Csa5G517870.1 (mRNA) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa5G517870.1
Type	mRNA
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp; contains IPR000905 (Gcp-like domain), IPR017861 (Kae1/YgjD family)
Location	Chr5 : 18271852 .. 18272433 (+)
Sequence length	582

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAAAATGACAGCTTTGGGTTTTGAGGGTTCAGCAAACAAGATCGGTGTTGGGGTTGTAACTTTAGATGGCAACATTTTGTCAAACCCACGTCACACCTACATTACACCTCCTGGTCATGGCTTCCTTCCTCGTGAAACTGCACAACACCATCTCCACCACATTCTTCCATTGGTTAAGTCTGCTCTGGAAACTGCAAAAATTACCCCAAAGGATATTGACTGCCTTTGTTACACCAAAGGGCCTGGCATGGGAGCTCCTCTTCAGGTTTCTGCTGTTGCTGTGAGGGTTCTTTCTCAGATTTGGAATAAGCCTATTGTTGCTGTTAATCATTGTGTTGCACATATTGAGATGGGAAGGGTTGTAACTGGTGCAGATGATCCTGTTGTCTTGTATGTTAGTGGTGGGAATACTCAGGTGATTGCTTATAGTGAAGGAAGGTATAGAATCTTTGGGGAGACTATTGATATTGCTGTTGGAAATTGCTTGGACCGGTTTGCAAGAGTCCTAACACTATCCAATGACCCAAGTCCTGGATACAATATTGAACAGGTGTGTTACAGGAAAACGATAATATAG

mRNA sequence

ATGAAGAAAATGACAGCTTTGGGTTTTGAGGGTTCAGCAAACAAGATCGGTGTTGGGGTTGTAACTTTAGATGGCAACATTTTGTCAAACCCACGTCACACCTACATTACACCTCCTGGTCATGGCTTCCTTCCTCGTGAAACTGCACAACACCATCTCCACCACATTCTTCCATTGGTTAAGTCTGCTCTGGAAACTGCAAAAATTACCCCAAAGGATATTGACTGCCTTTGTTACACCAAAGGGCCTGGCATGGGAGCTCCTCTTCAGGTTTCTGCTGTTGCTGTGAGGGTTCTTTCTCAGATTTGGAATAAGCCTATTGTTGCTGTTAATCATTGTGTTGCACATATTGAGATGGGAAGGGTTGTAACTGGTGCAGATGATCCTGTTGTCTTGTATGTTAGTGGTGGGAATACTCAGGTGATTGCTTATAGTGAAGGAAGGTATAGAATCTTTGGGGAGACTATTGATATTGCTGTTGGAAATTGCTTGGACCGGTTTGCAAGAGTCCTAACACTATCCAATGACCCAAGTCCTGGATACAATATTGAACAGGTGTGTTACAGGAAAACGATAATATAG

Coding sequence (CDS)

ATGAAGAAAATGACAGCTTTGGGTTTTGAGGGTTCAGCAAACAAGATCGGTGTTGGGGTTGTAACTTTAGATGGCAACATTTTGTCAAACCCACGTCACACCTACATTACACCTCCTGGTCATGGCTTCCTTCCTCGTGAAACTGCACAACACCATCTCCACCACATTCTTCCATTGGTTAAGTCTGCTCTGGAAACTGCAAAAATTACCCCAAAGGATATTGACTGCCTTTGTTACACCAAAGGGCCTGGCATGGGAGCTCCTCTTCAGGTTTCTGCTGTTGCTGTGAGGGTTCTTTCTCAGATTTGGAATAAGCCTATTGTTGCTGTTAATCATTGTGTTGCACATATTGAGATGGGAAGGGTTGTAACTGGTGCAGATGATCCTGTTGTCTTGTATGTTAGTGGTGGGAATACTCAGGTGATTGCTTATAGTGAAGGAAGGTATAGAATCTTTGGGGAGACTATTGATATTGCTGTTGGAAATTGCTTGGACCGGTTTGCAAGAGTCCTAACACTATCCAATGACCCAAGTCCTGGATACAATATTGAACAGGTGTGTTACAGGAAAACGATAATATAG

Protein sequence

MKKMTALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAQHHLHHILPLVKSALETAKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQVCYRKTII*

