Cucsa.007350.1 (mRNA) Cucumber (Gy14) v1

NameCucsa.007350.1
TypemRNA
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionKinase family protein
Locationscaffold00089 : 269340 .. 270835 (-)
Sequence length1230
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACTGGACCAGAGGCCCCCCCGTCGGCCGCGGCTCCTCCGCCACCGTCTATATGGCTACAATCAACTCATCCGGCCAACAATTCGCCGTCAAATCCACCGACTTTGCATCATCGGCTCTGTTGCAGAAGGAACACGCTCTTCTCTCCAATTTGAGCTCCCCATACCTAATTAAGTCCCTAGGTTTCGACGTTGAAACCCAACCCTTCAATCCCCCGGTGTTCAATCTCTTCATGGAGTATTTGCCGGAGGGGACTCTGTGGGACGCGATTCAGAGCAACGGCGGTCGAATTGAGGAATCGATGATCCGAGTGTTTTCGCGGCAGATTCTCAAAGGATTGGACTACCTTCATGCTAATGGATTGGCACATTGCGATATCAAGAGCCGGAATCTGTTACTGAGTGATGGGGATTTGAAAATTGCCGATCTAGGTTGTGCGAAATTTGTGGATGGAATTTCCGGTAATGGAGTTGGGGCTTTTTCTGGTTCGCCGGCGTTCATGGCGCCGGAAGTTGCAAGAGGTGAAGAACAGGGATTTCCGGCCGATGTTTGGGCTTTTGGATGTACAGTCATTGAAATGGCTACTGGGGATCATCCATGGCCGGAAATAGAGGATCCGGTTTCGGCGTTGTATCGGATTGGATTTTCCGGCGAGTTACCAGAGATTCCGCGGTGGTTGTCGGAAATTGCGAGGGATTTTGTCGCCAAGTGTTTGATAAAAGACTCGAAAGAAAGATGGAGTGTGAAACGGCTTCTCGAGCATCCGTTTCTTCAAGAACTTGATTCTGAATCGGAAATTGAAGGGTCGAGGATGAAAATGGTGAATTCTTCTTCTCCGAGCTGTGTTTTGGATCAGAGGTTTTGGGATTCAATGGAAGATTTGGAGACTCCATGGAAGGTGACTCAACAAGCTTCTTTGGTGGATTGTCCAGCTGAGAGGATTAGAAGGTTGGCTGATAATTTCTCTTGCGATTTGGAATTGGAATGGGATGATGGAGACTGGGTTTTGATCAGAAACAATGGGGTCGGCGGTGGAAGCCATGGAAATGGATCTGTGAGCATAGATTTTTGTGCTACAGTTGAAGAAGAAGATGAAAATTTGAACTCGGAAATTGAAGAAAATTCTGTATTGTTCTGTTTAGTTGATGATGATTTTAGTGTTGTGAACAGTAGGAAAGGAGGATTACAAATGGAATTAAAGAGTGATTTTATGTTGGAAACTGTTGAAAACTATTTTTCTTTTACACTAATCCAAATTCATCTCCTATTTCCTCATGATCTTCATCTTCTTCTTTTGTTTCTTTTTCCAAATTTGACATGCCTCTTTGCCTTGTTCAACAATTATGGTTTGGTCAGAATGTCATTCCAAAATTTTCCATATCGTTTTGCACTAATAGAGTTCTATCTATCCATTGTATTGATAAACAGATTCAATTTAACTATTTTAGTATCACATTTTTGTTGTTAGTGAAAATTGAAAGGATTAAAAAGTAA

mRNA sequence

ATGGACTGGACCAGAGGCCCCCCCGTCGGCCGCGGCTCCTCCGCCACCGTCTATATGGCTACAATCAACTCATCCGGCCAACAATTCGCCGTCAAATCCACCGACTTTGCATCATCGGCTCTGTTGCAGAAGGAACACGCTCTTCTCTCCAATTTGAGCTCCCCATACCTAATTAAGTCCCTAGGTTTCGACGTTGAAACCCAACCCTTCAATCCCCCGGTGTTCAATCTCTTCATGGAGTATTTGCCGGAGGGGACTCTGTGGGACGCGATTCAGAGCAACGGCGGTCGAATTGAGGAATCGATGATCCGAGTGTTTTCGCGGCAGATTCTCAAAGGATTGGACTACCTTCATGCTAATGGATTGGCACATTGCGATATCAAGAGCCGGAATCTGTTACTGAGTGATGGGGATTTGAAAATTGCCGATCTAGGTTGTGCGAAATTTGTGGATGGAATTTCCGGTAATGGAGTTGGGGCTTTTTCTGGTTCGCCGGCGTTCATGGCGCCGGAAGTTGCAAGAGGTGAAGAACAGGGATTTCCGGCCGATGTTTGGGCTTTTGGATGTACAGTCATTGAAATGGCTACTGGGGATCATCCATGGCCGGAAATAGAGGATCCGGTTTCGGCGTTGTATCGGATTGGATTTTCCGGCGAGTTACCAGAGATTCCGCGGTGGTTGTCGGAAATTGCGAGGGATTTTGTCGCCAAGTGTTTGATAAAAGACTCGAAAGAAAGATGGAGTGTGAAACGGCTTCTCGAGCATCCGTTTCTTCAAGAACTTGATTCTGAATCGGAAATTGAAGGGTCGAGGATGAAAATGGTGAATTCTTCTTCTCCGAGCTGTGTTTTGGATCAGAGGTTTTGGGATTCAATGGAAGATTTGGAGACTCCATGGAAGGTGACTCAACAAGCTTCTTTGGTGGATTGTCCAGCTGAGAGGATTAGAAGGTTGGCTGATAATTTCTCTTGCGATTTGGAATTGGAATGGGATGATGGAGACTGGGTTTTGATCAGAAACAATGGGGTCGGCGGTGGAAGCCATGGAAATGGATCTGTGAGCATAGATTTTTGTGCTACAGTTGAAGAAGAAGATGAAAATTTGAACTCGGAAATTGAAGAAAATTCTGTATTGTTCTGTTTAGTTGATGATGATTTTAGTGTTGTGAACAGTAGGAAAGGAGGATTACAAATGGAATTAAAGATGAAAATTGAAAGGATTAAAAAGTAA

Coding sequence (CDS)

ATGGACTGGACCAGAGGCCCCCCCGTCGGCCGCGGCTCCTCCGCCACCGTCTATATGGCTACAATCAACTCATCCGGCCAACAATTCGCCGTCAAATCCACCGACTTTGCATCATCGGCTCTGTTGCAGAAGGAACACGCTCTTCTCTCCAATTTGAGCTCCCCATACCTAATTAAGTCCCTAGGTTTCGACGTTGAAACCCAACCCTTCAATCCCCCGGTGTTCAATCTCTTCATGGAGTATTTGCCGGAGGGGACTCTGTGGGACGCGATTCAGAGCAACGGCGGTCGAATTGAGGAATCGATGATCCGAGTGTTTTCGCGGCAGATTCTCAAAGGATTGGACTACCTTCATGCTAATGGATTGGCACATTGCGATATCAAGAGCCGGAATCTGTTACTGAGTGATGGGGATTTGAAAATTGCCGATCTAGGTTGTGCGAAATTTGTGGATGGAATTTCCGGTAATGGAGTTGGGGCTTTTTCTGGTTCGCCGGCGTTCATGGCGCCGGAAGTTGCAAGAGGTGAAGAACAGGGATTTCCGGCCGATGTTTGGGCTTTTGGATGTACAGTCATTGAAATGGCTACTGGGGATCATCCATGGCCGGAAATAGAGGATCCGGTTTCGGCGTTGTATCGGATTGGATTTTCCGGCGAGTTACCAGAGATTCCGCGGTGGTTGTCGGAAATTGCGAGGGATTTTGTCGCCAAGTGTTTGATAAAAGACTCGAAAGAAAGATGGAGTGTGAAACGGCTTCTCGAGCATCCGTTTCTTCAAGAACTTGATTCTGAATCGGAAATTGAAGGGTCGAGGATGAAAATGGTGAATTCTTCTTCTCCGAGCTGTGTTTTGGATCAGAGGTTTTGGGATTCAATGGAAGATTTGGAGACTCCATGGAAGGTGACTCAACAAGCTTCTTTGGTGGATTGTCCAGCTGAGAGGATTAGAAGGTTGGCTGATAATTTCTCTTGCGATTTGGAATTGGAATGGGATGATGGAGACTGGGTTTTGATCAGAAACAATGGGGTCGGCGGTGGAAGCCATGGAAATGGATCTGTGAGCATAGATTTTTGTGCTACAGTTGAAGAAGAAGATGAAAATTTGAACTCGGAAATTGAAGAAAATTCTGTATTGTTCTGTTTAGTTGATGATGATTTTAGTGTTGTGAACAGTAGGAAAGGAGGATTACAAATGGAATTAAAGATGAAAATTGAAAGGATTAAAAAGTAA

