Cucsa.049550.1 (mRNA) Cucumber (Gy14) v1

NameCucsa.049550.1
TypemRNA
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionRING/U-box superfamily protein
Locationscaffold00550 : 29154 .. 32519 (+)
Sequence length2477
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCTTCACCAGGACCACCAATATGCATTACTACTGGTGCCGAATTTGTCGAAGAATCATCAGAATTAGCTTAGGAAACCCTTTAGAAATTTCCATTACTTGCCCCTTTTGCTCCAGAAATCTCCGCCATGAATTGGACGTAGCCAGGGCTCGAAATTTCCCTCGTCTCCCCGCCATTTCCCCTCCGATTGTCCAACGGTGGGGATTCGAAGAAACCGATGCGTCATGGATCACGCTCCAATTCCCGAGACCAGCGGTAAATTCCGGGATTGAGGAGATTCCAAGGGTGAGAATTACGGGGAAGCATTTGGAGAAGGATTCGAATTGTGCGATTTGTAAAGAGGAATTTGAAATGGGAGAGGAAGTTAGGGAGTTGCCTTGCAAGCATTTTTACCATTCGGATTGTGTTGTTCCTTGGTTGAGAATGCATAACACTTGCCCTGTTTGCCGGTATACGTTGGAGAATATCGGCGACGAGGGGAGTGGGAGCGGTTTTAATGGCGGAAGGGGTGAGGAATTCCGGGAAGAAGAAGAAGAAGGTGAAAGAAGAGGGAATGGATGGTGGAACTTGGTGTGTTGCTGGTGGCCGTTCCGTGTAGTCGGCGATTGGGCTCGCCGTCTCCGGTTTGCTGATTCGGGTGATAGTTTCTTCGCCGTTGAACGTAAGGTTTTTtCTTTtCAAGTTTCTTCAGAGATGGATGGAGTTGGGAAACAAAATATAAATAACAAACCATTTTTTtATAATCAAAATGAAATCGTTGAGATTATTACAATTATTTTAAATTTTATCTTTAATTTTGTAATTTTAGATTAGATTACTGTGTTTTGAATTCTCTTGTAACCATTTATATTTTTtACTTTTGCTGAGAATTTTTCAAAAAAAAAAAaaTACAAACTACACAATTTATAAACACAACTCCGTATTTGATAAAAGATTAAATGTTAATTTGTATTGGTAGAGTTTGACATAAAATGTCTAATTTCAATCTATATACTTTGGTAGTTTAATATCTACTAGCATTCTAACCATAAATTTTAATAACACATTCACGTAAATATTATAAATATTATTATTATTGTCAAACCAAATAGAATAAAAAATAATTTTGAGAACTATATTAAAAATGTATTGAATACATTCAAACAAATACTAAAATATAATTTTCTTAAAAAaCAAAAAAGTGACAGAAATCCATGAAGATCCTTACACAAGACAACTAGATTCAAAATGACAAAATGGACCATCAAAATCAAACAAGTCAACTTAGAAAAGGACAAAATTTGACCATCAAAATCAAACTAATCAAATTCACAAAAACATAACTAAACTCTAACTCAAAACAATCAAATAGACAAAAAAAAAAATAATAAAAAAAAAAaGaGTTTGCAAAATCTAAATATTTAAATCTAATGTTAATTAATGTGTACTGGTTGGAGTTTGCAGCAGGTTCGTGGATGTACTCTTGGCTAGCCAATTAACCCACCCCCGGTAATATCTACTACATTCCTTACTCTTCATTTCCTTTCATTTCTTAACCAATTGATTAGGTACATGAAATTGTGTTGGATTTAGTAATGTAATTAACAAGCATTTTGCTTTCTTATTCCATTTCTTTCTTGTAAGTAACTACCAAAATTGCAGACAGTCAAAAGGAACCTTTGTTTAAGAACTGTACATCTTTTACTAAAGAAAATAAGACTATATAATAATAGTTCCTTTTGAGGTCTGCAACTTCAAATCTCTCCTTTCATAAATTAATTATATTTTTTtCTAAAACATATATAATTTAAACCCAACCAAGGAGGAGAGTTGGTTCCCTTTGGAAAACAACATCCCTATTTCCCTGCGTAGGGTTAATATAAAGGGAAATATCACCTTTTGTCTTCTTCTTATTCTTCTTCTTATTCTTCTTCGGACTCTCTATACTGACTTTCTTTGTTCTTGAGGAGTGAACCAAAAATGAGTTTCCCATCTGATTGTATTCTTCAGACTATTGTATGTTGATTATGTAGGTGATAGATGTAAATGAGTTGTAGGAGAGAGCGGTCAAGACCCGTTATTCTTAATCCTACAAAGATGAGAAGGGAATGAGTTGTGCTTTTGTAGATATAAATGAATTGATTTTGTACTAAGAAATACTACTACTACGTGCTTTCAACCTATAATCAAGTAAAAAATATGTCAAACCTTACATGAACTGGATGTGACGGATCTTCATTTGTCTGCCCTGATTTGGAGCGAAAAGAAGGCTTGGGTTTAGGTAACAGATCGGGGGGCAACAGTGGTGGAGACTCTACTGGAGATGCACGTGGAGAATAAACACGAGGTTGATTAGCTGGAGGAACGATCAGTCTGGTAGACTTAGGATACGAGATTGGTGGTATTGAATGTTCATGAGGGGAATTTGCAGGAACCATAGAAGGGTTCGGAGACGAGCTTTTGCAATTTGCATGAGGTGGGGAATGGTTTGCTGGTGGTCGCATGGATCTCAACGGATGTGGGCCACTCGGTACTTCTACATGATTGCCAGGCGCTGGGGCAGGGGCTGGAGAAAAACTTGGTGGCATTGCCTTGGAGGTTCTCTTCGGATAAGAAGACAAACTGACACTCTTGACTTCTCCAAAAACAGAATAATCAAGGCCAAGATTTCTTTCAGGAGAGGTGTTGATGATTGCAGCCAACTGCTGTAATCTCTGTGACGTAATGCGTCCCAATTCCGACGTAATAGAAGCCTGAACAATTACGAGTGGTTGCACTGTCGAGCCAATCTTGTTTGTTATTTGCAAATACACATTCTGTATAACAAGAAAAGAAAGAGTAGAATCCAACATCCATTAATCAACTCCAGTCCCAGATACATAAAACTAACAACAATAATATAAAACTCATCTCAAGTCTAAACTTATGATCTCAAGTTCTAGAGATAATATTTGATAAATTCCATCCATAGCAGAAAGTATAAGTTTAACTTAAATAAGTTTTTGTACCTCATAAGACCTCAGACGCAATCCAAACTTTAGCTGGCTCTTGAACTTGGCAAAGTTGTCAAGTATTTCGGAAATGGAGTTAGTAAGAGTGAAGTTAAATACGATCTGGGGAAACTCCCAAATCGAAGCATGTTGAAATGGGATTATAGAAATCCCCCCTGGAAACTTGAGAATTTGAAGTGTCGATGGCTGTCCAAAAATTGATGTCGTCAAAGTAAGGTTGGATTCCGAAAGGAAAAAGTCATATAAAGATGATCTCAGCAGACTTAAGGACACTGGATTTATTGGAGCAGTTATGTATTCAGAAAGAAGACCGAAAACCACGTAAGTCCTGTTTGACTCACCTATATCATGCATGGATAGAATGGAAACCT

