Cucsa.012570.2 (mRNA) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.012570.2
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Major latex-like protein
Location	scaffold00164 : 158890 .. 160252 (-)
Sequence length	840

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTATCACATCAAATAAAAATGAGCCGAAGTGATAGCATTGTTGCAAAGGTTGAGCTAAAATCTCATATTGAGAAGTTCTATGGCTTCTTCAGAAACCATGTGGAAGATTTGATGAATCTATTTCCTGATTTGTACCAAGGCATTGATCTTGTAGAAGGGCAATATTTATCTGCTGGATCTGTTATACTATTTAAATACCATCTTGGTGAGAATACTACTTTTTAATTTTCTCTTAATATATATATATATATATTCTTCTCTCCTAGTTAATACATTTATATTCCATCCTATTCTCCATTTCATTTAGGTTTCCTTGGGGACAAAATTCTTAAAATATAACTAATTTTGGCTACGTAATTTATTGACATGAGTTTGCTCAAGGGTTAAGAAGGAATGAGATATCAATCTATATAACTAACTACCTAAGACTTAATATCTCAAGATTAAGGGGGGAAATTTTGAAAAGGGTGCCAAAAACAGACTCCAAATTGTTGTGGTCAAAATATATTGACATATCTATTATTTTACTACCTCCTTCCACTCTTTAAAAAAAGAAAGATAGGGTTACTGTAAAAAAAATCTCATTCTTAAATAGTCGAATTTTACATCTAATATTGAACTTAAAGACATTCAATTTTAAGCCTATCAGATTCTTTTATATATATCCGTGTGTGAGTTTTTTAATTTTTAATTTAACGATCAAACAGGAGCTGATCAAGTTGTTTCAGAAAAATGGTTAATTAGAGCTGTGGATGATGCAAAAAAATGCATAATCTACGAAGCTATTGAAGGAGATTTGCAAAAGTATTACAAAGTTCTTAGAGCAAAGCTGGAAGTTGTTCATGGAAGATCAAGCAAAATAGGAAGAGGAAGCTTCGTAAAATGGACGATTGAGTTTGAAAAGGCTAATGAGAATGTGCCTTCACCTGACAGCCACATGGAAATTTTTGTCAAAATTTCCAAAGGAGTTGATGCTTATTGTCTTTCAAAACAAGGTAACTAAAACCCTAAAATCCCAACTAATTAAGTTCGTAAGTGTGTGTGAAACCTAAACTATGCCTCTTGTTTTAATTTATGTACAAGGTTTGTTCCCAAATAAGAAATTGTGTGTAATGTGATATATGTACACTAAGAATAATTATGCAAGTGGGATTTAGGGAAATGTGTGTATCTGATGTAATCTGTAATATTTTGAAGATGAAAAATCTACCTGTATGTGTTTTTTTCTTTTTTTTTTTTGTATTTCTGTGAAGGTATTGAATTGTTGAAGTTTAACCTTACAGAACAAAAGTATTTCCGATTAAACACGTGGTGGTTCAATGCATATAAGTTTAAAAGTCGTATCAATAAAATTGTGAGATGT

mRNA sequence

TTATCACATCAAATAAAAATGAGCCGAAGTGATAGCATTGTTGCAAAGGTTGAGCTAAAATCTCATATTGAGAAGTTCTATGGCTTCTTCAGAAACCATGTGGAAGATTTGATGAATCTATTTCCTGATTTGTACCAAGGCATTGATCTTGTAGAAGGGCAATATTTATCTGCTGGATCTGTTATACTATTTAAATACCATCTTGAAAAATGGTTAATTAGAGCTGTGGATGATGCAAAAAAATGCATAATCTACGAAGCTATTGAAGGAGATTTGCAAAAGTATTACAAAGTTCTTAGAGCAAAGCTGGAAGTTGTTCATGGAAGATCAAGCAAAATAGGAAGAGGAAGCTTCGTAAAATGGACGATTGAGTTTGAAAAGGCTAATGAGAATGTGCCTTCACCTGACAGCCACATGGAAATTTTTGTCAAAATTTCCAAAGGAGTTGATGCTTATTGTCTTTCAAAACAAGGTAACTAAAACCCTAAAATCCCAACTAATTAAGTTCGTAAGTGTGTGTGAAACCTAAACTATGCCTCTTGTTTTAATTTATGTACAAGGTTTGTTCCCAAATAAGAAATTGTGTGTAATGTGATATATGTACACTAAGAATAATTATGCAAGTGGGATTTAGGGAAATGTGTGTATCTGATGTAATCTGTAATATTTTGAAGATGAAAAATCTACCTGTATGTGTTTTTTTCTTTTTTTTTTTTGTATTTCTGTGAAGGTATTGAATTGTTGAAGTTTAACCTTACAGAACAAAAGTATTTCCGATTAAACACGTGGTGGTTCAATGCATATAAGTTTAAAAGTCGTATCAATAAAATTGTGAGATGT

Coding sequence (CDS)

