Csa1G073100.1 (mRNA) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa1G073100.1
Type	mRNA
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Polyphenol oxidase; contains IPR008922 (Uncharacterised domain, di-copper centre)
Location	Chr1 : 7422284 .. 7423510 (-)
Sequence length	915

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTACTGTTTGGACTTGGAGGGCTTCACGGCGTCACGACTCTTAGCAGCGATAACTTTATTTGCATTGGCGGATTCGCTGTTGTCGCCGGATGTTCTTAACTGCGTGACAAAAGGAAAACACCAGCAGGGGACGATGGATGTTGCCCACCTGAAATCGAAGAAATTATTGAGTTTGTCCCCCCGGTCATTCCAACACCCCGTATCCGACCCGCTGCCCATACAATGACCACAACCCAGCTTGATGATTTGAAAAAAGGGATTGAAAAGATGAGAGCCCTTAGTGACGATGACCCTCGTAGTTTGTATCAACAAGCTAATGTCCACTGTGCCCATTGCAATGATGCATCCTTCTGAATCAGTAGCTAAAGTTCAAGTTCATAGCAACGCTTTATTCTTCCCACCTCACCGTGCTTATCTTTACTTCTTTGAGAGATTTTTAAGTTCTTTGATTGGGAACCCAGATTTTGCTATACCTTTTTGGAATTGGGATACACCTGATGGCATGTACAATATGACTGAACCCTACAACGACCCGAATTCATCGCTTTACGATAAGTTCCGCGATCCACTTCTCATGTTAAAAAAAAATTGCTTTATCTTAACTACGACAGAAAAACCGAAGACCATGGTTTCGACATTGTTGAATGTAAACTCCATTGGATGAAACAACGAATGAATGTTACGAGCGCCCGAAGGTTCCTTGGAAATATAGTTGAAGTTGGGAAGGTCCCCACCCACAATGACATGGGTAGCACATTGAACAATCTCCACATAATAACGTTCACAATTGGGTTGGCAATAAAAACATACGTGGTCGTATAGACATGGGAGATTTTTCGACCGCAGGACGAGATCCATTGTTTTACGCTCATCACGTGAATGTCGATGGCATGTGGAAGATTTGGAAAGAAATGGGGAATCAAGATCACACCGACGAGGGTTGGGTAAACTCGACATTTGTGTTTTACGATGAGAATCAAAACGCTGTACGTATCAAAGTTTCGGATTGTTTGGACAGTAAGAAACTTGGGTATGATTATGAAAAGGTACCCATTTCATGGAAGCATTTGAAGAAAACCCCGTTACCAAATAATAATTGTATAATTAATAGAGAATTATTCCAGCTGCCTCCAATGAAGCAAAGTTTCCTGTGAAGATGAAACCTAAGCTTGGTGGAGAAAGCGTCAGTATTGGAGGGATAACGGCTCAGCTTCAGCCTGTGTAA

mRNA sequence

ATGTACTGTTTGGACTTGGAGGGCTTCACGGCGTCACGACTCTTAGCAGCGATAACTTTATTTGCATTGGCGGATTCGCTGTTGTCGCCGGATGTTCTTAACTGCGTGACAAAAGGAAAACACCAGCAGGGGACGATGGATGTTGCCCACCTGAAATCGAAGAAATTATTGAGTTTGTCCCCCCGGTCATTCCAACACCCCCTAATGTCCACTGTGCCCATTGCAATGATGCATCCTTCTGAATCAGTAGCTAAAGTTCAAGTTCATAGCAACGCTTTATTCTTCCCACCTCACCGTGCTTATCTTTACTTCTTTGAGAGATTTTTAAGTTCTTTGATTGGGAACCCAGATTTTGCTATACCTTTTTGGAATTGGGATACACCTGATGGCATGTACAATATGACTGAACCCTACAACGACCCGAATTCATCGCTTTACGATAAGTTCCGCGATCCACTTCTCATAAAAACCGAAGACCATGGTTTCGACATTGTTGAATGTAAACTCCATTGGATGAAACAACGAATGAATGTTACGAGCGCCCGAAGGTTCCTTGGAAATATAGTTGAAGTTGGGAAGGTCCCCACCCACAATGACATGGGACGAGATCCATTGTTTTACGCTCATCACGTGAATGTCGATGGCATGTGGAAGATTTGGAAAGAAATGGGGAATCAAGATCACACCGACGAGGGTTGGGTAAACTCGACATTTGTGTTTTACGATGAGAATCAAAACGCTGTACGTATCAAAGTTTCGGATTGTTTGGACAGTAAGAAACTTGGGTATGATTATGAAAAGAGAATTATTCCAGCTGCCTCCAATGAAGCAAAGTTTCCTGTGAAGATGAAACCTAAGCTTGGTGGAGAAAGCGTCAGTATTGGAGGGATAACGGCTCAGCTTCAGCCTGTGTAA

Coding sequence (CDS)

