Cucsa.036060.1 (mRNA) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.036060.1
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Fatty-acid desaturase
Location	scaffold00464 : 9119 .. 11769 (+)
Sequence length	843

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATACAATGGTGAAGGCTGATCAAATCCCCTTTGGGGAGAGAAAATGGACAAACAGAGACAAGTATATGGCAGCTCTTTTTGTTATTATGCACTTCATTTGTATTCTTGCACCCTTCCATTTCAATTGGAATGCATTTTGGGTCGCTACTCTGTTATACTTCTTCAGTTTTTTTtGGAATCAATATTTCGTATCATAGAAATCTTTCACATAGAAGTTTCAGACTCTCCAAATGGCTCGAATACTTTTTTGCATATTGTGGAGCTCTCGCTTTTCAGGTTTGATATTCTTAATACTTTCTTCTTTCTTTCTTATTTGACATTAAAAAAATATTGAGAGACGTTCTAATGTATCTACTTTTGTAAAGTTTCAATGTTATATTCTAAAAGAAAaTAAAACATATTATACTTACTGTTTTtCTTTTTttTTtttAATTTTAGGGTGATCCAaTCGATTGGGTGAGTACACATCGATGTCATCATCAATTTGTCGATACAGAAaATGATCCGCACAGTCCTATTCAAGGATTTTGGTTTAGTTATTTCACTTGGATTTTGGATTCGAATGCTTTGACTAAAAGAGTTTGTCCACAGTATTTTATTGATCATAAAGATACAGATAAAACCATCTTCACATTGGTTTTAAAGTATGGAAGACCAAATAATGTTGGTGACTTGGAGAAACAGTCCTTCTATAGGTTTCTTCGCAAAACTTATTTTCTTCATCAACTTGCTCTTGCAATCCTTCTTTATGCGGTTGGAGGAAAACCTTTTCTTGTATGGGGAATGGTAAAATAAAATTACTTATTTATTTATTAACAAAATTAATCAAAATATTTATAGATATATAATAAAATACTATTGTTATCAACAATGAAATAGTTATATACACATATAAACTACAATTATCATCTGCCACTAATAATAGACGACAGCGATAGACATCAATCAATGTTATTCATTTATAAACAATGACATAATTTTATTATATTTCTATATATTTACAACGTTATTATCGCTTGAAACAAGTATTTTTATATTTGAATATATATTTTTATTTGAACTTATAAATGAAGCAAGCATATGATACTTTATATTATTATAAATAAGATTCATTGTTTTGCAGTTTGTGAGGACCATAGCATTCCTACATGTGACGTTTATGTTGAATTCAATATGTCATACATTTGGAAATCAACCATGGAACACTGGAGATTTGTCTAAAAATACTTGGTATAAGTGCTTTTGTATATATATATTTTTTCATTTTATCTTCATCTTCCTATAGTTGTGACCTTTATTTTAGTTAGCTAGTAATATTCTTTAAATACAGTAAATTTATGTGGTTAATATATGCCAACCAAATTAAGAAAAACATTTTAAAAAGTAGTTTCTTAAACAAAGTATAAATATAAGTCATTTATAAAATAAACGGCTACTAATCATATTAGTTAACTTGTGTATATAATTAGTTCAATAATATGGTATCTGAAAAATTCATTAGTCAACAAATAATACAATAAATGCGAGGCTTTGATAGTATCAATGATTAATCTTTAAAAAAaTTATCAATGATTAATCTTTAAAAAAaTTATCAATGATATCTTCCAATGTCATAACTGTAGTTAGTGGTAGAAAAATTGGTTATCTAACTCTTTTAAAATTGCAAATAATCTTTCATATATCTCTAATTGTAGTTTAAAAGGAAAAAGAAATCAGCATATATTAACTAGTTTATGTCTTGAATGGTTCATAGTTACGTAACCCATAACAGTTCACACTTCGAAAAAAGCCACCTAAAAGGTTTAAGGGTTGGGAGGAGAGCATAAAAATATGAGAGTTCTCGGACTAACTTGCAATAGTTAAAAGTTCTTCCCTCATCGGTAGGCTGTTGCTTCGTTCAAATATTCAGGTACTTGTACTGAGGCGAAAATGAGAGAAACACGTATTTTTGTTTATTTTTATTCTTTTTCTTTCTTTAAATGTTATGAAATTAAACTTGGATCTGTAATTTCAATTATATAATAACTAAATCTAAACATTTTATTATTTTATTGATTCTAATTCCATCTTCCTAATCATTCTTTTTTtCTTTTTTTtAATAAAGAAGAGCTTTTCCTCTATTTCAATTCCTTCTCCGACGAATGCACGTTCGAAGTGGGTATTGTGTTAACTTTAGAGAGCAATTGACATAAATGATTCCCACCAAATTTGCTTTCAAGATAATAATTTCCACAACACGAAGCTTTACATCCATATTAGACCAGATGACATATATAGCATATATATCATTAGTGAATTGGAATAGACAATCGTTGAATGTTGTGTTTGTAAAGTAAACAAAGCTAACTCCACATTATAACATTATAGCTAAAGCCTTTCTATGTTGCAAATTAATTAATTAACACAATATTTTTtGTTTGTTTTTAGGTGGATGTGTTTGCTTACACTTGGAGAAAGTTGGCATAATAACCATCATGCATTCGAGTATTCAGCCAGACAAGGGCTTGAATGGTGGCAGATCGACATTTGTTGGTACATTATTTGGTTTCTTCAAGTCATTGGATTAGCAACTGAGGTTAAAGTACCCTCTCAATCTCACAAGCAAAGACTACAAGCTTTGGACCAAACCAAAAGAAAAGAACTTTGA

mRNA sequence

ATGGATACAATGGTGAAGGCTGATCAAATCCCCTTTGGGGAGAGAAAATGGACAAACAGAGACAAGTATATGGCAGCTCTTTTTGTTATTATGCACTTCATTTGTATTCTTGCACCCTTCCATTTCAATTGGAATGCATTTTGGGTCGCTACTCTAAATCTTTCACATAGAAGTTTCAGACTCTCCAAATGGCTCGAATACTTTTTTGCATATTGTGGAGCTCTCGCTTTTCAGGGTGATCCAATCGATTGGGTGAGTACACATCGATGTCATCATCAATTTGTCGATACAGAAAATGATCCGCACAGTCCTATTCAAGGATTTTGGTTTAGTTATTTCACTTGGATTTTGGATTCGAATGCTTTGACTAAAAGATATGGAAGACCAAATAATGTTGGTGACTTGGAGAAACAGTCCTTCTATAGGTTTCTTCGCAAAACTTATTTTCTTCATCAACTTGCTCTTGCAATCCTTCTTTATGCGGTTGGAGGAAAACCTTTTCTTGTATGGGGAATGTTTGTGAGGACCATAGCATTCCTACATGTGACGTTTATGTTGAATTCAATATGTCATACATTTGGAAATCAACCATGGAACACTGGAGATTTGTCTAAAAATACTTGGTGGATGTGTTTGCTTACACTTGGAGAAAGTTGGCATAATAACCATCATGCATTCGAGTATTCAGCCAGACAAGGGCTTGAATGGTGGCAGATCGACATTTGTTGGTACATTATTTGGTTTCTTCAAGTCATTGGATTAGCAACTGAGGTTAAAGTACCCTCTCAATCTCACAAGCAAAGACTACAAGCTTTGGACCAAACCAAAAGAAAAGAACTTTGA