Protein sequence

MDWTRGPPVGRGSSATVYMATINSSGQQFAVKSTDFASSALLQKEHALLSNLSSPYLIKSLGFDVETQPFNPPVFNLFMEYLPEGTLWDAIQSNGGRIEESMIRVFSRQILKGLDYLHANGLAHCDIKSRNLLLSDGDLKIADLGCAKFVDGISGNGVGAFSGSPAFMAPEVARGEEQGFPADVWAFGCTVIEMATGDHPWPEIEDPVSALYRIGFSGELPEIPRWLSEIARDFVAKCLIKDSKERWSVKRLLEHPFLQELDSESEIEGSRMKMVNSSSPSCVLDQRFWDSMEDLETPWKVTQQASLVDCPAERIRRLADNFSCDLELEWDDGDWVLIRNNGVGGGSHGNGSVSIDFCATVEEEDENLNSEIEENSVLFCLVDDDFSVVNSRKGGLQMELKMKIERIKK*
BLAST of Cucsa.007350.1 vs. Swiss-Prot
Match: NPK1_TOBAC (Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum GN=NPK1 PE=1 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 1.9e-42
Identity = 100/269 (37.17%), Postives = 151/269 (56.13%), Query Frame = 1

Query: 3   WTRGPPVGRGSSATVYMATINSSGQQFAVKSTDFASSAL-----------LQKEHALLSN 62
           W +G  +G G+   VYM     SG+  A+K    A +             L++E  LL N
Sbjct: 80  WRKGEMIGCGAFGRVYMGMNVDSGELLAIKEVSIAMNGASRERAQAHVRELEEEVNLLKN 139

Query: 63  LSSPYLIKSLGFDVETQPFNPPVFNLFMEYLPEGTLWDAIQSNGGRIEESMIRVFSRQIL 122
           LS P +++ LG   E    N     + +E++P G++  ++    G   ES+IR++++Q+L
Sbjct: 140 LSHPNIVRYLGTAREAGSLN-----ILLEFVPGGSI-SSLLGKFGSFPESVIRMYTKQLL 199

Query: 123 KGLDYLHANGLAHCDIKSRNLLLSD-GDLKIADLGCAK-FVDGISGNGVGAFSGSPAFMA 182
            GL+YLH NG+ H DIK  N+L+ + G +K+AD G +K  V+  +  G  +  G+P +MA
Sbjct: 200 LGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATMTGAKSMKGTPYWMA 259

Query: 183 PEVARGEEQGFPADVWAFGCTVIEMATGDHPWPEIEDPVSALYRIGFSGELPEIPRWLSE 242
           PEV       F AD+W+ GCT+IEMATG  PW +    V+AL+ IG +   P IP  LS 
Sbjct: 260 PEVILQTGHSFSADIWSVGCTIIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEHLSA 319

Query: 243 IARDFVAKCLIKDSKERWSVKRLLEHPFL 259
            ++DF+ KCL K+   R S   LL+HPF+
Sbjct: 320 ESKDFLLKCLQKEPHLRHSASNLLQHPFV 342

BLAST of Cucsa.007350.1 vs. Swiss-Prot
Match: M3K2_ARATH (Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana GN=ANP2 PE=2 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 5.4e-42
Identity = 103/291 (35.40%), Postives = 158/291 (54.30%), Query Frame = 1

Query: 3   WTRGPPVGRGSSATVYMATINSSGQQFAVKSTDFASSAL-----------LQKEHALLSN 62
           W +G  +GRG+  TVYM     SG+  AVK     S+             L++E  LL N
Sbjct: 68  WRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLITSNCASKEKTQAHIQELEEEVKLLKN 127

Query: 63  LSSPYLIKSLGFDVETQPFNPPVFNLFMEYLPEGTLWDAIQSNGGRIEESMIRVFSRQIL 122
           LS P +++ LG   E +       N+ +E++P G++   ++  G    ES++R ++ Q+L
Sbjct: 128 LSHPNIVRYLGTVREDE-----TLNILLEFVPGGSISSLLEKFGA-FPESVVRTYTNQLL 187

Query: 123 KGLDYLHANGLAHCDIKSRNLLLSD-GDLKIADLGCAKFVDGISG-NGVGAFSGSPAFMA 182
            GL+YLH + + H DIK  N+L+ + G +K+AD G +K V  ++  +G  +  G+P +MA
Sbjct: 188 LGLEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATISGAKSMKGTPYWMA 247

Query: 183 PEVARGEEQGFPADVWAFGCTVIEMATGDHPWPEIEDPVSALYRIGFSGELPEIPRWLSE 242
           PEV       F AD+W+ GCTVIEM TG  PW +    ++A++ IG +   P IP  +S 
Sbjct: 248 PEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKSHPPIPDNISS 307

Query: 243 IARDFVAKCLIKDSKERWSVKRLLEHPFLQELDSESEIEGSRMKMVNSSSP 281
            A DF+ KCL ++   R +   LL+HPF+     ES  +     M NS SP
Sbjct: 308 DANDFLLKCLQQEPNLRPTASELLKHPFVTGKQKESASKDLTSFMDNSCSP 352

BLAST of Cucsa.007350.1 vs. Swiss-Prot
Match: MKKA_DICDI (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum GN=mkkA PE=1 SV=2)

HSP 1 Score: 172.6 bits (436), Expect = 9.2e-42
Identity = 95/276 (34.42%), Postives = 156/276 (56.52%), Query Frame = 1

Query: 3   WTRGPPVGRGSSATVYMATINSSGQQFAVKS---TDFASSALLQ-------KEHALLSNL 62
           W +G  +GRG   +VY+     +G+ FAVK     D  S   L+       KE  ++ +L
Sbjct: 170 WQKGQILGRGGYGSVYLGLNKDTGELFAVKQLEIVDINSDPKLKNMILSFSKEIEVMRSL 229

Query: 63  SSPYLIKSLGFDVETQPFNPPVFNLFMEYLPEGTLWDAIQSNGGRIEESMIRVFSRQILK 122
               +++ LG  ++ Q F     ++F+EY+P G++  ++    G   E++I+V+++QIL+
Sbjct: 230 RHDNIVRYLGTSLD-QSF----LSVFLEYIPGGSI-SSLLGKFGAFSENVIKVYTKQILQ 289

Query: 123 GLDYLHANGLAHCDIKSRNLLL-SDGDLKIADLGCAKFVDGISGNGVGAFSGSPAFMAPE 182
           GL +LHAN + H DIK  N+L+ + G +K++D GC+K   GI      +  G+P +MAPE
Sbjct: 290 GLSFLHANSIIHRDIKGANILIDTKGIVKLSDFGCSKSFSGIVSQ-FKSMQGTPYWMAPE 349

Query: 183 VARGEEQGFPADVWAFGCTVIEMATGDHPWPEIEDPVSALYRIGFSGELPEIPRWLSEIA 242
           V +    G  +D+W+ GC ++EMAT   PW  I +  + +Y I  S  +P IP  +S+ A
Sbjct: 350 VIKQTGHGRSSDIWSLGCVIVEMATAQPPWSNITELAAVMYHIASSNSIPNIPSHMSQEA 409

Query: 243 RDFVAKCLIKDSKERWSVKRLLEHPFLQELDSESEI 268
            DF+  C  +D KER    +LL+HPF+  LD   ++
Sbjct: 410 FDFLNLCFKRDPKERPDANQLLKHPFIMNLDDNIQL 438

BLAST of Cucsa.007350.1 vs. Swiss-Prot
Match: ANP1_ARATH (Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana GN=ANP1 PE=1 SV=2)

HSP 1 Score: 171.0 bits (432), Expect = 2.7e-41
Identity = 106/293 (36.18%), Postives = 160/293 (54.61%), Query Frame = 1

Query: 1   MDWTRGPPVGRGSSATVYMATINSSGQQFAVK----STDFASSAL-------LQKEHALL 60
           + W +G  +GRG+  TVYM     SG+  AVK    + +FAS          L++E  LL
Sbjct: 67  ISWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQELEEEVKLL 126

Query: 61  SNLSSPYLIKSLGFDVETQPFNPPVFNLFMEYLPEGTLWDAIQSNGGRIEESMIRVFSRQ 120
            NLS P +++ LG   E         N+ +E++P G++  ++    G   ES++R ++RQ
Sbjct: 127 KNLSHPNIVRYLGTVREDD-----TLNILLEFVPGGSI-SSLLEKFGPFPESVVRTYTRQ 186