ATGTACTGTTTGGACTTGGAGGGCTTCACGGCGTCACGACTCTTAGCAGCGATAACTTTATTTGCATTGGCGGATTCGCTGTTGTCGCCGGATGTTCTTAACTGCGTGACAAAAGGAAAACACCAGCAGGGGACGATGGATGTTGCCCACCTGAAATCGAAGAAATTATTGAGTTTGTCCCCCCGGTCATTCCAACACCCCCTAATGTCCACTGTGCCCATTGCAATGATGCATCCTTCTGAATCAGTAGCTAAAGTTCAAGTTCATAGCAACGCTTTATTCTTCCCACCTCACCGTGCTTATCTTTACTTCTTTGAGAGATTTTTAAGTTCTTTGATTGGGAACCCAGATTTTGCTATACCTTTTTGGAATTGGGATACACCTGATGGCATGTACAATATGACTGAACCCTACAACGACCCGAATTCATCGCTTTACGATAAGTTCCGCGATCCACTTCTCATAAAAACCGAAGACCATGGTTTCGACATTGTTGAATGTAAACTCCATTGGATGAAACAACGAATGAATGTTACGAGCGCCCGAAGGTTCCTTGGAAATATAGTTGAAGTTGGGAAGGTCCCCACCCACAATGACATGGGACGAGATCCATTGTTTTACGCTCATCACGTGAATGTCGATGGCATGTGGAAGATTTGGAAAGAAATGGGGAATCAAGATCACACCGACGAGGGTTGGGTAAACTCGACATTTGTGTTTTACGATGAGAATCAAAACGCTGTACGTATCAAAGTTTCGGATTGTTTGGACAGTAAGAAACTTGGGTATGATTATGAAAAGAGAATTATTCCAGCTGCCTCCAATGAAGCAAAGTTTCCTGTGAAGATGAAACCTAAGCTTGGTGGAGAAAGCGTCAGTATTGGAGGGATAACGGCTCAGCTTCAGCCTGTGTAA

Protein sequence

MYCLDLEGFTASRLLAAITLFALADSLLSPDVLNCVTKGKHQQGTMDVAHLKSKKLLSLSPRSFQHPLMSTVPIAMMHPSESVAKVQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAIPFWNWDTPDGMYNMTEPYNDPNSSLYDKFRDPLLIKTEDHGFDIVECKLHWMKQRMNVTSARRFLGNIVEVGKVPTHNDMGRDPLFYAHHVNVDGMWKIWKEMGNQDHTDEGWVNSTFVFYDENQNAVRIKVSDCLDSKKLGYDYEKRIIPAASNEAKFPVKMKPKLGGESVSIGGITAQLQPV*

Homology

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BLAST of Csa1G073100.1 vs. Swiss-Prot
Match: PPO_VICFA (Polyphenol oxidase A1, chloroplastic OS=Vicia faba PE=1 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 9.2e-39
Identity = 105/250 (42.00%), Postives = 134/250 (53.60%), Query Frame = 1


Query: 85  KVQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAIPFWNWDTPDGMYNMTEPYNDPNSS 144
           K+QVH + LFFP HR YLYF+ER L SLI +P FA+PFWN+D PDGM  +   Y D  S 
Sbjct: 198 KLQVHGSWLFFPFHRWYLYFYERILGSLINDPTFALPFWNYDAPDGM-QLPTIYADKASP 257

Query: 145 LYDKFRD-----PLLIKTE--DHGFDI-----VECKLHWMKQRM--NVTSARRFLGN--- 204
           LYD+ R+     P LI     D G DI     ++  L  M +++  N  ++R FLGN   
Sbjct: 258 LYDELRNASHQPPTLIDLNFCDIGSDIDRNELIKTNLSIMYRQVYSNGKTSRLFLGNPYR 317

Query: 205 -----------IVEVGKVPTHN-----------DMG------RDPLFYAHHVNVDGMWKI 264
                      I  V   P H            DMG      RDP+FY+HH NVD +W I
Sbjct: 318 AGDAEPQGAGSIENVPHAPVHTWTGDNTQTNIEDMGIFYSAARDPIFYSHHSNVDRLWYI 377

Query: 265 WKEMGNQDH--TDEGWVNSTFVFYDENQNAVRIKVSDCLDSKKLGYDYEKRIIPAASNEA 288
           WK +G + H  TD+ W+ S F+FYDEN+N VR+ V D LD  KLGY Y+   +P    +A
Sbjct: 378 WKTLGGKKHDFTDKDWLESGFLFYDENKNLVRVNVKDSLDIDKLGYAYQD--VPIPWEKA 437

[Close]

BLAST of Csa1G073100.1 vs. Swiss-Prot
Match: PPO1_IPOBA (Polyphenol oxidase I, chloroplastic OS=Ipomoea batatas GN=co-1 PE=1 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 2.7e-38
Identity = 101/269 (37.55%), Postives = 138/269 (51.30%), Query Frame = 1


Query: 85  KVQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAIPFWNWDTPDGMYNMTEPYNDPNSS 144
           ++QVH++ LFFP HR YLYF+ER L  LIG+P F +PFWNWD P GM  + +  ND  SS
Sbjct: 105 EIQVHNSWLFFPFHRWYLYFYERILGKLIGDPSFGLPFWNWDNPGGMV-LPDFLNDSTSS 164

Query: 145 LYDKFRD-----PLLIKTEDHGFDI-------VECKLHWMKQRM--NVTSARRFL----- 204
           LYD  R+     P+++    +G D        +   L  M ++M  N  +A  FL     
Sbjct: 165 LYDSNRNQSHLPPVVVDLGYNGADTDVTDQQRITDNLALMYKQMVTNAGTAELFLGKAYR 224

Query: 205 ---------GNIVEVGKVPTHN-----------DMG------RDPLFYAHHVNVDGMWKI 264
                    G+I     +P H            DMG      RD  FY HH NVD MW I
Sbjct: 225 AGDAPSPGAGSIETSPHIPIHRWVGDPRNTNNEDMGNFYSAGRDIAFYCHHSNVDRMWTI 284

