Cucsa.017030.1 (mRNA) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.017030.1
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	ralf-like 9
Location	scaffold00233 : 191414 .. 191719 (-)
Sequence length	306

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATCATGTCAAAGAGAATGACACTTGTTTTTATTGGGATTCTCTTGGTTAGCTTCGACATGATTATGATCACAAATGCAAAAAAATTCATTGATTACGGTTCTATTGTTGCAGGAGATGTTAGCCCTGGATGTAGCCCAACGCACCCTGAACTTTGTAGAGTAAAGAGCGCAAATCCATACCAAAGAGGTTGCAATCGAATCGATCGTTGTAGAGAGGGAAATGATATTATTGACGCTGAAGAAGAACATATTGAAGGGGATGCATCGATTAGTCCTTCAATAAGCCCTAATATAGAGAATTAA

mRNA sequence

ATGATCATGTCAAAGAGAATGACACTTGTTTTTATTGGGATTCTCTTGGTTAGCTTCGACATGATTATGATCACAAATGCAAAAAAATTCATTGATTACGGTTCTATTGTTGCAGGAGATGTTAGCCCTGGATGTAGCCCAACGCACCCTGAACTTTGTAGAGTAAAGAGCGCAAATCCATACCAAAGAGGTTGCAATCGAATCGATCGTTGTAGAGAGGGAAATGATATTATTGACGCTGAAGAAGAACATATTGAAGGGGATGCATCGATTAGTCCTTCAATAAGCCCTAATATAGAGAATTAA

Coding sequence (CDS)

ATGATCATGTCAAAGAGAATGACACTTGTTTTTATTGGGATTCTCTTGGTTAGCTTCGACATGATTATGATCACAAATGCAAAAAAATTCATTGATTACGGTTCTATTGTTGCAGGAGATGTTAGCCCTGGATGTAGCCCAACGCACCCTGAACTTTGTAGAGTAAAGAGCGCAAATCCATACCAAAGAGGTTGCAATCGAATCGATCGTTGTAGAGAGGGAAATGATATTATTGACGCTGAAGAAGAACATATTGAAGGGGATGCATCGATTAGTCCTTCAATAAGCCCTAATATAGAGAATTAA

Protein sequence

MIMSKRMTLVFIGILLVSFDMIMITNAKKFIDYGSIVAGDVSPGCSPTHPELCRVKSANPYQRGCNRIDRCREGNDIIDAEEEHIEGDASISPSISPNIEN*

Homology

[Close]

BLAST of Cucsa.017030.1 vs. Swiss-Prot
Match: RLF9_ARATH (Protein RALF-like 9 OS=Arabidopsis thaliana GN=RALFL9 PE=3 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 9.7e-09
Identity = 32/76 (42.11%), Postives = 45/76 (59.21%), Query Frame = 1


Query: 1  MIMSKRMTLVFIGILLVSFDMIMITN--AKKFIDYGSIVAGDVSPGCSPTHPELCRVKSA 60
          M MSK + ++ + + LV F  +  T   A ++I Y +I  GD +  C   HP  C+ K A
Sbjct: 1  MGMSKSIKVI-LSLALVVFLALAATKVEATRYITYPAIDRGDHAVHCDKAHPNTCKKKEA 60

Query: 61 NPYQRGCNRIDRCREG 75
          NPYQRGC +I+RCR G
Sbjct: 61 NPYQRGCEKINRCRGG 75

[Close]

BLAST of Cucsa.017030.1 vs. Swiss-Prot
Match: RLF15_ARATH (Protein RALF-like 15 OS=Arabidopsis thaliana GN=RALFL15 PE=3 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 2.6e-06
Identity = 27/74 (36.49%), Postives = 42/74 (56.76%), Query Frame = 1