ATGAGCCGAAGTGATAGCATTGTTGCAAAGGTTGAGCTAAAATCTCATATTGAGAAGTTCTATGGCTTCTTCAGAAACCATGTGGAAGATTTGATGAATCTATTTCCTGATTTGTACCAAGGCATTGATCTTGTAGAAGGGCAATATTTATCTGCTGGATCTGTTATACTATTTAAATACCATCTTGAAAAATGGTTAATTAGAGCTGTGGATGATGCAAAAAAATGCATAATCTACGAAGCTATTGAAGGAGATTTGCAAAAGTATTACAAAGTTCTTAGAGCAAAGCTGGAAGTTGTTCATGGAAGATCAAGCAAAATAGGAAGAGGAAGCTTCGTAAAATGGACGATTGAGTTTGAAAAGGCTAATGAGAATGTGCCTTCACCTGACAGCCACATGGAAATTTTTGTCAAAATTTCCAAAGGAGTTGATGCTTATTGTCTTTCAAAACAAGGTAACTAA

Protein sequence

MSRSDSIVAKVELKSHIEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVILFKYHLEKWLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVVHGRSSKIGRGSFVKWTIEFEKANENVPSPDSHMEIFVKISKGVDAYCLSKQGN*

Homology

[Close]

BLAST of Cucsa.012570.2 vs. Swiss-Prot
Match: MLP34_ARATH (MLP-like protein 34 OS=Arabidopsis thaliana GN=MLP34 PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 3.3e-16
Identity = 51/156 (32.69%), Postives = 82/156 (52.56%), Query Frame = 1


Query: 4   SDSIVAKVELKSHIEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVILFKY--- 63
           ++++  +VE+K+  EKF+  F      +    P   Q  DL EG + + GS++ + Y   
Sbjct: 166 TETLETEVEIKASAEKFHHMFAGKPHHVSKATPGNIQSCDLHEGDWGTVGSIVFWNYVHD 225

Query: 64  ---HLEKWLIRAVDDAKKCIIYEAIEGDLQKYYK--VLRAKLEVVHGRSSKIGRGSFVKW 123
               + K  I AVD  K  I +  IEGDL K YK  V+  ++   HG     G GS V W
Sbjct: 226 GEAKVAKERIEAVDPEKNLITFRVIEGDLMKEYKSFVITIQVTPKHG-----GSGSVVHW 285

Query: 124 TIEFEKANENVPSPDSHMEIFVKISKGVDAYCLSKQ 152
             E+EK NE V  P++ ++  V++SK +D + L+++
Sbjct: 286 HFEYEKINEEVAHPETLLQFAVEVSKEIDEHLLAEE 316

HSP 2 Score: 76.6 bits (187), Expect = 2.6e-13
Identity = 46/150 (30.67%), Postives = 77/150 (51.33%), Query Frame = 1


Query: 10  KVELKSHIEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVILFKY------HLE 69
           +VE+K+   +F+  F      +    P   Q  DL EG + + GS++ + Y       + 
Sbjct: 16  EVEIKASAGQFHHMFAGKPHHVSKASPGNIQSCDLHEGDWGTVGSIVFWNYVHDGEAKVA 75

Query: 70  KWLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVV--HGRSSKIGRGSFVKWTIEFEK 129
           K  I AV+  K  I +  IEGDL K YK     ++V   HG     G GS V W +E+EK
Sbjct: 76  KERIEAVEPEKNLITFRVIEGDLMKEYKSFLITIQVTPKHG-----GPGSIVHWHLEYEK 135

Query: 130 ANENVPSPDSHMEIFVKISKGVDAYCLSKQ 152
            ++ V  P++ ++  V++S+ +D + LS++
Sbjct: 136 ISDEVAHPETLLQFCVEVSQEIDEHLLSEE 160

[Close]

BLAST of Cucsa.012570.2 vs. Swiss-Prot
Match: KIRO_ACTDE (Kirola OS=Actinidia deliciosa PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 9.5e-16
Identity = 49/154 (31.82%), Postives = 82/154 (53.25%), Query Frame = 1


Query: 1   MSRSDSIVAKVELKSHIEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVILFKY 60
           M  S  +V +VE+ S    FY  FR  +  +  + P   QG+DL+EG + + GSVI FKY
Sbjct: 1   MDLSGKMVKQVEILSDGIVFYEIFRYRLYLISEMSPVNIQGVDLLEGNWGTVGSVIFFKY 60

Query: 61  HLE------KWLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVVHGRSSKIGRGSFVK 120
            ++      K ++ A+D+  K + ++ +EGDL + YK     ++V        G  + V 
Sbjct: 61  TIDGKEKTAKDIVEAIDEETKSVTFKIVEGDLMELYKTFIIIVQV-----DTKGEHNSVT 120

Query: 121 WTIEFEKANENVPSPDSHMEIFVKISKGVDAYCL 149
           WT  +EK  E+V  P++ M   ++I+K ++ Y L
Sbjct: 121 WTFHYEKLKEDVEEPNTLMNFCIEITKDIETYHL 149

[Close]

BLAST of Cucsa.012570.2 vs. Swiss-Prot
Match: ML165_ARATH (MLP-like protein 165 OS=Arabidopsis thaliana GN=MLP165 PE=2 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 2.8e-15
Identity = 48/154 (31.17%), Postives = 85/154 (55.19%), Query Frame = 1


