CsaV3_1G013250.1 (mRNA) Cucumber (Chinese Long) v3

NameCsaV3_1G013250.1
TypemRNA
OrganismCucumis sativus (Cucumber (Chinese Long) v3)
Descriptionprotein NUCLEAR FUSION DEFECTIVE 4
Locationchr1 : 8547255 .. 8549045 (-)
Sequence length1791
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGATAAATTTCGCCCTTTCCTCAAACAACTTGCAACAGGACGATGGTTTTCAGTTTTTGCTTCCTTCCTAATCATGATCGGCGCGGGTTCCACGTACGTTTTCGGTACATACTCAAAAGCAATCAAAACTCAATTCGATTACAGCCAAACCGAAATCAACACATTAGGATTCGCCAAAGATCTTGGTTCCAATCTCGGAGTTTTTGCTGGCCTTCTCGGCGAAATAGCTCCACCGTGGGTTCTGTTTGTCGTCGGTTCATTTCTCAATTTCTTTAGCTATTTCATGATTTGGCTCTCCCTTACTCACCGTATCGCTAAACCACAGCTATGGCAAATGTTCATCTATATTTGCTTAGCTGCTAATTCACAAAACTTCGCTAACACCGCCGTTTTAGTAACGAGTGTAAGAAACTTCCCTGATCGACGAGGTATAATTCTTGGACTTCTTAAAGGATTCGTCGGAATTGGAGGTGCCATTGTAACTCAATTCTATTTAGCTCTTCATGGCCATGACAATCCAGCTAGTTTGGTGCTTCTTCTGGCATGGTTTCCCACTTTAATTTCTTCTTTATTTTTCCTTTCCATCCGAACAATCAATATGCGTAGACATCCCGAAGAACTCCGAGTTCTTTACCATTTGCTTTATGTCTCAATCATTCTTGCTTTGTTTCTCTTATTTCTTACCGTTTCACAAAAACAAGCTGCATTTTCTTCAGCTGGGTATGCTAGTGGAGCTGCTGTAATTATTGGGTTACTTCTTATGCCTCTATTAATAGCCGTTAGAGAAGAACTCATGCTCTTCAAGCTCAATGGACAAACAGACAAAAACTCATCTCCCGCTGTTTTCACTCCGGAAATGAAAACTTCATCTAGTTCTACAACTAAAAACAATGAATCCTTATCCCCAATTGAAGAAATTCCCGAATTGAATTCACCAACATGTTGCTCAAACATAGTCAACAAGCCAGAACGTGGAGAAGATTTCAGTATCTTACAAGCTCTGTTTAGTAAAGACATGGGGCTTATTTTCGTTGCAACACTTTGTGGGTGTGGTTCATCAATTGCTGCCATTGATAACATCGGCCAAATCGGCGAATCGCTTGGATATCCATCTAAATCCATAAGTATCTTTGTTTCCTGGGTTTCAATATTCAGCTTCTTCGGCAGAGTAGGCTCTGGTTTCATCTCTGAAACACTTATGACAAAATACAAATTACCTCGTCCTTTAATGTTTGCTTTCTCCCATCTTCTCACTTGTATCGGTATGTTGTTCGTCGCCTTCCCGTATCCCGGCTCCATCTATGTAGCTTCGTTGACAATCGGGTTTGGATTCGGAGCGCAAGTACCGATAATTTTTGCAATACTTTCAGAGCTTTTTGGGTTGAAATATTATGCAACAATCTTCAATTGTGCTCAATTAGCAGTCCCAATCGGATCATATGTACTAAATGTGGATGTCATTGGGAAATTGTATGATATTGAAGCAACAAAAGATGGTGGCATTAGGGATGGGAATGGATTGACTTGCAAGGGAGCTCATTGTTTTAGTGGATCATTCTTGGTTTTGGCTGTGGTGGTGTTGATTGGAGGTTTGGCCTCACTTGTGTTGGCATTTAGAACAAGAAATTTTTACAAAGGAGATGTTTATAAGAAGTATAGAGAAGATATGTGGATTCCTCAATCAGATATGGAATTTTATTGTTTGGATGACAAGAAAAAGGTTGTTAACGATAATTTCCCAAGGATTGTTATGCCCCCTAAGTATAATTTTCTCAATGTAATATAG

mRNA sequence

ATGGCCGATAAATTTCGCCCTTTCCTCAAACAACTTGCAACAGGACGATGGTTTTCAGTTTTTGCTTCCTTCCTAATCATGATCGGCGCGGGTTCCACGTACGTTTTCGGTACATACTCAAAAGCAATCAAAACTCAATTCGATTACAGCCAAACCGAAATCAACACATTAGGATTCGCCAAAGATCTTGGTTCCAATCTCGGAGTTTTTGCTGGCCTTCTCGGCGAAATAGCTCCACCGTGGGTTCTGTTTGTCGTCGGTTCATTTCTCAATTTCTTTAGCTATTTCATGATTTGGCTCTCCCTTACTCACCGTATCGCTAAACCACAGCTATGGCAAATGTTCATCTATATTTGCTTAGCTGCTAATTCACAAAACTTCGCTAACACCGCCGTTTTAGTAACGAGTGTAAGAAACTTCCCTGATCGACGAGGTATAATTCTTGGACTTCTTAAAGGATTCGTCGGAATTGGAGGTGCCATTGTAACTCAATTCTATTTAGCTCTTCATGGCCATGACAATCCAGCTAGTTTGGTGCTTCTTCTGGCATGGTTTCCCACTTTAATTTCTTCTTTATTTTTCCTTTCCATCCGAACAATCAATATGCGTAGACATCCCGAAGAACTCCGAGTTCTTTACCATTTGCTTTATGTCTCAATCATTCTTGCTTTGTTTCTCTTATTTCTTACCGTTTCACAAAAACAAGCTGCATTTTCTTCAGCTGGGTATGCTAGTGGAGCTGCTGTAATTATTGGGTTACTTCTTATGCCTCTATTAATAGCCGTTAGAGAAGAACTCATGCTCTTCAAGCTCAATGGACAAACAGACAAAAACTCATCTCCCGCTGTTTTCACTCCGGAAATGAAAACTTCATCTAGTTCTACAACTAAAAACAATGAATCCTTATCCCCAATTGAAGAAATTCCCGAATTGAATTCACCAACATGTTGCTCAAACATAGTCAACAAGCCAGAACGTGGAGAAGATTTCAGTATCTTACAAGCTCTGTTTAGTAAAGACATGGGGCTTATTTTCGTTGCAACACTTTGTGGGTGTGGTTCATCAATTGCTGCCATTGATAACATCGGCCAAATCGGCGAATCGCTTGGATATCCATCTAAATCCATAAGTATCTTTGTTTCCTGGGTTTCAATATTCAGCTTCTTCGGCAGAGTAGGCTCTGGTTTCATCTCTGAAACACTTATGACAAAATACAAATTACCTCGTCCTTTAATGTTTGCTTTCTCCCATCTTCTCACTTGTATCGGTATGTTGTTCGTCGCCTTCCCGTATCCCGGCTCCATCTATGTAGCTTCGTTGACAATCGGGTTTGGATTCGGAGCGCAAGTACCGATAATTTTTGCAATACTTTCAGAGCTTTTTGGGTTGAAATATTATGCAACAATCTTCAATTGTGCTCAATTAGCAGTCCCAATCGGATCATATGTACTAAATGTGGATGTCATTGGGAAATTGTATGATATTGAAGCAACAAAAGATGGTGGCATTAGGGATGGGAATGGATTGACTTGCAAGGGAGCTCATTGTTTTAGTGGATCATTCTTGGTTTTGGCTGTGGTGGTGTTGATTGGAGGTTTGGCCTCACTTGTGTTGGCATTTAGAACAAGAAATTTTTACAAAGGAGATGTTTATAAGAAGTATAGAGAAGATATGTGGATTCCTCAATCAGATATGGAATTTTATTGTTTGGATGACAAGAAAAAGGTTGTTAACGATAATTTCCCAAGGATTGTTATGCCCCCTAAGTATAATTTTCTCAATGTAATATAG

Coding sequence (CDS)

ATGGCCGATAAATTTCGCCCTTTCCTCAAACAACTTGCAACAGGACGATGGTTTTCAGTTTTTGCTTCCTTCCTAATCATGATCGGCGCGGGTTCCACGTACGTTTTCGGTACATACTCAAAAGCAATCAAAACTCAATTCGATTACAGCCAAACCGAAATCAACACATTAGGATTCGCCAAAGATCTTGGTTCCAATCTCGGAGTTTTTGCTGGCCTTCTCGGCGAAATAGCTCCACCGTGGGTTCTGTTTGTCGTCGGTTCATTTCTCAATTTCTTTAGCTATTTCATGATTTGGCTCTCCCTTACTCACCGTATCGCTAAACCACAGCTATGGCAAATGTTCATCTATATTTGCTTAGCTGCTAATTCACAAAACTTCGCTAACACCGCCGTTTTAGTAACGAGTGTAAGAAACTTCCCTGATCGACGAGGTATAATTCTTGGACTTCTTAAAGGATTCGTCGGAATTGGAGGTGCCATTGTAACTCAATTCTATTTAGCTCTTCATGGCCATGACAATCCAGCTAGTTTGGTGCTTCTTCTGGCATGGTTTCCCACTTTAATTTCTTCTTTATTTTTCCTTTCCATCCGAACAATCAATATGCGTAGACATCCCGAAGAACTCCGAGTTCTTTACCATTTGCTTTATGTCTCAATCATTCTTGCTTTGTTTCTCTTATTTCTTACCGTTTCACAAAAACAAGCTGCATTTTCTTCAGCTGGGTATGCTAGTGGAGCTGCTGTAATTATTGGGTTACTTCTTATGCCTCTATTAATAGCCGTTAGAGAAGAACTCATGCTCTTCAAGCTCAATGGACAAACAGACAAAAACTCATCTCCCGCTGTTTTCACTCCGGAAATGAAAACTTCATCTAGTTCTACAACTAAAAACAATGAATCCTTATCCCCAATTGAAGAAATTCCCGAATTGAATTCACCAACATGTTGCTCAAACATAGTCAACAAGCCAGAACGTGGAGAAGATTTCAGTATCTTACAAGCTCTGTTTAGTAAAGACATGGGGCTTATTTTCGTTGCAACACTTTGTGGGTGTGGTTCATCAATTGCTGCCATTGATAACATCGGCCAAATCGGCGAATCGCTTGGATATCCATCTAAATCCATAAGTATCTTTGTTTCCTGGGTTTCAATATTCAGCTTCTTCGGCAGAGTAGGCTCTGGTTTCATCTCTGAAACACTTATGACAAAATACAAATTACCTCGTCCTTTAATGTTTGCTTTCTCCCATCTTCTCACTTGTATCGGTATGTTGTTCGTCGCCTTCCCGTATCCCGGCTCCATCTATGTAGCTTCGTTGACAATCGGGTTTGGATTCGGAGCGCAAGTACCGATAATTTTTGCAATACTTTCAGAGCTTTTTGGGTTGAAATATTATGCAACAATCTTCAATTGTGCTCAATTAGCAGTCCCAATCGGATCATATGTACTAAATGTGGATGTCATTGGGAAATTGTATGATATTGAAGCAACAAAAGATGGTGGCATTAGGGATGGGAATGGATTGACTTGCAAGGGAGCTCATTGTTTTAGTGGATCATTCTTGGTTTTGGCTGTGGTGGTGTTGATTGGAGGTTTGGCCTCACTTGTGTTGGCATTTAGAACAAGAAATTTTTACAAAGGAGATGTTTATAAGAAGTATAGAGAAGATATGTGGATTCCTCAATCAGATATGGAATTTTATTGTTTGGATGACAAGAAAAAGGTTGTTAACGATAATTTCCCAAGGATTGTTATGCCCCCTAAGTATAATTTTCTCAATGTAATATAG