Query: 265 WKEMGNQ----DHTDEGWVNSTFVFYDENQNAVRIKVSDCLDSKKLGYDYEKRIIPAASN 305
           W+++  +    D+TD  W+N+TF+FYDEN  AV++++ D LD++K+GY Y K  +P   +
Sbjct: 285 WQQLAGKPRKRDYTDSDWLNATFLFYDENGQAVKVRIGDSLDNQKMGYKYAKTPLPWLDS 344

[Close]

BLAST of Csa1G073100.1 vs. Swiss-Prot
Match: PPOF_SOLLC (Polyphenol oxidase F, chloroplastic OS=Solanum lycopersicum PE=3 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 1.6e-35
Identity = 91/227 (40.09%), Postives = 117/227 (51.54%), Query Frame = 1


Query: 85  KVQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAIPFWNWDTPDGMYNMTEPYNDPNSS 144
           ++QVH + LFFP HR YLYF+ER L SLI +P FA+P+WNWD P GM  +   ++   SS
Sbjct: 193 ELQVHFSWLFFPFHRWYLYFYERILGSLINDPTFALPYWNWDHPKGM-RIPPMFDREGSS 252

Query: 145 LYDK-----FRDPLLIKTEDHGFDIVECKLHWMKQ------RMNVTSA------------ 204
           LYD+      R+  +I     G D+   +L  M        R  VT+A            
Sbjct: 253 LYDEKRNQNHRNGTIIDLGHFGKDVRTPQLQIMTNNLTLMYRQMVTNAPCPSQFFGAPLG 312

Query: 205 ---RRFLGNIVEVGKVPTH------------NDMGR------DPLFYAHHVNVDGMWKIW 264
                 +G I  +   P H             DMG       DPLFY HH NVD MW  W
Sbjct: 313 SDPEPGMGTIENIPHTPVHIWTGDSPRQGHGEDMGNFYSAGLDPLFYCHHANVDRMWNEW 372

Query: 265 KEMG--NQDHTDEGWVNSTFVFYDENQNAVRIKVSDCLDSKKLGYDY 266
           K +G   +D +++ W+NS F FYDEN+N  R+KV DCLDSKK+G+DY
Sbjct: 373 KLIGGKRRDLSNKDWLNSEFFFYDENRNPYRVKVRDCLDSKKMGFDY 418

[Close]

BLAST of Csa1G073100.1 vs. Swiss-Prot
Match: PPOE_SOLLC (Polyphenol oxidase E, chloroplastic OS=Solanum lycopersicum PE=3 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 2.1e-35
Identity = 91/229 (39.74%), Postives = 118/229 (51.53%), Query Frame = 1


Query: 85  KVQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAIPFWNWDTPDGMYNMTEPYNDPNSS 144
           ++QVH + LFFP HR YLYF+ER L SLI +P FA+P+WNWD P GM  +   ++   SS
Sbjct: 193 ELQVHFSWLFFPFHRWYLYFYERILGSLINDPTFALPYWNWDHPKGM-RIPPMFDREGSS 252

Query: 145 LYDK-----FRDPLLIKTEDHGFDIVECKLHWMKQ------RMNVTSA---RRFLGNIVE 204
           LYD+      R+  +I     G ++   +L  M        R  VT+A    +F G    
Sbjct: 253 LYDEKRNQNHRNGTIIDLGHFGKEVDTPQLQIMTNNLTLMYRQMVTNAPCPSQFFGAAYP 312

Query: 205 VGKVPTHN--------------------------DMGR------DPLFYAHHVNVDGMWK 264
           +G  P+                            DMG       DP+FY HH NVD MW 
Sbjct: 313 LGSEPSPGQGTIENIPHTPVHIWTGDKPRQKNGEDMGNFYSAGLDPIFYCHHANVDRMWN 372

Query: 265 IWKEMGNQ--DHTDEGWVNSTFVFYDENQNAVRIKVSDCLDSKKLGYDY 266
            WK +G +  D TD+ W+NS F FYDEN+N  R+KV DCLDSKK+G+DY
Sbjct: 373 EWKLIGGKRRDLTDKDWLNSEFFFYDENRNPYRVKVRDCLDSKKMGFDY 420

[Close]

BLAST of Csa1G073100.1 vs. Swiss-Prot
Match: PPOB_SOLLC (Polyphenol oxidase B, chloroplastic OS=Solanum lycopersicum PE=3 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 5.2e-34
Identity = 92/263 (34.98%), Postives = 126/263 (47.91%), Query Frame = 1


Query: 85  KVQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAIPFWNWDTPDGMYNMTEPYNDPNSS 144
           ++QVH++ LFFP HR YLYF+ER L  LI +P FA+P+WNWD P GM  +   ++   SS
Sbjct: 195 ELQVHNSWLFFPFHRWYLYFYERILGKLIDDPTFALPYWNWDHPKGM-RLPPMFDREGSS 254

Query: 145 LYD-----KFRDPLLIKTEDHGFDIVECKLHWMKQRMNVT---------------SARRF 204
           LYD     + R+  ++     G  +   +L  M   + +                 A   
Sbjct: 255 LYDERRNQQVRNGTVLDLGSFGDKVETTQLQLMSNNLTLMYRQMVTNAPCPLLFFGAPYV 314

