CsGy7G016780.1 (mRNA) Cucumber (Gy14) v2

NameCsGy7G016780.1
TypemRNA
OrganismCucumis sativus (Cucumber (Gy14) v2)
DescriptionnifU-like protein 1, chloroplastic
LocationChr7 : 20302206 .. 20304363 (+)
Sequence length663
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTCCTTAACTACCTCCGGCCTCCTCAAAACCCCTCTTAAGAATTCCCAATCTCCTGCAAATTCTCTCCAATATCCACCTTTTGCTTATGGGCAACATCAACTGCCCGTTTCCCTCAAAAGGGCTTTTCCCGGACGAGCCATTAGAGCTTCACCTTCGAATTCGGGTCCATCCACGACCTCCTCACCAGGGCTGTACTCCGCTCAGAAATTTGAATTGACCATTGGAAACGTGGACTTGGTTCTTGAGGACGTAAGGCCTTATCTAATTGCAGATGGAGGAAATGTCGATGTTGTTTCTGTTGAAGATGGAGTAGTTTCCCTCAAACTCGTTGGTGGGTAGATTTCTTGTCTCGTTGAATTGAATGGGTTTGTTTTAATTTCCCTTGTTAATTTAATTTTTTGTGTTGAAATTTTTGTTACTTATAAATTGGTTGTTGTTGGGGTATGTTCTTTGGTGTTTCTATAGTTTAGCAAATTGTGTTGAATATCTTTTACACATTATGGTATTTAGTAGTATCTGCACTTTAGAGATTTGAGTCTAATTCATTTCTTTCATCGAAGCAATCCTGGGATCGTATGGTTTAGGACAAAGTTGATATCTTTGTGATTCTCGGTCTGAGTATGCCCCTTCAGCCCATCTCTTTTTACATAGCCAAATAGTTGGTGTATGCTTTCTCGACGCTGGCCCGATAGAAGTGATTCCTTTTTCTCAAAAAGAATGGTCTCTTTCTTAGCATTACTGTAACCCATTTGATCTTAGTCGTCTTGTGTGGAAGGAGCGAGATGACAGAGATTTTGCTCTTCTCACCCCTGTCCGACCACCCGATTGGTGAAGACTCGGAACAAACCACCCCGCTTTGGGAGACAACGAAGACTTTACTCGATGTCTTGATCCATCCGTAGTCTGCTAATCTGATCCCAACGTCTTTAATTCTGACGAGCTTGTCTCGGAGAGATCTCTATCTTTTGTTTGTTACACAACACTGAAGGCTCCACTAGAAAAGGAAGGAAAAAGGAAAATACCTCCAATGAGAAATCTCAAGATCAAGATTAGTAGTGCTGCAGTTCTATTTACAATCTTAAACTTTCCTAACTACAATCTTCCTCCATTAGCACTGAATCTATTGGAAATTTTGAACAGTTTAGACTTGGGCAGATGGATATGATTTTCCTTTCCTTATATGAGGGTCTTTCCAATGAAAAGGATTTACCAGAGAATAGATGATTTGATATCTATCTTTAAAGAAAACCACGAAAAGGATCCTTAATTTCACTTGTTTGTGAACACTTTTAACCAATCTTGTGGGAGATTTGGTATATATCTAACAGGAATGTTTCTCAGGACATGGGTGGGGTTCTCATGACATTTGAAGGGACTGCAGACTGATGTGGCCTTTCGGTTTCTTTCCTGCATATACTAGTTGGAGTACATTTGTTTTGTTGTCTTGGTCTAGGTAAGTTTTGGACAGCACCATCATCTTGTACATGTTACTGGTGTGTTTTATCTGTTTCTTATCAAGTAAGGTGAAAAAGGTCTCCTTGAACTAAATGGCAAAAGGAAGTCTTGTAACCCTCACTTTCATCGAAAAGGGTGTGAGTAAGAACTTTGTTGATTTGATGTTTAACTGCTTTGTATTCCCATGAAGTCATGAACAAAAAGTAATAATGATAAACATTTTTAACTAAAAATGTTTGAATCGATCTTCAGGAGCATGCGGTAGCTGCCCTAGCTCAACAACGACCATGAAGATGGGGATCGAACGTGTACTAAAGGAGAAGTTTGGAGATTCAGTGAAGGAAATATGCCAAGTATATGATGAAGAACCAAAGGAGACTACACCTGAGGTGAGTTTTCTGCAGACGAGCGAATACAAAATTGGCTGATGTCAAAATTCAAACAAAAACAAAGTGAGCTGATTTAGTTAGGCAATTTAGTATTAATCCTGGATGTTGCACAAATTCAGGCAGTAAACAGTCACCTTGACATATTGAGGCCAGCCATAAGGAACTACGGTGGGAGCGTGGAAGTAATATCAATTAATGGTGGCGATTGCCTGGTCAAGTATGAGGGTCCTGAATCTATTGGTACAGGAGTTAAAGCAGCCATTAAGGAGAGATTCCCAGATATTACAAACGTTGTGTTTTCAAGCTAA