Query: 121 ILKGLDYLHANGLAHCDIKSRNLLLSD-GDLKIADLGCAKFVDGISG-NGVGAFSGSPAF 180
           +L GL+YLH + + H DIK  N+L+ + G +K+AD G +K V  ++   G  +  G+P +
Sbjct: 187 LLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTPYW 246

Query: 181 MAPEVARGEEQGFPADVWAFGCTVIEMATGDHPWPEIEDPVSALYRIGFSGELPEIPRWL 240
           MAPEV       F AD+W+ GCTVIEM TG  PW +    V+A++ IG +   P IP  L
Sbjct: 247 MAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPDTL 306

Query: 241 SEIARDFVAKCLIKDSKERWSVKRLLEHPFLQELDSESEIEGSRMKMVNSSSP 281
           S  A+DF+ KCL +    R +   LL+HPF+     ES        + N S+P
Sbjct: 307 SSDAKDFLLKCLQEVPNLRPTASELLKHPFVMGKHKESASTDLGSVLNNLSTP 353

BLAST of Cucsa.007350.1 vs. Swiss-Prot
Match: M3K3_ARATH (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana GN=ANP3 PE=1 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 1.0e-40
Identity = 101/269 (37.55%), Postives = 150/269 (55.76%), Query Frame = 1

Query: 3   WTRGPPVGRGSSATVYMATINSSGQQFAVKSTDFASSAL-----------LQKEHALLSN 62
           W +G  +G G+   VYM     SG+  A+K    A S+            L++E  LL N
Sbjct: 68  WRKGELIGCGAFGRVYMGMNLDSGELLAIKQVLIAPSSASKEKTQGHIRELEEEVQLLKN 127

Query: 63  LSSPYLIKSLGFDVETQPFNPPVFNLFMEYLPEGTLWDAIQSNGGRIEESMIRVFSRQIL 122
           LS P +++ LG   E+   N     + ME++P G++   ++  G    E +I ++++Q+L
Sbjct: 128 LSHPNIVRYLGTVRESDSLN-----ILMEFVPGGSISSLLEKFGS-FPEPVIIMYTKQLL 187

Query: 123 KGLDYLHANGLAHCDIKSRNLLLSD-GDLKIADLGCAK-FVDGISGNGVGAFSGSPAFMA 182
            GL+YLH NG+ H DIK  N+L+ + G +++AD G +K  V+  + NG  +  G+P +MA
Sbjct: 188 LGLEYLHNNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATVNGAKSMKGTPYWMA 247

Query: 183 PEVARGEEQGFPADVWAFGCTVIEMATGDHPWPEIEDPVSALYRIGFSGELPEIPRWLSE 242
           PEV       F AD+W+ GCTVIEMATG  PW E     +A+  IG +   P IP  LS 
Sbjct: 248 PEVILQTGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTKAHPPIPEDLSP 307

Query: 243 IARDFVAKCLIKDSKERWSVKRLLEHPFL 259
            A+DF+ KCL K+   R S   LL+HPF+
Sbjct: 308 EAKDFLMKCLHKEPSLRLSATELLQHPFV 330

BLAST of Cucsa.007350.1 vs. TrEMBL
Match: W9RB46_9ROSA (Mitogen-activated protein kinase kinase kinase A OS=Morus notabilis GN=L484_008068 PE=3 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 6.7e-100
Identity = 194/375 (51.73%), Postives = 264/375 (70.40%), Query Frame = 1

Query: 1   MDWTRGPPVGRGSSATVYMATINSSGQQFAVKSTDFASSALLQKEHALLSNLSSPYLIKS 60
           M+WTRGP +GRG++ATV++AT   SG+ FAVKS +   +  L+KE + LS LSS  ++K 
Sbjct: 1   MEWTRGPTIGRGATATVHLATDTRSGEPFAVKSAELCRAVFLRKEQSFLSKLSSSRIVKY 60

Query: 61  LGFDVETQPFNPPVFNLFMEYLPEGTLWDAIQSNGGRIEESMIRVFSRQILKGLDYLHAN 120
           LGFDV T+  + P++NLF+EYLP GTL+DAI+S  GR++ES+I+++++QIL+GLD++H N
Sbjct: 61  LGFDVTTEN-DVPMYNLFIEYLPNGTLFDAIRSREGRLDESLIKIYTQQILQGLDHIHEN 120

Query: 121 GLAHCDIKSRNLLLSDGDLKIADLGCAKFV-DGISGNG--VGAFSGSPAFMAPEVARGEE 180
           GL HCDIKS+N+L+ +  +KIADLGCAKFV +   G+   V  FSG+PAFMAPEVARGE+
Sbjct: 121 GLVHCDIKSQNMLIGEEGIKIADLGCAKFVGEAEFGDSPLVLQFSGTPAFMAPEVARGEK 180

Query: 181 QGFPADVWAFGCTVIEMATGDHPWPEIEDPVSALYRIGFSGELPEIPRWLSEIARDFVAK 240
           QG  ADVWA GCTVIEMATG  PWPE++DPVSALYRIG+S  +PEIPRW SE  +DF++K
Sbjct: 181 QGLEADVWALGCTVIEMATGRGPWPEMDDPVSALYRIGYSDRVPEIPRWFSEKGKDFLSK 240

Query: 241 CLIKDSKERWSVKRLLEHPFLQELDSESEIEGSRMKMVNSSSPSCVLDQRFWDSMEDLET 300
           CL +D +ERW+V  LLEHPFLQ     SE+   +      SSPS VLD+  WDS E  E+
Sbjct: 241 CLKRDWRERWTVNELLEHPFLQ----VSELRSEQQVKAKMSSPSSVLDKDLWDSFEAFES 300

Query: 301 PWKVTQQASLVDCPAERIRRLADNFSCDLE--LEW--DDGDWVLIRNNGVGGGSHGN--- 360
                Q+AS ++   ERI+ L  +  C ++   +W  D+ +W  IR+N +    H     
Sbjct: 301 --HQDQEASSLNSAEERIKMLIRSAFCSVQNLPDWSCDEENWFTIRSNSIEEPIHEEELV 360

Query: 361 -GSVSIDFCATVEEE 365
            GS++ D   + +EE
Sbjct: 361 YGSITFDEELSFDEE 368

BLAST of Cucsa.007350.1 vs. TrEMBL
Match: V4WGB5_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10010150mg PE=3 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 2.6e-99
Identity = 188/356 (52.81%), Postives = 246/356 (69.10%), Query Frame = 1

Query: 1   MDWTRGPPVGRGSSATVYMATINSSGQQFAVKSTDFASSALLQKEHALLSNLSSPYLIKS 60
           M+WTRGP +GRGS+ATVY+A + SSG+  AVKST+ + S  LQ+E   LS +SSPY++  
Sbjct: 1   MEWTRGPIIGRGSTATVYLANLASSGKFIAVKSTELSQSMFLQREQCFLSKISSPYVVDY 60

Query: 61  LGFDVETQPFNPPVFNLFMEYLPEGTLWDAIQSNGGRIEESMIRVFSRQILKGLDYLHAN 120
           LGF+V T   N P +NL+MEY+P GTL+D I+ +GGR++E MI++++ QIL+GL++LH N
Sbjct: 61  LGFNV-TNENNTPTYNLYMEYMPRGTLYDEIRRHGGRLDEQMIKLYAWQILQGLNHLHMN 120

Query: 121 GLAHCDIKSRNLLLSDGDLKIADLGCAKFVDGISGNGV---GAFSGSPAFMAPEVARGEE 180
           G+AHCDIKS N+L+ D   KIADLGCAK+V  I  N      AFSG+P FMAPEVAR EE
Sbjct: 121 GIAHCDIKSNNVLIGDDGAKIADLGCAKYVGEIDSNAALATSAFSGTPVFMAPEVARREE 180

Query: 181 QGFPADVWAFGCTVIEMATGDHPWPEIEDPVSALYRIGFSGELPEIPRWLSEIARDFVAK 240
           QG  AD+WA GCT++EMATG +PWPE+ DPVSALYRIGFSGE PEIPRWLS  A+DF+ K
Sbjct: 181 QGLAADIWAVGCTIVEMATGTNPWPELNDPVSALYRIGFSGETPEIPRWLSMEAKDFLCK 240

Query: 241 CLIKDSKERWSVKRLLEHPFL--QELDSESEIEGSRMKMVNSSSPSCVLDQRFWDSMEDL 300
           CL KD KER + K LLEHPF+   E D   ++E   M     SSP  VL+  FWDS+E+ 
Sbjct: 241 CLTKDPKERLTAKELLEHPFICGVEFDHFKDVEKFIM-----SSPCTVLNHGFWDSLEEA 300

Query: 301 ETPWKVTQQAS-LVDCPAERIRRLADNFSCDLEL------EWD-DGDWVLIRNNGV 344
           E+   ++ +AS         ++R+     C L         W  D DW+ +R+NG+
Sbjct: 301 ESSENLSPKASPSTSSSNSAVKRIQQLIGCALPSLGSSVPNWSLDEDWITVRSNGI 350