Coding sequence (CDS)

ATGGATACAATGGTGAAGGCTGATCAAATCCCCTTTGGGGAGAGAAAATGGACAAACAGAGACAAGTATATGGCAGCTCTTTTTGTTATTATGCACTTCATTTGTATTCTTGCACCCTTCCATTTCAATTGGAATGCATTTTGGGTCGCTACTCTAAATCTTTCACATAGAAGTTTCAGACTCTCCAAATGGCTCGAATACTTTTTTGCATATTGTGGAGCTCTCGCTTTTCAGGGTGATCCAaTCGATTGGGTGAGTACACATCGATGTCATCATCAATTTGTCGATACAGAAaATGATCCGCACAGTCCTATTCAAGGATTTTGGTTTAGTTATTTCACTTGGATTTTGGATTCGAATGCTTTGACTAAAAGATATGGAAGACCAAATAATGTTGGTGACTTGGAGAAACAGTCCTTCTATAGGTTTCTTCGCAAAACTTATTTTCTTCATCAACTTGCTCTTGCAATCCTTCTTTATGCGGTTGGAGGAAAACCTTTTCTTGTATGGGGAATGTTTGTGAGGACCATAGCATTCCTACATGTGACGTTTATGTTGAATTCAATATGTCATACATTTGGAAATCAACCATGGAACACTGGAGATTTGTCTAAAAATACTTGGTGGATGTGTTTGCTTACACTTGGAGAAAGTTGGCATAATAACCATCATGCATTCGAGTATTCAGCCAGACAAGGGCTTGAATGGTGGCAGATCGACATTTGTTGGTACATTATTTGGTTTCTTCAAGTCATTGGATTAGCAACTGAGGTTAAAGTACCCTCTCAATCTCACAAGCAAAGACTACAAGCTTTGGACCAAACCAAAAGAAAAGAACTTTGA

Protein sequence

MDTMVKADQIPFGERKWTNRDKYMAALFVIMHFICILAPFHFNWNAFWVATLNLSHRSFRLSKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHSPIQGFWFSYFTWILDSNALTKRYGRPNNVGDLEKQSFYRFLRKTYFLHQLALAILLYAVGGKPFLVWGMFVRTIAFLHVTFMLNSICHTFGNQPWNTGDLSKNTWWMCLLTLGESWHNNHHAFEYSARQGLEWWQIDICWYIIWFLQVIGLATEVKVPSQSHKQRLQALDQTKRKEL*

Homology

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BLAST of Cucsa.036060.1 vs. Swiss-Prot
Match: ADS3_ARATH (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana GN=ADS3 PE=1 SV=2)

HSP 1 Score: 372.1 bits (954), Expect = 5.4e-102
Identity = 165/280 (58.93%), Postives = 206/280 (73.57%), Query Frame = 1


Query: 6   KADQIPFGERKWTNRDKYMAALFVIMHFICILAPFHFNWNAFWVA------------TL- 65
           K +++ + ER+W   D    A+ + MH + +LAPF FNW A  VA            TL 
Sbjct: 87  KKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLS 146

Query: 66  ---NLSHRSFRLSKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHSPIQGFW 125
              NLSH++F+L KWLEY FAYCGA A QG+PIDWVSTHR HHQF D++ DPHSP+ GFW
Sbjct: 147 FHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFW 206

Query: 126 FSYFTWILDSNALTKRYGRPNNVGDLEKQSFYRFLRKTYFLHQLALAILLYAVGGKPFLV 185
           FS+  W+ D+N +T+R G PNNVGDLEKQ FYRFLR TY LH LALA+ LYA+GG PF+V
Sbjct: 207 FSHMNWMFDTNTITQRCGEPNNVGDLEKQPFYRFLRTTYILHPLALAVALYAMGGFPFIV 266

Query: 186 WGMFVRTIAFLHVTFMLNSICHTFGNQPWNTGDLSKNTWWMCLLTLGESWHNNHHAFEYS 245
           WGM VR +   H+T+++NS CH +G Q WNTGDLSKN WW+  L  GE WHNNHHAFE+S
Sbjct: 267 WGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALAFGEGWHNNHHAFEFS 326

Query: 246 ARQGLEWWQIDICWYIIWFLQVIGLATEVKVPSQSHKQRL 270
           AR GLEWWQ+D+ WY++ FLQ IGLAT+VK+PS++ KQR+
Sbjct: 327 ARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEAQKQRM 366

[Close]

BLAST of Cucsa.036060.1 vs. Swiss-Prot
Match: ICO5D_LIMDO (Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii PE=1 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 1.5e-91
Identity = 152/278 (54.68%), Postives = 193/278 (69.42%), Query Frame = 1


Query: 12  FGERKWTNRDKYMAALFVIMHFICILAPFHFNWNAFWVATLNL----------------- 71
           F  RKW  RD   A   +++H +  +APF+F+W+AFW++ + L                 
Sbjct: 76  FWRRKWNPRDIQNAVTLLVLHALAAMAPFYFSWDAFWISFILLGFASGVLGITLCFHRCL 135

Query: 72  SHRSFRLSKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHSPIQGFWFSYFT 131
           +H  F+L K +EYFFAYCG+LA QGDP++WVS HR HHQFVDTE D HSP QGFWF +  
Sbjct: 136 THGGFKLPKLVEYFFAYCGSLALQGDPMEWVSNHRYHHQFVDTERDVHSPTQGFWFCHIG 195

Query: 132 WILDSNALT-KRYGRPNNVGDLEKQSFYRFLRKTYFLHQLALAILLYAVGGKPFLVWGMF 191
           W+LD +    KR GR NNV DL+KQ+FYRFL+KTY  HQLAL  LLY VGG P++VWGM 
Sbjct: 196 WVLDKDLFVEKRGGRRNNVNDLKKQAFYRFLQKTYMYHQLALIALLYYVGGFPYIVWGMG 255

Query: 192 VRTIAFLHVTFMLNSICHTFGNQPWNTGDLSKNTWWMCLLTLGESWHNNHHAFEYSARQG 251
            R +   H TF +NS+CH +G +PWNTGDLS N  ++ L   GE WHNNHHAFE SAR G
Sbjct: 256 FRLVFMFHSTFAINSVCHKWGGRPWNTGDLSTNNMFVALCAFGEGWHNNHHAFEQSARHG 315

Query: 252 LEWWQIDICWYIIWFLQVIGLATEVKVPSQSHKQRLQA 272
           LEWW+ID+ WY+I  LQ IGLAT VK+P+++ KQ+L+A
Sbjct: 316 LEWWEIDVTWYVIRTLQAIGLATNVKLPTEAQKQKLKA 353

[Close]

BLAST of Cucsa.036060.1 vs. Swiss-Prot
Match: ADS1_ARATH (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana GN=ADS1 PE=2 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 3.6e-90
Identity = 146/271 (53.87%), Postives = 189/271 (69.74%), Query Frame = 1


