CsaV3_1G037510.1 (mRNA) Cucumber (Chinese Long) v3

BLAST of CsaV3_1G037510.1 vs. NCBI nr
Match: XP_004135739.1 (PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 1072.8 bits (2773), Expect = 3.2e-310
Identity = 531/531 (100.00%), Postives = 531/531 (100.00%), Query Frame = 0

Query: 4   ASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILII 63
           ASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILII
Sbjct: 28  ASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILII 87

Query: 64  TPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRR 123
           TPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRR
Sbjct: 88  TPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRR 147

Query: 124 TAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVI 183
           TAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVI
Sbjct: 148 TAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVI 207

Query: 184 DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE 243
           DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE
Sbjct: 208 DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE 267

Query: 244 EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKI 303
           EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKI
Sbjct: 268 EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKI 327

Query: 304 LKEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITSKKVKIKSD 363
           LKEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITSKKVKIKSD
Sbjct: 328 LKEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITSKKVKIKSD 387

Query: 364 YVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIAY 423
           YVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIAY
Sbjct: 388 YVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIAY 447

Query: 424 LTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTNH 483
           LTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTNH
Sbjct: 448 LTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTNH 507

Query: 484 DQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQEDNLVKLV 535
           DQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQEDNLVKLV
Sbjct: 508 DQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQEDNLVKLV 558

BLAST of CsaV3_1G037510.1 vs. NCBI nr
Match: XP_011659204.1 (PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 745.3 bits (1923), Expect = 1.4e-211
Identity = 366/525 (69.71%), Postives = 433/525 (82.48%), Query Frame = 0

Query: 4   ASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILII 63
           +S   HEEFLQCLLHHSPHS SIAKL YT  N+SYSSVLN SIRN RFS PNTPKP+ II
Sbjct: 27  SSHVNHEEFLQCLLHHSPHSKSIAKLAYTPINTSYSSVLNFSIRNLRFSIPNTPKPLFII 86

Query: 64  TPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRR 123
           TP+NISH+QAAVICSKS GLQIRIRSGGHDFEGLSYVAY PFI+VDLI+L S+T++VK+ 
Sbjct: 87  TPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGLSYVAYHPFIIVDLIDLSSVTIEVKQS 146

Query: 124 TAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVI 183
           TAWVQS ATLGELYYRIAEKS TL FP G  PTV  GG+ SGGG+G LLRKYGLAADNVI
Sbjct: 147 TAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTVGVGGHFSGGGFGTLLRKYGLAADNVI 206

Query: 184 DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE 243
           DAYLVDA+G FHDR+SMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVT C+ SRT E
Sbjct: 207 DAYLVDAHGVFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICTISRTLE 266

Query: 244 EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKI 303
           E+AI L+ QWQYV  KL++++  G+  +GG+++++ D K+ P+A+F++ FLGKA++ + I
Sbjct: 267 EEAIKLVDQWQYVANKLDEDLFLGINLLGGKISAQGD-KINPIALFFSLFLGKADELMAI 326

Query: 304 LKEKFPQLGLKKEECKEASWVESVVIAANDFTV-GEPVEALLNRSALIPPITSKKVKIKS 363
           L + FPQLGL KEECKE SW+ESVV   N   +  +P+E LLNR+    P+ +  +K+KS
Sbjct: 327 LNKTFPQLGLTKEECKETSWIESVVYTGNGLQIEDQPLEVLLNRT----PLATGNIKMKS 386

Query: 364 DYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIA 423
           DYVKEP+PK  IE IW R+  SQDI G N++FVPYGGRMS+IS+SEI FSHRAGNL+KI 
Sbjct: 387 DYVKEPIPKATIEEIWQRL-ESQDIEGANLVFVPYGGRMSQISDSEIPFSHRAGNLYKIG 446

Query: 424 YLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTN 483
           YLTGW +P ++ E  HLNWIR+IY YM PFVSKSPR+AYVNYRDLDIGSNS KYG   T+
Sbjct: 447 YLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAYVNYRDLDIGSNS-KYGK--TS 506

Query: 484 HDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQE 528
           + +A  WGLKY+GNNFNR+V +K KVDPYNFFRHEQSIP+ L  +
Sbjct: 507 YKRARVWGLKYFGNNFNRMVYVKNKVDPYNFFRHEQSIPVLLKHK 542

BLAST of CsaV3_1G037510.1 vs. NCBI nr
Match: XP_008445709.1 (PREDICTED: cannabidiolic acid synthase-like [Cucumis melo])

HSP 1 Score: 739.6 bits (1908), Expect = 7.6e-210
Identity = 361/524 (68.89%), Postives = 432/524 (82.44%), Query Frame = 0

Query: 4   ASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILII 63
           +S   HEEFL CLLHHSP S SI KL+YT  N+SYS VLN SIRN RFSTPNTPKP+LII
Sbjct: 27  SSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTSYSPVLNFSIRNLRFSTPNTPKPLLII 86

Query: 64  TPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRR 123
           TPSNISH+QAAVICSKS G+QIRIRSGGHDFEGLSYVAY PF+VVDLINL S+T++V++ 
Sbjct: 87  TPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGLSYVAYHPFVVVDLINLSSVTIEVEQS 146

Query: 124 TAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVI 183
           TAWVQS ATLGELYYRIAEKS TL FP G CPTV  GG+ SGGG+G LLRKYGLAADNVI
Sbjct: 147 TAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTVGVGGHFSGGGFGTLLRKYGLAADNVI 206

Query: 184 DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE 243
           DAY+VDANGEFHDR+SMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVT C+ SRT E
Sbjct: 207 DAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICTISRTLE 266

Query: 244 EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKI 303
           E+AI LI QWQYV  KL++++  G+  +GG++ ++ D K+ P+A+F++ FLGKA++ + I
Sbjct: 267 EEAIKLIDQWQYVANKLDEDLFLGINLLGGKIVAQGD-KINPIALFFSLFLGKADELMAI 326

Query: 304 LKEKFPQLGLKKEECKEASWVESVVIAANDFTV-GEPVEALLNRSALIPPITSKKVKIKS 363
           L + FPQLGL KEECKE SW+ESVV   N   +  +P+E LLNR+    P+ +  +K+KS
Sbjct: 327 LNKTFPQLGLTKEECKETSWIESVVYTGNGLQIEDQPLEVLLNRT----PLANGNIKMKS 386

Query: 364 DYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIA 423
           DYVKEP+ K  I+ +W R+  SQDI G N++F+PYGGRMS+ISESEI FSHRAGNL+KI 
Sbjct: 387 DYVKEPISKATIKEVWQRL-KSQDIEGANLVFIPYGGRMSQISESEIPFSHRAGNLYKIG 446

