Csa5G622560.2 (mRNA) Cucumber (Chinese Long) v2

NameCsa5G622560.2
TypemRNA
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionPentatricopeptide repeat-containing protein; contains IPR002885 (Pentatricopeptide repeat), IPR011990 (Tetratricopeptide-like helical)
LocationChr5 : 24597716 .. 24600365 (+)
Sequence length1557
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGTCAGCAAGGGTATAATCTTGAAGCACTGGTTATATTCTCAAAGATGTTGAATGAACCTTCTTTAAAGCCAGATAACTTCACCTTGGCAAGCATTTTATCAGCTTGTGCCAATCTTGAGAAGCTGAATATTGGGAAACAGATACATGCTTATATTCTAAGAGCAGAAACTGAAACTTCTGGAGCAGTTGGCAATGCTTTGATATCAATGTATGCAAAGTCAGGTGGTGTAGAAATAGCTAGACTGATAGTTGAGCATAACAGAACTTCAAATCTAAACATTATAGCGTTCACTTCCCTACTGGATGGATATACCAAGCTAGGAAATGTCAAACCAGCCAGAGAGATATTTAACAAATTAAGAGATCGTGATGTGGTAGCATGGACAGCCATGATTGTTGGTTATGTGCAAAACGGGTTATGGAATGATGCACTGGAGCTGTTTAGGTTGATGGTTAATGAAGGCCCTGAGCCGAATAGTTATACCCTGGCGGCCATGCTTAGTGTAAGTTCAAGTTTGACTATTTTGGAGCATGGGAAACAAATCCATGCCAGTGCCATAAAAGCTGGAGAATCCTCGACACCTTCTGTTACTAATGCCTTGATAGCCATGTATGCTAAAACGGGAAACATCAATGTAGCCAAAAGAGTTTTTGATCTTCCAAATGGGAAGAAGGAAATTGTGTCCTGGACATCAATGATCATGGCTCTCGCACAGCATGGCCTTGGAAAAGAAGCCATCAACCTGTTTGAGAGGATGCTCTCAGTTGGTATGAAACCTGATCATATCACTTATGTAGGAGTGCTCTCTGCTTGTACACATGTGGGATTAGTGGAGCAAGGTCGAAAGTACTATAATATGATGACTGAAGTTCATGAAATTGAACCCACTCTAAGCCATTATGCATGCATGATCGACCTTTATGGACGGGCTGGATTGCTTCAAGAAGCATACCTGTTCATTGAAAGCATGCCTATAGAACCAGATAATATAGCTTGGGGTTCGCTACTAGCATCTTGTAAAATTCATAAAAATGCAGATCTGGCAAAAGTTGCAGCAGAAAGACTGCTTCTTATTGATCCTGGAAATAGTGGCGCCTACTTGGCACTTGCTAATGTGTATTCGGCTTGCGGAAAATGGGAAAATGCTGCCCAAACTAGAAAACTAATGAAGGATAGAGGAGTGAGGAAAGAGAAGGGAATTAGTTGGATTCATATTAAGAATGAAGTTCATGCATTTGGAGTTGAAGACGTTATTCATCCACAGAAAGATGAAATCTACAAGTTGATGGCTGAGATATGGGAGGAGATAAAAAAGATGGGTTTCATCCCAGACACTGAATCAGTACTTCATGACCTTGAAGAAGAGGTGAAAGAGCAAATACTTAAATATCACAGTGAAAAACTTGCTATAGCGTTTGGGCTTTTAAATACTCCAGAGAACACTGCTCTGAGGATTATGAAAAACCTTAGAGTTTGTAATGACTGCCATTCTGCTATAAAGTTCATCTCCAAGCTTGTAGGAAGGGAAATCATTGTGAGAGATGCTACTCGGTTCCACCATTTTAAGGATGGCTCTTGCTCTTGTCGAGACTATTGGTAGGTGTAAGTTTTTAGAGATTTTATCCACAAAAGAATTCTACTTGATACGAAAAAAGCACTCTCCAAGACCAAGTGTCAGAAAGCCAGGTATGTTCTTCCCCTATTGCTCTTTCCAATGTTTGATTTTATTCTAGAAATCCTTCTAAATCAACCTTACAAATTAGAAAAGAAAAAACTTGGATGCTCTGTATACATCTTTCTGTAATCTATCTAGTTGTTCATCACAATTTACCAAGGAGTGAACATTTCTTTCTTCTTTTTCTTTTTTGAACGCTTTGTGTGTTGGTGTGGTTGGTGTGGGCTGCAAAAGGATGATGCATCCACGATGCACCCAAGTTTTACTCTACAAAGTAGTATATTCTCTATTCATTAGGGGGTGGGGAAAAGATTGTTTGATCTACTAATCAAACAAGATCATTTCTTCTTTTTCTACATCATTCCCATCCTTGACTACAGAATATCCATTAGGTAATAGTTCGTACCCAAAAACATATTTAAAAAATCAAATTTCTTTATGGTTTATTGAACTGTATCCTTTCTTTCAAATTTGATCCCGTTCCATTTTGTTTTGTTTTGTTTTTTATTTTCAAGGACTTGCATAACAGAGTTATAAAGTACGAGTCCCCATCTTATCCCAGACAATGTGTTCTTGACCTCAACTGGTTGTAGGGCGCCAAACTTCCAATTTCATTTTGAAGAGAATGCAAACCTACAACAAGCCTATTTGTGATATATGCATCTTAGAGAAGATTGTAAGTAAAACTGGTTGAATTATAGATCATATAGACTTTGTTTTTAATCCCTCTTTTCCATTTTTATCAGAAACTATATTCATAATTTCTTCCACAAATATATACACATCCAACTTTGACTCAATATCTACTTAATTATATTGCAGTGGAAGATGCAAGGCTTGATGTGGCTAATTTACTGGGAGCACTCAGAATGAACGAGTATTAAAGGTGCATGTTTAAAGGAATCTCTCTATAATTTTAAGTATGTAGTATGTATACAAAGTGTCAATTTCTTTCACTACTCTTGCATAATTT

mRNA sequence

ATGTTGAATGAACCTTCTTTAAAGCCAGATAACTTCACCTTGGCAAGCATTTTATCAGCTTGTGCCAATCTTGAGAAGCTGAATATTGGGAAACAGATACATGCTTATATTCTAAGAGCAGAAACTGAAACTTCTGGAGCAGTTGGCAATGCTTTGATATCAATGTATGCAAAGTCAGGTGGTGTAGAAATAGCTAGACTGATAGTTGAGCATAACAGAACTTCAAATCTAAACATTATAGCGTTCACTTCCCTACTGGATGGATATACCAAGCTAGGAAATGTCAAACCAGCCAGAGAGATATTTAACAAATTAAGAGATCGTGATGTGGTAGCATGGACAGCCATGATTGTTGGTTATGTGCAAAACGGGTTATGGAATGATGCACTGGAGCTGTTTAGGTTGATGGTTAATGAAGGCCCTGAGCCGAATAGTTATACCCTGGCGGCCATGCTTAGTGTAAGTTCAAGTTTGACTATTTTGGAGCATGGGAAACAAATCCATGCCAGTGCCATAAAAGCTGGAGAATCCTCGACACCTTCTGTTACTAATGCCTTGATAGCCATGTATGCTAAAACGGGAAACATCAATGTAGCCAAAAGAGTTTTTGATCTTCCAAATGGGAAGAAGGAAATTGTGTCCTGGACATCAATGATCATGGCTCTCGCACAGCATGGCCTTGGAAAAGAAGCCATCAACCTGTTTGAGAGGATGCTCTCAGTTGGTATGAAACCTGATCATATCACTTATGTAGGAGTGCTCTCTGCTTGTACACATGTGGGATTAGTGGAGCAAGGTCGAAAGTACTATAATATGATGACTGAAGTTCATGAAATTGAACCCACTCTAAGCCATTATGCATGCATGATCGACCTTTATGGACGGGCTGGATTGCTTCAAGAAGCATACCTGTTCATTGAAAGCATGCCTATAGAACCAGATAATATAGCTTGGGGTTCGCTACTAGCATCTTGTAAAATTCATAAAAATGCAGATCTGGCAAAAGTTGCAGCAGAAAGACTGCTTCTTATTGATCCTGGAAATAGTGGCGCCTACTTGGCACTTGCTAATGTGTATTCGGCTTGCGGAAAATGGGAAAATGCTGCCCAAACTAGAAAACTAATGAAGGATAGAGGAGTGAGGAAAGAGAAGGGAATTAGTTGGATTCATATTAAGAATGAAGTTCATGCATTTGGAGTTGAAGACGTTATTCATCCACAGAAAGATGAAATCTACAAGTTGATGGCTGAGATATGGGAGGAGATAAAAAAGATGGGTTTCATCCCAGACACTGAATCAGTACTTCATGACCTTGAAGAAGAGGTGAAAGAGCAAATACTTAAATATCACAGTGAAAAACTTGCTATAGCGTTTGGGCTTTTAAATACTCCAGAGAACACTGCTCTGAGGATTATGAAAAACCTTAGAGTTTGTAATGACTGCCATTCTGCTATAAAGTTCATCTCCAAGCTTGTAGGAAGGGAAATCATTGTGAGAGATGCTACTCGGTTCCACCATTTTAAGGATGGCTCTTGCTCTTGTCGAGACTATTGGTAG

Coding sequence (CDS)

ATGTTGAATGAACCTTCTTTAAAGCCAGATAACTTCACCTTGGCAAGCATTTTATCAGCTTGTGCCAATCTTGAGAAGCTGAATATTGGGAAACAGATACATGCTTATATTCTAAGAGCAGAAACTGAAACTTCTGGAGCAGTTGGCAATGCTTTGATATCAATGTATGCAAAGTCAGGTGGTGTAGAAATAGCTAGACTGATAGTTGAGCATAACAGAACTTCAAATCTAAACATTATAGCGTTCACTTCCCTACTGGATGGATATACCAAGCTAGGAAATGTCAAACCAGCCAGAGAGATATTTAACAAATTAAGAGATCGTGATGTGGTAGCATGGACAGCCATGATTGTTGGTTATGTGCAAAACGGGTTATGGAATGATGCACTGGAGCTGTTTAGGTTGATGGTTAATGAAGGCCCTGAGCCGAATAGTTATACCCTGGCGGCCATGCTTAGTGTAAGTTCAAGTTTGACTATTTTGGAGCATGGGAAACAAATCCATGCCAGTGCCATAAAAGCTGGAGAATCCTCGACACCTTCTGTTACTAATGCCTTGATAGCCATGTATGCTAAAACGGGAAACATCAATGTAGCCAAAAGAGTTTTTGATCTTCCAAATGGGAAGAAGGAAATTGTGTCCTGGACATCAATGATCATGGCTCTCGCACAGCATGGCCTTGGAAAAGAAGCCATCAACCTGTTTGAGAGGATGCTCTCAGTTGGTATGAAACCTGATCATATCACTTATGTAGGAGTGCTCTCTGCTTGTACACATGTGGGATTAGTGGAGCAAGGTCGAAAGTACTATAATATGATGACTGAAGTTCATGAAATTGAACCCACTCTAAGCCATTATGCATGCATGATCGACCTTTATGGACGGGCTGGATTGCTTCAAGAAGCATACCTGTTCATTGAAAGCATGCCTATAGAACCAGATAATATAGCTTGGGGTTCGCTACTAGCATCTTGTAAAATTCATAAAAATGCAGATCTGGCAAAAGTTGCAGCAGAAAGACTGCTTCTTATTGATCCTGGAAATAGTGGCGCCTACTTGGCACTTGCTAATGTGTATTCGGCTTGCGGAAAATGGGAAAATGCTGCCCAAACTAGAAAACTAATGAAGGATAGAGGAGTGAGGAAAGAGAAGGGAATTAGTTGGATTCATATTAAGAATGAAGTTCATGCATTTGGAGTTGAAGACGTTATTCATCCACAGAAAGATGAAATCTACAAGTTGATGGCTGAGATATGGGAGGAGATAAAAAAGATGGGTTTCATCCCAGACACTGAATCAGTACTTCATGACCTTGAAGAAGAGGTGAAAGAGCAAATACTTAAATATCACAGTGAAAAACTTGCTATAGCGTTTGGGCTTTTAAATACTCCAGAGAACACTGCTCTGAGGATTATGAAAAACCTTAGAGTTTGTAATGACTGCCATTCTGCTATAAAGTTCATCTCCAAGCTTGTAGGAAGGGAAATCATTGTGAGAGATGCTACTCGGTTCCACCATTTTAAGGATGGCTCTTGCTCTTGTCGAGACTATTGGTAG

Protein sequence

MLNEPSLKPDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSGGVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGYVQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTPSVTNALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLSVGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQEAYLFIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNSGAYLALANVYSACGKWENAAQTRKLMKDRGVRKEKGISWIHIKNEVHAFGVEDVIHPQKDEIYKLMAEIWEEIKKMGFIPDTESVLHDLEEEVKEQILKYHSEKLAIAFGLLNTPENTALRIMKNLRVCNDCHSAIKFISKLVGREIIVRDATRFHHFKDGSCSCRDYW*
BLAST of Csa5G622560.2 vs. Swiss-Prot
Match: PP168_ARATH (Pentatricopeptide repeat-containing protein At2g22070 OS=Arabidopsis thaliana GN=PCMP-H41 PE=3 SV=1)

HSP 1 Score: 734.6 bits (1895), Expect = 7.7e-211
Identity = 358/518 (69.11%), Postives = 422/518 (81.47%), Query Frame = 1

Query: 1   MLNEPSLKPDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSG 60
           ML +  L PD FTLAS+LSACANLEKL IGKQIH++I+    + SG V NALISMY++ G
Sbjct: 269 MLRDSLLSPDRFTLASVLSACANLEKLCIGKQIHSHIVTTGFDISGIVLNALISMYSRCG 328

Query: 61  GVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGY 120
           GVE AR ++E   T +L I  FT+LLDGY KLG++  A+ IF  L+DRDVVAWTAMIVGY
Sbjct: 329 GVETARRLIEQRGTKDLKIEGFTALLDGYIKLGDMNQAKNIFVSLKDRDVVAWTAMIVGY 388

Query: 121 VQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTP 180
            Q+G + +A+ LFR MV  G  PNSYTLAAMLSV+SSL  L HGKQIH SA+K+GE  + 
Sbjct: 389 EQHGSYGEAINLFRSMVGGGQRPNSYTLAAMLSVASSLASLSHGKQIHGSAVKSGEIYSV 448

Query: 181 SVTNALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLS 240
           SV+NALI MYAK GNI  A R FDL   +++ VSWTSMI+ALAQHG  +EA+ LFE ML 
Sbjct: 449 SVSNALITMYAKAGNITSASRAFDLIRCERDTVSWTSMIIALAQHGHAEEALELFETMLM 508

Query: 241 VGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQ 300
            G++PDHITYVGV SACTH GLV QGR+Y++MM +V +I PTLSHYACM+DL+GRAGLLQ
Sbjct: 509 EGLRPDHITYVGVFSACTHAGLVNQGRQYFDMMKDVDKIIPTLSHYACMVDLFGRAGLLQ 568

Query: 301 EAYLFIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNSGAYLALANVYS 360
           EA  FIE MPIEPD + WGSLL++C++HKN DL KVAAERLLL++P NSGAY ALAN+YS
Sbjct: 569 EAQEFIEKMPIEPDVVTWGSLLSACRVHKNIDLGKVAAERLLLLEPENSGAYSALANLYS 628

Query: 361 ACGKWENAAQTRKLMKDRGVRKEKGISWIHIKNEVHAFGVEDVIHPQKDEIYKLMAEIWE 420
           ACGKWE AA+ RK MKD  V+KE+G SWI +K++VH FGVED  HP+K+EIY  M +IW+
Sbjct: 629 ACGKWEEAAKIRKSMKDGRVKKEQGFSWIEVKHKVHVFGVEDGTHPEKNEIYMTMKKIWD 688

Query: 421 EIKKMGFIPDTESVLHDLEEEVKEQILKYHSEKLAIAFGLLNTPENTALRIMKNLRVCND 480
           EIKKMG++PDT SVLHDLEEEVKEQIL++HSEKLAIAFGL++TP+ T LRIMKNLRVCND
Sbjct: 689 EIKKMGYVPDTASVLHDLEEEVKEQILRHHSEKLAIAFGLISTPDKTTLRIMKNLRVCND 748

Query: 481 CHSAIKFISKLVGREIIVRDATRFHHFKDGSCSCRDYW 519
           CH+AIKFISKLVGREIIVRD TRFHHFKDG CSCRDYW
Sbjct: 749 CHTAIKFISKLVGREIIVRDTTRFHHFKDGFCSCRDYW 786


HSP 2 Score: 117.9 bits (294), Expect = 3.4e-25
Identity = 81/263 (30.80%), Postives = 139/263 (52.85%), Query Frame = 1

Query: 9   PDNFTLASILSACANLEKLNIGKQ--------IHAYILRAETETSGAVGNALISMYAKSG 68
           P   +L+++L  C NL + ++ K         +H  ++++    S  + N L+++Y+K+G
Sbjct: 4   PVPLSLSTLLELCTNLLQKSVNKSNGRFTAQLVHCRVIKSGLMFSVYLMNNLMNVYSKTG 63

Query: 69  GVEIARLIVEHN--RTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIV 128
               AR + +    RT+     ++ ++L  Y+K G++    E F++L  RD V+WT MIV
Sbjct: 64  YALHARKLFDEMPLRTA----FSWNTVLSAYSKRGDMDSTCEFFDQLPQRDSVSWTTMIV 123

Query: 129 GYVQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESS 188
           GY   G ++ A+ +   MV EG EP  +TL  +L+  ++   +E GK++H+  +K G   
Sbjct: 124 GYKNIGQYHKAIRVMGDMVKEGIEPTQFTLTNVLASVAATRCMETGKKVHSFIVKLGLRG 183

Query: 189 TPSVTNALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERM 248
             SV+N+L+ MYAK G+  +AK VFD     ++I SW +MI    Q G    A+  FE+M
Sbjct: 184 NVSVSNSLLNMYAKCGDPMMAKFVFD-RMVVRDISSWNAMIALHMQVGQMDLAMAQFEQM 243

Query: 249 LSVGMKPDHITYVGVLSACTHVG 262
                + D +T+  ++S     G
Sbjct: 244 ----AERDIVTWNSMISGFNQRG 257


HSP 3 Score: 116.3 bits (290), Expect = 9.9e-25
Identity = 68/231 (29.44%), Postives = 133/231 (57.58%), Query Frame = 1

Query: 7   LKPDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSGGVEIAR 66
           ++P  FTL ++L++ A    +  GK++H++I++     + +V N+L++MYAK G   +A+
Sbjct: 142 IEPTQFTLTNVLASVAATRCMETGKKVHSFIVKLGLRGNVSVSNSLLNMYAKCGDPMMAK 201

Query: 67  LIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGYVQNGLW 126
            + +  R    +I ++ +++  + ++G +  A   F ++ +RD+V W +MI G+ Q G  
Sbjct: 202 FVFD--RMVVRDISSWNAMIALHMQVGQMDLAMAQFEQMAERDIVTWNSMISGFNQRGYD 261

Query: 127 NDALELFRLMVNEG-PEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTPSVTNA 186
             AL++F  M+ +    P+ +TLA++LS  ++L  L  GKQIH+  +  G   +  V NA
Sbjct: 262 LRALDIFSKMLRDSLLSPDRFTLASVLSACANLEKLCIGKQIHSHIVTTGFDISGIVLNA 321

Query: 187 LIAMYAKTGNINVAKRVFDLPNGKK-EIVSWTSMIMALAQHGLGKEAINLF 236
           LI+MY++ G +  A+R+ +    K  +I  +T+++    + G   +A N+F
Sbjct: 322 LISMYSRCGGVETARRLIEQRGTKDLKIEGFTALLDGYIKLGDMNQAKNIF 370

BLAST of Csa5G622560.2 vs. Swiss-Prot
Match: PPR57_ARATH (Pentatricopeptide repeat-containing protein At1g25360 OS=Arabidopsis thaliana GN=PCMP-H74 PE=2 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 1.2e-123
Identity = 218/511 (42.66%), Postives = 331/511 (64.77%), Query Frame = 1

Query: 10  DNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSGGVEIARLIV 69
           D FT  S++ ACA    L +GKQ+HAY+LR E + S    N+L+S+Y K G  + AR I 
Sbjct: 285 DEFTYPSVIRACATAGLLQLGKQVHAYVLRRE-DFSFHFDNSLVSLYYKCGKFDEARAIF 344

Query: 70  EHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGYVQNGLWNDA 129
           E     +L  +++ +LL GY   G++  A+ IF +++++++++W  MI G  +NG   + 
Sbjct: 345 EKMPAKDL--VSWNALLSGYVSSGHIGEAKLIFKEMKEKNILSWMIMISGLAENGFGEEG 404