Protein sequence

MADKFRPFLKQLATGRWFSVFASFLIMIGAGSTYVFGTYSKAIKTQFDYSQTEINTLGFAKDLGSNLGVFAGLLGEIAPPWVLFVVGSFLNFFSYFMIWLSLTHRIAKPQLWQMFIYICLAANSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAIVTQFYLALHGHDNPASLVLLLAWFPTLISSLFFLSIRTINMRRHPEELRVLYHLLYVSIILALFLLFLTVSQKQAAFSSAGYASGAAVIIGLLLMPLLIAVREELMLFKLNGQTDKNSSPAVFTPEMKTSSSSTTKNNESLSPIEEIPELNSPTCCSNIVNKPERGEDFSILQALFSKDMGLIFVATLCGCGSSIAAIDNIGQIGESLGYPSKSISIFVSWVSIFSFFGRVGSGFISETLMTKYKLPRPLMFAFSHLLTCIGMLFVAFPYPGSIYVASLTIGFGFGAQVPIIFAILSELFGLKYYATIFNCAQLAVPIGSYVLNVDVIGKLYDIEATKDGGIRDGNGLTCKGAHCFSGSFLVLAVVVLIGGLASLVLAFRTRNFYKGDVYKKYREDMWIPQSDMEFYCLDDKKKVVNDNFPRIVMPPKYNFLNVI
BLAST of CsaV3_1G013250.1 vs. NCBI nr
Match: XP_004147309.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis sativus])

HSP 1 Score: 1163.3 bits (3008), Expect = 0.0e+00
Identity = 596/596 (100.00%), Postives = 596/596 (100.00%), Query Frame = 0

Query: 1   MADKFRPFLKQLATGRWFSVFASFLIMIGAGSTYVFGTYSKAIKTQFDYSQTEINTLGFA 60
           MADKFRPFLKQLATGRWFSVFASFLIMIGAGSTYVFGTYSKAIKTQFDYSQTEINTLGFA
Sbjct: 1   MADKFRPFLKQLATGRWFSVFASFLIMIGAGSTYVFGTYSKAIKTQFDYSQTEINTLGFA 60

Query: 61  KDLGSNLGVFAGLLGEIAPPWVLFVVGSFLNFFSYFMIWLSLTHRIAKPQLWQMFIYICL 120
           KDLGSNLGVFAGLLGEIAPPWVLFVVGSFLNFFSYFMIWLSLTHRIAKPQLWQMFIYICL
Sbjct: 61  KDLGSNLGVFAGLLGEIAPPWVLFVVGSFLNFFSYFMIWLSLTHRIAKPQLWQMFIYICL 120

Query: 121 AANSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAIVTQFYLALHGHDNPASLVL 180
           AANSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAIVTQFYLALHGHDNPASLVL
Sbjct: 121 AANSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAIVTQFYLALHGHDNPASLVL 180

Query: 181 LLAWFPTLISSLFFLSIRTINMRRHPEELRVLYHLLYVSIILALFLLFLTVSQKQAAFSS 240
           LLAWFPTLISSLFFLSIRTINMRRHPEELRVLYHLLYVSIILALFLLFLTVSQKQAAFSS
Sbjct: 181 LLAWFPTLISSLFFLSIRTINMRRHPEELRVLYHLLYVSIILALFLLFLTVSQKQAAFSS 240

Query: 241 AGYASGAAVIIGLLLMPLLIAVREELMLFKLNGQTDKNSSPAVFTPEMKTSSSSTTKNNE 300
           AGYASGAAVIIGLLLMPLLIAVREELMLFKLNGQTDKNSSPAVFTPEMKTSSSSTTKNNE
Sbjct: 241 AGYASGAAVIIGLLLMPLLIAVREELMLFKLNGQTDKNSSPAVFTPEMKTSSSSTTKNNE 300

Query: 301 SLSPIEEIPELNSPTCCSNIVNKPERGEDFSILQALFSKDMGLIFVATLCGCGSSIAAID 360
           SLSPIEEIPELNSPTCCSNIVNKPERGEDFSILQALFSKDMGLIFVATLCGCGSSIAAID
Sbjct: 301 SLSPIEEIPELNSPTCCSNIVNKPERGEDFSILQALFSKDMGLIFVATLCGCGSSIAAID 360

Query: 361 NIGQIGESLGYPSKSISIFVSWVSIFSFFGRVGSGFISETLMTKYKLPRPLMFAFSHLLT 420
           NIGQIGESLGYPSKSISIFVSWVSIFSFFGRVGSGFISETLMTKYKLPRPLMFAFSHLLT
Sbjct: 361 NIGQIGESLGYPSKSISIFVSWVSIFSFFGRVGSGFISETLMTKYKLPRPLMFAFSHLLT 420

Query: 421 CIGMLFVAFPYPGSIYVASLTIGFGFGAQVPIIFAILSELFGLKYYATIFNCAQLAVPIG 480
           CIGMLFVAFPYPGSIYVASLTIGFGFGAQVPIIFAILSELFGLKYYATIFNCAQLAVPIG
Sbjct: 421 CIGMLFVAFPYPGSIYVASLTIGFGFGAQVPIIFAILSELFGLKYYATIFNCAQLAVPIG 480

Query: 481 SYVLNVDVIGKLYDIEATKDGGIRDGNGLTCKGAHCFSGSFLVLAVVVLIGGLASLVLAF 540
           SYVLNVDVIGKLYDIEATKDGGIRDGNGLTCKGAHCFSGSFLVLAVVVLIGGLASLVLAF
Sbjct: 481 SYVLNVDVIGKLYDIEATKDGGIRDGNGLTCKGAHCFSGSFLVLAVVVLIGGLASLVLAF 540

Query: 541 RTRNFYKGDVYKKYREDMWIPQSDMEFYCLDDKKKVVNDNFPRIVMPPKYNFLNVI 597
           RTRNFYKGDVYKKYREDMWIPQSDMEFYCLDDKKKVVNDNFPRIVMPPKYNFLNVI
Sbjct: 541 RTRNFYKGDVYKKYREDMWIPQSDMEFYCLDDKKKVVNDNFPRIVMPPKYNFLNVI 596

BLAST of CsaV3_1G013250.1 vs. NCBI nr
Match: XP_008464767.1 (PREDICTED: uncharacterized protein LOC103502570 [Cucumis melo])

HSP 1 Score: 1029.2 bits (2660), Expect = 5.4e-297
Identity = 519/594 (87.37%), Postives = 555/594 (93.43%), Query Frame = 0

Query: 3   DKFRPFLKQLATGRWFSVFASFLIMIGAGSTYVFGTYSKAIKTQFDYSQTEINTLGFAKD 62
           + F PF+KQL  GRWFSVFASFLIMIGAGSTYVFGTYSK IKTQF+YSQTEINTLGFAKD
Sbjct: 4   NNFLPFVKQLTAGRWFSVFASFLIMIGAGSTYVFGTYSKVIKTQFNYSQTEINTLGFAKD 63

Query: 63  LGSNLGVFAGLLGEIAPPWVLFVVGSFLNFFSYFMIWLSLTHRIAKPQLWQMFIYICLAA 122
           LGSNLGVFAG LGEIAPPWVL VVG+FLNF+SYFMIWLS+T RIAKPQLWQMF YICLAA
Sbjct: 64  LGSNLGVFAGFLGEIAPPWVLLVVGAFLNFYSYFMIWLSVTRRIAKPQLWQMFFYICLAA 123

Query: 123 NSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAIVTQFYLALHGHDNPASLVLLL 182
           NSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAI+TQF+LAL+GH+NP++LVLLL
Sbjct: 124 NSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQFFLALYGHENPSNLVLLL 183

Query: 183 AWFPTLISSLFFLSIRTINMRRHPEELRVLYHLLYVSIILALFLLFLTVSQKQAAFSSAG 242
           +WFPTLIS  FFLSIRTIN+RRHPEELRVLYHLLYVSIILALFLLFLT++QKQAAFSSAG
Sbjct: 184 SWFPTLISLAFFLSIRTINIRRHPEELRVLYHLLYVSIILALFLLFLTITQKQAAFSSAG 243

Query: 243 YASGAAVIIGLLLMPLLIAVREELMLFKLNGQTDKNSSPAVFTPEMKTSSSSTTKNNESL 302
           Y SGAAVI+GLL +PLLIAVREELMLFKL GQTD N SP VF PEMK+SS+ST KNNESL
Sbjct: 244 YTSGAAVIVGLLSIPLLIAVREELMLFKLKGQTDNNPSPPVFIPEMKSSSNSTPKNNESL 303

Query: 303 SPIEEIPELNSPTCCSNIVNKPERGEDFSILQALFSKDMGLIFVATLCGCGSSIAAIDNI 362
           +PIEEIPE NSPTC SN+ NKPERGEDF+ILQALFSKDMGLI V TL GCGSSIAAIDNI
Sbjct: 304 TPIEEIPETNSPTCLSNVFNKPERGEDFTILQALFSKDMGLILVGTLFGCGSSIAAIDNI 363

Query: 363 GQIGESLGYPSKSISIFVSWVSIFSFFGRVGSGFISETLMTKYKLPRPLMFAFSHLLTCI 422
           GQIGESLGY SKSISIFVSWVSIF+FFGRVGSGFISETLMTKYKLPRPLMFA +H  TCI
Sbjct: 364 GQIGESLGYTSKSISIFVSWVSIFNFFGRVGSGFISETLMTKYKLPRPLMFACAHFFTCI 423

