Csa6G495740.1 (mRNA) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa6G495740.1
Type	mRNA
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	N-acetylglutamate synthase
Location	Chr6 : 24069291 .. 24069944 (-)
Sequence length	453

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CCACTCCACAATTCCTTTAAACATTTTTTTACTCTTTTATCACTTCTTTTCTTCCCACCCCACCGTTTCGCCGCGTTCGCCATTCAGCTTCTTCCTCTCTCTTCTTCCTGCCTTTCTCTGTTTCTGTTTCACAATACACTCTTTAGAAAACCTTTTTCTTTTCCTTCTGGATTCTGTTTCACTTCCATTAAACTGGTGGTTATGGCTACTCCCACAACAAGGACTTGGGTTTTTCCGAAGAGTAGTTACCGTGAAATTACTTGCTTTGATTTTGAGTCGGTGGGTGTGGGACGTGGGTACAGAAAGAATCTGCGGCGATGGACGACGATCAAAGGTCCAATGTGCTGTGAGAGTGGGAAAAGAAAATTGAATATGGGATTGTCGGCGGGGAAAGTCTGCAACCGGGAGGAGGTCAAAACAACCGCTGAGGGGCCGGAAATTGAGACTAAAAATGATGCGTATTATACAAAAGTTACTGAAGAAGACAGGAGATTTATTGAAGAATTCAGAGAAGCTTATCCCTATATTTTTTCTCATATAGGCCGTACTTTTGTCGTCGTTATATCCGCCGAAATTGTAGCCAGTCCTCGCCTCCATGCCTTGCTTAAGGTCTCTCTTTCTCTCGTCTCTGTAATAAAAATTGCTTCATTTTAA

mRNA sequence

ATGGCTACTCCCACAACAAGGACTTGGGTTTTTCCGAAGAGTAGTTACCGTGAAATTACTTGCTTTGATTTTGAGTCGGTGGGTGTGGGACGTGGGTACAGAAAGAATCTGCGGCGATGGACGACGATCAAAGGTCCAATGTGCTGTGAGAGTGGGAAAAGAAAATTGAATATGGGATTGTCGGCGGGGAAAGTCTGCAACCGGGAGGAGGTCAAAACAACCGCTGAGGGGCCGGAAATTGAGACTAAAAATGATGCGTATTATACAAAAGTTACTGAAGAAGACAGGAGATTTATTGAAGAATTCAGAGAAGCTTATCCCTATATTTTTTCTCATATAGGCCGTACTTTTGTCGTCGTTATATCCGCCGAAATTGTAGCCAGTCCTCGCCTCCATGCCTTGCTTAAGGTCTCTCTTTCTCTCGTCTCTGTAATAAAAATTGCTTCATTTTAA

Coding sequence (CDS)

ATGGCTACTCCCACAACAAGGACTTGGGTTTTTCCGAAGAGTAGTTACCGTGAAATTACTTGCTTTGATTTTGAGTCGGTGGGTGTGGGACGTGGGTACAGAAAGAATCTGCGGCGATGGACGACGATCAAAGGTCCAATGTGCTGTGAGAGTGGGAAAAGAAAATTGAATATGGGATTGTCGGCGGGGAAAGTCTGCAACCGGGAGGAGGTCAAAACAACCGCTGAGGGGCCGGAAATTGAGACTAAAAATGATGCGTATTATACAAAAGTTACTGAAGAAGACAGGAGATTTATTGAAGAATTCAGAGAAGCTTATCCCTATATTTTTTCTCATATAGGCCGTACTTTTGTCGTCGTTATATCCGCCGAAATTGTAGCCAGTCCTCGCCTCCATGCCTTGCTTAAGGTCTCTCTTTCTCTCGTCTCTGTAATAAAAATTGCTTCATTTTAA

Protein sequence

MATPTTRTWVFPKSSYREITCFDFESVGVGRGYRKNLRRWTTIKGPMCCESGKRKLNMGLSAGKVCNREEVKTTAEGPEIETKNDAYYTKVTEEDRRFIEEFREAYPYIFSHIGRTFVVVISAEIVASPRLHALLKVSLSLVSVIKIASF*