BLAST of Cucsa.007350.1 vs. TrEMBL
Match: A0A068V557_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00015855001 PE=3 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 2.6e-99
Identity = 203/416 (48.80%), Postives = 275/416 (66.11%), Query Frame = 1

Query: 3   WTRGPPVGRGSSATVYMATINSSGQQFAVKSTDFASSALLQKEHALLSNLSSPYLIKSLG 62
           WTRGP +GRGSSATV +AT  +SG+  AVKS D + S++LQKE +L+S LSSPY++K +G
Sbjct: 4   WTRGPIIGRGSSATVSLAT-TASGELLAVKSADLSCSSVLQKEESLISELSSPYIVKYVG 63

Query: 63  FDVETQPFNPPVFNLFMEYLPEGTLWDAIQSNGGRIEESMIRVFSRQILKGLDYLHANGL 122
            DV T+  + P++NL MEY+P GTL D I+  GG +EESMI+VF+ QIL+GLDYLH NG+
Sbjct: 64  CDVTTEN-DKPLYNLLMEYVPGGTLSDQIRKQGGPLEESMIQVFAHQILQGLDYLHLNGI 123

Query: 123 AHCDIKSRNLLLSDGDLKIADLGCAKFVDGISGNGVGAFS---GSPAFMAPEVARGEEQG 182
            HCDIK +N+L+     KI DLGCA+ V+   GNG+   S   G+P FMAPEVARGEEQG
Sbjct: 124 VHCDIKGQNVLIGRDGAKIGDLGCARVVE--EGNGMAGKSVICGTPMFMAPEVARGEEQG 183

Query: 183 FPADVWAFGCTVIEMATGDHPWPEIEDPVSALYRIGFSGELPEIPRWLSEIARDFVAKCL 242
           F AD+WA GCT+IEMAT ++PWP++EDPVSALYRIG+SG++PE P WLS+ A++F++KCL
Sbjct: 184 FAADIWALGCTIIEMATANNPWPDMEDPVSALYRIGYSGDVPEFPCWLSDGAKEFLSKCL 243

Query: 243 IKDSKERWSVKRLLEHPFLQELDSESEIEGSRMKMVNSSSPSCVLDQRFWDSMEDLETPW 302
            +D+KERW+ + LL+HPF   +  E E E  R      +SP+ VLDQ FWDSME  E+  
Sbjct: 244 KRDAKERWTARELLQHPFFHGVGEEKEAEFVR------NSPTTVLDQGFWDSMEVAESSL 303

Query: 303 KVTQQ-ASLVDCPAERIRRLADN-FSCDLEL-EW-DDGDWVLIR-----------NNGVG 362
           + +    S +  PA+RI+ L  N FS +     W +DGDWV +R                
Sbjct: 304 QDSSHIVSSLGSPADRIKNLMGNLFSSNANFPNWSEDGDWVTVRGTDSQEIQPVSQQNEN 363

Query: 363 GGSHGNGSVSIDFCATVEEEDENLNSEIEENSVLFCLVDDDFSVVNSRKGGLQMEL 401
              H +      F  +++ E+E L S I  + +L   + D+ S V +  GGL + L
Sbjct: 364 SEVHDSQLAMDPFLYSIDLEEE-LESSIVIDDLLINCLVDEISNVGNVSGGLNLTL 408

BLAST of Cucsa.007350.1 vs. TrEMBL
Match: B9SVA5_RICCO (Mitogen-activated protein kinase kinase kinase, putative OS=Ricinus communis GN=RCOM_1303640 PE=3 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 7.4e-99
Identity = 193/354 (54.52%), Postives = 255/354 (72.03%), Query Frame = 1

Query: 1   MDWTRGPPVGRGSSATVYMATINSSGQQFAVKSTDFASSALLQKEHALLSNLSSPYLIKS 60
           M+WTRGP +GRGS+ATV +AT   SG+ FA+KST+ + S  LQKE + LS ++SP+++K 
Sbjct: 1   MEWTRGPVIGRGSTATVSLATSVFSGELFALKSTELSKSMFLQKEQSFLSKINSPHIVKY 60

Query: 61  LGFDVETQPFNPPVFNLFMEYLPEGTLWDAIQSNGGRIEESMIRVFSRQILKGLDYLHAN 120
           +G+D+ T      ++N+ +EY+P GTL D I  +GG+++E MI  ++R IL+GLDYLH N
Sbjct: 61  IGYDI-TNENTQSLYNICLEYVPGGTLHDVILRHGGQLDEPMIGSYTRNILQGLDYLHRN 120

Query: 121 -GLAHCDIKSRNLLLSDGDLKIADLGCAKFVDGISGNGVG---AFSGSPAFMAPEVARGE 180
            GL HCDIKS+N+L+S    KIAD GCAKFV+ ++ NG G   AFSG+PAFM+PEVARGE
Sbjct: 121 IGLVHCDIKSKNVLISKDGAKIADFGCAKFVEQVARNGGGDASAFSGTPAFMSPEVARGE 180

Query: 181 EQGFPADVWAFGCTVIEMATGDHPWPEI---EDPVSALYRIGFSGELPEIPRWLSEIARD 240
           EQGFPAD+WA GCTVIEMATG  PW EI   +DP+S LYRIGFS E PE P WLSE  +D
Sbjct: 181 EQGFPADIWAVGCTVIEMATGSIPWAEIMKNDDPLSVLYRIGFSSEAPEFPSWLSEKGKD 240

Query: 241 FVAKCLIKDSKERWSVKRLLEHPFLQELDSESEIEGSRMKMVN-SSSPSCVLDQRFWDSM 300
           F++KCL +DSKERW+ K LL+HPFL EL+ E       +K V+  SSPSCVLDQ FW+SM
Sbjct: 241 FLSKCLRRDSKERWTAKELLDHPFLGELELE-------LKDVDYQSSPSCVLDQDFWNSM 300

Query: 301 EDLETPWKVTQQ--ASLVDCPAERIRRLADNFS---CDLELEWDDGDWVLIRNN 342
           +  E+P  +T +  +SL + PAERI RL  + S    D+     + DW+ +R+N
Sbjct: 301 DAFESPQDLTPERFSSLNNSPAERINRLIGSNSLLVADVPNWSLEEDWITVRSN 346

BLAST of Cucsa.007350.1 vs. TrEMBL
Match: A0A067FF28_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g045508mg PE=3 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 1.3e-98
Identity = 187/356 (52.53%), Postives = 245/356 (68.82%), Query Frame = 1

Query: 1   MDWTRGPPVGRGSSATVYMATINSSGQQFAVKSTDFASSALLQKEHALLSNLSSPYLIKS 60
           M+WTRGP +GRGS+ATVY+A + SSG+  AVKST+ + S  LQ+E   LS +SSPY++  
Sbjct: 1   MEWTRGPIIGRGSTATVYLANLASSGKFIAVKSTELSRSMFLQREQCFLSKISSPYVVDY 60

Query: 61  LGFDVETQPFNPPVFNLFMEYLPEGTLWDAIQSNGGRIEESMIRVFSRQILKGLDYLHAN 120
           LGF+V T   N P +NL+MEY+P GTL+D I+ +GGR++E MI++++ QIL+GL++LH N
Sbjct: 61  LGFNV-TNENNTPTYNLYMEYMPRGTLYDEIRRHGGRLDEQMIKLYAWQILQGLNHLHMN 120

Query: 121 GLAHCDIKSRNLLLSDGDLKIADLGCAKFVDGISGNGV---GAFSGSPAFMAPEVARGEE 180
           G+AHCDIKS N+L+ D   KIADLGCAK+V  I  N      AFSG+P FM PEVAR EE
Sbjct: 121 GIAHCDIKSNNVLIGDDGAKIADLGCAKYVGEIDSNAALATSAFSGTPVFMPPEVARREE 180

Query: 181 QGFPADVWAFGCTVIEMATGDHPWPEIEDPVSALYRIGFSGELPEIPRWLSEIARDFVAK 240
           QG  AD+WA GCT++EMATG +PWPE+ DPVSALYRIGFSGE PEIPRWLS  A+DF+ K
Sbjct: 181 QGLAADIWAVGCTIVEMATGTNPWPELNDPVSALYRIGFSGETPEIPRWLSMEAKDFLCK 240

Query: 241 CLIKDSKERWSVKRLLEHPFL--QELDSESEIEGSRMKMVNSSSPSCVLDQRFWDSMEDL 300
           CL KD KER + K LLEHPF+   E D   ++E   M     SSP  VL+  FWDS+E+ 
Sbjct: 241 CLTKDPKERLTAKELLEHPFICGVEFDHFKDVEKFIM-----SSPCTVLNHGFWDSLEEA 300

