbjct: 520 FVDSFHSESDVSRKARGTRRIDIPVSEHIYGGMTKAELLEAQGRELQLAQQDKNMEQAKN 579

Query: 509 KKNALESYVYEMRNKLFNTYRSFASDQEREGISSSLQQTEEWLYEDGDDETESAYSSKLD 568
           KKNALESYVYEMRNKLFNTYRSFASDQEREGISSSLQQTEEWLYEDGDDETESAYS+KLD
Sbjct: 580 KKNALESYVYEMRNKLFNTYRSFASDQEREGISSSLQQTEEWLYEDGDDETESAYSTKLD 639

Query: 569 GLKKLVDPIINRYEDEEARAQAKAHLLKRISDYRNSGDSLSPQVRALIFEECDKVEQWLT 628
            LKKLVDPIINRYEDEEARAQAKAHLLKRISDYRNSGDSLSPQVRALIFEECD+VEQWLT
Sbjct: 640 DLKKLVDPIINRYEDEEARAQAKAHLLKRISDYRNSGDSLSPQVRALIFEECDRVEQWLT 699

Query: 629 EKNQQQELLAKNTDPLLWSSEIRTQEEDFDKTCQRILGPVSSHTNSGDSKETNHHNSSDN 688
           EKNQQQEL+AK+TDPLLWSSEIRTQEEDFDKTCQRIL PVSS+TNSGDSKETNH NSSDN
Sbjct: 700 EKNQQQELIAKHTDPLLWSSEIRTQEEDFDKTCQRILRPVSSNTNSGDSKETNHQNSSDN 759

Query: 689 HHS 691
           HHS
Sbjct: 760 HHS 762

HSP 2 Score: 656.4 bits (1692), Expect = 5.6e-185
Identity = 355/784 (45.28%), Postives = 496/784 (63.27%), Query Frame = 1

Query: 1   MSVVGFDIGNENCVIAVSRQRGIDVLLNEESQRETPAVIYFGEKQRFLGSAGAASATMNP 60
           MSVVGFD GNE+ V+AV+RQRGIDV+LN+ES+RETPA++ FGEKQRFLG+AGAAS+ MNP
Sbjct: 1   MSVVGFDFGNESGVVAVARQRGIDVVLNDESKRETPAIVCFGEKQRFLGTAGAASSMMNP 60

Query: 61  RSTISQVKRLIGRNFSEPDVQIELKMFPFKTSEALDGSILVHVKYLGETHTFTPVQIMGM 120
           ++TISQ+KRLIGR FS+P++Q ++K  PF  +E  DG  L++ +YLGE  +FTP Q++GM
Sbjct: 61  KNTISQIKRLIGRQFSDPELQRDIKSLPFSVTEGPDGYPLINAQYLGEMRSFTPTQVLGM 120

Query: 121 LLAHLRDVAEKNLGAPFSDCVIGIPSYFTDLQRRLYWSAAVIAGLKPLRLMHDCTATALS 180
           + + L+ +AEKNL A   DC IGIP YFTDLQRR    AA IAGL PLRL+H+ TATAL+
Sbjct: 121 VFSDLKSIAEKNLNAAVVDCCIGIPIYFTDLQRRAVLDAATIAGLHPLRLIHETTATALA 180

Query: 181 YGIYKTDFSNTGPIYVAFVDIGHCDTQVSIVSFEPGHMRIMSQTYDRDLGGRDFDEVLFS 240
           YGIYKTD      + VAFVDIGH   QV I   + G ++I++  +DR LGGRDFDEVLF 
Sbjct: 181 YGIYKTDLPENDQLNVAFVDIGHASMQVCIAGLKKGQLKILAHAFDRSLGGRDFDEVLFQ 240

Query: 241 HFVAEFKKNYDIDVNSNVKASIRLRAACEKLKKVLSANLEAVLNIECLMDEKDVKGFIKR 300
           +F  +FK  Y IDV  N +AS+RLRAACEKLKKVLSAN EA LNIECLMDEKDV+GFIKR
Sbjct: 241 NFAEKFKTEYKIDVYQNARASLRLRAACEKLKKVLSANPEAPLNIECLMDEKDVRGFIKR 300

Query: 301 EEFEKLASGLLEKISIPCTRGLADAGLAVENIHSVELVGSGSRIPAISRLLTSVFKKEPS 360
           +EFE+++  +LE++  P  + L++AGL VENI++VE+VGSGSR+PAI ++LT  F KEP 
Sbjct: 301 DEFEQISIPILERVKKPLEKALSEAGLTVENIYAVEVVGSGSRVPAIMKILTEFFGKEPR 360

Query: 361 RKLNASECVARGCALQ-------------------------------------------- 420
           R +NASECVA+GCAL+                                            
Sbjct: 361 RTMNASECVAKGCALECAILSPTFKVREFQVNESFPFTIALSWKGTAPDAQNGADNQQID 420

Query: 421 --CAMLSPV---FRVREYEIGPFQGSNNSNSRVKVRVQLNMNGIITVESATLVEDTID-- 480
              A ++ V     +  Y IGPFQ + +  ++VKV+V+LN++G++++ESATL+E+ ++  
Sbjct: 421 VHYADVNEVQAPAMISTYTIGPFQSTKSERAKVKVKVRLNLHGVVSIESATLLEEDVEIP 480

Query: 481 --QQMPRRDATYS-----------NTEKMETEFVDSSHSESDARKARG------------ 540
             ++  + DA  S           N   ME     ++++   A  A+G            
Sbjct: 481 VVKETAKMDADESPPVPAPSSGAENDVDMEDAKSTAANAPGSANGAQGDVPVQMETESKV 540

Query: 541 ------TRRIDIPVSEHIYGGMTKAELLEAQGRELQLAQQDKNMEQAKNKKNALESYVYE 600
                  ++ DIPV+E +YGGM   ++ +A  +E ++A QD+ ME+ K+KKNA+E+YVY+
Sbjct: 541 EVPKKKVKKTDIPVTELVYGGMQPTDVQKAVEKEFEMALQDRVMEETKDKKNAVEAYVYD 600

Query: 601 MRNKLFNTYRSFASDQEREGISSSLQQTEEWLYEDGDDETESAYSSKLDGLKKLVDPIIN 660
           MRNKL + Y  F +D ERE  ++ LQQ E+WLYEDG+DET+  Y +KL+ LKK  DP+  
Sbjct: 601 MRNKLHDKYHDFVTDPEREEFTAILQQVEDWLYEDGEDETKGVYVAKLEELKKRGDPVEL 660

Query: 661 RYEDEEARAQAKAHLLKRISDYR-------NSGDSLSPQVRALIFEECDKVEQWLTEKNQ 687
           RY++   R      L+  I+ YR       +  D +    +  + +EC   E WL EK Q
Sbjct: 661 RYKEHLERGSVVDQLVYCINSYREAVMSPDSKFDHIDIAEKEKVLKECADAEAWLREKKQ 720

BLAST of Cucsa.053520.3 vs. NCBI nr
Match: gi|645241092|ref|XP_008226915.1| (PREDICTED: heat shock 70 kDa protein 16 [Prunus mume])

HSP 1 Score: 640.6 bits (1651), Expect = 3.2e-180
Identity = 314/391 (80.31%), Postives = 348/391 (89.00%), Query Frame = 1

Query: 1   MSVVGFDIGNENCVIAVSRQRGIDVLLNEESQRETPAVIYFGEKQRFLGSAGAASATMNP 60
           MSVVGFDIGNENCVIAV +QRG+DVLLN+ES+RETPAV+ FGEKQRFLGSAGAASA MNP
Sbjct: 1   MSVVGFDIGNENCVIAVVKQRGVDVLLNDESKRETPAVVCFGEKQRFLGSAGAASAMMNP 60

Query: 61  RSTISQVKRLIGRNFSEPDVQIELKMFPFKTSEALDGSILVHVKYLGETHTFTPVQIMGM 120
           +ST+SQVKRLIGR FSEPDVQ +L+M PF+TSEA DG IL+H+KYLGETHTFTPVQ+  M
Sbjct: 61  KSTVSQVKRLIGRKFSEPDVQRDLRMLPFQTSEAPDGGILIHLKYLGETHTFTPVQVTAM 120

Query: 121 LLAHLRDVAEKNLGAPFSDCVIGIPSYFTDLQRRLYWSAAVIAGLKPLRLMHDCTATALS 180
           L AHL+D+ EKN   P SDCVIGIPSYFTDLQRR Y  AA +AGLKPLRLMHDCTATALS
Sbjct: 121 LFAHLKDLIEKNQEIPISDCVIGIPSYFTDLQRRAYLDAATVAGLKPLRLMHDCTATALS 180

Query: 181 YGIYKTDFSNTGPIYVAFVDIGHCDTQVSIVSFEPGHMRIMSQTYDRDLGGRDFDEVLFS 240
           YGIYKTDF ++GP YVAFVDIGHCDTQV+I SFE G M+I+S T++R LGGRDFDEVLF 
Sbjct: 181 YGIYKTDFPSSGPTYVAFVDIGHCDTQVTIASFEAGQMKILSHTFERSLGGRDFDEVLFG 240

Query: 241 HFVAEFKKNYDIDVNSNVKASIRLRAACEKLKKVLSANLEAVLNIECLMDEKDVKGFIKR 300
           HF AEFK+ Y IDV SNVKASIRLRAACEKLKKVLSAN EA LNIECLMDEKDVKGFIKR
Sbjct: 241 HFAAEFKEQYRIDVYSNVKASIRLRAACEKLKKVLSANAEAPLNIECLMDEKDVKGFIKR 300

Query: 301 EEFEKLASGLLEKISIPCTRGLADAGLAVENIHSVELVGSGSRIPAISRLLTSVFKKEPS 360
           E+FE L+SGLLE+I +PC++ LADAGL  E IHSVELVGSGSRIPA+ R+LTSVF+KEP 
Sbjct: 301 EDFETLSSGLLERIGVPCSKALADAGLTAEKIHSVELVGSGSRIPAVGRVLTSVFRKEPR 360

Query: 361 RKLNASECVARGCALQCAMLSPVFRVREYEI 392
           R LNASECVARGCALQCAMLSPVFRVREYE+
Sbjct: 361 RTLNASECVARGCALQCAMLSPVFRVREYEV 391