CsaV3_UNG052420.1 (mRNA) Cucumber (Chinese Long) v3

NameCsaV3_UNG052420.1
TypemRNA
OrganismCucumis sativus (Cucumber (Chinese Long) v3)
DescriptionIST1-like protein
Locationscaffold72 : 48006 .. 50530 (-)
Sequence length1614
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTAAGCAAGAGCTTCAAGCCTGCGAAATGGTAGGTTTGAGATTTCTTCAACTTAATCCTTGTAGTTTTGTTCTGTTGTTGGGTACTTTTGGCTGGGATCAATTTATTGACTTTTTTTTTTTTGCATTTTTTGTTTGAATTGGGTTGGGTCGGTGCAGTAAGACATCGCTGAAGTTAGCGGTTTCGCGGATTAAGCTTTTGAGGAATAAGAAAGATGTTCATATTAAGCAGCTTAAGGGAGATTTGGCTAAGTTGCTTGAGGCTGGACAAGACCAGACTGCTAGAATTCGGGTATTTTAGTGTTGTTCTTTGACTCTGGAATTGATTGTATTTATTTGTGTCTTTATCAAGTCACCGTGTTTTTTAGACCTAATGTCATATGGGAAGATGAACTTTATCGTGCGTCAATTTGATGATTCCTTTTCCGAAGAATTTACGTTTCCATCTTGTTTCAGGTTGAACACTTTGTTAGAGAAGAGAAATCGAAGGAAGCTTATGAACTCATTGAAATATTTTGTGAGCTAATTGTGGCGCGCATGCCAATGATTGAGTCCCAAAAGTATGTTAATTTCTTTTTTATTGAAGTTATTTTTGTTGATTTCTCATGAACTTCGCTGCCTATAGGGTATGCTTTGATTTCAGAATGTGCATTTGTATTCGACTTCACATGGTTGTGATTTCTTTTAAAGCAAAATTTTGCCCTCGTGACGTATGTGTTAACTTCTGATTTCTGAGCATAATGAATGTACTCCTATTAATTCGGTTTATGTTTTCATGTTCAGAAATTGTCCCATAGATTTGAAGGAAGCAGTCTCGAGTGTTATATTTGCATCACCCAGATGTGCAGATATTCCAGAGCTCTTGGATGTTCGTAAACACTTTAAATCAAAGTATGGAAAAGAATTTGTCTCGGCCGCAGTGGAGCTGCGCCCAGAATGTGGAGTGAATCGCATGGTAAATGTGTTAGCTGTTTTGTGTTCATAAACTTAGGGAGTGTGGGTTCTCTCTATGAAGATAACTTGGTTCCATTGACTTTCCTTCCATTCTTACAACTTTCCAGTTAGTCGAAAAACTATCTGCTAAAGCACCGGATGGGCAGACCAAACTTAAAATCTTGACTGCAATTGCTGAGGAGTATAACATCAAATGGGACCCAAAATCCTTTGGTGACAGCATCAACCCTCCGGCTGATTTGTTGGTAATTTTAGTTTTTATAAAAAAGAACTATTTCCTTCAATGCTCAAGGTGTTTTTATCAGGAAATGAAGACTTACCTGAATTATTTTATTTTAATATTGAATTTCGGTTTTAGTAATACTAGTTATGAACTTTGTTGAGCAGAGTGGACCAAATACTTTTGGGAAGGCTAGTCAAATACAGATGGAGTCTATTAGTGGCCCATCTTCATTTGATCACAAAGAATCTTCTAGAAAACATGTTCCATTCAAACCTGATGAAAGGCCTCATGTACCCGAGAGATCCCCTGAACACAGTTTGAGATCAGAACATCAGTCCAAACAATCCAACTTTGCTCACGTTAATGCCAATCAAAGCAACATCACTGGCCGTCATAATTCAGAAACATCTTTTGGTATGTGAAAATTATATCTGATGCAATCATCTTCATTTCTTTCTTCTCTATGTACGTGCTACACATACATATTGAAGACAGTTACTTTTGTTTGAATTTGCAATTGTTGGTTATATATTGAAACTGTGATATGCGTAAATTAATGCAGAGGGGATGCATAGGCACTCAAATTCTGGTGAACAAAATAATTACTCTTCAGGTAGGCAGCAGTGGAGTATGGATTTTAAGGATGCTACCTCTGCTGCAAAGGCAGCGGCTGAATCTGCAGAACTAGCAAGTCTAGCTGCCCGAGCTGCTGCAGAACTTTCCAGTCGTGGAAACATCTCCCAACCATCCTCTTCTGAGTTTCAGAAATCTTCGTCTTATAATTTAAGGGCAGAAGGACCTCAAGGATACCCTAGTGTCCATTTACGTGATCAACAGCTTCCTAAAGACCAAGTTGTCAGTGCACCTCGTAAAAGTTCTACGCCGGATGATAATTGGAGAGACAATGAGACAAGAACATACATGGGGGATAATTCCAAGAATTATAGTTATCCATCTTCAAGCGTTAGTAACAATGATGTCAACATATCTGTGACCAATCTCAGTGCAGCAGAAAGATCCTCTTTCAAGAAATCATCCGAACCTAGGTTTAGCGGTTCCCTTGGTAGTAGTGCAACTTTGGAGAAACAAACCAGAAAACATGATGCCAGCACGTCTGTAACCAGTTTCAATGCTGCAGATAGATACTCTTTCAAGAATTCATTCGAACCTGGGGTCAGTGGCTCTCTTGATAGTGGGGATGAACAACCCAGAAATTTTAGTTCCAACATATCTTCCACTAATTTCAACGAATCAGACACATACTCGCTCAACAAGCCGTCTGAATCTGGGTTTAGTGACTCTTTAGGTAGAACCAGTATGGAGAAACAACCCGAAATGTTGATGTAG