mRNA sequence

ATGGCGTCCTTAACTACCTCCGGCCTCCTCAAAACCCCTCTTAAGAATTCCCAATCTCCTGCAAATTCTCTCCAATATCCACCTTTTGCTTATGGGCAACATCAACTGCCCGTTTCCCTCAAAAGGGCTTTTCCCGGACGAGCCATTAGAGCTTCACCTTCGAATTCGGGTCCATCCACGACCTCCTCACCAGGGCTGTACTCCGCTCAGAAATTTGAATTGACCATTGGAAACGTGGACTTGGTTCTTGAGGACGTAAGGCCTTATCTAATTGCAGATGGAGGAAATGTCGATGTTGTTTCTGTTGAAGATGGAGTAGTTTCCCTCAAACTCGTTGGAGCATGCGGTAGCTGCCCTAGCTCAACAACGACCATGAAGATGGGGATCGAACGTGTACTAAAGGAGAAGTTTGGAGATTCAGTGAAGGAAATATGCCAAGTATATGATGAAGAACCAAAGGAGACTACACCTGAGGCAGTAAACAGTCACCTTGACATATTGAGGCCAGCCATAAGGAACTACGGTGGGAGCGTGGAAGTAATATCAATTAATGGTGGCGATTGCCTGGTCAAGTATGAGGGTCCTGAATCTATTGGTACAGGAGTTAAAGCAGCCATTAAGGAGAGATTCCCAGATATTACAAACGTTGTGTTTTCAAGCTAA

Coding sequence (CDS)

ATGGCGTCCTTAACTACCTCCGGCCTCCTCAAAACCCCTCTTAAGAATTCCCAATCTCCTGCAAATTCTCTCCAATATCCACCTTTTGCTTATGGGCAACATCAACTGCCCGTTTCCCTCAAAAGGGCTTTTCCCGGACGAGCCATTAGAGCTTCACCTTCGAATTCGGGTCCATCCACGACCTCCTCACCAGGGCTGTACTCCGCTCAGAAATTTGAATTGACCATTGGAAACGTGGACTTGGTTCTTGAGGACGTAAGGCCTTATCTAATTGCAGATGGAGGAAATGTCGATGTTGTTTCTGTTGAAGATGGAGTAGTTTCCCTCAAACTCGTTGGAGCATGCGGTAGCTGCCCTAGCTCAACAACGACCATGAAGATGGGGATCGAACGTGTACTAAAGGAGAAGTTTGGAGATTCAGTGAAGGAAATATGCCAAGTATATGATGAAGAACCAAAGGAGACTACACCTGAGGCAGTAAACAGTCACCTTGACATATTGAGGCCAGCCATAAGGAACTACGGTGGGAGCGTGGAAGTAATATCAATTAATGGTGGCGATTGCCTGGTCAAGTATGAGGGTCCTGAATCTATTGGTACAGGAGTTAAAGCAGCCATTAAGGAGAGATTCCCAGATATTACAAACGTTGTGTTTTCAAGCTAA