Query: 5   DSIVAKVELKSHIEKFYGFFRN--HVEDLMNLFPDLYQGIDLVEGQYLSAGSVILFKY-- 64
           + I   V++K+  +KF+ F R   HV    +      +G DL+EG++   GS++L+K   
Sbjct: 4   EEIEVDVDIKTRADKFHKFIRRSQHVPKATHYI----KGCDLLEGEWGKVGSILLWKLVF 63

Query: 65  ----HLEKWLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVVHGRSSKIGRGSFVKWT 124
                + K +I  +D+ K  I    +EG L+K YK     ++V+  +    G GS VKW 
Sbjct: 64  DGEPRVSKDMIEVIDEEKNVIQLRVLEGPLKKEYKSFLKTMKVMSPKHG--GPGSVVKWN 123

Query: 125 IEFEKANENVPSPDSHMEIFVKISKGVDAYCLSK 151
           +++E+ ++NV  P+  ++ FV+++K +D Y LSK
Sbjct: 124 MKYERIDQNVDHPNRLLQFFVEVTKEIDQYLLSK 151

[Close]

BLAST of Cucsa.012570.2 vs. Swiss-Prot
Match: MLP43_ARATH (MLP-like protein 43 OS=Arabidopsis thaliana GN=MLP43 PE=1 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 2.8e-15
Identity = 50/161 (31.06%), Postives = 83/161 (51.55%), Query Frame = 1


Query: 1   MSRSDSIVAK----VELKSHIEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVI 60
           M+ + S+V K    VE+K+  +KF+  F      +    PD   G +L EG +   GS++
Sbjct: 1   MAEASSLVGKLETEVEIKASAKKFHHMFTERPHHVSKATPDKIHGCELHEGDWGKVGSIV 60

Query: 61  LFKY------HLEKWLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVVHGRSSKIGRG 120
           ++KY       + K  I AVD  K  I ++ +EGDL   YK     L+V   +      G
Sbjct: 61  IWKYVHDGKLTVGKNKIEAVDPEKNLITFKVLEGDLMNEYKSFAFTLQVTPKQGES---G 120

Query: 121 SFVKWTIEFEKANENVPSPDSHMEIFVKISKGVDAYCLSKQ 152
           S   W +E+EK +E V  P++ ++  V+ISK +D + L+++
Sbjct: 121 SIAHWHLEYEKISEEVAHPETLLQFCVEISKEIDEHLLAEE 158

[Close]

BLAST of Cucsa.012570.2 vs. Swiss-Prot
Match: MLP28_ARATH (MLP-like protein 28 OS=Arabidopsis thaliana GN=MLP28 PE=1 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 8.0e-15
Identity = 50/160 (31.25%), Postives = 82/160 (51.25%), Query Frame = 1


Query: 2   SRSDSIVAK----VELKSHIEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVIL 61
           + + S+V K    VE+K+  +KF+  F      +    P   QG DL EG + + GS++ 
Sbjct: 17  TEASSLVGKLETDVEIKASADKFHHMFAGKPHHVSKASPGNIQGCDLHEGDWGTVGSIVF 76

Query: 62  FKY------HLEKWLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVVHGRSSKIGRGS 121
           + Y       + K  I AV+  K  I +  IEGDL K YK     ++V        G GS
Sbjct: 77  WNYVHDGEAKVAKERIEAVEPDKNLITFRVIEGDLMKEYKSFLLTIQVT---PKPGGPGS 136

Query: 122 FVKWTIEFEKANENVPSPDSHMEIFVKISKGVDAYCLSKQ 152
            V W +E+EK +E V  P++ ++  V++SK +D + L+++
Sbjct: 137 IVHWHLEYEKISEEVAHPETLLQFCVEVSKEIDEHLLAEE 173

HSP 2 Score: 80.5 bits (197), Expect = 1.8e-14
Identity = 48/148 (32.43%), Postives = 78/148 (52.70%), Query Frame = 1


Query: 11  VELKSHIEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVILFKY------HLEK 70
           VE+K+  EKF+  F      +    P   QG DL EG +   GS++ + Y       + K
Sbjct: 192 VEIKASAEKFHHMFAGKPHHVSKASPGNIQGCDLHEGDWGQVGSIVFWNYVHDREAKVAK 251

Query: 71  WLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVVHGRSSKI-GRGSFVKWTIEFEKAN 130
             I AV+  K  I +  I+GDL K YK     ++V    + K+ G GS V W +E+EK +
Sbjct: 252 ERIEAVEPNKNLITFRVIDGDLMKEYKSFLLTIQV----TPKLGGPGSIVHWHLEYEKIS 311

Query: 131 ENVPSPDSHMEIFVKISKGVDAYCLSKQ 152
           E V  P++ ++  V++SK +D + L+++
Sbjct: 312 EEVAHPETLLQFCVEVSKEIDEHLLAEE 335

[Close]

BLAST of Cucsa.012570.2 vs. TrEMBL
Match: A0A0A0LAY7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G342350 PE=4 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 2.1e-78
Identity = 151/160 (94.38%), Postives = 152/160 (95.00%), Query Frame = 1


Query: 1   MSRSDSIVAKVELKSHIEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVILFKY 60
           MSRSDSIVAKVELKS+IEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVILFKY
Sbjct: 1   MSRSDSIVAKVELKSNIEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVILFKY 60