Homology

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BLAST of Csa6G495740.1 vs. Swiss-Prot
Match: NAGS2_ARATH (Probable amino-acid acetyltransferase NAGS2, chloroplastic OS=Arabidopsis thaliana GN=NAGS2 PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 1.8e-06
Identity = 28/71 (39.44%), Postives = 42/71 (59.15%), Query Frame = 1


Query: 66  CNREEVKTTAEGPEIETKNDAYYTKVTEEDRRFIEEFREAYPYIFSHIGRTFVVVISAEI 125
           CN  +   TA G ++E ++         +D++F+  FREA+PY+++H G TFVV+IS EI
Sbjct: 60  CNMFDYAVTAAG-DVEAEHPV-------DDKQFVRWFREAWPYLWAHRGCTFVVIISGEI 119

Query: 126 VASPRLHALLK 137
           +A     A+LK
Sbjct: 120 IAGSSCDAILK 122

[Close]

BLAST of Csa6G495740.1 vs. TrEMBL
Match: A0A0A0KFZ3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G495740 PE=4 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 4.9e-80
Identity = 150/150 (100.00%), Postives = 150/150 (100.00%), Query Frame = 1


Query: 1   MATPTTRTWVFPKSSYREITCFDFESVGVGRGYRKNLRRWTTIKGPMCCESGKRKLNMGL 60
           MATPTTRTWVFPKSSYREITCFDFESVGVGRGYRKNLRRWTTIKGPMCCESGKRKLNMGL
Sbjct: 1   MATPTTRTWVFPKSSYREITCFDFESVGVGRGYRKNLRRWTTIKGPMCCESGKRKLNMGL 60

Query: 61  SAGKVCNREEVKTTAEGPEIETKNDAYYTKVTEEDRRFIEEFREAYPYIFSHIGRTFVVV 120
           SAGKVCNREEVKTTAEGPEIETKNDAYYTKVTEEDRRFIEEFREAYPYIFSHIGRTFVVV
Sbjct: 61  SAGKVCNREEVKTTAEGPEIETKNDAYYTKVTEEDRRFIEEFREAYPYIFSHIGRTFVVV 120

Query: 121 ISAEIVASPRLHALLKVSLSLVSVIKIASF 151
           ISAEIVASPRLHALLKVSLSLVSVIKIASF
Sbjct: 121 ISAEIVASPRLHALLKVSLSLVSVIKIASF 150

[Close]

BLAST of Csa6G495740.1 vs. TrEMBL
Match: H6V8U2_9ROSI (N-acetylglutamate synthase OS=Cucurbita maxima x Cucurbita moschata GN=NAGS PE=2 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 1.5e-33
Identity = 86/136 (63.24%), Postives = 100/136 (73.53%), Query Frame = 1


Query: 1   MATPTTRTWVFPKSSYREITCFDFESVGVGRGYRKNLRRWTTIKGPMCCESGKRKLNMGL 60
           MATPTTRTWVFP S++ +I   DF++VGV RG R+N  R  T++ P C E+G+RK N GL
Sbjct: 1   MATPTTRTWVFPNSNHWKIWS-DFKAVGVERGCRENYLR-RTVQDPTCHENGRRKWNWGL 60

Query: 61  SAGKVCNREEVKTTAEGPEIETKNDAYYTKVTEEDRRFIEEFREAYPYIFSHIGRTFVVV 120
           +A +            G   E KNDAY  KV EEDRRFIEEFR+AYPYIFSHIGRTFVVV
Sbjct: 61  AAVR-----------GGKRGEAKNDAY-DKVPEEDRRFIEEFRDAYPYIFSHIGRTFVVV 120

Query: 121 ISAEIVASPRLHALLK 137
           ISAEIVASPRLHALL+
Sbjct: 121 ISAEIVASPRLHALLE 122

[Close]

BLAST of Csa6G495740.1 vs. TrEMBL
Match: W9RJH1_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_009378 PE=4 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.2e-09
Identity = 34/48 (70.83%), Postives = 41/48 (85.42%), Query Frame = 1


Query: 91  VTEEDRRFIEEFREAYPYIFSHIGRTFVVVISAEIVASPRLHALLKVS 139
           V+EEDR F+E  REA PYIF+HIGRTFVVV+SAEIVA P L ++LKV+
Sbjct: 75  VSEEDRSFVEALREAQPYIFAHIGRTFVVVLSAEIVAGPCLDSILKVT 122