Protein sequence

MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPSTTSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEVISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS
BLAST of CsGy7G016780.1 vs. NCBI nr
Match: XP_004135981.1 (PREDICTED: nifU-like protein 1, chloroplastic [Cucumis sativus] >KGN45036.1 hypothetical protein Csa_7G412870 [Cucumis sativus])

HSP 1 Score: 434.5 bits (1116), Expect = 2.2e-118
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 0

Query: 1   MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST
Sbjct: 1   MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60

Query: 61  TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS
Sbjct: 61  TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180

Query: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 221
           ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS
Sbjct: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 220

BLAST of CsGy7G016780.1 vs. NCBI nr
Match: XP_008451442.1 (PREDICTED: nifU-like protein 1, chloroplastic [Cucumis melo])

HSP 1 Score: 425.6 bits (1093), Expect = 1.0e-115
Identity = 214/220 (97.27%), Postives = 218/220 (99.09%), Query Frame = 0

Query: 1   MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLTTSGLLKTPLKNSQSPAN  QYPPFAYGQHQLPVSLKRAFPGRA+RASPSNSGPST
Sbjct: 1   MASLTTSGLLKTPLKNSQSPANYFQYPPFAYGQHQLPVSLKRAFPGRAVRASPSNSGPST 60

Query: 61  TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           +SSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVN+HLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNNHLDILRPAIRNYGGSVEV 180

Query: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 221
           ISINGG+CLVKYEGPESIGTGVKAAIKERFPDITNVVFSS
Sbjct: 181 ISINGGNCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 220

BLAST of CsGy7G016780.1 vs. NCBI nr
Match: XP_022144330.1 (nifU-like protein 1, chloroplastic [Momordica charantia])

HSP 1 Score: 386.3 bits (991), Expect = 6.7e-104
Identity = 198/219 (90.41%), Postives = 204/219 (93.15%), Query Frame = 0

Query: 1   MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLTTSGLLKTP   SQ PAN  QYPP A+ QHQ P SLKR  P R I+AS SNSGPST
Sbjct: 1   MASLTTSGLLKTPPCYSQIPANYRQYPPSAHRQHQGPNSLKRVLPRRGIKASASNSGPST 60

Query: 61  TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           +SSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLD+LRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDVLRPAIRNYGGSVEV 180

Query: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFS 220
           ISI+GGDCLVKYEGPESIGTG+KAAIKERFPDITNVVFS
Sbjct: 181 ISIDGGDCLVKYEGPESIGTGIKAAIKERFPDITNVVFS 219

BLAST of CsGy7G016780.1 vs. NCBI nr
Match: XP_023547622.1 (nifU-like protein 1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 380.6 bits (976), Expect = 3.7e-102
Identity = 192/220 (87.27%), Postives = 205/220 (93.18%), Query Frame = 0

Query: 1   MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLT SGLLKTPL  SQ+ AN  QYPPF++GQHQ+PV L RA P +AIRAS S+SGPST
Sbjct: 1   MASLTISGLLKTPLYYSQALANHRQYPPFSFGQHQIPVCLNRALPSQAIRASASSSGPST 60

Query: 61  TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           +SSPGLYSAQKFELT  NVDLVLEDVRPYLIADGGNVDVVSVEDG+VSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKE T EAVNSHLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180

Query: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 221
           ISI+GG+C+VKYEGPESIGTG+KAAIKE+FPDITNVVFSS
Sbjct: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS 220

BLAST of CsGy7G016780.1 vs. NCBI nr
Match: XP_022991837.1 (nifU-like protein 1, chloroplastic [Cucurbita maxima])

HSP 1 Score: 380.2 bits (975), Expect = 4.8e-102
Identity = 193/220 (87.73%), Postives = 205/220 (93.18%), Query Frame = 0

Query: 1   MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLT SGLLKTPL  SQ+ AN  QYPP A+GQHQ+PVSL RA P ++IRAS SNSGPST
Sbjct: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRALPRQSIRASASNSGPST 60

Query: 61  TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           +SSPGLYSAQKFELTI NVDLVLEDVRPYLIADGGNVDVVSVEDG+VSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKE T EAVNSHLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180