Query: 424 YLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTN 483
           YLTGW +P ++ E  HLNWIR+IYSYM PFV+KSPR+AYVNYRDLDIGSNS KYG   T+
Sbjct: 447 YLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAAYVNYRDLDIGSNS-KYGK--TS 506

Query: 484 HDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQ 527
           + +A  WG KY+G+NF+RLV +K +VDPYNFFRHEQSIP+ L +
Sbjct: 507 YKRAHVWGSKYFGSNFDRLVYVKNEVDPYNFFRHEQSIPVLLKR 541

BLAST of CsaV3_1G037510.1 vs. NCBI nr
Match: XP_011659211.1 (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 729.6 bits (1882), Expect = 7.9e-207
Identity = 359/521 (68.91%), Postives = 425/521 (81.57%), Query Frame = 0

Query: 4   ASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILII 63
           +SS  HEEF+QCLLHHSP +  I KL++T  NSS+SS+LN   RN RFSTPNTPKP+LII
Sbjct: 27  SSSINHEEFIQCLLHHSPDTYLITKLVHTPINSSFSSLLNFPTRNSRFSTPNTPKPLLII 86

Query: 64  TPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRR 123
           TPSNISHIQAAV+CSKS GLQIRIRSGGHDFEGLSYVAY  FIVVDLINL+S+TVDV++ 
Sbjct: 87  TPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGLSYVAYHQFIVVDLINLKSVTVDVEQS 146

Query: 124 TAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVI 183
           TAWV+S ATLGELYY+I +KS TL FP G CPTV  GG+ SGGGYG LLRKYG+AADNVI
Sbjct: 147 TAWVESGATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSGGGYGSLLRKYGVAADNVI 206

Query: 184 DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE 243
           DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVT CS+ R+ E
Sbjct: 207 DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICSAKRSLE 266

Query: 244 EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKI 303
           EDAI LI QWQYV  KLE+ +   +   GG+++++  GK+ P+A+F+T FLG  N+ + I
Sbjct: 267 EDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSAQEGGKINPIALFFTLFLGNVNELMAI 326

Query: 304 LKEKFPQLGLKKEECKEASWVESVVIAANDFTV-GEPVEALLNRSALIPPITSKKVKIKS 363
           L + FP+LGL KEECKE SW+ES   AAN F +  +P+EALLNR+    PIT    K+KS
Sbjct: 327 LSKTFPELGLTKEECKEKSWIESAASAANRFQIEDQPLEALLNRT----PITHGSFKLKS 386

Query: 364 DYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIA 423
           DYVKEPM K AI+GIW R+  SQDI G+ +  +PYGGRM++ISES+I F HRAG L++I 
Sbjct: 387 DYVKEPMTKAAIQGIWKRL-ESQDIEGVTLAVIPYGGRMNQISESKIPFPHRAGILYQIG 446

Query: 424 YLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTN 483
           Y+ GWE+  ++ E RHLNWIREIYSYM PFVSKSPR+AYVNYRDLDIGSN +KYG I  N
Sbjct: 447 YILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAYVNYRDLDIGSN-NKYGKI--N 506

Query: 484 HDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIA 524
           + QA  +G KY+GNNFNRLV++K+ VDPYNFF HEQSIP++
Sbjct: 507 YKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPLS 539

BLAST of CsaV3_1G037510.1 vs. NCBI nr
Match: XP_023000935.1 (berberine bridge enzyme-like 27 [Cucurbita maxima])

HSP 1 Score: 724.9 bits (1870), Expect = 1.9e-205
Identity = 356/530 (67.17%), Postives = 428/530 (80.75%), Query Frame = 0

Query: 5   SSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILIIT 64
           SS+++++FLQCLLHHSP + SI KL+YT  N+SYSS+LN SI+N RFSTP TPKP+ IIT
Sbjct: 26  SSNQNKDFLQCLLHHSPDTHSITKLVYTPINASYSSLLNFSIKNLRFSTPKTPKPLAIIT 85

Query: 65  PSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRRT 124
           PSNI HIQAA+ICSKS GLQIRIRSGGHD EGLSYVAY+PFIVVDLINL  + VDV++ T
Sbjct: 86  PSNIHHIQAAIICSKSHGLQIRIRSGGHDLEGLSYVAYVPFIVVDLINLSLVEVDVEKNT 145

Query: 125 AWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVID 184
           AWVQS A+LGELYYRIAEKS TL FP G CPTV  GG  SGGGYG+L+RKYGLAADNV+D
Sbjct: 146 AWVQSGASLGELYYRIAEKSRTLAFPAGLCPTVGVGGQFSGGGYGMLMRKYGLAADNVVD 205

Query: 185 AYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFEE 244
           AYLVDANGEF DRE MGE+LFWAIRGGGGGSFG+VVAWKVKLVPVPATVT C++S++  +
Sbjct: 206 AYLVDANGEFFDREGMGEELFWAIRGGGGGSFGVVVAWKVKLVPVPATVTVCATSKSLVD 265

Query: 245 DAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKIL 304
            AINLI +WQYV  KL++N+  G++  GG       GK  P+AIF++ FLGKA++ V IL
Sbjct: 266 GAINLIDKWQYVADKLDENLFLGIILAGG-------GKTNPLAIFFSLFLGKADELVTIL 325

Query: 305 KEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITSKKVKIKSDY 364
              FP+LGL KE+C E SW+E+ +IAAN F  GE +EALLNRSALIPPI S + K KSDY
Sbjct: 326 NNPFPELGLSKEDCIETSWIEAALIAANGFQTGEVLEALLNRSALIPPINSGRSKTKSDY 385

Query: 365 VKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIAYL 424
           VKEPM + AIEG+WNR + SQ+I   +++F+PYGG+MSEISESEI F HRAGN ++I Y+
Sbjct: 386 VKEPMTRTAIEGMWNR-SKSQEIERASLVFIPYGGKMSEISESEIPFPHRAGNRYEIDYI 445

Query: 425 TGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTNHD 484
            GW++  +  E RHLNWIRE+YSYM PFVSKSPR+ YVN RDLDIGSN D YG   T++ 
Sbjct: 446 VGWDEQGVHAEKRHLNWIRELYSYMTPFVSKSPRAVYVNCRDLDIGSN-DMYGR--TSYK 505

Query: 485 QASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQEDNLVKLV 535
           +A  WGLKY+GNNF+RLV +KT+VDPY+FFRHEQSIP  LS      K++
Sbjct: 506 RARVWGLKYFGNNFDRLVHVKTEVDPYDFFRHEQSIP-TLSPTKEFSKII 543