Query: 423 GMLFVAFPYPGSIYVASLTIGFGFGAQVPIIFAILSELFGLKYYATIFNCAQLAVPIGSY 482
           GMLFVAFPYPGSIY ASL IGFGFGAQVP++FAILSELFGLKYYATIFNCAQLAVPIGSY
Sbjct: 424 GMLFVAFPYPGSIYGASLIIGFGFGAQVPMLFAILSELFGLKYYATIFNCAQLAVPIGSY 483

Query: 483 VLNVDVIGKLYDIEATKDGGIRDGNGLTCKGAHCFSGSFLVLAVVVLIGGLASLVLAFRT 542
           +LNVDVIGK YD EATK G  RDG GLTCKG HCFSGSFLVL+VVVLIGG+ASLVLAFRT
Sbjct: 484 ILNVDVIGKFYDHEATKGGSTRDGKGLTCKGTHCFSGSFLVLSVVVLIGGVASLVLAFRT 543

Query: 543 RNFYKGDVYKKYREDMWIPQSDMEFYCLDDKKKVVNDNFPRIVMPPKYNFLNVI 597
           R+FYKGDVYKKYREDMWIPQSDMEFYCLD+KKKVVN+N PR+VMPPKY+FLN+I
Sbjct: 544 RDFYKGDVYKKYREDMWIPQSDMEFYCLDNKKKVVNNNLPRVVMPPKYSFLNII 597

BLAST of CsaV3_1G013250.1 vs. NCBI nr
Match: XP_023521059.1 (uncharacterized protein LOC111784657 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 888.6 bits (2295), Expect = 1.1e-254
Identity = 450/600 (75.00%), Postives = 517/600 (86.17%), Query Frame = 0

Query: 1   MADKFRP----FLKQLATGRWFSVFASFLIMIGAGSTYVFGTYSKAIKTQFDYSQTEINT 60
           MAD   P    F+KQ+  GRWF+VFASFLIMIGAGSTY+FGTYSKAIK+QFDY+QT+INT
Sbjct: 1   MADNSLPESWRFIKQVVEGRWFAVFASFLIMIGAGSTYIFGTYSKAIKSQFDYNQTQINT 60

Query: 61  LGFAKDLGSNLGVFAGLLGEIAPPWVLFVVGSFLNFFSYFMIWLSLTHRIAKPQLWQMFI 120
           LGFAKDLGSN GVFAGLLGE+APPWVLF+VGS LNF+SYFMIWLS+T RIAKP  W+MF+
Sbjct: 61  LGFAKDLGSNFGVFAGLLGEVAPPWVLFLVGSALNFWSYFMIWLSITGRIAKPPFWEMFL 120

Query: 121 YICLAANSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAIVTQFYLALHGHDNPA 180
           +IC+AANSQNFANTA+LVTSVRNFPDRRGIILGLLKGFVG GGAI+TQFYLAL+GHDNP 
Sbjct: 121 FICVAANSQNFANTAILVTSVRNFPDRRGIILGLLKGFVGFGGAILTQFYLALYGHDNPT 180

Query: 181 SLVLLLAWFPTLISSLFFLSIRTINMRRHPEELRVLYHLLYVSIILALFLLFLTVSQKQA 240
           +LVLLL+W PT++S LFFLSIR I +R+HP+ELRVLYHLLYVSIILALFLLFLT++QKQ 
Sbjct: 181 NLVLLLSWLPTVVSMLFFLSIRPIKVRKHPDELRVLYHLLYVSIILALFLLFLTITQKQT 240

Query: 241 AFSSAGYASGAAVIIGLLLMPLLIAVREELMLFKLNGQT-DKNSSPAVFTPEMKTSSSST 300
            F+   Y SGA VIIGLLL+PLLIA+REE MLFKLN QT + N +  +  PE + S  + 
Sbjct: 241 VFTQEAYMSGATVIIGLLLIPLLIAIREEFMLFKLNKQTLNNNPTVPILMPEHEPSPKAP 300

Query: 301 TKNNESLSPIEEIPELNSPTCCSNIVNKPERGEDFSILQALFSKDMGLIFVATLCGCGSS 360
                    I++IPE  S +C S    KP+RGEDF+ILQALFSKDM L+F+ATL  CG+S
Sbjct: 301 -------PTIQQIPEA-STSCFSMAFKKPQRGEDFTILQALFSKDMALVFIATLTACGAS 360

Query: 361 IAAIDNIGQIGESLGYPSKSISIFVSWVSIFSFFGRVGSGFISETLMTKYKLPRPLMFAF 420
           IAAIDN+GQ+GESLGYPS+SISIFVSWVSIF+FFGRV SGFISE LMTKYKLPRPLMFAF
Sbjct: 361 IAAIDNLGQVGESLGYPSQSISIFVSWVSIFNFFGRVFSGFISEALMTKYKLPRPLMFAF 420

Query: 421 SHLLTCIGMLFVAFPYPGSIYVASLTIGFGFGAQVPIIFAILSELFGLKYYATIFNCAQL 480
           SHLLTCIG+LF+AFPY GS+Y ASL IGFGFGAQVP++FAI+SELFGLKYY+TIFNC QL
Sbjct: 421 SHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPMVFAIISELFGLKYYSTIFNCGQL 480

Query: 481 AVPIGSYVLNVDVIGKLYDIEATKDGGIRDGNGLTCKGAHCFSGSFLVLAVVVLIGGLAS 540
           AVPIGSY+LNVDVIGKLYDIEA K GG+++G GLTC G  CFSGSFL+LAVVVL G + S
Sbjct: 481 AVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTGTPCFSGSFLILAVVVLFGAVVS 540

Query: 541 LVLAFRTRNFYKGDVYKKYREDMWIPQSDMEFYCLDDKKKVVNDNFPRIVMPPKYNFLNV 596
           LVLA+RT++FYKGDVY KYREDMWIPQSDMEFYC+DD+KK  N N PR+ MPPKYNFL +
Sbjct: 541 LVLAYRTQDFYKGDVYNKYREDMWIPQSDMEFYCIDDRKKDGN-NLPRMDMPPKYNFLKL 591

BLAST of CsaV3_1G013250.1 vs. NCBI nr
Match: XP_022964772.1 (uncharacterized protein LOC111464772 [Cucurbita moschata])

HSP 1 Score: 887.1 bits (2291), Expect = 3.3e-254
Identity = 449/600 (74.83%), Postives = 517/600 (86.17%), Query Frame = 0

Query: 1   MADKFRP----FLKQLATGRWFSVFASFLIMIGAGSTYVFGTYSKAIKTQFDYSQTEINT 60
           MAD   P    F+KQ+  GRWF+VFASFLIMIGAGSTY+FGTYSKAIK+QFDY+QT+INT
Sbjct: 1   MADNSLPESWRFIKQVVEGRWFAVFASFLIMIGAGSTYIFGTYSKAIKSQFDYNQTQINT 60

Query: 61  LGFAKDLGSNLGVFAGLLGEIAPPWVLFVVGSFLNFFSYFMIWLSLTHRIAKPQLWQMFI 120
           LGFAKDLGSN GVFAGLLGE+APPWVLF+VGS LNF+SYFMIWLS+T RIAKP  W+MF+
Sbjct: 61  LGFAKDLGSNFGVFAGLLGEVAPPWVLFLVGSALNFWSYFMIWLSITGRIAKPPFWEMFL 120

Query: 121 YICLAANSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAIVTQFYLALHGHDNPA 180
           +IC+AANSQNFANTA+LVTSVRNFPDRRGIILGLLKGFVG GGAI+TQFYLAL+GHDNP 
Sbjct: 121 FICVAANSQNFANTAILVTSVRNFPDRRGIILGLLKGFVGFGGAILTQFYLALYGHDNPT 180

Query: 181 SLVLLLAWFPTLISSLFFLSIRTINMRRHPEELRVLYHLLYVSIILALFLLFLTVSQKQA 240
           +LVLLL+W PT+ S LFFLSIR I +R+ P+ELRVLYHLLYVSIILALFLLFLT++QKQ 
Sbjct: 181 NLVLLLSWLPTVASMLFFLSIRPIKVRKDPDELRVLYHLLYVSIILALFLLFLTITQKQT 240

Query: 241 AFSSAGYASGAAVIIGLLLMPLLIAVREELMLFKLNGQT-DKNSSPAVFTPEMKTSSSST 300
            F+   Y SGA VIIGLLL+PLLIA+REE MLFKLN QT + N +  +  PE + S  + 
Sbjct: 241 VFTQEAYMSGATVIIGLLLIPLLIAIREEFMLFKLNKQTLNNNPTVPILMPEHEPSPKAP 300

Query: 301 TKNNESLSPIEEIPELNSPTCCSNIVNKPERGEDFSILQALFSKDMGLIFVATLCGCGSS 360
                    I++IPE  S +C S    KP+RGEDF+ILQALFSKDM L+F+ATL  CG+S
Sbjct: 301 -------PTIQQIPEA-STSCFSMTFKKPQRGEDFTILQALFSKDMALVFIATLTACGAS 360

Query: 361 IAAIDNIGQIGESLGYPSKSISIFVSWVSIFSFFGRVGSGFISETLMTKYKLPRPLMFAF 420
           IAAIDN+GQ+GESLGYPS++ISIFVSWVSIF+FFGRV SGFISE LMTKYKLPRPLMFAF
Sbjct: 361 IAAIDNLGQVGESLGYPSQTISIFVSWVSIFNFFGRVFSGFISEALMTKYKLPRPLMFAF 420

Query: 421 SHLLTCIGMLFVAFPYPGSIYVASLTIGFGFGAQVPIIFAILSELFGLKYYATIFNCAQL 480
           SHLLTCIG+LF+AFPY GS+Y ASL IGFGFGAQVP++FAI+SELFGLKYY+TIFNC QL
Sbjct: 421 SHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPMVFAIISELFGLKYYSTIFNCGQL 480

Query: 481 AVPIGSYVLNVDVIGKLYDIEATKDGGIRDGNGLTCKGAHCFSGSFLVLAVVVLIGGLAS 540
           AVPIGSY+LNVDVIGKLYDIEA K GG+++G GLTC G HCFSGSFL+LAVVVL+G + S
Sbjct: 481 AVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTGTHCFSGSFLILAVVVLLGAVVS 540