Query: 130 LELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTPSVTNALIAM 189
           L+LF  M  EG EP  Y  +  +   + L    +G+Q HA  +K G  S+ S  NALI M
Sbjct: 405 LKLFSCMKREGFEPCDYAFSGAIKSCAVLGAYCNGQQYHAQLLKIGFDSSLSAGNALITM 464

Query: 190 YAKTGNINVAKRVF-DLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLSVGMKPDHI 249
           YAK G +  A++VF  +P    + VSW ++I AL QHG G EA++++E ML  G++PD I
Sbjct: 465 YAKCGVVEEARQVFRTMPC--LDSVSWNALIAALGQHGHGAEAVDVYEEMLKKGIRPDRI 524

Query: 250 TYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQEAYLFIES 309
           T + VL+AC+H GLV+QGRKY++ M  V+ I P   HYA +IDL  R+G   +A   IES
Sbjct: 525 TLLTVLTACSHAGLVDQGRKYFDSMETVYRIPPGADHYARLIDLLCRSGKFSDAESVIES 584

Query: 310 MPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNSGAYLALANVYSACGKWENA 369
           +P +P    W +LL+ C++H N +L  +AA++L  + P + G Y+ L+N+++A G+WE  
Sbjct: 585 LPFKPTAEIWEALLSGCRVHGNMELGIIAADKLFGLIPEHDGTYMLLSNMHAATGQWEEV 644

Query: 370 AQTRKLMKDRGVRKEKGISWIHIKNEVHAFGVEDVIHPQKDEIYKLMAEIWEEIKKMGFI 429
           A+ RKLM+DRGV+KE   SWI ++ +VH F V+D  HP+ + +Y  + ++ +E++++G++
Sbjct: 645 ARVRKLMRDRGVKKEVACSWIEMETQVHTFLVDDTSHPEAEAVYIYLQDLGKEMRRLGYV 704

Query: 430 PDTESVLHDLEEE-VKEQILKYHSEKLAIAFGLLNTPENTALRIMKNLRVCNDCHSAIKF 489
           PDT  VLHD+E +  KE +L  HSEK+A+AFGL+  P  T +RI KNLR C DCH+  +F
Sbjct: 705 PDTSFVLHDVESDGHKEDMLTTHSEKIAVAFGLMKLPPGTTIRIFKNLRTCGDCHNFFRF 764

Query: 490 ISKLVGREIIVRDATRFHHFKDGSCSCRDYW 519
           +S +V R+II+RD  RFHHF++G CSC ++W
Sbjct: 765 LSWVVQRDIILRDRKRFHHFRNGECSCGNFW 790


HSP 2 Score: 147.1 bits (370), Expect = 5.2e-34
Identity = 111/410 (27.07%), Postives = 193/410 (47.07%), Query Frame = 1

Query: 2   LNEPSLKPDNFTLASILSACANL---EKLNIGKQIHAYILRAETETSGAVGNALISMYAK 61
           +     KPDNFT AS+L+  A +   EK  +  Q HA  L++      +V NAL+S+Y+K
Sbjct: 139 MKHEGFKPDNFTFASVLAGLALVADDEKQCV--QFHAAALKSGAGYITSVSNALVSVYSK 198

Query: 62  SGG----VEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDR-DVVAW 121
                  +  AR + +     +    ++T+++ GY K G      E+   + D   +VA+
Sbjct: 199 CASSPSLLHSARKVFDEILEKDER--SWTTMMTGYVKNGYFDLGEELLEGMDDNMKLVAY 258

Query: 122 TAMIVGYVQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIK 181
            AMI GYV  G + +ALE+ R MV+ G E + +T  +++   ++  +L+ GKQ+HA  ++
Sbjct: 259 NAMISGYVNRGFYQEALEMVRRMVSSGIELDEFTYPSVIRACATAGLLQLGKQVHAYVLR 318

Query: 182 AGESSTPSVTNALIAMYAKTGNINVAKRVFDLPNGK------------------------ 241
             + S     N+L+++Y K G  + A+ +F+    K                        
Sbjct: 319 REDFSF-HFDNSLVSLYYKCGKFDEARAIFEKMPAKDLVSWNALLSGYVSSGHIGEAKLI 378

Query: 242 ------KEIVSWTSMIMALAQHGLGKEAINLFERMLSVGMKPDHITYVGVLSACTHVGLV 301
                 K I+SW  MI  LA++G G+E + LF  M   G +P    + G + +C  +G  
Sbjct: 379 FKEMKEKNILSWMIMISGLAENGFGEEGLKLFSCMKREGFEPCDYAFSGAIKSCAVLGAY 438

Query: 302 EQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQEAYLFIESMPIEPDNIAWGSLLA 361
             G++Y+  + ++   + +LS    +I +Y + G+++EA     +MP   D+++W +L+A
Sbjct: 439 CNGQQYHAQLLKI-GFDSSLSAGNALITMYAKCGVVEEARQVFRTMPC-LDSVSWNALIA 498

Query: 362 SCKIHKNADLAKVAAERLLLIDPGNSGAYLALANVYSACGKWENAAQTRK 374
           +   H +   A    E +L    G     + L  V +AC       Q RK
Sbjct: 499 ALGQHGHGAEAVDVYEEML--KKGIRPDRITLLTVLTACSHAGLVDQGRK 539


HSP 3 Score: 97.8 bits (242), Expect = 3.6e-19
Identity = 85/298 (28.52%), Postives = 146/298 (48.99%), Query Frame = 1

Query: 15  ASILSACANLEK--LNIGKQIHAYILRAETETSGAVGNALISMYAKSGGVEIARLIVEHN 74
           A+ L  C  L +  L + + +H  I+    +    + N LI +Y KS  +  AR + +  
Sbjct: 16  AANLRLCLPLRRTSLQLARAVHGNIITFGFQPRAHILNRLIDVYCKSSELNYARQLFDE- 75

Query: 75  RTSNLNIIAFTSLLDGYTKLGNVKPAREIFNK--LRDRDVVAWTAMIVGYVQNGLWNDAL 134
             S  + IA T+++ GY   G++  AR +F K  +  RD V + AMI G+  N     A+
Sbjct: 76  -ISEPDKIARTTMVSGYCASGDITLARGVFEKAPVCMRDTVMYNAMITGFSHNNDGYSAI 135

Query: 135 ELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHG-KQIHASAIKAGESSTPSVTNALIAM 194
            LF  M +EG +P+++T A++L+  + +   E    Q HA+A+K+G     SV+NAL+++
Sbjct: 136 NLFCKMKHEGFKPDNFTFASVLAGLALVADDEKQCVQFHAAALKSGAGYITSVSNALVSV 195

Query: 195 YAKTGN----INVAKRVFDLPNGKKEIVSWTSMIMALAQHG---LGKEAINLFERMLSVG 254
           Y+K  +    ++ A++VFD    K E  SWT+M+    ++G   LG+E +   +  + + 
Sbjct: 196 YSKCASSPSLLHSARKVFDEILEKDE-RSWTTMMTGYVKNGYFDLGEELLEGMDDNMKL- 255

Query: 255 MKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQ 301
                + Y  ++S   + G  ++  +    M     IE     Y  +I     AGLLQ
Sbjct: 256 -----VAYNAMISGYVNRGFYQEALEMVRRMVS-SGIELDEFTYPSVIRACATAGLLQ 303


HSP 4 Score: 61.2 bits (147), Expect = 3.8e-08
Identity = 81/356 (22.75%), Postives = 149/356 (41.85%), Query Frame = 1

Query: 98  AREIFNKLRDRDVVAWTAMIVGYVQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSS 157
           AR++F+++ ++D  +WT M+ GYV+NG ++   EL      EG + N   +A    +S  
Sbjct: 207 ARKVFDEILEKDERSWTTMMTGYVKNGYFDLGEELL-----EGMDDNMKLVAYNAMISGY 266

Query: 158 LT------ILEHGKQIHASAIKAGESSTPSVTNALIAMYAKTGNINVAKRVFDLPNGKKE 217
           +        LE  +++ +S I+  E + PSV  A     A  G + + K+V      +++
Sbjct: 267 VNRGFYQEALEMVRRMVSSGIELDEFTYPSVIRAC----ATAGLLQLGKQVHAYVLRRED 326

Query: 218 IVSW--TSMIMALAQHGLGKEAINLFERMLSVGMKPDHITYVGVLSACTHVGLVEQGRKY 277
                  S++    + G   EA  +FE+M +     D +++  +LS     G + + +  
Sbjct: 327 FSFHFDNSLVSLYYKCGKFDEARAIFEKMPA----KDLVSWNALLSGYVSSGHIGEAKLI 386

Query: 278 YNMMTEVHEIEPTLSHYACMIDLYGRAGLLQEAYLFIESMPIEPDNIAWGSLLASCKI-- 337
           +  M E + +   +       + +G  GL  + +  ++    EP + A+   + SC +  
Sbjct: 387 FKEMKEKNILSWMIMISGLAENGFGEEGL--KLFSCMKREGFEPCDYAFSGAIKSCAVLG 446

Query: 338 ------HKNADLAKVAAERLLLIDPGNSGAYLALANVYSACGKWENAAQTRKLMKDRGVR 397
                   +A L K+  +  L    GN     AL  +Y+ CG  E A Q  + M      
Sbjct: 447 AYCNGQQYHAQLLKIGFDSSL--SAGN-----ALITMYAKCGVVEEARQVFRTMPCL--- 506

Query: 398 KEKGISWIHIKNEV--HAFGVEDVIHPQKDEIYKLMAEIWEEIKKMGFIPDTESVL 436
               +SW  +   +  H  G E V             +++EE+ K G  PD  ++L
Sbjct: 507 --DSVSWNALIAALGQHGHGAEAV-------------DVYEEMLKKGIRPDRITLL 522

BLAST of Csa5G622560.2 vs. Swiss-Prot
Match: PPR53_ARATH (Pentatricopeptide repeat-containing protein At1g20230 OS=Arabidopsis thaliana GN=PCMP-H21 PE=2 SV=2)

HSP 1 Score: 444.5 bits (1142), Expect = 1.6e-123
Identity = 221/514 (43.00%), Postives = 323/514 (62.84%), Query Frame = 1

Query: 9   PDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSGGVEIARLI 68
           PD  T++S+L +  + E LN+G+ IH Y+++        V +A+I MY KSG   +  +I
Sbjct: 250 PDQVTVSSVLPSVGDSEMLNMGRLIHGYVIKQGLLKDKCVISAMIDMYGKSG--HVYGII 309

Query: 69  VEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDR----DVVAWTAMIVGYVQNG 128
              N+   +      + + G ++ G V  A E+F   +++    +VV+WT++I G  QNG
Sbjct: 310 SLFNQFEMMEAGVCNAYITGLSRNGLVDKALEMFELFKEQTMELNVVSWTSIIAGCAQNG 369

Query: 129 LWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTPSVTN 188
              +ALELFR M   G +PN  T+ +ML    ++  L HG+  H  A++        V +
Sbjct: 370 KDIEALELFREMQVAGVKPNHVTIPSMLPACGNIAALGHGRSTHGFAVRVHLLDNVHVGS 429

Query: 189 ALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLSVGMK 248
           ALI MYAK G IN+++ VF++    K +V W S++   + HG  KE +++FE ++   +K
Sbjct: 430 ALIDMYAKCGRINLSQIVFNMMP-TKNLVCWNSLMNGFSMHGKAKEVMSIFESLMRTRLK 489

Query: 249 PDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQEAYL 308
           PD I++  +LSAC  VGL ++G KY+ MM+E + I+P L HY+CM++L GRAG LQEAY 
Sbjct: 490 PDFISFTSLLSACGQVGLTDEGWKYFKMMSEEYGIKPRLEHYSCMVNLLGRAGKLQEAYD 549

Query: 309 FIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNSGAYLALANVYSACGK 368
            I+ MP EPD+  WG+LL SC++  N DLA++AAE+L  ++P N G Y+ L+N+Y+A G 
Sbjct: 550 LIKEMPFEPDSCVWGALLNSCRLQNNVDLAEIAAEKLFHLEPENPGTYVLLSNIYAAKGM 609

Query: 369 WENAAQTRKLMKDRGVRKEKGISWIHIKNEVHAFGVEDVIHPQKDEIYKLMAEIWEEIKK 428
           W      R  M+  G++K  G SWI +KN V+     D  HPQ D+I + M EI +E++K
Sbjct: 610 WTEVDSIRNKMESLGLKKNPGCSWIQVKNRVYTLLAGDKSHPQIDQITEKMDEISKEMRK 669

Query: 429 MGFIPDTESVLHDLEEEVKEQILKYHSEKLAIAFGLLNTPENTALRIMKNLRVCNDCHSA 488
            G  P+ +  LHD+EE+ +EQ+L  HSEKLA+ FGLLNTP+ T L+++KNLR+C DCH+ 
Sbjct: 670 SGHRPNLDFALHDVEEQEQEQMLWGHSEKLAVVFGLLNTPDGTPLQVIKNLRICGDCHAV 729

Query: 489 IKFISKLVGREIIVRDATRFHHFKDGSCSCRDYW 519
           IKFIS   GREI +RD  RFHHFKDG CSC D+W
Sbjct: 730 IKFISSYAGREIFIRDTNRFHHFKDGICSCGDFW 760


HSP 2 Score: 105.1 bits (261), Expect = 2.3e-21
Identity = 104/476 (21.85%), Postives = 194/476 (40.76%), Query Frame = 1

Query: 7   LKPDNFTLASILSACANLEKLNIGK--------------------QIHAY---------- 66
           L PD+  L ++   CA L    +GK                      H Y          
Sbjct: 112 LIPDSHVLPNLFKVCAELSAFKVGKQIHCVSCVSGLDMDAFVQGSMFHMYMRCGRMGDAR 171

Query: 67  -ILRAETETSGAVGNALISMYAKSGGVEIARLIVEHNRTSNL--NIIAFTSLLDGYTKLG 126
            +    ++      +AL+  YA+ G +E    I+    +S +  NI+++  +L G+ + G
Sbjct: 172 KVFDRMSDKDVVTCSALLCAYARKGCLEEVVRILSEMESSGIEANIVSWNGILSGFNRSG 231

Query: 127 NVKPAREIFNKLRDR----DVVAWTAMIVGYVQNGLWNDALELFRLMVNEGPEPNSYTLA 186
             K A  +F K+       D V  ++++     + + N    +   ++ +G   +   ++
Sbjct: 232 YHKEAVVMFQKIHHLGFCPDQVTVSSVLPSVGDSEMLNMGRLIHGYVIKQGLLKDKCVIS 291

Query: 187 AMLSVSSSLTILEHGKQIHA----SAIKAGESSTPSVTNALIAMYAKTGNINVAKRVFDL 246
           AM+ +        +GK  H     S     E     V NA I   ++ G ++ A  +F+L
Sbjct: 292 AMIDM--------YGKSGHVYGIISLFNQFEMMEAGVCNAYITGLSRNGLVDKALEMFEL 351

Query: 247 ---PNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLSVGMKPDHITYVGVLSACTHVGL 306
                 +  +VSWTS+I   AQ+G   EA+ LF  M   G+KP+H+T   +L AC ++  
Sbjct: 352 FKEQTMELNVVSWTSIIAGCAQNGKDIEALELFREMQVAGVKPNHVTIPSMLPACGNIAA 411

Query: 307 VEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQEAYLFIESMPIEPDNIAWGSLL 366
           +  GR  +     VH ++  +   + +ID+Y + G +  + +    MP + + + W SL+
Sbjct: 412 LGHGRSTHGFAVRVHLLD-NVHVGSALIDMYAKCGRINLSQIVFNMMPTK-NLVCWNSLM 471

Query: 367 ASCKIHKNADLAKVAAERLL--LIDPGNSGAYLALANVYSACGKWENAAQTRKLMKDRGV 426
               +H  A       E L+   + P     +++  ++ SACG+          + D G 
Sbjct: 472 NGFSMHGKAKEVMSIFESLMRTRLKPD----FISFTSLLSACGQVG--------LTDEG- 531

Query: 427 RKEKGISWIHIKNEVHAFGVEDVIHPQKDEIYKL-----MAEIWEEIKKMGFIPDT 432
                  W + K     +G++  +      +  L     + E ++ IK+M F PD+
Sbjct: 532 -------WKYFKMMSEEYGIKPRLEHYSCMVNLLGRAGKLQEAYDLIKEMPFEPDS 557

BLAST of Csa5G622560.2 vs. Swiss-Prot
Match: PP301_ARATH (Pentatricopeptide repeat-containing protein At4g02750 OS=Arabidopsis thaliana GN=PCMP-H24 PE=3 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 1.9e-121
Identity = 205/469 (43.71%), Postives = 311/469 (66.31%), Query Frame = 1

Query: 50  NALISMYAKSGGVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRD 109
           NA+++ Y +   +E+A+ + +     N++   + +++ GY + G +  A+ +F+K+  RD
Sbjct: 316 NAMLAGYVQGERMEMAKELFDVMPCRNVS--TWNTMITGYAQCGKISEAKNLFDKMPKRD 375

Query: 110 VVAWTAMIVGYVQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHA 169
            V+W AMI GY Q+G   +AL LF  M  EG   N  + ++ LS  + +  LE GKQ+H 
Sbjct: 376 PVSWAAMIAGYSQSGHSFEALRLFVQMEREGGRLNRSSFSSALSTCADVVALELGKQLHG 435

Query: 170 SAIKAGESSTPSVTNALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGK 229
             +K G  +   V NAL+ MY K G+I  A  +F    GK +IVSW +MI   ++HG G+
Sbjct: 436 RLVKGGYETGCFVGNALLLMYCKCGSIEEANDLFKEMAGK-DIVSWNTMIAGYSRHGFGE 495

Query: 230 EAINLFERMLSVGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACM 289
            A+  FE M   G+KPD  T V VLSAC+H GLV++GR+Y+  MT+ + + P   HYACM
Sbjct: 496 VALRFFESMKREGLKPDDATMVAVLSACSHTGLVDKGRQYFYTMTQDYGVMPNSQHYACM 555

Query: 290 IDLYGRAGLLQEAYLFIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNS 349
           +DL GRAGLL++A+  +++MP EPD   WG+LL + ++H N +LA+ AA+++  ++P NS
Sbjct: 556 VDLLGRAGLLEDAHNLMKNMPFEPDAAIWGTLLGASRVHGNTELAETAADKIFAMEPENS 615

Query: 350 GAYLALANVYSACGKWENAAQTRKLMKDRGVRKEKGISWIHIKNEVHAFGVEDVIHPQKD 409
           G Y+ L+N+Y++ G+W +  + R  M+D+GV+K  G SWI I+N+ H F V D  HP+KD
Sbjct: 616 GMYVLLSNLYASSGRWGDVGKLRVRMRDKGVKKVPGYSWIEIQNKTHTFSVGDEFHPEKD 675

Query: 410 EIYKLMAEIWEEIKKMGFIPDTESVLHDLEEEVKEQILKYHSEKLAIAFGLLNTPENTAL 469
           EI+  + E+   +KK G++  T  VLHD+EEE KE++++YHSE+LA+A+G++       +
Sbjct: 676 EIFAFLEELDLRMKKAGYVSKTSVVLHDVEEEEKERMVRYHSERLAVAYGIMRVSSGRPI 735

Query: 470 RIMKNLRVCNDCHSAIKFISKLVGREIIVRDATRFHHFKDGSCSCRDYW 519
           R++KNLRVC DCH+AIK+++++ GR II+RD  RFHHFKDGSCSC DYW
Sbjct: 736 RVIKNLRVCEDCHNAIKYMARITGRLIILRDNNRFHHFKDGSCSCGDYW 781


HSP 2 Score: 105.9 bits (263), Expect = 1.3e-21
Identity = 81/289 (28.03%), Postives = 144/289 (49.83%), Query Frame = 1

Query: 51  ALISMYAKSGGVEIARLIVEHNR---TSNL-NIIAFTSLLDGYTKLGNVKPAREIFNKLR 110
           AL+S     GG    + IVE  +   + N+ +++++ +++ GY + G +  AR++F++  
Sbjct: 218 ALVSWNCLLGGFVKKKKIVEARQFFDSMNVRDVVSWNTIITGYAQSGKIDEARQLFDESP 277

Query: 111 DRDVVAWTAMIVGYVQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQ 170
            +DV  WTAM+ GY+QN +  +A ELF  M    PE N  +  AML+       +E  K+
Sbjct: 278 VQDVFTWTAMVSGYIQNRMVEEARELFDKM----PERNEVSWNAMLAGYVQGERMEMAKE 337

Query: 171 IHASAIKAGESSTPSVTNALIAMYAKTGNINVAKRVFD-LPNGKKEIVSWTSMIMALAQH 230
           +             S  N +I  YA+ G I+ AK +FD +P  K++ VSW +MI   +Q 
Sbjct: 338 L----FDVMPCRNVSTWNTMITGYAQCGKISEAKNLFDKMP--KRDPVSWAAMIAGYSQS 397

Query: 231 GLGKEAINLFERMLSVGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSH 290
           G   EA+ LF +M   G + +  ++   LS C  V  +E G++ +  + +          
Sbjct: 398 GHSFEALRLFVQMEREGGRLNRSSFSSALSTCADVVALELGKQLHGRLVKGGYETGCFVG 457

Query: 291 YACMIDLYGRAGLLQEAYLFIESMPIEPDNIAWGSLLASCKIHKNADLA 335
            A ++ +Y + G ++EA    + M    D ++W +++A    H   ++A
Sbjct: 458 NALLL-MYCKCGSIEEANDLFKEM-AGKDIVSWNTMIAGYSRHGFGEVA 494


HSP 3 Score: 98.2 bits (243), Expect = 2.8e-19
Identity = 80/300 (26.67%), Postives = 136/300 (45.33%), Query Frame = 1

Query: 50  NALISMYAKSGGVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRD 109
           N +IS Y ++G  E+AR + +     +L  +++  ++ GY +  N+  ARE+F  + +RD
Sbjct: 99  NGMISGYLRNGEFELARKLFDEMPERDL--VSWNVMIKGYVRNRNLGKARELFEIMPERD 158

Query: 110 VVAWTAMIVGYVQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHA 169
           V +W  M+ GY QNG  +DA  +F  M    PE N  +  A+LS     + +E    +  
Sbjct: 159 VCSWNTMLSGYAQNGCVDDARSVFDRM----PEKNDVSWNALLSAYVQNSKMEEACML-- 218

Query: 170 SAIKAGESSTPSVTNALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGK 229
              K+ E+      N L+  + K   I  A++ FD  N  +++VSW ++I   AQ G   
Sbjct: 219 --FKSRENWALVSWNCLLGGFVKKKKIVEARQFFDSMN-VRDVVSWNTIITGYAQSGKID 278

Query: 230 EAINLFERMLSVGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEI--EPTLSHYA 289
           EA  LF+         D  T+  ++S      +VE+ R+ ++ M E +E+     L+ Y 
Sbjct: 279 EARQLFDE----SPVQDVFTWTAMVSGYIQNRMVEEARELFDKMPERNEVSWNAMLAGYV 338

Query: 290 ------------------------CMIDLYGRAGLLQEAYLFIESMPIEPDNIAWGSLLA 324
                                    MI  Y + G + EA    + MP + D ++W +++A
Sbjct: 339 QGERMEMAKELFDVMPCRNVSTWNTMITGYAQCGKISEAKNLFDKMP-KRDPVSWAAMIA 382


HSP 4 Score: 79.0 bits (193), Expect = 1.8e-13
Identity = 74/339 (21.83%), Postives = 153/339 (45.13%), Query Frame = 1

Query: 50  NALISMYAKSGGVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRD 109
           N  IS Y ++G    A  + +  R    + +++  ++ GY + G  + AR++F+++ +RD
Sbjct: 68  NVAISSYMRTGRCNEALRVFK--RMPRWSSVSYNGMISGYLRNGEFELARKLFDEMPERD 127

Query: 110 VVAWTAMIVGYVQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHA 169
           +V+W  MI GYV+N     A ELF +M    PE +  +   MLS  +    ++  + +  
Sbjct: 128 LVSWNVMIKGYVRNRNLGKARELFEIM----PERDVCSWNTMLSGYAQNGCVDDARSVFD 187

Query: 170 SAIKAGESSTPSVTNALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGK 229
              +  + S     NAL++ Y +   +  A  +F        +VSW  ++    +     
Sbjct: 188 RMPEKNDVS----WNALLSAYVQNSKMEEACMLFK-SRENWALVSWNCLLGGFVKKKKIV 247

Query: 230 EAINLFERMLSVGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACM 289
           EA   F+ M       D +++  +++     G +++ R+ ++  + V ++      +  M
Sbjct: 248 EARQFFDSM----NVRDVVSWNTIITGYAQSGKIDEARQLFD-ESPVQDV----FTWTAM 307

Query: 290 IDLYGRAGLLQEAYLFIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNS 349
           +  Y +  +++EA    + MP E + ++W ++LA    +   +  ++A E   ++   N 
Sbjct: 308 VSGYIQNRMVEEARELFDKMP-ERNEVSWNAMLAG---YVQGERMEMAKELFDVMPCRNV 367

Query: 350 GAYLALANVYSACGKWENAAQTRKLMKDRGVRKEKGISW 389
             +  +   Y+ CGK   A    K + D+ + K   +SW
Sbjct: 368 STWNTMITGYAQCGKISEA----KNLFDK-MPKRDPVSW 377


HSP 5 Score: 78.6 bits (192), Expect = 2.3e-13
Identity = 91/395 (23.04%), Postives = 167/395 (42.28%), Query Frame = 1

Query: 50  NALISMYAKSGGVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRD 109
           N +I+ YA+SG ++ AR + + +   +  +  +T+++ GY +   V+ ARE+F+K+ +R+
Sbjct: 254 NTIITGYAQSGKIDEARQLFDESPVQD--VFTWTAMVSGYIQNRMVEEARELFDKMPERN 313

Query: 110 VVAWTAMIVGYVQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHA 169
            V+W AM+ GYVQ      A ELF +M    P  N  T   M++  +    +   K +  
Sbjct: 314 EVSWNAMLAGYVQGERMEMAKELFDVM----PCRNVSTWNTMITGYAQCGKISEAKNLFD 373

Query: 170 SAIKAGESSTPSVTNALIAMYAKTGNINVAKRVF---DLPNGKKEIVSWTSMIMALAQHG 229
              K      P    A+IA Y+++G+   A R+F   +   G+    S++S +   A   
Sbjct: 374 KMPK----RDPVSWAAMIAGYSQSGHSFEALRLFVQMEREGGRLNRSSFSSALSTCADVV 433

Query: 230 LGKEAINLFERMLSVGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHY 289
             +    L  R++  G +        +L      G +E+    +  M         +  +
Sbjct: 434 ALELGKQLHGRLVKGGYETGCFVGNALLLMYCKCGSIEEANDLFKEMA-----GKDIVSW 493

Query: 290 ACMIDLYGRAGLLQEAYLFIESMP---IEPDNIAWGSLLASCKIHKNADLAKVAAERLLL 349
             MI  Y R G  + A  F ESM    ++PD+    ++L++C      D  +     +  
Sbjct: 494 NTMIAGYSRHGFGEVALRFFESMKREGLKPDDATMVAVLSACSHTGLVDKGRQYFYTMTQ 553

Query: 350 IDPG---NSGAYLALANVYSACGKWENAAQTRKLMKDRGVRKEK-------GISWIHIKN 409
            D G   NS  Y  + ++    G  E+A     LMK+     +        G S +H   
Sbjct: 554 -DYGVMPNSQHYACMVDLLGRAGLLEDA---HNLMKNMPFEPDAAIWGTLLGASRVHGNT 613

Query: 410 EVHAFGVEDV--IHPQKDEIYKLMAEIWEEIKKMG 427
           E+     + +  + P+   +Y L++ ++    + G
Sbjct: 614 ELAETAADKIFAMEPENSGMYVLLSNLYASSGRWG 629


HSP 6 Score: 65.5 bits (158), Expect = 2.0e-09
Identity = 50/215 (23.26%), Postives = 89/215 (41.40%), Query Frame = 1

Query: 13  TLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSGGVEIARLIVEHN 72
           + +S LS CA++  L +GKQ+H  +++   ET   VGNAL+ MY K G +E A  + +  
Sbjct: 411 SFSSALSTCADVVALELGKQLHGRLVKGGYETGCFVGNALLLMYCKCGSIEEANDLFK-- 470

Query: 73  RTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGYVQNGLWNDALEL 132
             +  +I+++ +                               MI GY ++G    AL  
Sbjct: 471 EMAGKDIVSWNT-------------------------------MIAGYSRHGFGEVALRF 530

Query: 133 FRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQ-IHASAIKAGESSTPSVTNALIAMYA 192
           F  M  EG +P+  T+ A+LS  S   +++ G+Q  +      G          ++ +  
Sbjct: 531 FESMKREGLKPDDATMVAVLSACSHTGLVDKGRQYFYTMTQDYGVMPNSQHYACMVDLLG 590

Query: 193 KTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHG 227
           + G +  A  +      + +   W +++ A   HG
Sbjct: 591 RAGLLEDAHNLMKNMPFEPDAAIWGTLLGASRVHG 592

BLAST of Csa5G622560.2 vs. Swiss-Prot
Match: PP425_ARATH (Pentatricopeptide repeat-containing protein At5g48910 OS=Arabidopsis thaliana GN=PCMP-H38 PE=2 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 2.1e-120
Identity = 224/531 (42.18%), Postives = 330/531 (62.15%), Query Frame = 1

Query: 1   MLNEPSLKPDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSG 60
           M+++  ++P+ FT  S+L ACA   K+  GKQIH   L+        V + L+ MY   G
Sbjct: 118 MMSDEFVEPNRFTFPSVLKACAKTGKIQEGKQIHGLALKYGFGGDEFVMSNLVRMYVMCG 177

Query: 61  GVEIARLIVEHN------------RTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDR 120
            ++ AR++   N            R  +  I+ +  ++DGY +LG+ K AR +F+K+R R
Sbjct: 178 FMKDARVLFYKNIIEKDMVVMTDRRKRDGEIVLWNVMIDGYMRLGDCKAARMLFDKMRQR 237

Query: 121 DVVAWTAMIVGYVQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIH 180
            VV+W  MI GY  NG + DA+E+FR M      PN  TL ++L   S L  LE G+ +H
Sbjct: 238 SVVSWNTMISGYSLNGFFKDAVEVFREMKKGDIRPNYVTLVSVLPAISRLGSLELGEWLH 297

Query: 181 ASAIKAGESSTPSVTNALIAMYAKTGNINVAKRVFD-LPNGKKEIVSWTSMIMALAQHGL 240
             A  +G      + +ALI MY+K G I  A  VF+ LP  ++ +++W++MI   A HG 
Sbjct: 298 LYAEDSGIRIDDVLGSALIDMYSKCGIIEKAIHVFERLP--RENVITWSAMINGFAIHGQ 357

Query: 241 GKEAINLFERMLSVGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYA 300
             +AI+ F +M   G++P  + Y+ +L+AC+H GLVE+GR+Y++ M  V  +EP + HY 
Sbjct: 358 AGDAIDCFCKMRQAGVRPSDVAYINLLTACSHGGLVEEGRRYFSQMVSVDGLEPRIEHYG 417

Query: 301 CMIDLYGRAGLLQEAYLFIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPG 360
           CM+DL GR+GLL EA  FI +MPI+PD++ W +LL +C++  N ++ K  A  L+ + P 
Sbjct: 418 CMVDLLGRSGLLDEAEEFILNMPIKPDDVIWKALLGACRMQGNVEMGKRVANILMDMVPH 477

Query: 361 NSGAYLALANVYSACGKWENAAQTRKLMKDRGVRKEKGISWIHIKNEVHAFGVEDVIHPQ 420
           +SGAY+AL+N+Y++ G W   ++ R  MK++ +RK+ G S I I   +H F VED  HP+
Sbjct: 478 DSGAYVALSNMYASQGNWSEVSEMRLRMKEKDIRKDPGCSLIDIDGVLHEFVVEDDSHPK 537

Query: 421 KDEIYKLMAEIWEEIKKMGFIPDTESVLHDLEEEVKEQILKYHSEKLAIAFGLLNTPENT 480
             EI  ++ EI ++++  G+ P T  VL +LEEE KE +L YHSEK+A AFGL++T    
Sbjct: 538 AKEINSMLVEISDKLRLAGYRPITTQVLLNLEEEDKENVLHYHSEKIATAFGLISTSPGK 597

Query: 481 ALRIMKNLRVCNDCHSAIKFISKLVGREIIVRDATRFHHFKDGSCSCRDYW 519
            +RI+KNLR+C DCHS+IK ISK+  R+I VRD  RFHHF+DGSCSC DYW
Sbjct: 598 PIRIVKNLRICEDCHSSIKLISKVYKRKITVRDRKRFHHFQDGSCSCMDYW 646

BLAST of Csa5G622560.2 vs. TrEMBL
Match: B9HFU9_POPTR (Pentatricopeptide repeat-containing family protein OS=Populus trichocarpa GN=POPTR_0007s06200g PE=4 SV=2)

HSP 1 Score: 811.6 bits (2095), Expect = 5.5e-232
Identity = 389/518 (75.10%), Postives = 462/518 (89.19%), Query Frame = 1

Query: 1   MLNEPSLKPDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSG 60
           +L + SLKPD F+LAS LSACANLEKL+ GKQIH YI+R   + SGAVGNALISMYAKSG
Sbjct: 268 ILKDTSLKPDRFSLASALSACANLEKLSFGKQIHGYIVRTMFDASGAVGNALISMYAKSG 327

Query: 61  GVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGY 120
           GVEIAR I+E +  S+L++IAFT+LL+GY KLG++ PAR+IFN L+D DVVAWTAMIVGY
Sbjct: 328 GVEIARRIIEQSGISDLDVIAFTALLNGYVKLGDITPARQIFNSLKDPDVVAWTAMIVGY 387

Query: 121 VQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTP 180
           VQNGL NDA+E+F+ MV+EGP PNS+TLAAMLS SSS+T L HGKQIHASAI++GE+ +P
Sbjct: 388 VQNGLNNDAIEVFKTMVSEGPRPNSFTLAAMLSASSSVTSLNHGKQIHASAIRSGEALSP 447

Query: 181 SVTNALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLS 240
           SV NAL  MYAK G+IN A++VF+L    ++ VSWTSMIMALAQHGLG+EAI LFE+ML+
Sbjct: 448 SVGNALTTMYAKAGSINGARKVFNLLRQNRDTVSWTSMIMALAQHGLGEEAIELFEQMLT 507

Query: 241 VGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQ 300
           +G+KPDHITYVGVLSACTH GLVEQGR Y+++M  VH+I+PTLSHYACM+DL+GRAGLLQ
Sbjct: 508 LGIKPDHITYVGVLSACTHGGLVEQGRSYFDLMKNVHKIDPTLSHYACMVDLFGRAGLLQ 567

Query: 301 EAYLFIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNSGAYLALANVYS 360
           EAY F+E+MP+EPD IAWGSLL+SCK++KN DLAKVAAERLLLI+P NSGAY ALANVYS
Sbjct: 568 EAYKFVENMPMEPDVIAWGSLLSSCKVYKNVDLAKVAAERLLLIEPNNSGAYSALANVYS 627

Query: 361 ACGKWENAAQTRKLMKDRGVRKEKGISWIHIKNEVHAFGVEDVIHPQKDEIYKLMAEIWE 420
           +CGKW++AA+ RKLMK RGV+KE+G+SW+ I+N+ H FGVED +HPQKDEIYK+M +IW+
Sbjct: 628 SCGKWDDAAKIRKLMKARGVKKEQGLSWVQIQNKTHVFGVEDGLHPQKDEIYKMMDKIWK 687

Query: 421 EIKKMGFIPDTESVLHDLEEEVKEQILKYHSEKLAIAFGLLNTPENTALRIMKNLRVCND 480
           EIKKMGF PDTESVLHDLE EVK+QIL+YHSEKLAIAFG+++TPENT LRIMKNLRVCND
Sbjct: 688 EIKKMGFAPDTESVLHDLEVEVKDQILRYHSEKLAIAFGIISTPENTTLRIMKNLRVCND 747

Query: 481 CHSAIKFISKLVGREIIVRDATRFHHFKDGSCSCRDYW 519
           CH+AIKFISKLV REIIVRDATRFHHFKDGSCSC+DYW
Sbjct: 748 CHNAIKFISKLVDREIIVRDATRFHHFKDGSCSCKDYW 785

BLAST of Csa5G622560.2 vs. TrEMBL
Match: B9HFU9_POPTR (Pentatricopeptide repeat-containing family protein OS=Populus trichocarpa GN=POPTR_0007s06200g PE=4 SV=2)

HSP 1 Score: 130.6 bits (327), Expect = 5.6e-27
Identity = 73/260 (28.08%), Postives = 148/260 (56.92%), Query Frame = 1

Query: 9   PDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSGGVEIARLI 68
           P  FTL ++L++CA      IGK++H+++++        V N+L++MYAK+G +++A+++
Sbjct: 143 PTQFTLTNVLASCAATGSRGIGKKVHSFVVKLGLHACVPVANSLLNMYAKTGDLKMAKVV 202

Query: 69  VEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGYVQNGLWND 128
            +  +  N +  ++ +++  +   G V  A   F  L +RD+V+W +MI G  Q+G  N+
Sbjct: 203 FDRMKLRNTS--SWNAMISLHMNCGRVDLALAQFELLSERDIVSWNSMIAGCNQHGFDNE 262

Query: 129 ALELF-RLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTPSVTNALI 188
           AL+ F  ++ +   +P+ ++LA+ LS  ++L  L  GKQIH   ++    ++ +V NALI
Sbjct: 263 ALQFFSSILKDTSLKPDRFSLASALSACANLEKLSFGKQIHGYIVRTMFDASGAVGNALI 322

Query: 189 AMYAKTGNINVAKRVFDLPN-GKKEIVSWTSMIMALAQHGLGKEAINLFERMLSVGMKPD 248
           +MYAK+G + +A+R+ +       +++++T+++    + G    A  +F  +      PD
Sbjct: 323 SMYAKSGGVEIARRIIEQSGISDLDVIAFTALLNGYVKLGDITPARQIFNSL----KDPD 382

Query: 249 HITYVGVLSACTHVGLVEQG 267
            + +  ++     VG V+ G
Sbjct: 383 VVAWTAMI-----VGYVQNG 391


HSP 2 Score: 113.2 bits (282), Expect = 9.3e-22
Identity = 93/371 (25.07%), Postives = 176/371 (47.44%), Query Frame = 1

Query: 20  ACANLEKLNI-------GKQIHAYILRAETETSGAVGNALISMYAKSGGVEIARLIVEHN 79
           +CA+L + ++       GK IHA +++     S  + N L+++YAK+G    A  +   N
Sbjct: 15  SCAHLLQTSLKSRDPLKGKSIHAQMVKLGLTFSVYLMNNLMNLYAKTGFHLDAHDLF--N 74

Query: 80  RTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGYVQNGLWNDALEL 139
                   ++ ++L GY K G ++ A ++F+ +  RD V+WT +IVGY Q G + DA+++
Sbjct: 75  EMPVKTTFSWNTILSGYAKQGKLEKAHQVFDLIPVRDSVSWTTIIVGYNQMGRFEDAIKI 134

Query: 140 FRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTPSVTNALIAMYAK 199
           F  MV +   P  +TL  +L+  ++      GK++H+  +K G  +   V N+L+ MYAK
Sbjct: 135 FVDMVKDKVLPTQFTLTNVLASCAATGSRGIGKKVHSFVVKLGLHACVPVANSLLNMYAK 194

Query: 200 TGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLSVGMKPDHITYVG 259
           TG++ +AK VFD     +   SW +MI      G    A+  FE +     + D +++  
Sbjct: 195 TGDLKMAKVVFDRMK-LRNTSSWNAMISLHMNCGRVDLALAQFELL----SERDIVSWNS 254

Query: 260 VLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLL---QEAYLFIESM 319
           +++ C   G   +  ++++ + +   ++P     A  +        L   ++ + +I   
Sbjct: 255 MIAGCNQHGFDNEALQFFSSILKDTSLKPDRFSLASALSACANLEKLSFGKQIHGYIVRT 314

Query: 320 PIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNSGAYLALANVYSACGKWENAA 379
             +       +L++        ++A+   E+  + D  +  A+ AL N Y   G    A 
Sbjct: 315 MFDASGAVGNALISMYAKSGGVEIARRIIEQSGISDL-DVIAFTALLNGYVKLGDITPAR 374

Query: 380 QTRKLMKDRGV 381
           Q    +KD  V
Sbjct: 375 QIFNSLKDPDV 377


HSP 3 Score: 803.9 bits (2075), Expect = 1.1e-229
Identity = 389/518 (75.10%), Postives = 456/518 (88.03%), Query Frame = 1

Query: 1   MLNEPSLKPDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSG 60
           ML + S KPD FTLAS LSACANLE L +GKQIHA+I+R E +T GAVGNALISMY+KSG
Sbjct: 268 MLMDSSSKPDKFTLASALSACANLENLKLGKQIHAHIIRTEFDTFGAVGNALISMYSKSG 327

Query: 61  GVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGY 120
           GVEIA+ I+E +  SNL++IAFT+LLDGY KLG++ PAR IF+ LR RDVVAWTAMIVGY
Sbjct: 328 GVEIAQKIIEQSMISNLDVIAFTALLDGYVKLGDINPARRIFDSLRVRDVVAWTAMIVGY 387