BLAST of CsaV3_1G037510.1 vs. TAIR10
Match: AT5G44440.1 (FAD-binding Berberine family protein)

HSP 1 Score: 486.1 bits (1250), Expect = 2.8e-137
Identity = 245/526 (46.58%), Postives = 346/526 (65.78%), Query Frame = 0

Query: 1   MTKASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPI 60
           +  A    HE+FL+CL +    ++   K+I+T  +SS+ S+L+ SI+N RFS   TPKP+
Sbjct: 20  LVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSIQNPRFSVSETPKPV 79

Query: 61  LIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAY-LPFIVVDLINLRSITVD 120
            IITP   S +Q  + C++  G+ +R RS GH +EGLSY+AY  PF V+DL NLRSI++D
Sbjct: 80  SIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVIDLRNLRSISLD 139

Query: 121 VKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAA 180
           V  RT WVQ+ AT GELYY I + + +L FP G  PTV  GG  SGGGYG LLRKYGLAA
Sbjct: 140 VDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGYGTLLRKYGLAA 199

Query: 181 DNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSS 240
           DN+IDA +VDA+G   DR++MGED FWAIRGGGG SFG++++WKVKLV VP+T+T     
Sbjct: 200 DNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVPSTITVFKVQ 259

Query: 241 RTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANK 300
           +T +++A+ +I +WQY   K+  ++         + T E   K    A+F   ++G  N 
Sbjct: 260 KTSKKEAVRIIKKWQYAADKVPDDLFI-------RTTLERSNKNAVHALFTGLYIGPVNN 319

Query: 301 AVKILKEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITSKKVK 360
            + +++EKFP+LGL+KE C+E SW+ESV+  A DF  GE +  L NR       TS   K
Sbjct: 320 LLALMEEKFPELGLEKEGCEEMSWIESVLWFA-DFPKGESLGVLTNRER-----TSLSFK 379

Query: 361 IKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLF 420
            K D+V+EP+P+ AI+ IW R+   +   G  ++  P+GG+MSE++E E  F HR GNL+
Sbjct: 380 GKDDFVQEPIPEAAIQEIWRRLEAPEARLG-KIILTPFGGKMSEMAEYETPFPHRGGNLY 439

Query: 421 KIAYLTGW---EDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKY 480
           +I Y+  W   ED +     ++L W+  +Y +M P+VSKSPR AYVN++D+D+G      
Sbjct: 440 EIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDLGM---YL 499

Query: 481 GNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPI 523
           G   T +++  SWG+KY+ NNF RLV++KT+VDP +FF  EQSIP+
Sbjct: 500 GKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIPL 528

BLAST of CsaV3_1G037510.1 vs. TAIR10
Match: AT5G44410.1 (FAD-binding Berberine family protein)

HSP 1 Score: 483.4 bits (1243), Expect = 1.8e-136
Identity = 246/523 (47.04%), Postives = 344/523 (65.77%), Query Frame = 0

Query: 4   ASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILII 63
           A    HE FL+CL H      S  ++I+T  + SY S+LN SI+N RF    TPKP+ II
Sbjct: 29  AQRTNHENFLKCLSHRINEDDS--RIIHTSKDPSYFSILNSSIQNPRFFVLETPKPVSII 88

Query: 64  TPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVA-YLPFIVVDLINLRSITVDVKR 123
           TP   + +Q+ + C++  G+ IR RSGGHD+EGLSY+A   PF+V+DL NLRSIT+DV  
Sbjct: 89  TPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDVDN 148

Query: 124 RTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNV 183
           RT WVQS AT+GELYY I + S +L FP G  PTV  GG   GGGYG L+RKYGL+ADNV
Sbjct: 149 RTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSADNV 208

Query: 184 IDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTF 243
           IDA++VDANG F DR+ MGED FWAIRGGGG SF +V++WK++L+ VP+ VT     +T 
Sbjct: 209 IDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVKTS 268

Query: 244 EEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVK 303
           E++A+++I++WQY+  K+  +     L I   +  E +      A F   +LG  +  + 
Sbjct: 269 EKEAVSIINKWQYIADKVPND-----LFIRAMLQKETE----VYASFPGLYLGPVSDLLA 328

Query: 304 ILKEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITSKKVKIKS 363
           ++K+KFP+LGL+   C+E SW+ESV+     F  GE +E L  R       TS+  K K 
Sbjct: 329 LMKDKFPELGLEIGNCREMSWIESVLW----FIKGESMEILAKRKR-----TSRSFKGKD 388

Query: 364 DYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIA 423
           D+++EP+PK AI+ +W R   + +     ++  P+GG+MSEI+++EI F HR GNL++I 
Sbjct: 389 DFIEEPIPKTAIQYLWRRF-EAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQ 448

Query: 424 YLTGW---EDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNI 483
           YL  W   ED +     ++L W+  +Y +M P+VSKSPR AYVN+RD+D+G       N+
Sbjct: 449 YLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGL--NM 508

Query: 484 VTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPI 523
            T +++A  WG+KY+ NNF+RLV++KT VDP +FF  EQSIPI
Sbjct: 509 KTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIPI 528

BLAST of CsaV3_1G037510.1 vs. TAIR10
Match: AT4G20820.1 (FAD-binding Berberine family protein)

HSP 1 Score: 481.1 bits (1237), Expect = 8.9e-136
Identity = 244/518 (47.10%), Postives = 343/518 (66.22%), Query Frame = 0

Query: 4   ASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILII 63
           A+      FLQCL      S+ ++K+I+T N++S+SSVL  SI+N RFS P+ PKP+LI+
Sbjct: 29  ANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLIL 88

Query: 64  TPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRR 123
           TP   S +Q+AV C++  G+ IR RSGGHD+EGLSYV + PF+++DL NLRSITVDV  R
Sbjct: 89  TPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDNR 148

Query: 124 TAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVI 183
           + WVQ+ AT+GELYY I +K+ TL FP G CPTV  GG+ SGGGYG LLRK+GLAAD+VI
Sbjct: 149 SVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHVI 208

Query: 184 DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE 243
           DA +VDA G   +R  MGED FWAIRGGGG SF +V++WK+ L+ VP+TVT  + ++  E
Sbjct: 209 DARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFSE 268

Query: 244 EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKI 303
           + A+ +IH+WQ+V  K+  ++   V      M       V+  A F   +LG     +K+
Sbjct: 269 QSALKIIHRWQFVADKVSDDLFIRV------MLQRYKNMVR--ASFPGLYLGSVKNLLKM 328

Query: 304 LKEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITSKKVKIKSD 363
           + ++FP+LGL++++C E SW+ESV+  A      EP+  L  R+       S   K KSD
Sbjct: 329 VNKEFPELGLEEDDCTEMSWIESVIWFAE--LGEEPINVLTKRTR-----ASLAFKAKSD 388