Query: 61  HL-------EKWLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVVHGRSSKIGRGSFV 120
           HL       EKWLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVVHGRSSKIGRGSF 
Sbjct: 61  HLGADQVVSEKWLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVVHGRSSKIGRGSFA 120

Query: 121 KWTIEFEKANENVPSPDSHMEIFVKISKGVDAYCLSKQGN 154
           KWTIEFEKANENVPSPDSHMEIFVKISKGVDAYCLSKQGN
Sbjct: 121 KWTIEFEKANENVPSPDSHMEIFVKISKGVDAYCLSKQGN 160

[Close]

BLAST of Cucsa.012570.2 vs. TrEMBL
Match: N0DK19_CUCPE (Major latex-like protein OS=Cucurbita pepo subsp. pepo GN=MLP-GR1 PE=2 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 2.1e-46
Identity = 96/152 (63.16%), Postives = 118/152 (77.63%), Query Frame = 1


Query: 1   MSRSDSIVAKVELKSHIEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVILFKY 60
           M ++DSI  KV+LKS  EK YGFFRNH+ DL++LFP+ YQ I LVEGQ+ S+GSV+ FK+
Sbjct: 1   MVQTDSIWVKVDLKSSPEKVYGFFRNHLGDLVDLFPETYQSIQLVEGQHFSSGSVVQFKF 60

Query: 61  HL------EKWLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVVHGRSSKIGRGSFVK 120
                   EKW IR VDD KK IIYEA+EGD  K +KVLRAK EVV+G  SK+ RG+F K
Sbjct: 61  QFGDELRAEKWAIRVVDDVKKYIIYEAVEGDPLKEFKVLRAKFEVVNGGLSKVRRGNFTK 120

Query: 121 WTIEFEKANENVPSPDSHMEIFVKISKGVDAY 147
           WT+EFEKAN+NV SP +++E+FVKISKGVDAY
Sbjct: 121 WTVEFEKANQNVASPQNYLELFVKISKGVDAY 152

[Close]

BLAST of Cucsa.012570.2 vs. TrEMBL
Match: N0DKL1_CUCPE (Major latex-like protein OS=Cucurbita pepo subsp. ovifera GN=MLP-PG1 PE=2 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 2.1e-46
Identity = 96/152 (63.16%), Postives = 118/152 (77.63%), Query Frame = 1


Query: 1   MSRSDSIVAKVELKSHIEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVILFKY 60
           M ++DSI  KV+LKS  EK YGFFRNH+ DL++LFP+ YQ I LVEGQ+ S+GSV+ FK+
Sbjct: 1   MVQTDSIWVKVDLKSSPEKVYGFFRNHLGDLVDLFPETYQSIQLVEGQHFSSGSVVQFKF 60

Query: 61  HL------EKWLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVVHGRSSKIGRGSFVK 120
                   EKW IR VDD KK IIYEA+EGD  K +KVLRAK EVV+G  SK+ RG+F K
Sbjct: 61  QFGDELRAEKWAIRVVDDVKKYIIYEAVEGDPLKEFKVLRAKFEVVNGGLSKVRRGNFTK 120

Query: 121 WTIEFEKANENVPSPDSHMEIFVKISKGVDAY 147
           WT+EFEKAN+NV SP +++E+FVKISKGVDAY
Sbjct: 121 WTVEFEKANQNVASPQNYLELFVKISKGVDAY 152

[Close]

BLAST of Cucsa.012570.2 vs. TrEMBL
Match: A0A0A0M0E1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G680110 PE=4 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 5.2e-45
Identity = 91/156 (58.33%), Postives = 122/156 (78.21%), Query Frame = 1


Query: 1   MSRSDSIVAKVELK-SHIEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVILFK 60
           M  SDSI  K +LK S  EKFYGF RN + DL+ +FPD+Y+ I++VEG+  S+GSV+ F+
Sbjct: 1   MMESDSIEKKFKLKHSSAEKFYGFLRNDLGDLVKMFPDVYKTIEVVEGRTFSSGSVVHFQ 60

Query: 61  YHL------EKWLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVVHGRSSKIGRGSFV 120
           YHL      EKWLI+  DDAKKCI+YEA+EGD+  YYK+LRA++E V+GRS++IG  SF 
Sbjct: 61  YHLGDQVVSEKWLIKVADDAKKCIVYEAVEGDMLNYYKMLRARVEAVNGRSNEIGE-SFA 120

Query: 121 KWTIEFEKANENVPSPDSHMEIFVKISKGVDAYCLS 150
           +WT+EFEKA+ENVP P +H+++FV++SK VDAYC S
Sbjct: 121 EWTVEFEKADENVPLPQTHLDLFVEMSKAVDAYCFS 155

[Close]

BLAST of Cucsa.012570.2 vs. TrEMBL
Match: N0DK07_CUCPE (Major latex-like protein OS=Cucurbita pepo subsp. pepo GN=MLP-GR3 PE=2 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 2.6e-44
Identity = 92/152 (60.53%), Postives = 118/152 (77.63%), Query Frame = 1


Query: 1   MSRSDSIVAKVELKSHIEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVILFKY 60
           M ++DSI  KV+LKS  EK YGFFRNH+ DL++LFP+ +  I LVEGQ+   GS++L KY
Sbjct: 1   MVQTDSIWVKVDLKSSPEKVYGFFRNHLGDLVDLFPENFNSIQLVEGQHFDRGSLVLSKY 60