Query: 14  ERKWTNRDKYMAALFVIMHFICILAPFHFNWNAFWVATL---------------NLSHRS 73
           ERKW   D   A   + +HF+C+LAPF+F W A  VA +               NL+HRS
Sbjct: 31  ERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGGLGITVSYHRNLAHRS 90

Query: 74  FRLSKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHSPIQGFWFSYFTWILD 133
           F++ KWLEYFFAYCG LA QGDPIDWVSTHR HHQF D++ DPHSP +GFWFS+  W+ D
Sbjct: 91  FKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGFWFSHLLWLFD 150

Query: 134 SNALTKRYGRPNNVGDLEKQSFYRFLRKTYFLHQLALAILLYAVGGKPFLVWGMFVRTIA 193
           +  L ++ GR  NV DL++Q +Y+FL++T   H L    LLY  GG  FL WGM +    
Sbjct: 151 TGYLVEKCGRRTNVEDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAM 210

Query: 194 FLHVTFMLNSICHTFGNQPWNTGDLSKNTWWMCLLTLGESWHNNHHAFEYSARQGLEWWQ 253
             HVT ++NS+CH +G++ W T D S+N WW+ + + GESWHNNHHAFE SARQGLEWWQ
Sbjct: 211 EHHVTCLINSLCHVWGSRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQ 270

Query: 254 IDICWYIIWFLQVIGLATEVKVPSQSHKQRL 270
           IDI WYI+ FL++IGLAT+VK+PS+S ++R+
Sbjct: 271 IDISWYIVRFLEIIGLATDVKLPSESQRRRM 301

[Close]

BLAST of Cucsa.036060.1 vs. Swiss-Prot
Match: ADS32_ARATH (Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana GN=ADS3.2 PE=2 SV=3)

HSP 1 Score: 330.1 bits (845), Expect = 2.4e-89
Identity = 147/268 (54.85%), Postives = 186/268 (69.40%), Query Frame = 1


Query: 12  FGERKWTNRDKYMAALFVIMHFICILAPFHFNWNAFWV-----------ATL----NLSH 71
           F ER WT+ D     +FV  H + +LAPF+F+W AFWV            TL    NLSH
Sbjct: 86  FWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGICITLSYHRNLSH 145

Query: 72  RSFRLSKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHSPIQGFWFSYFTWI 131
           RSF L KWLEY FAY G LAFQGDPI+WVS HR HH+  +T+ DPHSP QGFWFS+  WI
Sbjct: 146 RSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGFWFSHMAWI 205

Query: 132 LDSNALTKRYGRPNNVGDLEKQSFYRFLRKTYFLHQLALAILLYAVGGKPFLVWGMFVRT 191
            D++++ +  G   NV DL +Q FYRFL++T  LH +A + L Y  GG P LVWG+ +  
Sbjct: 206 FDTSSILENCGGEENVDDLVRQPFYRFLQRTVLLHMMAYSFLFYFCGGMPLLVWGIGITI 265

Query: 192 IAFLHVTFMLNSICHTFGNQPWNTGDLSKNTWWMCLLTLGESWHNNHHAFEYSARQGLEW 251
              LH+TF++NS+CH +G + WNT D SKN WW+ +LTLGE WHNNHHAFE+SAR GLEW
Sbjct: 266 AVRLHLTFLVNSVCHIWGTRAWNTSDFSKNNWWVAILTLGEGWHNNHHAFEFSARHGLEW 325

Query: 252 WQIDICWYIIWFLQVIGLATEVKVPSQS 265
           WQ+DI W +I FL+ IGLAT VK+P+++
Sbjct: 326 WQLDITWCLIRFLEAIGLATNVKLPTET 353

[Close]

BLAST of Cucsa.036060.1 vs. Swiss-Prot
Match: ADS2_ARATH (Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana GN=ADS2 PE=2 SV=2)

HSP 1 Score: 323.9 bits (829), Expect = 1.7e-87
Identity = 143/273 (52.38%), Postives = 184/273 (67.40%), Query Frame = 1


Query: 12  FGERKWTNRDKYMAALFVIMHFICILAPFHFNWNAFWVATL---------------NLSH 71
           F +R+W   D    +    +H + +LAPF+F W+A WV  L               NL+H
Sbjct: 31  FWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGLGITVSYHRNLAH 90

Query: 72  RSFRLSKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHSPIQGFWFSYFTWI 131
           RSF++ KWLEY  AYC  LA QGDPIDWVSTHR HHQF D+E DPHSP +GFWFS+  WI
Sbjct: 91  RSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLWI 150

Query: 132 LDSNALTKRYGRPNNVGDLEKQSFYRFLRKTYFLHQLALAILLYAVGGKPFLVWGMFVRT 191
            DS  L  + GR  NV DL++Q FYRFL+KT   H L L   L+ +GG  F+ WGM V  
Sbjct: 151 YDSAYLVSKCGRRANVEDLKRQWFYRFLQKTVLFHILGLGFFLFYLGGMSFVTWGMGVGA 210

Query: 192 IAFLHVTFMLNSICHTFGNQPWNTGDLSKNTWWMCLLTLGESWHNNHHAFEYSARQGLEW 251
              +HVT ++NS+CH +G + W T D S+N WW+ + + GESWHNNHHAFE SARQGLEW
Sbjct: 211 ALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEW 270

Query: 252 WQIDICWYIIWFLQVIGLATEVKVPSQSHKQRL 270
           WQIDI WYI+ F ++IGLAT+VKVP+++ ++R+
Sbjct: 271 WQIDISWYIVRFFEIIGLATDVKVPTEAQRRRM 303

[Close]

BLAST of Cucsa.036060.1 vs. TrEMBL
Match: A0A0A0LVX7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G227460 PE=3 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 2.9e-163
Identity = 276/317 (87.07%), Postives = 278/317 (87.70%), Query Frame = 1


Query: 1   MDTMVKADQIPFGERKWTNRDKYMAALFVIMHFICILAPFHFNWNAFWVATL-------- 60
           MDTMVKADQIPFGERKWTNRDKYMAALFVIMHFICILAPFHFNWNAFWVATL        
Sbjct: 1   MDTMVKADQIPFGERKWTNRDKYMAALFVIMHFICILAPFHFNWNAFWVATLLYFFSFFG 60

Query: 61  -------NLSHRSFRLSKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHSPI 120
                  NLSHRSFRLSKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQF DT+NDPHSPI
Sbjct: 61  INISYHRNLSHRSFRLSKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFADTKNDPHSPI 120

Query: 121 QGFWFSYFTWILDSNALTKR----------------------YGRPNNVGDLEKQSFYRF 180
           QGFWFSYFTW+LDSNALTKR                      YGRPNNVGDLEKQSFYRF
Sbjct: 121 QGFWFSYFTWLLDSNALTKRVCPQYFIDHKDTDKTIFTLVLKYGRPNNVGDLEKQSFYRF 180

Query: 181 LRKTYFLHQLALAILLYAVGGKPFLVWGMFVRTIAFLHVTFMLNSICHTFGNQPWNTGDL 240
           LRKTYFLHQLALAILLYAVGG PFLVWGMFVRTIAFLHVTFMLNSICHTFGNQPWNTGDL
Sbjct: 181 LRKTYFLHQLALAILLYAVGGTPFLVWGMFVRTIAFLHVTFMLNSICHTFGNQPWNTGDL 240