Query: 541 LVLAFRTRNFYKGDVYKKYREDMWIPQSDMEFYCLDDKKKVVNDNFPRIVMPPKYNFLNV 596
           LVLA+RT++FYKGDVY KYREDMWIPQSDMEFYC+DD+KK  N N PR+ MPPKYNFL +
Sbjct: 541 LVLAYRTQDFYKGDVYNKYREDMWIPQSDMEFYCIDDRKKGGN-NLPRMDMPPKYNFLKL 591

BLAST of CsaV3_1G013250.1 vs. NCBI nr
Match: XP_022970238.1 (uncharacterized protein LOC111469246 [Cucurbita maxima])

HSP 1 Score: 883.2 bits (2281), Expect = 4.8e-253
Identity = 446/600 (74.33%), Postives = 517/600 (86.17%), Query Frame = 0

Query: 1   MADKFRP----FLKQLATGRWFSVFASFLIMIGAGSTYVFGTYSKAIKTQFDYSQTEINT 60
           MAD   P    F+KQ+  GRWF+VFASFLIMIGAGSTY+FGTYSKAIK+QF+Y+QT+INT
Sbjct: 1   MADNSLPESWRFIKQVVEGRWFAVFASFLIMIGAGSTYIFGTYSKAIKSQFNYNQTQINT 60

Query: 61  LGFAKDLGSNLGVFAGLLGEIAPPWVLFVVGSFLNFFSYFMIWLSLTHRIAKPQLWQMFI 120
           LGFAKDLGSN GVFAGLLGE+APPWVLF+VGS LNF+SYFMIWLS+T RIAKP  W+MF+
Sbjct: 61  LGFAKDLGSNFGVFAGLLGEVAPPWVLFLVGSALNFWSYFMIWLSITGRIAKPPFWEMFL 120

Query: 121 YICLAANSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAIVTQFYLALHGHDNPA 180
           +IC+AANSQNFANTA+LVTSVRNFPDRRGIILGLLKGFVG GGAI+TQFYLAL+GHDNP 
Sbjct: 121 FICVAANSQNFANTAILVTSVRNFPDRRGIILGLLKGFVGFGGAILTQFYLALYGHDNPT 180

Query: 181 SLVLLLAWFPTLISSLFFLSIRTINMRRHPEELRVLYHLLYVSIILALFLLFLTVSQKQA 240
           +LVLLL+W PT++S LFFLSIR I +R+HP+ELRVLYHLLYVSIILALFLLFLT++QKQ 
Sbjct: 181 NLVLLLSWLPTVVSMLFFLSIRPIKVRKHPDELRVLYHLLYVSIILALFLLFLTITQKQT 240

Query: 241 AFSSAGYASGAAVIIGLLLMPLLIAVREELMLFKLNGQT-DKNSSPAVFTPEMKTSSSST 300
            F+   Y SGA VIIGLLL+PLLIA+REE +LFKLN QT + N +  +  PE + S  + 
Sbjct: 241 VFTQEAYMSGATVIIGLLLIPLLIAIREEFLLFKLNKQTLNNNPTVPILMPEHEPSPKAP 300

Query: 301 TKNNESLSPIEEIPELNSPTCCSNIVNKPERGEDFSILQALFSKDMGLIFVATLCGCGSS 360
                    IE+IPE  S +C S    +P+RGEDF+ILQALFSKDM L+F+ATL  CG+S
Sbjct: 301 -------PTIEQIPEA-STSCFSMAFKRPQRGEDFTILQALFSKDMALVFIATLSACGAS 360

Query: 361 IAAIDNIGQIGESLGYPSKSISIFVSWVSIFSFFGRVGSGFISETLMTKYKLPRPLMFAF 420
           IAAIDN+GQ+GESLGYPS++ISIFVSWVSIF+FFGRV SGFISE LMTKYKLPRPLMFAF
Sbjct: 361 IAAIDNLGQVGESLGYPSQTISIFVSWVSIFNFFGRVFSGFISEALMTKYKLPRPLMFAF 420

Query: 421 SHLLTCIGMLFVAFPYPGSIYVASLTIGFGFGAQVPIIFAILSELFGLKYYATIFNCAQL 480
           SHLLTCIG+LF+AFPY GS+Y ASL IGFGFGAQVP++FAI+SELFGLKYY+TIFNC QL
Sbjct: 421 SHLLTCIGILFIAFPYKGSVYAASLIIGFGFGAQVPMVFAIISELFGLKYYSTIFNCGQL 480

Query: 481 AVPIGSYVLNVDVIGKLYDIEATKDGGIRDGNGLTCKGAHCFSGSFLVLAVVVLIGGLAS 540
           AVPIGSY+LNVDVIGKLYDIEA K GG+++G GLTC G  CFSGSFL+LAVVVL G + S
Sbjct: 481 AVPIGSYILNVDVIGKLYDIEAAKLGGVKNGKGLTCTGTPCFSGSFLILAVVVLFGAVVS 540

Query: 541 LVLAFRTRNFYKGDVYKKYREDMWIPQSDMEFYCLDDKKKVVNDNFPRIVMPPKYNFLNV 596
           LVLA+RT++FYKGDVY +YREDMWIPQSDMEFYC+DD+KK  N N PR+ MPPKYNFL +
Sbjct: 541 LVLAYRTQDFYKGDVYNRYREDMWIPQSDMEFYCIDDRKKGGN-NLPRMDMPPKYNFLKL 591

BLAST of CsaV3_1G013250.1 vs. TAIR10
Match: AT2G28120.1 (Major facilitator superfamily protein)

HSP 1 Score: 572.8 bits (1475), Expect = 2.5e-163
Identity = 303/578 (52.42%), Postives = 398/578 (68.86%), Query Frame = 0

Query: 8   FLKQLATGRWFSVFASFLIMIGAGSTYVFGTYSKAIKTQFDYSQTEINTLGFAKDLGSNL 67
           FL     GRWF VFASFLIM  AG+TY+FGTYSK IK+   Y QT +N LGF KDLG+N+
Sbjct: 9   FLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANV 68

Query: 68  GVFAGLLGEIAPPWVLFVVGSFLNFFSYFMIWLSLTHRIAKPQLWQMFIYICLAANSQNF 127
           GV +GL+ E+ P W +  +GS +NF  YFMIWL++T ++AKP++WQM +YIC+ ANSQNF
Sbjct: 69  GVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNF 128

Query: 128 ANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAIVTQFYLALHGHDNPASLVLLLAWFPT 187
           ANT  LVT V+NFP+ RG++LGLLKG+VG+ GAI TQ Y A++GHD+  SL+LL+AW P 
Sbjct: 129 ANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDS-KSLILLIAWLPA 188

Query: 188 LISSLFFLSIRTINMRRHPEELRVLYHLLYVSIILALFLLFLTVSQKQAAFSSAGYASGA 247
            +S +F   IR   + R   EL V Y  LY+SI LALFL+ + +++KQ  FS A YA+ A
Sbjct: 189 AVSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAASA 248

Query: 248 AVIIGLLLMPLLIAVREELMLFK-LNGQTDKNSSPAVFTP--EMKTSSSSTTKNNESLSP 307
            +   LL +PL ++V++EL ++  +    ++ S   V  P  E+        K N     
Sbjct: 249 TICCALLFVPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVNGE--- 308

Query: 308 IEEIPELNSPTCCSNIVNKPERGEDFSILQALFSKDMGLIFVATLCGCGSSIAAIDNIGQ 367
                E  + +C S + + P RGED++ILQAL S DM ++FVAT CG GSS+ A+DN+GQ
Sbjct: 309 -----EKETKSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQ 368

Query: 368 IGESLGYPSKSISIFVSWVSIFSFFGRVGSGFISETLMTKYKLPRPLMFAFSHLLTCIGM 427
           IGESLGYP+ ++S FVS VSI+++FGRV SGF+SE L+ KYKLPRPLM     LL+C G 
Sbjct: 369 IGESLGYPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGH 428

Query: 428 LFVAFPYPGSIYVASLTIGFGFGAQVPIIFAILSELFGLKYYATIFNCAQLAVPIGSYVL 487
           L +AFP PGS+Y+AS+ +GF FGAQ+P++FAI+SELFGLKYY+T+FNC QLA P+GSY+L
Sbjct: 429 LLIAFPVPGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYIL 488

Query: 488 NVDVIGKLYDIEATKDGGIR-----DGNGLTCKGAHCFSGSFLVLAVVVLIGGLASLVLA 547
           NV V G LYD EA K    R     D   LTC G+ C+   FL+LA V   G L SL LA
Sbjct: 489 NVRVTGMLYDKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLA 548

Query: 548 FRTRNFYKGDVYKKYREDMWIPQSDMEFYCLDDKKKVV 578
            RTR FYKGD+YKK+RE    P+S+ E   + D +K V
Sbjct: 549 IRTREFYKGDIYKKFRES---PESESEL--VPDSRKAV 572

BLAST of CsaV3_1G013250.1 vs. TAIR10
Match: AT2G39210.1 (Major facilitator superfamily protein)

HSP 1 Score: 515.8 bits (1327), Expect = 3.6e-146
Identity = 266/560 (47.50%), Postives = 367/560 (65.54%), Query Frame = 0

Query: 11  QLATGRWFSVFASFLIMIGAGSTYVFGTYSKAIKTQFDYSQTEINTLGFAKDLGSNLGVF 70
           Q+ TGRWF  F S LIM  AG+TY+FG YS  IK    Y QT +N L F KDLG+N+GV 
Sbjct: 16  QILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVL 75

Query: 71  AGLLGEIAPPWVLFVVGSFLNFFSYFMIWLSLTHRIAKPQLWQMFIYICLAANSQNFANT 130
           AGLL E+ PPW + ++G+ LNFF YFMIWL++T RI+KPQ+W M +YIC+ ANSQ+FANT
Sbjct: 76  AGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANT 135

Query: 131 AVLVTSVRNFPDRRGIILGLLKGFVGIGGAIVTQFYLALHGHDNPASLVLLLAWFPTLIS 190
             LVT V+NFP+ RG++LG+LKG+VG+ GAI+TQ Y A +G D    L+L++ W P ++S
Sbjct: 136 GSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDT-KELILMIGWLPAIVS 195

Query: 191 SLFFLSIRTINMRRHPEELRVLYHLLYVSIILALFLLFLTVSQKQAAFSSAGYASGAAVI 250
             F  +IR + ++R   EL+V Y+ LY+S+ LA FL+ + +  K + F+ +         
Sbjct: 196 FAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEXXXXXXXX 255

Query: 251 IGLLLMPLLIAVREELMLFKLNGQTDKNSSPAVFTPEMKTSSSSTTKNNESLSPIEEIPE 310
                             +K       + +P     E     SS  K+++     E + +
Sbjct: 256 XXXXXXXXXXXXXXXXXXWKEKQVALNDPAPINVVTEKPKLDSSEFKDDDGEESKEVVEK 315

