Csa2G005300.1 (mRNA) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa2G005300.1
Type	mRNA
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Metal ion binding protein; contains IPR006121 (Heavy metal-associated domain, HMA)
Location	Chr2 : 858916 .. 859875 (+)
Sequence length	183

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GGTGGTGGTGAGGGTGTCAATGCATTGCAATGGCTGTGCTAGAAGAGTTGAGAAACATATATCCAAGATACAAGGTATTTTTTTTTAATAGTAATTATTATTATTACAAGGGAAATTGTAAAAAATGACAAAATGTAAATAGTTTGTATTTTTTAAAATTTATTAAAAAATGCAAAACGTTGATGTAAATTTTATGAATAGTAATTAAGAATGTAATAACATTTTACAAAAGTTGTAAATATAACAAAATTTATCGATGATAGATTCTGATAGACCAATATTTACCACATAGTCGACTTTGACCAATTTTGTTATATTTGTAATTTTTTAAAAATATTTTACATATTTAATTATTTTGAATCTAATAGCTATATTTTGTAATAAAACGATTTTTTTTAAACTGTGTCCAGTACAATTTGATGGTTTTGAAATTTGGTTCAAATCCCCACATATATTGTTTCTACATCCCAATTTCGTTAATAAAACAGTCCATGAACTAAAAGATTTGTACAGCACCCATCAAACATGCTTAATCATCTAATAATGTTTTACGTAAATAACTATGTATATTTAATATCGTATTTACTAATAAAGTATGGAGATTGTATTAGATGGCAGAAAAACAAAAGTCACAAAAATAAAAAATCAAACTTATAGTACTTTTTTGTATATTACAAATTTGACATATTTTTAAATTTGTCATTTAAAAAATGTAGATGATGTGGACCATGTTATCATATTTTTTTTTGTTATTTTTGTAAACTCGCCAATAAAGTAGTTATCAAAATAAAATTTAAATCTAGGTTGTTTGTCTTATAATACTTGCTTTTAGGCGTGGAATCATGGAAGGTAGATATGGAAAGAGAGACTGTGGTTGTTACGGGAGATGTTTTTCCTTTTGAAGTTATGCAATGTATTTCTAAAGTGAAGAGTGTAGAGATTTTGGAGCCTCAGGTTTGA

mRNA sequence

ATGCATTGCAATGGCTGTGCTAGAAGAGTTGAGAAACATATATCCAAGATACAAGGCGTGGAATCATGGAAGGTAGATATGGAAAGAGAGACTGTGGTTGTTACGGGAGATGTTTTTCCTTTTGAAGTTATGCAATGTATTTCTAAAGTGAAGAGTGTAGAGATTTTGGAGCCTCAGGTTTGA

Coding sequence (CDS)

ATGCATTGCAATGGCTGTGCTAGAAGAGTTGAGAAACATATATCCAAGATACAAGGCGTGGAATCATGGAAGGTAGATATGGAAAGAGAGACTGTGGTTGTTACGGGAGATGTTTTTCCTTTTGAAGTTATGCAATGTATTTCTAAAGTGAAGAGTGTAGAGATTTTGGAGCCTCAGGTTTGA

Protein sequence

MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEILEPQV*

Homology

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BLAST of Csa2G005300.1 vs. TrEMBL
Match: A0A0A0LFW6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G005300 PE=4 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 1.6e-26
Identity = 60/60 (100.00%), Postives = 60/60 (100.00%), Query Frame = 1


Query: 1  MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEILEPQV 60
          MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEILEPQV
Sbjct: 1  MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEILEPQV 60

[Close]

BLAST of Csa2G005300.1 vs. TrEMBL
Match: A0A022QIY3_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a015876mg PE=4 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 5.4e-14
Identity = 34/59 (57.63%), Postives = 51/59 (86.44%), Query Frame = 1


Query: 1   MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEILEPQ 60
           MHCNGCAR++EKHISK++GV S+KVD+E + VVVTGD+ PF+V++ +SK+K+ ++ + Q
Sbjct: 83  MHCNGCARKIEKHISKLEGVISYKVDLETKMVVVTGDIAPFQVLESVSKIKNAQLWDDQ 141