Query: 364 YVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIAY 423
           +V+EPMPK AI  +W R+    +     ++F P+GG+MSEI++ E  F HR GN+++I Y
Sbjct: 389 FVQEPMPKTAISKLWRRL-QEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQY 448

Query: 424 LTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTNH 483
           L  W     DV+ +++ W+  +Y  M+ FV+KSPR AY+N RDLD+G      G   + +
Sbjct: 449 LNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGM---YVGVKRSKY 508

Query: 484 DQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIP 522
           ++  SWG+KY+ NNF RLV++KT VDP +FF  EQSIP
Sbjct: 509 EEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of CsaV3_1G037510.1 vs. TAIR10
Match: AT2G34790.1 (FAD-binding Berberine family protein)

HSP 1 Score: 475.3 bits (1222), Expect = 4.9e-134
Identity = 240/522 (45.98%), Postives = 341/522 (65.33%), Query Frame = 0

Query: 4   ASSDKHEEFLQCLLHHSPHSSSIAKLIYT--QNNSSYSSVLNLSIRNHRFSTPNTPKPIL 63
           +SS   ++F++CL+ +S  S  I    ++  QN + +   L  + +N R+ TP+ PKP+ 
Sbjct: 25  SSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTAQNLRYLTPSNPKPVF 84

Query: 64  IITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVA--YLPFIVVDLINLRSITVD 123
           I  P   +H+QAAV+C+K   L +R+RSGGHD+EGLS+VA    PF++VDL  LR + VD
Sbjct: 85  IFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPFVIVDLSKLRQVDVD 144

Query: 124 VKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAA 183
           +   +AW  + AT+GE+YYRI EKS T  FP G C ++  GG+L GG YG ++RK+GL A
Sbjct: 145 LDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGGAYGSMMRKFGLGA 204

Query: 184 DNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSS 243
           DNV+DA +VDANG+  DR +MGED+FWAIRGGGGGSFG+++AWK+KLVPVPATVT  + +
Sbjct: 205 DNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPATVTVFTVT 264

Query: 244 RTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANK 303
           +T E+D   ++++W+ +  KL+ ++   V+      T++   +   ++ +   FLG +N+
Sbjct: 265 KTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTISMS-YQAQFLGDSNR 324

Query: 304 AVKILKEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITSKKVK 363
            ++++++ FP+LGL K++C E SW++SV+  A  F      EALL   +L         K
Sbjct: 325 LLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAG-FPNSAAPEALLAGKSLF----KNHFK 384

Query: 364 IKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLF 423
            KSD+VKEP+P   +EG+W R            ++ PYGG MS ISESEI F HR G LF
Sbjct: 385 AKSDFVKEPIPVEGLEGLWERFLEED---SPLTIWNPYGGMMSRISESEIPFPHRNGTLF 444

Query: 424 KIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNI 483
           KI +L+ W+D  +  E RH+ WIRE+YSYM  +VSK+PR AYVNYRDLD+G+N       
Sbjct: 445 KIQWLSTWQDGKVS-EERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNE------ 504

Query: 484 VTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIP 522
                 A  WG KYY  NF RLV+IK + DP NFFRHEQS+P
Sbjct: 505 --GETDAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVP 528

BLAST of CsaV3_1G037510.1 vs. TAIR10
Match: AT5G44380.1 (FAD-binding Berberine family protein)

HSP 1 Score: 474.2 bits (1219), Expect = 1.1e-133
Identity = 252/527 (47.82%), Postives = 341/527 (64.71%), Query Frame = 0

Query: 2   TKASSDKHEEFLQCLLHHSPHSSSIAKLIYT--QNNSSYSSVLNLSIRNHRFSTPNTPKP 61
           T +S+   ++F+ C+  ++  S  + K ++T  +N S ++ VL  + +N +F   + PKP
Sbjct: 28  TSSSASIQDQFINCVKRNTHVSFPLEKTLFTPAKNVSLFNQVLESTAQNLQFLAKSMPKP 87

Query: 62  ILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYL--PFIVVDLINLRSIT 121
             I  P + S +QA++ICSK  G+  R+RSGGHDFE LSYV+ +  PFI++DL  L+ I 
Sbjct: 88  GFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEALSYVSRIEKPFILLDLSKLKQIN 147

Query: 122 VDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGL 181
           VD++  +AWVQ  ATLGELYYRIAEKS    FP G C +V  GGY++GGGYG L+RKYGL
Sbjct: 148 VDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLCTSVGIGGYMTGGGYGTLMRKYGL 207

Query: 182 AADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCS 241
           A DNV+D  +VDANG+  DR +MGEDLFWAIRGGGG SFGIV+AWK+KLVPVP TVT  +
Sbjct: 208 AGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLAWKIKLVPVPKTVTVFT 267

Query: 242 SSRTFEEDA-INLIHQWQYVGYKLEKNI-AHGVLTIGGQMTSEVDGKVKPVAIFYTSFLG 301
            ++T E+DA +  I +WQ +  K+ + I    VL   G      DG       +   FLG
Sbjct: 268 VTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLRAAGN-----DGNKTVTMTYLGQFLG 327

Query: 302 KANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITS 361
           +    +K++++ FP+LGL +++C E SW+E+ +     F  G P+E LL    L  P+  
Sbjct: 328 EKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGG-FPTGSPIEILLQ---LKSPLGK 387

Query: 362 KKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVL-FVPYGGRMSEISESEISFSHR 421
              K  SD+VKEP+P + ++GI+ R+      G    L + PYGG MS+I ES I F HR
Sbjct: 388 DYFKATSDFVKEPIPVIGLKGIFKRLIE----GNTTFLNWTPYGGMMSKIPESAIPFPHR 447

Query: 422 AGNLFKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSD 481
            G LFKI Y   W + +    +R +NWI+EIY+YMAP+VS +PR AYVNYRDLD G N +
Sbjct: 448 NGTLFKILYYANWLE-NDKTSSRKINWIKEIYNYMAPYVSSNPRQAYVNYRDLDFGQNKN 507

Query: 482 KYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIP 522
              N   N  +A  WG KY+  NF+RLV+IKTKVDP NFFRHEQSIP
Sbjct: 508 ---NAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIP 537

BLAST of CsaV3_1G037510.1 vs. Swiss-Prot
Match: sp|A6P6V9|CBDAS_CANSA (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 3.3e-140
Identity = 250/520 (48.08%), Postives = 350/520 (67.31%), Query Frame = 0