Query: 311 LNSPTCCSNIVNKPERGEDFSILQALFSKDMGLIFVATLCGCGSSIAAIDNIGQIGESLG 370
           + +P+C + + N PERG+D++ILQALFS DM ++F+AT+CG G ++ AIDN+GQIG SLG
Sbjct: 316 VKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIGNSLG 375

Query: 371 YPSKSISIFVSWVSIFSFFGRVGSGFISETLMTKYKLPRPLMFAFSHLLTCIGMLFVAFP 430
           YP +S+S FVS VSI++++GRV SG +SE  + KYK PRPLM     LL+C G L +AF 
Sbjct: 376 YPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIAFN 435

Query: 431 YPGSIYVASLTIGFGFGAQVPIIFAILSELFGLKYYATIFNCAQLAVPIGSYVLNVDVIG 490
            PG +YVAS+ IGF FGAQ P++FAI+SE+FGLKYY+T++N   +A PIGSY+LNV V G
Sbjct: 436 VPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLNVRVAG 495

Query: 491 KLYDIEATKD----GGIR-DGNGLTCKGAHCFSGSFLVLAVVVLIGGLASLVLAFRTRNF 550
            LYD+EA K     G  R +G  L C G  CF  SF+++A V L G L S+VL  RT+ F
Sbjct: 496 YLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVIRTKKF 555

Query: 551 YKGDVYKKYREDMWIPQSDM 566
           YK D+YKK+RE     + +M
Sbjct: 556 YKSDIYKKFREKALAAEMEM 574

BLAST of CsaV3_1G013250.1 vs. TAIR10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein)

HSP 1 Score: 302.0 bits (772), Expect = 8.2e-82
Identity = 199/556 (35.79%), Postives = 309/556 (55.58%), Query Frame = 0

Query: 16  RWFSVFASFLIMIGAGSTYVFGTYSKAIKTQFDYSQTEINTLGFAKDLGSNLGVFAGLL- 75
           +W ++ AS  I   +G++Y FG YS  +K+   Y Q+ ++T+   KD+G+N GVF+GLL 
Sbjct: 7   KWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVFSGLLY 66

Query: 76  ---------------GEIAPPWVLFVVGSFLNFFSYFMIWLSLTHRIAKPQLWQMFIYIC 135
                          G    PWV+  VG+   F  YF+IW S+T  I KP +  M +++ 
Sbjct: 67  TYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLMCLFMF 126

Query: 136 LAANSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAIVTQFYLALHGHDNPASLV 195
           LAA SQ F NTA +V++V NF D  G  +G++KGF+G+ GAI+ Q Y  L   D PAS +
Sbjct: 127 LAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGD-PASFI 186

Query: 196 LLLAWFPTLISSLFFLSIRTINMRRHPEELRVLYHLLYVSIILALFLLFLTVSQKQAAFS 255
           LLLA  PT++S L    +R I      ++ + L  L  VS+I+A +L+ + + +     S
Sbjct: 187 LLLAVTPTVLSLLVMPLVR-IYETSVADDKKHLNGLSAVSLIIAAYLMIIIILKNTFGLS 246

Query: 256 SAGYASGAAVIIGLLLMPLLIAVREELMLFKLNGQTDKNSSPAVFTPEMKTSSSSTTKNN 315
           S         ++ +L +PLLIA R +     +      + SP + +P+  TS + +++ +
Sbjct: 247 SWANIVTLVCLLVMLALPLLIARRAQ--RDGMEKTVPHDYSPLISSPKATTSGNQSSEGD 306

Query: 316 ESLSPIEEIPELNSPTCCSNIVNKPERG--EDFSILQALFSKDMGLIFVATLCGCGSSIA 375
                                 +K E G  E+ ++LQA+      L+F+A +CG GS ++
Sbjct: 307 ----------------------SKVEAGLSENLNLLQAMKKLSFWLLFLAMICGMGSGLS 366

Query: 376 AIDNIGQIGESLGYPSKSISIFVSWVSIFSFFGRVGSGFISETLMTKYKLPRPLMFAFSH 435
            I+NI QIGESL Y S  I+  VS  SI++F GR G+G+ S+ L+ K   PRPL+ A + 
Sbjct: 367 TINNIRQIGESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATL 426

Query: 436 LLTCIGMLFVAFPYPGSIYVASLTIGFGFGAQVPIIFAILSELFGLKYYATIFNCAQLAV 495
               IG L +A  + G++YV S+ +G  +G+Q  ++  I SELFG+++  TIFN   +A 
Sbjct: 427 GTMSIGHLIIASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVAS 486

Query: 496 PIGSYVLNVDVIGKLYDIEATKDGGIRDGNGLTCKGAHCFSGSFLVLAVVVLIGGLASLV 554
           PIGSY+ +V +IG +YD  A+       G G TC G+HCF  SF+++A V   G L ++V
Sbjct: 487 PIGSYIFSVRLIGYIYDKTAS-------GEGNTCYGSHCFRLSFIIMASVAFFGFLVAIV 529

BLAST of CsaV3_1G013250.1 vs. TAIR10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein)

HSP 1 Score: 283.9 bits (725), Expect = 2.3e-76
Identity = 188/553 (34.00%), Postives = 302/553 (54.61%), Query Frame = 0

Query: 16  RWFSVFASFLIMIGAGSTYVFGTYSKAIKTQFDYSQTEINTLGFAKDLGSNLGVFAGLLG 75
           +W ++ AS  I   AG +Y FG YS  +K+   Y Q+ ++T+   KD+G N+GV +GL+ 
Sbjct: 9   KWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNVGVLSGLVY 68

Query: 76  EIAP--------------PWVLFVVGSFLNFFSYFMIWLSLTHRIAKPQLWQMFIYICLA 135
             A               PWV+ ++G+ LNF  YF++W S+T  I +P +  M +++ +A
Sbjct: 69  TAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPVMCLFMFIA 128

Query: 136 ANSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAIVTQFYLALHGHDNPASLVLL 195
           A S  F NTA +V+S+ NF D  G  +G++KGFVG+ GA++ Q Y  +   D P + +LL
Sbjct: 129 AQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGD-PKTFILL 188

Query: 196 LAWFPTLISSLFFLSIR-----TINMRRHPEELRVLYHLLYVSIILALFLLFLTVSQKQA 255
           LA  P+L+S L    +R     T++ ++H + L  L      S+I+A +L+   + +   
Sbjct: 189 LAIVPSLLSVLVMPLVRVYKTSTVDEKKHLDGLSTL------SLIIAAYLMITIILKSTL 248

Query: 256 AFSSAGYASGAAVIIGLLLMPLLIAVREELMLFKLNGQTDKNSSP--AVFTPEMKTSSSS 315
           +  S   A   AV++ LL  PLL+AVR            D    P  +V++P +    ++
Sbjct: 249 SLPSWANAVTLAVLLVLLSSPLLVAVR---------AHRDSIEKPLSSVYSPLVDNLEAT 308

Query: 316 TTKNNESLSPIEEIPELNSPTCCSNIVNKPERGEDFSILQALFSKDMGLIFVATLCGCGS 375
           T   +  +  ++E   LN                   +LQA+ + D  L+F+A +CG GS
Sbjct: 309 T---SGEILMLDEDKSLN-------------------LLQAMCNVDFWLLFLAMICGMGS 368

Query: 376 SIAAIDNIGQIGESLGYPSKSISIFVSWVSIFSFFGRVGSGFISETLMTKYKLPRPLMFA 435
            I+ I+NI QIGESL Y S  I+  ++  +I++F GR G G++S+ L+ +   PRPL+ A
Sbjct: 369 GISTINNIRQIGESLRYTSVEINSLLALWNIWNFIGRFGGGYVSDWLLHRKGWPRPLLMA 428

Query: 436 FSHLLTCIGMLFVAFPYPGSIYVASLTIGFGFGAQVPIIFAILSELFGLKYYATIFNCAQ 495
            +     IG L +A  + G++Y  S+ +G  +G+Q  ++  I SELFG+K+  TI+N   
Sbjct: 429 TTLGTMTIGHLIIASGFQGNLYPGSIIVGICYGSQWSLMPTITSELFGVKHMGTIYNTIS 488

Query: 496 LAVPIGSYVLNVDVIGKLYDIEATKDGGIRDGNGLTCKGAHCFSGSFLVLAVVVLIGGLA 548
           +A P+GSY+ +V +IG +YD           G G TC G HCF  +++V+A V  +G L 
Sbjct: 489 IASPMGSYIFSVRLIGYIYDRTII-------GEGNTCYGPHCFRLAYVVIASVAFLGFLV 516

BLAST of CsaV3_1G013250.1 vs. TAIR10
Match: AT5G50630.1 (Major facilitator superfamily protein)

HSP 1 Score: 283.9 bits (725), Expect = 2.3e-76
Identity = 185/555 (33.33%), Postives = 301/555 (54.23%), Query Frame = 0

Query: 1   MADKFRPFLKQLATGRWFSVFASFLIMIGAGSTYVF-GTYSKAIKTQFDYSQTEINTLGF 60
           M   +R  L+ L   RW     +  +   AG  Y+F G+ S AIKT   Y+Q +I  LG 
Sbjct: 1   MMTLWRHKLELLVNDRWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGV 60

Query: 61  AKDLGSNLGVFAGLLGEIAPPWVLFVVGSFLNFFSYFMIWLSLTHRIAKPQLWQMFIYIC 120
           AK+LG  +G  +G L E++P WV+ +VG+  N F Y ++WL +T ++    LW +F+ I 
Sbjct: 61  AKNLGDAIGFVSGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIF 120

Query: 121 LAANSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAIVTQFYLALH-GHDNPASL 180
           +  N + + NTA LV+ + NFP+ RG ++G+LKGF G+ GAI+TQ YL  +  HD  +S+
Sbjct: 121 VGTNGETYYNTASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYLMFNPSHD--SSV 180

Query: 181 VLLLAWFPTLISSLFFLSIRTI------NMRRHPEELRVLYHLLYVSIILALFLLFLTVS 240
           +L++A  P ++       +R +      N+R   ++LR L  +    ++LA++LL L V 
Sbjct: 181 ILMVALGPPVVVLALLFIVRPVERSCRTNLR--SDDLRFL-AIYGFCVVLAVYLLGLLVL 240

Query: 241 QKQAAFSSAGYASGAAVIIGLLLMPLLIAVREELMLFKLNGQTDKNSSPAVFTPEMKTSS 300
           Q     +     +  A+++  +++P+L+      +           ++     PE  TS+
Sbjct: 241 QSVFDMTQTIITTSGAILVIFMVVPVLVPFSSVFI---------SGNNVTSVKPEEGTSN 300