mRNA sequence

ATGGCGGCTTCACCAGGACCACCAATATGCATTACTACTGCCAGGGCTCGAAATTTCCCTCGTCTCCCCGCCATTTCCCCTCCGATTGTCCAACGGTGGGGATTCGAAGAAACCGATGCGTCATGGATCACGCTCCAATTCCCGAGACCAGCGGTAAATTCCGGGATTGAGGAGATTCCAAGGGTGAGAATTACGGGGAAGCATTTGGAGAAGGATTCGAATTGTGCGATTTGTAAAGAGGAATTTGAAATGGGAGAGGAAGTTAGGGAGTTGCCTTGCAAGCATTTTTACCATTCGGATTGTGTTGTTCCTTGGTTGAGAATGCATAACACTTGCCCTGTTTGCCGGTATACGTTGGAGAATATCGGCGACGAGGGGAGTGGGAGCGGTTTTAATGGCGGAAGGGGTGAGGAATTCCGGGAAGAAGAAGAAGAAGGTGAAAGAAGAGGGAATGGATGGTGGAACTTGGTGTGTTGCTGGTGGCCGTTCCGTGTAGTCGGCGATTGGGCTCGCCGTCTCCGGTTTGCTGATTCGGGTGATAGTTTCTTCGCCGTTGAACCAGGTTCGTGGATGTACTCTTGGCTAGCCAATTAACCCACCCCCGGTAATATCTACTACATTCCTTACTCTTCATTTCCTTTCATTTCTTAACCAATTGATTAGGTACATGAAATTGTGTTGGATTTAGTAATGTAATTAACAAGCATTTTGCTTTCTTATTCCATTTCTTTCTTGTAAGTAACTACCAAAATTGCAGACAGTCAAAAGGAACCTTTGTTTAAGAACTGTACATCTTTTACTAAAGAAAATAAGACTATATAATAATAGTTCCTTTTGAGGTCTGCAACTTCAAATCTCTCCTTTCataaattaattatatttttttctaaaacatatataatttaaaCCCAACCAAGGAGGAGAGTTGGTTCCCTTTGGAAAACAACATCCCTATTTCCCTGCGTAGGGTTAATATAAAGGGAAATATCACCTTTTGtcttcttcttattcttcttcttattcttcttcGGACTCTCTATACTGACTTTCTTTGTTCTTGAGGAGTGAACCAAAAATGAGTTTCCCATCTGATTGTATTCTTCAGACTATTGTATGTTGATTATGTAGGTGATAGATGTAAATGAGTTGTAGGAGAGAGCGGTCAAGACCCGTTATTCTTAATCCTACAAAGATGAGAAGGGAATGAGTTGTGCTTTTGTAGATATAAATGAATTGATTTTGTACTAAGAAATACTACTACTACGTGCTTTCAACCTATAATCAAGTAAAAAATATGTCAAACCTTACATGAACTGGATGTGACGGATCTTCATTTGTCTGCCCTGATTTGGAGCGAAAAGAAGGCTTGGGTTTAGGTAACAGATCGGGGGGCAACAGTGGTGGAGACTCTACTGGAGATGCACGTGGAGAATAAACACGAGGTTGATTAGCTGGAGGAACGATCAGTCTGGTAGACTTAGGATACGAGATTGGTGGTATTGAATGTTCATGAGGGGAATTTGCAGGAACCATAGAAGGGTTCGGAGACGAGCTTTTGCAATTTGCATGAGGTGGGGAATGGTTTGCTGGTGGTCGCATGGATCTCAACGGATGTGGGCCACTCGGTACTTCTACATGATTGCCAGGCGCTGGGGCAGGGGCTGGAGAAAAACTTGGTGGCATTGCCTTGGAGGTTCTCTTCGGATAAGAAGACAAACTGACACTCTTGACTTCTCCAAAAACAGAATAATCAAGGCCAAGATTTCTTTCAGGAGAGGTGTTGATGATTGCAGCCAACTGCTGTAATCTCTGTGACGTAATGCGTCCCAATTCCGACGTAATAGAAGCCTGAACAATTACGAGTGGTTGCACTGTCGAGCCAATCTTGTTTGTTATTTGCAAATACACATTCTGTATAACAAGAAAAGAAAGAGTAGAATCCAACATCCATTAATCAACTCCAGTCCCAGATACATAAAACTAACAACAATAATATAAAACTCATCTCAAGTCTAAACTTATGATCTCAAGTTCTAGAGATAATATTTGATAAATTCCATCCATAGCAGAAAGTATAAGTTTAACTTAAATAAGTTTTTGTACCTCATAAGACCTCAGACGCAATCCAAACTTTAGCTGGCTCTTGAACTTGGCAAAGTTGTCAAGTATTTCGGAAATGGAGTTAGTAAGAGTGAAGTTAAATACGATCTGGGGAAACTCCCAAATCGAAGCATGTTGAAATGGGATTATAGAAATCCCCCCTGGAAACTTGAGAATTTGAAGTGTCGATGGCTGTCCAAAAATTGATGTCGTCAAAGTAAGGTTGGATTCCGAAAGGAAAAAGTCATATAAAGATGATCTCAGCAGACTTAAGGACACTGGATTTATTGGAGCAGTTATGTATTCAGAAAGAAGACCGAAAACCACGTAAGTCCTGTTTGACTCACCTATATCATGCATGGATAGAATGGAAACCT