Query: 6   SDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILIITP 65
           ++  E FL+C   + P++++  KL+YTQNN  Y SVLN +I N RF++  TPKP++I+TP
Sbjct: 28  ANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPLVIVTP 87

Query: 66  SNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRRTA 125
           S++SHIQ  ++CSK  GLQIR RSGGHD EG+SY++ +PF++VDL N+RSI +DV  +TA
Sbjct: 88  SHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDVHSQTA 147

Query: 126 WVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVIDA 185
           WV++ ATLGE+YY + EK+  L+   G CPTVC GG+  GGGYG L+R YGLAADN+IDA
Sbjct: 148 WVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPLMRNYGLAADNIIDA 207

Query: 186 YLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFEED 245
           +LV+ +G+  DR+SMGEDLFWA+RGGG  SFGI+VAWK++LV VP +  F         +
Sbjct: 208 HLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVPKSTMFSVKKIMEIHE 267

Query: 246 AINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTS-FLGKANKAVKIL 305
            + L+++WQ + YK +K++      I   +T         +  +++S FLG  +  V ++
Sbjct: 268 LVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQGKNKTAIHTYFSSVFLGGVDSLVDLM 327

Query: 306 KEKFPQLGLKKEECKEASWVESVVI---AANDFTVGEPVEALLNRSALIPPITSKKVKIK 365
            + FP+LG+KK +C++ SW+++++      N  T     E LL+RSA      +   KIK
Sbjct: 328 NKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDTDNFNKEILLDRSA----GQNGAFKIK 387

Query: 366 SDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKI 425
            DYVK+P+P+     I  ++   +DIG       PYGG M EISES I F HRAG L+++
Sbjct: 388 LDYVKKPIPESVFVQILEKL-YEEDIGAGMYALYPYGGIMDEISESAIPFPHRAGILYEL 447

Query: 426 AYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVT 485
            Y+  WE    +   +HLNWIR IY++M P+VSK+PR AY+NYRDLDIG N  K  N   
Sbjct: 448 WYICSWE--KQEDNEKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIGINDPKNPN--- 507

Query: 486 NHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIP 522
           N+ QA  WG KY+G NF+RLV++KT VDP NFFR+EQSIP
Sbjct: 508 NYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIP 537

BLAST of CsaV3_1G037510.1 vs. Swiss-Prot
Match: sp|Q8GTB6|THCAS_CANSA (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 PE=1 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 7.4e-140
Identity = 256/524 (48.85%), Postives = 351/524 (66.98%), Query Frame = 0

Query: 6   SDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILIITP 65
           ++  E FL+C   H P++ +  KL+YTQ++  Y S+LN +I+N RF +  TPKP++I+TP
Sbjct: 28  ANPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTP 87

Query: 66  SNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRRTA 125
           SN SHIQA ++CSK  GLQIR RSGGHD EG+SY++ +PF+VVDL N+ SI +DV  +TA
Sbjct: 88  SNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTA 147

Query: 126 WVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVIDA 185
           WV++ ATLGE+YY I EK+  L+FPGG CPTV  GG+ SGGGYG L+R YGLAADN+IDA
Sbjct: 148 WVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDA 207

Query: 186 YLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE-E 245
           +LV+ +G+  DR+SMGEDLFWAIRGGGG +FGI+ AWK+KLV VP+  T  S  +  E  
Sbjct: 208 HLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIH 267

Query: 246 DAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTS--FLGKANKAVK 305
             + L ++WQ + YK +K++      I   +T    GK K     Y S  F G  +  V 
Sbjct: 268 GLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDN-HGKNKTTVHGYFSSIFHGGVDSLVD 327

Query: 306 ILKEKFPQLGLKKEECKEASWVESVVI---AANDFTVGEPVEALLNRSALIPPITSKKV- 365
           ++ + FP+LG+KK +CKE SW+++ +      N  T     E LL+RSA       KK  
Sbjct: 328 LMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRSA------GKKTA 387

Query: 366 -KIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGN 425
             IK DYVK+P+P+ A+  I  ++   +D+G    +  PYGG M EISES I F HRAG 
Sbjct: 388 FSIKLDYVKKPIPETAMVKILEKL-YEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGI 447

Query: 426 LFKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYG 485
           ++++ Y   WE    +   +H+NW+R +Y++  P+VS++PR AY+NYRDLD+G  +    
Sbjct: 448 MYELWYTASWE--KQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASP 507

Query: 486 NIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIP 522
           N   N+ QA  WG KY+G NFNRLV++KTKVDP NFFR+EQSIP
Sbjct: 508 N---NYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIP 538

BLAST of CsaV3_1G037510.1 vs. Swiss-Prot
Match: sp|A6P6W1|CASL2_CANSA (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 6.9e-138
Identity = 248/522 (47.51%), Postives = 350/522 (67.05%), Query Frame = 0

Query: 6   SDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILIITP 65
           ++  E FL+CL  + P + + AKL+YTQ++  Y S+LN +++N RF++  TPKP++I TP
Sbjct: 28  ANPQENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTP 87

Query: 66  SNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRRTA 125
            N+SHIQ  ++CSK  GLQIR RSGGHD EG+SY++ +PF++VDL N+ S+ +DV  +TA
Sbjct: 88  LNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTA 147

Query: 126 WVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVIDA 185
           WV+S ATLGE+YY I E +  L+FP G CPTV  GG+ SGGGYG L+R YGLAADN+IDA
Sbjct: 148 WVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDA 207

Query: 186 YLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE-E 245
           +LV+ +G+  DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  T  S  +  E  
Sbjct: 208 HLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIH 267

Query: 246 DAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPV--AIFYTSFLGKANKAVK 305
           + + L+++WQ + Y  EK +      I   +T    GK K    + F + F G  +  V 
Sbjct: 268 ELVKLVNKWQNIAYMYEKELLLFTHFITRNITDN-QGKNKTTIHSYFSSIFHGGVDSLVD 327

Query: 306 ILKEKFPQLGLKKEECKEASWVESVVI---AANDFTVGEPVEALLNRSALIPPITSKKVK 365
           ++ + FP+LG+KK +CK+ SW+++++      N  T     E LL+RS            
Sbjct: 328 LMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLDRSG----GRKAAFS 387

Query: 366 IKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLF 425
           IK DYVK+P+P+ A+  I  ++   +D+G    +F PYGG M EISES I F HRAG  +
Sbjct: 388 IKLDYVKKPIPETAMVTILEKL-YEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITY 447

Query: 426 KIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNI 485
           +I Y+  WE    +   +H+NWIR +Y++  P+VS++PR AY+NYRDLD+G  + +  N 
Sbjct: 448 EIWYIASWE--KQEDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPN- 507