Query: 301 SSTTKNNESLSPIEEIPELNSPTCCSNIVNKPERGEDFSILQALFSKDMGLIFVATLCGC 360
               +    +   +  PE     C          GEDF++LQAL   D  LIF++ + G 
Sbjct: 301 VDQHEARTLIERSDRPPEKKRAPCI---------GEDFTLLQALGQADFWLIFMSLVLGV 360

Query: 361 GSSIAAIDNIGQIGESLGYPSKSISIFVSWVSIFSFFGRVGSGFISETLMTKYKLPRPLM 420
           GS I  IDN+GQI  SLGY   +  IFVS +SI +F GRV  G+ SE ++ K  LPR L 
Sbjct: 361 GSGITIIDNLGQICYSLGY--SNTKIFVSLISISNFLGRVAGGYFSELIIRKLSLPRTLA 420

Query: 421 FAFSHLLTCIGMLFVAFPYPGSIYVASLTIGFGFGAQVPIIFAILSELFGLKYYATIFNC 480
            +    +  +G+++ A  +PG IYV ++ IG G+GA   I  A +S++FGLK + +++N 
Sbjct: 421 MSVVQAIMSLGLIYYAIDWPGKIYVVTIVIGMGYGAHWAIAPASVSDIFGLKSFGSLYNF 480

Query: 481 AQLAVPIGSYVLNVDVIGKLYDIEATKDGG-IRDGNGLTCKGAHCFSGSFLVLAVVVLIG 540
              A+PIGS+V +  +   +YD  A K  G   +   L C G+ C+S +  +++++ L+ 
Sbjct: 481 QITALPIGSFVFSGVIASNIYDYYARKQAGPTTETESLVCTGSVCYSVTCSLMSMLCLMA 530

Query: 541 GLASLVLAFRTRNFY 547
            + SL + +RTR FY
Sbjct: 541 MVLSLSVVYRTRKFY 530

BLAST of CsaV3_1G013250.1 vs. Swiss-Prot
Match: sp|F4I9E1|NFD4_ARATH (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 5.5e-27
Identity = 143/546 (26.19%), Postives = 238/546 (43.59%), Query Frame = 0

Query: 16  RWFSVFASFLIMIGAGSTYVFGTYSKAIKTQFDYSQTEINTLGFAKDLGSNLGVFAGLLG 75
           +W  + A+  I    G+ + F  YS  +K+    SQ  +N L  A DLG   G  +G+  
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 76  EIAPPWVLFVVGSFLNFFSYFMIWLSLTHRIAKPQLWQMFIYICLAANSQNFANTAVLVT 135
              P  V+    + + F  Y + WL +T+ I  P    +F+   LA  S  + NTA  + 
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLPYS-LVFLCCLLAGLSICWFNTACFIL 162

Query: 136 SVRNFPDRRGIILGLLKGFVGIGGAIVTQFYLALHGHDNPA-----SLVLLLAWFPTLIS 195
            +R+FP+ R + L L   F GI  A+ +  + A++   +       SLV L+  F  L  
Sbjct: 163 CIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVVSFAALYP 222

Query: 196 SLFFLSIRT---INMRRHPEELRVLYHLLYVSIILALFLLFLTVSQKQAAFSSAGYASGA 255
            L   S+ T    + RRH      ++ +L V  ++  F L L+ S   +A     +    
Sbjct: 223 VLTKPSLDTTPDYDSRRHDSH---VFTILNVLAVITSFHLLLSSSSTSSA--RLNFIGAV 282

Query: 256 AVIIGLLLMPLLIAVREELMLFKLNGQTDKNSSPAVF-----TPEMKTSSSSTTKNNESL 315
            +++  L  PLL+  R +  L  +N + +  SS  V          KTS SS T   E +
Sbjct: 283 VLLVFPLCAPLLVYAR-DYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKT-GYEHM 342

Query: 316 SPIEEIPELNSPTCCSNIVNKPERGEDFSILQALFSKDMGLIFVATLCGCGSSIAAIDNI 375
              +E              N    G++ S    +   +  L ++A  CG    +   +N+
Sbjct: 343 GTAKE-------------GNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNL 402

Query: 376 GQIGESLGYPSKSISIFVSWVSIFSFFGRVGSGFISETLMTKYKLPRPLMFAFSHLLTCI 435
           GQI +SLG  S ++   V+  S FSFFGR+ S    + +  +++L R   FA + L T I
Sbjct: 403 GQIAQSLGQNSTTL---VTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPI 462

Query: 436 G--MLFVAFPYPGSIYVASLTIGFGFGAQVPIIFAILSELFGLKYYATIFNCAQLAVPIG 495
              +L V+     ++  A+  IG   G       +I S+LFG        N     +PIG
Sbjct: 463 AFFLLAVSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIG 522

Query: 496 SYVLNVDVIGKLYDIEATKDGGIRDGNGLTCKGAHCFSGSFLVLAVVVLIGGLASLVLAF 547
           S +    +   +Y+  A+ D      + + C G  C+  +F+    + ++G ++SL L  
Sbjct: 523 SLLYGY-IAASIYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYI 562

BLAST of CsaV3_1G013250.1 vs. TrEMBL
Match: tr|A0A1S3CMR5|A0A1S3CMR5_CUCME (uncharacterized protein LOC103502570 OS=Cucumis melo OX=3656 GN=LOC103502570 PE=4 SV=1)

HSP 1 Score: 1029.2 bits (2660), Expect = 3.6e-297
Identity = 519/594 (87.37%), Postives = 555/594 (93.43%), Query Frame = 0

Query: 3   DKFRPFLKQLATGRWFSVFASFLIMIGAGSTYVFGTYSKAIKTQFDYSQTEINTLGFAKD 62
           + F PF+KQL  GRWFSVFASFLIMIGAGSTYVFGTYSK IKTQF+YSQTEINTLGFAKD
Sbjct: 4   NNFLPFVKQLTAGRWFSVFASFLIMIGAGSTYVFGTYSKVIKTQFNYSQTEINTLGFAKD 63

Query: 63  LGSNLGVFAGLLGEIAPPWVLFVVGSFLNFFSYFMIWLSLTHRIAKPQLWQMFIYICLAA 122
           LGSNLGVFAG LGEIAPPWVL VVG+FLNF+SYFMIWLS+T RIAKPQLWQMF YICLAA
Sbjct: 64  LGSNLGVFAGFLGEIAPPWVLLVVGAFLNFYSYFMIWLSVTRRIAKPQLWQMFFYICLAA 123

Query: 123 NSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAIVTQFYLALHGHDNPASLVLLL 182
           NSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAI+TQF+LAL+GH+NP++LVLLL
Sbjct: 124 NSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQFFLALYGHENPSNLVLLL 183

Query: 183 AWFPTLISSLFFLSIRTINMRRHPEELRVLYHLLYVSIILALFLLFLTVSQKQAAFSSAG 242
           +WFPTLIS  FFLSIRTIN+RRHPEELRVLYHLLYVSIILALFLLFLT++QKQAAFSSAG
Sbjct: 184 SWFPTLISLAFFLSIRTINIRRHPEELRVLYHLLYVSIILALFLLFLTITQKQAAFSSAG 243

Query: 243 YASGAAVIIGLLLMPLLIAVREELMLFKLNGQTDKNSSPAVFTPEMKTSSSSTTKNNESL 302
           Y SGAAVI+GLL +PLLIAVREELMLFKL GQTD N SP VF PEMK+SS+ST KNNESL
Sbjct: 244 YTSGAAVIVGLLSIPLLIAVREELMLFKLKGQTDNNPSPPVFIPEMKSSSNSTPKNNESL 303

Query: 303 SPIEEIPELNSPTCCSNIVNKPERGEDFSILQALFSKDMGLIFVATLCGCGSSIAAIDNI 362
           +PIEEIPE NSPTC SN+ NKPERGEDF+ILQALFSKDMGLI V TL GCGSSIAAIDNI
Sbjct: 304 TPIEEIPETNSPTCLSNVFNKPERGEDFTILQALFSKDMGLILVGTLFGCGSSIAAIDNI 363

Query: 363 GQIGESLGYPSKSISIFVSWVSIFSFFGRVGSGFISETLMTKYKLPRPLMFAFSHLLTCI 422
           GQIGESLGY SKSISIFVSWVSIF+FFGRVGSGFISETLMTKYKLPRPLMFA +H  TCI
Sbjct: 364 GQIGESLGYTSKSISIFVSWVSIFNFFGRVGSGFISETLMTKYKLPRPLMFACAHFFTCI 423

Query: 423 GMLFVAFPYPGSIYVASLTIGFGFGAQVPIIFAILSELFGLKYYATIFNCAQLAVPIGSY 482
           GMLFVAFPYPGSIY ASL IGFGFGAQVP++FAILSELFGLKYYATIFNCAQLAVPIGSY
Sbjct: 424 GMLFVAFPYPGSIYGASLIIGFGFGAQVPMLFAILSELFGLKYYATIFNCAQLAVPIGSY 483

Query: 483 VLNVDVIGKLYDIEATKDGGIRDGNGLTCKGAHCFSGSFLVLAVVVLIGGLASLVLAFRT 542
           +LNVDVIGK YD EATK G  RDG GLTCKG HCFSGSFLVL+VVVLIGG+ASLVLAFRT
Sbjct: 484 ILNVDVIGKFYDHEATKGGSTRDGKGLTCKGTHCFSGSFLVLSVVVLIGGVASLVLAFRT 543

Query: 543 RNFYKGDVYKKYREDMWIPQSDMEFYCLDDKKKVVNDNFPRIVMPPKYNFLNVI 597
           R+FYKGDVYKKYREDMWIPQSDMEFYCLD+KKKVVN+N PR+VMPPKY+FLN+I
Sbjct: 544 RDFYKGDVYKKYREDMWIPQSDMEFYCLDNKKKVVNNNLPRVVMPPKYSFLNII 597

BLAST of CsaV3_1G013250.1 vs. TrEMBL
Match: tr|A0A0A0LUW4|A0A0A0LUW4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G097645 PE=4 SV=1)

HSP 1 Score: 739.6 bits (1908), Expect = 5.6e-210
Identity = 362/574 (63.07%), Postives = 461/574 (80.31%), Query Frame = 0

Query: 8   FLKQLATGRWFSVFASFLIMIGAGSTYVFGTYSKAIKTQFDYSQTEINTLGFAKDLGSNL 67
           F KQ+  GRWFS+FA+FLIMIG GSTY+FGTYSK +KT+FDYSQT++++L FAKDLGSNL
Sbjct: 12  FFKQVLEGRWFSIFAAFLIMIGCGSTYLFGTYSKVLKTKFDYSQTQLSSLSFAKDLGSNL 71