Query: 1  MIMSKRMTLVFIGILLVSFDMIMITN--AKKFIDYGSIVAGDVSPGCSPTHPELCRVKSA 60
          M MSK + ++ + + L+ F  +  T   A ++I Y  +  GD +  C   HP  C+ + A
Sbjct: 1  MGMSKSIKVI-VSLALILFLALAATKVEATRYISYRGMNHGDHAIHCDKAHPNTCKKQVA 60

Query: 61 NPYQRGCNRIDRCR 73
          NPY+RGC  I+RCR
Sbjct: 61 NPYRRGCGTIERCR 73

[Close]

BLAST of Cucsa.017030.1 vs. TrEMBL
Match: A0A0A0L8B1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G426350 PE=4 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 1.2e-50
Identity = 100/101 (99.01%), Postives = 101/101 (100.00%), Query Frame = 1


Query: 1   MIMSKRMTLVFIGILLVSFDMIMITNAKKFIDYGSIVAGDVSPGCSPTHPELCRVKSANP 60
           MIMSKRMTLVFIGILLVSFDMIMITNA+KFIDYGSIVAGDVSPGCSPTHPELCRVKSANP
Sbjct: 1   MIMSKRMTLVFIGILLVSFDMIMITNARKFIDYGSIVAGDVSPGCSPTHPELCRVKSANP 60

Query: 61  YQRGCNRIDRCREGNDIIDAEEEHIEGDASISPSISPNIEN 102
           YQRGCNRIDRCREGNDIIDAEEEHIEGDASISPSISPNIEN
Sbjct: 61  YQRGCNRIDRCREGNDIIDAEEEHIEGDASISPSISPNIEN 101

[Close]

BLAST of Cucsa.017030.1 vs. TrEMBL
Match: V4UF46_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10018043mg PE=4 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.2e-08
Identity = 35/75 (46.67%), Postives = 50/75 (66.67%), Query Frame = 1


Query: 1  MIMSKRMTLVFIGILLVSFDMIMITN---AKKFIDYGSIVAGDVSPGCSPTHPELCRVKS 60
          M+MSKRM +VF  I++++  ++M+       K I YG+I AG  +PGCSP HPELC+++ 
Sbjct: 1  MMMSKRM-MVFGVIMVLTASIVMVVKDGAEAKEIGYGAIGAGG-NPGCSPVHPELCKMEP 60

Query: 61 ANPYQRGCNRIDRCR 73
           NPY RGC    +CR
Sbjct: 61 GNPYHRGCENEAQCR 73

[Close]

BLAST of Cucsa.017030.1 vs. TrEMBL
Match: A0A067F0E4_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g041712mg PE=4 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.2e-08
Identity = 35/75 (46.67%), Postives = 50/75 (66.67%), Query Frame = 1


Query: 1  MIMSKRMTLVFIGILLVSFDMIMITN---AKKFIDYGSIVAGDVSPGCSPTHPELCRVKS 60
          M+MSKRM +VF  I++++  ++M+       K I YG+I AG  +PGCSP HPELC+++ 
Sbjct: 1  MMMSKRM-MVFGVIMVLTASIVMVVKDGAEAKEIGYGAIGAGG-NPGCSPVHPELCKMEP 60

Query: 61 ANPYQRGCNRIDRCR 73
           NPY RGC    +CR
Sbjct: 61 GNPYHRGCENEAQCR 73

[Close]

BLAST of Cucsa.017030.1 vs. TrEMBL
Match: R0I9N4_9BRAS (Uncharacterized protein OS=Capsella rubella GN=CARUB_v10022412mg PE=4 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 1.4e-06
Identity = 30/73 (41.10%), Postives = 40/73 (54.79%), Query Frame = 1


Query: 1  MIMSKRMTLVFIGILLVSFDMIMI-TNAKKFIDYGSIVAGDVSPGCSPTHPELCRVKSAN 60
          M MSK +  +   +L+V   +    T A KFIDYG +  GD S  C    PE C+ +  N
Sbjct: 1  MGMSKSVKFILSLVLVVFLALAATKTEASKFIDYGDLKRGDHSRNCDRARPESCQKEQVN 60