Coding sequence (CDS)

ATGGCGGCTTCACCAGGACCACCAATATGCATTACTACTGCCAGGGCTCGAAATTTCCCTCGTCTCCCCGCCATTTCCCCTCCGATTGTCCAACGGTGGGGATTCGAAGAAACCGATGCGTCATGGATCACGCTCCAATTCCCGAGACCAGCGGTAAATTCCGGGATTGAGGAGATTCCAAGGGTGAGAATTACGGGGAAGCATTTGGAGAAGGATTCGAATTGTGCGATTTGTAAAGAGGAATTTGAAATGGGAGAGGAAGTTAGGGAGTTGCCTTGCAAGCATTTTTACCATTCGGATTGTGTTGTTCCTTGGTTGAGAATGCATAACACTTGCCCTGTTTGCCGGTATACGTTGGAGAATATCGGCGACGAGGGGAGTGGGAGCGGTTTTAATGGCGGAAGGGGTGAGGAATTCCGGGAAGAAGAAGAAGAAGGTGAAAGAAGAGGGAATGGATGGTGGAACTTGGTGTGTTGCTGGTGGCCGTTCCGTGTAGTCGGCGATTGGGCTCGCCGTCTCCGGTTTGCTGATTCGGGTGATAGTTTCTTCGCCGTTGAACCAGGTTCGTGGATGTACTCTTGGCTAGCCAATTAA

Protein sequence

MAASPGPPICITTARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLEKDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLRMHNTCPVCRYTLENIGDEGSGSGFNGGRGEEFREEEEEGERRGNGWWNLVCCWWPFRVVGDWARRLRFADSGDSFFAVEPGSWMYSWLAN*
BLAST of Cucsa.049550.1 vs. Swiss-Prot
Match: RHC1A_ARATH (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana GN=RHC1A PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 3.5e-23
Identity = 54/141 (38.30%), Postives = 73/141 (51.77%), Query Frame = 1

Query: 35  FEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLEK-DSNCAICKEEFEMGEEVRELPC 94
           FE+  A   T + P PA  S I+ +P ++I  +HL   DSNC +CK+EFE+G E +++PC
Sbjct: 150 FEQLSAG-TTRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPC 209

Query: 95  KHFYHSDCVVPWLRMHNTCPVCRYTLENIGDEGSGS-------GFNGGRGEEFREEEEEG 154
            H YHSDC+VPWL  HN+CPVCR  L +     S          +            E G
Sbjct: 210 NHIYHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTRNYRSSSSSSSSNSRENG 269

Query: 155 ERRGNGWWNLVCCWWPFRVVG 168
             R N +      +WPFR  G
Sbjct: 270 NERRNPF----SSFWPFRSSG 285

BLAST of Cucsa.049550.1 vs. Swiss-Prot
Match: RDUF1_ARATH (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 8.1e-20
Identity = 50/114 (43.86%), Postives = 64/114 (56.14%), Query Frame = 1