Query: 68  GVFAGLLGEIAPPWVLFVVGSFLNFFSYFMIWLSLTHRIAKPQLWQMFIYICLAANSQNF 127
           GVFAGL  E+APPW+LF+VG  LNFFSYFMIWLSL+  + KP LW MFIY+ ++AN+QNF
Sbjct: 72  GVFAGLFAEVAPPWMLFLVGLTLNFFSYFMIWLSLSEYVPKPNLWLMFIYVYISANAQNF 131

Query: 128 ANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAIVTQFYLALHGHDNPASLVLLLAWFPT 187
           ANTAVLVTSVRNFPD+RG+++GLLKGFVG+GGAI+TQ Y +++GHD+P SLVLLL+W P+
Sbjct: 132 ANTAVLVTSVRNFPDQRGVVIGLLKGFVGLGGAILTQVYFSMYGHDDPISLVLLLSWLPS 191

Query: 188 LISSLFFLSIRTINMRRHPEELRVLYHLLYVSIILALFLLFLTVSQKQAAFSSAGYASGA 247
           L+  LFFL+ RTI   +HP+EL++ +HLLYVS+ +A+F+LFLT++QK + F+ A Y  G 
Sbjct: 192 LVCFLFFLTFRTIKAPKHPQELKIFFHLLYVSLTMAVFILFLTITQKNSHFTHAKYVGGV 251

Query: 248 AVIIGLLLMPLLIAVREELMLFKLNGQTDKNSSPAVFTPEMKTSSSSTTKNNESLSPIEE 307
           +VII LL +PLLIA++EEL LFKLN QT K+ S  V  P +K               +EE
Sbjct: 252 SVIIVLLCLPLLIAIKEELFLFKLNKQT-KDPSVVVSIPVLK---------------LEE 311

Query: 308 IPELNSPTCCS-NIVNKPERGEDFSILQALFSKDMGLIFVATLCGCGSSIAAIDNIGQIG 367
           + E +SP   S N+ NKP+RG+DF ILQALFSKDM LIF+AT+  CGSS+AAIDN+GQI 
Sbjct: 312 VAETSSPPSFSNNVSNKPQRGDDFGILQALFSKDMALIFIATVSACGSSVAAIDNLGQIA 371

Query: 368 ESLGYPSKSISIFVSWVSIFSFFGRVGSGFISETLMTKYKLPRPLMFAFSHLLTCIGMLF 427
           ESL YPSKSI++FVSW+SIF+FFGRV SGFISETLMTKYKLPRPLMF  + ++TCIG++ 
Sbjct: 372 ESLNYPSKSINVFVSWISIFNFFGRVCSGFISETLMTKYKLPRPLMFGLTQIITCIGLVA 431

Query: 428 VAFPYPGSIYVASLTIGFGFGAQVPIIFAILSELFGLKYYATIFNCAQLAVPIGSYVLNV 487
           +AFP+  SIY ASL +GFGFGAQ P++FA++S+LFGLK+Y+T+ NC QLAVP GSY++N+
Sbjct: 432 IAFPFKNSIYAASLIVGFGFGAQTPLLFALISDLFGLKHYSTLLNCGQLAVPFGSYIMNI 491

Query: 488 DVIGKLYDIEATKDGGIRDGNGLTCKGAHCFSGSFLVLAVVVLIGGLASLVLAFRTRNFY 547
            V+GKLYD EATK+G ++ G GLTC G HCFS SF +L +  L G +AS VLA+RTR FY
Sbjct: 492 HVVGKLYDREATKNGNVKTGKGLTCTGIHCFSKSFTILVIATLFGAMASFVLAYRTREFY 551

Query: 548 KGDVYKKYRED-MW-IPQSDMEFYCLDDKKKVVN 579
           KGD+YK+YR+D MW   QSD+E +   D KK+ N
Sbjct: 552 KGDIYKRYRDDQMWTTTQSDVELFSSSDNKKMKN 569

BLAST of CsaV3_1G013250.1 vs. TrEMBL
Match: tr|A0A1S3CMB5|A0A1S3CMB5_CUCME (uncharacterized protein LOC103502564 OS=Cucumis melo OX=3656 GN=LOC103502564 PE=4 SV=1)

HSP 1 Score: 736.9 bits (1901), Expect = 3.7e-209
Identity = 359/573 (62.65%), Postives = 460/573 (80.28%), Query Frame = 0

Query: 8   FLKQLATGRWFSVFASFLIMIGAGSTYVFGTYSKAIKTQFDYSQTEINTLGFAKDLGSNL 67
           F+KQ+  GRWFS+FA+FLIMIG GSTY+FGTYSK +KT+FD++QT++++LGFAKDLG+NL
Sbjct: 12  FVKQVLQGRWFSIFAAFLIMIGCGSTYLFGTYSKVLKTKFDFNQTQLSSLGFAKDLGANL 71

Query: 68  GVFAGLLGEIAPPWVLFVVGSFLNFFSYFMIWLSLTHRIAKPQLWQMFIYICLAANSQNF 127
           GVFAGL  E+APPW+LF+VG  LNFFSYFMIWLSL+  + KP LW MFIYI ++AN+QNF
Sbjct: 72  GVFAGLFAEVAPPWMLFLVGLTLNFFSYFMIWLSLSEYVPKPDLWLMFIYIYISANAQNF 131

Query: 128 ANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAIVTQFYLALHGHDNPASLVLLLAWFPT 187
           ANTAV+VTSVRNFPD+RG++LGLLKGFVG+GGAI+TQ Y +++GH++P SLVLLL+W P+
Sbjct: 132 ANTAVMVTSVRNFPDQRGVVLGLLKGFVGLGGAILTQIYFSIYGHEDPISLVLLLSWLPS 191

Query: 188 LISSLFFLSIRTINMRRHPEELRVLYHLLYVSIILALFLLFLTVSQKQAAFSSAGYASGA 247
           L+  LFFLS RT+  R+HP+EL+V +HLLYVS+ +A F+LFLT++QK   F+ A Y  G 
Sbjct: 192 LVCFLFFLSFRTVKARKHPQELKVFFHLLYVSLTMAAFILFLTITQKSTPFTHAKYVGGV 251

Query: 248 AVIIGLLLMPLLIAVREELMLFKLNGQTDKNSSPAVFTPEMKTSSSSTTKNNESLSPIEE 307
           +VI+ LL +PLLIAV+EEL LFKLN QT K+ S  V  P  K               +EE
Sbjct: 252 SVIVALLSLPLLIAVKEELFLFKLNKQT-KDPSVVVSIPVQK---------------LEE 311

Query: 308 IPEL-NSPTCCSNIVNKPERGEDFSILQALFSKDMGLIFVATLCGCGSSIAAIDNIGQIG 367
           I E  +SP+   N+ NKP+RG+DF ILQAL SKDM LIF+AT+  CGSS+AAIDN+GQI 
Sbjct: 312 IVETSSSPSLSDNLSNKPQRGDDFGILQALLSKDMALIFIATVSACGSSVAAIDNLGQIA 371

Query: 368 ESLGYPSKSISIFVSWVSIFSFFGRVGSGFISETLMTKYKLPRPLMFAFSHLLTCIGMLF 427
           ESL YPSKSIS+FVSW+SIF+FFGRV SGF+SETLMTKYKLPRPL+F  + ++TCIG++ 
Sbjct: 372 ESLNYPSKSISVFVSWISIFNFFGRVCSGFVSETLMTKYKLPRPLIFGLTQIITCIGLVA 431

Query: 428 VAFPYPGSIYVASLTIGFGFGAQVPIIFAILSELFGLKYYATIFNCAQLAVPIGSYVLNV 487
           +AFP+  S+Y ASL IGFGFGAQ P++FA++S+LFGLK+Y+T+ NC QLAVP GSY++N+
Sbjct: 432 IAFPFNNSVYAASLIIGFGFGAQTPLLFALISDLFGLKHYSTLLNCGQLAVPFGSYIMNI 491

Query: 488 DVIGKLYDIEATKDGGIRDGNGLTCKGAHCFSGSFLVLAVVVLIGGLASLVLAFRTRNFY 547
            V+G+LYD EATK G ++ G GLTC G HCFS SF +L +V L G +AS VLA+RT+ FY
Sbjct: 492 HVVGRLYDREATKIGNVKTGKGLTCTGIHCFSESFGILVIVTLFGAMASFVLAYRTKEFY 551

Query: 548 KGDVYKKYRED-MWIPQSDMEFYCLDDKKKVVN 579
           KGD+YK+YR+D MW  QSD+E +   D KK+ N
Sbjct: 552 KGDIYKRYRDDKMWTTQSDVELFSSSDGKKMKN 568

BLAST of CsaV3_1G013250.1 vs. TrEMBL
Match: tr|A0A1S3CMS0|A0A1S3CMS0_CUCME (uncharacterized protein LOC103502580 OS=Cucumis melo OX=3656 GN=LOC103502580 PE=4 SV=1)

HSP 1 Score: 731.5 bits (1887), Expect = 1.5e-207
Identity = 352/569 (61.86%), Postives = 456/569 (80.14%), Query Frame = 0

Query: 8   FLKQLATGRWFSVFASFLIMIGAGSTYVFGTYSKAIKTQFDYSQTEINTLGFAKDLGSNL 67
           F+KQ+  GRWFSV+A  ++MIG G+TY+FGTYSK +KT+FDYSQT++N LGFAKDLGSN+
Sbjct: 8   FIKQVVQGRWFSVYAGMMMMIGNGTTYIFGTYSKVLKTEFDYSQTQVNMLGFAKDLGSNV 67

Query: 68  GVFAGLLGEIAPPWVLFVVGSFLNFFSYFMIWLSLTHRIAKPQLWQMFIYICLAANSQNF 127
           G+ AGLL E  P WVLF +G+F NF  YF IWLS+T RI +P+ WQMF+ +C  +NS N+
Sbjct: 68  GIIAGLLAEFVPTWVLFTIGAFQNFTCYFFIWLSMTRRIPQPEFWQMFLSVCFGSNSSNY 127

Query: 128 ANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAIVTQFYLALHGHDNPASLVLLLAWFPT 187
           ANTA++V S+RNFPDRRGIILGLLKG+VGIGGAI+TQ YL ++G ++P+ LVLL AWFP+
Sbjct: 128 ANTAIMVISLRNFPDRRGIILGLLKGYVGIGGAILTQIYLGIYGPEDPSDLVLLFAWFPS 187