Query: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 221
           ISI+GG+C+V YEGPESIGTG+KAAIKE+FPDITNVVFSS
Sbjct: 181 ISIDGGNCVVMYEGPESIGTGIKAAIKEKFPDITNVVFSS 220

BLAST of CsGy7G016780.1 vs. TAIR10
Match: AT4G01940.1 (NFU domain protein 1)

HSP 1 Score: 248.8 bits (634), Expect = 3.0e-66
Identity = 142/230 (61.74%), Postives = 169/230 (73.48%), Query Frame = 0

Query: 1   MASLTT--SGLLKTPLKNSQS----PANSLQYPPFA-YGQHQLPVSLKRAFPGRAIRASP 60
           MASL T  SG  +  +K+S +    P  S Q P F  +   +  +S    F   AI  S 
Sbjct: 2   MASLATSISGSFRILVKSSSTRNGFPVISDQNPSFVLFANKRRHISRTAIFHRSAISGSS 61

Query: 61  SNSGPSTTS---SPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLK 120
                S  +   S GLYSAQ F+LT  NVDLVLEDVRP+LI+DGGNVDVVSVEDGVVSLK
Sbjct: 62  QGEKISPLASGVSSGLYSAQTFDLTPQNVDLVLEDVRPFLISDGGNVDVVSVEDGVVSLK 121

Query: 121 LVGACGSCPSSTTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPA 180
           L GAC SCPSS+TTM MGIERVLKEKFGD++K+I QV+DEE K+ T EAVN+HLDILRPA
Sbjct: 122 LQGACTSCPSSSTTMTMGIERVLKEKFGDALKDIRQVFDEEVKQITVEAVNAHLDILRPA 181

Query: 181 IRNYGGSVEVISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 221
           I+NYGGSVEV+S+ G DC+VKY GPESIG G++AAIKE+F DI+NV F+S
Sbjct: 182 IKNYGGSVEVLSVEGEDCVVKYVGPESIGMGIQAAIKEKFKDISNVTFTS 231

BLAST of CsGy7G016780.1 vs. TAIR10
Match: AT5G49940.1 (NIFU-like protein 2)

HSP 1 Score: 97.8 bits (242), Expect = 8.7e-21
Identity = 57/148 (38.51%), Postives = 84/148 (56.76%), Query Frame = 0

Query: 74  LTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL+++RPYL++DGGNV +  ++  +V +KL GACGSCPSST TMKMGIER L
Sbjct: 85  LTEENVESVLDEIRPYLMSDGGNVALHEIDGNIVRVKLQGACGSCPSSTMTMKMGIERRL 144

Query: 134 KEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRP-AIRNYGGSVEVISINGGDCLVKY 193
            EK  + V       +E   E   E +   L+ +RP  I    GS++++ I      ++ 
Sbjct: 145 MEKIPEIVAVEALPDEETGLELNEENIEKVLEEIRPYLIGTADGSLDLVEIEDPIVKIRI 204

Query: 194 EGPE----SIGTGVKAAIKERFPDITNV 217
            GP     ++   V   ++E+ P I  V
Sbjct: 205 TGPAAGVMTVRVAVTQKLREKIPSIAAV 232

BLAST of CsGy7G016780.1 vs. TAIR10
Match: AT4G25910.1 (NFU domain protein 3)

HSP 1 Score: 89.7 bits (221), Expect = 2.4e-18
Identity = 56/139 (40.29%), Postives = 83/139 (59.71%), Query Frame = 0

Query: 74  LTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL++VRP L+ADGGNV +  ++  VV LKL GACGSCPSS+ T+KMGIE  L
Sbjct: 84  LTEENVERVLDEVRPSLMADGGNVALHEIDGLVVVLKLQGACGSCPSSSMTLKMGIESRL 143

Query: 134 KEKFGDSVKEICQVYDEEP--KETTPEAVNSHLDILRPAIRNY-GGSVEVISINGGDCLV 193
           ++K  + +  + Q  + E    E   E +   L  LRP +    GG +E++ I+G    V
Sbjct: 144 RDKIPE-IMSVEQFLESETGGLELNDENIEKVLSELRPYLSGTGGGGLELVEIDGYVVKV 203