mRNA sequence

ATGCTAAGCAAGAGCTTCAAGCCTGCGAAATGTAAGACATCGCTGAAGTTAGCGGTTTCGCGGATTAAGCTTTTGAGGAATAAGAAAGATGTTCATATTAAGCAGCTTAAGGGAGATTTGGCTAAGTTGCTTGAGGCTGGACAAGACCAGACTGCTAGAATTCGGGTTGAACACTTTGTTAGAGAAGAGAAATCGAAGGAAGCTTATGAACTCATTGAAATATTTTGTGAGCTAATTGTGGCGCGCATGCCAATGATTGAGTCCCAAAAAAATTGTCCCATAGATTTGAAGGAAGCAGTCTCGAGTGTTATATTTGCATCACCCAGATGTGCAGATATTCCAGAGCTCTTGGATGTTCGTAAACACTTTAAATCAAAGTATGGAAAAGAATTTGTCTCGGCCGCAGTGGAGCTGCGCCCAGAATGTGGAGTGAATCGCATGTTAGTCGAAAAACTATCTGCTAAAGCACCGGATGGGCAGACCAAACTTAAAATCTTGACTGCAATTGCTGAGGAGTATAACATCAAATGGGACCCAAAATCCTTTGGTGACAGCATCAACCCTCCGGCTGATTTGTTGAGTGGACCAAATACTTTTGGGAAGGCTAGTCAAATACAGATGGAGTCTATTAGTGGCCCATCTTCATTTGATCACAAAGAATCTTCTAGAAAACATGTTCCATTCAAACCTGATGAAAGGCCTCATGTACCCGAGAGATCCCCTGAACACAGTTTGAGATCAGAACATCAGTCCAAACAATCCAACTTTGCTCACGTTAATGCCAATCAAAGCAACATCACTGGCCGTCATAATTCAGAAACATCTTTTGAGGGGATGCATAGGCACTCAAATTCTGGTGAACAAAATAATTACTCTTCAGGTAGGCAGCAGTGGAGTATGGATTTTAAGGATGCTACCTCTGCTGCAAAGGCAGCGGCTGAATCTGCAGAACTAGCAAGTCTAGCTGCCCGAGCTGCTGCAGAACTTTCCAGTCGTGGAAACATCTCCCAACCATCCTCTTCTGAGTTTCAGAAATCTTCGTCTTATAATTTAAGGGCAGAAGGACCTCAAGGATACCCTAGTGTCCATTTACGTGATCAACAGCTTCCTAAAGACCAAGTTGTCAGTGCACCTCGTAAAAGTTCTACGCCGGATGATAATTGGAGAGACAATGAGACAAGAACATACATGGGGGATAATTCCAAGAATTATAGTTATCCATCTTCAAGCGTTAGTAACAATGATGTCAACATATCTGTGACCAATCTCAGTGCAGCAGAAAGATCCTCTTTCAAGAAATCATCCGAACCTAGGTTTAGCGGTTCCCTTGGTAGTAGTGCAACTTTGGAGAAACAAACCAGAAAACATGATGCCAGCACGTCTGTAACCAGTTTCAATGCTGCAGATAGATACTCTTTCAAGAATTCATTCGAACCTGGGGTCAGTGGCTCTCTTGATAGTGGGGATGAACAACCCAGAAATTTTAGTTCCAACATATCTTCCACTAATTTCAACGAATCAGACACATACTCGCTCAACAAGCCGTCTGAATCTGGGTTTAGTGACTCTTTAGGTAGAACCAGTATGGAGAAACAACCCGAAATGTTGATGTAG

Coding sequence (CDS)

ATGCTAAGCAAGAGCTTCAAGCCTGCGAAATGTAAGACATCGCTGAAGTTAGCGGTTTCGCGGATTAAGCTTTTGAGGAATAAGAAAGATGTTCATATTAAGCAGCTTAAGGGAGATTTGGCTAAGTTGCTTGAGGCTGGACAAGACCAGACTGCTAGAATTCGGGTTGAACACTTTGTTAGAGAAGAGAAATCGAAGGAAGCTTATGAACTCATTGAAATATTTTGTGAGCTAATTGTGGCGCGCATGCCAATGATTGAGTCCCAAAAAAATTGTCCCATAGATTTGAAGGAAGCAGTCTCGAGTGTTATATTTGCATCACCCAGATGTGCAGATATTCCAGAGCTCTTGGATGTTCGTAAACACTTTAAATCAAAGTATGGAAAAGAATTTGTCTCGGCCGCAGTGGAGCTGCGCCCAGAATGTGGAGTGAATCGCATGTTAGTCGAAAAACTATCTGCTAAAGCACCGGATGGGCAGACCAAACTTAAAATCTTGACTGCAATTGCTGAGGAGTATAACATCAAATGGGACCCAAAATCCTTTGGTGACAGCATCAACCCTCCGGCTGATTTGTTGAGTGGACCAAATACTTTTGGGAAGGCTAGTCAAATACAGATGGAGTCTATTAGTGGCCCATCTTCATTTGATCACAAAGAATCTTCTAGAAAACATGTTCCATTCAAACCTGATGAAAGGCCTCATGTACCCGAGAGATCCCCTGAACACAGTTTGAGATCAGAACATCAGTCCAAACAATCCAACTTTGCTCACGTTAATGCCAATCAAAGCAACATCACTGGCCGTCATAATTCAGAAACATCTTTTGAGGGGATGCATAGGCACTCAAATTCTGGTGAACAAAATAATTACTCTTCAGGTAGGCAGCAGTGGAGTATGGATTTTAAGGATGCTACCTCTGCTGCAAAGGCAGCGGCTGAATCTGCAGAACTAGCAAGTCTAGCTGCCCGAGCTGCTGCAGAACTTTCCAGTCGTGGAAACATCTCCCAACCATCCTCTTCTGAGTTTCAGAAATCTTCGTCTTATAATTTAAGGGCAGAAGGACCTCAAGGATACCCTAGTGTCCATTTACGTGATCAACAGCTTCCTAAAGACCAAGTTGTCAGTGCACCTCGTAAAAGTTCTACGCCGGATGATAATTGGAGAGACAATGAGACAAGAACATACATGGGGGATAATTCCAAGAATTATAGTTATCCATCTTCAAGCGTTAGTAACAATGATGTCAACATATCTGTGACCAATCTCAGTGCAGCAGAAAGATCCTCTTTCAAGAAATCATCCGAACCTAGGTTTAGCGGTTCCCTTGGTAGTAGTGCAACTTTGGAGAAACAAACCAGAAAACATGATGCCAGCACGTCTGTAACCAGTTTCAATGCTGCAGATAGATACTCTTTCAAGAATTCATTCGAACCTGGGGTCAGTGGCTCTCTTGATAGTGGGGATGAACAACCCAGAAATTTTAGTTCCAACATATCTTCCACTAATTTCAACGAATCAGACACATACTCGCTCAACAAGCCGTCTGAATCTGGGTTTAGTGACTCTTTAGGTAGAACCAGTATGGAGAAACAACCCGAAATGTTGATGTAG