Query: 121 VQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTP 180
           VQNG   DA+ELFR M+ EGP+PN+YTLA MLSVSSSL  L+HG+QIHASA ++G +S+ 
Sbjct: 388 VQNGFNQDAMELFRSMIKEGPKPNNYTLATMLSVSSSLASLDHGRQIHASATRSGNASSV 447

Query: 181 SVTNALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLS 240
           SV+NALI MYAK+G+IN A+ VF+L + K++ ++WTSMI+ALAQHGLG+EA+ LFERML 
Sbjct: 448 SVSNALITMYAKSGSINDARWVFNLIHWKRDTITWTSMIIALAQHGLGEEALTLFERMLE 507

Query: 241 VGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQ 300
            G+KPDHITYVGVLSACTHVGLVEQGR YYN+M   H+I PT SHYACMIDL+GRAGLLQ
Sbjct: 508 NGIKPDHITYVGVLSACTHVGLVEQGRSYYNLMQNAHKIIPTPSHYACMIDLFGRAGLLQ 567

Query: 301 EAYLFIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNSGAYLALANVYS 360
           EA+ FIE+MPIEPD IAWGSLLASCK+HKN +LA+VAAERLLLI+P NSGAY ALANVYS
Sbjct: 568 EAHAFIENMPIEPDVIAWGSLLASCKVHKNVELAEVAAERLLLIEPENSGAYSALANVYS 627

Query: 361 ACGKWENAAQTRKLMKDRGVRKEKGISWIHIKNEVHAFGVEDVIHPQKDEIYKLMAEIWE 420
           ACG+WENAA  RK MKD+GV+K++G SW+ IKN+VH FGV+D +HPQ+D IY++MA+IW+
Sbjct: 628 ACGQWENAANIRKSMKDKGVKKDQGFSWVQIKNKVHIFGVDDGLHPQRDAIYEMMAKIWK 687

Query: 421 EIKKMGFIPDTESVLHDLEEEVKEQILKYHSEKLAIAFGLLNTPENTALRIMKNLRVCND 480
           EIKKMGF+PDTESVLHDLEEE+KEQIL +HSEKLAIAFGL+ TPENT LRIMKNLRVCND
Sbjct: 688 EIKKMGFVPDTESVLHDLEEELKEQILSHHSEKLAIAFGLICTPENTTLRIMKNLRVCND 747

Query: 481 CHSAIKFISKLVGREIIVRDATRFHHFKDGSCSCRDYW 519
           CHSAIKFISKLVGREIIVRDATRFHHFK+G CSCRDYW
Sbjct: 748 CHSAIKFISKLVGREIIVRDATRFHHFKNGLCSCRDYW 785

BLAST of Csa5G622560.2 vs. TrEMBL
Match: F6H4P3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_07s0031g01340 PE=4 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 4.2e-30
Identity = 96/325 (29.54%), Postives = 169/325 (52.00%), Query Frame = 1

Query: 30  GKQIHAYILRAETETSGAVGNALISMYAKSGGVEIARLIVEHNRTSNLNIIAFTSLLDGY 89
           GK IHA I++A       + N L++ YAK+G +  A  + +     +  + ++  +L GY
Sbjct: 32  GKSIHARIIKAGLHLGVFLMNNLMNFYAKTGFIYDAHRVFDEMPVKS--VFSWNIILSGY 91

Query: 90  TKLGNVKPAREIFNKLRDRDVVAWTAMIVGYVQNGLWNDALELFRLMVNEGPEPNSYTLA 149
            K G ++ A  +F ++ + D V+WTAMIVGY Q G + +A+ +FR MV++   P  +TL 
Sbjct: 92  AKGGRLEEAHRVFEEMPEPDSVSWTAMIVGYNQMGQFENAIGMFREMVSDDVPPTQFTLT 151

Query: 150 AMLSVSSSLTILEHGKQIHASAIKAGESSTPSVTNALIAMYAKTG--------------- 209
            +L+  +++  L  G+++H+  +K G SS  SV N+L+ MYAK+G               
Sbjct: 152 NVLASCAAVECLGIGRKVHSFVVKHGLSSYISVANSLLNMYAKSGDPVTAKIVFDRMKLK 211

Query: 210 ---------NINVAKRVFDLPNGK------KEIVSWTSMIMALAQHGLGKEAINLFERML 269
                    + ++   + DL   +      +++VSW +MI    QHG  +EA+++F +ML
Sbjct: 212 STSSWNTMISSHMQSGLVDLAQVQFEQMIERDVVSWNAMISGYNQHGFDREALDIFSKML 271

Query: 270 -SVGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGL 323
                KPD  T    LSAC ++  ++ G++ +  +    E +   +    +I +Y ++G 
Sbjct: 272 MDSSSKPDKFTLASALSACANLENLKLGKQIHAHIIRT-EFDTFGAVGNALISMYSKSGG 331


HSP 2 Score: 74.3 bits (181), Expect = 4.8e-10
Identity = 60/209 (28.71%), Postives = 99/209 (47.37%), Query Frame = 1

Query: 164 GKQIHASAIKAGESSTPSVTNALIAMYAKTGNINVAKRVFD-LP---------------- 223
           GK IHA  IKAG      + N L+  YAKTG I  A RVFD +P                
Sbjct: 32  GKSIHARIIKAGLHLGVFLMNNLMNFYAKTGFIYDAHRVFDEMPVKSVFSWNIILSGYAK 91

Query: 224 NGKKE-------------IVSWTSMIMALAQHGLGKEAINLFERMLSVGMKPDHITYVGV 283
            G+ E              VSWT+MI+   Q G  + AI +F  M+S  + P   T   V
Sbjct: 92  GGRLEEAHRVFEEMPEPDSVSWTAMIVGYNQMGQFENAIGMFREMVSDDVPPTQFTLTNV 151

Query: 284 LSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQEAYLFIESMPIEP 343
           L++C  V  +  GRK ++ + + H +   +S    ++++Y ++G    A +  + M ++ 
Sbjct: 152 LASCAAVECLGIGRKVHSFVVK-HGLSSYISVANSLLNMYAKSGDPVTAKIVFDRMKLKS 211


HSP 3 Score: 801.2 bits (2068), Expect = 7.4e-229
Identity = 387/518 (74.71%), Postives = 455/518 (87.84%), Query Frame = 1

Query: 1   MLNEPSLKPDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSG 60
           ML + SLKPD FTLAS LSAC NLE +N+GKQIH+YI+R E + SGAV NALISMYAK+G
Sbjct: 146 MLQDSSLKPDKFTLASTLSACTNLENMNLGKQIHSYIIRTEFDISGAVQNALISMYAKTG 205

Query: 61  GVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGY 120
           GVE+AR +VE +  S+L+ IAFT+LLDGY KLG++ PAR+IF+ LR+RDVVAWTAMIVGY
Sbjct: 206 GVEVARRVVEQSEISDLDAIAFTALLDGYAKLGDITPARQIFDSLRNRDVVAWTAMIVGY 265

Query: 121 VQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTP 180
           VQNGL +DALELFR M  EGP PNS+TLAAMLSVSS    L HGKQIHASAI++ E  + 
Sbjct: 266 VQNGLNDDALELFRAMTKEGPRPNSFTLAAMLSVSSRGASLNHGKQIHASAIRSREDLSI 325

Query: 181 SVTNALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLS 240
           SV NALI MYAK G+I+ A++VF+L     + VSW+SMI+ALAQHGLG+EAI LFE+ML+
Sbjct: 326 SVGNALITMYAKAGSIDNAQKVFNLICWNNDTVSWSSMIIALAQHGLGEEAIELFEKMLA 385

Query: 241 VGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQ 300
           +G++PDHITYVGVLSACTHVGLVEQGR Y+N+M  VH+IEPTLSHYACMIDLYGRAGLLQ
Sbjct: 386 LGIEPDHITYVGVLSACTHVGLVEQGRSYFNLMASVHKIEPTLSHYACMIDLYGRAGLLQ 445

Query: 301 EAYLFIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNSGAYLALANVYS 360
           EA+ FIE+MPIEPD IAWGSLL+SCK++KN DLAK+AAERLLLI+P NSGAY ALANVYS
Sbjct: 446 EAFKFIENMPIEPDVIAWGSLLSSCKVYKNVDLAKIAAERLLLIEPDNSGAYSALANVYS 505

Query: 361 ACGKWENAAQTRKLMKDRGVRKEKGISWIHIKNEVHAFGVEDVIHPQKDEIYKLMAEIWE 420
           ACGKWE+AA+ RKLM DRGV+KE+G SW+ I+NEVH FG ED +HPQKDEIYK+M +IWE
Sbjct: 506 ACGKWEDAAKIRKLMNDRGVKKEQGSSWLEIQNEVHIFGAEDSLHPQKDEIYKMMDKIWE 565

Query: 421 EIKKMGFIPDTESVLHDLEEEVKEQILKYHSEKLAIAFGLLNTPENTALRIMKNLRVCND 480
           EIKK+GFIPDT+SVLHDLE EVK+++L+YHSEKLAIAFG+++TPENT LRIMKNLR+CND
Sbjct: 566 EIKKLGFIPDTDSVLHDLEIEVKDKLLRYHSEKLAIAFGIISTPENTTLRIMKNLRICND 625

Query: 481 CHSAIKFISKLVGREIIVRDATRFHHFKDGSCSCRDYW 519
           CH+AIKFISKL+GREIIVRD TRFHHFKDG CSCRDYW
Sbjct: 626 CHTAIKFISKLMGREIIVRDVTRFHHFKDGFCSCRDYW 663

BLAST of Csa5G622560.2 vs. TrEMBL
Match: A0A067KG88_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_09370 PE=4 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 1.3e-31
Identity = 82/271 (30.26%), Postives = 156/271 (57.56%), Query Frame = 1

Query: 7   LKPDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSGGVEIAR 66
           + P  FT+ ++LS+CA L  L+IG++IH+++++       +VGN+L++MYAK+G + +A+
Sbjct: 19  VSPSQFTITNVLSSCAALRALDIGRKIHSFVVKLGLSGYASVGNSLLNMYAKAGDLVMAK 78

Query: 67  LIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGYVQNGLW 126
           ++ +  R    +I ++  ++  +   G +  A   F ++ +RD+V W +MI GY Q+G  
Sbjct: 79  IVFDRMRLR--SITSWNIMISLHMNSGRIDLALAQFQQMSERDIVTWNSMIAGYNQHGFD 138

Query: 127 NDALELFRLMVNEGP-EPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTPSVTNA 186
            +ALELF  M+ +   +P+ +TLA+ LS  ++L  +  GKQIH+  I+     + +V NA
Sbjct: 139 KEALELFSSMLQDSSLKPDKFTLASTLSACTNLENMNLGKQIHSYIIRTEFDISGAVQNA 198

Query: 187 LIAMYAKTGNINVAKRVFDLPN-GKKEIVSWTSMIMALAQHGLGKEAINLFERMLSVGMK 246
           LI+MYAKTG + VA+RV +       + +++T+++   A+ G    A  +F+ +      
Sbjct: 199 LISMYAKTGGVEVARRVVEQSEISDLDAIAFTALLDGYAKLGDITPARQIFDSL----RN 258

Query: 247 PDHITYVGVLSACTHVGLVEQGRKYYNMMTE 276
            D + +  ++      GL +   + +  MT+
Sbjct: 259 RDVVAWTAMIVGYVQNGLNDDALELFRAMTK 283


HSP 2 Score: 99.8 bits (247), Expect = 1.1e-17
Identity = 69/229 (30.13%), Postives = 119/229 (51.97%), Query Frame = 1

Query: 126 WNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTPSVTNA 185
           + +A+++F  MV +   P+ +T+  +LS  ++L  L+ G++IH+  +K G S   SV N+
Sbjct: 4   FENAIKMFVDMVKDKVSPSQFTITNVLSSCAALRALDIGRKIHSFVVKLGLSGYASVGNS 63

Query: 186 LIAMYAKTGNINVAKRVFD-----------------LPNGK-------------KEIVSW 245
           L+ MYAK G++ +AK VFD                 + +G+             ++IV+W
Sbjct: 64  LLNMYAKAGDLVMAKIVFDRMRLRSITSWNIMISLHMNSGRIDLALAQFQQMSERDIVTW 123

Query: 246 TSMIMALAQHGLGKEAINLFERML-SVGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMT 305
            SMI    QHG  KEA+ LF  ML    +KPD  T    LSACT++  +  G++ ++ + 
Sbjct: 124 NSMIAGYNQHGFDKEALELFSSMLQDSSLKPDKFTLASTLSACTNLENMNLGKQIHSYII 183

Query: 306 EVHEIEPTLSHYACMIDLYGRAGLLQEAYLFIESMPI-EPDNIAWGSLL 323
              E + + +    +I +Y + G ++ A   +E   I + D IA+ +LL
Sbjct: 184 RT-EFDISGAVQNALISMYAKTGGVEVARRVVEQSEISDLDAIAFTALL 231


HSP 3 Score: 71.2 bits (173), Expect = 4.1e-09
Identity = 61/258 (23.64%), Postives = 123/258 (47.67%), Query Frame = 1

Query: 126 WNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTPSVTNA 185
           + +A+++F  MV +   P+ +T+  +LS  ++L  L+ G++IH+  +K G S   SV N+
Sbjct: 4   FENAIKMFVDMVKDKVSPSQFTITNVLSSCAALRALDIGRKIHSFVVKLGLSGYASVGNS 63

Query: 186 LIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLSVGMKP 245
           L+ MYAK G++ +AK VFD     + I SW  MI      G    A+  F++M     + 
Sbjct: 64  LLNMYAKAGDLVMAKIVFDRMR-LRSITSWNIMISLHMNSGRIDLALAQFQQM----SER 123

Query: 246 DHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEP---TLSHYACMIDLYGRAGLLQEA 305
           D +T+  +++     G  ++  + ++ M +   ++P   TL+             L ++ 
Sbjct: 124 DIVTWNSMIAGYNQHGFDKEALELFSSMLQDSSLKPDKFTLASTLSACTNLENMNLGKQI 183

Query: 306 YLFIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNSGAYLALANVYSAC 365
           + +I     +       +L++        ++A+   E+  + D  ++ A+ AL + Y+  
Sbjct: 184 HSYIIRTEFDISGAVQNALISMYAKTGGVEVARRVVEQSEISDL-DAIAFTALLDGYAKL 243

Query: 366 GKWENAAQTRKLMKDRGV 381
           G    A Q    +++R V
Sbjct: 244 GDITPARQIFDSLRNRDV 255


HSP 4 Score: 800.4 bits (2066), Expect = 1.3e-228
Identity = 388/518 (74.90%), Postives = 451/518 (87.07%), Query Frame = 1

Query: 1   MLNEPSLKPDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSG 60
           ML +  L PD FT  S LSACANLE L +GKQIHA I+  + +T G VGNALISMYAKSG
Sbjct: 265 MLRDSLLLPDKFTFISALSACANLEMLKLGKQIHARIVSTKFDTYGPVGNALISMYAKSG 324

Query: 61  GVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGY 120
           GV+IA+ IVE +  S+L++IAFTSLLDGY KLG++KPAREIF+ LRDRDVVAWTAMIVGY
Sbjct: 325 GVQIAQKIVEKSGISHLDVIAFTSLLDGYIKLGDLKPAREIFDTLRDRDVVAWTAMIVGY 384

Query: 121 VQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTP 180
           +QNGL NDALELFRLMV +GP+PN++TLAAMLSVSSSLT L+HGKQIHASAI+ G++S+ 
Sbjct: 385 LQNGLNNDALELFRLMVRDGPKPNNFTLAAMLSVSSSLTSLDHGKQIHASAIRTGQASSV 444

Query: 181 SVTNALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLS 240
           SV NALI MY++ GNIN A+++F   +  ++ VSWTSMIM LAQHGLG+EA+ LFE +L+
Sbjct: 445 SVNNALITMYSRAGNINCARQIFSQIHWSRDTVSWTSMIMGLAQHGLGEEALELFEELLA 504

Query: 241 VGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQ 300
            G+KPDHITYVGVLSACTHVGLVEQG +YYNMM + H+IEPTLSHYA M+DL GRAGL+Q
Sbjct: 505 AGIKPDHITYVGVLSACTHVGLVEQGHRYYNMMKDFHKIEPTLSHYALMVDLLGRAGLVQ 564

Query: 301 EAYLFIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNSGAYLALANVYS 360
           EAY FIE MPIEPD I WGSLL+SCK++KN DL KVAAERLL IDP NSGAY ALAN+YS
Sbjct: 565 EAYDFIEKMPIEPDVITWGSLLSSCKVYKNVDLGKVAAERLLCIDPDNSGAYSALANLYS 624

Query: 361 ACGKWENAAQTRKLMKDRGVRKEKGISWIHIKNEVHAFGVEDVIHPQKDEIYKLMAEIWE 420
            CGKWE+AA+ RKLMKD GV+KE+GISW+ IKN+VH FG ED +HPQKDE+YK+MA+IWE
Sbjct: 625 VCGKWEDAAKIRKLMKDGGVKKEQGISWVQIKNKVHIFGAEDGLHPQKDEVYKMMAKIWE 684

Query: 421 EIKKMGFIPDTESVLHDLEEEVKEQILKYHSEKLAIAFGLLNTPENTALRIMKNLRVCND 480
           +IKKMGF+PDTESVLHDLEEEVKEQ+LK+HSEKLAIAF L++TPENT LRIMKNLRVCND
Sbjct: 685 DIKKMGFVPDTESVLHDLEEEVKEQMLKHHSEKLAIAFALISTPENTTLRIMKNLRVCND 744

Query: 481 CHSAIKFISKLVGREIIVRDATRFHHFKDGSCSCRDYW 519
           CHSAIKFISKL GREII+RDATRFHHFKDG CSC DYW
Sbjct: 745 CHSAIKFISKLSGREIIIRDATRFHHFKDGLCSCCDYW 782

BLAST of Csa5G622560.2 vs. TrEMBL
Match: A0A061DIY7_THECC (Pentatricopeptide (PPR) repeat-containing protein OS=Theobroma cacao GN=TCM_001214 PE=4 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 1.6e-26
Identity = 73/232 (31.47%), Postives = 137/232 (59.05%), Query Frame = 1

Query: 9   PDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSGGVEIARLI 68
           P  +T+ S+L++CA +E L+IG+++H+ +++    +  +V N+L++MYAKSG   I  +I
Sbjct: 140 PTQYTITSVLASCAAIETLDIGRKVHSCVVKFGLSSYVSVANSLLNMYAKSGDPMITGVI 199

Query: 69  VEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGYVQNGLWND 128
            +  R    +  ++  ++  + + G V  ARE F ++ +RD+V W +MI GY Q+G   D
Sbjct: 200 FD--RMGYRDTPSWNVMISLHMQYGQVDLAREQFCRMNERDIVTWNSMIAGYNQHGFDLD 259

Query: 129 ALELFRLMVNEG-PEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTPSVTNALI 188
           AL  F  M+ +    P+ +T  + LS  ++L +L+ GKQIHA  +     +   V NALI
Sbjct: 260 ALGTFGNMLRDSLLLPDKFTFISALSACANLEMLKLGKQIHARIVSTKFDTYGPVGNALI 319

Query: 189 AMYAKTGNINVAKRVFDLPN-GKKEIVSWTSMIMALAQHGLGKEAINLFERM 239
           +MYAK+G + +A+++ +       +++++TS++    + G  K A  +F+ +
Sbjct: 320 SMYAKSGGVQIAQKIVEKSGISHLDVIAFTSLLDGYIKLGDLKPAREIFDTL 369


HSP 2 Score: 127.1 bits (318), Expect = 6.2e-26
Identity = 78/237 (32.91%), Postives = 130/237 (54.85%), Query Frame = 1

Query: 2   LNEPSLKPDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSGG 61
           +N P     ++  A +L      + L  GK IHA I++A    S  + N L+++Y+K+G 
Sbjct: 1   MNIPDPSSLSYHTACLLQESLRSKDLLAGKSIHARIIKAGLHFSVFLLNNLMNVYSKTGS 60

Query: 62  VEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGYV 121
              A+ + +        I ++ +LL  Y+K G +  A EIFNK+ + D V+W+ MIVGY 
Sbjct: 61  FYDAQSLFDEMPVKT--IFSWNTLLSAYSKQGKMAEANEIFNKIPNPDSVSWSTMIVGYN 120

Query: 122 QNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTPS 181
           Q G +  A+++F  M+ E   P  YT+ ++L+  +++  L+ G+++H+  +K G SS  S
Sbjct: 121 QMGHFESAIKVFDEMIKERVTPTQYTITSVLASCAAIETLDIGRKVHSCVVKFGLSSYVS 180