Query: 205 LGNIVE-------VGKVPTH-------------------NDMGR------DPLFYAHHVN 264
           LGN VE       +  +P H                    DMG       DP+FY HH N
Sbjct: 315 LGNNVEAPGTIETIPHIPVHIWAGTVRGSKFPNGDVSYGEDMGNFYSAGLDPVFYCHHGN 374

Query: 265 VDGMWKIWKEMG--NQDHTDEGWVNSTFVFYDENQNAVRIKVSDCLDSKKLGYDYEKRII 294
           VD MW  WK +G   +D +++ W+NS F FYDE++N  R+KV DCLD+KK+GYDY     
Sbjct: 375 VDRMWNEWKAIGGKRRDISEKDWLNSEFFFYDEHKNPYRVKVRDCLDTKKMGYDY----A 434

[Close]

BLAST of Csa1G073100.1 vs. TrEMBL
Match: A0A0A0LRV2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G073100 PE=4 SV=1)

HSP 1 Score: 634.4 bits (1635), Expect = 7.1e-179
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 1


Query: 1   MYCLDLEGFTASRLLAAITLFALADSLLSPDVLNCVTKGKHQQGTMDVAHLKSKKLLSLS 60
           MYCLDLEGFTASRLLAAITLFALADSLLSPDVLNCVTKGKHQQGTMDVAHLKSKKLLSLS
Sbjct: 1   MYCLDLEGFTASRLLAAITLFALADSLLSPDVLNCVTKGKHQQGTMDVAHLKSKKLLSLS 60

Query: 61  PRSFQHPLMSTVPIAMMHPSESVAKVQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAI 120
           PRSFQHPLMSTVPIAMMHPSESVAKVQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAI
Sbjct: 61  PRSFQHPLMSTVPIAMMHPSESVAKVQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAI 120

Query: 121 PFWNWDTPDGMYNMTEPYNDPNSSLYDKFRDPLLIKTEDHGFDIVECKLHWMKQRMNVTS 180
           PFWNWDTPDGMYNMTEPYNDPNSSLYDKFRDPLLIKTEDHGFDIVECKLHWMKQRMNVTS
Sbjct: 121 PFWNWDTPDGMYNMTEPYNDPNSSLYDKFRDPLLIKTEDHGFDIVECKLHWMKQRMNVTS 180

Query: 181 ARRFLGNIVEVGKVPTHNDMGRDPLFYAHHVNVDGMWKIWKEMGNQDHTDEGWVNSTFVF 240
           ARRFLGNIVEVGKVPTHNDMGRDPLFYAHHVNVDGMWKIWKEMGNQDHTDEGWVNSTFVF
Sbjct: 181 ARRFLGNIVEVGKVPTHNDMGRDPLFYAHHVNVDGMWKIWKEMGNQDHTDEGWVNSTFVF 240

Query: 241 YDENQNAVRIKVSDCLDSKKLGYDYEKRIIPAASNEAKFPVKMKPKLGGESVSIGGITAQ 300
           YDENQNAVRIKVSDCLDSKKLGYDYEKRIIPAASNEAKFPVKMKPKLGGESVSIGGITAQ
Sbjct: 241 YDENQNAVRIKVSDCLDSKKLGYDYEKRIIPAASNEAKFPVKMKPKLGGESVSIGGITAQ 300

Query: 301 LQPV 305
           LQPV
Sbjct: 301 LQPV 304

[Close]

BLAST of Csa1G073100.1 vs. TrEMBL
Match: A0A0A0LUI1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G073090 PE=4 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 3.3e-59
Identity = 123/228 (53.95%), Postives = 144/228 (63.16%), Query Frame = 1


Query: 88  VHSNALFFPPHRAYLYFFERFLSSLIGNPDFAIPFWNWDTPDGMYNMTEPYNDPNSSLYD 147
           VHSNALFFP HRAYLYFFER L   IGNP+FAIPFWNWD+P GMY M E YN P+SSLYD
Sbjct: 179 VHSNALFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMY-MPEFYNRPSSSLYD 238

Query: 148 KFRDPL-----LI------KTEDHGFDIVECKLHWMKQRMNVTSARRFLGNIVEVGKVPT 207
             RDP      LI      KTEDH FD+V+C L WM  RM V S R FLG +++ G+ P 
Sbjct: 239 HLRDPRHDPHKLIYLNYNGKTEDHSFDVVDCNLRWMNDRMQVQSPRSFLGRVLKAGETPN 298

Query: 208 HNDMGR-------------------------------DPLFYAHHVNVDGMWKIWKEM-G 267
             DMG                                DPLFYAHH N+D +W IWK + G
Sbjct: 299 PTDMGTIEQSPHNNIHDWLGTKTPHFEDMGSFYSAALDPLFYAHHANIDRLWNIWKTLDG 358

Query: 268 N-QDHTDEGWVNSTFVFYDENQNAVRIKVSDCLDSKKLGYDYEKRIIP 272
           N +DH D  W+NS+FVFYDEN+NAV IKVSDCLD++KLGY Y++  +P
Sbjct: 359 NPKDHIDPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLGYVYQEVPLP 405

[Close]

BLAST of Csa1G073100.1 vs. TrEMBL
Match: A0A0A0LUI1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G073090 PE=4 SV=1)

HSP 1 Score: 33.9 bits (76), Expect = 4.2e+02
Identity = 13/24 (54.17%), Postives = 20/24 (83.33%), Query Frame = 1