Query: 301 ETPWKVTQQAS-LVDCPAERIRRLADNFSCDLEL------EWD-DGDWVLIRNNGV 344
           E+   ++ +AS         ++R+     C L         W  D DW+ +R+NG+
Sbjct: 301 ESSENLSPKASPSTSSSNSAVKRIQQLIGCALPSLGSSVPNWSLDEDWITVRSNGI 350

BLAST of Cucsa.007350.1 vs. TAIR10
Match: AT4G26890.1 (AT4G26890.1 mitogen-activated protein kinase kinase kinase 16)

HSP 1 Score: 346.7 bits (888), Expect = 2.0e-95
Identity = 186/351 (52.99%), Postives = 247/351 (70.37%), Query Frame = 1

Query: 1   MDWTRGPPVGRGSSATVYMATINSSGQQFAVKSTDFASSALLQKEHALLSNLSSPYLIKS 60
           ++WTRGP +GRGS+ATV +A I+SSG+ FAVKS D +SS+LLQKE ++LS LSSP+++K 
Sbjct: 3   INWTRGPIIGRGSTATVSIA-ISSSGELFAVKSADLSSSSLLQKEQSILSTLSSPHMVKY 62

Query: 61  LGFDVETQPFNPPVFNLFMEYLPEGTLWDAIQSNGGRIEESMIRVFSRQILKGLDYLHAN 120
           +G  + T+  N  V+N+ MEY+  G L D I+++GG++ E  IR ++RQIL GL YLH  
Sbjct: 63  IGTGL-TRESNGLVYNILMEYVSGGNLHDLIKNSGGKLPEPEIRSYTRQILNGLVYLHER 122

Query: 121 GLAHCDIKSRNLLLSD-GDLKIADLGCAKFVDGISGNGVGAFSGSPAFMAPEVARGEEQG 180
           G+ HCD+KS N+L+ + G LKIAD+GCAK VD         FSG+PAFMAPEVARGEEQ 
Sbjct: 123 GIVHCDLKSHNVLVEENGVLKIADMGCAKSVDK------SEFSGTPAFMAPEVARGEEQR 182

Query: 181 FPADVWAFGCTVIEMATGDHPWPEIEDPVSALYRIGFSGELPEIPRWLSEIARDFVAKCL 240
           FPADVWA GCT+IEM TG  PWPE+ D V+A+Y+IGFSGE P IP W+S+ A+DF+  CL
Sbjct: 183 FPADVWALGCTMIEMMTGSSPWPELNDVVAAMYKIGFSGESPAIPAWISDKAKDFLKNCL 242

Query: 241 IKDSKERWSVKRLLEHPFLQELDSESEIEGSRMKMVNSSSPSCVLDQRFWDSME------ 300
            +D K+RW+V+ LL+HPF   LD + E + S      +SSPS VLDQRFWDS E      
Sbjct: 243 KEDQKQRWTVEELLKHPF---LDDDEESQTSDCLKNKTSSPSTVLDQRFWDSCESSKSHL 302

Query: 301 ---DLETPWKVTQQASLVDCPAERIRRLA-DNFSCDLELEW-DDGDWVLIR 340
              D E P+   + +  +D PA+RI +LA D FS  L+ +  DDG W+ +R
Sbjct: 303 VSIDHEDPF--AEYSESLDSPADRIEKLAGDEFSSLLDWDTEDDGGWIQVR 340

BLAST of Cucsa.007350.1 vs. TAIR10
Match: AT5G55090.1 (AT5G55090.1 mitogen-activated protein kinase kinase kinase 15)

HSP 1 Score: 342.4 bits (877), Expect = 3.8e-94
Identity = 181/349 (51.86%), Postives = 244/349 (69.91%), Query Frame = 1

Query: 2   DWTRGPPVGRGSSATVYMATINSSGQQFAVKSTDFASSALLQKEHALLSNLSSPYLIKSL 61
           +W RGP +GRGS+ATV +  I +SG  FAVKS +F+SSA LQ+E ++LS LSSPY++K +
Sbjct: 5   NWIRGPIIGRGSTATVSLG-ITNSGDFFAVKSAEFSSSAFLQREQSILSKLSSPYIVKYI 64

Query: 62  GFDVETQPFNPPVFNLFMEYLPEGTLWDAIQSNGGRIEESMIRVFSRQILKGLDYLHANG 121
           G +V T+  +  ++NL MEY+  G+L D I+++GG++ E +IR ++RQILKGL YLH  G
Sbjct: 65  GSNV-TKENDKLMYNLLMEYVSGGSLHDLIKNSGGKLPEPLIRSYTRQILKGLMYLHDQG 124

Query: 122 LAHCDIKSRNLLLSDGDLKIADLGCAKFVDGISGNGVGAFSGSPAFMAPEVARGEEQGFP 181
           + HCD+KS+N+++     KI DLGCAK V+    N    FSG+PAFM+PEVARGEEQ FP
Sbjct: 125 IVHCDVKSQNVMIGGEIAKIVDLGCAKTVEE---NENLEFSGTPAFMSPEVARGEEQSFP 184

Query: 182 ADVWAFGCTVIEMATGDHPWPEIEDPVSALYRIGFSGELPEIPRWLSEIARDFVAKCLIK 241
           ADVWA GCTVIEMATG  PWPE+ D V+A+Y+IGF+GE P IP WLSE  +DF+ KCL K
Sbjct: 185 ADVWALGCTVIEMATGSSPWPELNDVVAAIYKIGFTGESPVIPVWLSEKGQDFLRKCLRK 244

Query: 242 DSKERWSVKRLLEHPFLQELDSESEIEGSRMKMVNSSSPSCVLDQRFWD---------SM 301
           D K+RW+V+ LL+HPFL E D++S+  G+    +NSSSPS VLDQRFWD           
Sbjct: 245 DPKQRWTVEELLQHPFLDEEDNDSDQTGN---CLNSSSPSTVLDQRFWDLCETSRSRFIK 304

Query: 302 EDLETPWKVTQQASLVD--CPAERIRRLADNFSCDLELEWDDGDWVLIR 340
           ED E P+  +      D   P +RI++LA + S   E +W+   W+ +R
Sbjct: 305 EDHEDPFANSTNFLWDDDSLPGDRIKKLAGDESSG-EPDWETNGWIEVR 344

BLAST of Cucsa.007350.1 vs. TAIR10
Match: AT2G32510.1 (AT2G32510.1 mitogen-activated protein kinase kinase kinase 17)

HSP 1 Score: 300.1 bits (767), Expect = 2.1e-81
Identity = 161/356 (45.22%), Postives = 232/356 (65.17%), Query Frame = 1

Query: 1   MDWTRGPPVGRGSSATVYMATINSSGQQFAVKSTDFASSALLQKEHALLSNLSSPYLIKS 60
           M+WTRG  +GRGS+ATVY A  ++S +  AVKS++   S  LQ+E  +LS+LSSPY+I  
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNSDEILAVKSSEVHRSEFLQREAKILSSLSSPYVIGY 60

Query: 61  LGFDVETQPFNPPVFNLFMEYLPEGTLWDAIQSNGGRIEESMIRVFSRQILKGLDYLHAN 120
            G + + +     ++NL MEY P GTL DA   +GGR++E+ +  ++R ILKGL+Y+H+ 
Sbjct: 61  RGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGLEYIHSK 120

Query: 121 GLAHCDIKSRNLLLSD-GDLKIADLGCAKFVDGISGNGVGAFSGSPAFMAPEVARGEEQG 180
           G+ HCD+K  N+++S+ G+ KIAD GCAK VD +  + V    G+PAFMAPEVARGE+QG
Sbjct: 121 GIVHCDVKGSNVVISEKGEAKIADFGCAKRVDPVFESPV---MGTPAFMAPEVARGEKQG 180

Query: 181 FPADVWAFGCTVIEMATGDHPWPEI---EDPVSALYRIGFSGELPEIPRWLSEIARDFVA 240
             +D+WA GCT+IEM TG  PW +    EDPVS LYR+G+S E PE+P  L+E A+DF+ 
Sbjct: 181 KESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCLLAEEAKDFLE 240

Query: 241 KCLIKDSKERWSVKRLLEHPFLQELDSESEIEGSRMKMVNSSSPSCVLDQRFWDSM---- 300
           KCL +++ ERW+  +LL HPF   L ++ +IE   +  + S+SP+ V DQ FW S+    
Sbjct: 241 KCLKREANERWTATQLLNHPF---LTTKPDIEPVLVPGLISNSPTSVTDQTFWRSVEEEE 300