[Close]

BLAST of Csa6G495740.1 vs. TrEMBL
Match: A0A059ALL7_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_I00696 PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 2.5e-07
Identity = 49/146 (33.56%), Postives = 70/146 (47.95%), Query Frame = 1


Query: 1   MATPTTRTWVFPKSSYREI-------TCFD--FESVGVGRGYRKNLRRWTTIKGPMCCES 60
           MAT  +R W+  +S + E        +C      S G   G +  L +   I G +    
Sbjct: 1   MATSNSRPWICTRSHHEEHCPANRRRSCQQTHHRSGGELSGGKARLLK---IPGGVGLRV 60

Query: 61  GKRKLNMGLSAGKV-CNREEVKTTAEGPEIETKNDAYYTKVTEEDRRFIEEFREAYPYIF 120
           G RK     +A +  C R++             N   Y + TEE R F+++ REA PYI 
Sbjct: 61  GGRKKGAAAAAAQCGCRRDD----------SGGNWYRYRRTTEEQRGFVDDIREAQPYIH 120

Query: 121 SHIGRTFVVVISAEIVASPRLHALLK 137
           +H GRTF+VV+S EIVA P L ++LK
Sbjct: 121 AHRGRTFIVVLSGEIVAGPCLDSILK 133

[Close]

BLAST of Csa6G495740.1 vs. TrEMBL
Match: A0A061DP53_THECC (N-acetyl-l-glutamate synthase 1 OS=Theobroma cacao GN=TCM_000809 PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.2e-06
Identity = 39/105 (37.14%), Postives = 57/105 (54.29%), Query Frame = 1


Query: 32  GYRKNLRRWTTIKGPMCCESGKRKLNMGLSAGKVCNREEVKTTAEGPEIETKNDAYYTKV 91
           G++K + +   +K  + C +   K   G   G    R  +    E    ET N       
Sbjct: 26  GFKKGVVK---LKPDLKCRAQSLKPEPGSKRGDSVKRNVIND--EDSVEETYN------- 85

Query: 92  TEEDRRFIEEFREAYPYIFSHIGRTFVVVISAEIVASPRLHALLK 137
           T +D++F+  FREA+PY+++H G TFVV+IS EIVASP L A+LK
Sbjct: 86  TVDDKQFVRWFREAWPYLWAHRGSTFVVIISGEIVASPSLDAILK 118

[Close]

BLAST of Csa6G495740.1 vs. TAIR10
Match: AT4G37670.2 (AT4G37670.2 N-acetyl-l-glutamate synthase 2)

HSP 1 Score: 53.9 bits (128), Expect = 9.9e-08
Identity = 28/71 (39.44%), Postives = 42/71 (59.15%), Query Frame = 1


Query: 66  CNREEVKTTAEGPEIETKNDAYYTKVTEEDRRFIEEFREAYPYIFSHIGRTFVVVISAEI 125
           CN  +   TA G ++E ++         +D++F+  FREA+PY+++H G TFVV+IS EI
Sbjct: 60  CNMFDYAVTAAG-DVEAEHPV-------DDKQFVRWFREAWPYLWAHRGCTFVVIISGEI 119

Query: 126 VASPRLHALLK 137
           +A     A+LK
Sbjct: 120 IAGSSCDAILK 122

[Close]

BLAST of Csa6G495740.1 vs. NCBI nr
Match: gi|700193416|gb|KGN48620.1| (hypothetical protein Csa_6G495740 [Cucumis sativus])

HSP 1 Score: 305.1 bits (780), Expect = 7.0e-80
Identity = 150/150 (100.00%), Postives = 150/150 (100.00%), Query Frame = 1


Query: 1   MATPTTRTWVFPKSSYREITCFDFESVGVGRGYRKNLRRWTTIKGPMCCESGKRKLNMGL 60
           MATPTTRTWVFPKSSYREITCFDFESVGVGRGYRKNLRRWTTIKGPMCCESGKRKLNMGL
Sbjct: 1   MATPTTRTWVFPKSSYREITCFDFESVGVGRGYRKNLRRWTTIKGPMCCESGKRKLNMGL 60