Query: 50  PAVNSGIEEIPRVRITGKHLEKDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLRMH 109
           PA  S IE +PRV I+  H + ++NCA+C E FE G E RE+PCKH +H DC+VPWL + 
Sbjct: 190 PASKSAIESLPRVEISDCHTKAEANCAVCTEVFEAGIEGREMPCKHIFHGDCIVPWLSIR 249

Query: 110 NTCPVCRYTLENIGDEGS---------------GSGFNGGRGEEFREEEEEGER 149
           N+CPVCR+ L +   + S               G GF  GR   F     EGER
Sbjct: 250 NSCPVCRFELPSDPIQRSNEEEHAVGMTIWRLPGGGFAVGR---FNAGVREGER 300

BLAST of Cucsa.049550.1 vs. Swiss-Prot
Match: RING1_GOSHI (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.4e-19
Identity = 46/124 (37.10%), Postives = 68/124 (54.84%), Query Frame = 1

Query: 50  PAVNSGIEEIPRVRITGKHLEKDSN-CAICKEEFEMGEEVRELPCKHFYHSDCVVPWLRM 109
           PA  S IE +P V IT  +L  + N CA+C ++FE G E +++PCKH YH DC++PWL +
Sbjct: 198 PASKSAIEALPLVNITKSNLNSEFNQCAVCMDDFEEGTEAKQMPCKHLYHKDCLLPWLEL 257

Query: 110 HNTCPVCRYTLENIGDE-----GSGSGFNGGRGEEFREEEEEGERRGNGWWNLVCCWWPF 168
           HN+CPVCR+ L     +         G +GG   +   +  +G+ R       +   WPF
Sbjct: 258 HNSCPVCRHELPTDDPDYERRVRGAQGTSGGNDGDNSGQRSDGDNRTVERSFRISLPWPF 317

BLAST of Cucsa.049550.1 vs. Swiss-Prot
Match: RDUF2_ARATH (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana GN=DURF2 PE=1 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.8e-19
Identity = 52/129 (40.31%), Postives = 68/129 (52.71%), Query Frame = 1

Query: 37  ETDASWITLQFPRPAVNSGIEEIPRVRITGKHLEKDSNCAICKEEFEMGEEVRELPCKHF 96
           E  A+ I      PA  S IE +PRV I+  H+  ++NCA+C E FE   E RE+PCKH 
Sbjct: 161 EASATGIGRSGNPPASKSAIESLPRVEISDCHIGSEANCAVCTEIFETETEAREMPCKHL 220

Query: 97  YHSDCVVPWLRMHNTCPVCRYTL-------ENIGDEGS----------GSGFNGGRGEEF 149
           +H DC+VPWL + N+CPVCR+ L        N  +E +          G GF  GR   F
Sbjct: 221 FHDDCIVPWLSIRNSCPVCRFELPSEPNRRSNNNEEDNAVGMTIWRLPGGGFAVGR---F 280

BLAST of Cucsa.049550.1 vs. Swiss-Prot
Match: RNG1L_ARATH (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 3.1e-19
Identity = 41/99 (41.41%), Postives = 63/99 (63.64%), Query Frame = 1

Query: 50  PAVNSGIEEIPRVRITGKHLEKDSN-CAICKEEFEMGEEVRELPCKHFYHSDCVVPWLRM 109
           PA  S I+ +P V++T   L+ + N CA+C +EFE G +V+++PCKH +H DC++PWL +
Sbjct: 190 PASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLEL 249

Query: 110 HNTCPVCRYTL---------ENIGDEGSGSGFNGGRGEE 139
           HN+CPVCR+ L          + G +GSG G     G++
Sbjct: 250 HNSCPVCRFELPTDDPDYENRSQGSQGSGDGQGSVEGQQ 288

BLAST of Cucsa.049550.1 vs. TrEMBL
Match: A0A0A0LC10_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G214060 PE=4 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 1.2e-102
Identity = 173/182 (95.05%), Postives = 176/182 (96.70%), Query Frame = 1

Query: 11  ITTARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE 70
           +  ARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE
Sbjct: 62  LDVARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE 121

Query: 71  KDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLRMHNTCPVCRYTLENIGDEGSGSG 130
           KDSNCAICKEEFEMGEEVRELPCKHFYHSDCV+PWLRMHNTCPVCRYTLENIGDEGSGSG
Sbjct: 122 KDSNCAICKEEFEMGEEVRELPCKHFYHSDCVIPWLRMHNTCPVCRYTLENIGDEGSGSG 181

Query: 131 FNGGRGEEFR-EEEEEGERRGNGWWNLVCCWWPFRVVGDWARRLRFADSGDSFFAVEPGS 190
           FNGGRGEEFR EEEEEGERRGNGWWNLVCCWWPFR+VGDWARRLRFADSGDSFFAVE   
Sbjct: 182 FNGGRGEEFREEEEEEGERRGNGWWNLVCCWWPFRLVGDWARRLRFADSGDSFFAVERKR 241

Query: 191 WM 192
           WM
Sbjct: 242 WM 243

BLAST of Cucsa.049550.1 vs. TrEMBL
Match: A0A068TNL0_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00014914001 PE=4 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 3.9e-29
Identity = 62/125 (49.60%), Postives = 79/125 (63.20%), Query Frame = 1