Query: 61 PYQRGCNRIDRCR 73
          PY+RGC +  RCR
Sbjct: 61 PYRRGCEKSQRCR 73

[Close]

BLAST of Cucsa.017030.1 vs. TrEMBL
Match: D7KVJ4_ARALL (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_893358 PE=4 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 5.4e-06
Identity = 30/76 (39.47%), Postives = 43/76 (56.58%), Query Frame = 1


Query: 1  MIMSKRMTLVFIGILLVSFDMIMI-TNAKKFIDYGSIVAGDVSPGCSPTHPELCRVKSAN 60
          M MSK + ++    L+V   +  I   A ++I Y SI  GD +  C   HP  C+ K AN
Sbjct: 1  MGMSKSIKVILSLALVVFLALASIKVEATRYITYPSINHGDHATHCDKAHPNTCKKKEAN 60

Query: 61 PYQRGCNRIDRCREGN 76
          PY+RGC  ++RCR G+
Sbjct: 61 PYKRGCEILERCRGGS 76

[Close]

BLAST of Cucsa.017030.1 vs. TAIR10
Match: AT1G61566.1 (AT1G61566.1 ralf-like 9)

HSP 1 Score: 60.8 bits (146), Expect = 5.5e-10
Identity = 32/76 (42.11%), Postives = 45/76 (59.21%), Query Frame = 1


Query: 1  MIMSKRMTLVFIGILLVSFDMIMITN--AKKFIDYGSIVAGDVSPGCSPTHPELCRVKSA 60
          M MSK + ++ + + LV F  +  T   A ++I Y +I  GD +  C   HP  C+ K A
Sbjct: 1  MGMSKSIKVI-LSLALVVFLALAATKVEATRYITYPAIDRGDHAVHCDKAHPNTCKKKEA 60

Query: 61 NPYQRGCNRIDRCREG 75
          NPYQRGC +I+RCR G
Sbjct: 61 NPYQRGCEKINRCRGG 75

[Close]

BLAST of Cucsa.017030.1 vs. TAIR10
Match: AT2G22055.1 (AT2G22055.1 RALF-like 15)

HSP 1 Score: 52.8 bits (125), Expect = 1.5e-07
Identity = 27/74 (36.49%), Postives = 42/74 (56.76%), Query Frame = 1


Query: 1  MIMSKRMTLVFIGILLVSFDMIMITN--AKKFIDYGSIVAGDVSPGCSPTHPELCRVKSA 60
          M MSK + ++ + + L+ F  +  T   A ++I Y  +  GD +  C   HP  C+ + A
Sbjct: 1  MGMSKSIKVI-VSLALILFLALAATKVEATRYISYRGMNHGDHAIHCDKAHPNTCKKQVA 60

Query: 61 NPYQRGCNRIDRCR 73
          NPY+RGC  I+RCR
Sbjct: 61 NPYRRGCGTIERCR 73

[Close]

BLAST of Cucsa.017030.1 vs. TAIR10
Match: AT1G61563.1 (AT1G61563.1 ralf-like 8)

HSP 1 Score: 48.5 bits (114), Expect = 2.8e-06
Identity = 26/74 (35.14%), Postives = 41/74 (55.41%), Query Frame = 1


Query: 1  MIMSKRMTLVFIGILLVSFDMIMITNAK---KFIDYGSIVAGDVSPGCSPTHPELCRVKS 60
          M MSK + ++ + + LV F  +  T  +   ++I Y +I  GD +  C   HP  C+ K 
Sbjct: 1  MGMSKSIKVI-LSLALVVFLALAGTKVEASVRYITYPAIDRGDHAVHCDKAHPNTCKKKQ 60

Query: 61 ANPYQRGCNRIDRC 72
          ANPY+RGC  ++ C
Sbjct: 61 ANPYRRGCGVLEGC 73

[Close]