Query: 194 KYEGPESIGTGVKAAIKER 210
           +  GP +    V+ A+ ++
Sbjct: 204 RLTGPAAGVMTVRVALTQK 221

BLAST of CsGy7G016780.1 vs. TAIR10
Match: AT3G20970.1 (NFU domain protein 4)

HSP 1 Score: 54.7 bits (130), Expect = 8.4e-08
Identity = 32/85 (37.65%), Postives = 50/85 (58.82%), Query Frame = 0

Query: 75  TIGNVDLVLED-VRPYLIADGGNVDVVSV--EDGVVSLKLVGACGSCPSSTTTMKMGIER 134
           T+  +  +LE  +RP +  DGG+++      E G+V L++ GAC  CPSS+ T+K GIE 
Sbjct: 192 TVAMIKELLETRIRPAVQDDGGDIEYCGFDPESGIVKLRMQGACSGCPSSSVTLKSGIEN 251

Query: 135 VLKEKFGDSVKEICQVYDEEPKETT 157
           +L   +   VK + Q +D E +E T
Sbjct: 252 MLMH-YVSEVKGVEQEFDGEDEEGT 275

BLAST of CsGy7G016780.1 vs. TAIR10
Match: AT1G51390.1 (NFU domain protein 5)

HSP 1 Score: 53.1 bits (126), Expect = 2.5e-07
Identity = 31/85 (36.47%), Postives = 50/85 (58.82%), Query Frame = 0

Query: 75  TIGNVDLVLED-VRPYLIADGGNVDV--VSVEDGVVSLKLVGACGSCPSSTTTMKMGIER 134
           T+  +  +LE  +RP +  DGG+++      E G+V L++ GAC  CPSS+ T+K GIE 
Sbjct: 187 TVAMIKELLETRIRPSVQDDGGDIEYCGFDTETGIVKLRMQGACSGCPSSSVTLKSGIEN 246

Query: 135 VLKEKFGDSVKEICQVYDEEPKETT 157
           +L   +   VK + Q +D E + T+
Sbjct: 247 MLMH-YVSEVKGVEQEFDGEEEGTS 270

BLAST of CsGy7G016780.1 vs. Swiss-Prot
Match: sp|Q93W77|NIFU1_ARATH (NifU-like protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU1 PE=1 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 5.5e-65
Identity = 142/230 (61.74%), Postives = 169/230 (73.48%), Query Frame = 0

Query: 1   MASLTT--SGLLKTPLKNSQS----PANSLQYPPFA-YGQHQLPVSLKRAFPGRAIRASP 60
           MASL T  SG  +  +K+S +    P  S Q P F  +   +  +S    F   AI  S 
Sbjct: 2   MASLATSISGSFRILVKSSSTRNGFPVISDQNPSFVLFANKRRHISRTAIFHRSAISGSS 61

Query: 61  SNSGPSTTS---SPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLK 120
                S  +   S GLYSAQ F+LT  NVDLVLEDVRP+LI+DGGNVDVVSVEDGVVSLK
Sbjct: 62  QGEKISPLASGVSSGLYSAQTFDLTPQNVDLVLEDVRPFLISDGGNVDVVSVEDGVVSLK 121

Query: 121 LVGACGSCPSSTTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPA 180
           L GAC SCPSS+TTM MGIERVLKEKFGD++K+I QV+DEE K+ T EAVN+HLDILRPA
Sbjct: 122 LQGACTSCPSSSTTMTMGIERVLKEKFGDALKDIRQVFDEEVKQITVEAVNAHLDILRPA 181

Query: 181 IRNYGGSVEVISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 221
           I+NYGGSVEV+S+ G DC+VKY GPESIG G++AAIKE+F DI+NV F+S
Sbjct: 182 IKNYGGSVEVLSVEGEDCVVKYVGPESIGMGIQAAIKEKFKDISNVTFTS 231

BLAST of CsGy7G016780.1 vs. Swiss-Prot
Match: sp|Q84LK7|NIFU1_ORYSJ (NifU-like protein 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NIFU1 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.6e-19
Identity = 70/179 (39.11%), Postives = 98/179 (54.75%), Query Frame = 0

Query: 45  PGRAIRASPSNSGPSTT--SSPGL--YSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVV 104
           P   + A+P++  P     +  GL   +A +  LT GNV+ VL+ VRPYL ADGG+V + 
Sbjct: 42  PATRLVAAPNHQRPPLVVGAIAGLDPVTAVQLPLTAGNVESVLDQVRPYLTADGGDVALH 101

Query: 105 SVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKEKFGDSVKEICQVYDEEP-KETTPEA 164
            +   VV LKL GACGSCPSS  T+K GIER L EK  D V  +  V D+E   E   E 
Sbjct: 102 EIAGNVVRLKLQGACGSCPSSLITIKRGIERRLMEKIPD-VAAVEPVTDKETGLELNEEN 161

Query: 165 VNSHLDILRPAIRNY-GGSVEVISINGGDCLVKYEGPESIGTGVKAAI----KERFPDI 214
           V   L+ +RP +    GG ++ + I G    V+  GP ++   V+ A+    +E+ P I
Sbjct: 162 VEKVLNEIRPYLAGTGGGGLQFLMIKGPIVKVRLTGPAAVVRTVRIAVSKKLREKIPSI 219

BLAST of CsGy7G016780.1 vs. Swiss-Prot
Match: sp|Q93W20|NIFU2_ARATH (NifU-like protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU2 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.6e-19
Identity = 57/148 (38.51%), Postives = 84/148 (56.76%), Query Frame = 0

Query: 74  LTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL+++RPYL++DGGNV +  ++  +V +KL GACGSCPSST TMKMGIER L
Sbjct: 85  LTEENVESVLDEIRPYLMSDGGNVALHEIDGNIVRVKLQGACGSCPSSTMTMKMGIERRL 144

Query: 134 KEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRP-AIRNYGGSVEVISINGGDCLVKY 193
            EK  + V       +E   E   E +   L+ +RP  I    GS++++ I      ++ 
Sbjct: 145 MEKIPEIVAVEALPDEETGLELNEENIEKVLEEIRPYLIGTADGSLDLVEIEDPIVKIRI 204

Query: 194 EGPE----SIGTGVKAAIKERFPDITNV 217
            GP     ++   V   ++E+ P I  V
Sbjct: 205 TGPAAGVMTVRVAVTQKLREKIPSIAAV 232

BLAST of CsGy7G016780.1 vs. Swiss-Prot
Match: sp|Q84RQ7|NIFU3_ARATH (NifU-like protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU3 PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 4.3e-17
Identity = 56/139 (40.29%), Postives = 83/139 (59.71%), Query Frame = 0

Query: 74  LTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL++VRP L+ADGGNV +  ++  VV LKL GACGSCPSS+ T+KMGIE  L
Sbjct: 84  LTEENVERVLDEVRPSLMADGGNVALHEIDGLVVVLKLQGACGSCPSSSMTLKMGIESRL 143

Query: 134 KEKFGDSVKEICQVYDEEP--KETTPEAVNSHLDILRPAIRNY-GGSVEVISINGGDCLV 193
           ++K  + +  + Q  + E    E   E +   L  LRP +    GG +E++ I+G    V
Sbjct: 144 RDKIPE-IMSVEQFLESETGGLELNDENIEKVLSELRPYLSGTGGGGLELVEIDGYVVKV 203

Query: 194 KYEGPESIGTGVKAAIKER 210
           +  GP +    V+ A+ ++
Sbjct: 204 RLTGPAAGVMTVRVALTQK 221

BLAST of CsGy7G016780.1 vs. Swiss-Prot
Match: sp|O32119|YUTI_BACSU (Putative nitrogen fixation protein YutI OS=Bacillus subtilis (strain 168) OX=224308 GN=yutI PE=3 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 6.4e-13
Identity = 37/70 (52.86%), Postives = 53/70 (75.71%), Query Frame = 0