Protein sequence

MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFVREEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVRKHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPKSFGDSINPPADLLSGPNTFGKASQIQMESISGPSSFDHKESSRKHVPFKPDERPHVPERSPEHSLRSEHQSKQSNFAHVNANQSNITGRHNSETSFEGMHRHSNSGEQNNYSSGRQQWSMDFKDATSAAKAAAESAELASLAARAAAELSSRGNISQPSSSEFQKSSSYNLRAEGPQGYPSVHLRDQQLPKDQVVSAPRKSSTPDDNWRDNETRTYMGDNSKNYSYPSSSVSNNDVNISVTNLSAAERSSFKKSSEPRFSGSLGSSATLEKQTRKHDASTSVTSFNAADRYSFKNSFEPGVSGSLDSGDEQPRNFSSNISSTNFNESDTYSLNKPSESGFSDSLGRTSMEKQPEMLM
BLAST of CsaV3_UNG052420.1 vs. NCBI nr
Match: XP_004135813.1 (PREDICTED: uncharacterized protein LOC101217093 [Cucumis sativus] >KGN66208.1 hypothetical protein Csa_1G580230 [Cucumis sativus])

HSP 1 Score: 635.2 bits (1637), Expect = 2.0e-178
Identity = 453/453 (100.00%), Postives = 453/453 (100.00%), Query Frame = 0

Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV
Sbjct: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120
           REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR
Sbjct: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120

Query: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180
           KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK
Sbjct: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180

Query: 181 SFGDSINPPADLLSGXXXXXXXXXXXXXXXXXXXXXDHKESSRKHVPXXXXXXXXXXXXX 240
           SFGDSINPPADLLSGXXXXXXXXXXXXXXXXXXXXXDHKESSRKHVPXXXXXXXXXXXXX
Sbjct: 181 SFGDSINPPADLLSGXXXXXXXXXXXXXXXXXXXXXDHKESSRKHVPXXXXXXXXXXXXX 240

Query: 241 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQNNYSSGRQQWSM 300
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQNNYSSGRQQWSM
Sbjct: 241 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQNNYSSGRQQWSM 300

Query: 301 DFKDATXXXXXXXXXXXXXXXXXXXXXXXXSRGNISQPSSSEFQKSSSYNLRAEGPQGYP 360
           DFKDATXXXXXXXXXXXXXXXXXXXXXXXXSRGNISQPSSSEFQKSSSYNLRAEGPQGYP
Sbjct: 301 DFKDATXXXXXXXXXXXXXXXXXXXXXXXXSRGNISQPSSSEFQKSSSYNLRAEGPQGYP 360

Query: 361 SVHLRDQQLPKDQVVSAPRKSSTPDDNWRDNETRTYMGDNSKNYSYPSSSVSNNDVNISV 420
           SVHLRDQQLPKDQVVSAPRKSSTPDDNWRDNETRTYMGDNSKNYSYPSSSVSNNDVNISV
Sbjct: 361 SVHLRDQQLPKDQVVSAPRKSSTPDDNWRDNETRTYMGDNSKNYSYPSSSVSNNDVNISV 420

Query: 421 TNLSAAERSSFKKSSEPRFSGSLGSSATLEKQT 454
           TNLSAAERSSFKKSSEPRFSGSLGSSATLEKQT
Sbjct: 421 TNLSAAERSSFKKSSEPRFSGSLGSSATLEKQT 453

BLAST of CsaV3_UNG052420.1 vs. NCBI nr
Match: XP_008450820.1 (PREDICTED: uncharacterized protein LOC103492291 [Cucumis melo])

HSP 1 Score: 607.8 bits (1566), Expect = 3.5e-170
Identity = 416/452 (92.04%), Postives = 422/452 (93.36%), Query Frame = 0

Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           ML+KSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV
Sbjct: 1   MLNKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120
           REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR
Sbjct: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120

Query: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180
           KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQ+KLKILTAIAEEYNIKWDPK
Sbjct: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQSKLKILTAIAEEYNIKWDPK 180

Query: 181 SFGDSINPPADLLSGXXXXXXXXXXXXXXXXXXXXXDHKESSRKHVPXXXXXXXXXXXXX 240
           SFGDSINPPADLL+G                     D KESSRKHVP XXXXXXXXXXXX
Sbjct: 181 SFGDSINPPADLLNGPNTFGKASQIQMESIGGPSSFDRKESSRKHVPFXXXXXXXXXXXX 240

Query: 241 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQNNYSSGRQQWSM 300
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQNNYSSGRQQWSM
Sbjct: 241 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQNNYSSGRQQWSM 300

Query: 301 DFKDATXXXXXXXXXXXXXXXXXXXXXXXXSRGNISQPSSSEFQKSSSYNLRAEGPQGYP 360
           DFKDATXXXXXXXXXXXXXXXXXXXXXXXXSRGNISQPSSSEFQKSSSYN+RAEGPQGYP
Sbjct: 301 DFKDATXXXXXXXXXXXXXXXXXXXXXXXXSRGNISQPSSSEFQKSSSYNVRAEGPQGYP 360

Query: 361 SVHLRDQQLPKDQVVSAPRKSSTPDDNWRDNETRTYMGDNSKNYSYPSSSVSNNDVNISV 420
           SVHL DQQLPKDQVV APRKSSTPDDNWRDNETR YMGDNSKN SYPSSSVSNNDVN SV
Sbjct: 361 SVHLHDQQLPKDQVVGAPRKSSTPDDNWRDNETRRYMGDNSKNSSYPSSSVSNNDVNTSV 420

Query: 421 TNLSAAERSSFKKSSEPRFSGSLGSSATLEKQ 453
           TN +AAERSSFK SSEPRFSGSLGSSAT+EKQ
Sbjct: 421 TNFNAAERSSFKNSSEPRFSGSLGSSATVEKQ 452

BLAST of CsaV3_UNG052420.1 vs. NCBI nr
Match: XP_022927973.1 (filaggrin isoform X1 [Cucurbita moschata])

HSP 1 Score: 537.3 bits (1383), Expect = 5.8e-149
Identity = 322/464 (69.40%), Postives = 348/464 (75.00%), Query Frame = 0

Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           ML KSFKPAKCKTSLKLAVSRIKLLRNKKDVH+KQLKG+LAKLLEAGQDQTARIRVEHFV
Sbjct: 1   MLHKSFKPAKCKTSLKLAVSRIKLLRNKKDVHVKQLKGELAKLLEAGQDQTARIRVEHFV 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120
           REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKE+VSSVIFASPRCADIPELLDVR
Sbjct: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKESVSSVIFASPRCADIPELLDVR 120

Query: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180
           KHFK+KYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDG +K+KILT IAEEYN+KWDPK
Sbjct: 121 KHFKAKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGPSKIKILTKIAEEYNVKWDPK 180

Query: 181 SFGDSINPPADLLSGXXXXXXXXXXXXXXXXXXXXXDH------------KESSRKHVPX 240
           SFGD+INPPADLL+G                     DH            +   R+ +P 
Sbjct: 181 SFGDNINPPADLLNGPNTFGRASQIQMEAIGGQPSLDHNNRGSPNIQAPPESDERQRIP- 240