[Close]

BLAST of Csa2G005300.1 vs. TrEMBL
Match: M0TTR4_MUSAM (Uncharacterized protein OS=Musa acuminata subsp. malaccensis PE=4 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 7.1e-14
Identity = 35/55 (63.64%), Postives = 48/55 (87.27%), Query Frame = 1


Query: 1   MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEI 56
           MHCNGCAR++EKHISK++GV S+KVD+E++ VVV GD+ PFEV++ +SKVK  E+
Sbjct: 100 MHCNGCARKIEKHISKMEGVSSFKVDLEKKKVVVVGDITPFEVLESVSKVKFAEL 154

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BLAST of Csa2G005300.1 vs. TrEMBL
Match: M5VRG3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013199mg PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 9.3e-14
Identity = 38/55 (69.09%), Postives = 47/55 (85.45%), Query Frame = 1


Query: 1   MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEI 56
           MHCNGCAR+VEKHISKI+GV S+KVD+E + VVV GDV P EV++ +SKVK+ EI
Sbjct: 76  MHCNGCARKVEKHISKIEGVTSYKVDLESKMVVVIGDVLPREVLESVSKVKNTEI 130

[Close]

BLAST of Csa2G005300.1 vs. TrEMBL
Match: G7KNF6_MEDTR (Heavy metal transport/detoxification superfamily protein OS=Medicago truncatula GN=MTR_6g086020 PE=2 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 1.2e-13
Identity = 35/55 (63.64%), Postives = 48/55 (87.27%), Query Frame = 1


Query: 1   MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEI 56
           MHC+GCAR+VEKHISK++GV S+KVD++ + VVV GD+ PFEV++ +SKVK+ EI
Sbjct: 73  MHCHGCARKVEKHISKLEGVSSYKVDLDTKMVVVMGDILPFEVLESVSKVKNAEI 127

[Close]

BLAST of Csa2G005300.1 vs. TAIR10
Match: AT3G24450.1 (AT3G24450.1 Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 67.4 bits (163), Expect = 3.5e-12
Identity = 28/55 (50.91%), Postives = 41/55 (74.55%), Query Frame = 1


Query: 1   MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEI 56
           MHC GCA++VEKHISK+ GV  +KV++E + VVV G++ P +V++ I KVK+ ++
Sbjct: 82  MHCYGCAKKVEKHISKLDGVTWYKVELESKKVVVKGNILPVDVLESICKVKNAQL 136

[Close]

BLAST of Csa2G005300.1 vs. TAIR10
Match: AT5G02600.2 (AT5G02600.2 Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 63.9 bits (154), Expect = 3.9e-11
Identity = 27/54 (50.00%), Postives = 37/54 (68.52%), Query Frame = 1


Query: 1   MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVE 55
           +HC GCA +V+KH+SK++GV S+ +D   + V VTGDV P  V+  ISKVK+ +
Sbjct: 258 LHCKGCAGKVKKHLSKLKGVTSYNIDFAAKKVTVTGDVTPLTVLASISKVKNAQ 311

[Close]

BLAST of Csa2G005300.1 vs. TAIR10
Match: AT3G53530.2 (AT3G53530.2 Chloroplast-targeted copper chaperone protein)

HSP 1 Score: 63.5 bits (153), Expect = 5.0e-11
Identity = 28/58 (48.28%), Postives = 38/58 (65.52%), Query Frame = 1


Query: 2   HCNGCARRVEKHISKIQ-GVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEILEP 59
           HC GC  +V+KH+SK+Q GV S+ +D   + V VTGD+ P EV+ C+SKVK+ +   P
Sbjct: 179 HCRGCQGKVKKHLSKMQVGVTSFNIDFASKKVTVTGDITPLEVLGCLSKVKNAQFWTP 236

[Close]

BLAST of Csa2G005300.1 vs. TAIR10
Match: AT2G37390.1 (AT2G37390.1 Chloroplast-targeted copper chaperone protein)