Query: 48  PRPAVNSGIEEIPRVRITGKHLEKDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLR 107
           P PA  S I+ +P + +T  HL  DS+C +CK+EFE GEEVRELPCKHFYHSDC++PWL 
Sbjct: 157 PTPA--SAIDALPTIVLTPTHLAIDSHCPVCKDEFEAGEEVRELPCKHFYHSDCIIPWLH 216

Query: 108 MHNTCPVCRYTLENIGDEGSGSGFNGGRGEEFREEEEEGERRGNGWWNLVCCWWPFRVVG 167
           + N+CPVCRY +  +    S S F   + + F+EEEE        W   V   WPF + G
Sbjct: 217 IRNSCPVCRYQIRGL----SNSDFREEQADNFQEEEEVSSSPHRNWLLRVFSLWPFSLFG 275

Query: 168 DWARR 173
           DWA R
Sbjct: 277 DWAYR 275

BLAST of Cucsa.049550.1 vs. TrEMBL
Match: M1C6Z4_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400023756 PE=4 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.1e-28
Identity = 62/128 (48.44%), Postives = 81/128 (63.28%), Query Frame = 1

Query: 48  PRPAVNSGIEEIPRVRITGKHLEKDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLR 107
           P P  +S I+ +PR+ +T   LE DS C +CK+EFE+G EV ELPCKHFYHS+C+VPWLR
Sbjct: 150 PPPTPSSAIDALPRIYLTPNLLENDSVCPVCKDEFEVGIEVIELPCKHFYHSECIVPWLR 209

Query: 108 MHNTCPVCRYTLENIGDEGSGSGFNGGRGEEFREEEEEGERRGNG--W-WNLVCCWWPFR 167
           +HNTCPVCRY L+   +    +G+      EF E+EEE E   N   W W  +   WPF 
Sbjct: 210 LHNTCPVCRYQLQGFSNNEDQNGYT----YEFNEQEEEEENIQNPLIWGWTQLTSLWPFS 269

Query: 168 VVGDWARR 173
           ++  W  R
Sbjct: 270 LMSSWRHR 273

BLAST of Cucsa.049550.1 vs. TrEMBL
Match: A0A0B2RNN4_GLYSO (E3 ubiquitin-protein ligase RING1 OS=Glycine soja GN=glysoja_005509 PE=4 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 7.4e-28
Identity = 60/125 (48.00%), Postives = 75/125 (60.00%), Query Frame = 1

Query: 48  PRPAVNSGIEEIPRVRITGKHLEKDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLR 107
           P PA +S I  +P V++T  HL  D NC ICK+EFE+  E RELPCKHFYHSDC++PWLR
Sbjct: 172 PPPATSSAIAALPMVKLTQTHLASDPNCPICKDEFELDMEARELPCKHFYHSDCIIPWLR 231

Query: 108 MHNTCPVCRYTLENIGDEGSGSGFNGGRGEEFREEEEEGERRGNGW-WNLVCCWWPFRVV 167
           MHNTCPVCRY L+ +   G+ + +     E       E       W WN +  + P R V
Sbjct: 232 MHNTCPVCRYELQGVSTSGNANYYRLQNDENDMRFGFEDAASSLVWVWNQLTSFRPIRSV 291

Query: 168 GDWAR 172
            DW R
Sbjct: 292 LDWTR 296

BLAST of Cucsa.049550.1 vs. TrEMBL
Match: K7M9N3_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_15G047300 PE=4 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 7.4e-28
Identity = 60/125 (48.00%), Postives = 75/125 (60.00%), Query Frame = 1

Query: 48  PRPAVNSGIEEIPRVRITGKHLEKDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLR 107
           P PA +S I  +P V++T  HL  D NC ICK+EFE+  E RELPCKHFYHSDC++PWLR
Sbjct: 172 PPPATSSAIAALPMVKLTQTHLASDPNCPICKDEFELDMEARELPCKHFYHSDCIIPWLR 231

Query: 108 MHNTCPVCRYTLENIGDEGSGSGFNGGRGEEFREEEEEGERRGNGW-WNLVCCWWPFRVV 167
           MHNTCPVCRY L+ +   G+ + +     E       E       W WN +  + P R V
Sbjct: 232 MHNTCPVCRYELQGVSTSGNANYYRLQNDENDMRFGFEDAASSLVWVWNQLTSFRPIRSV 291

Query: 168 GDWAR 172
            DW R
Sbjct: 292 LDWTR 296

BLAST of Cucsa.049550.1 vs. TAIR10
Match: AT1G68180.1 (AT1G68180.1 RING/U-box superfamily protein)

HSP 1 Score: 112.1 bits (279), Expect = 4.0e-25
Identity = 57/132 (43.18%), Postives = 71/132 (53.79%), Query Frame = 1

Query: 48  PRPAVNSGIEEIPRVRITGKHLEKDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLR 107
           P PA  S IE +  V IT + L K+  CAICKEEFE+GEE +EL C H YHS C+V WL 
Sbjct: 111 PPPASQSAIEAVRTVIITDEDLVKEKVCAICKEEFEVGEEGKELKCLHLYHSSCIVSWLN 170

Query: 108 MHNTCPVCRYTLENIG------DEGSGSGFNGGRGEEFREEEEEGERRGNGWWNLVCCWW 167
           +HNTCP+CR+ + N+G      DEG     +  R   FR                VC  W
Sbjct: 171 IHNTCPICRFEV-NLGVSESNVDEGGSYNIDNDRSNRFRTR--------------VCSLW 227