Query: 241 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQ 300
                                                                      Q
Sbjct: 241 ----EDPVNRNLRSNHHPQQPNFADVNANQSNFTGHRNSEARSSETSAEGMRRHSNSGDQ 300

Query: 301 NNYSSGRQQWSMDFKDATXXXXXXXXXXXXXXXXXXXXXXXXSRGNISQPSSSEFQKSSS 360
           N+Y+SGRQ W MDFKDATXXXXXXXXXXXXXXXXXXXXXXXXSRGNISQPSSSEF +SSS
Sbjct: 301 NSYASGRQHWGMDFKDATXXXXXXXXXXXXXXXXXXXXXXXXSRGNISQPSSSEFHQSSS 360

Query: 361 YNLRAEGPQGYPSVHLRDQQLPKDQVVSAPRKSSTPDDNWRDNETRTYMGDNSKNYSYPS 420
           YNLRAEGPQGY S +LRDQQLPKDQVVSAP KSS PDDNWRDN+TR +MG+++KN+SYPS
Sbjct: 361 YNLRAEGPQGYASGNLRDQQLPKDQVVSAPHKSSMPDDNWRDNDTRRFMGNDAKNFSYPS 420

Query: 421 SSVSNNDVNISVTNLSAAERSSFKKSSEPRFSGSLGSSATLEKQ 453
           SS SNNDVNIS TN +AA+R SFK SSEP F  SLGSSA++EKQ
Sbjct: 421 SSASNNDVNISATNFNAADRYSFKNSSEPGFRDSLGSSASVEKQ 459

BLAST of CsaV3_UNG052420.1 vs. NCBI nr
Match: XP_023002515.1 (uncharacterized protein LOC111496330 isoform X1 [Cucurbita maxima])

HSP 1 Score: 537.3 bits (1383), Expect = 5.8e-149
Identity = 323/464 (69.61%), Postives = 348/464 (75.00%), Query Frame = 0

Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           ML KSFKPAKCKTSLKLAVSRIKLLRNKKDVH+KQLKG+LAKLLEAGQDQTARIRVEHFV
Sbjct: 1   MLHKSFKPAKCKTSLKLAVSRIKLLRNKKDVHVKQLKGELAKLLEAGQDQTARIRVEHFV 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120
           REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKE+VSSVIFASPRCADIPELLDVR
Sbjct: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKESVSSVIFASPRCADIPELLDVR 120

Query: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180
           KHFK+KYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDG +K+KILT IAEEYN+KWDPK
Sbjct: 121 KHFKAKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGPSKIKILTKIAEEYNVKWDPK 180

Query: 181 SFGDSINPPADLLSGXXXXXXXXXXXXXXXXXXXXXDH------------KESSRKHVPX 240
           SFGD+INPPADLL+G                     DH            +   R+ +P 
Sbjct: 181 SFGDNINPPADLLNGPNTFGRASQIQMEAIGGQPSFDHNNRGSPNIQAPPESDERQRIP- 240

Query: 241 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQ 300
                                                                      Q
Sbjct: 241 ----EDPVNRNLRSNHHTQQSNFADVNANQSNFTGHRNSEARSSETSAEGMHRHSNSGDQ 300

Query: 301 NNYSSGRQQWSMDFKDATXXXXXXXXXXXXXXXXXXXXXXXXSRGNISQPSSSEFQKSSS 360
           NNY+SGRQ WSMDFKDATXXXXXXXXXXXXXXXXXXXXXXXXSRGN+SQPSSSEF KSSS
Sbjct: 301 NNYASGRQHWSMDFKDATXXXXXXXXXXXXXXXXXXXXXXXXSRGNLSQPSSSEFLKSSS 360

Query: 361 YNLRAEGPQGYPSVHLRDQQLPKDQVVSAPRKSSTPDDNWRDNETRTYMGDNSKNYSYPS 420
           YNLRAEGPQGY S +LRDQQLPKDQVVSAP  SS PDDNWRDN+TR +MG+++KN+SYPS
Sbjct: 361 YNLRAEGPQGYASGNLRDQQLPKDQVVSAPHNSSMPDDNWRDNDTRRFMGNDAKNFSYPS 420

Query: 421 SSVSNNDVNISVTNLSAAERSSFKKSSEPRFSGSLGSSATLEKQ 453
           SS SNNDVNIS TN +AA+R SFK SSE  FS SLGSSA++EKQ
Sbjct: 421 SSASNNDVNISATNFNAADRYSFKNSSEHGFSDSLGSSASVEKQ 459

BLAST of CsaV3_UNG052420.1 vs. NCBI nr
Match: XP_023531863.1 (filaggrin-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 534.6 bits (1376), Expect = 3.8e-148
Identity = 320/464 (68.97%), Postives = 346/464 (74.57%), Query Frame = 0

Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           ML KSFKPAKCKTSLKLAVSRIKLLRNKKDVH+KQLKG+LAKLLEAGQDQTARIRVEHFV
Sbjct: 1   MLHKSFKPAKCKTSLKLAVSRIKLLRNKKDVHVKQLKGELAKLLEAGQDQTARIRVEHFV 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120
           REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKE+VSSVIFASPRCADIPELLDVR
Sbjct: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKESVSSVIFASPRCADIPELLDVR 120

Query: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180
           KHFK+KYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDG +K+KILT IAEEYN+KWDPK
Sbjct: 121 KHFKAKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGPSKIKILTKIAEEYNVKWDPK 180

Query: 181 SFGDSINPPADLLSGXXXXXXXXXXXXXXXXXXXXXDH------------KESSRKHVPX 240
           SFGD+INPPADLL+G                     DH            +   R+ +P 
Sbjct: 181 SFGDNINPPADLLNGPNTFGRASQIQMEAIGGQPSLDHNNRGSPNIQAPPESDERQRIP- 240

Query: 241 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQ 300
                                                                      Q
Sbjct: 241 ----EDPVNRNLRSNHHPQQSNFADVNANQSNFTGHRNSEARSSETSAEGMHRYSNSGDQ 300

Query: 301 NNYSSGRQQWSMDFKDATXXXXXXXXXXXXXXXXXXXXXXXXSRGNISQPSSSEFQKSSS 360
           NNY+SGRQ W MDFKDATXXXXXXXXXXXXXXXXXXXXXXXXSRGN+SQPSSSEF +SSS
Sbjct: 301 NNYASGRQHWGMDFKDATXXXXXXXXXXXXXXXXXXXXXXXXSRGNVSQPSSSEFHQSSS 360