HSP 1 Score: 62.0 bits (149), Expect = 1.5e-10
Identity = 24/54 (44.44%), Postives = 37/54 (68.52%), Query Frame = 1


Query: 1   MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVE 55
           +HC GC  +V KH++++QGV S+ +D   + V VTGD+ P E++  ISKVK+ +
Sbjct: 189 LHCRGCEGKVRKHLARMQGVTSFNIDFAAKKVTVTGDITPLEILDSISKVKNAQ 242

[Close]

BLAST of Csa2G005300.1 vs. TAIR10
Match: AT2G28660.1 (AT2G28660.1 Chloroplast-targeted copper chaperone protein)

HSP 1 Score: 55.8 bits (133), Expect = 1.1e-08
Identity = 23/55 (41.82%), Postives = 36/55 (65.45%), Query Frame = 1


Query: 1   MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEI 56
           +HC GC  +V KHISK++GV S+ +D+  + V V G + P  +++ ISKVK  ++
Sbjct: 191 IHCKGCEGKVRKHISKMEGVTSYTIDLATKKVTVVGKITPVGLVESISKVKFAQL 245

[Close]

BLAST of Csa2G005300.1 vs. NCBI nr
Match: gi|700205514|gb|KGN60633.1| (hypothetical protein Csa_2G005300 [Cucumis sativus])

HSP 1 Score: 125.9 bits (315), Expect = 2.3e-26
Identity = 60/60 (100.00%), Postives = 60/60 (100.00%), Query Frame = 1


Query: 1  MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEILEPQV 60
          MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEILEPQV
Sbjct: 1  MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEILEPQV 60

[Close]

BLAST of Csa2G005300.1 vs. NCBI nr
Match: gi|659071114|ref|XP_008458177.1| (PREDICTED: uncharacterized protein LOC103497691 [Cucumis melo])

HSP 1 Score: 113.6 bits (283), Expect = 1.2e-22
Identity = 55/59 (93.22%), Postives = 56/59 (94.92%), Query Frame = 1


Query: 1   MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEILEPQ 60
           MHCNGCARRVEK ISKIQGVESWKVDMERE VVVTGDVFPFEVM+CISKVKSVEILE Q
Sbjct: 87  MHCNGCARRVEKLISKIQGVESWKVDMERERVVVTGDVFPFEVMECISKVKSVEILESQ 145

[Close]

BLAST of Csa2G005300.1 vs. NCBI nr
Match: gi|719980545|ref|XP_010249835.1| (PREDICTED: copper transport protein ATX1 [Nelumbo nucifera])

HSP 1 Score: 86.3 bits (212), Expect = 2.1e-14
Identity = 38/57 (66.67%), Postives = 49/57 (85.96%), Query Frame = 1


Query: 1   MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEILE 58
           MHCNGCAR+VEKHISK++GV S+KVD+E + VVV GDV PFEV++ +SKVK+ E+ E
Sbjct: 79  MHCNGCARKVEKHISKMEGVTSYKVDLESKKVVVIGDVLPFEVLESVSKVKNAELWE 135

[Close]

BLAST of Csa2G005300.1 vs. NCBI nr
Match: gi|658004639|ref|XP_008337448.1| (PREDICTED: uncharacterized protein LOC103400574 [Malus domestica])

HSP 1 Score: 86.3 bits (212), Expect = 2.1e-14
Identity = 39/55 (70.91%), Postives = 48/55 (87.27%), Query Frame = 1


Query: 1   MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEI 56
           MHCNGCA++VEKHISKI+GV S+KVD+E + VVV GDV PFEV++ ISKVK+ EI
Sbjct: 76  MHCNGCAKKVEKHISKIEGVTSYKVDLESKMVVVIGDVLPFEVLESISKVKNAEI 130

[Close]

BLAST of Csa2G005300.1 vs. NCBI nr
Match: gi|470101233|ref|XP_004287081.1| (PREDICTED: uncharacterized protein LOC101298054 [Fragaria vesca subsp. vesca])