Query: 301 ----EDLETPWKVTQQASLVDCPAERIRRL-----ADNFSCDLELEWDDGDWVLIR 340
               E+++   +   + SL  C +ERI RL      D   CD+E     GDW+++R
Sbjct: 301 EEETEEIQKDSRDLDRLSLWGCYSERIGRLKCVGGLDGTRCDME----GGDWIMVR 346

BLAST of Cucsa.007350.1 vs. TAIR10
Match: AT1G05100.1 (AT1G05100.1 mitogen-activated protein kinase kinase kinase 18)

HSP 1 Score: 285.8 bits (730), Expect = 4.2e-77
Identity = 163/352 (46.31%), Postives = 227/352 (64.49%), Query Frame = 1

Query: 1   MDWTRGPPVGRGSSATVYMATINSSGQQFAVKSTDFASSALLQKEHALLSNLSSPYLIKS 60
           M+WTRG  +GRGS+ATV  AT + SG+  AVKS +F  S  LQ+E  +LS+L+SPY+I  
Sbjct: 1   MNWTRGKTLGRGSTATVSAATCHESGETLAVKSAEFHRSEFLQREAKILSSLNSPYVIGY 60

Query: 61  LGFDVETQPFN----PPVFNLFMEYLPEGTLWDAIQSNGGRIEESMIRVFSRQILKGLDY 120
            G ++  +PF+       ++L MEY P GTL D    NGG I+E+ +  ++RQIL GL+Y
Sbjct: 61  RGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGGFIDEARVVKYTRQILLGLEY 120

Query: 121 LH-ANGLAHCDIKSRNLLLSD-GDLKIADLGCAKFVDGISGNGVGAFSGSPAFMAPEVAR 180
           +H + G+AHCDIK  N+L+ + G+ KIAD GCAK+V+      V    G+PAFMAPE AR
Sbjct: 121 IHNSKGIAHCDIKGSNVLVGENGEAKIADFGCAKWVEPEITEPV---RGTPAFMAPEAAR 180

Query: 181 GEEQGFPADVWAFGCTVIEMATGDHPW--PEIEDPVSALYRIGFSGELPEIPRWLSEIAR 240
           GE QG  +D+WA GCTVIEM TG  PW   +  DPVS LYR+G+ GELPE+P  L+E A+
Sbjct: 181 GERQGKESDIWAVGCTVIEMVTGSQPWIGADFTDPVSVLYRVGYLGELPELPCSLTEQAK 240

Query: 241 DFVAKCLIKDSKERWSVKRLLEHPFLQELDSESEIEGSRMKMVNSSSPSCVLDQRFWDSM 300
           DF+ KCL K++ ERW+  +LL HPFL  ++ E E+    +  + ++SP+ V DQ FW S+
Sbjct: 241 DFLGKCLKKEATERWTASQLLNHPFL--VNKEPEL----VTGLVTNSPTSVTDQMFWRSV 300

Query: 301 EDLETPWKVTQQASLVDC-PAERIRRLADNFSCDLELEWD-DG-DWVLIRNN 342
           E+  +      ++S  +C   ERI  L+      +E  WD DG DW+ +R N
Sbjct: 301 EEEVS----EDRSSWWECHEDERIGVLSWIGHVVVESTWDLDGEDWITVRRN 339

BLAST of Cucsa.007350.1 vs. TAIR10
Match: AT4G36950.1 (AT4G36950.1 mitogen-activated protein kinase kinase kinase 21)

HSP 1 Score: 200.3 bits (508), Expect = 2.3e-51
Identity = 135/350 (38.57%), Postives = 185/350 (52.86%), Query Frame = 1

Query: 1   MDWTRGPPVGRGSSATVYMATINSSGQQ-----FAVKSTDFASSALLQKEHALLSNLSSP 60
           M+W R   +G GS +TV +AT + S  +      AVKS+    SA L+ E  +L +L   
Sbjct: 1   MEWIRRETIGHGSFSTVSLATTSGSSSKAFPSLMAVKSSGVVCSAALRNERDVLDDLGDC 60

Query: 61  YLIKSLGFDVETQPFNPPVFNLFMEYLPEGTLWDAIQSNGGRIEESMIRVFSRQILKGLD 120
             I     +  T      ++NLF+EY   G+L D I+S+G  + E  +R F+R I+KGL 
Sbjct: 61  SEIVRCFGEGRTVENGEEIYNLFLEYASGGSLADRIKSSGEALPEFEVRRFTRSIVKGLC 120

Query: 121 YLHANGLAHCDIKSRNLLL-SDGDLKIADLGCAKFVDGISGNGVGAFSGSPAFMAPEVAR 180
           ++H NG  HCDIK  N+L+  DGD+KI+D G AK     SG       G+P +MAPE   
Sbjct: 121 HIHGNGFTHCDIKLENVLVFGDGDVKISDFGLAK---RRSGEVCVEIRGTPLYMAPESVN 180

Query: 181 GEEQGFPADVWAFGCTVIEMATGDHPWPEIEDPV-----SALYRIGFSGELPEIPRWLSE 240
             E   PAD+WA GC+V+EM++G   W  +ED V     S L RIG   E+P IP  LSE
Sbjct: 181 HGEFESPADIWALGCSVVEMSSGKTAWC-LEDGVMNNVMSLLVRIGSGDEVPRIPVELSE 240

Query: 241 IARDFVAKCLIKDSKERWSVKRLLEHPFLQELDSESEIEGSRMKMVNSSSPSCVLDQRFW 300
             +DFV+KC +K++ ERW+ + LL+HPFL  +D ES  E        S SP    D   W
Sbjct: 241 EGKDFVSKCFVKNAAERWTAEMLLDHPFL-AVDDESGEEDEAC----SVSPRNPFDFPGW 300

Query: 301 DSMEDLETPWKVTQQASLVDCPAERIRRLADNFSCDLELEWDDGDWVLIR 340
           +S++            SLV  P ERI  L      D  +     DWV +R
Sbjct: 301 NSVQSPVN--DSVMFGSLVGSPEERISGLVSEKVPDWSVSC---DWVNVR 336

BLAST of Cucsa.007350.1 vs. NCBI nr
Match: gi|778730258|ref|XP_011659740.1| (PREDICTED: mitogen-activated protein kinase kinase kinase 2, partial [Cucumis sativus])

HSP 1 Score: 819.7 bits (2116), Expect = 2.3e-234
Identity = 401/401 (100.00%), Postives = 401/401 (100.00%), Query Frame = 1

Query: 1   MDWTRGPPVGRGSSATVYMATINSSGQQFAVKSTDFASSALLQKEHALLSNLSSPYLIKS 60
           MDWTRGPPVGRGSSATVYMATINSSGQQFAVKSTDFASSALLQKEHALLSNLSSPYLIKS
Sbjct: 1   MDWTRGPPVGRGSSATVYMATINSSGQQFAVKSTDFASSALLQKEHALLSNLSSPYLIKS 60

Query: 61  LGFDVETQPFNPPVFNLFMEYLPEGTLWDAIQSNGGRIEESMIRVFSRQILKGLDYLHAN 120
           LGFDVETQPFNPPVFNLFMEYLPEGTLWDAIQSNGGRIEESMIRVFSRQILKGLDYLHAN
Sbjct: 61  LGFDVETQPFNPPVFNLFMEYLPEGTLWDAIQSNGGRIEESMIRVFSRQILKGLDYLHAN 120

Query: 121 GLAHCDIKSRNLLLSDGDLKIADLGCAKFVDGISGNGVGAFSGSPAFMAPEVARGEEQGF 180
           GLAHCDIKSRNLLLSDGDLKIADLGCAKFVDGISGNGVGAFSGSPAFMAPEVARGEEQGF
Sbjct: 121 GLAHCDIKSRNLLLSDGDLKIADLGCAKFVDGISGNGVGAFSGSPAFMAPEVARGEEQGF 180

Query: 181 PADVWAFGCTVIEMATGDHPWPEIEDPVSALYRIGFSGELPEIPRWLSEIARDFVAKCLI 240
           PADVWAFGCTVIEMATGDHPWPEIEDPVSALYRIGFSGELPEIPRWLSEIARDFVAKCLI
Sbjct: 181 PADVWAFGCTVIEMATGDHPWPEIEDPVSALYRIGFSGELPEIPRWLSEIARDFVAKCLI 240

Query: 241 KDSKERWSVKRLLEHPFLQELDSESEIEGSRMKMVNSSSPSCVLDQRFWDSMEDLETPWK 300
           KDSKERWSVKRLLEHPFLQELDSESEIEGSRMKMVNSSSPSCVLDQRFWDSMEDLETPWK
Sbjct: 241 KDSKERWSVKRLLEHPFLQELDSESEIEGSRMKMVNSSSPSCVLDQRFWDSMEDLETPWK 300