Query: 241 SKNTWWMCLLTLGESWHNNHHAFEYSARQGLEWWQIDICWYIIWFLQVIGLATEVKVPSQ 281
           SKNTWWMCLLTLGESWHNNHHAFEYSARQGLEWWQIDICWYIIWFLQVIGLATEVKVPSQ
Sbjct: 241 SKNTWWMCLLTLGESWHNNHHAFEYSARQGLEWWQIDICWYIIWFLQVIGLATEVKVPSQ 300

[Close]

BLAST of Cucsa.036060.1 vs. TrEMBL
Match: A0A0A0LTL5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G227450 PE=3 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 5.4e-149
Identity = 253/319 (79.31%), Postives = 263/319 (82.45%), Query Frame = 1


Query: 1   MDTMVK-ADQIPFGERKWTNRDKYMAALFVIMHFICILAPFHFNWNAFWVATL------- 60
           MDTMVK AD I FG+RKWTNRDKYMAA F+ +HFICILAPFHFNWNAFWVA         
Sbjct: 1   MDTMVKKADLITFGQRKWTNRDKYMAAFFIFLHFICILAPFHFNWNAFWVAFALYIITGC 60

Query: 61  ---------NLSHRSFRLSKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHS 120
                    NLSHRSFRL KWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHS
Sbjct: 61  FGITISYHRNLSHRSFRLPKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHS 120

Query: 121 PIQGFWFSYFTWILDSNALTKR----------------------YGRPNNVGDLEKQSFY 180
           PIQGFWFSYF WILDSNALT+R                      YGRPNNVGDLEKQ FY
Sbjct: 121 PIQGFWFSYFIWILDSNALTRRVCPQYFIDHKDTEKTIFTVVLKYGRPNNVGDLEKQFFY 180

Query: 181 RFLRKTYFLHQLALAILLYAVGGKPFLVWGMFVRTIAFLHVTFMLNSICHTFGNQPWNTG 240
           RFLRKTYFLHQLALAILLYAVGG PFL+WG FVRTI  +HVTFM+NSICHTFGNQPWNTG
Sbjct: 181 RFLRKTYFLHQLALAILLYAVGGTPFLIWGAFVRTIGTIHVTFMVNSICHTFGNQPWNTG 240

Query: 241 DLSKNTWWMCLLTLGESWHNNHHAFEYSARQGLEWWQIDICWYIIWFLQVIGLATEVKVP 281
           DLS+NTWWMCL++ GE WHNNHHAFEYSARQGLEWWQIDI WYIIWFLQVIGLATEVKVP
Sbjct: 241 DLSRNTWWMCLISFGEGWHNNHHAFEYSARQGLEWWQIDISWYIIWFLQVIGLATEVKVP 300

[Close]

BLAST of Cucsa.036060.1 vs. TrEMBL
Match: G7JGX9_MEDTR (Fatty-acid desaturase OS=Medicago truncatula GN=MTR_4g010150 PE=3 SV=2)

HSP 1 Score: 390.2 bits (1001), Expect = 2.1e-105
Identity = 171/284 (60.21%), Postives = 209/284 (73.59%), Query Frame = 1


Query: 2   DTMVKADQIPFGERKWTNRDKYMAALFVIMHFICILAPFHFNWNAFWVATL--------- 61
           D  VK ++  F  RKW + D     + + MH +C+ APF+FNW AFWV+           
Sbjct: 99  DVEVKREKEVFFGRKWNSLDIGTFVIVLSMHLLCLFAPFNFNWPAFWVSVALYVVTGLFG 158

Query: 62  -------NLSHRSFRLSKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHSPI 121
                  NLSHRSF+L KWLEYFFAYCG LA QG+PIDWVSTHR HHQF D+E DPHSPI
Sbjct: 159 ITTSFHRNLSHRSFKLPKWLEYFFAYCGVLALQGNPIDWVSTHRYHHQFCDSEKDPHSPI 218

Query: 122 QGFWFSYFTWILDSNALTKRYGRPNNVGDLEKQSFYRFLRKTYFLHQLALAILLYAVGGK 181
           +GFWFS+ +W+ D+N++ +R G P NVGDLEKQSFYRFLR TY +H  AL  LLYA+GG 
Sbjct: 219 EGFWFSHMSWLFDTNSIAERCGEPTNVGDLEKQSFYRFLRSTYMVHPFALGALLYAIGGF 278

Query: 182 PFLVWGMFVRTIAFLHVTFMLNSICHTFGNQPWNTGDLSKNTWWMCLLTLGESWHNNHHA 241
           PFLVWGM VR +   H+T+++NS CH +G QPWNT DLS+N WW+ LL  GE WHNNHHA
Sbjct: 279 PFLVWGMGVRVVWVYHITWLVNSACHVWGKQPWNTRDLSRNNWWVALLAFGEGWHNNHHA 338

Query: 242 FEYSARQGLEWWQIDICWYIIWFLQVIGLATEVKVPSQSHKQRL 270
           FEYSAR GLEWW++D+ WY++ FLQ IGLAT+VKVPS+S KQR+
Sbjct: 339 FEYSARHGLEWWELDMTWYLVKFLQAIGLATDVKVPSESQKQRM 382

[Close]

BLAST of Cucsa.036060.1 vs. TrEMBL
Match: V7AQ17_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_010G156200g PE=3 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 1.1e-104
Identity = 170/281 (60.50%), Postives = 206/281 (73.31%), Query Frame = 1


Query: 5   VKADQIPFGERKWTNRDKYMAALFVIMHFICILAPFHFNWNAFWVATL------------ 64
           VK ++  F  RKW + D   A + ++MH +C+ APFHFNW AFWVA              
Sbjct: 136 VKRERSVFFGRKWNSLDVGTAGMVLVMHVLCLFAPFHFNWPAFWVAVALYVVTGLFGITL 195

Query: 65  ----NLSHRSFRLSKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHSPIQGF 124
               NLSHRSF+L KWLEYFFAYCGALA QG+PIDWVSTHR HHQF D+E DPHSP +GF
Sbjct: 196 SFHRNLSHRSFKLPKWLEYFFAYCGALALQGNPIDWVSTHRYHHQFCDSERDPHSPTEGF 255

Query: 125 WFSYFTWILDSNALTKRYGRPNNVGDLEKQSFYRFLRKTYFLHQLALAILLYAVGGKPFL 184
           WFS+ +W+ D+N++ +R G PNNV DLEKQ FYR LR TY +H  AL  LLYA GG PFL
Sbjct: 256 WFSHMSWLFDTNSIIERCGEPNNVVDLEKQPFYRLLRSTYLVHPFALGTLLYAAGGFPFL 315

Query: 185 VWGMFVRTIAFLHVTFMLNSICHTFGNQPWNTGDLSKNTWWMCLLTLGESWHNNHHAFEY 244
           VWGM VR +   H+T+ +NS CH +GNQ WNT DLS+N WW+ LL  GE WHNNHHAFEY
Sbjct: 316 VWGMGVRIVWVYHITWFVNSACHVWGNQAWNTRDLSRNNWWVALLAFGEGWHNNHHAFEY 375