HSP 1 Score: 85.5 bits (210), Expect = 3.5e-14
Identity = 38/55 (69.09%), Postives = 48/55 (87.27%), Query Frame = 1


Query: 1   MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEI 56
           MHCNGCAR+VEKHISK++GV S+KVD+E + VVV GDV P+EV+Q +SKVK+ EI
Sbjct: 76  MHCNGCARKVEKHISKMEGVTSYKVDLESKMVVVIGDVLPYEVVQSVSKVKNAEI 130

The following BLAST results are available for this feature:

BLAST of Csa2G005300.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LFW6_CUCSA	1.6e-26	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G005300 PE=4 SV=1	[more]
A0A022QIY3_ERYGU	5.4e-14	57.63	Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a015876mg PE=4 SV=1	[more]
M0TTR4_MUSAM	7.1e-14	63.64	Uncharacterized protein OS=Musa acuminata subsp. malaccensis PE=4 SV=1	[more]
M5VRG3_PRUPE	9.3e-14	69.09	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013199mg PE=4 SV=1	[more]
G7KNF6_MEDTR	1.2e-13	63.64	Heavy metal transport/detoxification superfamily protein OS=Medicago truncatula ...	[more]

BLAST of Csa2G005300.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G24450.1	3.5e-12	50.91	Heavy metal transport/detoxification superfamily protein	[more]
AT5G02600.2	3.9e-11	50.00	Heavy metal transport/detoxification superfamily protein	[more]
AT3G53530.2	5.0e-11	48.28	Chloroplast-targeted copper chaperone protein	[more]
AT2G37390.1	1.5e-10	44.44	Chloroplast-targeted copper chaperone protein	[more]
AT2G28660.1	1.1e-08	41.82	Chloroplast-targeted copper chaperone protein	[more]

BLAST of Csa2G005300.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700205514\|gb\|KGN60633.1\|	2.3e-26	100.00	hypothetical protein Csa_2G005300 [Cucumis sativus]	[more]
gi\|659071114\|ref\|XP_008458177.1\|	1.2e-22	93.22	PREDICTED: uncharacterized protein LOC103497691 [Cucumis melo]	[more]
gi\|719980545\|ref\|XP_010249835.1\|	2.1e-14	66.67	PREDICTED: copper transport protein ATX1 [Nelumbo nucifera]	[more]
gi\|658004639\|ref\|XP_008337448.1\|	2.1e-14	70.91	PREDICTED: uncharacterized protein LOC103400574 [Malus domestica]	[more]
gi\|470101233\|ref\|XP_004287081.1\|	3.5e-14	69.09	PREDICTED: uncharacterized protein LOC101298054 [Fragaria vesca subsp. vesca]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR006121	HMA_dom

Vocabulary: **Biological Process**
Term	Definition
GO:0030001	metal ion transport

Vocabulary: **Molecular Function**
Term	Definition
GO:0046872	metal ion binding

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030001	metal ion transport
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0046872	metal ion binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Csa2G005300	Csa2G005300	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Csa2G005300.1	Csa2G005300.1-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csa2G005300.1.utr5p1	Csa2G005300.1.utr5p1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csa2G005300.1.cds1	Csa2G005300.1.cds1	CDS
Csa2G005300.1.cds2	Csa2G005300.1.cds2	CDS

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006121	Heavy metal-associated domain, HMA	PFAM	PF00403	HMA	coord: 1..40 score: 4.1
IPR006121	Heavy metal-associated domain, HMA	PROFILE	PS50846	HMA_2	coord: 1..60 score: 12
IPR006121	Heavy metal-associated domain, HMA	unknown	SSF55008	HMA, heavy metal-associated domain	coord: 1..50 score: 1.1
None	No IPR available	GENE3D	G3DSA:3.30.70.100		coord: 1..50 score: 2.5
None	No IPR available	PANTHER	PTHR22814	COPPER TRANSPORT PROTEIN ATOX1-RELATED	coord: 1..51 score: 4.6
None	No IPR available	PANTHER	PTHR22814:SF104	SUBFAMILY NOT NAMED	coord: 1..51 score: 4.6