Homology

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BLAST of Csa5G517870.1 vs. Swiss-Prot
Match: OSGEP_NEMVE (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Nematostella vectensis GN=osgep PE=3 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 9.3e-77
Identity = 129/182 (70.88%), Postives = 155/182 (85.16%), Query Frame = 1


Query: 5   TALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAQHHLHHILPLVKSAL 64
           T +GFEGSANK+G+G++  DG +LSNPRHTYITPPG GF+PR+TA+HH  H + +++ AL
Sbjct: 3   TVIGFEGSANKLGIGIIR-DGVVLSNPRHTYITPPGQGFMPRDTAKHHQEHAIDILRRAL 62

Query: 65  ETAKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVT 124
           + A+I P+DIDC+CYTKGPGMGAPL   AV  R ++Q+W KPI+ VNHC+ HIEMGR++T
Sbjct: 63  DEAQIRPQDIDCICYTKGPGMGAPLVAVAVVARTVAQLWKKPIIGVNHCIGHIEMGRLIT 122

Query: 125 GADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIE 184
           GA++P VLYVSGGNTQVIAY + RYRIFGETIDIAVGNCLDRFARVL LSNDPSPGYNIE
Sbjct: 123 GANNPTVLYVSGGNTQVIAYLQKRYRIFGETIDIAVGNCLDRFARVLKLSNDPSPGYNIE 182

Query: 185 QV 187
           Q+
Sbjct: 183 QM 183

[Close]

BLAST of Csa5G517870.1 vs. Swiss-Prot
Match: OSGEP_DICDI (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Dictyostelium discoideum GN=osgep PE=3 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 3.9e-75
Identity = 130/180 (72.22%), Postives = 154/180 (85.56%), Query Frame = 1


Query: 7   LGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAQHHLHHILPLVKSALET 66
           +GFEGSANK+G+G+V  DG ILSN RHT+ITPPG GFLP++TA+HH   IL LV+ +LE 
Sbjct: 5   MGFEGSANKLGIGIVKDDGTILSNIRHTFITPPGEGFLPKDTAKHHRSFILSLVEKSLEE 64

Query: 67  AKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGA 126
           +K+ P DIDCL YTKGPGMG PL+  AV VR+LSQ+W++PIVAVNHC+AHIEMGR++TGA
Sbjct: 65  SKLKPSDIDCLAYTKGPGMGPPLRSVAVTVRMLSQLWDRPIVAVNHCIAHIEMGRLITGA 124

Query: 127 DDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQV 186
            DP +LYVSGGNTQVI+YS  +YRIFGETIDIAVGNCLDRFARV+ + NDPSPGYNIEQ+
Sbjct: 125 VDPTILYVSGGNTQVISYSLKKYRIFGETIDIAVGNCLDRFARVIQIPNDPSPGYNIEQL 184

[Close]

BLAST of Csa5G517870.1 vs. Swiss-Prot
Match: OSGEP_BOVIN (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Bos taurus GN=OSGEP PE=2 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 2.1e-73
Identity = 132/183 (72.13%), Postives = 151/183 (82.51%), Query Frame = 1


Query: 7   LGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAQHHLHHILPLVKSALET 66
           LGFEGSANKIGVGVV  DG +L+NPR TY+TPPG GFLP +TA+HH   IL L++ AL  
Sbjct: 5   LGFEGSANKIGVGVVR-DGKVLANPRRTYVTPPGTGFLPGDTARHHRAVILDLLQEALTE 64

Query: 67  AKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGA 126
           A +T +DIDC+ YTKGPGMGAPL   AV  R ++Q+WNKP++ VNHC+ HIEMGR++TGA
Sbjct: 65  AGLTSEDIDCIAYTKGPGMGAPLVSVAVVARTVAQLWNKPLLGVNHCIGHIEMGRLITGA 124

Query: 127 DDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQV 186
            +P VLYVSGGNTQVIAYSE RYRIFGETIDIAVGNCLDRFARVL +SNDPSPGYNIEQ+
Sbjct: 125 TNPTVLYVSGGNTQVIAYSEHRYRIFGETIDIAVGNCLDRFARVLKISNDPSPGYNIEQM 184

Query: 187 CYR 190
             R
Sbjct: 185 AKR 186

[Close]

BLAST of Csa5G517870.1 vs. Swiss-Prot
Match: OSGEP_RAT (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Rattus norvegicus GN=Osgep PE=2 SV=2)

HSP 1 Score: 276.6 bits (706), Expect = 2.1e-73
Identity = 132/183 (72.13%), Postives = 151/183 (82.51%), Query Frame = 1


Query: 7   LGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAQHHLHHILPLVKSALET 66
           LGFEGSANKIGVGVV  DG +L+NPR TY+T PG GFLP +TA+HH   IL L++ AL  
Sbjct: 5   LGFEGSANKIGVGVVR-DGTVLANPRRTYVTAPGTGFLPGDTARHHRAVILDLLQEALTE 64

Query: 67  AKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGA 126
           A +TPKDIDC+ YTKGPGMGAPL   AV  R ++Q+WNKP++ VNHC+ HIEMGR++TGA
Sbjct: 65  AGLTPKDIDCIAYTKGPGMGAPLASVAVVARTVAQLWNKPLLGVNHCIGHIEMGRLITGA 124

Query: 127 DDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQV 186
            +P VLYVSGGNTQVI+YSE RYRIFGETIDIAVGNCLDRFARVL +SNDPSPGYNIEQ+
Sbjct: 125 VNPTVLYVSGGNTQVISYSEHRYRIFGETIDIAVGNCLDRFARVLKISNDPSPGYNIEQM 184

Query: 187 CYR 190
             R
Sbjct: 185 AKR 186

[Close]

BLAST of Csa5G517870.1 vs. Swiss-Prot
Match: OSGEP_HUMAN (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Homo sapiens GN=OSGEP PE=1 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 2.8e-73
Identity = 132/183 (72.13%), Postives = 150/183 (81.97%), Query Frame = 1


Query: 7   LGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAQHHLHHILPLVKSALET 66
           LGFEGSANKIGVGVV  DG +L+NPR TY+TPPG GFLP +TA+HH   IL L++ AL  
Sbjct: 5   LGFEGSANKIGVGVVR-DGKVLANPRRTYVTPPGTGFLPGDTARHHRAVILDLLQEALTE 64

Query: 67  AKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGA 126
           + +T +DIDC+ YTKGPGMGAPL   AV  R ++Q+WNKP+V VNHC+ HIEMGR++TGA
Sbjct: 65  SGLTSQDIDCIAYTKGPGMGAPLVSVAVVARTVAQLWNKPLVGVNHCIGHIEMGRLITGA 124

Query: 127 DDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQV 186
             P VLYVSGGNTQVIAYSE RYRIFGETIDIAVGNCLDRFARVL +SNDPSPGYNIEQ+
Sbjct: 125 TSPTVLYVSGGNTQVIAYSEHRYRIFGETIDIAVGNCLDRFARVLKISNDPSPGYNIEQM 184

Query: 187 CYR 190
             R
Sbjct: 185 AKR 186

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BLAST of Csa5G517870.1 vs. TrEMBL
Match: A0A0A0KR08_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G517870 PE=4 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 1.6e-107
Identity = 193/193 (100.00%), Postives = 193/193 (100.00%), Query Frame = 1


Query: 1   MKKMTALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAQHHLHHILPLV 60
           MKKMTALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAQHHLHHILPLV
Sbjct: 1   MKKMTALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAQHHLHHILPLV 60

Query: 61  KSALETAKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120
           KSALETAKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG
Sbjct: 61  KSALETAKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120

Query: 121 RVVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           RVVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG
Sbjct: 121 RVVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180

Query: 181 YNIEQVCYRKTII 194
           YNIEQVCYRKTII
Sbjct: 181 YNIEQVCYRKTII 193

[Close]

BLAST of Csa5G517870.1 vs. TrEMBL
Match: W9R4Z4_9ROSA (Putative tRNA threonylcarbamoyladenosine biosynthesis protein osgep OS=Morus notabilis GN=L484_024755 PE=4 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 2.1e-96
Identity = 173/186 (93.01%), Postives = 180/186 (96.77%), Query Frame = 1


Query: 1   MKKMTALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAQHHLHHILPLV 60
           MKKM ALGFEGSANKIGVGVVTLDG ILSNPRHTYITPPG GFLPRETAQHHL H+LPLV
Sbjct: 1   MKKMIALGFEGSANKIGVGVVTLDGTILSNPRHTYITPPGQGFLPRETAQHHLQHVLPLV 60

Query: 61  KSALETAKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120
           KSALETA+ITP++IDCLCYTKGPGMGAPLQVSAV VRVLSQ+W KPIVAVNHCVAHIEMG
Sbjct: 61  KSALETAQITPEEIDCLCYTKGPGMGAPLQVSAVVVRVLSQLWKKPIVAVNHCVAHIEMG 120

Query: 121 RVVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           R+VTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG
Sbjct: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180

Query: 181 YNIEQV 187
           YNIEQ+
Sbjct: 181 YNIEQL 186

[Close]

BLAST of Csa5G517870.1 vs. TrEMBL
Match: M5XC29_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007957mg PE=4 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 2.1e-96
Identity = 172/186 (92.47%), Postives = 180/186 (96.77%), Query Frame = 1


Query: 1   MKKMTALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAQHHLHHILPLV 60
           MKKM ALGFEGSANKIGVGVVTLDG ILSNPRHTYITPPG GFLPRETAQHHL HILPL+
Sbjct: 1   MKKMIALGFEGSANKIGVGVVTLDGTILSNPRHTYITPPGQGFLPRETAQHHLQHILPLI 60

Query: 61  KSALETAKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120
           KSALETA+ITPK+IDCLCYTKGPGMGAPLQV+A+ VRVLSQ+W KPIVAVNHCVAHIEMG
Sbjct: 61  KSALETAQITPKEIDCLCYTKGPGMGAPLQVAAIVVRVLSQLWKKPIVAVNHCVAHIEMG 120

Query: 121 RVVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           R+VTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG
Sbjct: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180

Query: 181 YNIEQV 187
           YNIEQ+
Sbjct: 181 YNIEQL 186

[Close]

BLAST of Csa5G517870.1 vs. TrEMBL
Match: A0A0L9VKY4_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan10g160700 PE=4 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 1.8e-95
Identity = 169/186 (90.86%), Postives = 178/186 (95.70%), Query Frame = 1


Query: 1   MKKMTALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAQHHLHHILPLV 60
           MK+M ALGFEGSANKIGVGVVTLDG ILSNPRHTYITPPG GFLPRETAQHHL H+LPLV
Sbjct: 1   MKRMIALGFEGSANKIGVGVVTLDGAILSNPRHTYITPPGQGFLPRETAQHHLQHVLPLV 60

Query: 61  KSALETAKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120
           KSALETAK+TP+DIDC+CYTKGPGMGAPLQVSA+ VRVLSQ+W KPIV VNHCVAH+EMG
Sbjct: 61  KSALETAKVTPEDIDCICYTKGPGMGAPLQVSAIVVRVLSQLWKKPIVGVNHCVAHVEMG 120

Query: 121 RVVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           R+VTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVL LSNDPSPG
Sbjct: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLNLSNDPSPG 180

Query: 181 YNIEQV 187
           YNIEQV
Sbjct: 181 YNIEQV 186

[Close]

BLAST of Csa5G517870.1 vs. TrEMBL
Match: A0A0S3S516_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.05G130600 PE=4 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 1.8e-95
Identity = 169/186 (90.86%), Postives = 178/186 (95.70%), Query Frame = 1


Query: 1   MKKMTALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAQHHLHHILPLV 60
           MK+M ALGFEGSANKIGVGVVTLDG ILSNPRHTYITPPG GFLPRETAQHHL H+LPLV
Sbjct: 1   MKRMIALGFEGSANKIGVGVVTLDGAILSNPRHTYITPPGQGFLPRETAQHHLQHVLPLV 60

Query: 61  KSALETAKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120
           KSALETAK+TP+DIDC+CYTKGPGMGAPLQVSA+ VRVLSQ+W KPIV VNHCVAH+EMG
Sbjct: 61  KSALETAKVTPEDIDCICYTKGPGMGAPLQVSAIVVRVLSQLWKKPIVGVNHCVAHVEMG 120

Query: 121 RVVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           R+VTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVL LSNDPSPG
Sbjct: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLNLSNDPSPG 180

Query: 181 YNIEQV 187
           YNIEQV
Sbjct: 181 YNIEQV 186

[Close]

BLAST of Csa5G517870.1 vs. TAIR10
Match: AT4G22720.1 (AT4G22720.1 Actin-like ATPase superfamily protein)

HSP 1 Score: 351.7 bits (901), Expect = 2.9e-97
Identity = 165/185 (89.19%), Postives = 176/185 (95.14%), Query Frame = 1


Query: 2   KKMTALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAQHHLHHILPLVK 61
           KKM A+GFEGSANKIGVG+VTLDG IL+NPRHTYITPPGHGFLPRETA HHL H+LPLVK
Sbjct: 3   KKMIAIGFEGSANKIGVGIVTLDGTILANPRHTYITPPGHGFLPRETAHHHLDHVLPLVK 62

Query: 62  SALETAKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGR 121
           SALET+++TP++IDC+CYTKGPGMGAPLQVSA+ VRVLSQ+W KPIVAVNHCVAHIEMGR
Sbjct: 63  SALETSQVTPEEIDCICYTKGPGMGAPLQVSAIVVRVLSQLWKKPIVAVNHCVAHIEMGR 122

Query: 122 VVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGY 181
           VVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVL LSNDPSPGY
Sbjct: 123 VVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLKLSNDPSPGY 182

Query: 182 NIEQV 187
           NIEQ+
Sbjct: 183 NIEQL 187

[Close]

BLAST of Csa5G517870.1 vs. TAIR10
Match: AT2G45270.1 (AT2G45270.1 glycoprotease 1)

HSP 1 Score: 81.6 bits (200), Expect = 5.7e-16
Identity = 58/188 (30.85%), Postives = 91/188 (48.40%), Query Frame = 1


Query: 4   MTALGFEGSANKIGVGVVTLDGNILSN--PRHTYITPPGHGFLPRETAQHHLHHILPLVK 63
           +  LG E S +     VV  +G ILS        +     G  P++  + H   I  +V+
Sbjct: 84  LVVLGIETSCDDTAAAVVRGNGEILSQVISSQAELLVQYGGVAPKQAEEAHSRVIDKVVQ 143

Query: 64  SALETAKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGR 123
            AL+ A +T KD+  +  T GPG+   L+V     R ++  ++ PIV V+H  AH  + R
Sbjct: 144 DALDKANLTEKDLSAVAVTIGPGLSLCLRVGVRKARRVAGNFSLPIVGVHHMEAHALVAR 203

Query: 124 VVTG--ADDPVVLYVSGG-NTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPS 183
           +V    +   + L +SGG N  V+A+  G+Y   G T+D A+G   D+ A+ L L    S
Sbjct: 204 LVEQELSFPFMALLISGGHNLLVLAHKLGQYTQLGTTVDDAIGEAFDKTAKWLGLDMHRS 263

Query: 184 PGYNIEQV 187
            G  +E++
Sbjct: 264 GGPAVEEL 271

[Close]

BLAST of Csa5G517870.1 vs. NCBI nr
Match: gi|700196162|gb|KGN51339.1| (hypothetical protein Csa_5G517870 [Cucumis sativus])

HSP 1 Score: 396.7 bits (1018), Expect = 2.3e-107
Identity = 193/193 (100.00%), Postives = 193/193 (100.00%), Query Frame = 1


Query: 1   MKKMTALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAQHHLHHILPLV 60
           MKKMTALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAQHHLHHILPLV
Sbjct: 1   MKKMTALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAQHHLHHILPLV 60

Query: 61  KSALETAKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120
           KSALETAKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG
Sbjct: 61  KSALETAKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120

Query: 121 RVVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           RVVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG
Sbjct: 121 RVVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180

Query: 181 YNIEQVCYRKTII 194
           YNIEQVCYRKTII
Sbjct: 181 YNIEQVCYRKTII 193

[Close]

BLAST of Csa5G517870.1 vs. NCBI nr
Match: gi|449450050|ref|XP_004142777.1| (PREDICTED: probable tRNA N6-adenosine threonylcarbamoyltransferase [Cucumis sativus])

HSP 1 Score: 380.6 bits (976), Expect = 1.7e-102
Identity = 185/186 (99.46%), Postives = 186/186 (100.00%), Query Frame = 1


Query: 1   MKKMTALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAQHHLHHILPLV 60
           MKKMTALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAQHHLHHILPLV
Sbjct: 1   MKKMTALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAQHHLHHILPLV 60

Query: 61  KSALETAKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120
           KSALETAKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG
Sbjct: 61  KSALETAKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120

Query: 121 RVVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           RVVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG
Sbjct: 121 RVVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180

Query: 181 YNIEQV 187
           YNIEQ+
Sbjct: 181 YNIEQL 186

[Close]

BLAST of Csa5G517870.1 vs. NCBI nr
Match: gi|645231617|ref|XP_008222479.1| (PREDICTED: probable tRNA N6-adenosine threonylcarbamoyltransferase [Prunus mume])

HSP 1 Score: 359.8 bits (922), Expect = 3.1e-96
Identity = 172/186 (92.47%), Postives = 180/186 (96.77%), Query Frame = 1


Query: 1   MKKMTALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAQHHLHHILPLV 60
           MKKM ALGFEGSANKIGVGVVTLDG ILSNPRHTYITPPG GFLPRETAQHHL HILPL+
Sbjct: 1   MKKMIALGFEGSANKIGVGVVTLDGTILSNPRHTYITPPGQGFLPRETAQHHLQHILPLI 60

Query: 61  KSALETAKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120
           KSALETA+ITPK+IDCLCYTKGPGMGAPLQV+A+ VRVLSQ+W KPIVAVNHCVAHIEMG
Sbjct: 61  KSALETAQITPKEIDCLCYTKGPGMGAPLQVAAIVVRVLSQLWKKPIVAVNHCVAHIEMG 120

Query: 121 RVVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           R+VTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG
Sbjct: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180

Query: 181 YNIEQV 187
           YNIEQ+
Sbjct: 181 YNIEQL 186

[Close]

BLAST of Csa5G517870.1 vs. NCBI nr
Match: gi|703102734|ref|XP_010097542.1| (putative tRNA threonylcarbamoyladenosine biosynthesis protein osgep [Morus notabilis])

HSP 1 Score: 359.8 bits (922), Expect = 3.1e-96
Identity = 173/186 (93.01%), Postives = 180/186 (96.77%), Query Frame = 1


Query: 1   MKKMTALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAQHHLHHILPLV 60
           MKKM ALGFEGSANKIGVGVVTLDG ILSNPRHTYITPPG GFLPRETAQHHL H+LPLV
Sbjct: 1   MKKMIALGFEGSANKIGVGVVTLDGTILSNPRHTYITPPGQGFLPRETAQHHLQHVLPLV 60

Query: 61  KSALETAKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120
           KSALETA+ITP++IDCLCYTKGPGMGAPLQVSAV VRVLSQ+W KPIVAVNHCVAHIEMG
Sbjct: 61  KSALETAQITPEEIDCLCYTKGPGMGAPLQVSAVVVRVLSQLWKKPIVAVNHCVAHIEMG 120

Query: 121 RVVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           R+VTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG
Sbjct: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180

Query: 181 YNIEQV 187
           YNIEQ+
Sbjct: 181 YNIEQL 186

[Close]

BLAST of Csa5G517870.1 vs. NCBI nr
Match: gi|596134493|ref|XP_007222270.1| (hypothetical protein PRUPE_ppa007957mg [Prunus persica])

HSP 1 Score: 359.8 bits (922), Expect = 3.1e-96
Identity = 172/186 (92.47%), Postives = 180/186 (96.77%), Query Frame = 1


Query: 1   MKKMTALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAQHHLHHILPLV 60
           MKKM ALGFEGSANKIGVGVVTLDG ILSNPRHTYITPPG GFLPRETAQHHL HILPL+
Sbjct: 1   MKKMIALGFEGSANKIGVGVVTLDGTILSNPRHTYITPPGQGFLPRETAQHHLQHILPLI 60

Query: 61  KSALETAKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120
           KSALETA+ITPK+IDCLCYTKGPGMGAPLQV+A+ VRVLSQ+W KPIVAVNHCVAHIEMG
Sbjct: 61  KSALETAQITPKEIDCLCYTKGPGMGAPLQVAAIVVRVLSQLWKKPIVAVNHCVAHIEMG 120

Query: 121 RVVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           R+VTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG
Sbjct: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180

Query: 181 YNIEQV 187
           YNIEQ+
Sbjct: 181 YNIEQL 186

The following BLAST results are available for this feature:

BLAST of Csa5G517870.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
OSGEP_NEMVE	9.3e-77	70.88	Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Nematostella vectensi...	[more]
OSGEP_DICDI	3.9e-75	72.22	Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Dictyostelium discoid...	[more]
OSGEP_BOVIN	2.1e-73	72.13	Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Bos taurus GN=OSGEP P...	[more]
OSGEP_RAT	2.1e-73	72.13	Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Rattus norvegicus GN=...	[more]
OSGEP_HUMAN	2.8e-73	72.13	Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Homo sapiens GN=OSGEP...	[more]

BLAST of Csa5G517870.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KR08_CUCSA	1.6e-107	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G517870 PE=4 SV=1	[more]
W9R4Z4_9ROSA	2.1e-96	93.01	Putative tRNA threonylcarbamoyladenosine biosynthesis protein osgep OS=Morus not...	[more]
M5XC29_PRUPE	2.1e-96	92.47	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007957mg PE=4 SV=1	[more]
A0A0L9VKY4_PHAAN	1.8e-95	90.86	Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan10g160700 PE=4 SV=1	[more]
A0A0S3S516_PHAAN	1.8e-95	90.86	Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.05G130600 PE=...	[more]

BLAST of Csa5G517870.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G22720.1	2.9e-97	89.19	Actin-like ATPase superfamily protein	[more]
AT2G45270.1	5.7e-16	30.85	glycoprotease 1	[more]

BLAST of Csa5G517870.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700196162\|gb\|KGN51339.1\|	2.3e-107	100.00	hypothetical protein Csa_5G517870 [Cucumis sativus]	[more]
gi\|449450050\|ref\|XP_004142777.1\|	1.7e-102	99.46	PREDICTED: probable tRNA N6-adenosine threonylcarbamoyltransferase [Cucumis sati...	[more]
gi\|645231617\|ref\|XP_008222479.1\|	3.1e-96	92.47	PREDICTED: probable tRNA N6-adenosine threonylcarbamoyltransferase [Prunus mume]	[more]
gi\|703102734\|ref\|XP_010097542.1\|	3.1e-96	93.01	putative tRNA threonylcarbamoyladenosine biosynthesis protein osgep [Morus notab...	[more]
gi\|596134493\|ref\|XP_007222270.1\|	3.1e-96	92.47	hypothetical protein PRUPE_ppa007957mg [Prunus persica]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR000905	Gcp-like_dom
IPR017861	KAE1/TsaD

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
cellular_component	GO:0005829	cytosol
molecular_function	GO:0016787	hydrolase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Csa5G517870	Csa5G517870	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Csa5G517870.1	Csa5G517870.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csa5G517870.1.cds1	Csa5G517870.1.cds1	CDS

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000905	Gcp-like domain	PFAM	PF00814	Peptidase_M22	coord: 28..186 score: 2.3
IPR017861	Kae1/TsaD family	PRINTS	PR00789	OSIALOPTASE	coord: 152..173 score: 5.1E-34coord: 7..20 score: 5.1E-34coord: 98..117 score: 5.1E-34coord: 130..142 score: 5.1E-34coord: 77..97 score: 5.1
None	No IPR available	GENE3D	G3DSA:3.30.420.40		coord: 4..157 score: 3.4
None	No IPR available	PANTHER	PTHR11735	O-SIALOGLYCOPROTEIN ENDOPEPTIDASE	coord: 1..186 score: 1.6E
None	No IPR available	PANTHER	PTHR11735:SF14	TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE-RELATED	coord: 1..186 score: 1.6E
None	No IPR available	unknown	SSF53067	Actin-like ATPase domain	coord: 7..124 score: 9.32E-25coord: 103..171 score: 2.3