BLAST of Cucsa.017030.1 vs. NCBI nr
Match: gi|700202865|gb|KGN57998.1| (hypothetical protein Csa_3G426350 [Cucumis sativus])

HSP 1 Score: 206.8 bits (525), Expect = 1.7e-50
Identity = 100/101 (99.01%), Postives = 101/101 (100.00%), Query Frame = 1


Query: 1   MIMSKRMTLVFIGILLVSFDMIMITNAKKFIDYGSIVAGDVSPGCSPTHPELCRVKSANP 60
           MIMSKRMTLVFIGILLVSFDMIMITNA+KFIDYGSIVAGDVSPGCSPTHPELCRVKSANP
Sbjct: 1   MIMSKRMTLVFIGILLVSFDMIMITNARKFIDYGSIVAGDVSPGCSPTHPELCRVKSANP 60

Query: 61  YQRGCNRIDRCREGNDIIDAEEEHIEGDASISPSISPNIEN 102
           YQRGCNRIDRCREGNDIIDAEEEHIEGDASISPSISPNIEN
Sbjct: 61  YQRGCNRIDRCREGNDIIDAEEEHIEGDASISPSISPNIEN 101

[Close]

BLAST of Cucsa.017030.1 vs. NCBI nr
Match: gi|567910725|ref|XP_006447676.1| (hypothetical protein CICLE_v10018043mg [Citrus clementina])

HSP 1 Score: 67.4 bits (163), Expect = 1.7e-08
Identity = 35/75 (46.67%), Postives = 50/75 (66.67%), Query Frame = 1


Query: 1  MIMSKRMTLVFIGILLVSFDMIMITN---AKKFIDYGSIVAGDVSPGCSPTHPELCRVKS 60
          M+MSKRM +VF  I++++  ++M+       K I YG+I AG  +PGCSP HPELC+++ 
Sbjct: 1  MMMSKRM-MVFGVIMVLTASIVMVVKDGAEAKEIGYGAIGAGG-NPGCSPVHPELCKMEP 60

Query: 61 ANPYQRGCNRIDRCR 73
           NPY RGC    +CR
Sbjct: 61 GNPYHRGCENEAQCR 73

[Close]

BLAST of Cucsa.017030.1 vs. NCBI nr
Match: gi|18407238|ref|NP_564779.1| (protein RALF-like 9 [Arabidopsis thaliana])

HSP 1 Score: 60.8 bits (146), Expect = 1.5e-06
Identity = 32/76 (42.11%), Postives = 45/76 (59.21%), Query Frame = 1


Query: 1  MIMSKRMTLVFIGILLVSFDMIMITN--AKKFIDYGSIVAGDVSPGCSPTHPELCRVKSA 60
          M MSK + ++ + + LV F  +  T   A ++I Y +I  GD +  C   HP  C+ K A
Sbjct: 1  MGMSKSIKVI-LSLALVVFLALAATKVEATRYITYPAIDRGDHAVHCDKAHPNTCKKKEA 60

Query: 61 NPYQRGCNRIDRCREG 75
          NPYQRGC +I+RCR G
Sbjct: 61 NPYQRGCEKINRCRGG 75

[Close]

BLAST of Cucsa.017030.1 vs. NCBI nr
Match: gi|565488585|ref|XP_006301932.1| (hypothetical protein CARUB_v10022412mg [Capsella rubella])

HSP 1 Score: 60.5 bits (145), Expect = 2.0e-06
Identity = 30/73 (41.10%), Postives = 40/73 (54.79%), Query Frame = 1


Query: 1  MIMSKRMTLVFIGILLVSFDMIMI-TNAKKFIDYGSIVAGDVSPGCSPTHPELCRVKSAN 60
          M MSK +  +   +L+V   +    T A KFIDYG +  GD S  C    PE C+ +  N
Sbjct: 1  MGMSKSVKFILSLVLVVFLALAATKTEASKFIDYGDLKRGDHSRNCDRARPESCQKEQVN 60