Query: 61  H------LEKWLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVVHGRSSKIGRGSFVK 120
                  +EKW+IRAVDD KK I+YEA+EG+  K +KVLRAK+E VHG S+K+G G+F K
Sbjct: 61  EFGHEHRVEKWVIRAVDDVKKYIVYEAVEGEALKQFKVLRAKVEAVHGGSTKVGGGNFTK 120

Query: 121 WTIEFEKANENVPSPDSHMEIFVKISKGVDAY 147
            TIEFEKANENV SP+ ++E+FVKI+KGVDAY
Sbjct: 121 LTIEFEKANENVASPEIYLELFVKIAKGVDAY 152

[Close]

BLAST of Cucsa.012570.2 vs. TAIR10
Match: AT1G70850.1 (AT1G70850.1 MLP-like protein 34)

HSP 1 Score: 86.3 bits (212), Expect = 1.8e-17
Identity = 51/156 (32.69%), Postives = 82/156 (52.56%), Query Frame = 1


Query: 4   SDSIVAKVELKSHIEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVILFKY--- 63
           ++++  +VE+K+  EKF+  F      +    P   Q  DL EG + + GS++ + Y   
Sbjct: 166 TETLETEVEIKASAEKFHHMFAGKPHHVSKATPGNIQSCDLHEGDWGTVGSIVFWNYVHD 225

Query: 64  ---HLEKWLIRAVDDAKKCIIYEAIEGDLQKYYK--VLRAKLEVVHGRSSKIGRGSFVKW 123
               + K  I AVD  K  I +  IEGDL K YK  V+  ++   HG     G GS V W
Sbjct: 226 GEAKVAKERIEAVDPEKNLITFRVIEGDLMKEYKSFVITIQVTPKHG-----GSGSVVHW 285

Query: 124 TIEFEKANENVPSPDSHMEIFVKISKGVDAYCLSKQ 152
             E+EK NE V  P++ ++  V++SK +D + L+++
Sbjct: 286 HFEYEKINEEVAHPETLLQFAVEVSKEIDEHLLAEE 316

HSP 2 Score: 76.6 bits (187), Expect = 1.5e-14
Identity = 46/150 (30.67%), Postives = 77/150 (51.33%), Query Frame = 1


Query: 10  KVELKSHIEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVILFKY------HLE 69
           +VE+K+   +F+  F      +    P   Q  DL EG + + GS++ + Y       + 
Sbjct: 16  EVEIKASAGQFHHMFAGKPHHVSKASPGNIQSCDLHEGDWGTVGSIVFWNYVHDGEAKVA 75

Query: 70  KWLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVV--HGRSSKIGRGSFVKWTIEFEK 129
           K  I AV+  K  I +  IEGDL K YK     ++V   HG     G GS V W +E+EK
Sbjct: 76  KERIEAVEPEKNLITFRVIEGDLMKEYKSFLITIQVTPKHG-----GPGSIVHWHLEYEK 135

Query: 130 ANENVPSPDSHMEIFVKISKGVDAYCLSKQ 152
            ++ V  P++ ++  V++S+ +D + LS++
Sbjct: 136 ISDEVAHPETLLQFCVEVSQEIDEHLLSEE 160

[Close]

BLAST of Cucsa.012570.2 vs. TAIR10
Match: AT1G35260.1 (AT1G35260.1 MLP-like protein 165)

HSP 1 Score: 83.2 bits (204), Expect = 1.6e-16
Identity = 48/154 (31.17%), Postives = 85/154 (55.19%), Query Frame = 1


Query: 5   DSIVAKVELKSHIEKFYGFFRN--HVEDLMNLFPDLYQGIDLVEGQYLSAGSVILFKY-- 64
           + I   V++K+  +KF+ F R   HV    +      +G DL+EG++   GS++L+K   
Sbjct: 4   EEIEVDVDIKTRADKFHKFIRRSQHVPKATHYI----KGCDLLEGEWGKVGSILLWKLVF 63

Query: 65  ----HLEKWLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVVHGRSSKIGRGSFVKWT 124
                + K +I  +D+ K  I    +EG L+K YK     ++V+  +    G GS VKW 
Sbjct: 64  DGEPRVSKDMIEVIDEEKNVIQLRVLEGPLKKEYKSFLKTMKVMSPKHG--GPGSVVKWN 123

Query: 125 IEFEKANENVPSPDSHMEIFVKISKGVDAYCLSK 151
           +++E+ ++NV  P+  ++ FV+++K +D Y LSK
Sbjct: 124 MKYERIDQNVDHPNRLLQFFVEVTKEIDQYLLSK 151

[Close]

BLAST of Cucsa.012570.2 vs. TAIR10
Match: AT1G70890.1 (AT1G70890.1 MLP-like protein 43)

HSP 1 Score: 83.2 bits (204), Expect = 1.6e-16
Identity = 50/161 (31.06%), Postives = 83/161 (51.55%), Query Frame = 1