Query: 281 VKMKPKLGGESVSIGGITAQLQPV 305
           VK++P +GGE V++GGI  QL+P+
Sbjct: 534 VKIEPMVGGEGVTVGGIKIQLEPL 557

HSP 2 Score: 179.9 bits (455), Expect = 4.8e-42
Identity = 102/233 (43.78%), Postives = 133/233 (57.08%), Query Frame = 1


Query: 86  VQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAIPFWNWDTPDGMYNMTEPYNDPNSSL 145
           +QVH++ LFFP HR YLYFFE+ L  LI +P FAIPFWNWD+P GM  M   Y +PNS L
Sbjct: 202 LQVHNSWLFFPFHRYYLYFFEKILGKLIDDPTFAIPFWNWDSPAGM-QMPAIYANPNSPL 261

Query: 146 YDKFRDP-----LLIKTEDHGF-------DIVECKLHWMKQRM--NVTSARRFLGNIVEV 205
           YD+ R+P      L+  + +G        D +   L+ M ++M  N  +A+ FLGN    
Sbjct: 262 YDENRNPNHQPPTLLDLDFNGTEESSSTRDQISSNLNVMYRQMVSNGKTAKLFLGNAYRA 321

Query: 206 G-------------------------KVPTHNDMG------RDPLFYAHHVNVDGMWKIW 265
           G                         + P   DMG      RDP+FYAHH NVD MW +W
Sbjct: 322 GDQPDPGAGSLENIPHGPIHIWCGDNRQPNLEDMGNFYSAGRDPIFYAHHSNVDRMWSVW 381

Query: 266 KEMGNQ--DHTDEGWVNSTFVFYDENQNAVRIKVSDCLDSKKLGYDYEKRIIP 272
           K +G +  D TD  W++S F+FYDEN N VR+KV DCLD++K+GYDY+K  IP
Sbjct: 382 KTLGGKRTDFTDSDWLDSGFLFYDENANLVRVKVRDCLDTRKMGYDYQKVDIP 433

[Close]

BLAST of Csa1G073100.1 vs. TrEMBL
Match: M0TW39_MUSAM (Uncharacterized protein OS=Musa acuminata subsp. malaccensis PE=4 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 3.4e-40
Identity = 89/194 (45.88%), Postives = 121/194 (62.37%), Query Frame = 1


Query: 85  KVQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAIPFWNWDTPDGMYNMTEPYNDPNSS 144
           ++QVH++ LFFP HR YLYF ER L  LIG+  FA+PFWNWD P GM  +   Y DP+SS
Sbjct: 214 ELQVHNSWLFFPWHRFYLYFHERILGKLIGDDTFALPFWNWDAPGGM-KLPSIYADPSSS 273

Query: 145 LYDKFRD-----PLLIKTEDHGFDIVECKLHWMKQRMNVTSARRFLGNIVEVGKVPTHND 204
           LYDKFRD     P+L+  + +G D        + Q + +   R+ +GN            
Sbjct: 274 LYDKFRDAKHQPPVLVDLDYNGTDPSFTDAEQIDQNLKI-MYRQDMGNFYSA-------- 333

Query: 205 MGRDPLFYAHHVNVDGMWKIWKEMG--NQDHTDEGWVNSTFVFYDENQNAVRIKVSDCLD 264
            GRDP+F+AHH NVD MW +WK++G  +QD  D+ W+N+TF+FYDEN + VR+ + DCL 
Sbjct: 334 -GRDPIFFAHHSNVDRMWYLWKKLGGKHQDFNDKDWLNTTFLFYDENADLVRVTLKDCLQ 393

Query: 265 SKKLGYDYEKRIIP 272
            + L YDY+   IP
Sbjct: 394 PEWLRYDYQDVEIP 396

[Close]

BLAST of Csa1G073100.1 vs. TrEMBL
Match: A0A0J8EMT5_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_8g183910 PE=4 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 5.8e-40
Identity = 107/271 (39.48%), Postives = 138/271 (50.92%), Query Frame = 1


Query: 54  KKLLSLSPRSFQHPLMSTVPIAMMHPSESVA-----KVQVHSNALFFPPHRAYLYFFERF 113
           K L S  PRSF     + V  A  + S S        +QVH++ LF+P HR YLYFFER 
Sbjct: 99  KALPSTDPRSFTQ--QANVHCAYCNGSYSPVGFPNLDLQVHNSWLFYPFHRWYLYFFERI 158

Query: 114 LSSLIGNPDFAIPFWNWDTPDGMYNMTEPYNDPNSSLYDKFRDPL--LIKTEDHGFDIVE 173
           L +LI +P FAIPFWNWDTP GM  M   Y DPNS LYD  RD      K  D  +D+ +
Sbjct: 159 LGTLINDPTFAIPFWNWDTPAGMV-MPALYTDPNSPLYDSVRDRAHQPPKVLDLNYDLTD 218

Query: 174 CK-------------LHWMKQRMNVTSARRFLGNIVEVGKVP------------------ 233
                          L + +   N ++AR FLG     G  P                  
Sbjct: 219 ANDLSNQQMISANLTLMYRQMVTNSSTARLFLGQPYRAGTRPNPGGGSVENVPHGTVHVW 278

Query: 234 -------THNDMGR------DPLFYAHHVNVDGMWKIWKEMG--NQDHTDEGWVNSTFVF 272
                     DMG       DPLFYAHH N+D MW IWK +G   QD T+  W++++F+F
Sbjct: 279 TGDRNQPNFEDMGNFYSAARDPLFYAHHANIDRMWSIWKTLGGNRQDFTETDWLDASFIF 338

[Close]