Query: 245 SARQGLEWWQIDICWYIIWFLQVIGLATEVKVPSQSHKQRL 270
           SAR GLEWWQ+D+ WY++ FLQ +GLATEVK+P++S KQR+
Sbjct: 376 SARHGLEWWQLDMTWYVVRFLQAVGLATEVKLPTESQKQRM 416

[Close]

BLAST of Cucsa.036060.1 vs. TrEMBL
Match: A0A0S3T0J4_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.09G224600 PE=3 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 1.1e-104
Identity = 171/281 (60.85%), Postives = 205/281 (72.95%), Query Frame = 1


Query: 5   VKADQIPFGERKWTNRDKYMAALFVIMHFICILAPFHFNWNAFWVATL------------ 64
           VK ++     RKW++ D   A + + MH +C+ APFHFNW AFWVA              
Sbjct: 91  VKRERSVLFGRKWSSLDVGTAGIVLAMHVLCLFAPFHFNWPAFWVAVALYVVTGLFGITL 150

Query: 65  ----NLSHRSFRLSKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHSPIQGF 124
               NLSHRSF+L KWLEY FAYCG LA QG+PIDWVSTHR HHQF DTE DPHSP +GF
Sbjct: 151 SFHRNLSHRSFKLPKWLEYSFAYCGVLALQGNPIDWVSTHRYHHQFCDTERDPHSPTEGF 210

Query: 125 WFSYFTWILDSNALTKRYGRPNNVGDLEKQSFYRFLRKTYFLHQLALAILLYAVGGKPFL 184
           WFS+ +W+ D+N++ +R G PNNVGDLEKQ FYRFLR TY +H  AL  LLYA GG PFL
Sbjct: 211 WFSHMSWLFDTNSIVERCGEPNNVGDLEKQPFYRFLRSTYLVHPFALGALLYAAGGFPFL 270

Query: 185 VWGMFVRTIAFLHVTFMLNSICHTFGNQPWNTGDLSKNTWWMCLLTLGESWHNNHHAFEY 244
           VWGM VR +   H+T+ +NS CH +GNQ WNT DLS+N WW+ LL  GE WHNNHHAFEY
Sbjct: 271 VWGMGVRIVWVYHITWFVNSACHVWGNQAWNTRDLSRNNWWVALLAFGEGWHNNHHAFEY 330

Query: 245 SARQGLEWWQIDICWYIIWFLQVIGLATEVKVPSQSHKQRL 270
           SAR GLEWWQ+D+ WY++ FLQ IGLATEVK+PS++ KQR+
Sbjct: 331 SARHGLEWWQLDMTWYVVRFLQAIGLATEVKLPSETQKQRM 371

[Close]

BLAST of Cucsa.036060.1 vs. TAIR10
Match: AT3G15850.1 (AT3G15850.1 fatty acid desaturase 5)

HSP 1 Score: 372.1 bits (954), Expect = 3.1e-103
Identity = 165/280 (58.93%), Postives = 206/280 (73.57%), Query Frame = 1


Query: 6   KADQIPFGERKWTNRDKYMAALFVIMHFICILAPFHFNWNAFWVA------------TL- 65
           K +++ + ER+W   D    A+ + MH + +LAPF FNW A  VA            TL 
Sbjct: 87  KKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLS 146

Query: 66  ---NLSHRSFRLSKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHSPIQGFW 125
              NLSH++F+L KWLEY FAYCGA A QG+PIDWVSTHR HHQF D++ DPHSP+ GFW
Sbjct: 147 FHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFW 206

Query: 126 FSYFTWILDSNALTKRYGRPNNVGDLEKQSFYRFLRKTYFLHQLALAILLYAVGGKPFLV 185
           FS+  W+ D+N +T+R G PNNVGDLEKQ FYRFLR TY LH LALA+ LYA+GG PF+V
Sbjct: 207 FSHMNWMFDTNTITQRCGEPNNVGDLEKQPFYRFLRTTYILHPLALAVALYAMGGFPFIV 266

Query: 186 WGMFVRTIAFLHVTFMLNSICHTFGNQPWNTGDLSKNTWWMCLLTLGESWHNNHHAFEYS 245
           WGM VR +   H+T+++NS CH +G Q WNTGDLSKN WW+  L  GE WHNNHHAFE+S
Sbjct: 267 WGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALAFGEGWHNNHHAFEFS 326

Query: 246 ARQGLEWWQIDICWYIIWFLQVIGLATEVKVPSQSHKQRL 270
           AR GLEWWQ+D+ WY++ FLQ IGLAT+VK+PS++ KQR+
Sbjct: 327 ARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEAQKQRM 366

[Close]

BLAST of Cucsa.036060.1 vs. TAIR10
Match: AT1G06080.1 (AT1G06080.1 delta 9 desaturase 1)

HSP 1 Score: 332.8 bits (852), Expect = 2.1e-91
Identity = 146/271 (53.87%), Postives = 189/271 (69.74%), Query Frame = 1


Query: 14  ERKWTNRDKYMAALFVIMHFICILAPFHFNWNAFWVATL---------------NLSHRS 73
           ERKW   D   A   + +HF+C+LAPF+F W A  VA +               NL+HRS
Sbjct: 31  ERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGGLGITVSYHRNLAHRS 90

Query: 74  FRLSKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHSPIQGFWFSYFTWILD 133
           F++ KWLEYFFAYCG LA QGDPIDWVSTHR HHQF D++ DPHSP +GFWFS+  W+ D
Sbjct: 91  FKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGFWFSHLLWLFD 150

Query: 134 SNALTKRYGRPNNVGDLEKQSFYRFLRKTYFLHQLALAILLYAVGGKPFLVWGMFVRTIA 193
           +  L ++ GR  NV DL++Q +Y+FL++T   H L    LLY  GG  FL WGM +    
Sbjct: 151 TGYLVEKCGRRTNVEDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAM 210

Query: 194 FLHVTFMLNSICHTFGNQPWNTGDLSKNTWWMCLLTLGESWHNNHHAFEYSARQGLEWWQ 253
             HVT ++NS+CH +G++ W T D S+N WW+ + + GESWHNNHHAFE SARQGLEWWQ
Sbjct: 211 EHHVTCLINSLCHVWGSRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQ 270

Query: 254 IDICWYIIWFLQVIGLATEVKVPSQSHKQRL 270
           IDI WYI+ FL++IGLAT+VK+PS+S ++R+
Sbjct: 271 IDISWYIVRFLEIIGLATDVKLPSESQRRRM 301

[Close]

BLAST of Cucsa.036060.1 vs. TAIR10
Match: AT3G15870.1 (AT3G15870.1 Fatty acid desaturase family protein)

HSP 1 Score: 330.1 bits (845), Expect = 1.3e-90
Identity = 147/268 (54.85%), Postives = 186/268 (69.40%), Query Frame = 1


Query: 12  FGERKWTNRDKYMAALFVIMHFICILAPFHFNWNAFWV-----------ATL----NLSH 71
           F ER WT+ D     +FV  H + +LAPF+F+W AFWV            TL    NLSH
Sbjct: 86  FWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGICITLSYHRNLSH 145

Query: 72  RSFRLSKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHSPIQGFWFSYFTWI 131
           RSF L KWLEY FAY G LAFQGDPI+WVS HR HH+  +T+ DPHSP QGFWFS+  WI
Sbjct: 146 RSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGFWFSHMAWI 205

Query: 132 LDSNALTKRYGRPNNVGDLEKQSFYRFLRKTYFLHQLALAILLYAVGGKPFLVWGMFVRT 191
            D++++ +  G   NV DL +Q FYRFL++T  LH +A + L Y  GG P LVWG+ +  
Sbjct: 206 FDTSSILENCGGEENVDDLVRQPFYRFLQRTVLLHMMAYSFLFYFCGGMPLLVWGIGITI 265

Query: 192 IAFLHVTFMLNSICHTFGNQPWNTGDLSKNTWWMCLLTLGESWHNNHHAFEYSARQGLEW 251
              LH+TF++NS+CH +G + WNT D SKN WW+ +LTLGE WHNNHHAFE+SAR GLEW
Sbjct: 266 AVRLHLTFLVNSVCHIWGTRAWNTSDFSKNNWWVAILTLGEGWHNNHHAFEFSARHGLEW 325

Query: 252 WQIDICWYIIWFLQVIGLATEVKVPSQS 265
           WQ+DI W +I FL+ IGLAT VK+P+++
Sbjct: 326 WQLDITWCLIRFLEAIGLATNVKLPTET 353

[Close]

BLAST of Cucsa.036060.1 vs. TAIR10
Match: AT2G31360.1 (AT2G31360.1 16:0delta9 desaturase 2)

HSP 1 Score: 323.9 bits (829), Expect = 9.5e-89
Identity = 143/273 (52.38%), Postives = 184/273 (67.40%), Query Frame = 1


Query: 12  FGERKWTNRDKYMAALFVIMHFICILAPFHFNWNAFWVATL---------------NLSH 71
           F +R+W   D    +    +H + +LAPF+F W+A WV  L               NL+H
Sbjct: 31  FWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGLGITVSYHRNLAH 90

Query: 72  RSFRLSKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHSPIQGFWFSYFTWI 131
           RSF++ KWLEY  AYC  LA QGDPIDWVSTHR HHQF D+E DPHSP +GFWFS+  WI
Sbjct: 91  RSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLWI 150

Query: 132 LDSNALTKRYGRPNNVGDLEKQSFYRFLRKTYFLHQLALAILLYAVGGKPFLVWGMFVRT 191
            DS  L  + GR  NV DL++Q FYRFL+KT   H L L   L+ +GG  F+ WGM V  
Sbjct: 151 YDSAYLVSKCGRRANVEDLKRQWFYRFLQKTVLFHILGLGFFLFYLGGMSFVTWGMGVGA 210

Query: 192 IAFLHVTFMLNSICHTFGNQPWNTGDLSKNTWWMCLLTLGESWHNNHHAFEYSARQGLEW 251
              +HVT ++NS+CH +G + W T D S+N WW+ + + GESWHNNHHAFE SARQGLEW
Sbjct: 211 ALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEW 270

Query: 252 WQIDICWYIIWFLQVIGLATEVKVPSQSHKQRL 270
           WQIDI WYI+ F ++IGLAT+VKVP+++ ++R+
Sbjct: 271 WQIDISWYIVRFFEIIGLATDVKVPTEAQRRRM 303

[Close]

BLAST of Cucsa.036060.1 vs. TAIR10
Match: AT1G06100.1 (AT1G06100.1 Fatty acid desaturase family protein)

HSP 1 Score: 317.0 bits (811), Expect = 1.2e-86
Identity = 142/280 (50.71%), Postives = 189/280 (67.50%), Query Frame = 1


Query: 5   VKADQIPFGERKWTNRDKYMAALFVIMHFICILAPFHFNWNAFWVATL------------ 64
           V+ ++  +  RKWT  D   A+    +H +C+LAPF++ W AF    +            
Sbjct: 16  VRKEKRAYVLRKWTQFDVGRASTVGTVHLLCLLAPFNYKWEAFRFGIILAILTNLCITFS 75

Query: 65  ---NLSHRSFRLSKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHSPIQGFW 124
              NL+HRSF+L KWLEY FAY   LA QGDP+DWVS HR HHQF D++ DPHSPI+GFW
Sbjct: 76  YHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDWVSIHRFHHQFTDSDRDPHSPIEGFW 135

Query: 125 FSYFTWILDSNALTKRYGRPNNVGDLEKQSFYRFLRKTYFLHQLALAILLYAVGGKPFLV 184
           FS+  WI D++ + ++ GR NNV DL++Q FYRFL+KT  LH LA   L+Y  GG P+L 
Sbjct: 136 FSHVLWIFDTDYIREKCGRRNNVMDLKQQWFYRFLKKTLVLHILAFWTLIYLWGGLPYLT 195

Query: 185 WGMFVRTIAFLHVTFMLNSICHTFGNQPWNTGDLSKNTWWMCLLTLGESWHNNHHAFEYS 244
           W +    +   H T+++NS CH  G+Q W T D S+N WW+ LLT+GESWHNNHHAFE S
Sbjct: 196 WTVGFGGVIGYHGTWLVNSACHICGSQAWQTNDTSRNVWWLALLTMGESWHNNHHAFETS 255

Query: 245 ARQGLEWWQIDICWYIIWFLQVIGLATEVKVPSQSHKQRL 270
           AR GLEW+Q+DI WY+IWF Q +GLAT VK+P+ + K+++
Sbjct: 256 ARHGLEWYQLDITWYLIWFFQALGLATNVKLPTDAQKRKM 295

[Close]

BLAST of Cucsa.036060.1 vs. NCBI nr
Match: gi|778659951|ref|XP_011655378.1| (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 595.1 bits (1533), Expect = 6.3e-167
Identity = 276/295 (93.56%), Postives = 278/295 (94.24%), Query Frame = 1


Query: 1   MDTMVKADQIPFGERKWTNRDKYMAALFVIMHFICILAPFHFNWNAFWVATL-------- 60
           MDTMVKADQIPFGERKWTNRDKYMAALFVIMHFICILAPFHFNWNAFWVATL        
Sbjct: 1   MDTMVKADQIPFGERKWTNRDKYMAALFVIMHFICILAPFHFNWNAFWVATLLYFFSFFG 60

Query: 61  -------NLSHRSFRLSKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHSPI 120
                  NLSHRSFRLSKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQF DT+NDPHSPI
Sbjct: 61  INISYHRNLSHRSFRLSKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFADTKNDPHSPI 120

Query: 121 QGFWFSYFTWILDSNALTKRYGRPNNVGDLEKQSFYRFLRKTYFLHQLALAILLYAVGGK 180
           QGFWFSYFTW+LDSNALTKRYGRPNNVGDLEKQSFYRFLRKTYFLHQLALAILLYAVGG 
Sbjct: 121 QGFWFSYFTWLLDSNALTKRYGRPNNVGDLEKQSFYRFLRKTYFLHQLALAILLYAVGGT 180