Query: 486 VTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIP 522
             N+ QA  WG KY+G NFNRLV++KTKVDP NFFR+EQSIP
Sbjct: 508 --NYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIP 538

BLAST of CsaV3_1G037510.1 vs. Swiss-Prot
Match: sp|A6P6W0|CASL1_CANSA (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 9.1e-138
Identity = 248/522 (47.51%), Postives = 351/522 (67.24%), Query Frame = 0

Query: 6   SDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILIITP 65
           ++  E FL+C   + P + + AKL+YTQ++  Y S+LN +I+N RF++  TPKP++IITP
Sbjct: 28  ANPQENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITP 87

Query: 66  SNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRRTA 125
            N+SHIQ  ++CSK  GLQIR RSGGHD EG+SY++ +PF++VDL N+ S+ +DV  +TA
Sbjct: 88  LNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTA 147

Query: 126 WVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVIDA 185
           WV++ ATLGE+YY I E +  L+FP G CPTV  GG+ SGGGYG L+R YGLAADN+IDA
Sbjct: 148 WVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDA 207

Query: 186 YLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE-E 245
           +LV+ +G+  DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  T  S  +  E  
Sbjct: 208 HLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIH 267

Query: 246 DAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPV--AIFYTSFLGKANKAVK 305
           + + L+++WQ + Y  EK +      I   +T    GK K    + F + F G  +  V 
Sbjct: 268 ELVKLVNKWQNIAYMYEKELLLFTHFITRNITDN-QGKNKTTIHSYFSSIFHGGVDSLVD 327

Query: 306 ILKEKFPQLGLKKEECKEASWVESVVI---AANDFTVGEPVEALLNRSALIPPITSKKVK 365
           ++ + FP+LG+KK +CK+ SW+++++      N  T     E LL+RS            
Sbjct: 328 LMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDRSG----GRKAAFS 387

Query: 366 IKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLF 425
           IK DYVK+P+P+ A+  I  ++   +D+G    +F PYGG M EISES I F HRAG ++
Sbjct: 388 IKLDYVKKPIPETAMVTILEKL-YEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMY 447

Query: 426 KIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNI 485
           +I Y+  WE    +   +H+NWIR +Y++  P+VS++PR AY+NYRDLD+G  + +  N 
Sbjct: 448 EIWYIASWE--KQEDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPN- 507

Query: 486 VTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIP 522
             N+ QA  WG KY+G NFNRLV++KTKVDP NFFR+EQSIP
Sbjct: 508 --NYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIP 538

BLAST of CsaV3_1G037510.1 vs. Swiss-Prot
Match: sp|Q33DQ2|THCAI_CANSA (Inactive tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 PE=3 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 3.4e-137
Identity = 253/526 (48.10%), Postives = 354/526 (67.30%), Query Frame = 0

Query: 6   SDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILIITP 65
           ++  E FL+C   + P++ +  K IYTQ++  Y SVLN +I+N RF++  TPKP++I+TP
Sbjct: 28  ANPQENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRFTSDTTPKPLVIVTP 87

Query: 66  SNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRRTA 125
           SN+SHIQA+++CSK  GLQIR RSGGHD EGLSY++ +PF +VDL N+ ++ VD+  +TA
Sbjct: 88  SNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHTVKVDIHSQTA 147

Query: 126 WVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVIDA 185
           WV++ ATLGE+YY I E +   +FPGG CPTV  GG+ SGGGYG L+R YGLAADN+IDA
Sbjct: 148 WVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDA 207

Query: 186 YLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE-E 245
           +LV+ +G+  DR+SMGEDLFWAIRGGGG +FGI+ AWK+KLV VP+  T  S  +  E  
Sbjct: 208 HLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPSKATIFSVKKNMEIH 267

Query: 246 DAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVD--GKVKPVAIFYTS--FLGKANKA 305
             + L ++WQ + YK +K++   +LT   +  +  D  GK K     Y S  FLG  +  
Sbjct: 268 GLVKLFNKWQNIAYKYDKDL---MLTTHFRTRNITDNHGKNKTTVHGYFSSIFLGGVDSL 327

Query: 306 VKILKEKFPQLGLKKEECKEASWVESVVI---AANDFTVGEPVEALLNRSALIPPITSKK 365
           V ++ + FP+LG+KK +CKE SW+++ +      N  T     E LL+RSA       KK
Sbjct: 328 VDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEILLDRSA------GKK 387

Query: 366 V--KIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRA 425
               IK DYVK+ +P+ A+  I  ++   +   G+ VL+ PYGG M EISES I F HRA
Sbjct: 388 TAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLY-PYGGIMDEISESAIPFPHRA 447

Query: 426 GNLFKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDK 485
           G ++++ Y   WE    +   +H+NW+R +Y++  P+VS++PR AY+NYRDLD+G  + +
Sbjct: 448 GIMYELWYTATWE--KQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNPE 507

Query: 486 YGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIP 522
             N   N+ QA  WG KY+G NFNRLV++KTK DP NFFR+EQSIP
Sbjct: 508 SPN---NYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIP 538

BLAST of CsaV3_1G037510.1 vs. TrEMBL
Match: tr|A0A1S3BCV7|A0A1S3BCV7_CUCME (cannabidiolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488658 PE=3 SV=1)

HSP 1 Score: 739.6 bits (1908), Expect = 5.1e-210
Identity = 361/524 (68.89%), Postives = 432/524 (82.44%), Query Frame = 0

Query: 4   ASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILII 63
           +S   HEEFL CLLHHSP S SI KL+YT  N+SYS VLN SIRN RFSTPNTPKP+LII
Sbjct: 27  SSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTSYSPVLNFSIRNLRFSTPNTPKPLLII 86

Query: 64  TPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRR 123
           TPSNISH+QAAVICSKS G+QIRIRSGGHDFEGLSYVAY PF+VVDLINL S+T++V++ 
Sbjct: 87  TPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGLSYVAYHPFVVVDLINLSSVTIEVEQS 146

Query: 124 TAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVI 183
           TAWVQS ATLGELYYRIAEKS TL FP G CPTV  GG+ SGGG+G LLRKYGLAADNVI
Sbjct: 147 TAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTVGVGGHFSGGGFGTLLRKYGLAADNVI 206

Query: 184 DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE 243
           DAY+VDANGEFHDR+SMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVT C+ SRT E
Sbjct: 207 DAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICTISRTLE 266

Query: 244 EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKI 303
           E+AI LI QWQYV  KL++++  G+  +GG++ ++ D K+ P+A+F++ FLGKA++ + I
Sbjct: 267 EEAIKLIDQWQYVANKLDEDLFLGINLLGGKIVAQGD-KINPIALFFSLFLGKADELMAI 326