BLAST of Csa1G073100.1 vs. NCBI nr
Match: gi|700209554|gb|KGN64650.1| (hypothetical protein Csa_1G073100 [Cucumis sativus])

HSP 1 Score: 634.4 bits (1635), Expect = 1.0e-178
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 1


Query: 1   MYCLDLEGFTASRLLAAITLFALADSLLSPDVLNCVTKGKHQQGTMDVAHLKSKKLLSLS 60
           MYCLDLEGFTASRLLAAITLFALADSLLSPDVLNCVTKGKHQQGTMDVAHLKSKKLLSLS
Sbjct: 1   MYCLDLEGFTASRLLAAITLFALADSLLSPDVLNCVTKGKHQQGTMDVAHLKSKKLLSLS 60

Query: 61  PRSFQHPLMSTVPIAMMHPSESVAKVQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAI 120
           PRSFQHPLMSTVPIAMMHPSESVAKVQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAI
Sbjct: 61  PRSFQHPLMSTVPIAMMHPSESVAKVQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAI 120

Query: 121 PFWNWDTPDGMYNMTEPYNDPNSSLYDKFRDPLLIKTEDHGFDIVECKLHWMKQRMNVTS 180
           PFWNWDTPDGMYNMTEPYNDPNSSLYDKFRDPLLIKTEDHGFDIVECKLHWMKQRMNVTS
Sbjct: 121 PFWNWDTPDGMYNMTEPYNDPNSSLYDKFRDPLLIKTEDHGFDIVECKLHWMKQRMNVTS 180

Query: 181 ARRFLGNIVEVGKVPTHNDMGRDPLFYAHHVNVDGMWKIWKEMGNQDHTDEGWVNSTFVF 240
           ARRFLGNIVEVGKVPTHNDMGRDPLFYAHHVNVDGMWKIWKEMGNQDHTDEGWVNSTFVF
Sbjct: 181 ARRFLGNIVEVGKVPTHNDMGRDPLFYAHHVNVDGMWKIWKEMGNQDHTDEGWVNSTFVF 240

Query: 241 YDENQNAVRIKVSDCLDSKKLGYDYEKRIIPAASNEAKFPVKMKPKLGGESVSIGGITAQ 300
           YDENQNAVRIKVSDCLDSKKLGYDYEKRIIPAASNEAKFPVKMKPKLGGESVSIGGITAQ
Sbjct: 241 YDENQNAVRIKVSDCLDSKKLGYDYEKRIIPAASNEAKFPVKMKPKLGGESVSIGGITAQ 300

Query: 301 LQPV 305
           LQPV
Sbjct: 301 LQPV 304

[Close]

BLAST of Csa1G073100.1 vs. NCBI nr
Match: gi|659068847|ref|XP_008446626.1| (PREDICTED: polyphenol oxidase I, chloroplastic-like [Cucumis melo])

HSP 1 Score: 241.1 bits (614), Expect = 2.5e-60
Identity = 124/231 (53.68%), Postives = 149/231 (64.50%), Query Frame = 1


Query: 85  KVQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAIPFWNWDTPDGMYNMTEPYNDPNSS 144
           + +VHSNALFFP HRAYLYFFER L   IGNPDFAIPFWNWD+P GM+ M E YN P+SS
Sbjct: 342 RFEVHSNALFFPFHRAYLYFFERILRHYIGNPDFAIPFWNWDSPPGMH-MPEFYNRPSSS 401

Query: 145 LYDKFRDPL-----LI------KTEDHGFDIVECKLHWMKQRMNVTSARRFLGNIVEVGK 204
           LYD  RDP      LI      KTEDH FD+V+C L WM  RM V S R FLG +++ G+
Sbjct: 402 LYDNLRDPRHDPHKLIDLNYNGKTEDHSFDVVDCNLRWMNDRMKVQSPRSFLGRVLKAGE 461

Query: 205 VPTHNDMGR-------------------------------DPLFYAHHVNVDGMWKIWKE 264
            P  +DMG                                DPLFYAHH N+D +W IWK 
Sbjct: 462 TPKPSDMGTIEQSPHNNIHDWVGTKTPHFEDMGSFYSAALDPLFYAHHTNIDRLWNIWKT 521

Query: 265 M-GN-QDHTDEGWVNSTFVFYDENQNAVRIKVSDCLDSKKLGYDYEKRIIP 272
           + GN +DHTD  W+NS+FVFYDEN+NAVRI+VSDCLD++KLGY Y++  +P
Sbjct: 522 LDGNPKDHTDPDWLNSSFVFYDENKNAVRIEVSDCLDTEKLGYVYQEVPLP 571

[Close]

BLAST of Csa1G073100.1 vs. NCBI nr
Match: gi|659068847|ref|XP_008446626.1| (PREDICTED: polyphenol oxidase I, chloroplastic-like [Cucumis melo])

HSP 1 Score: 118.2 bits (295), Expect = 2.4e-23
Identity = 69/149 (46.31%), Postives = 86/149 (57.72%), Query Frame = 1


Query: 61  PRSFQHPLMSTVPIAMMHPS----ESVAKVQVHSNALFFPPHRAYLYFFERFLSSLIGNP 120
           PRSF H   + V  A    +    +S    QVH NALFFP HRAYLYFFER L  L+  P
Sbjct: 7   PRSFAH--QAKVHCAYCDDAYKQMDSTMDFQVHENALFFPFHRAYLYFFERILCHLLEKP 66