Query: 182 VTNALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERM 239
           V N+L+ MYAK+G+  +   +FD   G ++  SW  MI    Q+G    A   F RM
Sbjct: 181 VANSLLNMYAKSGDPMITGVIFD-RMGYRDTPSWNVMISLHMQYGQVDLAREQFCRM 234


HSP 3 Score: 56.6 bits (135), Expect = 1.0e-04
Identity = 37/153 (24.18%), Postives = 78/153 (50.98%), Query Frame = 1

Query: 184 NALIAMYAKTGNINVAKRVFD-LPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLSVG 243
           N L++ Y+K G +  A  +F+ +PN   + VSW++MI+   Q G  + AI +F+ M+   
Sbjct: 80  NTLLSAYSKQGKMAEANEIFNKIPN--PDSVSWSTMIVGYNQMGHFESAIKVFDEMIKER 139

Query: 244 MKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQEA 303
           + P   T   VL++C  +  ++ GRK ++ + +   +   +S    ++++Y ++G     
Sbjct: 140 VTPTQYTITSVLASCAAIETLDIGRKVHSCVVK-FGLSSYVSVANSLLNMYAKSGDPMIT 199

Query: 304 YLFIESMPIEPDNIAWGSLLASCKIHKNADLAK 336
            +  + M    D  +W  +++    +   DLA+
Sbjct: 200 GVIFDRMGYR-DTPSWNVMISLHMQYGQVDLAR 228


HSP 4 Score: 796.6 bits (2056), Expect = 1.8e-227
Identity = 387/518 (74.71%), Postives = 450/518 (86.87%), Query Frame = 1

Query: 1   MLNEPSLKPDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSG 60
           ML E SL+PD +TLAS LSACANL +LN+GKQIHAY++R E  TSGAVGN+LI MY++SG
Sbjct: 196 MLKESSLEPDRYTLASALSACANLGELNVGKQIHAYLVRTEFNTSGAVGNSLICMYSRSG 255

Query: 61  GVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGY 120
           GV+IAR I+E NR SNLN+IAFT+LL+GY KLG++ PAR+IF+ L+DRDVV WTAMIVGY
Sbjct: 256 GVDIARRILEKNRESNLNVIAFTALLNGYIKLGDINPARKIFDSLKDRDVVVWTAMIVGY 315

Query: 121 VQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTP 180
           VQNG  +DA+ELFRLMV EGP+PN+YTLAAMLSV SS+  L HGKQIH++AIKAGE+ + 
Sbjct: 316 VQNGFNDDAMELFRLMVKEGPDPNNYTLAAMLSVCSSVASLNHGKQIHSAAIKAGEALSV 375

Query: 181 SVTNALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLS 240
           SV+NALI MYAK GNI+ A+RVFDL +  ++ VSWTSMI+ALAQHGLG EA+ LFE ML+
Sbjct: 376 SVSNALITMYAKAGNISCARRVFDLIHLNRDTVSWTSMILALAQHGLGAEALQLFENMLA 435

Query: 241 VGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQ 300
           +GMKPDHITYVGVL+ACTHVGL+ QGR YY MM E+H IEPT SH ACMIDL+GRAGLL+
Sbjct: 436 LGMKPDHITYVGVLTACTHVGLIAQGRSYYKMMKEIHGIEPTSSHCACMIDLFGRAGLLE 495

Query: 301 EAYLFIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNSGAYLALANVYS 360
           EA  FIE+MPIEPD IAWGSLLASC++HK  +LAKVAA+RLL IDP NSGAY ALANVYS
Sbjct: 496 EAQDFIENMPIEPDVIAWGSLLASCRVHKKMELAKVAADRLLSIDPENSGAYSALANVYS 555

Query: 361 ACGKWENAAQTRKLMKDRGVRKEKGISWIHIKNEVHAFGVEDVIHPQKDEIYKLMAEIWE 420
           ACGKW  AA+ RK MKD+ V+KE+G SWI IKN VH FGVED +HPQ+D IYK M +IW+
Sbjct: 556 ACGKWAEAAKIRKSMKDKQVKKEQGFSWIQIKNVVHVFGVEDGLHPQRDAIYKTMEKIWK 615

Query: 421 EIKKMGFIPDTESVLHDLEEEVKEQILKYHSEKLAIAFGLLNTPENTALRIMKNLRVCND 480
           +IKK+GFIPDTESVLHDL+ EVKEQIL++HSEKLAIAFGL+NTPE T LRIMKNLRVCND
Sbjct: 616 DIKKLGFIPDTESVLHDLDYEVKEQILRHHSEKLAIAFGLINTPEKTTLRIMKNLRVCND 675

Query: 481 CHSAIKFISKLVGREIIVRDATRFHHFKDGSCSCRDYW 519
           CHSAIKFISKLVGREII+RDATRFHHFK G CSCRDYW
Sbjct: 676 CHSAIKFISKLVGREIILRDATRFHHFKGGFCSCRDYW 713

BLAST of Csa5G622560.2 vs. TAIR10
Match: AT2G22070.1 (AT2G22070.1 pentatricopeptide (PPR) repeat-containing protein)

HSP 1 Score: 734.6 bits (1895), Expect = 4.3e-212
Identity = 358/518 (69.11%), Postives = 422/518 (81.47%), Query Frame = 1

Query: 1   MLNEPSLKPDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSG 60
           ML +  L PD FTLAS+LSACANLEKL IGKQIH++I+    + SG V NALISMY++ G
Sbjct: 269 MLRDSLLSPDRFTLASVLSACANLEKLCIGKQIHSHIVTTGFDISGIVLNALISMYSRCG 328

Query: 61  GVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGY 120
           GVE AR ++E   T +L I  FT+LLDGY KLG++  A+ IF  L+DRDVVAWTAMIVGY
Sbjct: 329 GVETARRLIEQRGTKDLKIEGFTALLDGYIKLGDMNQAKNIFVSLKDRDVVAWTAMIVGY 388

Query: 121 VQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTP 180
            Q+G + +A+ LFR MV  G  PNSYTLAAMLSV+SSL  L HGKQIH SA+K+GE  + 
Sbjct: 389 EQHGSYGEAINLFRSMVGGGQRPNSYTLAAMLSVASSLASLSHGKQIHGSAVKSGEIYSV 448

Query: 181 SVTNALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLS 240
           SV+NALI MYAK GNI  A R FDL   +++ VSWTSMI+ALAQHG  +EA+ LFE ML 
Sbjct: 449 SVSNALITMYAKAGNITSASRAFDLIRCERDTVSWTSMIIALAQHGHAEEALELFETMLM 508

Query: 241 VGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQ 300
            G++PDHITYVGV SACTH GLV QGR+Y++MM +V +I PTLSHYACM+DL+GRAGLLQ
Sbjct: 509 EGLRPDHITYVGVFSACTHAGLVNQGRQYFDMMKDVDKIIPTLSHYACMVDLFGRAGLLQ 568

Query: 301 EAYLFIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNSGAYLALANVYS 360
           EA  FIE MPIEPD + WGSLL++C++HKN DL KVAAERLLL++P NSGAY ALAN+YS
Sbjct: 569 EAQEFIEKMPIEPDVVTWGSLLSACRVHKNIDLGKVAAERLLLLEPENSGAYSALANLYS 628

Query: 361 ACGKWENAAQTRKLMKDRGVRKEKGISWIHIKNEVHAFGVEDVIHPQKDEIYKLMAEIWE 420
           ACGKWE AA+ RK MKD  V+KE+G SWI +K++VH FGVED  HP+K+EIY  M +IW+
Sbjct: 629 ACGKWEEAAKIRKSMKDGRVKKEQGFSWIEVKHKVHVFGVEDGTHPEKNEIYMTMKKIWD 688

Query: 421 EIKKMGFIPDTESVLHDLEEEVKEQILKYHSEKLAIAFGLLNTPENTALRIMKNLRVCND 480
           EIKKMG++PDT SVLHDLEEEVKEQIL++HSEKLAIAFGL++TP+ T LRIMKNLRVCND
Sbjct: 689 EIKKMGYVPDTASVLHDLEEEVKEQILRHHSEKLAIAFGLISTPDKTTLRIMKNLRVCND 748

Query: 481 CHSAIKFISKLVGREIIVRDATRFHHFKDGSCSCRDYW 519
           CH+AIKFISKLVGREIIVRD TRFHHFKDG CSCRDYW
Sbjct: 749 CHTAIKFISKLVGREIIVRDTTRFHHFKDGFCSCRDYW 786


HSP 2 Score: 117.9 bits (294), Expect = 1.9e-26
Identity = 81/263 (30.80%), Postives = 139/263 (52.85%), Query Frame = 1

Query: 9   PDNFTLASILSACANLEKLNIGKQ--------IHAYILRAETETSGAVGNALISMYAKSG 68
           P   +L+++L  C NL + ++ K         +H  ++++    S  + N L+++Y+K+G
Sbjct: 4   PVPLSLSTLLELCTNLLQKSVNKSNGRFTAQLVHCRVIKSGLMFSVYLMNNLMNVYSKTG 63

Query: 69  GVEIARLIVEHN--RTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIV 128
               AR + +    RT+     ++ ++L  Y+K G++    E F++L  RD V+WT MIV
Sbjct: 64  YALHARKLFDEMPLRTA----FSWNTVLSAYSKRGDMDSTCEFFDQLPQRDSVSWTTMIV 123

Query: 129 GYVQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESS 188
           GY   G ++ A+ +   MV EG EP  +TL  +L+  ++   +E GK++H+  +K G   
Sbjct: 124 GYKNIGQYHKAIRVMGDMVKEGIEPTQFTLTNVLASVAATRCMETGKKVHSFIVKLGLRG 183

Query: 189 TPSVTNALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERM 248
             SV+N+L+ MYAK G+  +AK VFD     ++I SW +MI    Q G    A+  FE+M
Sbjct: 184 NVSVSNSLLNMYAKCGDPMMAKFVFD-RMVVRDISSWNAMIALHMQVGQMDLAMAQFEQM 243

Query: 249 LSVGMKPDHITYVGVLSACTHVG 262
                + D +T+  ++S     G
Sbjct: 244 ----AERDIVTWNSMISGFNQRG 257


HSP 3 Score: 116.3 bits (290), Expect = 5.6e-26
Identity = 68/231 (29.44%), Postives = 133/231 (57.58%), Query Frame = 1

Query: 7   LKPDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSGGVEIAR 66
           ++P  FTL ++L++ A    +  GK++H++I++     + +V N+L++MYAK G   +A+
Sbjct: 142 IEPTQFTLTNVLASVAATRCMETGKKVHSFIVKLGLRGNVSVSNSLLNMYAKCGDPMMAK 201

Query: 67  LIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGYVQNGLW 126
            + +  R    +I ++ +++  + ++G +  A   F ++ +RD+V W +MI G+ Q G  
Sbjct: 202 FVFD--RMVVRDISSWNAMIALHMQVGQMDLAMAQFEQMAERDIVTWNSMISGFNQRGYD 261

Query: 127 NDALELFRLMVNEG-PEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTPSVTNA 186
             AL++F  M+ +    P+ +TLA++LS  ++L  L  GKQIH+  +  G   +  V NA
Sbjct: 262 LRALDIFSKMLRDSLLSPDRFTLASVLSACANLEKLCIGKQIHSHIVTTGFDISGIVLNA 321

Query: 187 LIAMYAKTGNINVAKRVFDLPNGKK-EIVSWTSMIMALAQHGLGKEAINLF 236
           LI+MY++ G +  A+R+ +    K  +I  +T+++    + G   +A N+F
Sbjct: 322 LISMYSRCGGVETARRLIEQRGTKDLKIEGFTALLDGYIKLGDMNQAKNIF 370

BLAST of Csa5G622560.2 vs. TAIR10
Match: AT1G25360.1 (AT1G25360.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 444.9 bits (1143), Expect = 6.8e-125
Identity = 218/511 (42.66%), Postives = 331/511 (64.77%), Query Frame = 1

Query: 10  DNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSGGVEIARLIV 69
           D FT  S++ ACA    L +GKQ+HAY+LR E + S    N+L+S+Y K G  + AR I 
Sbjct: 285 DEFTYPSVIRACATAGLLQLGKQVHAYVLRRE-DFSFHFDNSLVSLYYKCGKFDEARAIF 344

Query: 70  EHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGYVQNGLWNDA 129
           E     +L  +++ +LL GY   G++  A+ IF +++++++++W  MI G  +NG   + 
Sbjct: 345 EKMPAKDL--VSWNALLSGYVSSGHIGEAKLIFKEMKEKNILSWMIMISGLAENGFGEEG 404

Query: 130 LELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTPSVTNALIAM 189
           L+LF  M  EG EP  Y  +  +   + L    +G+Q HA  +K G  S+ S  NALI M
Sbjct: 405 LKLFSCMKREGFEPCDYAFSGAIKSCAVLGAYCNGQQYHAQLLKIGFDSSLSAGNALITM 464

Query: 190 YAKTGNINVAKRVF-DLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLSVGMKPDHI 249
           YAK G +  A++VF  +P    + VSW ++I AL QHG G EA++++E ML  G++PD I
Sbjct: 465 YAKCGVVEEARQVFRTMPC--LDSVSWNALIAALGQHGHGAEAVDVYEEMLKKGIRPDRI 524

Query: 250 TYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQEAYLFIES 309
           T + VL+AC+H GLV+QGRKY++ M  V+ I P   HYA +IDL  R+G   +A   IES
Sbjct: 525 TLLTVLTACSHAGLVDQGRKYFDSMETVYRIPPGADHYARLIDLLCRSGKFSDAESVIES 584

Query: 310 MPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNSGAYLALANVYSACGKWENA 369
           +P +P    W +LL+ C++H N +L  +AA++L  + P + G Y+ L+N+++A G+WE  
Sbjct: 585 LPFKPTAEIWEALLSGCRVHGNMELGIIAADKLFGLIPEHDGTYMLLSNMHAATGQWEEV 644

Query: 370 AQTRKLMKDRGVRKEKGISWIHIKNEVHAFGVEDVIHPQKDEIYKLMAEIWEEIKKMGFI 429
           A+ RKLM+DRGV+KE   SWI ++ +VH F V+D  HP+ + +Y  + ++ +E++++G++
Sbjct: 645 ARVRKLMRDRGVKKEVACSWIEMETQVHTFLVDDTSHPEAEAVYIYLQDLGKEMRRLGYV 704

Query: 430 PDTESVLHDLEEE-VKEQILKYHSEKLAIAFGLLNTPENTALRIMKNLRVCNDCHSAIKF 489
           PDT  VLHD+E +  KE +L  HSEK+A+AFGL+  P  T +RI KNLR C DCH+  +F
Sbjct: 705 PDTSFVLHDVESDGHKEDMLTTHSEKIAVAFGLMKLPPGTTIRIFKNLRTCGDCHNFFRF 764

Query: 490 ISKLVGREIIVRDATRFHHFKDGSCSCRDYW 519
           +S +V R+II+RD  RFHHF++G CSC ++W
Sbjct: 765 LSWVVQRDIILRDRKRFHHFRNGECSCGNFW 790


HSP 2 Score: 147.1 bits (370), Expect = 2.9e-35
Identity = 111/410 (27.07%), Postives = 193/410 (47.07%), Query Frame = 1

Query: 2   LNEPSLKPDNFTLASILSACANL---EKLNIGKQIHAYILRAETETSGAVGNALISMYAK 61
           +     KPDNFT AS+L+  A +   EK  +  Q HA  L++      +V NAL+S+Y+K
Sbjct: 139 MKHEGFKPDNFTFASVLAGLALVADDEKQCV--QFHAAALKSGAGYITSVSNALVSVYSK 198

Query: 62  SGG----VEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDR-DVVAW 121
                  +  AR + +     +    ++T+++ GY K G      E+   + D   +VA+
Sbjct: 199 CASSPSLLHSARKVFDEILEKDER--SWTTMMTGYVKNGYFDLGEELLEGMDDNMKLVAY 258

Query: 122 TAMIVGYVQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIK 181
            AMI GYV  G + +ALE+ R MV+ G E + +T  +++   ++  +L+ GKQ+HA  ++
Sbjct: 259 NAMISGYVNRGFYQEALEMVRRMVSSGIELDEFTYPSVIRACATAGLLQLGKQVHAYVLR 318

Query: 182 AGESSTPSVTNALIAMYAKTGNINVAKRVFDLPNGK------------------------ 241
             + S     N+L+++Y K G  + A+ +F+    K                        
Sbjct: 319 REDFSF-HFDNSLVSLYYKCGKFDEARAIFEKMPAKDLVSWNALLSGYVSSGHIGEAKLI 378

Query: 242 ------KEIVSWTSMIMALAQHGLGKEAINLFERMLSVGMKPDHITYVGVLSACTHVGLV 301
                 K I+SW  MI  LA++G G+E + LF  M   G +P    + G + +C  +G  
Sbjct: 379 FKEMKEKNILSWMIMISGLAENGFGEEGLKLFSCMKREGFEPCDYAFSGAIKSCAVLGAY 438

Query: 302 EQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQEAYLFIESMPIEPDNIAWGSLLA 361
             G++Y+  + ++   + +LS    +I +Y + G+++EA     +MP   D+++W +L+A
Sbjct: 439 CNGQQYHAQLLKI-GFDSSLSAGNALITMYAKCGVVEEARQVFRTMPC-LDSVSWNALIA 498

Query: 362 SCKIHKNADLAKVAAERLLLIDPGNSGAYLALANVYSACGKWENAAQTRK 374
           +   H +   A    E +L    G     + L  V +AC       Q RK
Sbjct: 499 ALGQHGHGAEAVDVYEEML--KKGIRPDRITLLTVLTACSHAGLVDQGRK 539


HSP 3 Score: 97.8 bits (242), Expect = 2.1e-20
Identity = 85/298 (28.52%), Postives = 146/298 (48.99%), Query Frame = 1

Query: 15  ASILSACANLEK--LNIGKQIHAYILRAETETSGAVGNALISMYAKSGGVEIARLIVEHN 74
           A+ L  C  L +  L + + +H  I+    +    + N LI +Y KS  +  AR + +  
Sbjct: 16  AANLRLCLPLRRTSLQLARAVHGNIITFGFQPRAHILNRLIDVYCKSSELNYARQLFDE- 75

Query: 75  RTSNLNIIAFTSLLDGYTKLGNVKPAREIFNK--LRDRDVVAWTAMIVGYVQNGLWNDAL 134
             S  + IA T+++ GY   G++  AR +F K  +  RD V + AMI G+  N     A+
Sbjct: 76  -ISEPDKIARTTMVSGYCASGDITLARGVFEKAPVCMRDTVMYNAMITGFSHNNDGYSAI 135

Query: 135 ELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHG-KQIHASAIKAGESSTPSVTNALIAM 194
            LF  M +EG +P+++T A++L+  + +   E    Q HA+A+K+G     SV+NAL+++
Sbjct: 136 NLFCKMKHEGFKPDNFTFASVLAGLALVADDEKQCVQFHAAALKSGAGYITSVSNALVSV 195

Query: 195 YAKTGN----INVAKRVFDLPNGKKEIVSWTSMIMALAQHG---LGKEAINLFERMLSVG 254
           Y+K  +    ++ A++VFD    K E  SWT+M+    ++G   LG+E +   +  + + 
Sbjct: 196 YSKCASSPSLLHSARKVFDEILEKDE-RSWTTMMTGYVKNGYFDLGEELLEGMDDNMKL- 255

Query: 255 MKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQ 301
                + Y  ++S   + G  ++  +    M     IE     Y  +I     AGLLQ
Sbjct: 256 -----VAYNAMISGYVNRGFYQEALEMVRRMVS-SGIELDEFTYPSVIRACATAGLLQ 303


HSP 4 Score: 61.2 bits (147), Expect = 2.1e-09
Identity = 81/356 (22.75%), Postives = 149/356 (41.85%), Query Frame = 1

Query: 98  AREIFNKLRDRDVVAWTAMIVGYVQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSS 157
           AR++F+++ ++D  +WT M+ GYV+NG ++   EL      EG + N   +A    +S  
Sbjct: 207 ARKVFDEILEKDERSWTTMMTGYVKNGYFDLGEELL-----EGMDDNMKLVAYNAMISGY 266

Query: 158 LT------ILEHGKQIHASAIKAGESSTPSVTNALIAMYAKTGNINVAKRVFDLPNGKKE 217
           +        LE  +++ +S I+  E + PSV  A     A  G + + K+V      +++
Sbjct: 267 VNRGFYQEALEMVRRMVSSGIELDEFTYPSVIRAC----ATAGLLQLGKQVHAYVLRRED 326

Query: 218 IVSW--TSMIMALAQHGLGKEAINLFERMLSVGMKPDHITYVGVLSACTHVGLVEQGRKY 277
                  S++    + G   EA  +FE+M +     D +++  +LS     G + + +  
Sbjct: 327 FSFHFDNSLVSLYYKCGKFDEARAIFEKMPA----KDLVSWNALLSGYVSSGHIGEAKLI 386