Query: 304 LKEKFPQLGLKKEECKEASWVESVVIAANDFTV-GEPVEALLNRSALIPPITSKKVKIKS 363
           L + FPQLGL KEECKE SW+ESVV   N   +  +P+E LLNR+    P+ +  +K+KS
Sbjct: 327 LNKTFPQLGLTKEECKETSWIESVVYTGNGLQIEDQPLEVLLNRT----PLANGNIKMKS 386

Query: 364 DYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIA 423
           DYVKEP+ K  I+ +W R+  SQDI G N++F+PYGGRMS+ISESEI FSHRAGNL+KI 
Sbjct: 387 DYVKEPISKATIKEVWQRL-KSQDIEGANLVFIPYGGRMSQISESEIPFSHRAGNLYKIG 446

Query: 424 YLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTN 483
           YLTGW +P ++ E  HLNWIR+IYSYM PFV+KSPR+AYVNYRDLDIGSNS KYG   T+
Sbjct: 447 YLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAAYVNYRDLDIGSNS-KYGK--TS 506

Query: 484 HDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQ 527
           + +A  WG KY+G+NF+RLV +K +VDPYNFFRHEQSIP+ L +
Sbjct: 507 YKRAHVWGSKYFGSNFDRLVYVKNEVDPYNFFRHEQSIPVLLKR 541

BLAST of CsaV3_1G037510.1 vs. TrEMBL
Match: tr|A0A1S3BDD9|A0A1S3BDD9_CUCME (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488665 PE=3 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 3.7e-205
Identity = 359/521 (68.91%), Postives = 422/521 (81.00%), Query Frame = 0

Query: 4   ASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILII 63
           +SS KHEEFLQCLLHHSP +SSI KL++T  N   S++ N   RN RFSTPNTPKP+LII
Sbjct: 40  SSSIKHEEFLQCLLHHSPDTSSINKLVHTPINXXXSTLFNFPTRNSRFSTPNTPKPLLII 99

Query: 64  TPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRR 123
           TPSNISHIQAAVICSKS GLQIRIRSGGHDFEGLSYVAY  FIVVDLINLRS+TVDV++ 
Sbjct: 100 TPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLSYVAYHRFIVVDLINLRSVTVDVEQS 159

Query: 124 TAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVI 183
           TAWVQS ATLGELYY+I +KS TL FP G CPTV  GG+ SGGGYG LLRKYG+AADNVI
Sbjct: 160 TAWVQSGATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSGGGYGSLLRKYGVAADNVI 219

Query: 184 DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE 243
           DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG+VVAWKVKLVPVPA+VT CS+ R+ E
Sbjct: 220 DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPASVTICSAKRSVE 279

Query: 244 EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKI 303
           E+AI LI +WQYV  KLE+++   +L  GG+++++  GK+ P+A+F+  FLG  N+ + I
Sbjct: 280 EEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSAQGGGKINPIALFFPLFLGNVNELMAI 339

Query: 304 LKEKFPQLGLKKEECKEASWVESVVIAANDFTV-GEPVEALLNRSALIPPITSKKVKIKS 363
           L + FP+LG  KEECKE SW+ES   AAN F +  +P+EALLNR+   P       K+KS
Sbjct: 340 LNKTFPELGFTKEECKEKSWIESAASAANRFQIEDQPLEALLNRT---PINHGSSFKLKS 399

Query: 364 DYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIA 423
           DYVKEPM K AIEGIW R+  SQDI G+ +  +PYGGRMS ISES I FSHRAG L++I 
Sbjct: 400 DYVKEPMTKTAIEGIWKRL-ESQDIEGVTLALIPYGGRMSRISESNIPFSHRAGILYQIG 459

Query: 424 YLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTN 483
           Y+ GW++  ++ E RHLNWIREIYSYM PFVSKSPR+AYVNYRDLDIGSN +KYG I  N
Sbjct: 460 YILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTAYVNYRDLDIGSN-NKYGKI--N 519

Query: 484 HDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIA 524
           + QA  +G KY+GNNFNRLV++KTKVDPYNFF HEQSIP++
Sbjct: 520 YKQACVFGSKYFGNNFNRLVKVKTKVDPYNFFWHEQSIPLS 553

BLAST of CsaV3_1G037510.1 vs. TrEMBL
Match: tr|A0A1S3BDA8|A0A1S3BDA8_CUCME (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488637 PE=3 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 6.2e-192
Identity = 341/518 (65.83%), Postives = 405/518 (78.19%), Query Frame = 0

Query: 4   ASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILII 63
           AS  K++ FLQCL  HS  S  I+K++YT  NSSYSSVL+ SIRN  FS P TPKP+LII
Sbjct: 26  ASPQKYQNFLQCLSEHSSKSYPISKVVYTPINSSYSSVLDFSIRNLLFSKPETPKPLLII 85

Query: 64  TPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRR 123
           TPS++SHIQAAVICSKS GLQIR RSGGHDFEGLSYVAY PFIVVDLINLRS+ V+V+  
Sbjct: 86  TPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYRPFIVVDLINLRSVKVEVENN 145

Query: 124 TAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVI 183
           TAWV+S ATLGELYYRI EKS TL FP G CPTV  GG+ SGGGYGL+LRK+GLAADNVI
Sbjct: 146 TAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVGIGGHFSGGGYGLMLRKFGLAADNVI 205

Query: 184 DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE 243
           DAYLVDANG+  D+ESMGEDLFWAIRGGGGGSFGIVVAWK+KLVPVP TVT CS+ R  E
Sbjct: 206 DAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFGIVVAWKIKLVPVPETVTICSTDRNLE 265

Query: 244 EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKI 303
           EDAI LIH+WQYVG KL++NI  G++  GG  +++  G   P A F++ FLG+A++ V  
Sbjct: 266 EDAIKLIHRWQYVGNKLDENIYLGIILTGGNASTQA-GITNPTARFFSLFLGRADELVTT 325

Query: 304 LKEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITSKKVKIKSD 363
           L   FP+LGL K++C EASWVES +I        E +E LLNR+      T    KIKSD
Sbjct: 326 LSTIFPELGLIKQDCVEASWVESTLIIPIGVQPIETLEPLLNRTL----TTLDSTKIKSD 385

Query: 364 YVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIAY 423
           Y+ EP+P  AI+ IW R+  +QDI    ++FVPYGGRMS+ISESE  FSHRA  LFKI Y
Sbjct: 386 YITEPIPVAAIKDIWQRL-KTQDIETSQLVFVPYGGRMSQISESETPFSHRARYLFKIGY 445