Query: 1   MSRSDSIVAK----VELKSHIEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVI 60
           M+ + S+V K    VE+K+  +KF+  F      +    PD   G +L EG +   GS++
Sbjct: 1   MAEASSLVGKLETEVEIKASAKKFHHMFTERPHHVSKATPDKIHGCELHEGDWGKVGSIV 60

Query: 61  LFKY------HLEKWLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVVHGRSSKIGRG 120
           ++KY       + K  I AVD  K  I ++ +EGDL   YK     L+V   +      G
Sbjct: 61  IWKYVHDGKLTVGKNKIEAVDPEKNLITFKVLEGDLMNEYKSFAFTLQVTPKQGES---G 120

Query: 121 SFVKWTIEFEKANENVPSPDSHMEIFVKISKGVDAYCLSKQ 152
           S   W +E+EK +E V  P++ ++  V+ISK +D + L+++
Sbjct: 121 SIAHWHLEYEKISEEVAHPETLLQFCVEISKEIDEHLLAEE 158

[Close]

BLAST of Cucsa.012570.2 vs. TAIR10
Match: AT1G70830.1 (AT1G70830.1 MLP-like protein 28)

HSP 1 Score: 81.6 bits (200), Expect = 4.5e-16
Identity = 50/160 (31.25%), Postives = 82/160 (51.25%), Query Frame = 1


Query: 2   SRSDSIVAK----VELKSHIEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVIL 61
           + + S+V K    VE+K+  +KF+  F      +    P   QG DL EG + + GS++ 
Sbjct: 17  TEASSLVGKLETDVEIKASADKFHHMFAGKPHHVSKASPGNIQGCDLHEGDWGTVGSIVF 76

Query: 62  FKY------HLEKWLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVVHGRSSKIGRGS 121
           + Y       + K  I AV+  K  I +  IEGDL K YK     ++V        G GS
Sbjct: 77  WNYVHDGEAKVAKERIEAVEPDKNLITFRVIEGDLMKEYKSFLLTIQVT---PKPGGPGS 136

Query: 122 FVKWTIEFEKANENVPSPDSHMEIFVKISKGVDAYCLSKQ 152
            V W +E+EK +E V  P++ ++  V++SK +D + L+++
Sbjct: 137 IVHWHLEYEKISEEVAHPETLLQFCVEVSKEIDEHLLAEE 173

HSP 2 Score: 80.5 bits (197), Expect = 1.0e-15
Identity = 48/148 (32.43%), Postives = 78/148 (52.70%), Query Frame = 1


Query: 11  VELKSHIEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVILFKY------HLEK 70
           VE+K+  EKF+  F      +    P   QG DL EG +   GS++ + Y       + K
Sbjct: 192 VEIKASAEKFHHMFAGKPHHVSKASPGNIQGCDLHEGDWGQVGSIVFWNYVHDREAKVAK 251

Query: 71  WLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVVHGRSSKI-GRGSFVKWTIEFEKAN 130
             I AV+  K  I +  I+GDL K YK     ++V    + K+ G GS V W +E+EK +
Sbjct: 252 ERIEAVEPNKNLITFRVIDGDLMKEYKSFLLTIQV----TPKLGGPGSIVHWHLEYEKIS 311

Query: 131 ENVPSPDSHMEIFVKISKGVDAYCLSKQ 152
           E V  P++ ++  V++SK +D + L+++
Sbjct: 312 EEVAHPETLLQFCVEVSKEIDEHLLAEE 335

[Close]

BLAST of Cucsa.012570.2 vs. TAIR10
Match: AT1G70840.1 (AT1G70840.1 MLP-like protein 31)

HSP 1 Score: 79.3 bits (194), Expect = 2.2e-15
Identity = 45/147 (30.61%), Postives = 75/147 (51.02%), Query Frame = 1


Query: 11  VELKSHIEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVILFKY------HLEK 70
           +E+K+   KF+  F      +    P   QG +L EG +   GS++ + Y       + K
Sbjct: 28  IEIKASAGKFHHMFAGRPHHVSKATPGKIQGCELHEGDWGKVGSIVFWNYVHDGEAKVAK 87

Query: 71  WLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVVHGRSSKIGRGSFVKWTIEFEKANE 130
             I AV+  K  I +  IEGDL K YK     ++V   R    G GS V W +E+EK ++
Sbjct: 88  ERIEAVEPEKNLITFRVIEGDLLKEYKSFVITIQVTPKRG---GPGSVVHWHVEYEKIDD 147

Query: 131 NVPSPDSHMEIFVKISKGVDAYCLSKQ 152
            V  P++ ++  V++SK +D + L+++
Sbjct: 148 KVAHPETFLDFCVEVSKEIDEHLLNEE 171

[Close]

BLAST of Cucsa.012570.2 vs. NCBI nr
Match: gi|700202699|gb|KGN57832.1| (hypothetical protein Csa_3G342350 [Cucumis sativus])

HSP 1 Score: 299.7 bits (766), Expect = 3.0e-78
Identity = 151/160 (94.38%), Postives = 152/160 (95.00%), Query Frame = 1


Query: 1   MSRSDSIVAKVELKSHIEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVILFKY 60
           MSRSDSIVAKVELKS+IEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVILFKY
Sbjct: 1   MSRSDSIVAKVELKSNIEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVILFKY 60