Query: 278 YNMMTEVHEIEPTLSHYACMIDLYGRAGLLQEAYLFIESMPIEPDNIAWGSLLASCKI-- 337
           +  M E + +   +       + +G  GL  + +  ++    EP + A+   + SC +  
Sbjct: 387 FKEMKEKNILSWMIMISGLAENGFGEEGL--KLFSCMKREGFEPCDYAFSGAIKSCAVLG 446

Query: 338 ------HKNADLAKVAAERLLLIDPGNSGAYLALANVYSACGKWENAAQTRKLMKDRGVR 397
                   +A L K+  +  L    GN     AL  +Y+ CG  E A Q  + M      
Sbjct: 447 AYCNGQQYHAQLLKIGFDSSL--SAGN-----ALITMYAKCGVVEEARQVFRTMPCL--- 506

Query: 398 KEKGISWIHIKNEV--HAFGVEDVIHPQKDEIYKLMAEIWEEIKKMGFIPDTESVL 436
               +SW  +   +  H  G E V             +++EE+ K G  PD  ++L
Sbjct: 507 --DSVSWNALIAALGQHGHGAEAV-------------DVYEEMLKKGIRPDRITLL 522

BLAST of Csa5G622560.2 vs. TAIR10
Match: AT1G20230.1 (AT1G20230.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 444.5 bits (1142), Expect = 8.9e-125
Identity = 221/514 (43.00%), Postives = 323/514 (62.84%), Query Frame = 1

Query: 9   PDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSGGVEIARLI 68
           PD  T++S+L +  + E LN+G+ IH Y+++        V +A+I MY KSG   +  +I
Sbjct: 250 PDQVTVSSVLPSVGDSEMLNMGRLIHGYVIKQGLLKDKCVISAMIDMYGKSG--HVYGII 309

Query: 69  VEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDR----DVVAWTAMIVGYVQNG 128
              N+   +      + + G ++ G V  A E+F   +++    +VV+WT++I G  QNG
Sbjct: 310 SLFNQFEMMEAGVCNAYITGLSRNGLVDKALEMFELFKEQTMELNVVSWTSIIAGCAQNG 369

Query: 129 LWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTPSVTN 188
              +ALELFR M   G +PN  T+ +ML    ++  L HG+  H  A++        V +
Sbjct: 370 KDIEALELFREMQVAGVKPNHVTIPSMLPACGNIAALGHGRSTHGFAVRVHLLDNVHVGS 429

Query: 189 ALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLSVGMK 248
           ALI MYAK G IN+++ VF++    K +V W S++   + HG  KE +++FE ++   +K
Sbjct: 430 ALIDMYAKCGRINLSQIVFNMMP-TKNLVCWNSLMNGFSMHGKAKEVMSIFESLMRTRLK 489

Query: 249 PDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQEAYL 308
           PD I++  +LSAC  VGL ++G KY+ MM+E + I+P L HY+CM++L GRAG LQEAY 
Sbjct: 490 PDFISFTSLLSACGQVGLTDEGWKYFKMMSEEYGIKPRLEHYSCMVNLLGRAGKLQEAYD 549

Query: 309 FIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNSGAYLALANVYSACGK 368
            I+ MP EPD+  WG+LL SC++  N DLA++AAE+L  ++P N G Y+ L+N+Y+A G 
Sbjct: 550 LIKEMPFEPDSCVWGALLNSCRLQNNVDLAEIAAEKLFHLEPENPGTYVLLSNIYAAKGM 609

Query: 369 WENAAQTRKLMKDRGVRKEKGISWIHIKNEVHAFGVEDVIHPQKDEIYKLMAEIWEEIKK 428
           W      R  M+  G++K  G SWI +KN V+     D  HPQ D+I + M EI +E++K
Sbjct: 610 WTEVDSIRNKMESLGLKKNPGCSWIQVKNRVYTLLAGDKSHPQIDQITEKMDEISKEMRK 669

Query: 429 MGFIPDTESVLHDLEEEVKEQILKYHSEKLAIAFGLLNTPENTALRIMKNLRVCNDCHSA 488
            G  P+ +  LHD+EE+ +EQ+L  HSEKLA+ FGLLNTP+ T L+++KNLR+C DCH+ 
Sbjct: 670 SGHRPNLDFALHDVEEQEQEQMLWGHSEKLAVVFGLLNTPDGTPLQVIKNLRICGDCHAV 729

Query: 489 IKFISKLVGREIIVRDATRFHHFKDGSCSCRDYW 519
           IKFIS   GREI +RD  RFHHFKDG CSC D+W
Sbjct: 730 IKFISSYAGREIFIRDTNRFHHFKDGICSCGDFW 760


HSP 2 Score: 105.1 bits (261), Expect = 1.3e-22
Identity = 104/476 (21.85%), Postives = 194/476 (40.76%), Query Frame = 1

Query: 7   LKPDNFTLASILSACANLEKLNIGK--------------------QIHAY---------- 66
           L PD+  L ++   CA L    +GK                      H Y          
Sbjct: 112 LIPDSHVLPNLFKVCAELSAFKVGKQIHCVSCVSGLDMDAFVQGSMFHMYMRCGRMGDAR 171

Query: 67  -ILRAETETSGAVGNALISMYAKSGGVEIARLIVEHNRTSNL--NIIAFTSLLDGYTKLG 126
            +    ++      +AL+  YA+ G +E    I+    +S +  NI+++  +L G+ + G
Sbjct: 172 KVFDRMSDKDVVTCSALLCAYARKGCLEEVVRILSEMESSGIEANIVSWNGILSGFNRSG 231

Query: 127 NVKPAREIFNKLRDR----DVVAWTAMIVGYVQNGLWNDALELFRLMVNEGPEPNSYTLA 186
             K A  +F K+       D V  ++++     + + N    +   ++ +G   +   ++
Sbjct: 232 YHKEAVVMFQKIHHLGFCPDQVTVSSVLPSVGDSEMLNMGRLIHGYVIKQGLLKDKCVIS 291

Query: 187 AMLSVSSSLTILEHGKQIHA----SAIKAGESSTPSVTNALIAMYAKTGNINVAKRVFDL 246
           AM+ +        +GK  H     S     E     V NA I   ++ G ++ A  +F+L
Sbjct: 292 AMIDM--------YGKSGHVYGIISLFNQFEMMEAGVCNAYITGLSRNGLVDKALEMFEL 351

Query: 247 ---PNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLSVGMKPDHITYVGVLSACTHVGL 306
                 +  +VSWTS+I   AQ+G   EA+ LF  M   G+KP+H+T   +L AC ++  
Sbjct: 352 FKEQTMELNVVSWTSIIAGCAQNGKDIEALELFREMQVAGVKPNHVTIPSMLPACGNIAA 411

Query: 307 VEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQEAYLFIESMPIEPDNIAWGSLL 366
           +  GR  +     VH ++  +   + +ID+Y + G +  + +    MP + + + W SL+
Sbjct: 412 LGHGRSTHGFAVRVHLLD-NVHVGSALIDMYAKCGRINLSQIVFNMMPTK-NLVCWNSLM 471

Query: 367 ASCKIHKNADLAKVAAERLL--LIDPGNSGAYLALANVYSACGKWENAAQTRKLMKDRGV 426
               +H  A       E L+   + P     +++  ++ SACG+          + D G 
Sbjct: 472 NGFSMHGKAKEVMSIFESLMRTRLKPD----FISFTSLLSACGQVG--------LTDEG- 531

Query: 427 RKEKGISWIHIKNEVHAFGVEDVIHPQKDEIYKL-----MAEIWEEIKKMGFIPDT 432
                  W + K     +G++  +      +  L     + E ++ IK+M F PD+
Sbjct: 532 -------WKYFKMMSEEYGIKPRLEHYSCMVNLLGRAGKLQEAYDLIKEMPFEPDS 557

BLAST of Csa5G622560.2 vs. TAIR10
Match: AT4G02750.1 (AT4G02750.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 437.6 bits (1124), Expect = 1.1e-122
Identity = 205/469 (43.71%), Postives = 311/469 (66.31%), Query Frame = 1

Query: 50  NALISMYAKSGGVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRD 109
           NA+++ Y +   +E+A+ + +     N++   + +++ GY + G +  A+ +F+K+  RD
Sbjct: 316 NAMLAGYVQGERMEMAKELFDVMPCRNVS--TWNTMITGYAQCGKISEAKNLFDKMPKRD 375

Query: 110 VVAWTAMIVGYVQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHA 169
            V+W AMI GY Q+G   +AL LF  M  EG   N  + ++ LS  + +  LE GKQ+H 
Sbjct: 376 PVSWAAMIAGYSQSGHSFEALRLFVQMEREGGRLNRSSFSSALSTCADVVALELGKQLHG 435

Query: 170 SAIKAGESSTPSVTNALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGK 229
             +K G  +   V NAL+ MY K G+I  A  +F    GK +IVSW +MI   ++HG G+
Sbjct: 436 RLVKGGYETGCFVGNALLLMYCKCGSIEEANDLFKEMAGK-DIVSWNTMIAGYSRHGFGE 495

Query: 230 EAINLFERMLSVGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACM 289
            A+  FE M   G+KPD  T V VLSAC+H GLV++GR+Y+  MT+ + + P   HYACM
Sbjct: 496 VALRFFESMKREGLKPDDATMVAVLSACSHTGLVDKGRQYFYTMTQDYGVMPNSQHYACM 555

Query: 290 IDLYGRAGLLQEAYLFIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNS 349
           +DL GRAGLL++A+  +++MP EPD   WG+LL + ++H N +LA+ AA+++  ++P NS
Sbjct: 556 VDLLGRAGLLEDAHNLMKNMPFEPDAAIWGTLLGASRVHGNTELAETAADKIFAMEPENS 615

Query: 350 GAYLALANVYSACGKWENAAQTRKLMKDRGVRKEKGISWIHIKNEVHAFGVEDVIHPQKD 409
           G Y+ L+N+Y++ G+W +  + R  M+D+GV+K  G SWI I+N+ H F V D  HP+KD
Sbjct: 616 GMYVLLSNLYASSGRWGDVGKLRVRMRDKGVKKVPGYSWIEIQNKTHTFSVGDEFHPEKD 675

Query: 410 EIYKLMAEIWEEIKKMGFIPDTESVLHDLEEEVKEQILKYHSEKLAIAFGLLNTPENTAL 469
           EI+  + E+   +KK G++  T  VLHD+EEE KE++++YHSE+LA+A+G++       +
Sbjct: 676 EIFAFLEELDLRMKKAGYVSKTSVVLHDVEEEEKERMVRYHSERLAVAYGIMRVSSGRPI 735

Query: 470 RIMKNLRVCNDCHSAIKFISKLVGREIIVRDATRFHHFKDGSCSCRDYW 519
           R++KNLRVC DCH+AIK+++++ GR II+RD  RFHHFKDGSCSC DYW
Sbjct: 736 RVIKNLRVCEDCHNAIKYMARITGRLIILRDNNRFHHFKDGSCSCGDYW 781


HSP 2 Score: 105.9 bits (263), Expect = 7.5e-23
Identity = 81/289 (28.03%), Postives = 144/289 (49.83%), Query Frame = 1

Query: 51  ALISMYAKSGGVEIARLIVEHNR---TSNL-NIIAFTSLLDGYTKLGNVKPAREIFNKLR 110
           AL+S     GG    + IVE  +   + N+ +++++ +++ GY + G +  AR++F++  
Sbjct: 218 ALVSWNCLLGGFVKKKKIVEARQFFDSMNVRDVVSWNTIITGYAQSGKIDEARQLFDESP 277

Query: 111 DRDVVAWTAMIVGYVQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQ 170
            +DV  WTAM+ GY+QN +  +A ELF  M    PE N  +  AML+       +E  K+
Sbjct: 278 VQDVFTWTAMVSGYIQNRMVEEARELFDKM----PERNEVSWNAMLAGYVQGERMEMAKE 337

Query: 171 IHASAIKAGESSTPSVTNALIAMYAKTGNINVAKRVFD-LPNGKKEIVSWTSMIMALAQH 230
           +             S  N +I  YA+ G I+ AK +FD +P  K++ VSW +MI   +Q 
Sbjct: 338 L----FDVMPCRNVSTWNTMITGYAQCGKISEAKNLFDKMP--KRDPVSWAAMIAGYSQS 397

Query: 231 GLGKEAINLFERMLSVGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSH 290
           G   EA+ LF +M   G + +  ++   LS C  V  +E G++ +  + +          
Sbjct: 398 GHSFEALRLFVQMEREGGRLNRSSFSSALSTCADVVALELGKQLHGRLVKGGYETGCFVG 457

Query: 291 YACMIDLYGRAGLLQEAYLFIESMPIEPDNIAWGSLLASCKIHKNADLA 335
            A ++ +Y + G ++EA    + M    D ++W +++A    H   ++A
Sbjct: 458 NALLL-MYCKCGSIEEANDLFKEM-AGKDIVSWNTMIAGYSRHGFGEVA 494


HSP 3 Score: 98.2 bits (243), Expect = 1.6e-20
Identity = 80/300 (26.67%), Postives = 136/300 (45.33%), Query Frame = 1

Query: 50  NALISMYAKSGGVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRD 109
           N +IS Y ++G  E+AR + +     +L  +++  ++ GY +  N+  ARE+F  + +RD
Sbjct: 99  NGMISGYLRNGEFELARKLFDEMPERDL--VSWNVMIKGYVRNRNLGKARELFEIMPERD 158

Query: 110 VVAWTAMIVGYVQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHA 169
           V +W  M+ GY QNG  +DA  +F  M    PE N  +  A+LS     + +E    +  
Sbjct: 159 VCSWNTMLSGYAQNGCVDDARSVFDRM----PEKNDVSWNALLSAYVQNSKMEEACML-- 218

Query: 170 SAIKAGESSTPSVTNALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGK 229
              K+ E+      N L+  + K   I  A++ FD  N  +++VSW ++I   AQ G   
Sbjct: 219 --FKSRENWALVSWNCLLGGFVKKKKIVEARQFFDSMN-VRDVVSWNTIITGYAQSGKID 278

Query: 230 EAINLFERMLSVGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEI--EPTLSHYA 289
           EA  LF+         D  T+  ++S      +VE+ R+ ++ M E +E+     L+ Y 
Sbjct: 279 EARQLFDE----SPVQDVFTWTAMVSGYIQNRMVEEARELFDKMPERNEVSWNAMLAGYV 338

Query: 290 ------------------------CMIDLYGRAGLLQEAYLFIESMPIEPDNIAWGSLLA 324
                                    MI  Y + G + EA    + MP + D ++W +++A
Sbjct: 339 QGERMEMAKELFDVMPCRNVSTWNTMITGYAQCGKISEAKNLFDKMP-KRDPVSWAAMIA 382


HSP 4 Score: 79.0 bits (193), Expect = 9.9e-15
Identity = 74/339 (21.83%), Postives = 153/339 (45.13%), Query Frame = 1

Query: 50  NALISMYAKSGGVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRD 109
           N  IS Y ++G    A  + +  R    + +++  ++ GY + G  + AR++F+++ +RD
Sbjct: 68  NVAISSYMRTGRCNEALRVFK--RMPRWSSVSYNGMISGYLRNGEFELARKLFDEMPERD 127

Query: 110 VVAWTAMIVGYVQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHA 169
           +V+W  MI GYV+N     A ELF +M    PE +  +   MLS  +    ++  + +  
Sbjct: 128 LVSWNVMIKGYVRNRNLGKARELFEIM----PERDVCSWNTMLSGYAQNGCVDDARSVFD 187

Query: 170 SAIKAGESSTPSVTNALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGK 229
              +  + S     NAL++ Y +   +  A  +F        +VSW  ++    +     
Sbjct: 188 RMPEKNDVS----WNALLSAYVQNSKMEEACMLFK-SRENWALVSWNCLLGGFVKKKKIV 247

Query: 230 EAINLFERMLSVGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACM 289
           EA   F+ M       D +++  +++     G +++ R+ ++  + V ++      +  M
Sbjct: 248 EARQFFDSM----NVRDVVSWNTIITGYAQSGKIDEARQLFD-ESPVQDV----FTWTAM 307

Query: 290 IDLYGRAGLLQEAYLFIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNS 349
           +  Y +  +++EA    + MP E + ++W ++LA    +   +  ++A E   ++   N 
Sbjct: 308 VSGYIQNRMVEEARELFDKMP-ERNEVSWNAMLAG---YVQGERMEMAKELFDVMPCRNV 367

Query: 350 GAYLALANVYSACGKWENAAQTRKLMKDRGVRKEKGISW 389
             +  +   Y+ CGK   A    K + D+ + K   +SW
Sbjct: 368 STWNTMITGYAQCGKISEA----KNLFDK-MPKRDPVSW 377


HSP 5 Score: 78.6 bits (192), Expect = 1.3e-14
Identity = 91/395 (23.04%), Postives = 167/395 (42.28%), Query Frame = 1

Query: 50  NALISMYAKSGGVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRD 109
           N +I+ YA+SG ++ AR + + +   +  +  +T+++ GY +   V+ ARE+F+K+ +R+
Sbjct: 254 NTIITGYAQSGKIDEARQLFDESPVQD--VFTWTAMVSGYIQNRMVEEARELFDKMPERN 313

Query: 110 VVAWTAMIVGYVQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHA 169
            V+W AM+ GYVQ      A ELF +M    P  N  T   M++  +    +   K +  
Sbjct: 314 EVSWNAMLAGYVQGERMEMAKELFDVM----PCRNVSTWNTMITGYAQCGKISEAKNLFD 373

Query: 170 SAIKAGESSTPSVTNALIAMYAKTGNINVAKRVF---DLPNGKKEIVSWTSMIMALAQHG 229
              K      P    A+IA Y+++G+   A R+F   +   G+    S++S +   A   
Sbjct: 374 KMPK----RDPVSWAAMIAGYSQSGHSFEALRLFVQMEREGGRLNRSSFSSALSTCADVV 433

Query: 230 LGKEAINLFERMLSVGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHY 289
             +    L  R++  G +        +L      G +E+    +  M         +  +
Sbjct: 434 ALELGKQLHGRLVKGGYETGCFVGNALLLMYCKCGSIEEANDLFKEMA-----GKDIVSW 493

Query: 290 ACMIDLYGRAGLLQEAYLFIESMP---IEPDNIAWGSLLASCKIHKNADLAKVAAERLLL 349
             MI  Y R G  + A  F ESM    ++PD+    ++L++C      D  +     +  
Sbjct: 494 NTMIAGYSRHGFGEVALRFFESMKREGLKPDDATMVAVLSACSHTGLVDKGRQYFYTMTQ 553

Query: 350 IDPG---NSGAYLALANVYSACGKWENAAQTRKLMKDRGVRKEK-------GISWIHIKN 409
            D G   NS  Y  + ++    G  E+A     LMK+     +        G S +H   
Sbjct: 554 -DYGVMPNSQHYACMVDLLGRAGLLEDA---HNLMKNMPFEPDAAIWGTLLGASRVHGNT 613

Query: 410 EVHAFGVEDV--IHPQKDEIYKLMAEIWEEIKKMG 427
           E+     + +  + P+   +Y L++ ++    + G
Sbjct: 614 ELAETAADKIFAMEPENSGMYVLLSNLYASSGRWG 629


HSP 6 Score: 65.5 bits (158), Expect = 1.1e-10
Identity = 50/215 (23.26%), Postives = 89/215 (41.40%), Query Frame = 1

Query: 13  TLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSGGVEIARLIVEHN 72
           + +S LS CA++  L +GKQ+H  +++   ET   VGNAL+ MY K G +E A  + +  
Sbjct: 411 SFSSALSTCADVVALELGKQLHGRLVKGGYETGCFVGNALLLMYCKCGSIEEANDLFK-- 470

Query: 73  RTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGYVQNGLWNDALEL 132
             +  +I+++ +                               MI GY ++G    AL  
Sbjct: 471 EMAGKDIVSWNT-------------------------------MIAGYSRHGFGEVALRF 530

Query: 133 FRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQ-IHASAIKAGESSTPSVTNALIAMYA 192
           F  M  EG +P+  T+ A+LS  S   +++ G+Q  +      G          ++ +  
Sbjct: 531 FESMKREGLKPDDATMVAVLSACSHTGLVDKGRQYFYTMTQDYGVMPNSQHYACMVDLLG 590

Query: 193 KTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHG 227
           + G +  A  +      + +   W +++ A   HG
Sbjct: 591 RAGLLEDAHNLMKNMPFEPDAAIWGTLLGASRVHG 592