Query: 121 DFAIPFWNWDTPDGMYNMTEPYNDPNSSLYDKFRD-----PLLIKTEDHGF-----DIVE 180
           DFAIPFWNWD P GM  M + Y  P+SSLYD+ RD     P L+     G      +IV+
Sbjct: 67  DFAIPFWNWDAPPGMC-MPKIYTKPSSSLYDENRDKCHQPPKLLNLNYGGKEVKDPEIVD 126

Query: 181 CKLHWMKQRMNVTSARRFLGNIVEVGKVP 196
             L WM  +M+V +A+ FLG +++ G  P
Sbjct: 127 FNLRWMNDQMSVETAKEFLGKVIKRGNGP 152

HSP 2 Score: 34.7 bits (78), Expect = 3.6e+02
Identity = 14/24 (58.33%), Postives = 20/24 (83.33%), Query Frame = 1


Query: 281 VKMKPKLGGESVSIGGITAQLQPV 305
           VK++P +GGE V++GGI  QL+PV
Sbjct: 700 VKIEPMVGGEGVTLGGIKIQLEPV 723

HSP 3 Score: 34.3 bits (77), Expect = 4.6e+02
Identity = 13/14 (92.86%), Postives = 13/14 (92.86%), Query Frame = 1


Query: 201 GRDPLFYAHHVNVD 215
           GRDPLFYAHH NVD
Sbjct: 190 GRDPLFYAHHANVD 203

HSP 4 Score: 236.9 bits (603), Expect = 4.7e-59
Identity = 123/228 (53.95%), Postives = 144/228 (63.16%), Query Frame = 1


Query: 88  VHSNALFFPPHRAYLYFFERFLSSLIGNPDFAIPFWNWDTPDGMYNMTEPYNDPNSSLYD 147
           VHSNALFFP HRAYLYFFER L   IGNP+FAIPFWNWD+P GMY M E YN P+SSLYD
Sbjct: 179 VHSNALFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMY-MPEFYNRPSSSLYD 238

Query: 148 KFRDPL-----LI------KTEDHGFDIVECKLHWMKQRMNVTSARRFLGNIVEVGKVPT 207
             RDP      LI      KTEDH FD+V+C L WM  RM V S R FLG +++ G+ P 
Sbjct: 239 HLRDPRHDPHKLIYLNYNGKTEDHSFDVVDCNLRWMNDRMQVQSPRSFLGRVLKAGETPN 298

Query: 208 HNDMGR-------------------------------DPLFYAHHVNVDGMWKIWKEM-G 267
             DMG                                DPLFYAHH N+D +W IWK + G
Sbjct: 299 PTDMGTIEQSPHNNIHDWLGTKTPHFEDMGSFYSAALDPLFYAHHANIDRLWNIWKTLDG 358

Query: 268 N-QDHTDEGWVNSTFVFYDENQNAVRIKVSDCLDSKKLGYDYEKRIIP 272
           N +DH D  W+NS+FVFYDEN+NAV IKVSDCLD++KLGY Y++  +P
Sbjct: 359 NPKDHIDPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLGYVYQEVPLP 405

[Close]

BLAST of Csa1G073100.1 vs. NCBI nr
Match: gi|449457811|ref|XP_004146641.1| (PREDICTED: polyphenol oxidase, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 33.9 bits (76), Expect = 6.1e+02
Identity = 13/24 (54.17%), Postives = 20/24 (83.33%), Query Frame = 1


Query: 281 VKMKPKLGGESVSIGGITAQLQPV 305
           VK++P +GGE V++GGI  QL+P+
Sbjct: 534 VKIEPMVGGEGVTVGGIKIQLEPL 557

HSP 2 Score: 221.9 bits (564), Expect = 1.6e-54
Identity = 104/104 (100.00%), Postives = 104/104 (100.00%), Query Frame = 1


Query: 201 GRDPLFYAHHVNVDGMWKIWKEMGNQDHTDEGWVNSTFVFYDENQNAVRIKVSDCLDSKK 260
           GRDPLFYAHHVNVDGMWKIWKEMGNQDHTDEGWVNSTFVFYDENQNAVRIKVSDCLDSKK
Sbjct: 8   GRDPLFYAHHVNVDGMWKIWKEMGNQDHTDEGWVNSTFVFYDENQNAVRIKVSDCLDSKK 67

Query: 261 LGYDYEKRIIPAASNEAKFPVKMKPKLGGESVSIGGITAQLQPV 305
           LGYDYEKRIIPAASNEAKFPVKMKPKLGGESVSIGGITAQLQPV
Sbjct: 68  LGYDYEKRIIPAASNEAKFPVKMKPKLGGESVSIGGITAQLQPV 111

[Close]

BLAST of Csa1G073100.1 vs. NCBI nr
Match: gi|590624783|ref|XP_007025702.1| (Uncharacterized protein TCM_029922 [Theobroma cacao])

HSP 1 Score: 179.9 bits (455), Expect = 6.8e-42
Identity = 102/233 (43.78%), Postives = 133/233 (57.08%), Query Frame = 1


Query: 86  VQVHSNALFFPPHRAYLYFFERFLSSLIGNPDFAIPFWNWDTPDGMYNMTEPYNDPNSSL 145
           +QVH++ LFFP HR YLYFFE+ L  LI +P FAIPFWNWD+P GM  M   Y +PNS L
Sbjct: 202 LQVHNSWLFFPFHRYYLYFFEKILGKLIDDPTFAIPFWNWDSPAGM-QMPAIYANPNSPL 261