Query: 61  SAGKVCNREEVKTTAEGPEIETKNDAYYTKVTEEDRRFIEEFREAYPYIFSHIGRTFVVV 120
           SAGKVCNREEVKTTAEGPEIETKNDAYYTKVTEEDRRFIEEFREAYPYIFSHIGRTFVVV
Sbjct: 61  SAGKVCNREEVKTTAEGPEIETKNDAYYTKVTEEDRRFIEEFREAYPYIFSHIGRTFVVV 120

Query: 121 ISAEIVASPRLHALLKVSLSLVSVIKIASF 151
           ISAEIVASPRLHALLKVSLSLVSVIKIASF
Sbjct: 121 ISAEIVASPRLHALLKVSLSLVSVIKIASF 150

[Close]

BLAST of Csa6G495740.1 vs. NCBI nr
Match: gi|449448208|ref|XP_004141858.1| (PREDICTED: probable amino-acid acetyltransferase NAGS2, chloroplastic isoform X1 [Cucumis sativus])

HSP 1 Score: 282.3 bits (721), Expect = 4.8e-73
Identity = 136/136 (100.00%), Postives = 136/136 (100.00%), Query Frame = 1


Query: 1   MATPTTRTWVFPKSSYREITCFDFESVGVGRGYRKNLRRWTTIKGPMCCESGKRKLNMGL 60
           MATPTTRTWVFPKSSYREITCFDFESVGVGRGYRKNLRRWTTIKGPMCCESGKRKLNMGL
Sbjct: 1   MATPTTRTWVFPKSSYREITCFDFESVGVGRGYRKNLRRWTTIKGPMCCESGKRKLNMGL 60

Query: 61  SAGKVCNREEVKTTAEGPEIETKNDAYYTKVTEEDRRFIEEFREAYPYIFSHIGRTFVVV 120
           SAGKVCNREEVKTTAEGPEIETKNDAYYTKVTEEDRRFIEEFREAYPYIFSHIGRTFVVV
Sbjct: 61  SAGKVCNREEVKTTAEGPEIETKNDAYYTKVTEEDRRFIEEFREAYPYIFSHIGRTFVVV 120

Query: 121 ISAEIVASPRLHALLK 137
           ISAEIVASPRLHALLK
Sbjct: 121 ISAEIVASPRLHALLK 136

[Close]

BLAST of Csa6G495740.1 vs. NCBI nr
Match: gi|659079721|ref|XP_008440407.1| (PREDICTED: probable amino-acid acetyltransferase NAGS2, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 248.4 bits (633), Expect = 7.8e-63
Identity = 124/136 (91.18%), Postives = 128/136 (94.12%), Query Frame = 1


Query: 1   MATPTTRTWVFPKSSYREITCFDFESVGVGRGYRKNLRRWTTIKGPMCCESGKRKLNMGL 60
           MATPTTRTWVFPKS++RE T FDF SVGVGRG RKNLRRWT IKGPM CESG+RKLN GL
Sbjct: 1   MATPTTRTWVFPKSNHREDTWFDFMSVGVGRGCRKNLRRWT-IKGPMRCESGRRKLNTGL 60

Query: 61  SAGKVCNREEVKTTAEGPEIETKNDAYYTKVTEEDRRFIEEFREAYPYIFSHIGRTFVVV 120
           +AGKVCNREEVKTTAEGPEIETKNDAYY KVTEEDRRFIEEFREAYPYIFSHIGRTFVVV
Sbjct: 61  AAGKVCNREEVKTTAEGPEIETKNDAYYNKVTEEDRRFIEEFREAYPYIFSHIGRTFVVV 120

Query: 121 ISAEIVASPRLHALLK 137
           ISAEIVASPRLHALLK
Sbjct: 121 ISAEIVASPRLHALLK 135

[Close]

BLAST of Csa6G495740.1 vs. NCBI nr
Match: gi|375155311|gb|AFA37979.1| (N-acetylglutamate synthase [Cucurbita maxima x Cucurbita moschata])

HSP 1 Score: 150.6 bits (379), Expect = 2.2e-33
Identity = 86/136 (63.24%), Postives = 100/136 (73.53%), Query Frame = 1


Query: 1   MATPTTRTWVFPKSSYREITCFDFESVGVGRGYRKNLRRWTTIKGPMCCESGKRKLNMGL 60
           MATPTTRTWVFP S++ +I   DF++VGV RG R+N  R  T++ P C E+G+RK N GL
Sbjct: 1   MATPTTRTWVFPNSNHWKIWS-DFKAVGVERGCRENYLR-RTVQDPTCHENGRRKWNWGL 60