BLAST of Csa5G622560.2 vs. TAIR10
Match: AT5G48910.1 (AT5G48910.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 434.1 bits (1115), Expect = 1.2e-121
Identity = 224/531 (42.18%), Postives = 330/531 (62.15%), Query Frame = 1

Query: 1   MLNEPSLKPDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSG 60
           M+++  ++P+ FT  S+L ACA   K+  GKQIH   L+        V + L+ MY   G
Sbjct: 118 MMSDEFVEPNRFTFPSVLKACAKTGKIQEGKQIHGLALKYGFGGDEFVMSNLVRMYVMCG 177

Query: 61  GVEIARLIVEHN------------RTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDR 120
            ++ AR++   N            R  +  I+ +  ++DGY +LG+ K AR +F+K+R R
Sbjct: 178 FMKDARVLFYKNIIEKDMVVMTDRRKRDGEIVLWNVMIDGYMRLGDCKAARMLFDKMRQR 237

Query: 121 DVVAWTAMIVGYVQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIH 180
            VV+W  MI GY  NG + DA+E+FR M      PN  TL ++L   S L  LE G+ +H
Sbjct: 238 SVVSWNTMISGYSLNGFFKDAVEVFREMKKGDIRPNYVTLVSVLPAISRLGSLELGEWLH 297

Query: 181 ASAIKAGESSTPSVTNALIAMYAKTGNINVAKRVFD-LPNGKKEIVSWTSMIMALAQHGL 240
             A  +G      + +ALI MY+K G I  A  VF+ LP  ++ +++W++MI   A HG 
Sbjct: 298 LYAEDSGIRIDDVLGSALIDMYSKCGIIEKAIHVFERLP--RENVITWSAMINGFAIHGQ 357

Query: 241 GKEAINLFERMLSVGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYA 300
             +AI+ F +M   G++P  + Y+ +L+AC+H GLVE+GR+Y++ M  V  +EP + HY 
Sbjct: 358 AGDAIDCFCKMRQAGVRPSDVAYINLLTACSHGGLVEEGRRYFSQMVSVDGLEPRIEHYG 417

Query: 301 CMIDLYGRAGLLQEAYLFIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPG 360
           CM+DL GR+GLL EA  FI +MPI+PD++ W +LL +C++  N ++ K  A  L+ + P 
Sbjct: 418 CMVDLLGRSGLLDEAEEFILNMPIKPDDVIWKALLGACRMQGNVEMGKRVANILMDMVPH 477

Query: 361 NSGAYLALANVYSACGKWENAAQTRKLMKDRGVRKEKGISWIHIKNEVHAFGVEDVIHPQ 420
           +SGAY+AL+N+Y++ G W   ++ R  MK++ +RK+ G S I I   +H F VED  HP+
Sbjct: 478 DSGAYVALSNMYASQGNWSEVSEMRLRMKEKDIRKDPGCSLIDIDGVLHEFVVEDDSHPK 537

Query: 421 KDEIYKLMAEIWEEIKKMGFIPDTESVLHDLEEEVKEQILKYHSEKLAIAFGLLNTPENT 480
             EI  ++ EI ++++  G+ P T  VL +LEEE KE +L YHSEK+A AFGL++T    
Sbjct: 538 AKEINSMLVEISDKLRLAGYRPITTQVLLNLEEEDKENVLHYHSEKIATAFGLISTSPGK 597

Query: 481 ALRIMKNLRVCNDCHSAIKFISKLVGREIIVRDATRFHHFKDGSCSCRDYW 519
            +RI+KNLR+C DCHS+IK ISK+  R+I VRD  RFHHF+DGSCSC DYW
Sbjct: 598 PIRIVKNLRICEDCHSSIKLISKVYKRKITVRDRKRFHHFQDGSCSCMDYW 646

BLAST of Csa5G622560.2 vs. NCBI nr
Match: gi|449449306|ref|XP_004142406.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g22070 [Cucumis sativus])

HSP 1 Score: 1036.2 bits (2678), Expect = 2.0e-299
Identity = 518/518 (100.00%), Postives = 518/518 (100.00%), Query Frame = 1

Query: 1   MLNEPSLKPDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSG 60
           MLNEPSLKPDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSG
Sbjct: 265 MLNEPSLKPDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSG 324

Query: 61  GVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGY 120
           GVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGY
Sbjct: 325 GVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGY 384

Query: 121 VQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTP 180
           VQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTP
Sbjct: 385 VQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTP 444

Query: 181 SVTNALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLS 240
           SVTNALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLS
Sbjct: 445 SVTNALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLS 504

Query: 241 VGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQ 300
           VGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQ
Sbjct: 505 VGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQ 564

Query: 301 EAYLFIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNSGAYLALANVYS 360
           EAYLFIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNSGAYLALANVYS
Sbjct: 565 EAYLFIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNSGAYLALANVYS 624

Query: 361 ACGKWENAAQTRKLMKDRGVRKEKGISWIHIKNEVHAFGVEDVIHPQKDEIYKLMAEIWE 420
           ACGKWENAAQTRKLMKDRGVRKEKGISWIHIKNEVHAFGVEDVIHPQKDEIYKLMAEIWE
Sbjct: 625 ACGKWENAAQTRKLMKDRGVRKEKGISWIHIKNEVHAFGVEDVIHPQKDEIYKLMAEIWE 684

Query: 421 EIKKMGFIPDTESVLHDLEEEVKEQILKYHSEKLAIAFGLLNTPENTALRIMKNLRVCND 480
           EIKKMGFIPDTESVLHDLEEEVKEQILKYHSEKLAIAFGLLNTPENTALRIMKNLRVCND
Sbjct: 685 EIKKMGFIPDTESVLHDLEEEVKEQILKYHSEKLAIAFGLLNTPENTALRIMKNLRVCND 744

Query: 481 CHSAIKFISKLVGREIIVRDATRFHHFKDGSCSCRDYW 519
           CHSAIKFISKLVGREIIVRDATRFHHFKDGSCSCRDYW
Sbjct: 745 CHSAIKFISKLVGREIIVRDATRFHHFKDGSCSCRDYW 782

BLAST of Csa5G622560.2 vs. NCBI nr
Match: gi|449449306|ref|XP_004142406.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g22070 [Cucumis sativus])

HSP 1 Score: 144.4 bits (363), Expect = 5.4e-31
Identity = 89/294 (30.27%), Postives = 164/294 (55.78%), Query Frame = 1

Query: 9   PDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSGGVEIARLI 68
           P  FT++++LS+CA  + L+IG++IH+++++    +   V  +L++MYAK G   IA+++
Sbjct: 140 PSQFTVSNVLSSCAANQTLDIGRKIHSFVVKLGLGSCVPVATSLLNMYAKCGDPVIAKVV 199

Query: 69  VEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGYVQNGLWND 128
            +  R +  NI  + +L+  Y + G  + A   F K+ DRD+V+W +MI GY Q G   +
Sbjct: 200 FD--RMTVKNISTWNALISLYMQSGQFELAASQFEKMPDRDIVSWNSMISGYSQQGYNLE 259

Query: 129 ALELFRLMVNE-GPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTPSVTNALI 188
           AL +F  M+NE   +P+++TLA++LS  ++L  L  GKQIHA  ++A   ++ +V NALI
Sbjct: 260 ALVIFSKMLNEPSLKPDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALI 319

Query: 189 AMYAKTGNINVAKRVFD-LPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLSVGMKPD 248
           +MYAK+G + +A+ + +        I+++TS++    + G  K A  +F ++       D
Sbjct: 320 SMYAKSGGVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKL----RDRD 379

Query: 249 HITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQ 301
            + +  ++      GL     + + +M      EP     A M+ +     +L+
Sbjct: 380 VVAWTAMIVGYVQNGLWNDALELFRLMVN-EGPEPNSYTLAAMLSVSSSLTILE 426


HSP 2 Score: 122.9 bits (307), Expect = 1.7e-24
Identity = 91/369 (24.66%), Postives = 179/369 (48.51%), Query Frame = 1

Query: 15  ASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSGGVEIARLIVEHNRT 74
           A IL     ++    G+ +H  I++        + N L++ YAK+G +  A  + +    
Sbjct: 14  AHILQTSVRIKDPFAGRSVHCQIIKKGLHLGVYLMNNLMTFYAKTGSLRFAHHVFDEMPL 73

Query: 75  SNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGYVQNGLWNDALELFR 134
            +    ++ +L+ GY K GN + +R +  ++ D D V+WTA+IVGY Q GL+++A+ +F 
Sbjct: 74  KST--FSWNTLISGYAKQGNFEVSRRLLYEMPDCDPVSWTAIIVGYNQFGLFDNAIWMFA 133

Query: 135 LMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTPSVTNALIAMYAKTG 194
            M++E   P+ +T++ +LS  ++   L+ G++IH+  +K G  S   V  +L+ MYAK G
Sbjct: 134 KMISERVPPSQFTVSNVLSSCAANQTLDIGRKIHSFVVKLGLGSCVPVATSLLNMYAKCG 193

Query: 195 NINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLSVGMKPDHITYVGVL 254
           +  +AK VFD     K I +W ++I    Q G  + A + FE+M       D +++  ++
Sbjct: 194 DPVIAKVVFDRMT-VKNISTWNALISLYMQSGQFELAASQFEKM----PDRDIVSWNSMI 253

Query: 255 SACTHVGLVEQGRKYYNMMTEVHEIEP---TLSHYACMIDLYGRAGLLQEAYLFIESMPI 314
           S  +  G   +    ++ M     ++P   TL+          +  + ++ + +I     
Sbjct: 254 SGYSQQGYNLEALVIFSKMLNEPSLKPDNFTLASILSACANLEKLNIGKQIHAYILRAET 313

Query: 315 EPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNSGAYLALANVYSACGKWENAAQT 374
           E       +L++        ++A++  E     +  N  A+ +L + Y+  G  + A + 
Sbjct: 314 ETSGAVGNALISMYAKSGGVEIARLIVEHNRTSNL-NIIAFTSLLDGYTKLGNVKPAREI 373

Query: 375 RKLMKDRGV 381
              ++DR V
Sbjct: 374 FNKLRDRDV 374


HSP 3 Score: 1036.2 bits (2678), Expect = 2.0e-299
Identity = 518/518 (100.00%), Postives = 518/518 (100.00%), Query Frame = 1

Query: 1   MLNEPSLKPDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSG 60
           MLNEPSLKPDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSG
Sbjct: 205 MLNEPSLKPDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSG 264

Query: 61  GVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGY 120
           GVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGY
Sbjct: 265 GVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGY 324

Query: 121 VQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTP 180
           VQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTP
Sbjct: 325 VQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTP 384

Query: 181 SVTNALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLS 240
           SVTNALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLS
Sbjct: 385 SVTNALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLS 444

Query: 241 VGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQ 300
           VGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQ
Sbjct: 445 VGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQ 504

Query: 301 EAYLFIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNSGAYLALANVYS 360
           EAYLFIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNSGAYLALANVYS
Sbjct: 505 EAYLFIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNSGAYLALANVYS 564

Query: 361 ACGKWENAAQTRKLMKDRGVRKEKGISWIHIKNEVHAFGVEDVIHPQKDEIYKLMAEIWE 420
           ACGKWENAAQTRKLMKDRGVRKEKGISWIHIKNEVHAFGVEDVIHPQKDEIYKLMAEIWE
Sbjct: 565 ACGKWENAAQTRKLMKDRGVRKEKGISWIHIKNEVHAFGVEDVIHPQKDEIYKLMAEIWE 624

Query: 421 EIKKMGFIPDTESVLHDLEEEVKEQILKYHSEKLAIAFGLLNTPENTALRIMKNLRVCND 480
           EIKKMGFIPDTESVLHDLEEEVKEQILKYHSEKLAIAFGLLNTPENTALRIMKNLRVCND
Sbjct: 625 EIKKMGFIPDTESVLHDLEEEVKEQILKYHSEKLAIAFGLLNTPENTALRIMKNLRVCND 684

Query: 481 CHSAIKFISKLVGREIIVRDATRFHHFKDGSCSCRDYW 519
           CHSAIKFISKLVGREIIVRDATRFHHFKDGSCSCRDYW
Sbjct: 685 CHSAIKFISKLVGREIIVRDATRFHHFKDGSCSCRDYW 722

BLAST of Csa5G622560.2 vs. NCBI nr
Match: gi|700197064|gb|KGN52241.1| (hypothetical protein Csa_5G622560 [Cucumis sativus])

HSP 1 Score: 110.5 bits (275), Expect = 8.7e-21
Identity = 79/315 (25.08%), Postives = 160/315 (50.79%), Query Frame = 1

Query: 15  ASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSGGVEIARLIVEHNRT 74
           A IL     ++    G+ +H  I++        + N L++ YAK+G +  A  + +    
Sbjct: 14  AHILQTSVRIKDPFAGRSVHCQIIKKGLHLGVYLMNNLMTFYAKTGSLRFAHHVFDEMPL 73

Query: 75  SNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGYVQNGLWNDALELFR 134
            +    ++ +L+ GY K GN + +R +  ++ D D V+WTA+IVGY Q GL+++A+ +F 
Sbjct: 74  KST--FSWNTLISGYAKQGNFEVSRRLLYEMPDCDPVSWTAIIVGYNQFGLFDNAIWMFA 133

Query: 135 LMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTPSVTNALIAMYAKTG 194
            M++E   P+ +T++ +LS  ++   L+ G++IH+  +K G  S   V  +L+ MYAK G
Sbjct: 134 KMISERVPPSQFTVSNVLSSCAANQTLDIGRKIHSFVVKLGLGSCVPVATSLLNMYAKCG 193

Query: 195 NINVAKRVFDL------PNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLSVGMKPDHI 254
           +  +AK VFD       P+ K +  +  S++ A A      E +N+ +++ +  ++ +  
Sbjct: 194 DPVIAKVVFDRMTMLNEPSLKPDNFTLASILSACA----NLEKLNIGKQIHAYILRAETE 253

Query: 255 TYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEP-TLSHYACMIDLYGRAGLLQEAYLFIE 314
           T   V +A   +     G +   ++ E +      +  +  ++D Y + G ++ A     
Sbjct: 254 TSGAVGNALISMYAKSGGVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFN 313

Query: 315 SMPIEPDNIAWGSLL 323
            +  + D +AW +++
Sbjct: 314 KLR-DRDVVAWTAMI 321


HSP 2 Score: 61.2 bits (147), Expect = 6.0e-06
Identity = 73/332 (21.99%), Postives = 143/332 (43.07%), Query Frame = 1

Query: 9   PDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSGGVEIARLI 68
           P  FT++++LS+CA  + L+IG++IH+++++    +   V  +L++MYAK G   IA+++
Sbjct: 140 PSQFTVSNVLSSCAANQTLDIGRKIHSFVVKLGLGSCVPVATSLLNMYAKCGDPVIAKVV 199

Query: 69  VEHN--------RTSNLNIIAFTSLLDGYTKLGNVKPARE-IFNKLRDRDVVAWTAMIVG 128
            +          +  N  + +  S      KL   K     I     +       A+I  
Sbjct: 200 FDRMTMLNEPSLKPDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISM 259

Query: 129 YVQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESST 188
           Y ++G     +E+ RL+V E    ++  + A  S+    T L + K       K  +   
Sbjct: 260 YAKSG----GVEIARLIV-EHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDV 319

Query: 189 PSVTNALIAMYAKTGNINVAKRVFDL-PNGKKEIVSWT--SMIMALAQHGLGKEAINLFE 248
            + T A+I  Y + G  N A  +F L  N   E  S+T  +M+   +   + +    +  
Sbjct: 320 VAWT-AMIVGYVQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHA 379

Query: 249 RMLSVGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRA 308
             +  G          +++     G +   ++ +++     EI      +  MI    + 
Sbjct: 380 SAIKAGESSTPSVTNALIAMYAKTGNINVAKRVFDLPNGKKEI----VSWTSMIMALAQH 439

Query: 309 GLLQEAYLFIE---SMPIEPDNIAWGSLLASC 326
           GL +EA    E   S+ ++PD+I +  +L++C
Sbjct: 440 GLGKEAINLFERMLSVGMKPDHITYVGVLSAC 461


HSP 3 Score: 994.6 bits (2570), Expect = 6.6e-287
Identity = 494/518 (95.37%), Postives = 505/518 (97.49%), Query Frame = 1

Query: 1   MLNEPSLKPDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSG 60
           MLNEPSLKPDNFTLASILSACANLEKLNIGKQIHAYILR E ETSGAVGNALISMYAKSG
Sbjct: 265 MLNEPSLKPDNFTLASILSACANLEKLNIGKQIHAYILRTEAETSGAVGNALISMYAKSG 324

Query: 61  GVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGY 120
           GVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRD DV+AWTAMIVGY
Sbjct: 325 GVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDCDVIAWTAMIVGY 384

Query: 121 VQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTP 180
           VQNGLWNDAL+LFRLMVNEGPEPNSYTLAAMLSVSSSL  LEHGKQIHASAIKAGESSTP
Sbjct: 385 VQNGLWNDALDLFRLMVNEGPEPNSYTLAAMLSVSSSLATLEHGKQIHASAIKAGESSTP 444

Query: 181 SVTNALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLS 240
           SVTNALI MYAKTGNI+VAKRVFDL +GKKEIVSWTSMIMALAQHGLGKEAINLFERM S
Sbjct: 445 SVTNALITMYAKTGNISVAKRVFDLTSGKKEIVSWTSMIMALAQHGLGKEAINLFERMRS 504

Query: 241 VGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQ 300
           +GMKPDHITYVGVLSACTHVG VEQGRKYY MMTEVHEIEPTLSHYACMIDLYGRAGLLQ
Sbjct: 505 LGMKPDHITYVGVLSACTHVGFVEQGRKYYKMMTEVHEIEPTLSHYACMIDLYGRAGLLQ 564

Query: 301 EAYLFIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNSGAYLALANVYS 360
           EAY FIESMPIEPDNIAWGSLLASC++HKNADLAKVAAERLLLIDPGNSGAYLALANVYS
Sbjct: 565 EAYQFIESMPIEPDNIAWGSLLASCRVHKNADLAKVAAERLLLIDPGNSGAYLALANVYS 624

Query: 361 ACGKWENAAQTRKLMKDRGVRKEKGISWIHIKNEVHAFGVEDVIHPQKDEIYKLMAEIWE 420
           ACGKWE+AA+TRKLMKDRGVRKEKG SWIHIKN+VHAFGVEDVIHPQKDEIYKLMAEIWE
Sbjct: 625 ACGKWESAAKTRKLMKDRGVRKEKGFSWIHIKNKVHAFGVEDVIHPQKDEIYKLMAEIWE 684

Query: 421 EIKKMGFIPDTESVLHDLEEEVKEQILKYHSEKLAIAFGLLNTPENTALRIMKNLRVCND 480
           EIKKMGFIPDTESVLHDLEEEVKEQILK+HSEKLAIAFGLLNTPENT LRIMKNLRVCND
Sbjct: 685 EIKKMGFIPDTESVLHDLEEEVKEQILKHHSEKLAIAFGLLNTPENTVLRIMKNLRVCND 744

Query: 481 CHSAIKFISKLVGREIIVRDATRFHHFKDGSCSCRDYW 519
           CHSAIKFISKLVGREIIVRDATRFHHFKDGSCSCRDYW
Sbjct: 745 CHSAIKFISKLVGREIIVRDATRFHHFKDGSCSCRDYW 782

BLAST of Csa5G622560.2 vs. NCBI nr
Match: gi|659091956|ref|XP_008446823.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g22070 [Cucumis melo])

HSP 1 Score: 142.9 bits (359), Expect = 1.6e-30
Identity = 89/294 (30.27%), Postives = 161/294 (54.76%), Query Frame = 1

Query: 9   PDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSGGVEIARLI 68
           P  FT++++LS+CA  + L+IG++IH+++++    +   V  +L++MYAK G   IA+++
Sbjct: 140 PSQFTVSNVLSSCAANQALDIGRKIHSFVVKLGLGSCAPVATSLLNMYAKCGDPVIAKVV 199

Query: 69  VEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGYVQNGLWND 128
            +  R +  NI  + +L+  Y + G  + A   F K+ DRD+V+W +MI GY Q G   +
Sbjct: 200 FD--RMTVKNISTWNALISLYMQSGQFELAASQFEKMPDRDIVSWNSMISGYSQQGNNLE 259

Query: 129 ALELFRLMVNE-GPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTPSVTNALI 188
           AL +F  M+NE   +P+++TLA++LS  ++L  L  GKQIHA  ++    ++ +V NALI
Sbjct: 260 ALVIFSKMLNEPSLKPDNFTLASILSACANLEKLNIGKQIHAYILRTEAETSGAVGNALI 319

Query: 189 AMYAKTGNINVAKRVFD-LPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLSVGMKPD 248
           +MYAK+G + +A+ + +        I+++TS++    + G  K A  +F ++       D
Sbjct: 320 SMYAKSGGVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDC----D 379