Query: 181 PFLVWGMFVRTIAFLHVTFMLNSICHTFGNQPWNTGDLSKNTWWMCLLTLGESWHNNHHA 240
           PFLVWGMFVRTIAFLHVTFMLNSICHTFGNQPWNTGDLSKNTWWMCLLTLGESWHNNHHA
Sbjct: 181 PFLVWGMFVRTIAFLHVTFMLNSICHTFGNQPWNTGDLSKNTWWMCLLTLGESWHNNHHA 240

Query: 241 FEYSARQGLEWWQIDICWYIIWFLQVIGLATEVKVPSQSHKQRLQALDQTKRKEL 281
           FEYSARQGLEWWQIDICWYIIWFLQVIGLATEVKVPSQSHKQRLQALDQTKRKEL
Sbjct: 241 FEYSARQGLEWWQIDICWYIIWFLQVIGLATEVKVPSQSHKQRLQALDQTKRKEL 295

[Close]

BLAST of Cucsa.036060.1 vs. NCBI nr
Match: gi|700210023|gb|KGN65119.1| (hypothetical protein Csa_1G227460 [Cucumis sativus])

HSP 1 Score: 582.4 bits (1500), Expect = 4.2e-163
Identity = 276/317 (87.07%), Postives = 278/317 (87.70%), Query Frame = 1


Query: 1   MDTMVKADQIPFGERKWTNRDKYMAALFVIMHFICILAPFHFNWNAFWVATL-------- 60
           MDTMVKADQIPFGERKWTNRDKYMAALFVIMHFICILAPFHFNWNAFWVATL        
Sbjct: 1   MDTMVKADQIPFGERKWTNRDKYMAALFVIMHFICILAPFHFNWNAFWVATLLYFFSFFG 60

Query: 61  -------NLSHRSFRLSKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHSPI 120
                  NLSHRSFRLSKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQF DT+NDPHSPI
Sbjct: 61  INISYHRNLSHRSFRLSKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFADTKNDPHSPI 120

Query: 121 QGFWFSYFTWILDSNALTKR----------------------YGRPNNVGDLEKQSFYRF 180
           QGFWFSYFTW+LDSNALTKR                      YGRPNNVGDLEKQSFYRF
Sbjct: 121 QGFWFSYFTWLLDSNALTKRVCPQYFIDHKDTDKTIFTLVLKYGRPNNVGDLEKQSFYRF 180

Query: 181 LRKTYFLHQLALAILLYAVGGKPFLVWGMFVRTIAFLHVTFMLNSICHTFGNQPWNTGDL 240
           LRKTYFLHQLALAILLYAVGG PFLVWGMFVRTIAFLHVTFMLNSICHTFGNQPWNTGDL
Sbjct: 181 LRKTYFLHQLALAILLYAVGGTPFLVWGMFVRTIAFLHVTFMLNSICHTFGNQPWNTGDL 240

Query: 241 SKNTWWMCLLTLGESWHNNHHAFEYSARQGLEWWQIDICWYIIWFLQVIGLATEVKVPSQ 281
           SKNTWWMCLLTLGESWHNNHHAFEYSARQGLEWWQIDICWYIIWFLQVIGLATEVKVPSQ
Sbjct: 241 SKNTWWMCLLTLGESWHNNHHAFEYSARQGLEWWQIDICWYIIWFLQVIGLATEVKVPSQ 300

[Close]

BLAST of Cucsa.036060.1 vs. NCBI nr
Match: gi|778665038|ref|XP_011648471.1| (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 538.1 bits (1385), Expect = 9.1e-150
Identity = 243/296 (82.09%), Postives = 259/296 (87.50%), Query Frame = 1


Query: 1   MDTMVKADQIPFGERKWTNRDKYMAALFVIMHFICILAPFHFNWNAFWVATL-------- 60
           MD + KAD I FGERKWTNRDKYMAALF+ MH ICILAPFHFNWNAFW+A          
Sbjct: 1   MDALEKADLISFGERKWTNRDKYMAALFITMHLICILAPFHFNWNAFWIAVAFYVITGCF 60

Query: 61  --------NLSHRSFRLSKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHSP 120
                   NLSHRSFRL KWLEYFFAYCG LAFQGDPIDWVSTHRCHHQF DT+NDPHSP
Sbjct: 61  GINISYHRNLSHRSFRLPKWLEYFFAYCGTLAFQGDPIDWVSTHRCHHQFADTKNDPHSP 120

Query: 121 IQGFWFSYFTWILDSNALTKRYGRPNNVGDLEKQSFYRFLRKTYFLHQLALAILLYAVGG 180
           IQGFWFSYFTW+LDSNALTKRYGRP+NVGDLEKQSFYRFLRKTYFLH L+LA+LLY VGG
Sbjct: 121 IQGFWFSYFTWLLDSNALTKRYGRPHNVGDLEKQSFYRFLRKTYFLHHLSLAVLLYGVGG 180

Query: 181 KPFLVWGMFVRTIAFLHVTFMLNSICHTFGNQPWNTGDLSKNTWWMCLLTLGESWHNNHH 240
            PFL+WGMFVRT+AFLH++F++ SI HTFGNQPWNTGDLS N WWMCL++ GE WHNNHH
Sbjct: 181 TPFLIWGMFVRTLAFLHMSFIVASIGHTFGNQPWNTGDLSTNNWWMCLISFGEGWHNNHH 240

Query: 241 AFEYSARQGLEWWQIDICWYIIWFLQVIGLATEVKVPSQSHKQRLQALDQTKRKEL 281
           AFEYSARQGLEWWQIDICWYIIWFLQVIGLATEVKVPSQSHKQRLQALDQ KRKEL
Sbjct: 241 AFEYSARQGLEWWQIDICWYIIWFLQVIGLATEVKVPSQSHKQRLQALDQKKRKEL 296

[Close]

BLAST of Cucsa.036060.1 vs. NCBI nr
Match: gi|700210022|gb|KGN65118.1| (hypothetical protein Csa_1G227450 [Cucumis sativus])

HSP 1 Score: 535.0 bits (1377), Expect = 7.7e-149
Identity = 253/319 (79.31%), Postives = 263/319 (82.45%), Query Frame = 1


Query: 1   MDTMVK-ADQIPFGERKWTNRDKYMAALFVIMHFICILAPFHFNWNAFWVATL------- 60
           MDTMVK AD I FG+RKWTNRDKYMAA F+ +HFICILAPFHFNWNAFWVA         
Sbjct: 1   MDTMVKKADLITFGQRKWTNRDKYMAAFFIFLHFICILAPFHFNWNAFWVAFALYIITGC 60

Query: 61  ---------NLSHRSFRLSKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHS 120
                    NLSHRSFRL KWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHS
Sbjct: 61  FGITISYHRNLSHRSFRLPKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHS 120

Query: 121 PIQGFWFSYFTWILDSNALTKR----------------------YGRPNNVGDLEKQSFY 180
           PIQGFWFSYF WILDSNALT+R                      YGRPNNVGDLEKQ FY
Sbjct: 121 PIQGFWFSYFIWILDSNALTRRVCPQYFIDHKDTEKTIFTVVLKYGRPNNVGDLEKQFFY 180