Query: 168 PFRVVGDWARRL 174
           P R++ DW   L
Sbjct: 231 PLRMMIDWVHNL 227

BLAST of Cucsa.049550.1 vs. TAIR10
Match: AT2G40830.2 (AT2G40830.2 RING-H2 finger C1A)

HSP 1 Score: 109.8 bits (273), Expect = 2.0e-24
Identity = 54/141 (38.30%), Postives = 73/141 (51.77%), Query Frame = 1

Query: 35  FEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLEK-DSNCAICKEEFEMGEEVRELPC 94
           FE+  A   T + P PA  S I+ +P ++I  +HL   DSNC +CK+EFE+G E +++PC
Sbjct: 150 FEQLSAG-TTRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPC 209

Query: 95  KHFYHSDCVVPWLRMHNTCPVCRYTLENIGDEGSGS-------GFNGGRGEEFREEEEEG 154
            H YHSDC+VPWL  HN+CPVCR  L +     S          +            E G
Sbjct: 210 NHIYHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTRNYRSSSSSSSSNSRENG 269

Query: 155 ERRGNGWWNLVCCWWPFRVVG 168
             R N +      +WPFR  G
Sbjct: 270 NERRNPF----SSFWPFRSSG 285

BLAST of Cucsa.049550.1 vs. TAIR10
Match: AT3G56580.2 (AT3G56580.2 RING/U-box superfamily protein)

HSP 1 Score: 107.8 bits (268), Expect = 7.6e-24
Identity = 52/118 (44.07%), Postives = 69/118 (58.47%), Query Frame = 1

Query: 48  PRPAVNSGIEEIPRVRITGKHLEK-DSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWL 107
           P PA  S I+ +P ++IT KHL+  DS+C +CK+EFE+  E +++PC H YHSDC+VPWL
Sbjct: 158 PPPAPKSSIDALPTIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWL 217

Query: 108 RMHNTCPVCRYTLENIGDEGSGSGFNGGRGEEFREEEEEGERRGNGWWNLVCCWWPFR 165
             HN+CPVCR  L + G   S         +       E  RR N + NL    WPFR
Sbjct: 218 VQHNSCPVCRKELPSRGSSSSTQS-----SQNRSTNGRENSRRRNIFSNL----WPFR 266

BLAST of Cucsa.049550.1 vs. TAIR10
Match: AT3G46620.1 (AT3G46620.1 zinc finger (C3HC4-type RING finger) family protein)

HSP 1 Score: 98.6 bits (244), Expect = 4.6e-21
Identity = 50/114 (43.86%), Postives = 64/114 (56.14%), Query Frame = 1

Query: 50  PAVNSGIEEIPRVRITGKHLEKDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLRMH 109
           PA  S IE +PRV I+  H + ++NCA+C E FE G E RE+PCKH +H DC+VPWL + 
Sbjct: 190 PASKSAIESLPRVEISDCHTKAEANCAVCTEVFEAGIEGREMPCKHIFHGDCIVPWLSIR 249

Query: 110 NTCPVCRYTLENIGDEGS---------------GSGFNGGRGEEFREEEEEGER 149
           N+CPVCR+ L +   + S               G GF  GR   F     EGER
Sbjct: 250 NSCPVCRFELPSDPIQRSNEEEHAVGMTIWRLPGGGFAVGR---FNAGVREGER 300

BLAST of Cucsa.049550.1 vs. TAIR10
Match: AT5G59550.1 (AT5G59550.1 zinc finger (C3HC4-type RING finger) family protein)

HSP 1 Score: 97.4 bits (241), Expect = 1.0e-20
Identity = 52/129 (40.31%), Postives = 68/129 (52.71%), Query Frame = 1

Query: 37  ETDASWITLQFPRPAVNSGIEEIPRVRITGKHLEKDSNCAICKEEFEMGEEVRELPCKHF 96
           E  A+ I      PA  S IE +PRV I+  H+  ++NCA+C E FE   E RE+PCKH 
Sbjct: 161 EASATGIGRSGNPPASKSAIESLPRVEISDCHIGSEANCAVCTEIFETETEAREMPCKHL 220

Query: 97  YHSDCVVPWLRMHNTCPVCRYTL-------ENIGDEGS----------GSGFNGGRGEEF 149
           +H DC+VPWL + N+CPVCR+ L        N  +E +          G GF  GR   F
Sbjct: 221 FHDDCIVPWLSIRNSCPVCRFELPSEPNRRSNNNEEDNAVGMTIWRLPGGGFAVGR---F 280

BLAST of Cucsa.049550.1 vs. NCBI nr
Match: gi|449464484|ref|XP_004149959.1| (PREDICTED: E3 ubiquitin-protein ligase RNF126 isoform X1 [Cucumis sativus])

HSP 1 Score: 402.9 bits (1034), Expect = 3.2e-109
Identity = 182/188 (96.81%), Postives = 185/188 (98.40%), Query Frame = 1

Query: 11  ITTARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE 70
           +  ARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE
Sbjct: 62  LDVARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE 121