Query: 361 YNLRAEGPQGYPSVHLRDQQLPKDQVVSAPRKSSTPDDNWRDNETRTYMGDNSKNYSYPS 420
           YNLRAEGPQGY S +LRDQQLPKDQ VSAP KSS PDDNWRDN+TR +MG+++KN+SYPS
Sbjct: 361 YNLRAEGPQGYASGNLRDQQLPKDQFVSAPHKSSMPDDNWRDNDTRRFMGNDAKNFSYPS 420

Query: 421 SSVSNNDVNISVTNLSAAERSSFKKSSEPRFSGSLGSSATLEKQ 453
           SS SNNDVNIS TN +AA+R S K SSEP F  SLGSSA++EKQ
Sbjct: 421 SSASNNDVNISATNFNAADRYSLKNSSEPGFRDSLGSSASVEKQ 459

BLAST of CsaV3_UNG052420.1 vs. TAIR10
Match: AT2G19710.1 (Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 250.8 bits (639), Expect = 2.0e-66
Identity = 124/195 (63.59%), Postives = 164/195 (84.10%), Query Frame = 0

Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           +L + FKPAKCKT+L++A SR+K+L+NKK++ IKQL+ +LA+LLE+GQ  TARIRVEH V
Sbjct: 4   VLQRGFKPAKCKTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARIRVEHVV 63

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120
           REEK+  AYELI I+CEL+V R+ +IESQKNCPIDLKEAV+SV+FAS R +D+PEL ++ 
Sbjct: 64  REEKTVAAYELIGIYCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVPELSEIF 123

Query: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180
           K F +KYGK+F ++AVELRP+ GV+R+LVEKLSAKAPDG TK+KIL AIAEE+N+ W+ +
Sbjct: 124 KQFTTKYGKDFSTSAVELRPDSGVSRLLVEKLSAKAPDGPTKVKILMAIAEEHNVVWEAQ 183

Query: 181 SFGDSINPPADLLSG 196
           SF +S     +LL+G
Sbjct: 184 SFVESDPKDTELLNG 198

BLAST of CsaV3_UNG052420.1 vs. TAIR10
Match: AT4G29440.1 (Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 236.1 bits (601), Expect = 5.0e-62
Identity = 115/185 (62.16%), Postives = 153/185 (82.70%), Query Frame = 0

Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           +L +SFKPAKCK +L++A SR+K+L+NKKD  IKQL+ +LA LLE+GQ QTA+IRVEH V
Sbjct: 4   VLHRSFKPAKCKIALQMAASRLKILKNKKDTQIKQLRRELAHLLESGQTQTAKIRVEHVV 63

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120
           REEK+  AYEL+ I+CEL+VAR+ +I+SQK CP DLKEAV+SV++AS R  D+ EL D+ 
Sbjct: 64  REEKTVAAYELVGIYCELLVARLGVIDSQKTCPNDLKEAVASVLYASQRLTDVGELSDIV 123

Query: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180
           KHF +KYGK+FVSAA+ L+P+ GV+R+LVEKLS KAPDG TK+KILT IA ++N+ W+ +
Sbjct: 124 KHFSAKYGKDFVSAAIGLQPDSGVSRLLVEKLSVKAPDGPTKIKILTEIATQHNVTWEAE 183

Query: 181 SFGDS 186
           S  +S
Sbjct: 184 SLVES 188

BLAST of CsaV3_UNG052420.1 vs. TAIR10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 218.8 bits (556), Expect = 8.3e-57
Identity = 102/178 (57.30%), Postives = 142/178 (79.78%), Query Frame = 0

Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           +  + F  +KCKT+ K+AV+RIKL+RNK+ V +KQ++ D+A LL++GQD TARIRVEH +
Sbjct: 21  LFRRGFNSSKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHVI 80

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120
           RE+  + A E+IE+FCELIV+R+ +I  QK CP+DLKE ++S+IFA+PRC++IPEL D+R
Sbjct: 81  REQNIQAANEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDLR 140

Query: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWD 179
             F  KYGK+FVSAA +LRP CGVNRML++KLS + P G+ KLKI+  IA+E+ + WD
Sbjct: 141 DIFAKKYGKDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKEFQVDWD 198

BLAST of CsaV3_UNG052420.1 vs. TAIR10
Match: AT1G34220.1 (Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 213.0 bits (541), Expect = 4.5e-55
Identity = 114/224 (50.89%), Postives = 147/224 (65.62%), Query Frame = 0

Query: 3   SKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFVRE 62
           +K FK AKCKT LKL + RIKL+RN+++  IKQ++ ++AKLLE GQ+ TARIRVEH +RE
Sbjct: 9   NKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVEHIIRE 68

Query: 63  EKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVRKH 122
           EK   A E++E+FCELI  R+P+IE+Q+ CP+DLKEA+SSV FA+PRC+D+ EL  V+  
Sbjct: 69  EKMMAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQQVQIL 128

Query: 123 FKSKYGKEFVSAAVELRPECGVNR------------------------------MLVEKL 182
           F SKYGKEFV+AA EL+P+ GVNR                               LVE L
Sbjct: 129 FVSKYGKEFVAAASELKPDSGVNRKTESLIFIAWFSLVETRDLFMFLYFSNSILQLVELL 188

Query: 183 SAKAPDGQTKLKILTAIAEEYNIKWDPKSF-GDSINPPADLLSG 196
           S +AP  +TKLK+L  IAEE+ + WDP S   D      DLL G
Sbjct: 189 SVRAPSPETKLKLLKEIAEEHELDWDPASTETDLFKSHEDLLDG 232

BLAST of CsaV3_UNG052420.1 vs. TAIR10
Match: AT1G25420.1 (Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 208.4 bits (529), Expect = 1.1e-53
Identity = 96/188 (51.06%), Postives = 143/188 (76.06%), Query Frame = 0

Query: 9   AKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFVREEKSKEA 68
           AKCKTSL LA++R+KLL+NK+D+ +K +K ++A  L+AGQ+  ARIRVEH +RE     A
Sbjct: 15  AKCKTSLNLAIARMKLLQNKRDMQLKHMKKEIAHFLQAGQEPIARIRVEHVIREMNLWAA 74

Query: 69  YELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVRKHFKSKYG 128
           YE++E+FCE I+AR+P++ES+K CP +L+EA++S+IFA+PRC+++P+LL ++  F +KYG
Sbjct: 75  YEILELFCEFILARVPILESEKECPRELREAIASIIFAAPRCSEVPDLLQIKNLFGTKYG 134