Query: 61  SAGKVCNREEVKTTAEGPEIETKNDAYYTKVTEEDRRFIEEFREAYPYIFSHIGRTFVVV 120
           +A +            G   E KNDAY  KV EEDRRFIEEFR+AYPYIFSHIGRTFVVV
Sbjct: 61  AAVR-----------GGKRGEAKNDAY-DKVPEEDRRFIEEFRDAYPYIFSHIGRTFVVV 120

Query: 121 ISAEIVASPRLHALLK 137
           ISAEIVASPRLHALL+
Sbjct: 121 ISAEIVASPRLHALLE 122

[Close]

BLAST of Csa6G495740.1 vs. NCBI nr
Match: gi|703123164|ref|XP_010102750.1| (hypothetical protein L484_009378 [Morus notabilis])

HSP 1 Score: 71.2 bits (173), Expect = 1.7e-09
Identity = 34/48 (70.83%), Postives = 41/48 (85.42%), Query Frame = 1


Query: 91  VTEEDRRFIEEFREAYPYIFSHIGRTFVVVISAEIVASPRLHALLKVS 139
           V+EEDR F+E  REA PYIF+HIGRTFVVV+SAEIVA P L ++LKV+
Sbjct: 75  VSEEDRSFVEALREAQPYIFAHIGRTFVVVLSAEIVAGPCLDSILKVT 122

The following BLAST results are available for this feature:

BLAST of Csa6G495740.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
NAGS2_ARATH	1.8e-06	39.44	Probable amino-acid acetyltransferase NAGS2, chloroplastic OS=Arabidopsis thalia...	[more]

BLAST of Csa6G495740.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KFZ3_CUCSA	4.9e-80	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G495740 PE=4 SV=1	[more]
H6V8U2_9ROSI	1.5e-33	63.24	N-acetylglutamate synthase OS=Cucurbita maxima x Cucurbita moschata GN=NAGS PE=2...	[more]
W9RJH1_9ROSA	1.2e-09	70.83	Uncharacterized protein OS=Morus notabilis GN=L484_009378 PE=4 SV=1	[more]
A0A059ALL7_EUCGR	2.5e-07	33.56	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_I00696 PE=3 SV=1	[more]
A0A061DP53_THECC	1.2e-06	37.14	N-acetyl-l-glutamate synthase 1 OS=Theobroma cacao GN=TCM_000809 PE=3 SV=1	[more]

BLAST of Csa6G495740.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G37670.2	9.9e-08	39.44	N-acetyl-l-glutamate synthase 2	[more]

BLAST of Csa6G495740.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700193416\|gb\|KGN48620.1\|	7.0e-80	100.00	hypothetical protein Csa_6G495740 [Cucumis sativus]	[more]
gi\|449448208\|ref\|XP_004141858.1\|	4.8e-73	100.00	PREDICTED: probable amino-acid acetyltransferase NAGS2, chloroplastic isoform X1...	[more]
gi\|659079721\|ref\|XP_008440407.1\|	7.8e-63	91.18	PREDICTED: probable amino-acid acetyltransferase NAGS2, chloroplastic isoform X1...	[more]
gi\|375155311\|gb\|AFA37979.1\|	2.2e-33	63.24	N-acetylglutamate synthase [Cucurbita maxima x Cucurbita moschata]	[more]
gi\|703123164\|ref\|XP_010102750.1\|	1.7e-09	70.83	hypothetical protein L484_009378 [Morus notabilis]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042967	acyl-carrier-protein biosynthetic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0008080	N-acetyltransferase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Csa6G495740	Csa6G495740	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Csa6G495740.1	Csa6G495740.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csa6G495740.1.cds1	Csa6G495740.1.cds1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csa6G495740.1.utr5p1	Csa6G495740.1.utr5p1	five_prime_UTR

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR30602	AMINO-ACID ACETYLTRANSFERASE	coord: 93..136 score: 1.8
None	No IPR available	PANTHER	PTHR30602:SF4	AMINO-ACID ACETYLTRANSFERASE NAGS1, CHLOROPLASTIC-RELATED	coord: 93..136 score: 1.8