Query: 301 VTQQASLVDCPAERIRRLADNFSCDLELEWDDGDWVLIRNNGVGGGSHGNGSVSIDFCAT 360
           VTQQASLVDCPAERIRRLADNFSCDLELEWDDGDWVLIRNNGVGGGSHGNGSVSIDFCAT
Sbjct: 301 VTQQASLVDCPAERIRRLADNFSCDLELEWDDGDWVLIRNNGVGGGSHGNGSVSIDFCAT 360

Query: 361 VEEEDENLNSEIEENSVLFCLVDDDFSVVNSRKGGLQMELK 402
           VEEEDENLNSEIEENSVLFCLVDDDFSVVNSRKGGLQMELK
Sbjct: 361 VEEEDENLNSEIEENSVLFCLVDDDFSVVNSRKGGLQMELK 401

BLAST of Cucsa.007350.1 vs. NCBI nr
Match: gi|659094501|ref|XP_008448094.1| (PREDICTED: mitogen-activated protein kinase kinase kinase 2 [Cucumis melo])

HSP 1 Score: 761.1 bits (1964), Expect = 9.7e-217
Identity = 372/401 (92.77%), Postives = 382/401 (95.26%), Query Frame = 1

Query: 1   MDWTRGPPVGRGSSATVYMATINSSGQQFAVKSTDFASSALLQKEHALLSNLSSPYLIKS 60
           MDWTRGPPVGRGSSATVYMAT+NSSGQQFAVKST+ ASSALL KEHALLSNLSSPYLIK 
Sbjct: 1   MDWTRGPPVGRGSSATVYMATLNSSGQQFAVKSTNLASSALLHKEHALLSNLSSPYLIKC 60

Query: 61  LGFDVETQPFNPPVFNLFMEYLPEGTLWDAIQSNGGRIEESMIRVFSRQILKGLDYLHAN 120
           LGFDVETQP NPPVFNLFMEY PEGTLWDAIQ NGGRIEESMI VFSRQILKGL+YLHAN
Sbjct: 61  LGFDVETQPLNPPVFNLFMEYFPEGTLWDAIQINGGRIEESMIGVFSRQILKGLEYLHAN 120

Query: 121 GLAHCDIKSRNLLLSDGDLKIADLGCAKFVDGISGNGVGAFSGSPAFMAPEVARGEEQGF 180
           GLAHCDIKSRNLLLSD  LKIADLGCAKFVDGISGNGVGAFSGSPAFMAPEVARGEEQGF
Sbjct: 121 GLAHCDIKSRNLLLSDEGLKIADLGCAKFVDGISGNGVGAFSGSPAFMAPEVARGEEQGF 180

Query: 181 PADVWAFGCTVIEMATGDHPWPEIEDPVSALYRIGFSGELPEIPRWLSEIARDFVAKCLI 240
           PADVWAFGCTVIEMATGDHPWPEIEDPVSALYRIGFSGE+PEIPRWLSE A+DFVAKCLI
Sbjct: 181 PADVWAFGCTVIEMATGDHPWPEIEDPVSALYRIGFSGEVPEIPRWLSESAKDFVAKCLI 240

Query: 241 KDSKERWSVKRLLEHPFLQELDSESEIEGSRMKMVNSSSPSCVLDQRFWDSMEDLETPWK 300
           KD KERWSVKRLLEHPFL+  DSESEIEGSRMK VNSSSPSCVLDQRFWDSMEDLE+P K
Sbjct: 241 KDPKERWSVKRLLEHPFLEGFDSESEIEGSRMKTVNSSSPSCVLDQRFWDSMEDLESPSK 300

Query: 301 VTQQASLVDCPAERIRRLADNFSCDLELEWDDGDWVLIRNNGVGGGSHGNGSVSIDFCAT 360
           VTQQASLVDCPAERIRRLADN SCDLELEWDDGDWVLIRNNGVGGGSHGNGSVS+DFCAT
Sbjct: 301 VTQQASLVDCPAERIRRLADNLSCDLELEWDDGDWVLIRNNGVGGGSHGNGSVSLDFCAT 360

Query: 361 VEEEDENLNSEIEENSVLFCLVDDDFSVVNSRKGGLQMELK 402
           +EEEDE LN EIEENSVLFCLVDDDFSVVNSR GGL+MELK
Sbjct: 361 IEEEDEILNLEIEENSVLFCLVDDDFSVVNSRNGGLEMELK 401

BLAST of Cucsa.007350.1 vs. NCBI nr
Match: gi|703100265|ref|XP_010096874.1| (Mitogen-activated protein kinase kinase kinase A [Morus notabilis])

HSP 1 Score: 372.5 bits (955), Expect = 9.7e-100
Identity = 194/375 (51.73%), Postives = 264/375 (70.40%), Query Frame = 1

Query: 1   MDWTRGPPVGRGSSATVYMATINSSGQQFAVKSTDFASSALLQKEHALLSNLSSPYLIKS 60
           M+WTRGP +GRG++ATV++AT   SG+ FAVKS +   +  L+KE + LS LSS  ++K 
Sbjct: 1   MEWTRGPTIGRGATATVHLATDTRSGEPFAVKSAELCRAVFLRKEQSFLSKLSSSRIVKY 60

Query: 61  LGFDVETQPFNPPVFNLFMEYLPEGTLWDAIQSNGGRIEESMIRVFSRQILKGLDYLHAN 120
           LGFDV T+  + P++NLF+EYLP GTL+DAI+S  GR++ES+I+++++QIL+GLD++H N
Sbjct: 61  LGFDVTTEN-DVPMYNLFIEYLPNGTLFDAIRSREGRLDESLIKIYTQQILQGLDHIHEN 120

Query: 121 GLAHCDIKSRNLLLSDGDLKIADLGCAKFV-DGISGNG--VGAFSGSPAFMAPEVARGEE 180
           GL HCDIKS+N+L+ +  +KIADLGCAKFV +   G+   V  FSG+PAFMAPEVARGE+
Sbjct: 121 GLVHCDIKSQNMLIGEEGIKIADLGCAKFVGEAEFGDSPLVLQFSGTPAFMAPEVARGEK 180

Query: 181 QGFPADVWAFGCTVIEMATGDHPWPEIEDPVSALYRIGFSGELPEIPRWLSEIARDFVAK 240
           QG  ADVWA GCTVIEMATG  PWPE++DPVSALYRIG+S  +PEIPRW SE  +DF++K
Sbjct: 181 QGLEADVWALGCTVIEMATGRGPWPEMDDPVSALYRIGYSDRVPEIPRWFSEKGKDFLSK 240

Query: 241 CLIKDSKERWSVKRLLEHPFLQELDSESEIEGSRMKMVNSSSPSCVLDQRFWDSMEDLET 300
           CL +D +ERW+V  LLEHPFLQ     SE+   +      SSPS VLD+  WDS E  E+
Sbjct: 241 CLKRDWRERWTVNELLEHPFLQ----VSELRSEQQVKAKMSSPSSVLDKDLWDSFEAFES 300

Query: 301 PWKVTQQASLVDCPAERIRRLADNFSCDLE--LEW--DDGDWVLIRNNGVGGGSHGN--- 360
                Q+AS ++   ERI+ L  +  C ++   +W  D+ +W  IR+N +    H     
Sbjct: 301 --HQDQEASSLNSAEERIKMLIRSAFCSVQNLPDWSCDEENWFTIRSNSIEEPIHEEELV 360

Query: 361 -GSVSIDFCATVEEE 365
            GS++ D   + +EE
Sbjct: 361 YGSITFDEELSFDEE 368

BLAST of Cucsa.007350.1 vs. NCBI nr
Match: gi|567920816|ref|XP_006452414.1| (hypothetical protein CICLE_v10010150mg [Citrus clementina])

HSP 1 Score: 370.5 bits (950), Expect = 3.7e-99
Identity = 188/356 (52.81%), Postives = 246/356 (69.10%), Query Frame = 1

Query: 1   MDWTRGPPVGRGSSATVYMATINSSGQQFAVKSTDFASSALLQKEHALLSNLSSPYLIKS 60
           M+WTRGP +GRGS+ATVY+A + SSG+  AVKST+ + S  LQ+E   LS +SSPY++  
Sbjct: 1   MEWTRGPIIGRGSTATVYLANLASSGKFIAVKSTELSQSMFLQREQCFLSKISSPYVVDY 60

Query: 61  LGFDVETQPFNPPVFNLFMEYLPEGTLWDAIQSNGGRIEESMIRVFSRQILKGLDYLHAN 120
           LGF+V T   N P +NL+MEY+P GTL+D I+ +GGR++E MI++++ QIL+GL++LH N
Sbjct: 61  LGFNV-TNENNTPTYNLYMEYMPRGTLYDEIRRHGGRLDEQMIKLYAWQILQGLNHLHMN 120