Query: 129 KEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPKSF-GDSIN 188
           KEF+  A ELRP+ GVNR ++EKLS  +P G  +LK+L  IA+EY++ WD  +   + + 
Sbjct: 135 KEFIMVASELRPDSGVNRTIIEKLSPTSPSGAARLKMLKEIAQEYSLNWDSSATEAEFMK 194

Query: 189 PPADLLSG 196
              DLL G
Sbjct: 195 SHEDLLGG 202

BLAST of CsaV3_UNG052420.1 vs. Swiss-Prot
Match: sp|Q54I39|IST1L_DICDI (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 5.5e-26
Identity = 69/189 (36.51%), Postives = 112/189 (59.26%), Query Frame = 0

Query: 5   SFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFVREEK 64
           S+   K K  LKLAVSRI++L+NKK   ++  K ++A+LL    +++ARIRVE  +R+E 
Sbjct: 7   SYDSYKLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRDEY 66

Query: 65  SKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVRKHFK 124
             E +++IE+ CEL+ AR+ +I +    P+++KE++ +++++S R   IPEL  ++   K
Sbjct: 67  LIECFQIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQR-IQIPELEQIKNQLK 126

Query: 125 SKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPKSFGD 184
           +KYGK   + A        VN  +V KLS   PD     + L+ IAE++N+ W     G 
Sbjct: 127 AKYGKGLENEA-NCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEKFNVDW----CGS 186

Query: 185 SINPPADLL 194
              PP  L+
Sbjct: 187 DYPPPPQLI 189

BLAST of CsaV3_UNG052420.1 vs. Swiss-Prot
Match: sp|P53990|IST1_HUMAN (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 6.1e-25
Identity = 69/190 (36.32%), Postives = 112/190 (58.95%), Query Frame = 0

Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           ML   FK  + + +L+L ++R+KLL  KK    ++ + ++A  L AG+D+ ARIRVEH +
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRC-ADIPELLDV 120
           RE+   EA E++E++C+L++AR  +I+S K     L E+VS++I+A+PR  +++ EL  V
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 121 RKHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDP 180
                +KY KE+            VN  L+ KLS +AP      + L  IA+ YN+ ++P
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 180

Query: 181 KSFGDSINPP 190
            S   +  PP
Sbjct: 181 DSVVMAEAPP 189

BLAST of CsaV3_UNG052420.1 vs. Swiss-Prot
Match: sp|Q9CX00|IST1_MOUSE (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 1.4e-24
Identity = 67/182 (36.81%), Postives = 109/182 (59.89%), Query Frame = 0

Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           ML   FK  + + +L+L ++R+KLL  KK    ++ + ++A  L AG+D+ ARIRVEH +
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRC-ADIPELLDV 120
           RE+   EA E++E++C+L++AR  +I+S K     L E+VS++I+A+PR  +++ EL  V
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 121 RKHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDP 180
                +KY KE+            VN  L+ KLS +AP      + L  IA+ YN+ ++P
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 180

Query: 181 KS 182
            S
Sbjct: 181 DS 181

BLAST of CsaV3_UNG052420.1 vs. Swiss-Prot
Match: sp|Q568Z6|IST1_RAT (IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 1.4e-24
Identity = 67/182 (36.81%), Postives = 109/182 (59.89%), Query Frame = 0

Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           ML   FK  + + +L+L ++R+KLL  KK    ++ + ++A  L AG+D+ ARIRVEH +
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRC-ADIPELLDV 120
           RE+   EA E++E++C+L++AR  +I+S K     L E+VS++I+A+PR  +++ EL  V
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 121 RKHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDP 180
                +KY KE+            VN  L+ KLS +AP      + L  IA+ YN+ ++P
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 180

Query: 181 KS 182
            S
Sbjct: 181 DS 181

BLAST of CsaV3_UNG052420.1 vs. Swiss-Prot
Match: sp|Q3ZBV1|IST1_BOVIN (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 3.0e-24
Identity = 68/190 (35.79%), Postives = 111/190 (58.42%), Query Frame = 0

Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           ML    K  + + +L+L ++R+KLL  KK    ++ + ++A  L AG+D+ ARIRVEH +
Sbjct: 1   MLGSGIKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRC-ADIPELLDV 120
           RE+   EA E++E++C+L++AR  +I+S K     L E+VS++I+A+PR  +++ EL  V
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 121 RKHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDP 180
                +KY KE+            VN  L+ KLS +AP      + L  IA+ YN+ ++P
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEP 180

Query: 181 KSFGDSINPP 190
            S   +  PP
Sbjct: 181 DSVVMAEAPP 189

BLAST of CsaV3_UNG052420.1 vs. TrEMBL
Match: tr|A0A0A0LWR1|A0A0A0LWR1_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G580230 PE=4 SV=1)

HSP 1 Score: 635.2 bits (1637), Expect = 1.3e-178
Identity = 453/453 (100.00%), Postives = 453/453 (100.00%), Query Frame = 0

Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV
Sbjct: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120
           REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR
Sbjct: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120

Query: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180
           KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK
Sbjct: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180

Query: 181 SFGDSINPPADLLSGXXXXXXXXXXXXXXXXXXXXXDHKESSRKHVPXXXXXXXXXXXXX 240
           SFGDSINPPADLLSGXXXXXXXXXXXXXXXXXXXXXDHKESSRKHVPXXXXXXXXXXXXX
Sbjct: 181 SFGDSINPPADLLSGXXXXXXXXXXXXXXXXXXXXXDHKESSRKHVPXXXXXXXXXXXXX 240

Query: 241 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQNNYSSGRQQWSM 300
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQNNYSSGRQQWSM
Sbjct: 241 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQNNYSSGRQQWSM 300

Query: 301 DFKDATXXXXXXXXXXXXXXXXXXXXXXXXSRGNISQPSSSEFQKSSSYNLRAEGPQGYP 360
           DFKDATXXXXXXXXXXXXXXXXXXXXXXXXSRGNISQPSSSEFQKSSSYNLRAEGPQGYP
Sbjct: 301 DFKDATXXXXXXXXXXXXXXXXXXXXXXXXSRGNISQPSSSEFQKSSSYNLRAEGPQGYP 360

Query: 361 SVHLRDQQLPKDQVVSAPRKSSTPDDNWRDNETRTYMGDNSKNYSYPSSSVSNNDVNISV 420
           SVHLRDQQLPKDQVVSAPRKSSTPDDNWRDNETRTYMGDNSKNYSYPSSSVSNNDVNISV
Sbjct: 361 SVHLRDQQLPKDQVVSAPRKSSTPDDNWRDNETRTYMGDNSKNYSYPSSSVSNNDVNISV 420