Query: 61 PYQRGCNRIDRCR 73
          PY+RGC +  RCR
Sbjct: 61 PYRRGCEKSQRCR 73

[Close]

BLAST of Cucsa.017030.1 vs. NCBI nr
Match: gi|21592626|gb|AAM64575.1| (unknown [Arabidopsis thaliana])

HSP 1 Score: 59.7 bits (143), Expect = 3.4e-06
Identity = 32/76 (42.11%), Postives = 45/76 (59.21%), Query Frame = 1


Query: 1  MIMSKRMTLVFIGILLVSFDMIMITN--AKKFIDYGSIVAGDVSPGCSPTHPELCRVKSA 60
          M MSK + ++ + + LV F  +  T   A ++I Y +I  GD +  C   HP  C+ K A
Sbjct: 1  MGMSKSIKVI-LSLALVVFLALAGTKVEATRYITYPAIDRGDHAVHCDKAHPNTCKKKQA 60

Query: 61 NPYQRGCNRIDRCREG 75
          NPYQRGC +I+RCR G
Sbjct: 61 NPYQRGCEKINRCRGG 75

The following BLAST results are available for this feature:

BLAST of Cucsa.017030.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RLF9_ARATH	9.7e-09	42.11	Protein RALF-like 9 OS=Arabidopsis thaliana GN=RALFL9 PE=3 SV=1	[more]
RLF15_ARATH	2.6e-06	36.49	Protein RALF-like 15 OS=Arabidopsis thaliana GN=RALFL15 PE=3 SV=1	[more]

BLAST of Cucsa.017030.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L8B1_CUCSA	1.2e-50	99.01	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G426350 PE=4 SV=1	[more]
V4UF46_9ROSI	1.2e-08	46.67	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10018043mg PE=4 SV=1	[more]
A0A067F0E4_CITSI	1.2e-08	46.67	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g041712mg PE=4 SV=1	[more]
R0I9N4_9BRAS	1.4e-06	41.10	Uncharacterized protein OS=Capsella rubella GN=CARUB_v10022412mg PE=4 SV=1	[more]
D7KVJ4_ARALL	5.4e-06	39.47	Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRA...	[more]

BLAST of Cucsa.017030.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G61566.1	5.5e-10	42.11	ralf-like 9	[more]
AT2G22055.1	1.5e-07	36.49	RALF-like 15	[more]
AT1G61563.1	2.8e-06	35.14	ralf-like 8	[more]

BLAST of Cucsa.017030.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700202865\|gb\|KGN57998.1\|	1.7e-50	99.01	hypothetical protein Csa_3G426350 [Cucumis sativus]	[more]
gi\|567910725\|ref\|XP_006447676.1\|	1.7e-08	46.67	hypothetical protein CICLE_v10018043mg [Citrus clementina]	[more]
gi\|18407238\|ref\|NP_564779.1\|	1.5e-06	42.11	protein RALF-like 9 [Arabidopsis thaliana]	[more]
gi\|565488585\|ref\|XP_006301932.1\|	2.0e-06	41.10	hypothetical protein CARUB_v10022412mg [Capsella rubella]	[more]
gi\|21592626\|gb\|AAM64575.1\|	3.4e-06	42.11	unknown [Arabidopsis thaliana]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR008801	RALF

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsa.017030	Cucsa.017030	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsa.017030.1	Cucsa.017030.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.017030.1.CDS.1	Cucsa.017030.1.CDS.1	CDS

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 26..72 score: 2.
None	No IPR available	PANTHER	PTHR34270	FAMILY NOT NAMED	coord: 1..84 score: 2.8
None	No IPR available	PANTHER	PTHR34270:SF1	PROTEIN RALF-LIKE 10-RELATED	coord: 1..84 score: 2.8