Query: 121 GLAHCDIKSRNLLLSDGDLKIADLGCAKFVDGISGNGV---GAFSGSPAFMAPEVARGEE 180
           G+AHCDIKS N+L+ D   KIADLGCAK+V  I  N      AFSG+P FMAPEVAR EE
Sbjct: 121 GIAHCDIKSNNVLIGDDGAKIADLGCAKYVGEIDSNAALATSAFSGTPVFMAPEVARREE 180

Query: 181 QGFPADVWAFGCTVIEMATGDHPWPEIEDPVSALYRIGFSGELPEIPRWLSEIARDFVAK 240
           QG  AD+WA GCT++EMATG +PWPE+ DPVSALYRIGFSGE PEIPRWLS  A+DF+ K
Sbjct: 181 QGLAADIWAVGCTIVEMATGTNPWPELNDPVSALYRIGFSGETPEIPRWLSMEAKDFLCK 240

Query: 241 CLIKDSKERWSVKRLLEHPFL--QELDSESEIEGSRMKMVNSSSPSCVLDQRFWDSMEDL 300
           CL KD KER + K LLEHPF+   E D   ++E   M     SSP  VL+  FWDS+E+ 
Sbjct: 241 CLTKDPKERLTAKELLEHPFICGVEFDHFKDVEKFIM-----SSPCTVLNHGFWDSLEEA 300

Query: 301 ETPWKVTQQAS-LVDCPAERIRRLADNFSCDLEL------EWD-DGDWVLIRNNGV 344
           E+   ++ +AS         ++R+     C L         W  D DW+ +R+NG+
Sbjct: 301 ESSENLSPKASPSTSSSNSAVKRIQQLIGCALPSLGSSVPNWSLDEDWITVRSNGI 350

BLAST of Cucsa.007350.1 vs. NCBI nr
Match: gi|661880558|emb|CDP15776.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 370.5 bits (950), Expect = 3.7e-99
Identity = 203/416 (48.80%), Postives = 275/416 (66.11%), Query Frame = 1

Query: 3   WTRGPPVGRGSSATVYMATINSSGQQFAVKSTDFASSALLQKEHALLSNLSSPYLIKSLG 62
           WTRGP +GRGSSATV +AT  +SG+  AVKS D + S++LQKE +L+S LSSPY++K +G
Sbjct: 4   WTRGPIIGRGSSATVSLAT-TASGELLAVKSADLSCSSVLQKEESLISELSSPYIVKYVG 63

Query: 63  FDVETQPFNPPVFNLFMEYLPEGTLWDAIQSNGGRIEESMIRVFSRQILKGLDYLHANGL 122
            DV T+  + P++NL MEY+P GTL D I+  GG +EESMI+VF+ QIL+GLDYLH NG+
Sbjct: 64  CDVTTEN-DKPLYNLLMEYVPGGTLSDQIRKQGGPLEESMIQVFAHQILQGLDYLHLNGI 123

Query: 123 AHCDIKSRNLLLSDGDLKIADLGCAKFVDGISGNGVGAFS---GSPAFMAPEVARGEEQG 182
            HCDIK +N+L+     KI DLGCA+ V+   GNG+   S   G+P FMAPEVARGEEQG
Sbjct: 124 VHCDIKGQNVLIGRDGAKIGDLGCARVVE--EGNGMAGKSVICGTPMFMAPEVARGEEQG 183

Query: 183 FPADVWAFGCTVIEMATGDHPWPEIEDPVSALYRIGFSGELPEIPRWLSEIARDFVAKCL 242
           F AD+WA GCT+IEMAT ++PWP++EDPVSALYRIG+SG++PE P WLS+ A++F++KCL
Sbjct: 184 FAADIWALGCTIIEMATANNPWPDMEDPVSALYRIGYSGDVPEFPCWLSDGAKEFLSKCL 243

Query: 243 IKDSKERWSVKRLLEHPFLQELDSESEIEGSRMKMVNSSSPSCVLDQRFWDSMEDLETPW 302
            +D+KERW+ + LL+HPF   +  E E E  R      +SP+ VLDQ FWDSME  E+  
Sbjct: 244 KRDAKERWTARELLQHPFFHGVGEEKEAEFVR------NSPTTVLDQGFWDSMEVAESSL 303

Query: 303 KVTQQ-ASLVDCPAERIRRLADN-FSCDLEL-EW-DDGDWVLIR-----------NNGVG 362
           + +    S +  PA+RI+ L  N FS +     W +DGDWV +R                
Sbjct: 304 QDSSHIVSSLGSPADRIKNLMGNLFSSNANFPNWSEDGDWVTVRGTDSQEIQPVSQQNEN 363

Query: 363 GGSHGNGSVSIDFCATVEEEDENLNSEIEENSVLFCLVDDDFSVVNSRKGGLQMEL 401
              H +      F  +++ E+E L S I  + +L   + D+ S V +  GGL + L
Sbjct: 364 SEVHDSQLAMDPFLYSIDLEEE-LESSIVIDDLLINCLVDEISNVGNVSGGLNLTL 408

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
NPK1_TOBAC1.9e-4237.17Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum GN=NPK1... [more]
M3K2_ARATH5.4e-4235.40Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana GN=ANP2... [more]
MKKA_DICDI9.2e-4234.42Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum GN=... [more]
ANP1_ARATH2.7e-4136.18Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana GN=A... [more]
M3K3_ARATH1.0e-4037.55Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana GN=ANP3... [more]
Match NameE-valueIdentityDescription
W9RB46_9ROSA6.7e-10051.73Mitogen-activated protein kinase kinase kinase A OS=Morus notabilis GN=L484_0080... [more]
V4WGB5_9ROSI2.6e-9952.81Uncharacterized protein OS=Citrus clementina GN=CICLE_v10010150mg PE=3 SV=1[more]
A0A068V557_COFCA2.6e-9948.80Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00015855001 PE=3 SV=1[more]
B9SVA5_RICCO7.4e-9954.52Mitogen-activated protein kinase kinase kinase, putative OS=Ricinus communis GN=... [more]
A0A067FF28_CITSI1.3e-9852.53Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g045508mg PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G26890.12.0e-9552.99 mitogen-activated protein kinase kinase kinase 16[more]
AT5G55090.13.8e-9451.86 mitogen-activated protein kinase kinase kinase 15[more]
AT2G32510.12.1e-8145.22 mitogen-activated protein kinase kinase kinase 17[more]
AT1G05100.14.2e-7746.31 mitogen-activated protein kinase kinase kinase 18[more]
AT4G36950.12.3e-5138.57 mitogen-activated protein kinase kinase kinase 21[more]
Match NameE-valueIdentityDescription
gi|778730258|ref|XP_011659740.1|2.3e-234100.00PREDICTED: mitogen-activated protein kinase kinase kinase 2, partial [Cucumis sa... [more]
gi|659094501|ref|XP_008448094.1|9.7e-21792.77PREDICTED: mitogen-activated protein kinase kinase kinase 2 [Cucumis melo][more]
gi|703100265|ref|XP_010096874.1|9.7e-10051.73Mitogen-activated protein kinase kinase kinase A [Morus notabilis][more]
gi|567920816|ref|XP_006452414.1|3.7e-9952.81hypothetical protein CICLE_v10010150mg [Citrus clementina][more]
gi|661880558|emb|CDP15776.1|3.7e-9948.80unnamed protein product [Coffea canephora][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR000719Prot_kinase_dom
IPR008271Ser/Thr_kinase_AS
IPR011009Kinase-like_dom_sf
IPR017441Protein_kinase_ATP_BS
Vocabulary: Molecular Function
TermDefinition
GO:0004672protein kinase activity
GO:0005524ATP binding
Vocabulary: Biological Process
TermDefinition
GO:0006468protein phosphorylation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005575 cellular_component
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsa.007350Cucsa.007350gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsa.007350.1Cucsa.007350.1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.007350.1.CDS.2Cucsa.007350.1.CDS.2CDS
Cucsa.007350.1.CDS.1Cucsa.007350.1.CDS.1CDS


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 7..258
score: 1.4
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 3..258
score: 2.0
IPR000719Protein kinase domainPROFILEPS50011PROTEIN_KINASE_DOMcoord: 3..258
score: 39
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 122..134
scor
IPR011009Protein kinase-like domainunknownSSF56112Protein kinase-like (PK-like)coord: 3..300
score: 1.75
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 9..32
scor
NoneNo IPR availableGENE3DG3DSA:1.10.510.10coord: 100..273
score: 3.8
NoneNo IPR availableGENE3DG3DSA:3.30.200.20coord: 6..99
score: 2.5
NoneNo IPR availablePANTHERPTHR24361MITOGEN-ACTIVATED KINASE KINASE KINASEcoord: 1..345
score: 1.1E
NoneNo IPR availablePANTHERPTHR24361:SF380MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 17-RELATEDcoord: 1..345
score: 1.1E