Query: 71  KDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLRMHNTCPVCRYTLENIGDEGSGSG 130
           KDSNCAICKEEFEMGEEVRELPCKHFYHSDCV+PWLRMHNTCPVCRYTLENIGDEGSGSG
Sbjct: 122 KDSNCAICKEEFEMGEEVRELPCKHFYHSDCVIPWLRMHNTCPVCRYTLENIGDEGSGSG 181

Query: 131 FNGGRGEEFR-EEEEEGERRGNGWWNLVCCWWPFRVVGDWARRLRFADSGDSFFAVEPGS 190
           FNGGRGEEFR EEEEEGERRGNGWWNLVCCWWPFR+VGDWARRLRFADSGDSFFAVEPGS
Sbjct: 182 FNGGRGEEFREEEEEEGERRGNGWWNLVCCWWPFRLVGDWARRLRFADSGDSFFAVEPGS 241

Query: 191 WMYSWLAN 198
           WMYSWLAN
Sbjct: 242 WMYSWLAN 249

BLAST of Cucsa.049550.1 vs. NCBI nr
Match: gi|778680175|ref|XP_011651263.1| (PREDICTED: E3 ubiquitin-protein ligase RNF126 isoform X2 [Cucumis sativus])

HSP 1 Score: 392.5 bits (1007), Expect = 4.4e-106
Identity = 180/188 (95.74%), Postives = 183/188 (97.34%), Query Frame = 1

Query: 11  ITTARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE 70
           +  ARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE
Sbjct: 62  LDVARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE 121

Query: 71  KDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLRMHNTCPVCRYTLENIGDEGSGSG 130
           KDSNCAICKEEFEMGEEVRELPCKHFYHSDCV+PWLRMHNTCPVCRYTLENIGDEGSGSG
Sbjct: 122 KDSNCAICKEEFEMGEEVRELPCKHFYHSDCVIPWLRMHNTCPVCRYTLENIGDEGSGSG 181

Query: 131 FNGGRGEEFR-EEEEEGERRGNGWWNLVCCWWPFRVVGDWARRLRFADSGDSFFAVEPGS 190
           FNGGRGEEFR EEEEEGERRGNGWWNLVCCWWPFR+VGDWARRLRFADSGDSFFAVE  S
Sbjct: 182 FNGGRGEEFREEEEEEGERRGNGWWNLVCCWWPFRLVGDWARRLRFADSGDSFFAVE-RS 241

Query: 191 WMYSWLAN 198
           WMYSWLAN
Sbjct: 242 WMYSWLAN 248

BLAST of Cucsa.049550.1 vs. NCBI nr
Match: gi|659112139|ref|XP_008456082.1| (PREDICTED: uncharacterized protein LOC103496124 isoform X1 [Cucumis melo])

HSP 1 Score: 384.8 bits (987), Expect = 9.1e-104
Identity = 174/192 (90.62%), Postives = 178/192 (92.71%), Query Frame = 1

Query: 11  ITTARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE 70
           +  ARARNFPRLP ISPPIVQRWGFE+TD SWITLQFPRPAVNSGIEEIPRVRITGKHLE
Sbjct: 62  LDVARARNFPRLPPISPPIVQRWGFEDTDESWITLQFPRPAVNSGIEEIPRVRITGKHLE 121

Query: 71  KDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLRMHNTCPVCRYTLENIGDEGSGSG 130
           KDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLRMHNTCPVCRYTLENIGDEGSGSG
Sbjct: 122 KDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLRMHNTCPVCRYTLENIGDEGSGSG 181

Query: 131 FNGGRGEEFREEEE-----EGERRGNGWWNLVCCWWPFRVVGDWARRLRFADSGDSFFAV 190
           FNGGRGEE  EEEE     EGE RGNGWWNLVCCWWP R++GDWARRLRFADSGDSFF V
Sbjct: 182 FNGGRGEELGEEEEEEEEGEGEGRGNGWWNLVCCWWPLRLIGDWARRLRFADSGDSFFVV 241

Query: 191 EPGSWMYSWLAN 198
           EPGSWMYSWLAN
Sbjct: 242 EPGSWMYSWLAN 253

BLAST of Cucsa.049550.1 vs. NCBI nr
Match: gi|778680186|ref|XP_011651266.1| (PREDICTED: E3 ubiquitin-protein ligase RNF126 isoform X3 [Cucumis sativus])

HSP 1 Score: 381.3 bits (978), Expect = 1.0e-102
Identity = 173/179 (96.65%), Postives = 176/179 (98.32%), Query Frame = 1

Query: 11  ITTARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE 70
           +  ARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE
Sbjct: 62  LDVARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE 121

Query: 71  KDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLRMHNTCPVCRYTLENIGDEGSGSG 130
           KDSNCAICKEEFEMGEEVRELPCKHFYHSDCV+PWLRMHNTCPVCRYTLENIGDEGSGSG
Sbjct: 122 KDSNCAICKEEFEMGEEVRELPCKHFYHSDCVIPWLRMHNTCPVCRYTLENIGDEGSGSG 181

Query: 131 FNGGRGEEFR-EEEEEGERRGNGWWNLVCCWWPFRVVGDWARRLRFADSGDSFFAVEPG 189
           FNGGRGEEFR EEEEEGERRGNGWWNLVCCWWPFR+VGDWARRLRFADSGDSFFAVEPG
Sbjct: 182 FNGGRGEEFREEEEEEGERRGNGWWNLVCCWWPFRLVGDWARRLRFADSGDSFFAVEPG 240