Query: 249 HITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQ 301
            I +  ++      GL       + +M      EP     A M+ +      L+
Sbjct: 380 VIAWTAMIVGYVQNGLWNDALDLFRLMVN-EGPEPNSYTLAAMLSVSSSLATLE 426


HSP 2 Score: 120.6 bits (301), Expect = 8.4e-24
Identity = 70/224 (31.25%), Postives = 125/224 (55.80%), Query Frame = 1

Query: 15  ASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSGGVEIARLIVEHNRT 74
           A IL     ++    G+ +H+ I++        + N L++ YAK+G +  A  + +    
Sbjct: 14  AHILQTSVRIKDPFAGRSVHSQIIKKGLHLGVYLMNNLMTFYAKTGSLSFAHHVFDEMPL 73

Query: 75  SNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGYVQNGLWNDALELFR 134
            +    ++ +L+ GY K GN + +R +  ++ D D V+WTA+IVGY Q GL+++A+ +F 
Sbjct: 74  KST--FSWNTLISGYAKQGNFEVSRRLLYEMPDCDPVSWTAIIVGYNQFGLFDNAIWMFA 133

Query: 135 LMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTPSVTNALIAMYAKTG 194
            M++E   P+ +T++ +LS  ++   L+ G++IH+  +K G  S   V  +L+ MYAK G
Sbjct: 134 KMISERVPPSQFTVSNVLSSCAANQALDIGRKIHSFVVKLGLGSCAPVATSLLNMYAKCG 193

Query: 195 NINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERM 239
           +  +AK VFD     K I +W ++I    Q G  + A + FE+M
Sbjct: 194 DPVIAKVVFDRMT-VKNISTWNALISLYMQSGQFELAASQFEKM 234


HSP 3 Score: 812.0 bits (2096), Expect = 6.0e-232
Identity = 390/518 (75.29%), Postives = 460/518 (88.80%), Query Frame = 1

Query: 1   MLNEPSLKPDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSG 60
           ML + SLKPD F+LAS LSACANLEKL+ GKQIH YI+R   + SGAVGNALISMYAKSG
Sbjct: 268 MLKDTSLKPDRFSLASALSACANLEKLSFGKQIHGYIVRTMFDASGAVGNALISMYAKSG 327

Query: 61  GVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGY 120
           GVEIAR I+E +  S+L++IAFT+LLDGY KLG++ PAR+IFN L+D DVVAWTAMIVGY
Sbjct: 328 GVEIARRIIEQSGISDLDVIAFTALLDGYVKLGDITPARQIFNSLKDPDVVAWTAMIVGY 387

Query: 121 VQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTP 180
           VQNGL NDA+E+F+ MV+EGP PNS+TLAAMLS SSS+T L HGKQIHASAI++GE+ +P
Sbjct: 388 VQNGLNNDAIEVFKTMVSEGPRPNSFTLAAMLSASSSVTSLNHGKQIHASAIRSGEALSP 447

Query: 181 SVTNALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLS 240
           SV NAL  MYAK G+IN A++VF+L    ++ VSWTSMIMALAQHGLG+EAI LFE+ML+
Sbjct: 448 SVGNALTTMYAKAGSINGARKVFNLLRQNRDTVSWTSMIMALAQHGLGEEAIELFEQMLA 507

Query: 241 VGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQ 300
           +G+KPDHITYVGVLSACTH GLVEQGR Y+++M  VH+I+PTLSHYACM+DL+GRAGLLQ
Sbjct: 508 LGIKPDHITYVGVLSACTHGGLVEQGRNYFDLMKNVHKIDPTLSHYACMVDLFGRAGLLQ 567

Query: 301 EAYLFIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNSGAYLALANVYS 360
           EAY F+E+MP EPD IAWGSLL+SCK++KN DLAKVAAERLLLI+P NSGAY ALANVYS
Sbjct: 568 EAYKFVENMPTEPDVIAWGSLLSSCKVYKNVDLAKVAAERLLLIEPNNSGAYSALANVYS 627

Query: 361 ACGKWENAAQTRKLMKDRGVRKEKGISWIHIKNEVHAFGVEDVIHPQKDEIYKLMAEIWE 420
           +CGKW++AA+ RKLMK RGV+KE+G+SW+ I+N+ H FGVED +HPQKDEIYK+M +IW+
Sbjct: 628 SCGKWDDAAKIRKLMKARGVKKEQGLSWVQIQNKTHVFGVEDGLHPQKDEIYKMMDKIWK 687

Query: 421 EIKKMGFIPDTESVLHDLEEEVKEQILKYHSEKLAIAFGLLNTPENTALRIMKNLRVCND 480
           EIKKMGF PDTESVLHDLE EVK+QIL+YHSEKLAIAFG+++TPENT LRIMKNLRVCND
Sbjct: 688 EIKKMGFAPDTESVLHDLEVEVKDQILRYHSEKLAIAFGIISTPENTTLRIMKNLRVCND 747

Query: 481 CHSAIKFISKLVGREIIVRDATRFHHFKDGSCSCRDYW 519
           CH+AIKFISKLV REIIVRDATRFHHFKDG CSC+DYW
Sbjct: 748 CHNAIKFISKLVDREIIVRDATRFHHFKDGFCSCKDYW 785

BLAST of Csa5G622560.2 vs. NCBI nr
Match: gi|743838060|ref|XP_011025626.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g22070-like [Populus euphratica])

HSP 1 Score: 131.7 bits (330), Expect = 3.6e-27
Identity = 74/260 (28.46%), Postives = 148/260 (56.92%), Query Frame = 1

Query: 9   PDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSGGVEIARLI 68
           P  FTL ++L++CA      IGK++H+++++        V N+L++MYAK+G +++A+++
Sbjct: 143 PTQFTLTNVLASCAATGSRGIGKKVHSFVVKLGLHACVPVANSLLNMYAKTGDLKMAKVV 202

Query: 69  VEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGYVQNGLWND 128
            +  +  N +  ++ +++  +   G V  A   F  L +RD+V+W +MI G  Q+G  N+
Sbjct: 203 FDRMKLRNTS--SWNAMISLHMNCGRVDLALAQFELLSERDIVSWNSMIAGCNQHGFDNE 262

Query: 129 ALELFRLMVNE-GPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTPSVTNALI 188
           AL+ F  M+ +   +P+ ++LA+ LS  ++L  L  GKQIH   ++    ++ +V NALI
Sbjct: 263 ALQFFSSMLKDTSLKPDRFSLASALSACANLEKLSFGKQIHGYIVRTMFDASGAVGNALI 322

Query: 189 AMYAKTGNINVAKRVFDLPN-GKKEIVSWTSMIMALAQHGLGKEAINLFERMLSVGMKPD 248
           +MYAK+G + +A+R+ +       +++++T+++    + G    A  +F  +      PD
Sbjct: 323 SMYAKSGGVEIARRIIEQSGISDLDVIAFTALLDGYVKLGDITPARQIFNSL----KDPD 382

Query: 249 HITYVGVLSACTHVGLVEQG 267
            + +  ++     VG V+ G
Sbjct: 383 VVAWTAMI-----VGYVQNG 391


HSP 2 Score: 117.1 bits (292), Expect = 9.3e-23
Identity = 80/284 (28.17%), Postives = 149/284 (52.46%), Query Frame = 1

Query: 8   KPDNFTLASILSACANLEKLNI-------GKQIHAYILRAETETSGAVGNALISMYAKSG 67
           KP  F+  S   +CA+L + ++       GK IHA +++     S  + N L+++YAK+G
Sbjct: 5   KPPYFSCHS--ESCAHLLQTSLKSRDPLKGKSIHAQMVKLGLTFSVYLMNNLMNLYAKTG 64

Query: 68  GVEIARLIVEHNRTSNLNI---IAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMI 127
                 L+  H+    + +    ++ ++L GY K G ++ A ++F+ +  RD V+WT +I
Sbjct: 65  F-----LLDAHDLFKEMPVKTTFSWNTILSGYAKQGKLEKAHQVFDLIPVRDSVSWTTII 124

Query: 128 VGYVQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGES 187
           VGY Q G + DA+++F  MV +   P  +TL  +L+  ++      GK++H+  +K G  
Sbjct: 125 VGYNQMGRFEDAIKIFVDMVKDKVLPTQFTLTNVLASCAATGSRGIGKKVHSFVVKLGLH 184

Query: 188 STPSVTNALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFER 247
           +   V N+L+ MYAKTG++ +AK VFD     +   SW +MI      G    A+  FE 
Sbjct: 185 ACVPVANSLLNMYAKTGDLKMAKVVFDRMK-LRNTSSWNAMISLHMNCGRVDLALAQFEL 244

Query: 248 MLSVGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEP 282
           +     + D +++  +++ C   G   +  ++++ M +   ++P
Sbjct: 245 L----SERDIVSWNSMIAGCNQHGFDNEALQFFSSMLKDTSLKP 276


HSP 3 Score: 60.8 bits (146), Expect = 7.9e-06
Identity = 41/163 (25.15%), Postives = 84/163 (51.53%), Query Frame = 1

Query: 184 NALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLSVGM 243
           N +++ YAK G +  A +VFDL    ++ VSWT++I+   Q G  ++AI +F  M+   +
Sbjct: 83  NTILSGYAKQGKLEKAHQVFDLIP-VRDSVSWTTIIVGYNQMGRFEDAIKIFVDMVKDKV 142

Query: 244 KPDHITYVGVLSACTHVGLVEQGRKYYNMMTE--VHEIEPTLSHYACMIDLYGRAGLLQE 303
            P   T   VL++C   G    G+K ++ + +  +H   P  +    ++++Y + G L+ 
Sbjct: 143 LPTQFTLTNVLASCAATGSRGIGKKVHSFVVKLGLHACVPVAN---SLLNMYAKTGDLKM 202

Query: 304 AYLFIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLI 345
           A +  + M +  +  +W ++++   +H N     +A  +  L+
Sbjct: 203 AKVVFDRMKLR-NTSSWNAMIS---LHMNCGRVDLALAQFELL 237


HSP 4 Score: 55.1 bits (131), Expect = 4.3e-04
Identity = 53/228 (23.25%), Postives = 109/228 (47.81%), Query Frame = 1

Query: 164 GKQIHASAIKAGESSTPSVTNALIAMYAKTGNINVAKRVF-DLPNGKKEIVSWTSMIMAL 223
           GK IHA  +K G + +  + N L+ +YAKTG +  A  +F ++P   K   SW +++   
Sbjct: 32  GKSIHAQMVKLGLTFSVYLMNNLMNLYAKTGFLLDAHDLFKEMP--VKTTFSWNTILSGY 91

Query: 224 AQHGLGKEAINLFERMLSVGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMT--EVHEIE 283
           A+ G  ++A  +F+ ++ V    D +++  ++     +G  E   K +  M   +V   +
Sbjct: 92  AKQGKLEKAHQVFD-LIPV---RDSVSWTTIIVGYNQMGRFEDAIKIFVDMVKDKVLPTQ 151

Query: 284 PTLSHYACMIDLYGRAGLLQEAYLFIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAER 343
            TL++        G  G+ ++ + F+  + +        SLL       +  +AKV  +R
Sbjct: 152 FTLTNVLASCAATGSRGIGKKVHSFVVKLGLHACVPVANSLLNMYAKTGDLKMAKVVFDR 211

Query: 344 LLLIDPGNSGAYLALANVYSACGKWENAAQTRKLMKDRGVRKEKGISW 389
           + L    N+ ++ A+ +++  CG+ + A    +L+ +R +     +SW
Sbjct: 212 MKL---RNTSSWNAMISLHMNCGRVDLALAQFELLSERDI-----VSW 245


HSP 5 Score: 811.6 bits (2095), Expect = 7.9e-232
Identity = 389/518 (75.10%), Postives = 462/518 (89.19%), Query Frame = 1

Query: 1   MLNEPSLKPDNFTLASILSACANLEKLNIGKQIHAYILRAETETSGAVGNALISMYAKSG 60
           +L + SLKPD F+LAS LSACANLEKL+ GKQIH YI+R   + SGAVGNALISMYAKSG
Sbjct: 268 ILKDTSLKPDRFSLASALSACANLEKLSFGKQIHGYIVRTMFDASGAVGNALISMYAKSG 327

Query: 61  GVEIARLIVEHNRTSNLNIIAFTSLLDGYTKLGNVKPAREIFNKLRDRDVVAWTAMIVGY 120
           GVEIAR I+E +  S+L++IAFT+LL+GY KLG++ PAR+IFN L+D DVVAWTAMIVGY
Sbjct: 328 GVEIARRIIEQSGISDLDVIAFTALLNGYVKLGDITPARQIFNSLKDPDVVAWTAMIVGY 387

Query: 121 VQNGLWNDALELFRLMVNEGPEPNSYTLAAMLSVSSSLTILEHGKQIHASAIKAGESSTP 180
           VQNGL NDA+E+F+ MV+EGP PNS+TLAAMLS SSS+T L HGKQIHASAI++GE+ +P
Sbjct: 388 VQNGLNNDAIEVFKTMVSEGPRPNSFTLAAMLSASSSVTSLNHGKQIHASAIRSGEALSP 447

Query: 181 SVTNALIAMYAKTGNINVAKRVFDLPNGKKEIVSWTSMIMALAQHGLGKEAINLFERMLS 240
           SV NAL  MYAK G+IN A++VF+L    ++ VSWTSMIMALAQHGLG+EAI LFE+ML+
Sbjct: 448 SVGNALTTMYAKAGSINGARKVFNLLRQNRDTVSWTSMIMALAQHGLGEEAIELFEQMLT 507

Query: 241 VGMKPDHITYVGVLSACTHVGLVEQGRKYYNMMTEVHEIEPTLSHYACMIDLYGRAGLLQ 300
           +G+KPDHITYVGVLSACTH GLVEQGR Y+++M  VH+I+PTLSHYACM+DL+GRAGLLQ
Sbjct: 508 LGIKPDHITYVGVLSACTHGGLVEQGRSYFDLMKNVHKIDPTLSHYACMVDLFGRAGLLQ 567

Query: 301 EAYLFIESMPIEPDNIAWGSLLASCKIHKNADLAKVAAERLLLIDPGNSGAYLALANVYS 360
           EAY F+E+MP+EPD IAWGSLL+SCK++KN DLAKVAAERLLLI+P NSGAY ALANVYS
Sbjct: 568 EAYKFVENMPMEPDVIAWGSLLSSCKVYKNVDLAKVAAERLLLIEPNNSGAYSALANVYS 627

Query: 361 ACGKWENAAQTRKLMKDRGVRKEKGISWIHIKNEVHAFGVEDVIHPQKDEIYKLMAEIWE 420
           +CGKW++AA+ RKLMK RGV+KE+G+SW+ I+N+ H FGVED +HPQKDEIYK+M +IW+
Sbjct: 628 SCGKWDDAAKIRKLMKARGVKKEQGLSWVQIQNKTHVFGVEDGLHPQKDEIYKMMDKIWK 687

Query: 421 EIKKMGFIPDTESVLHDLEEEVKEQILKYHSEKLAIAFGLLNTPENTALRIMKNLRVCND 480
           EIKKMGF PDTESVLHDLE EVK+QIL+YHSEKLAIAFG+++TPENT LRIMKNLRVCND
Sbjct: 688 EIKKMGFAPDTESVLHDLEVEVKDQILRYHSEKLAIAFGIISTPENTTLRIMKNLRVCND 747

Query: 481 CHSAIKFISKLVGREIIVRDATRFHHFKDGSCSCRDYW 519
           CH+AIKFISKLV REIIVRDATRFHHFKDGSCSC+DYW
Sbjct: 748 CHNAIKFISKLVDREIIVRDATRFHHFKDGSCSCKDYW 785

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PP168_ARATH7.7e-21169.11Pentatricopeptide repeat-containing protein At2g22070 OS=Arabidopsis thaliana GN... [more]
PPR57_ARATH1.2e-12342.66Pentatricopeptide repeat-containing protein At1g25360 OS=Arabidopsis thaliana GN... [more]
PPR53_ARATH1.6e-12343.00Pentatricopeptide repeat-containing protein At1g20230 OS=Arabidopsis thaliana GN... [more]
PP301_ARATH1.9e-12143.71Pentatricopeptide repeat-containing protein At4g02750 OS=Arabidopsis thaliana GN... [more]
PP425_ARATH2.1e-12042.18Pentatricopeptide repeat-containing protein At5g48910 OS=Arabidopsis thaliana GN... [more]
Match NameE-valueIdentityDescription
B9HFU9_POPTR5.5e-23275.10Pentatricopeptide repeat-containing family protein OS=Populus trichocarpa GN=POP... [more]
B9HFU9_POPTR5.6e-2728.08Pentatricopeptide repeat-containing family protein OS=Populus trichocarpa GN=POP... [more]
F6H4P3_VITVI4.2e-3029.54Putative uncharacterized protein OS=Vitis vinifera GN=VIT_07s0031g01340 PE=4 SV=... [more]
A0A067KG88_JATCU1.3e-3130.26Uncharacterized protein OS=Jatropha curcas GN=JCGZ_09370 PE=4 SV=1[more]
A0A061DIY7_THECC1.6e-2631.47Pentatricopeptide (PPR) repeat-containing protein OS=Theobroma cacao GN=TCM_0012... [more]
Match NameE-valueIdentityDescription
AT2G22070.14.3e-21269.11 pentatricopeptide (PPR) repeat-containing protein[more]
AT1G25360.16.8e-12542.66 Pentatricopeptide repeat (PPR) superfamily protein[more]
AT1G20230.18.9e-12543.00 Pentatricopeptide repeat (PPR) superfamily protein[more]
AT4G02750.11.1e-12243.71 Tetratricopeptide repeat (TPR)-like superfamily protein[more]
AT5G48910.11.2e-12142.18 Pentatricopeptide repeat (PPR) superfamily protein[more]
Match NameE-valueIdentityDescription
gi|449449306|ref|XP_004142406.1|2.0e-299100.00PREDICTED: pentatricopeptide repeat-containing protein At2g22070 [Cucumis sativu... [more]
gi|449449306|ref|XP_004142406.1|5.4e-3130.27PREDICTED: pentatricopeptide repeat-containing protein At2g22070 [Cucumis sativu... [more]
gi|700197064|gb|KGN52241.1|8.7e-2125.08hypothetical protein Csa_5G622560 [Cucumis sativus][more]
gi|659091956|ref|XP_008446823.1|1.6e-3030.27PREDICTED: pentatricopeptide repeat-containing protein At2g22070 [Cucumis melo][more]
gi|743838060|ref|XP_011025626.1|3.6e-2728.46PREDICTED: pentatricopeptide repeat-containing protein At2g22070-like [Populus e... [more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR002885Pentatricopeptide_repeat
IPR011990TPR-like_helical_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0008270zinc ion binding
GO:0005515protein binding
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005515 protein binding
molecular_function GO:0008270 zinc ion binding

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Csa5G622560Csa5G622560gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Csa5G622560.2Csa5G622560.2-proteinpolypeptide


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csa5G622560.2.utr5p1Csa5G622560.2.utr5p1five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csa5G622560.2.cds1Csa5G622560.2.cds1CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csa5G622560.2.utr3p1Csa5G622560.2.utr3p1three_prime_UTR
Csa5G622560.2.utr3p2Csa5G622560.2.utr3p2three_prime_UTR
Csa5G622560.2.utr3p3Csa5G622560.2.utr3p3three_prime_UTR


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 352..380
score: 0.21coord: 184..204
score: 0.65coord: 285..309
score: 0
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 109..152
score: 7.5E-11coord: 211..257
score: 2.
IPR002885Pentatricopeptide repeatTIGRFAMsTIGR00756TIGR00756coord: 111..144
score: 7.2E-8coord: 81..111
score: 0.0025coord: 213..246
score: 2.
IPR002885Pentatricopeptide repeatPROFILEPS51375PPRcoord: 348..382
score: 8.681coord: 179..209
score: 5.821coord: 10..44
score: 5.623coord: 211..245
score: 11.334coord: 282..312
score: 7.004coord: 109..143
score: 12.419coord: 246..276
score: 6.807coord: 78..108
score: 8
IPR011990Tetratricopeptide-like helical domainGENE3DG3DSA:1.25.40.10coord: 211..241
score: 2.4E-7coord: 312..358
score: 2.4E-7coord: 94..139
score: 1.8E-7coord: 359..421
score: 1.
IPR011990Tetratricopeptide-like helical domainunknownSSF48452TPR-likecoord: 226..270
score: 2.28E-9coord: 89..133
score: 2.28E-9coord: 321..371
score: 2.2
NoneNo IPR availablePANTHERPTHR24015FAMILY NOT NAMEDcoord: 1..389
score:
NoneNo IPR availablePANTHERPTHR24015:SF43SUBFAMILY NOT NAMEDcoord: 1..389
score: