Csa3G178520.1 (mRNA) Cucumber (Chinese Long) v2

BLAST of Csa3G178520.1 vs. Swiss-Prot
Match: SBT13_ARATH (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 1157.5 bits (2993), Expect = 0.0e+00
Identity = 551/760 (72.50%), Postives = 651/760 (85.66%), Query Frame = 1

Query: 46  LLFLDSFLLSSALFLKTYVVQMDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEE 105
           L+FL +   +     KTYV+ MD+SAMP  +TNH +WYS+ + +V     +E  G     
Sbjct: 20  LIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNN-- 79

Query: 106 RIIYGYHNVFHGVAARLSEEEVEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNS 165
           RI+Y Y   FHG+AA+L++EE E+LEEEDGVVA+ PE +YELHTTRSP FLGLE  +S  
Sbjct: 80  RILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESER 139

Query: 166 AWSQQIADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVG 225
            W++++ DHDVVVGVLDTGIWPES+SF+D GMSPVPA W+G CETG+ F K+NCNRKIVG
Sbjct: 140 VWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVG 199

Query: 226 ARVFYRGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMA 285
           ARVFYRGY+AATGK +E+LEYKSPRD+DGHGTHTAATVAGSPV GA+L G+AYGTARGMA
Sbjct: 200 ARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMA 259

Query: 286 PGARIAAYKVCWIGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAM 345
             AR+AAYKVCW+GGCFSSDILSAVD+AVADGV VLSISLGGGVS+Y RDSLS+A FGAM
Sbjct: 260 QKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAM 319

Query: 346 EMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGR 405
           EMGVFVSCSAGNGGPDP+SLTNVSPWITTVGASTMDRDFPA VK+G  RT  GVSLY+GR
Sbjct: 320 EMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGR 379

Query: 406 ITIPENKQFPIVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKN 465
             +P+NKQ+P+VY+G N+SSPDP+S CL+G LD   VAGKIVICDRG++PRVQKG VVK 
Sbjct: 380 TVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKR 439

Query: 466 AGGIGMILSNTAANGEELVADCHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLG 525
           AGGIGM+L+NTA NGEELVAD H++PAVA+GE+EGK IKQYA+T+++ATA+L  LGTR+G
Sbjct: 440 AGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIG 499

Query: 526 VKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILS 585
           +KPSPVVAAFSSRGPNFL+LEILKPDL+APGVNILAAWTG   PSSL++D RRVKFNILS
Sbjct: 500 IKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILS 559

Query: 586 GTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDH 645
           GTSMSCPHVSGVAALIKS+HPDWSP+AIKSALMTTAYVHDN +KPL D+S A+PSSPYDH
Sbjct: 560 GTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDH 619

Query: 646 GAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLSPTQLKVFSKYSNRTCR-GLLPNPGDL 705
           GAGHI+P +A DPGLVY+I PQ+YF+FLCTQDLSP+QLKVF+K+SNRTC+  L  NPG+L
Sbjct: 620 GAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNL 679

Query: 706 NYPAISAVFPEKTTVTSLTLHRTVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYE 765
           NYPAISA+FPE T V ++TL RTVTNVGP  SSY   VSPFKGA+V V+P++LNFT +++
Sbjct: 680 NYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQ 739

Query: 766 KVSYRITFVTKKRQSMPEFGGLIWKDGSHKVRSPIVITWL 805
           K+SY +TF T+ R   PEFGGL+WK  +HKVRSP++ITWL
Sbjct: 740 KLSYTVTFRTRFRMKRPEFGGLVWKSTTHKVRSPVIITWL 777

BLAST of Csa3G178520.1 vs. Swiss-Prot
Match: SBT17_ARATH (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 805.4 bits (2079), Expect = 5.5e-232
Identity = 411/772 (53.24%), Postives = 523/772 (67.75%), Query Frame = 1

Query: 51  SFLLSSALFLK----------------TYVVQMDRSAMPDSFTNHFEWYSNVLTNVVLDL 110
           SFL S+A FL                 TY+V M +S MP SF  H  WY + L ++    
Sbjct: 4   SFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSI---- 63

Query: 111 QREGNGGGGEERIIYGYHNVFHGVAARLSEEEVEKLEEEDGVVAIFPEMKYELHTTRSPR 170
                       ++Y Y N  HG + RL++EE + L  + GV+++ PE +YELHTTR+P 
Sbjct: 64  -------SDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPL 123

Query: 171 FLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWKGECETGRGF 230
           FLGL+   ++  + +  +  DVVVGVLDTG+WPES S+ D G  P+P+ WKG CE G  F
Sbjct: 124 FLGLDEHTAD-LFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNF 183

Query: 231 TKQNCNRKIVGARVFYRGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL 290
           T   CNRK++GAR F RGY++  G  +E  E +SPRD DGHGTHT++T AGS V GASLL
Sbjct: 184 TASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLL 243

Query: 291 GYAYGTARGMAPGARIAAYKVCWIGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR 350
           GYA GTARGMAP AR+A YKVCW+GGCFSSDIL+A+D+A+AD VNVLS+SLGGG+S YYR
Sbjct: 244 GYASGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYR 303

Query: 351 DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR 410
           D +++ AF AME G+ VSCSAGN GP   SL+NV+PWITTVGA T+DRDFPA+  LG+G+
Sbjct: 304 DGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGK 363

Query: 411 TITGVSLYRGRITIPENKQFPIVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGIS 470
             TGVSL++G   +P+ K  P +Y G N+S+    +LC+ GTL P  V GKIV+CDRGI+
Sbjct: 364 NFTGVSLFKGE-ALPD-KLLPFIYAG-NASNATNGNLCMTGTLIPEKVKGKIVMCDRGIN 423

Query: 471 PRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAIGEREGKAIKQYALTNRRAT 530
            RVQKG VVK AGG+GMIL+NTAANGEELVAD HL+PA  +GE+ G  I+ Y  T+   T
Sbjct: 424 ARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPT 483

Query: 531 ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT 590
           A++  LGT +GVKPSPVVAAFSSRGPN +T  ILKPDL+APGVNILAAWTG  GP+ L +
Sbjct: 484 ASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLAS 543

Query: 591 DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDS 650
           D+RRV+FNI+SGTSMSCPHVSG+AAL+KS HP+WSP+AI+SALMTTAY      KPL D 
Sbjct: 544 DSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDI 603

Query: 651 SAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLSPTQLKVFSKYSNRTC 710
           +   PS+P+DHGAGH++P  A +PGL+Y++  +DY  FLC  + +  Q++  S+  N TC
Sbjct: 604 ATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSR-RNYTC 663

Query: 711 -RGLLPNPGDLNYPAISAVFPEKTTVTSLTLHRTVTNVGPATSSYHAVVSPFKGATVKVE 770
                 +  DLNYP+ +        V +    RTVT+VG A +    V S   G  + VE
Sbjct: 664 DPSKSYSVADLNYPSFAV---NVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVE 723

Query: 771 PESLNFTRRYEKVSYRITFV--TKKRQSMPEFGGLIWKDGSHKVRSPIVITW 804
           P  LNF    EK SY +TF   + K      FG + W DG H V SP+ I+W
Sbjct: 724 PAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Csa3G178520.1 vs. Swiss-Prot
Match: SBT18_ARATH (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana GN=SBT1.8 PE=2 SV=1)

HSP 1 Score: 768.1 bits (1982), Expect = 9.7e-221
Identity = 390/760 (51.32%), Postives = 509/760 (66.97%), Query Frame = 1

Query: 52  FLLSSALFLKTYVVQMDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGY 111
           FLL      KTY+++++ S  P+SF  H +WY++ L +              E  ++Y Y
Sbjct: 19  FLLLHTTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNS--------------ESSLLYTY 78

Query: 112 HNVFHGVAARLSEEEVEKL-EEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQ 171
              FHG +A L   E + L    + ++ IF +  Y LHTTR+P FLGL            
Sbjct: 79  TTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLN--SEFGVHDLG 138

Query: 172 IADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFY 231
            + + V++GVLDTG+WPES SFDD  M  +P+ WKGECE+G  F  + CN+K++GAR F 
Sbjct: 139 SSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFS 198

Query: 232 RGYQAATGK-FNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGAR 291
           +G+Q A+G  F+ + E  SPRD DGHGTHT+ T AGS V  AS LGYA GTARGMA  AR
Sbjct: 199 KGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRAR 258

Query: 292 IAAYKVCWIGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGV 351
           +A YKVCW  GCF SDIL+A+DRA+ DGV+VLS+SLGGG + YYRD++++ AF AME GV
Sbjct: 259 VATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGV 318

Query: 352 FVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIP 411
           FVSCSAGN GP   S+ NV+PW+ TVGA T+DRDFPA   LG+G+ +TGVSLY G     
Sbjct: 319 FVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSG--VGM 378

Query: 412 ENKQFPIVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGI 471
             K   +VY   NSSS   S+LCL G+LD   V GKIV+CDRG++ RV+KG VV++AGG+
Sbjct: 379 GTKPLELVYNKGNSSS---SNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGL 438

Query: 472 GMILSNTAANGEELVADCHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPS 531
           GMI++NTAA+GEELVAD HL+PA+A+G++ G  +++Y  ++ + TA L F GT L VKPS
Sbjct: 439 GMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPS 498

Query: 532 PVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSM 591
           PVVAAFSSRGPN +T EILKPD++ PGVNILA W+   GP+ L  D+RR +FNI+SGTSM
Sbjct: 499 PVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSM 558

Query: 592 SCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGH 651
           SCPH+SG+A L+K+ HP+WSPSAIKSALMTTAYV DNT  PL D++  S S+PY HG+GH
Sbjct: 559 SCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGH 618

Query: 652 INPRKALDPGLVYEIQPQDYFDFLCTQDLSPTQLKVFSKYSNRTCRGLLPNPGDLNYPAI 711
           ++P+KAL PGLVY+I  ++Y  FLC+ D +   +    K  +  C     +PG LNYP+ 
Sbjct: 619 VDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSF 678

Query: 712 SAVFPEKTTVTSLTLHRTVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYR 771
           S +F  K  V      R VTNVG A+S Y   V+      + V+P  L+F    EK  Y 
Sbjct: 679 SVLFGGKRVV---RYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYT 738

Query: 772 ITFVTKKRQSM---PEFGGLIWKDGSHKVRSPIVITWLSF 807
           +TFV+KK  SM    EFG + W +  H+VRSP+  +W  F
Sbjct: 739 VTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSWNRF 754

BLAST of Csa3G178520.1 vs. Swiss-Prot
Match: SBT16_ARATH (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 756.5 bits (1952), Expect = 2.9e-217
Identity = 395/782 (50.51%), Postives = 519/782 (66.37%), Query Frame = 1

Query: 35  MADSPLKCLCFLLF-LDSFLLSSALFLKTYVVQMDRSAMPDSFTNHFEWYSNVLTNVVLD 94
           MA S +  L FL F   SF  S A   KT++ ++D  +MP  F  H+ WYS         
Sbjct: 1   MASSTIVLLLFLSFPFISFAASQAA--KTFIFRIDGGSMPSIFPTHYHWYSTEFAE---- 60

Query: 95  LQREGNGGGGEERIIYGYHNVFHGVAARLSEEEVEKLEEEDGVVAIFPEMKYELHTTRSP 154
                     E RI++ YH VFHG +A ++ +E + L     V+A+F + + ELHTTRSP
Sbjct: 61  ----------ESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSP 120

Query: 155 RFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWKGECETGRG 214
           +FLGL+  +    WS+     DV++GV DTGIWPE  SF D  + P+P  W+G CE+G  
Sbjct: 121 QFLGLQ--NQKGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGAR 180

Query: 215 FTKQNCNRKIVGARVFYRGYQAAT-GKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGAS 274
           F+ +NCNRKI+GAR F +G QAA  G  N+ +E+ SPRD DGHGTHT++T AG     AS
Sbjct: 181 FSPRNCNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKAS 240

Query: 275 LLGYAYGTARGMAPGARIAAYKVCWI-GGCFSSDILSAVDRAVADGVNVLSISLGGG--- 334
           + GYA G A+G+AP ARIAAYKVCW   GC  SDIL+A D AV DGV+V+SIS+GGG   
Sbjct: 241 MSGYASGVAKGVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGI 300

Query: 335 VSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIV 394
            S YY D +++ ++GA   G+FVS SAGN GP+ +S+TN++PW+TTVGAST+DR+FPA  
Sbjct: 301 TSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADA 360

Query: 395 KLGDGRTITGVSLYRGRITIPEN-KQFPIVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIV 454
            LGDG  + GVSLY G   +P N + FP+VY G +  S   +SLC+E TLDP  V GKIV
Sbjct: 361 ILGDGHRLRGVSLYAG---VPLNGRMFPVVYPGKSGMSS--ASLCMENTLDPKQVRGKIV 420

Query: 455 ICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAIGEREGKAIKQYA 514
           ICDRG SPRV KG+VVK AGG+GMIL+N A+NGE LV D HL+PA A+G  EG  IK YA
Sbjct: 421 ICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYA 480

Query: 515 LTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKT 574
            ++    A++ F GT +G+KP+PV+A+FS RGPN L+ EILKPDL+APGVNILAAWT   
Sbjct: 481 SSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAV 540

Query: 575 GPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNT 634
           GP+ L +D R+ +FNILSGTSM+CPHVSG AAL+KS HPDWSP+ I+SA+MTT  + DN+
Sbjct: 541 GPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNS 600

Query: 635 YKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLSPTQLKVFS 694
            + L D S    ++PYD+G+GH+N  +A++PGLVY+I   DY  FLC+    P  ++V +
Sbjct: 601 NRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVIT 660

Query: 695 KYSNRTCRGLLPNPGDLNYPAISAVFP-EKTTVTSLTLHRTVTNVGPATSSYHAVVSPFK 754
           +   R      P+PG+LNYP+I+AVFP  +  + S T+ RT TNVG A + Y A +   +
Sbjct: 661 RTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPR 720

Query: 755 GATVKVEPESLNFTRRYEKVSYRITFVTKKR-----QSMPEFGGLIWKD-GSHKVRSPIV 803
           G TV V+P  L FT   ++ SY +T     R     ++   FG + W D G H VRSPIV
Sbjct: 721 GVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIV 759

BLAST of Csa3G178520.1 vs. Swiss-Prot
Match: SBT15_ARATH (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 754.6 bits (1947), Expect = 1.1e-216
Identity = 379/757 (50.07%), Postives = 513/757 (67.77%), Query Frame = 1

Query: 62  TYVVQMDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAAR 121
           TY+V +D  A P  F  HF WY++ L ++                II+ Y  VFHG +AR
Sbjct: 27  TYIVHVDHEAKPSIFPTHFHWYTSSLASLT----------SSPPSIIHTYDTVFHGFSAR 86

Query: 122 LSEEEVEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVL 181
           L+ ++  +L +   V+++ PE    LHTTRSP FLGL   D      +     D+V+GV+
Sbjct: 87  LTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVI 146

Query: 182 DTGIWPESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGKFN 241
           DTG+WPE  SFDD G+ PVP  WKG+C   + F +  CNRK+VGAR F  GY+A  GK N
Sbjct: 147 DTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMN 206

Query: 242 EQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGC 301
           E  E++SPRD DGHGTHTA+  AG  V  AS LGYA+G A GMAP AR+AAYKVCW  GC
Sbjct: 207 ETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGC 266

Query: 302 FSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPD 361
           + SDIL+A D AVADGV+V+S+S+GG V  YY D++++ AFGA++ G+FVS SAGNGGP 
Sbjct: 267 YDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPG 326

Query: 362 PVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGS 421
            +++TNV+PW+TTVGA T+DRDFPA VKLG+G+ I+GVS+Y G    P  + +P+VY GS
Sbjct: 327 ALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDP-GRMYPLVYGGS 386

Query: 422 NSSSPD-PSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANG 481
                   SSLCLEG+LDP+ V GKIV+CDRGI+ R  KG +V+  GG+GMI++N   +G
Sbjct: 387 LLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDG 446

Query: 482 EELVADCHLVPAVAIGEREGKAIKQY------ALTNRRATATLGFLGTRLGVKPSPVVAA 541
           E LVADCH++PA ++G   G  I++Y      + +++  TAT+ F GTRLG++P+PVVA+
Sbjct: 447 EGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVAS 506

Query: 542 FSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHV 601
           FS+RGPN  T EILKPD++APG+NILAAW  + GPS +T+D RR +FNILSGTSM+CPHV
Sbjct: 507 FSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHV 566

Query: 602 SGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRK 661
           SG+AAL+K+ HPDWSP+AI+SAL+TTAY  DN+ +P+ D S  + SS  D+G+GH++P K
Sbjct: 567 SGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTK 626

Query: 662 ALDPGLVYEIQPQDYFDFLCTQDLSPTQLKVFSKYSNRTCRGL--LPNPGDLNYPAISAV 721
           A+DPGLVY+I   DY +FLC  + + T +   ++     C G     + G+LNYP+ S V
Sbjct: 627 AMDPGLVYDITSYDYINFLCNSNYTRTNIVTITR-RQADCDGARRAGHVGNLNYPSFSVV 686

Query: 722 FPEKTTVTSLTLH--RTVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRI 781
           F ++   + ++ H  RTVTNVG + S Y   + P +G TV VEPE L+F R  +K+S+ +
Sbjct: 687 F-QQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVV 746

Query: 782 TFVTKKRQSMP-----EFGGLIWKDGSHKVRSPIVIT 803
              T + +  P     E G ++W DG   V SP+V+T
Sbjct: 747 RVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of Csa3G178520.1 vs. TrEMBL
Match: A0A061G0C3_THECC (Subtilase 1.3 OS=Theobroma cacao GN=TCM_015025 PE=4 SV=1)

HSP 1 Score: 1243.8 bits (3217), Expect = 0.0e+00
Identity = 607/780 (77.82%), Postives = 688/780 (88.21%), Query Frame = 1

Query: 35  MADSPLK--------CLCFLLFLDSFLLSSALFLKTYVVQMDRSAMPDSFTNHFEWYSNV 94
           M ++P+K        CL F L L +   S+ L  KT++VQMD+SAMP SF++H EWYS+ 
Sbjct: 1   MTENPVKWLFLILTSCLSFALVLSA---SNPLIKKTFIVQMDKSAMPASFSSHLEWYSSK 60

Query: 95  LTNVVL-DLQREGNGGGGEERIIYGYHNVFHGVAARLSEEEVEKLEEEDGVVAIFPEMKY 154
           + +V++ + Q EG+G G  ERIIY Y N FHGVAA+L+E+E E+LEEEDGVVAI PEMKY
Sbjct: 61  VKSVIMSNTQSEGDGDG--ERIIYSYQNAFHGVAAQLTEDEAERLEEEDGVVAILPEMKY 120

Query: 155 ELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWK 214
           +LHTTRSP FLGLEP +S S WSQ++ DHDV+VGVLDTGIWPES+SF+D G++PVPAHWK
Sbjct: 121 QLHTTRSPMFLGLEPEESTSIWSQKLTDHDVIVGVLDTGIWPESESFNDTGLAPVPAHWK 180

Query: 215 GECETGRGFTKQNCNRKIVGARVFYRGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAG 274
           G CETGRGF K +CNRKIVGARVFYRGY+AATGK NE+ EYKSPRDQDGHGTHTAATVAG
Sbjct: 181 GACETGRGFEKHHCNRKIVGARVFYRGYEAATGKINEKNEYKSPRDQDGHGTHTAATVAG 240

Query: 275 SPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSDILSAVDRAVADGVNVLSISL 334
           SPV GA+LLGYAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGV+VLSISL
Sbjct: 241 SPVRGANLLGYAYGTARGMAPGARIAAYKVCWTGGCFSSDILSAVDRAVADGVSVLSISL 300

Query: 335 GGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFP 394
           GGGVSSYYRDSL++A FGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFP
Sbjct: 301 GGGVSSYYRDSLAIATFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFP 360

Query: 395 AIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSSSPDPSSLCLEGTLDPHFVAGK 454
           A VKLG GRT+TGVSLY+G+  +  NKQ+PIVYMGSNSSSPDPSSLCLEGTLDPH V+GK
Sbjct: 361 ADVKLGTGRTLTGVSLYKGQRFLSPNKQYPIVYMGSNSSSPDPSSLCLEGTLDPHIVSGK 420

Query: 455 IVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAIGEREGKAIKQ 514
           IVICDRGISPRVQKG VVK+AGGIGMIL+NTAANGEELVADCHL+PA+A+GE EGKAIK 
Sbjct: 421 IVICDRGISPRVQKGQVVKDAGGIGMILTNTAANGEELVADCHLLPALAVGEMEGKAIKH 480

Query: 515 YALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTG 574
           YALT+R+ATATL FLGTRLG++PSPVVAAFSSRGPNFLT EILKPD+VAPGVNILAAWTG
Sbjct: 481 YALTSRKATATLAFLGTRLGIRPSPVVAAFSSRGPNFLTFEILKPDMVAPGVNILAAWTG 540

Query: 575 KTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHD 634
           + GPSSL TD RRVKFNILSGTSMSCPHVSG+AAL+K++HP+WSP+AIKSALMTTAYVHD
Sbjct: 541 ELGPSSLQTDHRRVKFNILSGTSMSCPHVSGIAALLKARHPEWSPAAIKSALMTTAYVHD 600

Query: 635 NTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLSPTQLKV 694
           NT+ PLKD++ A+ S+PYDHGAGHINP KALDPGLVY+I+ QDYF+FLCTQ L+  QLKV
Sbjct: 601 NTHNPLKDAAEAAISTPYDHGAGHINPLKALDPGLVYDIEAQDYFEFLCTQKLTTMQLKV 660

Query: 695 FSKYSNRTCRGLLPNPGDLNYPAISAVFPEKTTVTS-LTLHRTVTNVGPATSSYHAVVSP 754
           F KYSNR C   L + GDLNYPAIS VFPE TT  S LTLHRTVTNVGP  S+YH VVS 
Sbjct: 661 FGKYSNRFCHHTLASAGDLNYPAISVVFPEDTTAISVLTLHRTVTNVGPPISNYHVVVSQ 720

Query: 755 FKGATVKVEPESLNFTRRYEKVSYRITFVTKKRQSMPEFGGLIWKDGSHKVRSPIVITWL 805
           FKGATVKV+P+SLNFTR+ +K+SY+ITF TK  Q++PEFGGL+WKDG HKVRSPIVITW+
Sbjct: 721 FKGATVKVDPKSLNFTRKNQKLSYKITFTTKSPQTVPEFGGLVWKDGVHKVRSPIVITWI 775

BLAST of Csa3G178520.1 vs. TrEMBL
Match: M5XPU8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa1027166mg PE=4 SV=1)

HSP 1 Score: 1243.4 bits (3216), Expect = 0.0e+00
Identity = 597/777 (76.83%), Postives = 685/777 (88.16%), Query Frame = 1

Query: 35  MADS-PLKCLCFLL-----FLDSFLLSSALFLKTYVVQMDRSAMPDSFTNHFEWYSNVLT 94
           MAD  P+K L  +L     F  +F   +    KTY+VQMD+SA P+SFTNH +WYS+ + 
Sbjct: 1   MADQKPVKWLVLILTNCLFFSIAFSAKTQFAHKTYIVQMDKSAKPESFTNHLDWYSSKVN 60

Query: 95  NVVLDLQREGNGGGGEERIIYGYHNVFHGVAARLSEEEVEKLEEEDGVVAIFPEMKYELH 154
           ++V   + E +GG  +ER+IY Y N FHGVAARLSEEE E+L+E+DGV+AIFP+ KY+LH
Sbjct: 61  SIVFKPENEEDGGHDQERVIYTYQNAFHGVAARLSEEEAERLQEQDGVLAIFPDTKYQLH 120

Query: 155 TTRSPRFLGLEPADSNSA-WSQQIADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWKGE 214
           TTRSP FLGLEP DS +  WSQ++ DHDV+VGVLDTG+WPES SF+D GMSPVPA+WKG 
Sbjct: 121 TTRSPLFLGLEPHDSTTTVWSQRVTDHDVIVGVLDTGVWPESQSFNDTGMSPVPAYWKGA 180

Query: 215 CETGRGFTKQNCNRKIVGARVFYRGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSP 274
           CETGRGF+K NCN+KIVGAR+FY GY+AATGK NEQ E+KSPRDQDGHGTHTAATVAGSP
Sbjct: 181 CETGRGFSKHNCNKKIVGARIFYHGYEAATGKINEQTEFKSPRDQDGHGTHTAATVAGSP 240

Query: 275 VAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSDILSAVDRAVADGVNVLSISLGG 334
           V GA+LLGYA+GTARGMAPGARIAAYKVCW+GGCFSSDILSAVD+AVADGVNVLSISLGG
Sbjct: 241 VRGANLLGYAHGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDKAVADGVNVLSISLGG 300

Query: 335 GVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAI 394
           GVS+YYRDSLS+AAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFP+ 
Sbjct: 301 GVSAYYRDSLSIAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPSS 360

Query: 395 VKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIV 454
           VKLG+GRT+TGVSLY+GR+ +  NKQ+P+VYMG NS+SPDPSSLCLEGTLD   VAGKIV
Sbjct: 361 VKLGNGRTVTGVSLYKGRMMLSTNKQYPVVYMGDNSTSPDPSSLCLEGTLDRRVVAGKIV 420

Query: 455 ICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAIGEREGKAIKQYA 514
           ICDRGISPRVQKG VVK+AGG+GMIL+NTAANGEELVADCHLVPAVA+GE E KAIK YA
Sbjct: 421 ICDRGISPRVQKGQVVKDAGGVGMILANTAANGEELVADCHLVPAVAVGETEAKAIKHYA 480

Query: 515 LTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKT 574
           LT+ RATATL FLGTR GV+PSPVVAAFSSRGPNF++LEILKPD+VAPGVNILAAWTG  
Sbjct: 481 LTSPRATATLAFLGTRTGVRPSPVVAAFSSRGPNFVSLEILKPDVVAPGVNILAAWTGAL 540

Query: 575 GPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNT 634
           GPSSL TD RRVKFNILSGTSMSCPHVSG+AAL+K++HP+WSP+AIKSALMTTAYVHDNT
Sbjct: 541 GPSSLPTDHRRVKFNILSGTSMSCPHVSGIAALLKARHPEWSPAAIKSALMTTAYVHDNT 600

Query: 635 YKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLSPTQLKVFS 694
           +KPL+D+SAA  S+PYDHGAGHINPRKALDPGLVY+I+ QDY +FLCTQ L+P QLKVF+
Sbjct: 601 HKPLQDASAAEASTPYDHGAGHINPRKALDPGLVYDIEAQDYLEFLCTQRLTPMQLKVFT 660

Query: 695 KYSNRTCRGLLPNPGDLNYPAISAVFPEKTTVTSLTLHRTVTNVGPATSSYHAVVSPFKG 754
           KYSNR+C+  L +PGDLNYPAIS VFPE+T V+ LTLHRTVTNVGP  S+YHA+VSPFKG
Sbjct: 661 KYSNRSCKHALASPGDLNYPAISVVFPERTNVSLLTLHRTVTNVGPPVSNYHAIVSPFKG 720

Query: 755 ATVKVEPESLNFTRRYEKVSYRITFVTKKRQSMPEFGGLIWKDGSHKVRSPIVITWL 805
           A VKVEP +L FTR  +K+SY+ITF TK RQ+ PEFGGL+WKDG H+VRSPIV+ WL
Sbjct: 721 AYVKVEPRTLKFTRANQKLSYKITFTTKSRQATPEFGGLVWKDGVHRVRSPIVVVWL 777

BLAST of Csa3G178520.1 vs. TrEMBL
Match: A0A0D2ST22_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_006G065800 PE=4 SV=1)

HSP 1 Score: 1231.1 bits (3184), Expect = 0.0e+00
Identity = 600/779 (77.02%), Postives = 672/779 (86.26%), Query Frame = 1

Query: 35  MADSPLK--------CLCFLLFLDSFLLSSALFLKTYVVQMDRSAMPDSFTNHFEWYSNV 94
           MA++P+K        CLCF   L     S+ L  KTY+VQM +SAMP SF++  EWYS+ 
Sbjct: 1   MAENPVKWLFLILASCLCFAFVLSE---SNLLIKKTYIVQMHKSAMPASFSSPLEWYSSK 60

Query: 95  LTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAARLSEEEVEKLEEEDGVVAIFPEMKYE 154
           L +V+ D Q EG G G E RIIY Y N FHGVAA+L+EEE E+L++EDGVVAI PE KYE
Sbjct: 61  LKSVMSDTQSEGEGDG-ENRIIYSYQNAFHGVAAQLTEEEAERLKQEDGVVAILPETKYE 120

Query: 155 LHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWKG 214
           LHTTRSP FLGLEP +S S WSQ++ADHDV+VGVLDTGIWPES SF+D GM+PVPAHWKG
Sbjct: 121 LHTTRSPMFLGLEPEESTSIWSQKLADHDVIVGVLDTGIWPESASFNDTGMTPVPAHWKG 180

Query: 215 ECETGRGFTKQNCNRKIVGARVFYRGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGS 274
            CETGRGF K +CNRKIVGARVFYRGY+AATGK NE+ EYKSPRDQDGHGTHTAATVAGS
Sbjct: 181 TCETGRGFQKHHCNRKIVGARVFYRGYEAATGKINEKNEYKSPRDQDGHGTHTAATVAGS 240

Query: 275 PVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSDILSAVDRAVADGVNVLSISLG 334
           PV GA+LLGYAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAV DGVNVLSISLG
Sbjct: 241 PVRGANLLGYAYGTARGMAPGARIAAYKVCWTGGCFSSDILSAVDRAVGDGVNVLSISLG 300

Query: 335 GGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPA 394
           GG SSY  DSL++A FGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFP 
Sbjct: 301 GGASSYSHDSLAIATFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPG 360

Query: 395 IVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSSSPDPSSLCLEGTLDPHFVAGKI 454
            VKLG GRTI+GVSLY+GR  +  NKQ+P+VYMGSNSSSP+PSSLCLEGTLDPH V+GKI
Sbjct: 361 SVKLGSGRTISGVSLYKGRRLLQANKQYPLVYMGSNSSSPNPSSLCLEGTLDPHVVSGKI 420

Query: 455 VICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAIGEREGKAIKQY 514
           VICDRGI+PRVQKG VVK+AGG+GMIL+NTAANGEELVADCHL+PAVA+GE EGKAIK Y
Sbjct: 421 VICDRGINPRVQKGQVVKDAGGVGMILTNTAANGEELVADCHLLPAVAVGEMEGKAIKHY 480

Query: 515 ALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGK 574
           ALTN + TATL FLGTRLGV+PSPVVAAFSSRGPNFLTLEILKPD+VAPGVNILAAWTG+
Sbjct: 481 ALTNGKPTATLAFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDVVAPGVNILAAWTGE 540

Query: 575 TGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDN 634
            GPSSL TD RRV+FNILSGTSMSCPHVSG+AALIK++HPDWSP+A+KSALMTTAYVHDN
Sbjct: 541 LGPSSLPTDHRRVRFNILSGTSMSCPHVSGIAALIKARHPDWSPAAVKSALMTTAYVHDN 600

Query: 635 TYKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLSPTQLKVF 694
            + PL+DSS A+ S+PYDHGAGHINP KALDPGL+Y+I  QDYF+FLCTQ L+  QLK F
Sbjct: 601 IHNPLQDSSTAAASTPYDHGAGHINPLKALDPGLIYDISAQDYFEFLCTQKLTAMQLKAF 660

Query: 695 SKYSNRTC-RGLLPNPGDLNYPAISAVFPEKTTVTSLTLHRTVTNVGPATSSYHAVVSPF 754
           SK+SN +C    L  PGDLNYPAIS VFPE T +++LTLHRTVTNVGP  S YH VVSPF
Sbjct: 661 SKHSNMSCHHNTLATPGDLNYPAISVVFPEDTAISTLTLHRTVTNVGPPASHYHVVVSPF 720

Query: 755 KGATVKVEPESLNFTRRYEKVSYRITFVTKKRQSMPEFGGLIWKDGSHKVRSPIVITWL 805
           KG T+KVEP++LNFTRR +K+SY+I+F  K  Q+MPEFGGL WKDG HKVRSPI ITWL
Sbjct: 721 KGVTIKVEPKTLNFTRRNQKLSYKISFTRKSPQTMPEFGGLAWKDGVHKVRSPIAITWL 775

BLAST of Csa3G178520.1 vs. TrEMBL
Match: B9R9K9_RICCO (Cucumisin, putative OS=Ricinus communis GN=RCOM_1498530 PE=4 SV=1)

HSP 1 Score: 1228.8 bits (3178), Expect = 0.0e+00
Identity = 595/776 (76.68%), Postives = 680/776 (87.63%), Query Frame = 1

Query: 35  MADSPLKCLCFLLFLDSFL-----LSSALFLKTYVVQMDRSAMPDSFTNHFEWYSNVLTN 94
           M+  PL+ L F++   S+L     LS  L  +TY++QMD+ A P+SF+NH EWYS+ + +
Sbjct: 1   MSGGPLRWLFFIV--TSYLAFIVVLSYPLNRQTYIIQMDKYAKPESFSNHLEWYSSKVQS 60

Query: 95  VVLDLQREGNGGGGEERIIYGYHNVFHGVAARLSEEEVEKLEEEDGVVAIFPEMKYELHT 154
           V+   + E +    +ERIIY Y  VFHGVAA+LSEEE ++LEE DGVVAIFPE KY++HT
Sbjct: 61  VLSKSEHEADTDN-DERIIYSYQTVFHGVAAKLSEEEAKRLEEADGVVAIFPETKYQIHT 120

Query: 155 TRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWKGECE 214
           TRSP FLGLEP DS S WSQ IADHDV+VGVLDTGIWPES SF+D GM+ VPAHWKG CE
Sbjct: 121 TRSPMFLGLEPQDSTSVWSQTIADHDVIVGVLDTGIWPESASFNDTGMTTVPAHWKGTCE 180

Query: 215 TGRGFTKQNCNRKIVGARVFYRGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVA 274
           TGRGF K +CN+KIVGARVFY+GY+ ATGK NEQ EYKSPRDQDGHGTHTAATVAGSPV 
Sbjct: 181 TGRGFGKHHCNKKIVGARVFYKGYEVATGKINEQNEYKSPRDQDGHGTHTAATVAGSPVH 240

Query: 275 GASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSDILSAVDRAVADGVNVLSISLGGGV 334
            A+LLGYAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAV+DGVNVLSISLGGGV
Sbjct: 241 DANLLGYAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVSDGVNVLSISLGGGV 300

Query: 335 SSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVK 394
           SSYYRDSLS+AAFGAMEMG+FVSCSAGNGGPDP SLTNVSPWITTVGASTMDRDFPA V 
Sbjct: 301 SSYYRDSLSIAAFGAMEMGIFVSCSAGNGGPDPASLTNVSPWITTVGASTMDRDFPATVH 360

Query: 395 LGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVIC 454
           LG GRT+TGVSLY+GR T+  NKQ+P+VYMGSNSSSPDPSSLCLEGTL+PH VAGKIVIC
Sbjct: 361 LGTGRTLTGVSLYKGRRTLLTNKQYPLVYMGSNSSSPDPSSLCLEGTLNPHIVAGKIVIC 420

Query: 455 DRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAIGEREGKAIKQYALT 514
           DRGISPRVQKG V K+AG +GMIL+NTAANGEELVADCHL PAV++GEREGK IK YALT
Sbjct: 421 DRGISPRVQKGQVAKDAGAVGMILTNTAANGEELVADCHLFPAVSVGEREGKLIKHYALT 480

Query: 515 NRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGP 574
            R A+ATL FLGT++G++PSPVVAAFSSRGPNFL+LEILKPD+VAPGVNI+AAWTG+TGP
Sbjct: 481 RRNASATLAFLGTKVGIRPSPVVAAFSSRGPNFLSLEILKPDVVAPGVNIIAAWTGETGP 540

Query: 575 SSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYK 634
           SSL TD RRV+FNILSGTSMSCPHVSG+AAL+K++HP+WSP+AIKSALMTTAYVHDNT K
Sbjct: 541 SSLPTDHRRVRFNILSGTSMSCPHVSGIAALLKARHPEWSPAAIKSALMTTAYVHDNTQK 600

Query: 635 PLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLSPTQLKVFSKY 694
           PL+D+S  +PSSPYDHGAGHINP KALDPGL+Y+I+ QDYF+FLCTQ LS TQL+VF KY
Sbjct: 601 PLQDASTDAPSSPYDHGAGHINPLKALDPGLIYDIEAQDYFEFLCTQRLSITQLRVFGKY 660

Query: 695 SNRTCRGLLPNPGDLNYPAISAVFPEKTTVTSLTLHRTVTNVGPATSSYHAVVSPFKGAT 754
           +NRTC+  L +PGDLNYPAISAVF +  T++SLTLHRTVTNVGP TS+YHAVVS FKGAT
Sbjct: 661 ANRTCQKSLLSPGDLNYPAISAVFTDSNTISSLTLHRTVTNVGPPTSTYHAVVSRFKGAT 720

Query: 755 VKVEPESLNFTRRYEKVSYRITFVTKKRQSMPEFGGLIWKDGSHKVRSPIVITWLS 806
           VK+EP++L FT + +K+SYRITF  K RQ MPEFGGL+WKDG HKVRSPIV+TWL+
Sbjct: 721 VKIEPKTLKFTAKNQKLSYRITFTAKSRQIMPEFGGLVWKDGVHKVRSPIVLTWLT 773

BLAST of Csa3G178520.1 vs. TrEMBL
Match: B9ICZ0_POPTR (Subtilase family protein OS=Populus trichocarpa GN=POPTR_0015s14110g PE=4 SV=2)

HSP 1 Score: 1228.8 bits (3178), Expect = 0.0e+00
Identity = 594/778 (76.35%), Postives = 679/778 (87.28%), Query Frame = 1

Query: 35  MADSPLKCLCFLLFLDSFL-------LSSALFLKTYVVQMDRSAMPDSFTNHFEWYSNVL 94
           M  +P+K L F+L   S+L       +++ L  KTY+VQMD+SA P+ FT+H EWYS+ +
Sbjct: 1   MFGNPVKWLFFIL--TSYLALNVVVSMNTLLTRKTYIVQMDKSAKPEYFTSHLEWYSSKV 60

Query: 95  TNVVLDLQREGNGGGGEERIIYGYHNVFHGVAARLSEEEVEKLEEEDGVVAIFPEMKYEL 154
            +V+ + Q EG+    E+RIIY Y   FHGVAA+L+EEE  +LEE DGVVAIFPE KY+L
Sbjct: 61  QSVLSEPQGEGDADE-EDRIIYSYETAFHGVAAKLNEEEAARLEEADGVVAIFPETKYQL 120

Query: 155 HTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWKGE 214
           HTTRSP FL LEP DS S WS+++ADHDV+VGVLDTGIWPES+SF+D G++ VP HWKG 
Sbjct: 121 HTTRSPMFLRLEPEDSTSVWSEKLADHDVIVGVLDTGIWPESESFNDTGITAVPVHWKGI 180

Query: 215 CETGRGFTKQNCNRKIVGARVFYRGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSP 274
           CETGR F K +CNRKIVGARVFYRGY+AATGK NEQ EYKSPRDQDGHGTHTAATVAGSP
Sbjct: 181 CETGRAFQKHHCNRKIVGARVFYRGYEAATGKINEQNEYKSPRDQDGHGTHTAATVAGSP 240

Query: 275 VAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSDILSAVDRAVADGVNVLSISLGG 334
           V GA+LLGYAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGG
Sbjct: 241 VRGANLLGYAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGG 300

Query: 335 GVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAI 394
           GVSSYYRDSLS+AAFGAMEMGVFVSCSAGNGGP P SLTNVSPWITTVGAS+MDRDFPA 
Sbjct: 301 GVSSYYRDSLSIAAFGAMEMGVFVSCSAGNGGPSPASLTNVSPWITTVGASSMDRDFPAT 360

Query: 395 VKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIV 454
             +G G+TI+GVSLYRG+  +   KQ+P+VYMGSNSSSPDPSSLCLEGTL+P  V+GKIV
Sbjct: 361 AMIGTGKTISGVSLYRGQRILSTRKQYPLVYMGSNSSSPDPSSLCLEGTLNPRVVSGKIV 420

Query: 455 ICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAIGEREGKAIKQYA 514
           ICDRGI+PRVQKG V K AG +GMILSNTAANGEELVADCHL+PAVA+GE+EGK IK YA
Sbjct: 421 ICDRGITPRVQKGQVAKEAGAVGMILSNTAANGEELVADCHLLPAVAVGEKEGKLIKTYA 480

Query: 515 LTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKT 574
           LT++ ATATL FLGTRLG+KPSPVVAAFSSRGPNFLTLEILKPD++APGVNILAAWTG  
Sbjct: 481 LTSQNATATLAFLGTRLGIKPSPVVAAFSSRGPNFLTLEILKPDVLAPGVNILAAWTGDL 540

Query: 575 GPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNT 634
           GPSSL TD RRVKFNILSGTSMSCPHVSG+AAL+K++HP+WSP+AIKSALMTTAYVHDNT
Sbjct: 541 GPSSLPTDHRRVKFNILSGTSMSCPHVSGIAALLKARHPEWSPAAIKSALMTTAYVHDNT 600

Query: 635 YKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLSPTQLKVFS 694
           + PLKD+SA +PS+PYDHGAGHINP KALDPGL+Y+I+PQDYFDFLCTQ L+PTQLKVF 
Sbjct: 601 HNPLKDASATTPSTPYDHGAGHINPMKALDPGLIYDIEPQDYFDFLCTQKLTPTQLKVFG 660

Query: 695 KYSNRTCRGLLPNPGDLNYPAISAVFPEKTTVTSLTLHRTVTNVGPATSSYHAVVSPFKG 754
           KY+NR+CR  L NPGDLNYPAIS VFP+ T++  LTLHRTVTNVG  TS YHAV+SPFKG
Sbjct: 661 KYANRSCRHSLANPGDLNYPAISVVFPDDTSIKVLTLHRTVTNVGLPTSKYHAVISPFKG 720

Query: 755 ATVKVEPESLNFTRRYEKVSYRITFVTKKRQSMPEFGGLIWKDGSHKVRSPIVITWLS 806
           ATVKVEPE LNFT + +K+SY+I F T+ RQ++PEFGGL+WKDG+HKVRSP+VITWL+
Sbjct: 721 ATVKVEPEILNFTMKNQKLSYKIIFTTRTRQTIPEFGGLVWKDGAHKVRSPVVITWLT 775

BLAST of Csa3G178520.1 vs. TAIR10
Match: AT5G51750.1 (AT5G51750.1 subtilase 1.3)

HSP 1 Score: 1157.5 bits (2993), Expect = 0.0e+00
Identity = 551/760 (72.50%), Postives = 651/760 (85.66%), Query Frame = 1

Query: 46  LLFLDSFLLSSALFLKTYVVQMDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEE 105
           L+FL +   +     KTYV+ MD+SAMP  +TNH +WYS+ + +V     +E  G     
Sbjct: 20  LIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNN-- 79

Query: 106 RIIYGYHNVFHGVAARLSEEEVEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNS 165
           RI+Y Y   FHG+AA+L++EE E+LEEEDGVVA+ PE +YELHTTRSP FLGLE  +S  
Sbjct: 80  RILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESER 139

Query: 166 AWSQQIADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVG 225
            W++++ DHDVVVGVLDTGIWPES+SF+D GMSPVPA W+G CETG+ F K+NCNRKIVG
Sbjct: 140 VWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVG 199

Query: 226 ARVFYRGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMA 285
           ARVFYRGY+AATGK +E+LEYKSPRD+DGHGTHTAATVAGSPV GA+L G+AYGTARGMA
Sbjct: 200 ARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMA 259

Query: 286 PGARIAAYKVCWIGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAM 345
             AR+AAYKVCW+GGCFSSDILSAVD+AVADGV VLSISLGGGVS+Y RDSLS+A FGAM
Sbjct: 260 QKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAM 319

Query: 346 EMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGR 405
           EMGVFVSCSAGNGGPDP+SLTNVSPWITTVGASTMDRDFPA VK+G  RT  GVSLY+GR
Sbjct: 320 EMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGR 379

Query: 406 ITIPENKQFPIVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKN 465
             +P+NKQ+P+VY+G N+SSPDP+S CL+G LD   VAGKIVICDRG++PRVQKG VVK 
Sbjct: 380 TVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKR 439

Query: 466 AGGIGMILSNTAANGEELVADCHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLG 525
           AGGIGM+L+NTA NGEELVAD H++PAVA+GE+EGK IKQYA+T+++ATA+L  LGTR+G
Sbjct: 440 AGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIG 499

Query: 526 VKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILS 585
           +KPSPVVAAFSSRGPNFL+LEILKPDL+APGVNILAAWTG   PSSL++D RRVKFNILS
Sbjct: 500 IKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILS 559

Query: 586 GTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDH 645
           GTSMSCPHVSGVAALIKS+HPDWSP+AIKSALMTTAYVHDN +KPL D+S A+PSSPYDH
Sbjct: 560 GTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDH 619

Query: 646 GAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLSPTQLKVFSKYSNRTCR-GLLPNPGDL 705
           GAGHI+P +A DPGLVY+I PQ+YF+FLCTQDLSP+QLKVF+K+SNRTC+  L  NPG+L
Sbjct: 620 GAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNL 679

Query: 706 NYPAISAVFPEKTTVTSLTLHRTVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYE 765
           NYPAISA+FPE T V ++TL RTVTNVGP  SSY   VSPFKGA+V V+P++LNFT +++
Sbjct: 680 NYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQ 739

Query: 766 KVSYRITFVTKKRQSMPEFGGLIWKDGSHKVRSPIVITWL 805
           K+SY +TF T+ R   PEFGGL+WK  +HKVRSP++ITWL
Sbjct: 740 KLSYTVTFRTRFRMKRPEFGGLVWKSTTHKVRSPVIITWL 777

BLAST of Csa3G178520.1 vs. TAIR10
Match: AT5G67360.1 (AT5G67360.1 Subtilase family protein)

HSP 1 Score: 805.4 bits (2079), Expect = 3.1e-233
Identity = 411/772 (53.24%), Postives = 523/772 (67.75%), Query Frame = 1

Query: 51  SFLLSSALFLK----------------TYVVQMDRSAMPDSFTNHFEWYSNVLTNVVLDL 110
           SFL S+A FL                 TY+V M +S MP SF  H  WY + L ++    
Sbjct: 4   SFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSI---- 63

Query: 111 QREGNGGGGEERIIYGYHNVFHGVAARLSEEEVEKLEEEDGVVAIFPEMKYELHTTRSPR 170
                       ++Y Y N  HG + RL++EE + L  + GV+++ PE +YELHTTR+P 
Sbjct: 64  -------SDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPL 123

Query: 171 FLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWKGECETGRGF 230
           FLGL+   ++  + +  +  DVVVGVLDTG+WPES S+ D G  P+P+ WKG CE G  F
Sbjct: 124 FLGLDEHTAD-LFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNF 183

Query: 231 TKQNCNRKIVGARVFYRGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL 290
           T   CNRK++GAR F RGY++  G  +E  E +SPRD DGHGTHT++T AGS V GASLL
Sbjct: 184 TASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLL 243

Query: 291 GYAYGTARGMAPGARIAAYKVCWIGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR 350
           GYA GTARGMAP AR+A YKVCW+GGCFSSDIL+A+D+A+AD VNVLS+SLGGG+S YYR
Sbjct: 244 GYASGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYR 303

Query: 351 DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR 410
           D +++ AF AME G+ VSCSAGN GP   SL+NV+PWITTVGA T+DRDFPA+  LG+G+
Sbjct: 304 DGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGK 363

Query: 411 TITGVSLYRGRITIPENKQFPIVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGIS 470
             TGVSL++G   +P+ K  P +Y G N+S+    +LC+ GTL P  V GKIV+CDRGI+
Sbjct: 364 NFTGVSLFKGE-ALPD-KLLPFIYAG-NASNATNGNLCMTGTLIPEKVKGKIVMCDRGIN 423

Query: 471 PRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAIGEREGKAIKQYALTNRRAT 530
            RVQKG VVK AGG+GMIL+NTAANGEELVAD HL+PA  +GE+ G  I+ Y  T+   T
Sbjct: 424 ARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPT 483

Query: 531 ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT 590
           A++  LGT +GVKPSPVVAAFSSRGPN +T  ILKPDL+APGVNILAAWTG  GP+ L +
Sbjct: 484 ASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLAS 543

Query: 591 DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDS 650
           D+RRV+FNI+SGTSMSCPHVSG+AAL+KS HP+WSP+AI+SALMTTAY      KPL D 
Sbjct: 544 DSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDI 603

Query: 651 SAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLSPTQLKVFSKYSNRTC 710
           +   PS+P+DHGAGH++P  A +PGL+Y++  +DY  FLC  + +  Q++  S+  N TC
Sbjct: 604 ATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSR-RNYTC 663

Query: 711 -RGLLPNPGDLNYPAISAVFPEKTTVTSLTLHRTVTNVGPATSSYHAVVSPFKGATVKVE 770
                 +  DLNYP+ +        V +    RTVT+VG A +    V S   G  + VE
Sbjct: 664 DPSKSYSVADLNYPSFAV---NVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVE 723

Query: 771 PESLNFTRRYEKVSYRITFV--TKKRQSMPEFGGLIWKDGSHKVRSPIVITW 804
           P  LNF    EK SY +TF   + K      FG + W DG H V SP+ I+W
Sbjct: 724 PAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Csa3G178520.1 vs. TAIR10
Match: AT2G05920.1 (AT2G05920.1 Subtilase family protein)

HSP 1 Score: 768.1 bits (1982), Expect = 5.5e-222
Identity = 390/760 (51.32%), Postives = 509/760 (66.97%), Query Frame = 1

Query: 52  FLLSSALFLKTYVVQMDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGY 111
           FLL      KTY+++++ S  P+SF  H +WY++ L +              E  ++Y Y
Sbjct: 19  FLLLHTTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNS--------------ESSLLYTY 78

Query: 112 HNVFHGVAARLSEEEVEKL-EEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQ 171
              FHG +A L   E + L    + ++ IF +  Y LHTTR+P FLGL            
Sbjct: 79  TTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLN--SEFGVHDLG 138

Query: 172 IADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFY 231
            + + V++GVLDTG+WPES SFDD  M  +P+ WKGECE+G  F  + CN+K++GAR F 
Sbjct: 139 SSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFS 198

Query: 232 RGYQAATGK-FNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGAR 291
           +G+Q A+G  F+ + E  SPRD DGHGTHT+ T AGS V  AS LGYA GTARGMA  AR
Sbjct: 199 KGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRAR 258

Query: 292 IAAYKVCWIGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGV 351
           +A YKVCW  GCF SDIL+A+DRA+ DGV+VLS+SLGGG + YYRD++++ AF AME GV
Sbjct: 259 VATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGV 318

Query: 352 FVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIP 411
           FVSCSAGN GP   S+ NV+PW+ TVGA T+DRDFPA   LG+G+ +TGVSLY G     
Sbjct: 319 FVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSG--VGM 378

Query: 412 ENKQFPIVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGI 471
             K   +VY   NSSS   S+LCL G+LD   V GKIV+CDRG++ RV+KG VV++AGG+
Sbjct: 379 GTKPLELVYNKGNSSS---SNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGL 438

Query: 472 GMILSNTAANGEELVADCHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPS 531
           GMI++NTAA+GEELVAD HL+PA+A+G++ G  +++Y  ++ + TA L F GT L VKPS
Sbjct: 439 GMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPS 498

Query: 532 PVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSM 591
           PVVAAFSSRGPN +T EILKPD++ PGVNILA W+   GP+ L  D+RR +FNI+SGTSM
Sbjct: 499 PVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSM 558

Query: 592 SCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGH 651
           SCPH+SG+A L+K+ HP+WSPSAIKSALMTTAYV DNT  PL D++  S S+PY HG+GH
Sbjct: 559 SCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGH 618

Query: 652 INPRKALDPGLVYEIQPQDYFDFLCTQDLSPTQLKVFSKYSNRTCRGLLPNPGDLNYPAI 711
           ++P+KAL PGLVY+I  ++Y  FLC+ D +   +    K  +  C     +PG LNYP+ 
Sbjct: 619 VDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSF 678

Query: 712 SAVFPEKTTVTSLTLHRTVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYR 771
           S +F  K  V      R VTNVG A+S Y   V+      + V+P  L+F    EK  Y 
Sbjct: 679 SVLFGGKRVV---RYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYT 738

Query: 772 ITFVTKKRQSM---PEFGGLIWKDGSHKVRSPIVITWLSF 807
           +TFV+KK  SM    EFG + W +  H+VRSP+  +W  F
Sbjct: 739 VTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSWNRF 754

BLAST of Csa3G178520.1 vs. TAIR10
Match: AT4G34980.1 (AT4G34980.1 subtilisin-like serine protease 2)

HSP 1 Score: 756.5 bits (1952), Expect = 1.7e-218
Identity = 395/782 (50.51%), Postives = 519/782 (66.37%), Query Frame = 1

Query: 35  MADSPLKCLCFLLF-LDSFLLSSALFLKTYVVQMDRSAMPDSFTNHFEWYSNVLTNVVLD 94
           MA S +  L FL F   SF  S A   KT++ ++D  +MP  F  H+ WYS         
Sbjct: 1   MASSTIVLLLFLSFPFISFAASQAA--KTFIFRIDGGSMPSIFPTHYHWYSTEFAE---- 60

Query: 95  LQREGNGGGGEERIIYGYHNVFHGVAARLSEEEVEKLEEEDGVVAIFPEMKYELHTTRSP 154
                     E RI++ YH VFHG +A ++ +E + L     V+A+F + + ELHTTRSP
Sbjct: 61  ----------ESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSP 120

Query: 155 RFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWKGECETGRG 214
           +FLGL+  +    WS+     DV++GV DTGIWPE  SF D  + P+P  W+G CE+G  
Sbjct: 121 QFLGLQ--NQKGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGAR 180

Query: 215 FTKQNCNRKIVGARVFYRGYQAAT-GKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGAS 274
           F+ +NCNRKI+GAR F +G QAA  G  N+ +E+ SPRD DGHGTHT++T AG     AS
Sbjct: 181 FSPRNCNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKAS 240

Query: 275 LLGYAYGTARGMAPGARIAAYKVCWI-GGCFSSDILSAVDRAVADGVNVLSISLGGG--- 334
           + GYA G A+G+AP ARIAAYKVCW   GC  SDIL+A D AV DGV+V+SIS+GGG   
Sbjct: 241 MSGYASGVAKGVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGI 300

Query: 335 VSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIV 394
            S YY D +++ ++GA   G+FVS SAGN GP+ +S+TN++PW+TTVGAST+DR+FPA  
Sbjct: 301 TSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADA 360

Query: 395 KLGDGRTITGVSLYRGRITIPEN-KQFPIVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIV 454
            LGDG  + GVSLY G   +P N + FP+VY G +  S   +SLC+E TLDP  V GKIV
Sbjct: 361 ILGDGHRLRGVSLYAG---VPLNGRMFPVVYPGKSGMSS--ASLCMENTLDPKQVRGKIV 420

Query: 455 ICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAIGEREGKAIKQYA 514
           ICDRG SPRV KG+VVK AGG+GMIL+N A+NGE LV D HL+PA A+G  EG  IK YA
Sbjct: 421 ICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYA 480

Query: 515 LTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKT 574
            ++    A++ F GT +G+KP+PV+A+FS RGPN L+ EILKPDL+APGVNILAAWT   
Sbjct: 481 SSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAV 540

Query: 575 GPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNT 634
           GP+ L +D R+ +FNILSGTSM+CPHVSG AAL+KS HPDWSP+ I+SA+MTT  + DN+
Sbjct: 541 GPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNS 600

Query: 635 YKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLSPTQLKVFS 694
            + L D S    ++PYD+G+GH+N  +A++PGLVY+I   DY  FLC+    P  ++V +
Sbjct: 601 NRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVIT 660

Query: 695 KYSNRTCRGLLPNPGDLNYPAISAVFP-EKTTVTSLTLHRTVTNVGPATSSYHAVVSPFK 754
           +   R      P+PG+LNYP+I+AVFP  +  + S T+ RT TNVG A + Y A +   +
Sbjct: 661 RTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPR 720

Query: 755 GATVKVEPESLNFTRRYEKVSYRITFVTKKR-----QSMPEFGGLIWKD-GSHKVRSPIV 803
           G TV V+P  L FT   ++ SY +T     R     ++   FG + W D G H VRSPIV
Sbjct: 721 GVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIV 759

BLAST of Csa3G178520.1 vs. TAIR10
Match: AT3G14240.1 (AT3G14240.1 Subtilase family protein)

HSP 1 Score: 754.6 bits (1947), Expect = 6.3e-218
Identity = 379/757 (50.07%), Postives = 513/757 (67.77%), Query Frame = 1

Query: 62  TYVVQMDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAAR 121
           TY+V +D  A P  F  HF WY++ L ++                II+ Y  VFHG +AR
Sbjct: 27  TYIVHVDHEAKPSIFPTHFHWYTSSLASLT----------SSPPSIIHTYDTVFHGFSAR 86

Query: 122 LSEEEVEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVL 181
           L+ ++  +L +   V+++ PE    LHTTRSP FLGL   D      +     D+V+GV+
Sbjct: 87  LTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVI 146

Query: 182 DTGIWPESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGKFN 241
           DTG+WPE  SFDD G+ PVP  WKG+C   + F +  CNRK+VGAR F  GY+A  GK N
Sbjct: 147 DTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMN 206

Query: 242 EQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGC 301
           E  E++SPRD DGHGTHTA+  AG  V  AS LGYA+G A GMAP AR+AAYKVCW  GC
Sbjct: 207 ETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGC 266

Query: 302 FSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPD 361
           + SDIL+A D AVADGV+V+S+S+GG V  YY D++++ AFGA++ G+FVS SAGNGGP 
Sbjct: 267 YDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPG 326

Query: 362 PVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGS 421
            +++TNV+PW+TTVGA T+DRDFPA VKLG+G+ I+GVS+Y G    P  + +P+VY GS
Sbjct: 327 ALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDP-GRMYPLVYGGS 386

Query: 422 NSSSPD-PSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANG 481
                   SSLCLEG+LDP+ V GKIV+CDRGI+ R  KG +V+  GG+GMI++N   +G
Sbjct: 387 LLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDG 446

Query: 482 EELVADCHLVPAVAIGEREGKAIKQY------ALTNRRATATLGFLGTRLGVKPSPVVAA 541
           E LVADCH++PA ++G   G  I++Y      + +++  TAT+ F GTRLG++P+PVVA+
Sbjct: 447 EGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVAS 506

Query: 542 FSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHV 601
           FS+RGPN  T EILKPD++APG+NILAAW  + GPS +T+D RR +FNILSGTSM+CPHV
Sbjct: 507 FSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHV 566

Query: 602 SGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRK 661
           SG+AAL+K+ HPDWSP+AI+SAL+TTAY  DN+ +P+ D S  + SS  D+G+GH++P K
Sbjct: 567 SGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTK 626

Query: 662 ALDPGLVYEIQPQDYFDFLCTQDLSPTQLKVFSKYSNRTCRGL--LPNPGDLNYPAISAV 721
           A+DPGLVY+I   DY +FLC  + + T +   ++     C G     + G+LNYP+ S V
Sbjct: 627 AMDPGLVYDITSYDYINFLCNSNYTRTNIVTITR-RQADCDGARRAGHVGNLNYPSFSVV 686

Query: 722 FPEKTTVTSLTLH--RTVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRI 781
           F ++   + ++ H  RTVTNVG + S Y   + P +G TV VEPE L+F R  +K+S+ +
Sbjct: 687 F-QQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVV 746

Query: 782 TFVTKKRQSMP-----EFGGLIWKDGSHKVRSPIVIT 803
              T + +  P     E G ++W DG   V SP+V+T
Sbjct: 747 RVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of Csa3G178520.1 vs. NCBI nr
Match: gi|778679387|ref|XP_004148149.2| (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis sativus])

HSP 1 Score: 1634.8 bits (4232), Expect = 0.0e+00
Identity = 807/807 (100.00%), Postives = 807/807 (100.00%), Query Frame = 1

Query: 1   MKQPLFFITLTSILTTPPFPLHFSQTHSFPLFPKMADSPLKCLCFLLFLDSFLLSSALFL 60
           MKQPLFFITLTSILTTPPFPLHFSQTHSFPLFPKMADSPLKCLCFLLFLDSFLLSSALFL
Sbjct: 1   MKQPLFFITLTSILTTPPFPLHFSQTHSFPLFPKMADSPLKCLCFLLFLDSFLLSSALFL 60

Query: 61  KTYVVQMDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAA 120
           KTYVVQMDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAA
Sbjct: 61  KTYVVQMDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAA 120

Query: 121 RLSEEEVEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGV 180
           RLSEEEVEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGV
Sbjct: 121 RLSEEEVEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGV 180

Query: 181 LDTGIWPESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGKF 240
           LDTGIWPESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGKF
Sbjct: 181 LDTGIWPESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGKF 240

Query: 241 NEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGG 300
           NEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGG
Sbjct: 241 NEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGG 300

Query: 301 CFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGP 360
           CFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGP
Sbjct: 301 CFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGP 360

Query: 361 DPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMG 420
           DPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMG
Sbjct: 361 DPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMG 420

Query: 421 SNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANG 480
           SNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANG
Sbjct: 421 SNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANG 480

Query: 481 EELVADCHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGP 540
           EELVADCHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGP
Sbjct: 481 EELVADCHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGP 540

Query: 541 NFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAAL 600
           NFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAAL
Sbjct: 541 NFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAAL 600

Query: 601 IKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGL 660
           IKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGL
Sbjct: 601 IKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGL 660

Query: 661 VYEIQPQDYFDFLCTQDLSPTQLKVFSKYSNRTCRGLLPNPGDLNYPAISAVFPEKTTVT 720
           VYEIQPQDYFDFLCTQDLSPTQLKVFSKYSNRTCRGLLPNPGDLNYPAISAVFPEKTTVT
Sbjct: 661 VYEIQPQDYFDFLCTQDLSPTQLKVFSKYSNRTCRGLLPNPGDLNYPAISAVFPEKTTVT 720

Query: 721 SLTLHRTVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFVTKKRQSM 780
           SLTLHRTVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFVTKKRQSM
Sbjct: 721 SLTLHRTVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFVTKKRQSM 780

Query: 781 PEFGGLIWKDGSHKVRSPIVITWLSFV 808
           PEFGGLIWKDGSHKVRSPIVITWLSFV
Sbjct: 781 PEFGGLIWKDGSHKVRSPIVITWLSFV 807

BLAST of Csa3G178520.1 vs. NCBI nr
Match: gi|659077296|ref|XP_008439131.1| (PREDICTED: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 1581.2 bits (4093), Expect = 0.0e+00
Identity = 777/807 (96.28%), Postives = 792/807 (98.14%), Query Frame = 1

Query: 1   MKQPLFFITLTSILTTPPFPLHFSQTHSFPLFPKMADSPLKCLCFLLFLDSFLLSSALFL 60
           MKQPLFFITLTSIL TPPFPLHFSQT SFPLFPKMAD PLKCLC LLFLDS LLSSALFL
Sbjct: 1   MKQPLFFITLTSILKTPPFPLHFSQTRSFPLFPKMADFPLKCLCLLLFLDSLLLSSALFL 60

Query: 61  KTYVVQMDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAA 120
           KTYVVQMDRSAMP SFTNHFEWYSNVL+NVV+DL+REGNGGGGEERIIY Y NVFHGVAA
Sbjct: 61  KTYVVQMDRSAMPASFTNHFEWYSNVLSNVVVDLEREGNGGGGEERIIYSYQNVFHGVAA 120

Query: 121 RLSEEEVEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGV 180
           RLSEEEVEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGV
Sbjct: 121 RLSEEEVEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGV 180

Query: 181 LDTGIWPESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGKF 240
           LDTGIWPESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFY GYQAATGKF
Sbjct: 181 LDTGIWPESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYHGYQAATGKF 240

Query: 241 NEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGG 300
           NEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGG
Sbjct: 241 NEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGG 300

Query: 301 CFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGP 360
           CFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGP
Sbjct: 301 CFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGP 360

Query: 361 DPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMG 420
           DPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRT++G SLYRGRITIPENKQFPIVYMG
Sbjct: 361 DPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTMSGASLYRGRITIPENKQFPIVYMG 420

Query: 421 SNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANG 480
           SNSSSPDPSSLCLEGTLDPH VAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNT ANG
Sbjct: 421 SNSSSPDPSSLCLEGTLDPHVVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTVANG 480

Query: 481 EELVADCHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGP 540
           EELVADCHLVPAVA+GE+EGKAIKQYALTNR+ATATLGFLGTRLGVKPSPVVAAFSSRGP
Sbjct: 481 EELVADCHLVPAVAVGEKEGKAIKQYALTNRKATATLGFLGTRLGVKPSPVVAAFSSRGP 540

Query: 541 NFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAAL 600
           NFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAAL
Sbjct: 541 NFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAAL 600

Query: 601 IKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGL 660
           IKSKHPDWSPSAIKSALMTTAY+HDNTYKPL+DSSAASPSSPYDHGAGHINPRKALDPGL
Sbjct: 601 IKSKHPDWSPSAIKSALMTTAYIHDNTYKPLQDSSAASPSSPYDHGAGHINPRKALDPGL 660

Query: 661 VYEIQPQDYFDFLCTQDLSPTQLKVFSKYSNRTCRGLLPNPGDLNYPAISAVFPEKTTVT 720
           VYEIQPQDYFDFLCTQDL+PTQLKVFSKYSNRTCR LLPNPGDLNYPAISAVFPEKT+VT
Sbjct: 661 VYEIQPQDYFDFLCTQDLTPTQLKVFSKYSNRTCRRLLPNPGDLNYPAISAVFPEKTSVT 720

Query: 721 SLTLHRTVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFVTKKRQSM 780
           SLTLHRTVTNVGPATSSYHAVV+PFKGA VKVEPESLNFTRRYEK+SYRITFVTKKR SM
Sbjct: 721 SLTLHRTVTNVGPATSSYHAVVTPFKGAAVKVEPESLNFTRRYEKLSYRITFVTKKRLSM 780

Query: 781 PEFGGLIWKDGSHKVRSPIVITWLSFV 808
           PEFGGLIWKDGSHKVRSPIV+TWLSFV
Sbjct: 781 PEFGGLIWKDGSHKVRSPIVMTWLSFV 807

BLAST of Csa3G178520.1 vs. NCBI nr
Match: gi|645257258|ref|XP_008234331.1| (PREDICTED: subtilisin-like protease [Prunus mume])

HSP 1 Score: 1246.9 bits (3225), Expect = 0.0e+00
Identity = 604/803 (75.22%), Postives = 696/803 (86.67%), Query Frame = 1

Query: 9   TLTSILTTPPFPLHFSQTHSFPLFPKMA-DSPLKCLCFLL-----FLDSFLLSSALFLKT 68
           +L ++L  P F L   +T   P   KMA   P+K L  +L     F  +F   +    KT
Sbjct: 37  SLPTLLFLPYFILQ-GRTVLLPCLMKMAHQKPVKWLVLILTNCLFFSIAFSAKTQFAHKT 96

Query: 69  YVVQMDRSAMPDSFTNHFEWYSNVLTNVVLDLQREGNGGGGEERIIYGYHNVFHGVAARL 128
           Y+VQMD+SA P+SFTNH +WYS+ + ++V   + E +GG  +ER+IY Y N FHGVAARL
Sbjct: 97  YIVQMDKSAKPESFTNHLDWYSSKVNSIVFKPENEEDGGHNQERVIYAYQNAFHGVAARL 156

Query: 129 SEEEVEKLEEEDGVVAIFPEMKYELHTTRSPRFLGLEPADSNS-AWSQQIADHDVVVGVL 188
           SEEE E+L+E+DGV+AIFP+ KY+LHTTRSP FLGLEP DS +  WSQ++ DHDV+VGVL
Sbjct: 157 SEEEAERLQEQDGVLAIFPDTKYQLHTTRSPLFLGLEPHDSTTNVWSQRVTDHDVIVGVL 216

Query: 189 DTGIWPESDSFDDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYRGYQAATGKFN 248
           DTG+WPES SF+D GMSPVPA WKG CETGRGF+K NCN+KIVGAR+FY+GY+AATGK N
Sbjct: 217 DTGVWPESQSFNDTGMSPVPARWKGACETGRGFSKHNCNKKIVGARIFYQGYEAATGKIN 276

Query: 249 EQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGC 308
           EQ E+KSPRDQDGHGTHTAATVAGSPV GA+LLGYA+GTARGMAPGARIAAYKVCW+GGC
Sbjct: 277 EQTEFKSPRDQDGHGTHTAATVAGSPVRGANLLGYAHGTARGMAPGARIAAYKVCWVGGC 336

Query: 309 FSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPD 368
           FSSDILSAVD+AVADGVNVLSISLGGGVS+YYRDSLS+AAFGAMEMGVFVSCSAGNGGPD
Sbjct: 337 FSSDILSAVDKAVADGVNVLSISLGGGVSAYYRDSLSIAAFGAMEMGVFVSCSAGNGGPD 396

Query: 369 PVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGS 428
           PVSLTNVSPWITTVGASTMDRDFP+ VKLG+GRT+TGVSLY+G + +  NKQ+P+VYMG+
Sbjct: 397 PVSLTNVSPWITTVGASTMDRDFPSTVKLGNGRTVTGVSLYKGTMMLSTNKQYPVVYMGN 456

Query: 429 NSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGE 488
           NS+SPDPSSLCLEGTLD   VAGKIVICDRGISPRVQKG VVK+AGG+GMIL+NTAANGE
Sbjct: 457 NSTSPDPSSLCLEGTLDRRVVAGKIVICDRGISPRVQKGQVVKDAGGVGMILANTAANGE 516

Query: 489 ELVADCHLVPAVAIGEREGKAIKQYALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPN 548
           ELVADCHLVPAVA+GE E KAIK YALT+ RATATL FLGTR GV+PSPVVAAFSSRGPN
Sbjct: 517 ELVADCHLVPAVAVGETEAKAIKHYALTSPRATATLAFLGTRTGVRPSPVVAAFSSRGPN 576

Query: 549 FLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALI 608
           F++LEILKPD+VAPGVNILAAWTG  GPSSL TD RRVKFNILSGTSMSCPHVSG+AAL+
Sbjct: 577 FVSLEILKPDVVAPGVNILAAWTGALGPSSLPTDHRRVKFNILSGTSMSCPHVSGIAALL 636

Query: 609 KSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGLV 668
           K++HP+WSP+AIKSALMTTAYVHDNT+KPL+D+SAA  S+PYDHGAGHINPRKALDPGLV
Sbjct: 637 KARHPEWSPAAIKSALMTTAYVHDNTHKPLQDASAAEASTPYDHGAGHINPRKALDPGLV 696

Query: 669 YEIQPQDYFDFLCTQDLSPTQLKVFSKYSNRTCRGLLPNPGDLNYPAISAVFPEKTTVTS 728
           Y+I+ QDY +FLCTQ L+P QLKVF+KYSNR+C+  L +PGDLNYPAIS VFPE+T V+ 
Sbjct: 697 YDIEAQDYLEFLCTQRLTPMQLKVFTKYSNRSCKHSLASPGDLNYPAISVVFPERTNVSL 756

Query: 729 LTLHRTVTNVGPATSSYHAVVSPFKGATVKVEPESLNFTRRYEKVSYRITFVTKKRQSMP 788
           LTLHRTVTNVGP  S+YHA+VSPFKGA VKVEP +L FTR  +K+SY+ITF TK RQ+ P
Sbjct: 757 LTLHRTVTNVGPPVSNYHAIVSPFKGAYVKVEPRTLKFTRANQKLSYKITFTTKSRQATP 816

Query: 789 EFGGLIWKDGSHKVRSPIVITWL 805
           EFGGL+WKDG H+VRSPIVI WL
Sbjct: 817 EFGGLVWKDGVHRVRSPIVIVWL 838

BLAST of Csa3G178520.1 vs. NCBI nr
Match: gi|590672084|ref|XP_007038510.1| (Subtilase 1.3 [Theobroma cacao])

HSP 1 Score: 1243.8 bits (3217), Expect = 0.0e+00
Identity = 607/780 (77.82%), Postives = 688/780 (88.21%), Query Frame = 1

Query: 35  MADSPLK--------CLCFLLFLDSFLLSSALFLKTYVVQMDRSAMPDSFTNHFEWYSNV 94
           M ++P+K        CL F L L +   S+ L  KT++VQMD+SAMP SF++H EWYS+ 
Sbjct: 1   MTENPVKWLFLILTSCLSFALVLSA---SNPLIKKTFIVQMDKSAMPASFSSHLEWYSSK 60

Query: 95  LTNVVL-DLQREGNGGGGEERIIYGYHNVFHGVAARLSEEEVEKLEEEDGVVAIFPEMKY 154
           + +V++ + Q EG+G G  ERIIY Y N FHGVAA+L+E+E E+LEEEDGVVAI PEMKY
Sbjct: 61  VKSVIMSNTQSEGDGDG--ERIIYSYQNAFHGVAAQLTEDEAERLEEEDGVVAILPEMKY 120

Query: 155 ELHTTRSPRFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWK 214
           +LHTTRSP FLGLEP +S S WSQ++ DHDV+VGVLDTGIWPES+SF+D G++PVPAHWK
Sbjct: 121 QLHTTRSPMFLGLEPEESTSIWSQKLTDHDVIVGVLDTGIWPESESFNDTGLAPVPAHWK 180

Query: 215 GECETGRGFTKQNCNRKIVGARVFYRGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAG 274
           G CETGRGF K +CNRKIVGARVFYRGY+AATGK NE+ EYKSPRDQDGHGTHTAATVAG
Sbjct: 181 GACETGRGFEKHHCNRKIVGARVFYRGYEAATGKINEKNEYKSPRDQDGHGTHTAATVAG 240

Query: 275 SPVAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSDILSAVDRAVADGVNVLSISL 334
           SPV GA+LLGYAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGV+VLSISL
Sbjct: 241 SPVRGANLLGYAYGTARGMAPGARIAAYKVCWTGGCFSSDILSAVDRAVADGVSVLSISL 300

Query: 335 GGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFP 394
           GGGVSSYYRDSL++A FGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFP
Sbjct: 301 GGGVSSYYRDSLAIATFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFP 360

Query: 395 AIVKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSSSPDPSSLCLEGTLDPHFVAGK 454
           A VKLG GRT+TGVSLY+G+  +  NKQ+PIVYMGSNSSSPDPSSLCLEGTLDPH V+GK
Sbjct: 361 ADVKLGTGRTLTGVSLYKGQRFLSPNKQYPIVYMGSNSSSPDPSSLCLEGTLDPHIVSGK 420

Query: 455 IVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAIGEREGKAIKQ 514
           IVICDRGISPRVQKG VVK+AGGIGMIL+NTAANGEELVADCHL+PA+A+GE EGKAIK 
Sbjct: 421 IVICDRGISPRVQKGQVVKDAGGIGMILTNTAANGEELVADCHLLPALAVGEMEGKAIKH 480

Query: 515 YALTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTG 574
           YALT+R+ATATL FLGTRLG++PSPVVAAFSSRGPNFLT EILKPD+VAPGVNILAAWTG
Sbjct: 481 YALTSRKATATLAFLGTRLGIRPSPVVAAFSSRGPNFLTFEILKPDMVAPGVNILAAWTG 540

Query: 575 KTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHD 634
           + GPSSL TD RRVKFNILSGTSMSCPHVSG+AAL+K++HP+WSP+AIKSALMTTAYVHD
Sbjct: 541 ELGPSSLQTDHRRVKFNILSGTSMSCPHVSGIAALLKARHPEWSPAAIKSALMTTAYVHD 600

Query: 635 NTYKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLSPTQLKV 694
           NT+ PLKD++ A+ S+PYDHGAGHINP KALDPGLVY+I+ QDYF+FLCTQ L+  QLKV
Sbjct: 601 NTHNPLKDAAEAAISTPYDHGAGHINPLKALDPGLVYDIEAQDYFEFLCTQKLTTMQLKV 660

Query: 695 FSKYSNRTCRGLLPNPGDLNYPAISAVFPEKTTVTS-LTLHRTVTNVGPATSSYHAVVSP 754
           F KYSNR C   L + GDLNYPAIS VFPE TT  S LTLHRTVTNVGP  S+YH VVS 
Sbjct: 661 FGKYSNRFCHHTLASAGDLNYPAISVVFPEDTTAISVLTLHRTVTNVGPPISNYHVVVSQ 720

Query: 755 FKGATVKVEPESLNFTRRYEKVSYRITFVTKKRQSMPEFGGLIWKDGSHKVRSPIVITWL 805
           FKGATVKV+P+SLNFTR+ +K+SY+ITF TK  Q++PEFGGL+WKDG HKVRSPIVITW+
Sbjct: 721 FKGATVKVDPKSLNFTRKNQKLSYKITFTTKSPQTVPEFGGLVWKDGVHKVRSPIVITWI 775

BLAST of Csa3G178520.1 vs. NCBI nr
Match: gi|596038840|ref|XP_007219861.1| (hypothetical protein PRUPE_ppa1027166mg [Prunus persica])

HSP 1 Score: 1243.4 bits (3216), Expect = 0.0e+00
Identity = 597/777 (76.83%), Postives = 685/777 (88.16%), Query Frame = 1

Query: 35  MADS-PLKCLCFLL-----FLDSFLLSSALFLKTYVVQMDRSAMPDSFTNHFEWYSNVLT 94
           MAD  P+K L  +L     F  +F   +    KTY+VQMD+SA P+SFTNH +WYS+ + 
Sbjct: 1   MADQKPVKWLVLILTNCLFFSIAFSAKTQFAHKTYIVQMDKSAKPESFTNHLDWYSSKVN 60

Query: 95  NVVLDLQREGNGGGGEERIIYGYHNVFHGVAARLSEEEVEKLEEEDGVVAIFPEMKYELH 154
           ++V   + E +GG  +ER+IY Y N FHGVAARLSEEE E+L+E+DGV+AIFP+ KY+LH
Sbjct: 61  SIVFKPENEEDGGHDQERVIYTYQNAFHGVAARLSEEEAERLQEQDGVLAIFPDTKYQLH 120

Query: 155 TTRSPRFLGLEPADSNSA-WSQQIADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWKGE 214
           TTRSP FLGLEP DS +  WSQ++ DHDV+VGVLDTG+WPES SF+D GMSPVPA+WKG 
Sbjct: 121 TTRSPLFLGLEPHDSTTTVWSQRVTDHDVIVGVLDTGVWPESQSFNDTGMSPVPAYWKGA 180

Query: 215 CETGRGFTKQNCNRKIVGARVFYRGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSP 274
           CETGRGF+K NCN+KIVGAR+FY GY+AATGK NEQ E+KSPRDQDGHGTHTAATVAGSP
Sbjct: 181 CETGRGFSKHNCNKKIVGARIFYHGYEAATGKINEQTEFKSPRDQDGHGTHTAATVAGSP 240

Query: 275 VAGASLLGYAYGTARGMAPGARIAAYKVCWIGGCFSSDILSAVDRAVADGVNVLSISLGG 334
           V GA+LLGYA+GTARGMAPGARIAAYKVCW+GGCFSSDILSAVD+AVADGVNVLSISLGG
Sbjct: 241 VRGANLLGYAHGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDKAVADGVNVLSISLGG 300

Query: 335 GVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAI 394
           GVS+YYRDSLS+AAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFP+ 
Sbjct: 301 GVSAYYRDSLSIAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPSS 360

Query: 395 VKLGDGRTITGVSLYRGRITIPENKQFPIVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIV 454
           VKLG+GRT+TGVSLY+GR+ +  NKQ+P+VYMG NS+SPDPSSLCLEGTLD   VAGKIV
Sbjct: 361 VKLGNGRTVTGVSLYKGRMMLSTNKQYPVVYMGDNSTSPDPSSLCLEGTLDRRVVAGKIV 420

Query: 455 ICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAIGEREGKAIKQYA 514
           ICDRGISPRVQKG VVK+AGG+GMIL+NTAANGEELVADCHLVPAVA+GE E KAIK YA
Sbjct: 421 ICDRGISPRVQKGQVVKDAGGVGMILANTAANGEELVADCHLVPAVAVGETEAKAIKHYA 480

Query: 515 LTNRRATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKT 574
           LT+ RATATL FLGTR GV+PSPVVAAFSSRGPNF++LEILKPD+VAPGVNILAAWTG  
Sbjct: 481 LTSPRATATLAFLGTRTGVRPSPVVAAFSSRGPNFVSLEILKPDVVAPGVNILAAWTGAL 540

Query: 575 GPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNT 634
           GPSSL TD RRVKFNILSGTSMSCPHVSG+AAL+K++HP+WSP+AIKSALMTTAYVHDNT
Sbjct: 541 GPSSLPTDHRRVKFNILSGTSMSCPHVSGIAALLKARHPEWSPAAIKSALMTTAYVHDNT 600

Query: 635 YKPLKDSSAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLSPTQLKVFS 694
           +KPL+D+SAA  S+PYDHGAGHINPRKALDPGLVY+I+ QDY +FLCTQ L+P QLKVF+
Sbjct: 601 HKPLQDASAAEASTPYDHGAGHINPRKALDPGLVYDIEAQDYLEFLCTQRLTPMQLKVFT 660

Query: 695 KYSNRTCRGLLPNPGDLNYPAISAVFPEKTTVTSLTLHRTVTNVGPATSSYHAVVSPFKG 754
           KYSNR+C+  L +PGDLNYPAIS VFPE+T V+ LTLHRTVTNVGP  S+YHA+VSPFKG
Sbjct: 661 KYSNRSCKHALASPGDLNYPAISVVFPERTNVSLLTLHRTVTNVGPPVSNYHAIVSPFKG 720

Query: 755 ATVKVEPESLNFTRRYEKVSYRITFVTKKRQSMPEFGGLIWKDGSHKVRSPIVITWL 805
           A VKVEP +L FTR  +K+SY+ITF TK RQ+ PEFGGL+WKDG H+VRSPIV+ WL
Sbjct: 721 AYVKVEPRTLKFTRANQKLSYKITFTTKSRQATPEFGGLVWKDGVHRVRSPIVVVWL 777