BLAST of Cucsa.049550.1 vs. NCBI nr
Match: gi|700202433|gb|KGN57566.1| (hypothetical protein Csa_3G214060 [Cucumis sativus])

HSP 1 Score: 380.6 bits (976), Expect = 1.7e-102
Identity = 173/182 (95.05%), Postives = 176/182 (96.70%), Query Frame = 1

Query: 11  ITTARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE 70
           +  ARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE
Sbjct: 62  LDVARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE 121

Query: 71  KDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLRMHNTCPVCRYTLENIGDEGSGSG 130
           KDSNCAICKEEFEMGEEVRELPCKHFYHSDCV+PWLRMHNTCPVCRYTLENIGDEGSGSG
Sbjct: 122 KDSNCAICKEEFEMGEEVRELPCKHFYHSDCVIPWLRMHNTCPVCRYTLENIGDEGSGSG 181

Query: 131 FNGGRGEEFR-EEEEEGERRGNGWWNLVCCWWPFRVVGDWARRLRFADSGDSFFAVEPGS 190
           FNGGRGEEFR EEEEEGERRGNGWWNLVCCWWPFR+VGDWARRLRFADSGDSFFAVE   
Sbjct: 182 FNGGRGEEFREEEEEEGERRGNGWWNLVCCWWPFRLVGDWARRLRFADSGDSFFAVERKR 241

Query: 191 WM 192
           WM
Sbjct: 242 WM 243

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
RHC1A_ARATH3.5e-2338.30Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana GN=RHC1A PE=2... [more]
RDUF1_ARATH8.1e-2043.86E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana GN=RDUF1 PE=1 SV=1[more]
RING1_GOSHI1.4e-1937.10E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1[more]
RDUF2_ARATH1.8e-1940.31E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana GN=DURF2 PE=1 SV=1[more]
RNG1L_ARATH3.1e-1941.41E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1... [more]
Match NameE-valueIdentityDescription
A0A0A0LC10_CUCSA1.2e-10295.05Uncharacterized protein OS=Cucumis sativus GN=Csa_3G214060 PE=4 SV=1[more]
A0A068TNL0_COFCA3.9e-2949.60Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00014914001 PE=4 SV=1[more]
M1C6Z4_SOLTU1.1e-2848.44Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400023756 PE=4 SV=1[more]
A0A0B2RNN4_GLYSO7.4e-2848.00E3 ubiquitin-protein ligase RING1 OS=Glycine soja GN=glysoja_005509 PE=4 SV=1[more]
K7M9N3_SOYBN7.4e-2848.00Uncharacterized protein OS=Glycine max GN=GLYMA_15G047300 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G68180.14.0e-2543.18 RING/U-box superfamily protein[more]
AT2G40830.22.0e-2438.30 RING-H2 finger C1A[more]
AT3G56580.27.6e-2444.07 RING/U-box superfamily protein[more]
AT3G46620.14.6e-2143.86 zinc finger (C3HC4-type RING finger) family protein[more]
AT5G59550.11.0e-2040.31 zinc finger (C3HC4-type RING finger) family protein[more]
Match NameE-valueIdentityDescription
gi|449464484|ref|XP_004149959.1|3.2e-10996.81PREDICTED: E3 ubiquitin-protein ligase RNF126 isoform X1 [Cucumis sativus][more]
gi|778680175|ref|XP_011651263.1|4.4e-10695.74PREDICTED: E3 ubiquitin-protein ligase RNF126 isoform X2 [Cucumis sativus][more]
gi|659112139|ref|XP_008456082.1|9.1e-10490.63PREDICTED: uncharacterized protein LOC103496124 isoform X1 [Cucumis melo][more]
gi|778680186|ref|XP_011651266.1|1.0e-10296.65PREDICTED: E3 ubiquitin-protein ligase RNF126 isoform X3 [Cucumis sativus][more]
gi|700202433|gb|KGN57566.1|1.7e-10295.05hypothetical protein Csa_3G214060 [Cucumis sativus][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR001841Znf_RING
IPR013083Znf_RING/FYVE/PHD
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
GO:0008270zinc ion binding
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005515 protein binding
molecular_function GO:0008270 zinc ion binding

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsa.049550Cucsa.049550gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsa.049550.1Cucsa.049550.1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.049550.1.CDS.1Cucsa.049550.1.CDS.1CDS
Cucsa.049550.1.CDS.2Cucsa.049550.1.CDS.2CDS
Cucsa.049550.1.CDS.3Cucsa.049550.1.CDS.3CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.049550.1.three_prime_UTR.1Cucsa.049550.1.three_prime_UTR.1three_prime_UTR


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 74..116
score: 6.7
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 75..115
score: 2.
IPR001841Zinc finger, RING-typePROFILEPS50089ZF_RING_2coord: 75..116
score: 12
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 70..119
score: 1.2
NoneNo IPR availablePANTHERPTHR22763RING ZINC FINGER PROTEINcoord: 48..153
score: 6.6
NoneNo IPR availablePANTHERPTHR22763:SF88RING/U-BOX DOMAIN-CONTAINING PROTEINcoord: 48..153
score: 6.6
NoneNo IPR availableunknownSSF57850RING/U-boxcoord: 56..120
score: 2.14