Query: 421 TNLSAAERSSFKKSSEPRFSGSLGSSATLEKQT 454
           TNLSAAERSSFKKSSEPRFSGSLGSSATLEKQT
Sbjct: 421 TNLSAAERSSFKKSSEPRFSGSLGSSATLEKQT 453

BLAST of CsaV3_UNG052420.1 vs. TrEMBL
Match: tr|A0A1S3BQ24|A0A1S3BQ24_CUCME (uncharacterized protein LOC103492291 OS=Cucumis melo OX=3656 GN=LOC103492291 PE=4 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 2.3e-170
Identity = 416/452 (92.04%), Postives = 422/452 (93.36%), Query Frame = 0

Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           ML+KSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV
Sbjct: 1   MLNKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120
           REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR
Sbjct: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120

Query: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180
           KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQ+KLKILTAIAEEYNIKWDPK
Sbjct: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQSKLKILTAIAEEYNIKWDPK 180

Query: 181 SFGDSINPPADLLSGXXXXXXXXXXXXXXXXXXXXXDHKESSRKHVPXXXXXXXXXXXXX 240
           SFGDSINPPADLL+G                     D KESSRKHVP XXXXXXXXXXXX
Sbjct: 181 SFGDSINPPADLLNGPNTFGKASQIQMESIGGPSSFDRKESSRKHVPFXXXXXXXXXXXX 240

Query: 241 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQNNYSSGRQQWSM 300
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQNNYSSGRQQWSM
Sbjct: 241 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQNNYSSGRQQWSM 300

Query: 301 DFKDATXXXXXXXXXXXXXXXXXXXXXXXXSRGNISQPSSSEFQKSSSYNLRAEGPQGYP 360
           DFKDATXXXXXXXXXXXXXXXXXXXXXXXXSRGNISQPSSSEFQKSSSYN+RAEGPQGYP
Sbjct: 301 DFKDATXXXXXXXXXXXXXXXXXXXXXXXXSRGNISQPSSSEFQKSSSYNVRAEGPQGYP 360

Query: 361 SVHLRDQQLPKDQVVSAPRKSSTPDDNWRDNETRTYMGDNSKNYSYPSSSVSNNDVNISV 420
           SVHL DQQLPKDQVV APRKSSTPDDNWRDNETR YMGDNSKN SYPSSSVSNNDVN SV
Sbjct: 361 SVHLHDQQLPKDQVVGAPRKSSTPDDNWRDNETRRYMGDNSKNSSYPSSSVSNNDVNTSV 420

Query: 421 TNLSAAERSSFKKSSEPRFSGSLGSSATLEKQ 453
           TN +AAERSSFK SSEPRFSGSLGSSAT+EKQ
Sbjct: 421 TNFNAAERSSFKNSSEPRFSGSLGSSATVEKQ 452

BLAST of CsaV3_UNG052420.1 vs. TrEMBL
Match: tr|A0A2R6QUW0|A0A2R6QUW0_ACTCH (Flocculation protein OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc13023 PE=4 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 7.4e-84
Identity = 158/196 (80.61%), Postives = 184/196 (93.88%), Query Frame = 0

Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           ML +SFKPAKCKTSLKLA SR+KLL+NKK+V +KQLK +LA+LLEAGQDQTARIRVEH V
Sbjct: 1   MLQRSFKPAKCKTSLKLASSRLKLLKNKKEVVVKQLKRELAQLLEAGQDQTARIRVEHVV 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120
           REEK+  A++L+EI+CELIVAR+P+IE+QKNCPIDLKEA++SV+FASPRCADIPEL DVR
Sbjct: 61  REEKTMAAFDLLEIYCELIVARLPIIEAQKNCPIDLKEAITSVVFASPRCADIPELHDVR 120

Query: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180
           KHF +KYGKEF+SAAVELRPECGV+RMLVEKLSAKAPDGQTKLKILTAIAEE+N+KWDPK
Sbjct: 121 KHFTAKYGKEFISAAVELRPECGVSRMLVEKLSAKAPDGQTKLKILTAIAEEHNVKWDPK 180

Query: 181 SFGD-SINPPADLLSG 196
           SFG+  +NPP DLL+G
Sbjct: 181 SFGEKELNPPDDLLNG 196

BLAST of CsaV3_UNG052420.1 vs. TrEMBL
Match: tr|A0A2R6Q506|A0A2R6Q506_ACTCH (Flocculation protein OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc23313 PE=4 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 1.6e-83
Identity = 158/200 (79.00%), Postives = 185/200 (92.50%), Query Frame = 0

Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           ML ++FKPAKCKTSLKLA SR+KLL+NKK+V +KQLK +LA+LLEAGQDQTARIRVEH V
Sbjct: 1   MLHRNFKPAKCKTSLKLASSRLKLLKNKKEVVVKQLKKELAQLLEAGQDQTARIRVEHVV 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120
           REEK++ A++L+EI+CELIVAR+PMIE+QKNCPIDLKEAV+SV+FASPRCADIPEL DVR
Sbjct: 61  REEKTRAAFDLLEIYCELIVARLPMIEAQKNCPIDLKEAVTSVVFASPRCADIPELQDVR 120

Query: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180
           KHF +KYGKEF+SAAVELRPECGV+RMLVEKLSAKAPDGQTKLKILTAIAEE+N+KWDPK
Sbjct: 121 KHFTAKYGKEFISAAVELRPECGVSRMLVEKLSAKAPDGQTKLKILTAIAEEHNVKWDPK 180

Query: 181 SFGDS-----INPPADLLSG 196
           SFG++     + PP DLL+G
Sbjct: 181 SFGENEVEKEVKPPDDLLNG 200

BLAST of CsaV3_UNG052420.1 vs. TrEMBL
Match: tr|A0A2C9UAB7|A0A2C9UAB7_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_16G104700 PE=4 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 2.6e-81
Identity = 201/386 (52.07%), Postives = 247/386 (63.99%), Query Frame = 0

Query: 1   MLSKSFKPAKCKTSLKLAVSRIKLLRNKKDVHIKQLKGDLAKLLEAGQDQTARIRVEHFV 60
           ML KSFKPAKCKTSLKLA SRIKLL+NK+D  +KQLK +LA+LLE+GQDQTARIRVEH V
Sbjct: 1   MLHKSFKPAKCKTSLKLAASRIKLLKNKRDAQVKQLKRELAQLLESGQDQTARIRVEHVV 60

Query: 61  REEKSKEAYELIEIFCELIVARMPMIESQKNCPIDLKEAVSSVIFASPRCADIPELLDVR 120
           REEK+  AY+LIEI+CELIVAR+P+IESQKNCPIDLKEA++S+IFASPRCAD+PEL+DVR
Sbjct: 61  REEKTMAAYDLIEIYCELIVARLPIIESQKNCPIDLKEAIASLIFASPRCADVPELMDVR 120