Query: 181 RFLRKTYFLHQLALAILLYAVGGKPFLVWGMFVRTIAFLHVTFMLNSICHTFGNQPWNTG 240
           RFLRKTYFLHQLALAILLYAVGG PFL+WG FVRTI  +HVTFM+NSICHTFGNQPWNTG
Sbjct: 181 RFLRKTYFLHQLALAILLYAVGGTPFLIWGAFVRTIGTIHVTFMVNSICHTFGNQPWNTG 240

Query: 241 DLSKNTWWMCLLTLGESWHNNHHAFEYSARQGLEWWQIDICWYIIWFLQVIGLATEVKVP 281
           DLS+NTWWMCL++ GE WHNNHHAFEYSARQGLEWWQIDI WYIIWFLQVIGLATEVKVP
Sbjct: 241 DLSRNTWWMCLISFGEGWHNNHHAFEYSARQGLEWWQIDISWYIIWFLQVIGLATEVKVP 300

[Close]

BLAST of Cucsa.036060.1 vs. NCBI nr
Match: gi|659086462|ref|XP_008443947.1| (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like isoform X1 [Cucumis melo])

HSP 1 Score: 533.5 bits (1373), Expect = 2.2e-148
Identity = 251/318 (78.93%), Postives = 262/318 (82.39%), Query Frame = 1


Query: 1   MDTMVKADQIPFGERKWTNRDKYMAALFVIMHFICILAPFHFNWNAFWVATL-------- 60
           M+TMVKAD IPFGERKWT+RDKYMA L +IMH ICI APFHFNWNAFWVA          
Sbjct: 1   METMVKADLIPFGERKWTDRDKYMAGLLIIMHLICITAPFHFNWNAFWVAAALHIIIDIC 60

Query: 61  --------NLSHRSFRLSKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHSP 120
                   NLSHRSF+L KWLEYF AYCG LAFQGDPIDWVSTHRCHHQFVDTENDPHSP
Sbjct: 61  GINISYHRNLSHRSFKLPKWLEYFLAYCGTLAFQGDPIDWVSTHRCHHQFVDTENDPHSP 120

Query: 121 IQGFWFSYFTWILDSNALTKR----------------------YGRPNNVGDLEKQSFYR 180
           IQGFWFSYFTW+LDSNALT+R                      YGRPNNVGDLEKQSFYR
Sbjct: 121 IQGFWFSYFTWLLDSNALTRRVCPQYFIDHKDTEKTIFTLVLKYGRPNNVGDLEKQSFYR 180

Query: 181 FLRKTYFLHQLALAILLYAVGGKPFLVWGMFVRTIAFLHVTFMLNSICHTFGNQPWNTGD 240
           FLRKTYFLHQL LAILL+AVGG PFL+WG FVRTI  LHVTFM+NSICHTFGNQPWNTGD
Sbjct: 181 FLRKTYFLHQLPLAILLHAVGGTPFLIWGTFVRTIVSLHVTFMVNSICHTFGNQPWNTGD 240

Query: 241 LSKNTWWMCLLTLGESWHNNHHAFEYSARQGLEWWQIDICWYIIWFLQVIGLATEVKVPS 281
           LS+NTWW+CLLT GESWHNNHHAFEYSAR GLEWWQIDICWYII FLQVIGLATEVKVPS
Sbjct: 241 LSRNTWWICLLTHGESWHNNHHAFEYSARHGLEWWQIDICWYIIRFLQVIGLATEVKVPS 300

The following BLAST results are available for this feature:

BLAST of Cucsa.036060.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ADS3_ARATH	5.4e-102	58.93	Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab...	[more]
ICO5D_LIMDO	1.5e-91	54.68	Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii PE=1 SV=1	[more]
ADS1_ARATH	3.6e-90	53.87	Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana GN=ADS1 PE=2 SV=1	[more]
ADS32_ARATH	2.4e-89	54.85	Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana GN=ADS3....	[more]
ADS2_ARATH	1.7e-87	52.38	Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana GN=ADS2 PE=2 SV=2	[more]

BLAST of Cucsa.036060.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LVX7_CUCSA	2.9e-163	87.07	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G227460 PE=3 SV=1	[more]
A0A0A0LTL5_CUCSA	5.4e-149	79.31	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G227450 PE=3 SV=1	[more]
G7JGX9_MEDTR	2.1e-105	60.21	Fatty-acid desaturase OS=Medicago truncatula GN=MTR_4g010150 PE=3 SV=2	[more]
V7AQ17_PHAVU	1.1e-104	60.50	Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_010G156200g PE=3 SV=1	[more]
A0A0S3T0J4_PHAAN	1.1e-104	60.85	Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.09G224600 PE=...	[more]

BLAST of Cucsa.036060.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G15850.1	3.1e-103	58.93	fatty acid desaturase 5	[more]
AT1G06080.1	2.1e-91	53.87	delta 9 desaturase 1	[more]
AT3G15870.1	1.3e-90	54.85	Fatty acid desaturase family protein	[more]
AT2G31360.1	9.5e-89	52.38	16:0delta9 desaturase 2	[more]
AT1G06100.1	1.2e-86	50.71	Fatty acid desaturase family protein	[more]

BLAST of Cucsa.036060.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778659951\|ref\|XP_011655378.1\|	6.3e-167	93.56	PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas...	[more]
gi\|700210023\|gb\|KGN65119.1\|	4.2e-163	87.07	hypothetical protein Csa_1G227460 [Cucumis sativus]	[more]
gi\|778665038\|ref\|XP_011648471.1\|	9.1e-150	82.09	PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas...	[more]
gi\|700210022\|gb\|KGN65118.1\|	7.7e-149	79.31	hypothetical protein Csa_1G227450 [Cucumis sativus]	[more]
gi\|659086462\|ref\|XP_008443947.1\|	2.2e-148	78.93	PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR005804	FA_desaturase_dom
IPR015876	Acyl-CoA_DS

Vocabulary: **Biological Process**
Term	Definition
GO:0006629	lipid metabolic process
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016717	oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0006633	fatty acid biosynthetic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016717	oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsa.036060	Cucsa.036060	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsa.036060.1	Cucsa.036060.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.036060.1.CDS.1	Cucsa.036060.1.CDS.1	CDS
Cucsa.036060.1.CDS.2	Cucsa.036060.1.CDS.2	CDS
Cucsa.036060.1.CDS.3	Cucsa.036060.1.CDS.3	CDS
Cucsa.036060.1.CDS.4	Cucsa.036060.1.CDS.4	CDS
Cucsa.036060.1.CDS.5	Cucsa.036060.1.CDS.5	CDS
Cucsa.036060.1.CDS.6	Cucsa.036060.1.CDS.6	CDS

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005804	Fatty acid desaturase domain	PFAM	PF00487	FA_desaturase	coord: 54..245 score: 6.4
IPR015876	Fatty acid desaturase, type 1, core	PRINTS	PR00075	FACDDSATRASE	coord: 216..230 score: 1.9E-20coord: 51..71 score: 1.9E-20coord: 173..194 score: 1.9E-20coord: 88..117 score: 1.9
None	No IPR available	PANTHER	PTHR11351	ACYL-COA DESATURASE	coord: 2..269 score: 1.4E
None	No IPR available	PANTHER	PTHR11351:SF2	DELTA-9 ACYL-LIPID DESATURASE 1-RELATED	coord: 2..269 score: 1.4E