Query: 424 LTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTNH 483
           + GW+D S+  + RH++W+RE+Y YMAPFVSKSPR+AY NYRDLDIGSN +KYG   T++
Sbjct: 446 VVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYTNYRDLDIGSN-NKYGK--TSY 505

Query: 484 DQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIP 522
            +AS WGLKY+GNNF+RLV +KTKVDPY+FFRHEQSIP
Sbjct: 506 KRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSIP 534

BLAST of CsaV3_1G037510.1 vs. TrEMBL
Match: tr|A0A1S3BDB7|A0A1S3BDB7_CUCME (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488652 PE=3 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 2.0e-190
Identity = 337/515 (65.44%), Postives = 398/515 (77.28%), Query Frame = 0

Query: 8   KHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILIITPSN 67
           +H+ FL CL  HS  S  I+K++YT  NSSYSSVLN SIRN RFS P TPKP+LIITPS+
Sbjct: 32  EHQNFLHCLPEHSSKSYPISKVVYTPINSSYSSVLNFSIRNFRFSKPETPKPLLIITPSH 91

Query: 68  ISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRRTAWV 127
           +SHIQAAVICSKS GLQIR RSGGHD+EGLSYVAY PFI+VDLIN+RSI+VD+   TAWV
Sbjct: 92  VSHIQAAVICSKSHGLQIRTRSGGHDYEGLSYVAYRPFIIVDLINIRSISVDIVNNTAWV 151

Query: 128 QSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVIDAYL 187
           +S ATLGELYYRI EKS TL FP G CPTV  GG+ SGGGYG LLRKYGLAADNVIDAYL
Sbjct: 152 ESGATLGELYYRIGEKSRTLAFPAGICPTVGVGGHFSGGGYGFLLRKYGLAADNVIDAYL 211

Query: 188 VDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFEEDAI 247
           VDANG  HDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPA VT CS+ +  E DA+
Sbjct: 212 VDANGMAHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPALVTICSTIKNLEGDAV 271

Query: 248 NLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKILKEK 307
            +IHQWQYV  KL ++I  G++  GG  TS   G  KP A FY+ FLGK ++ V  L   
Sbjct: 272 KVIHQWQYVANKLHEDIFLGIVLTGGN-TSTQGGIKKPTATFYSLFLGKVDELVATLSTT 331

Query: 308 FPQLGLKKEECKEASWVES-VVIAANDFTVGEPVEALLNRSALIPPITSKKVKIKSDYVK 367
           FP+ GL K++C E  W+ES ++IA+  F   E ++ LLNR+    P T    KIKSDY+K
Sbjct: 332 FPEFGLIKKDCLEVRWIESTLIIASGVFQTVESLKPLLNRT----PSTIDSTKIKSDYIK 391

Query: 368 EPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIAYLTG 427
           +P+PK AIEGIW R+  + DI    ++FVPYG RMS+ISESE  FSHRAG L+KI Y+ G
Sbjct: 392 KPIPKAAIEGIWQRL-KAGDIEAPQLVFVPYGARMSQISESETPFSHRAGYLYKIGYMVG 451

Query: 428 WEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTNHDQA 487
           W+  ++  + RH++W RE+Y YM PFVSKSPR+AYVNYRDLDIGSN +KYG   T++ QA
Sbjct: 452 WKGQNLKAKNRHISWTRELYEYMTPFVSKSPRTAYVNYRDLDIGSN-NKYGK--TSYKQA 511

Query: 488 SSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIP 522
           S WGLKY+GNNF RLV +K KVDP++FFRHEQSIP
Sbjct: 512 SIWGLKYFGNNFKRLVYVKNKVDPHDFFRHEQSIP 537

BLAST of CsaV3_1G037510.1 vs. TrEMBL
Match: tr|A0A1S3BDZ5|A0A1S3BDZ5_CUCME (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488631 PE=3 SV=1)

HSP 1 Score: 673.3 bits (1736), Expect = 4.4e-190
Identity = 332/518 (64.09%), Postives = 398/518 (76.83%), Query Frame = 0

Query: 4   ASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILII 63
           AS  KH+ FLQCL  HS     I+K+++T  NSSYSSVL+ SIRN  FS P TPKP+LII
Sbjct: 26  ASPHKHQNFLQCLSEHSSKRYPISKVVHTPINSSYSSVLDFSIRNLLFSKPETPKPLLII 85

Query: 64  TPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRR 123
           TPS++SHIQAAVICSKS GLQIR RSGGHDFEGLSYVAY PFI+VDLINLRSI +DV+  
Sbjct: 86  TPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYHPFIIVDLINLRSIKIDVETN 145

Query: 124 TAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVI 183
           TAWV+S ATLGELYYRI EKS TL FPGG CPTV  GG+ SGGGYGL+LRK+GLAADNVI
Sbjct: 146 TAWVESGATLGELYYRIGEKSRTLAFPGGVCPTVGVGGHFSGGGYGLMLRKFGLAADNVI 205

Query: 184 DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE 243
           DAYLVDANG+ HDR+SMGEDLFWAIRGGGGGSFGIVVAWK+KLV VPATVT CS++R  +
Sbjct: 206 DAYLVDANGKVHDRKSMGEDLFWAIRGGGGGSFGIVVAWKIKLVSVPATVTICSTNRNLK 265

Query: 244 EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKI 303
            D I L+HQWQYV  KL++N+  G++  GG +T+  +G   P+A F++ FLGK +  V  
Sbjct: 266 GDGIKLVHQWQYVANKLDENLFLGIMLTGGNITTTQEGIANPIATFFSLFLGKVDILVAT 325

Query: 304 LKEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITSKKVKIKSD 363
           L   FP+LG  KE+C E SW+ES +IAA      E +E LLNR+    P   +  K+KSD
Sbjct: 326 LSAIFPELGFTKEDCVETSWIESTLIAATGVGTVESLEPLLNRT----PSNLENGKMKSD 385

Query: 364 YVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIAY 423
           Y KEP+    IEG+W R+  ++DI    ++F+PYGGRMS+ISE E  FSHRAG L+KI Y
Sbjct: 386 YFKEPISIATIEGLWQRL-KAKDIETSQLIFIPYGGRMSQISEMETPFSHRAGILYKIGY 445

Query: 424 LTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTNH 483
              W++ S+  + +H++WIR+IY YM PFVSKSPRSAY NYRDLDIG+N  KYG   T+ 
Sbjct: 446 FVAWKEQSLKAKKKHISWIRDIYKYMTPFVSKSPRSAYANYRDLDIGANK-KYGK--TSV 505

Query: 484 DQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIP 522
            QAS WGLKY+GNNF RLV +KTKVDPY+FFRHEQSIP
Sbjct: 506 RQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIP 535