Query: 61  HL-------EKWLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVVHGRSSKIGRGSFV 120
           HL       EKWLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVVHGRSSKIGRGSF 
Sbjct: 61  HLGADQVVSEKWLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVVHGRSSKIGRGSFA 120

Query: 121 KWTIEFEKANENVPSPDSHMEIFVKISKGVDAYCLSKQGN 154
           KWTIEFEKANENVPSPDSHMEIFVKISKGVDAYCLSKQGN
Sbjct: 121 KWTIEFEKANENVPSPDSHMEIFVKISKGVDAYCLSKQGN 160

[Close]

BLAST of Cucsa.012570.2 vs. NCBI nr
Match: gi|778688605|ref|XP_004149788.2| (PREDICTED: kirola-like [Cucumis sativus])

HSP 1 Score: 295.0 bits (754), Expect = 7.4e-77
Identity = 149/158 (94.30%), Postives = 150/158 (94.94%), Query Frame = 1


Query: 1   MSRSDSIVAKVELKSHIEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVILFKY 60
           MSRSDSIVAKVELKS+IEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVILFKY
Sbjct: 1   MSRSDSIVAKVELKSNIEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVILFKY 60

Query: 61  HL-------EKWLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVVHGRSSKIGRGSFV 120
           HL       EKWLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVVHGRSSKIGRGSF 
Sbjct: 61  HLGADQVVSEKWLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVVHGRSSKIGRGSFA 120

Query: 121 KWTIEFEKANENVPSPDSHMEIFVKISKGVDAYCLSKQ 152
           KWTIEFEKANENVPSPDSHMEIFVKISKGVDAYCLSKQ
Sbjct: 121 KWTIEFEKANENVPSPDSHMEIFVKISKGVDAYCLSKQ 158

[Close]

BLAST of Cucsa.012570.2 vs. NCBI nr
Match: gi|477504344|dbj|BAN14689.1| (major latex-like protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 193.4 bits (490), Expect = 3.0e-46
Identity = 96/152 (63.16%), Postives = 118/152 (77.63%), Query Frame = 1


Query: 1   MSRSDSIVAKVELKSHIEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVILFKY 60
           M ++DSI  KV+LKS  EK YGFFRNH+ DL++LFP+ YQ I LVEGQ+ S+GSV+ FK+
Sbjct: 1   MVQTDSIWVKVDLKSSPEKVYGFFRNHLGDLVDLFPETYQSIQLVEGQHFSSGSVVQFKF 60

Query: 61  HL------EKWLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVVHGRSSKIGRGSFVK 120
                   EKW IR VDD KK IIYEA+EGD  K +KVLRAK EVV+G  SK+ RG+F K
Sbjct: 61  QFGDELRAEKWAIRVVDDVKKYIIYEAVEGDPLKEFKVLRAKFEVVNGGLSKVRRGNFTK 120

Query: 121 WTIEFEKANENVPSPDSHMEIFVKISKGVDAY 147
           WT+EFEKAN+NV SP +++E+FVKISKGVDAY
Sbjct: 121 WTVEFEKANQNVASPQNYLELFVKISKGVDAY 152

[Close]

BLAST of Cucsa.012570.2 vs. NCBI nr
Match: gi|477504342|dbj|BAN14688.1| (major latex-like protein [Cucurbita pepo subsp. ovifera])

HSP 1 Score: 193.4 bits (490), Expect = 3.0e-46
Identity = 96/152 (63.16%), Postives = 118/152 (77.63%), Query Frame = 1


Query: 1   MSRSDSIVAKVELKSHIEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVILFKY 60
           M ++DSI  KV+LKS  EK YGFFRNH+ DL++LFP+ YQ I LVEGQ+ S+GSV+ FK+
Sbjct: 1   MVQTDSIWVKVDLKSSPEKVYGFFRNHLGDLVDLFPETYQSIQLVEGQHFSSGSVVQFKF 60

Query: 61  HL------EKWLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVVHGRSSKIGRGSFVK 120
                   EKW IR VDD KK IIYEA+EGD  K +KVLRAK EVV+G  SK+ RG+F K
Sbjct: 61  QFGDELRAEKWAIRVVDDVKKYIIYEAVEGDPLKEFKVLRAKFEVVNGGLSKVRRGNFTK 120

Query: 121 WTIEFEKANENVPSPDSHMEIFVKISKGVDAY 147
           WT+EFEKAN+NV SP +++E+FVKISKGVDAY
Sbjct: 121 WTVEFEKANQNVASPQNYLELFVKISKGVDAY 152

[Close]

BLAST of Cucsa.012570.2 vs. NCBI nr
Match: gi|449449571|ref|XP_004142538.1| (PREDICTED: kirola-like [Cucumis sativus])

HSP 1 Score: 188.7 bits (478), Expect = 7.4e-45
Identity = 91/156 (58.33%), Postives = 122/156 (78.21%), Query Frame = 1


Query: 1   MSRSDSIVAKVELK-SHIEKFYGFFRNHVEDLMNLFPDLYQGIDLVEGQYLSAGSVILFK 60
           M  SDSI  K +LK S  EKFYGF RN + DL+ +FPD+Y+ I++VEG+  S+GSV+ F+
Sbjct: 1   MMESDSIEKKFKLKHSSAEKFYGFLRNDLGDLVKMFPDVYKTIEVVEGRTFSSGSVVHFQ 60