Query: 121 KHFKSKYGKEFVSAAVELRPECGVNRMLVEKLSAKAPDGQTKLKILTAIAEEYNIKWDPK 180
           KHF +KYGKEFV+AAVELRP+CGV+R+LVEKLSAKAPDG TK+KIL+AIAEE+N+ WDPK
Sbjct: 121 KHFTAKYGKEFVTAAVELRPDCGVSRLLVEKLSAKAPDGPTKIKILSAIAEEHNVTWDPK 180

Query: 181 SFGD-SINPPADLLSG-------XXXXXXXXXXXXXXXXXXXXXDHKESSRKHVPXXXXX 240
           SFG+  + PP DLL+G                            + +  S  +       
Sbjct: 181 SFGEKDMKPPEDLLNGPTTFEHSKVHGESPNIQEPSNSVDRGHPNFRAPSNYNDKHEQHV 240

Query: 241 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQNNYS 300
                                                                  Q+ +S
Sbjct: 241 DSYGYNSRSSQHFQNVSSPAVNTGQAMPSGTSHLDPRTVGTGSNEREFRDSHAAEQSGFS 300

Query: 301 SGRQQWSMDFKDATXXXXXXXXXXXXXXXXXXXXXXXXSRGNISQPSSSEFQKSSSYNLR 360
            GR  W+M+FKDAT XXXXXXXXXXXXXXXXXXXXXX S+G I++  S+E +K+  +  R
Sbjct: 301 LGRNSWNMEFKDATAXXXXXXXXXXXXXXXXXXXXXXSSQGRITRQHSTETKKAPVFRSR 360

Query: 361 AEGPQGYPSVHLRDQQLPKDQVVSAP 379
            EG Q Y    +  +   +D V + P
Sbjct: 361 DEGLQTYAGPRVEGEHNDRDPVNNTP 386

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004135813.12.0e-178100.00PREDICTED: uncharacterized protein LOC101217093 [Cucumis sativus] >KGN66208.1 hy... [more]
XP_008450820.13.5e-17092.04PREDICTED: uncharacterized protein LOC103492291 [Cucumis melo][more]
XP_022927973.15.8e-14969.40filaggrin isoform X1 [Cucurbita moschata][more]
XP_023002515.15.8e-14969.61uncharacterized protein LOC111496330 isoform X1 [Cucurbita maxima][more]
XP_023531863.13.8e-14868.97filaggrin-like isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G19710.12.0e-6663.59Regulator of Vps4 activity in the MVB pathway protein[more]
AT4G29440.15.0e-6262.16Regulator of Vps4 activity in the MVB pathway protein[more]
AT4G35730.18.3e-5757.30Regulator of Vps4 activity in the MVB pathway protein[more]
AT1G34220.14.5e-5550.89Regulator of Vps4 activity in the MVB pathway protein[more]
AT1G25420.11.1e-5351.06Regulator of Vps4 activity in the MVB pathway protein[more]
Match NameE-valueIdentityDescription
sp|Q54I39|IST1L_DICDI5.5e-2636.51IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1[more]
sp|P53990|IST1_HUMAN6.1e-2536.32IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1[more]
sp|Q9CX00|IST1_MOUSE1.4e-2436.81IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1[more]
sp|Q568Z6|IST1_RAT1.4e-2436.81IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1[more]
sp|Q3ZBV1|IST1_BOVIN3.0e-2435.79IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A0A0LWR1|A0A0A0LWR1_CUCSA1.3e-178100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G580230 PE=4 SV=1[more]
tr|A0A1S3BQ24|A0A1S3BQ24_CUCME2.3e-17092.04uncharacterized protein LOC103492291 OS=Cucumis melo OX=3656 GN=LOC103492291 PE=... [more]
tr|A0A2R6QUW0|A0A2R6QUW0_ACTCH7.4e-8480.61Flocculation protein OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_A... [more]
tr|A0A2R6Q506|A0A2R6Q506_ACTCH1.6e-8379.00Flocculation protein OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_A... [more]
tr|A0A2C9UAB7|A0A2C9UAB7_MANES2.6e-8152.07Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_16G104700 PE=4 SV=... [more]
The following terms have been associated with this mRNA:
Vocabulary: Biological Process
TermDefinition
GO:0015031protein transport
Vocabulary: INTERPRO
TermDefinition
IPR005061Ist1
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CsaV3_UNG052420CsaV3_UNG052420gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CsaV3_UNG052420.1.cds7CsaV3_UNG052420.1.cds7CDS
CsaV3_UNG052420.1.cds6CsaV3_UNG052420.1.cds6CDS
CsaV3_UNG052420.1.cds5CsaV3_UNG052420.1.cds5CDS
CsaV3_UNG052420.1.cds4CsaV3_UNG052420.1.cds4CDS
CsaV3_UNG052420.1.cds3CsaV3_UNG052420.1.cds3CDS
CsaV3_UNG052420.1.cds2CsaV3_UNG052420.1.cds2CDS
CsaV3_UNG052420.1.cds1CsaV3_UNG052420.1.cds1CDS


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CsaV3_UNG052420.1.exon7CsaV3_UNG052420.1.exon7exon
CsaV3_UNG052420.1.exon6CsaV3_UNG052420.1.exon6exon
CsaV3_UNG052420.1.exon5CsaV3_UNG052420.1.exon5exon
CsaV3_UNG052420.1.exon4CsaV3_UNG052420.1.exon4exon
CsaV3_UNG052420.1.exon3CsaV3_UNG052420.1.exon3exon
CsaV3_UNG052420.1.exon2CsaV3_UNG052420.1.exon2exon
CsaV3_UNG052420.1.exon1CsaV3_UNG052420.1.exon1exon


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CsaV3_UNG052420.1CsaV3_UNG052420.1-proteinpolypeptide


Analysis Name: InterPro Annotations of cucumber chineselong genome (v3)
Date Performed: 2019-03-04
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005061Vacuolar protein sorting-associated protein Ist1PFAMPF03398Ist1coord: 12..176
e-value: 6.1E-60
score: 201.8
IPR005061Vacuolar protein sorting-associated protein Ist1PANTHERPTHR12161UNCHARACTERIZED DUF292coord: 1..472
NoneNo IPR availableGENE3DG3DSA:1.20.1260.60coord: 1..186
e-value: 4.1E-74
score: 250.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 476..528
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 476..537
NoneNo IPR availablePANTHERPTHR12161:SF13REGULATOR OF VPS4 ACTIVITY PROTEIN-RELATEDcoord: 1..472