Query: 146 YDKFRDP-----LLIKTEDHGF-------DIVECKLHWMKQRM--NVTSARRFLGNIVEV 205
           YD+ R+P      L+  + +G        D +   L+ M ++M  N  +A+ FLGN    
Sbjct: 262 YDENRNPNHQPPTLLDLDFNGTEESSSTRDQISSNLNVMYRQMVSNGKTAKLFLGNAYRA 321

Query: 206 G-------------------------KVPTHNDMG------RDPLFYAHHVNVDGMWKIW 265
           G                         + P   DMG      RDP+FYAHH NVD MW +W
Sbjct: 322 GDQPDPGAGSLENIPHGPIHIWCGDNRQPNLEDMGNFYSAGRDPIFYAHHSNVDRMWSVW 381

Query: 266 KEMGNQ--DHTDEGWVNSTFVFYDENQNAVRIKVSDCLDSKKLGYDYEKRIIP 272
           K +G +  D TD  W++S F+FYDEN N VR+KV DCLD++K+GYDY+K  IP
Sbjct: 382 KTLGGKRTDFTDSDWLDSGFLFYDENANLVRVKVRDCLDTRKMGYDYQKVDIP 433

The following BLAST results are available for this feature:

BLAST of Csa1G073100.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PPO_VICFA	9.2e-39	42.00	Polyphenol oxidase A1, chloroplastic OS=Vicia faba PE=1 SV=1	[more]
PPO1_IPOBA	2.7e-38	37.55	Polyphenol oxidase I, chloroplastic OS=Ipomoea batatas GN=co-1 PE=1 SV=1	[more]
PPOF_SOLLC	1.6e-35	40.09	Polyphenol oxidase F, chloroplastic OS=Solanum lycopersicum PE=3 SV=1	[more]
PPOE_SOLLC	2.1e-35	39.74	Polyphenol oxidase E, chloroplastic OS=Solanum lycopersicum PE=3 SV=1	[more]
PPOB_SOLLC	5.2e-34	34.98	Polyphenol oxidase B, chloroplastic OS=Solanum lycopersicum PE=3 SV=1	[more]

BLAST of Csa1G073100.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LRV2_CUCSA	7.1e-179	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G073100 PE=4 SV=1	[more]
A0A0A0LUI1_CUCSA	3.3e-59	53.95	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G073090 PE=4 SV=1	[more]
A0A0A0LUI1_CUCSA	4.2e+02	54.17	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G073090 PE=4 SV=1	[more]
M0TW39_MUSAM	3.4e-40	45.88	Uncharacterized protein OS=Musa acuminata subsp. malaccensis PE=4 SV=1	[more]
A0A0J8EMT5_BETVU	5.8e-40	39.48	Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_8g183910 PE=4 S...	[more]

BLAST of Csa1G073100.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700209554\|gb\|KGN64650.1\|	1.0e-178	100.00	hypothetical protein Csa_1G073100 [Cucumis sativus]	[more]
gi\|659068847\|ref\|XP_008446626.1\|	2.5e-60	53.68	PREDICTED: polyphenol oxidase I, chloroplastic-like [Cucumis melo]	[more]
gi\|659068847\|ref\|XP_008446626.1\|	2.4e-23	46.31	PREDICTED: polyphenol oxidase I, chloroplastic-like [Cucumis melo]	[more]
gi\|449457811\|ref\|XP_004146641.1\|	6.1e+02	54.17	PREDICTED: polyphenol oxidase, chloroplastic-like [Cucumis sativus]	[more]
gi\|590624783\|ref\|XP_007025702.1\|	6.8e-42	43.78	Uncharacterized protein TCM_029922 [Theobroma cacao]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR002227	Tyrosinase_Cu-bd
IPR008922	Unchr_di-copper_centre
IPR022739	Polyphenol_oxidase_cen

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity
GO:0004097	catechol oxidase activity

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0006570	tyrosine metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004097	catechol oxidase activity
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0046872	metal ion binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Csa1G073100	Csa1G073100	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Csa1G073100.1	Csa1G073100.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csa1G073100.1.cds5	Csa1G073100.1.cds5	CDS
Csa1G073100.1.cds4	Csa1G073100.1.cds4	CDS
Csa1G073100.1.cds3	Csa1G073100.1.cds3	CDS
Csa1G073100.1.cds2	Csa1G073100.1.cds2	CDS
Csa1G073100.1.cds1	Csa1G073100.1.cds1	CDS

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002227	Tyrosinase copper-binding domain	PFAM	PF00264	Tyrosinase	coord: 74..135 score: 7.3E-13coord: 201..222 score: 5.
IPR002227	Tyrosinase copper-binding domain	PROSITE	PS00498	TYROSINASE_2	coord: 203..214 scor
IPR008922	Uncharacterised domain, di-copper centre	GENE3D	G3DSA:1.10.1280.10		coord: 85..152 score: 3.9E-18coord: 201..267 score: 6.5
IPR008922	Uncharacterised domain, di-copper centre	unknown	SSF48056	Di-copper centre-containing domain	coord: 70..266 score: 4.06
IPR022739	Polyphenol oxidase, central domain	PFAM	PF12142	PPO1_DWL	coord: 227..276 score: 5.1
None	No IPR available	PANTHER	PTHR11474	TYROSINASE	coord: 85..266 score: 1.3
None	No IPR available	PANTHER	PTHR11474:SF33	SUBFAMILY NOT NAMED	coord: 85..266 score: 1.3