Query: 61  YHL------EKWLIRAVDDAKKCIIYEAIEGDLQKYYKVLRAKLEVVHGRSSKIGRGSFV 120
           YHL      EKWLI+  DDAKKCI+YEA+EGD+  YYK+LRA++E V+GRS++IG  SF 
Sbjct: 61  YHLGDQVVSEKWLIKVADDAKKCIVYEAVEGDMLNYYKMLRARVEAVNGRSNEIGE-SFA 120

Query: 121 KWTIEFEKANENVPSPDSHMEIFVKISKGVDAYCLS 150
           +WT+EFEKA+ENVP P +H+++FV++SK VDAYC S
Sbjct: 121 EWTVEFEKADENVPLPQTHLDLFVEMSKAVDAYCFS 155

The following BLAST results are available for this feature:

BLAST of Cucsa.012570.2 vs. Swiss-Prot


Match Name	E-value	Identity	Description
MLP34_ARATH	3.3e-16	32.69	MLP-like protein 34 OS=Arabidopsis thaliana GN=MLP34 PE=2 SV=1	[more]
KIRO_ACTDE	9.5e-16	31.82	Kirola OS=Actinidia deliciosa PE=1 SV=1	[more]
ML165_ARATH	2.8e-15	31.17	MLP-like protein 165 OS=Arabidopsis thaliana GN=MLP165 PE=2 SV=1	[more]
MLP43_ARATH	2.8e-15	31.06	MLP-like protein 43 OS=Arabidopsis thaliana GN=MLP43 PE=1 SV=1	[more]
MLP28_ARATH	8.0e-15	31.25	MLP-like protein 28 OS=Arabidopsis thaliana GN=MLP28 PE=1 SV=1	[more]

BLAST of Cucsa.012570.2 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LAY7_CUCSA	2.1e-78	94.38	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G342350 PE=4 SV=1	[more]
N0DK19_CUCPE	2.1e-46	63.16	Major latex-like protein OS=Cucurbita pepo subsp. pepo GN=MLP-GR1 PE=2 SV=1	[more]
N0DKL1_CUCPE	2.1e-46	63.16	Major latex-like protein OS=Cucurbita pepo subsp. ovifera GN=MLP-PG1 PE=2 SV=1	[more]
A0A0A0M0E1_CUCSA	5.2e-45	58.33	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G680110 PE=4 SV=1	[more]
N0DK07_CUCPE	2.6e-44	60.53	Major latex-like protein OS=Cucurbita pepo subsp. pepo GN=MLP-GR3 PE=2 SV=1	[more]

BLAST of Cucsa.012570.2 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G70850.1	1.8e-17	32.69	MLP-like protein 34	[more]
AT1G35260.1	1.6e-16	31.17	MLP-like protein 165	[more]
AT1G70890.1	1.6e-16	31.06	MLP-like protein 43	[more]
AT1G70830.1	4.5e-16	31.25	MLP-like protein 28	[more]
AT1G70840.1	2.2e-15	30.61	MLP-like protein 31	[more]

BLAST of Cucsa.012570.2 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700202699\|gb\|KGN57832.1\|	3.0e-78	94.38	hypothetical protein Csa_3G342350 [Cucumis sativus]	[more]
gi\|778688605\|ref\|XP_004149788.2\|	7.4e-77	94.30	PREDICTED: kirola-like [Cucumis sativus]	[more]
gi\|477504344\|dbj\|BAN14689.1\|	3.0e-46	63.16	major latex-like protein [Cucurbita pepo subsp. pepo]	[more]
gi\|477504342\|dbj\|BAN14688.1\|	3.0e-46	63.16	major latex-like protein [Cucurbita pepo subsp. ovifera]	[more]
gi\|449449571\|ref\|XP_004142538.1\|	7.4e-45	58.33	PREDICTED: kirola-like [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR000916	Bet_v_I/MLP
IPR023393	START-like_dom_sf

Vocabulary: **Biological Process**
Term	Definition
GO:0006952	defense response
GO:0009607	response to biotic stimulus

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0009607	response to biotic stimulus
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsa.012570	Cucsa.012570	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsa.012570.2	Cucsa.012570.2-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.012570.2.CDS.2	Cucsa.012570.2.CDS.2	CDS
Cucsa.012570.2.CDS.1	Cucsa.012570.2.CDS.1	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.012570.2.three_prime_UTR.1	Cucsa.012570.2.three_prime_UTR.1	three_prime_UTR

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.012570.2.five_prime_UTR.1	Cucsa.012570.2.five_prime_UTR.1	five_prime_UTR

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000916	Bet v I/Major latex protein	PFAM	PF00407	Bet_v_1	coord: 4..149 score: 1.6
IPR000916	Bet v I/Major latex protein	SMART	SM01037	Bet_v_1_2	coord: 2..150 score: 6.2
IPR023393	START-like domain	GENE3D	G3DSA:3.30.530.20		coord: 3..150 score: 3.3
None	No IPR available	PANTHER	PTHR31907	FAMILY NOT NAMED	coord: 2..151 score: 2.2
None	No IPR available	unknown	SSF55961	Bet v1-like	coord: 63..148 score: 2.25E-23coord: 1..35 score: 2.25