Query: 188 LISSLFFLSIRTINMRRHPEELRVLYHLLYVSIILALFLLFLTVSQKQAAFSSAGYASGA 247
           ++  L   SIR I++R+HPEEL+V YHLLYVSII+A+F+ FL+++QKQ  FS A YASGA
Sbjct: 188 VLILLISTSIRPIHIRKHPEELKVFYHLLYVSIIIAIFIFFLSITQKQVTFSPAAYASGA 247

Query: 248 AVIIGLLLMPLLIAVREELMLFKLNGQTDKNSSPAVFTPEMKTSSSSTTKNNESLSPIEE 307
            V++ LL +PLLI  REE +L+KL  Q + N  P+V    +       T   +S S +EE
Sbjct: 248 YVVVALLCLPLLIVCREEFLLYKLKKQ-NLNRDPSV---ALSIVDQKVTNCPKSFSTLEE 307

Query: 308 IPELNSPTCCSNIVNKPERGEDFSILQALFSKDMGLIFVATLCGCGSSIAAIDNIGQIGE 367
           I E+ SP+C SNI NKP+RG DF++LQA+FS DM LI +AT  GCGSS+AAIDN+GQIGE
Sbjct: 308 IAEI-SPSCISNICNKPDRGNDFTVLQAIFSIDMLLICLATFSGCGSSLAAIDNLGQIGE 367

Query: 368 SLGYPSKSISIFVSWVSIFSFFGRVGSGFISETLMTKYKLPRPLMFAFSHLLTCIGMLFV 427
           SLGYP+++ISIFVSWVSIF+FFGRV SGFISE ++ KYKLPRPLMFAF+ LLTC+G L +
Sbjct: 368 SLGYPARAISIFVSWVSIFNFFGRVFSGFISELMIIKYKLPRPLMFAFAFLLTCLGQLCI 427

Query: 428 AFPYPGSIYVASLTIGFGFGAQVPIIFAILSELFGLKYYATIFNCAQLAVPIGSYVLNVD 487
           A+P+PGS+YVAS  IGFGFGAQ P++FA++SE+FGLK+Y+ +FNC QL VP+GSY+LNVD
Sbjct: 428 AYPFPGSVYVASSIIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLVVPLGSYILNVD 487

Query: 488 VIGKLYDIEATKDGGIRDGNGLTCKGAHCFSGSFLVLAVVVLIGGLASLVLAFRTRNFYK 547
           ++GKLYD EA ++G    G G+TC GAHCF GSF +LA   L G L  L+LA+RTR++Y+
Sbjct: 488 IVGKLYDAEALREGRKMTGKGITCTGAHCFGGSFTILAASTLFGALVMLLLAYRTRDYYR 547

Query: 548 GDVYKKYREDMWIPQSDMEFYCLDDKKKV 577
            D+YK Y+EDMWIPQ++MEFY LD++K +
Sbjct: 548 WDIYKNYKEDMWIPQAEMEFYRLDNRKNI 571

BLAST of CsaV3_1G013250.1 vs. TrEMBL
Match: tr|A0A1S3CM93|A0A1S3CM93_CUCME (uncharacterized protein LOC103502588 OS=Cucumis melo OX=3656 GN=LOC103502588 PE=4 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 3.2e-197
Identity = 346/574 (60.28%), Postives = 439/574 (76.48%), Query Frame = 0

Query: 1   MADKFRPFLKQLATGRWFSVFASFLIMIGAGSTYVFGTYSKAIKTQFDYSQTEINTLGFA 60
           M  K   + KQ+  GRWFSVFA  ++M+G GSTY++GTYSK IKT+F+YSQT+++ LGFA
Sbjct: 1   MGTKAWDYTKQVIQGRWFSVFAGLIMMVGNGSTYIYGTYSKVIKTEFNYSQTQLSILGFA 60

Query: 61  KDLGSNLGVFAGLLGEIAPPWVLFVVGSFLNFFSYFMIWLSLTHRIAKPQLWQMFIYICL 120
           KDLGSN+G+FAGLL E+APPWVLF++GS  NF  +F+IWLS+THRI +P+ WQMF+ +CL
Sbjct: 61  KDLGSNVGIFAGLLAEVAPPWVLFLIGSVQNFTGFFLIWLSITHRIPQPKFWQMFLCVCL 120

Query: 121 AANSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAIVTQFYLALHGHDNPASLVL 180
             +S NFANTA++VTSV NFPDRRGIILGLL G+VGIGGAI+TQ YL ++G  +P++LVL
Sbjct: 121 GTHSSNFANTAIMVTSVVNFPDRRGIILGLLNGYVGIGGAILTQIYLGIYGPKDPSNLVL 180

Query: 181 LLAWFPTLISSLFFLSIRTINMRRHPEELRVLYHLLYVSIILALFLLFLTVSQKQAAFSS 240
           L AW P+ +  +   SIR I + +HPEEL+V YHLLYVS+ILA+F+LFLT++QK+ AF+ 
Sbjct: 181 LFAWLPSAVILVLSFSIRPICIHKHPEELKVFYHLLYVSVILAMFILFLTIAQKEVAFTR 240

Query: 241 AGYASGAAVIIGLLLMPLLIAVREELMLFKLNGQTDKNSSPAVFTPEMKTSSSSTTKNNE 300
            GY +GAAVI+ LL +PL+I  REE +L+KLN Q + +S         K  SS TT+  E
Sbjct: 241 RGYTNGAAVIVVLLFLPLVIVCREEHVLYKLNKQNEDSSFNISINDHQKPHSSITTEKTE 300

Query: 301 SLSPIEEIPELNSPTCCSNIVNKPERGEDFSILQALFSKDMGLIFVATLCGCGSSIAAID 360
                      NS +  S I NKPERGEDFSILQA+FS DM LIF+AT  G GS++ AID
Sbjct: 301 -----------NSRSYFSKIWNKPERGEDFSILQAIFSIDMTLIFLATFSGSGSALTAID 360

Query: 361 NIGQIGESLGYPSKSISIFVSWVSIFSFFGRVGSGFISETLMTKYKLPRPLMFAFSHLLT 420
           N+GQ+ ESL YPS++ISI VSWVS+F+FFGR+ SGFISE  M KYKLPRPL FA +  +T
Sbjct: 361 NLGQVAESLAYPSEAISIIVSWVSVFNFFGRIFSGFISENFMIKYKLPRPLTFAVAFFIT 420

Query: 421 CIGMLFVAFPYPGSIYVASLTIGFGFGAQVPIIFAILSELFGLKYYATIFNCAQLAVPIG 480
            IG L VA+P  GS+++AS+ IGFGFG QVP++F I+SELFGLK+Y+T+FNC QL VPIG
Sbjct: 421 GIGQLIVAYPSSGSVFIASIVIGFGFGMQVPLLFTIISELFGLKHYSTLFNCGQLVVPIG 480

Query: 481 SYVLNVDVIGKLYDIEATKDGGIRDGNGLTCKGAHCFSGSFLVLAVVVLIGGLASLVLAF 540
           SY+LNVDV+G++YD EA  DG    G GL C GAHCF+GSF +L+   L G +  L LA+
Sbjct: 481 SYILNVDVVGRIYDKEALVDGIKLTGRGLICTGAHCFNGSFTILSGATLFGAIVMLALAY 540

Query: 541 RTRNFYKGDVYKKYREDMWIPQSDMEFYCLDDKK 575
           RTR FYKGDVYKKYR+D+WI  +DMEFY LD KK
Sbjct: 541 RTREFYKGDVYKKYRDDVWITPTDMEFYHLDKKK 563

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004147309.10.0e+00100.00PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis sativus][more]
XP_008464767.15.4e-29787.37PREDICTED: uncharacterized protein LOC103502570 [Cucumis melo][more]
XP_023521059.11.1e-25475.00uncharacterized protein LOC111784657 [Cucurbita pepo subsp. pepo][more]
XP_022964772.13.3e-25474.83uncharacterized protein LOC111464772 [Cucurbita moschata][more]
XP_022970238.14.8e-25374.33uncharacterized protein LOC111469246 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G28120.12.5e-16352.42Major facilitator superfamily protein[more]
AT2G39210.13.6e-14647.50Major facilitator superfamily protein[more]
AT1G74780.18.2e-8235.79Nodulin-like / Major Facilitator Superfamily protein[more]
AT1G18940.12.3e-7634.00Nodulin-like / Major Facilitator Superfamily protein[more]
AT5G50630.12.3e-7633.33Major facilitator superfamily protein[more]
Match NameE-valueIdentityDescription
sp|F4I9E1|NFD4_ARATH5.5e-2726.19Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3CMR5|A0A1S3CMR5_CUCME3.6e-29787.37uncharacterized protein LOC103502570 OS=Cucumis melo OX=3656 GN=LOC103502570 PE=... [more]
tr|A0A0A0LUW4|A0A0A0LUW4_CUCSA5.6e-21063.07Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G097645 PE=4 SV=1[more]
tr|A0A1S3CMB5|A0A1S3CMB5_CUCME3.7e-20962.65uncharacterized protein LOC103502564 OS=Cucumis melo OX=3656 GN=LOC103502564 PE=... [more]
tr|A0A1S3CMS0|A0A1S3CMS0_CUCME1.5e-20761.86uncharacterized protein LOC103502580 OS=Cucumis melo OX=3656 GN=LOC103502580 PE=... [more]
tr|A0A1S3CM93|A0A1S3CM93_CUCME3.2e-19760.28uncharacterized protein LOC103502588 OS=Cucumis melo OX=3656 GN=LOC103502588 PE=... [more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR036259MFS_trans_sf
IPR020846MFS_dom
IPR010658Nodulin-like
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CsaV3_1G013250CsaV3_1G013250gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CsaV3_1G013250.1.cds1CsaV3_1G013250.1.cds1CDS


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CsaV3_1G013250.1.exon1CsaV3_1G013250.1.exon1exon


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CsaV3_1G013250.1CsaV3_1G013250.1-proteinpolypeptide


Analysis Name: InterPro Annotations of cucumber chineselong genome (v3)
Date Performed: 2019-03-04
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1250.20coord: 330..545
e-value: 6.9E-11
score: 43.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 280..302
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 7..566
NoneNo IPR availablePANTHERPTHR21576:SF76SUBFAMILY NOT NAMEDcoord: 7..566
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 16..261
e-value: 1.5E-76
score: 257.1
IPR020846Major facilitator superfamily domainCDDcd06174MFScoord: 365..539
e-value: 1.65113E-4
score: 41.9157
IPR036259MFS transporter superfamilySUPERFAMILYSSF103473MFS general substrate transportercoord: 14..219
coord: 322..544