Query: 79  VDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKEKFG 138
           V  VL+ +RP+L+ DGG+ ++V V++G+V L+L+GACGSCPSST T+K GIER L E+  
Sbjct: 43  VQEVLDKLRPFLLRDGGDCELVDVDEGIVKLRLLGACGSCPSSTITLKAGIERALLEEV- 102

Query: 139 DSVKEICQVY 149
             V E+ QV+
Sbjct: 103 PGVVEVEQVF 111

BLAST of CsGy7G016780.1 vs. TrEMBL
Match: tr|A0A0A0KB98|A0A0A0KB98_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G412870 PE=4 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 1.4e-118
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 0

Query: 1   MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST
Sbjct: 1   MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60

Query: 61  TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS
Sbjct: 61  TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180

Query: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 221
           ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS
Sbjct: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 220

BLAST of CsGy7G016780.1 vs. TrEMBL
Match: tr|A0A1S3BQX1|A0A1S3BQX1_CUCME (nifU-like protein 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492737 PE=4 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 6.6e-116
Identity = 214/220 (97.27%), Postives = 218/220 (99.09%), Query Frame = 0

Query: 1   MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLTTSGLLKTPLKNSQSPAN  QYPPFAYGQHQLPVSLKRAFPGRA+RASPSNSGPST
Sbjct: 1   MASLTTSGLLKTPLKNSQSPANYFQYPPFAYGQHQLPVSLKRAFPGRAVRASPSNSGPST 60

Query: 61  TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           +SSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVN+HLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNNHLDILRPAIRNYGGSVEV 180

Query: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 221
           ISINGG+CLVKYEGPESIGTGVKAAIKERFPDITNVVFSS
Sbjct: 181 ISINGGNCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 220

BLAST of CsGy7G016780.1 vs. TrEMBL
Match: tr|M5XUC8|M5XUC8_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_3G007000 PE=4 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 7.2e-78
Identity = 158/219 (72.15%), Postives = 179/219 (81.74%), Query Frame = 0

Query: 1   MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLT +GL KTP   S+ PA S QYP   + Q     S    F   AIRAS + S  + 
Sbjct: 1   MASLTATGLPKTPTPFSKIPATSHQYPQSVFVQRHRLTSQGSIFVKSAIRAS-NPSAAAE 60

Query: 61  TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           +SSPGLYSA+K+ELT+ NVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKL GACGSCPS
Sbjct: 61  SSSPGLYSAKKYELTVPNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLQGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGD++K+I QVYDEE KETT EAVN HLDILRPAI+N+GGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDALKDIQQVYDEENKETTVEAVNGHLDILRPAIKNFGGSVEV 180

Query: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFS 220
           +S+ GGDC V Y GP+SIG+G+KAAIKE+FPDI NVVF+
Sbjct: 181 LSVEGGDCHVNYVGPDSIGSGIKAAIKEKFPDIVNVVFT 218

BLAST of CsGy7G016780.1 vs. TrEMBL
Match: tr|A0A140B480|A0A140B480_PRUPE (NFU domain protein 1 OS=Prunus persica var. persica OX=323852 GN=NFU1 PE=2 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 7.2e-78
Identity = 158/219 (72.15%), Postives = 179/219 (81.74%), Query Frame = 0

Query: 1   MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLT +GL KTP   S+ PA S QYP   + Q     S    F   AIRAS + S  + 
Sbjct: 1   MASLTATGLPKTPTPFSKIPATSHQYPQSVFVQRHRLTSQGSIFVKSAIRAS-NPSAAAE 60

Query: 61  TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           +SSPGLYSA+K+ELT+ NVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKL GACGSCPS
Sbjct: 61  SSSPGLYSAKKYELTVPNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLQGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGD++K+I QVYDEE KETT EAVN HLDILRPAI+N+GGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDALKDIQQVYDEENKETTVEAVNGHLDILRPAIKNFGGSVEV 180

Query: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFS 220
           +S+ GGDC V Y GP+SIG+G+KAAIKE+FPDI NVVF+
Sbjct: 181 LSVEGGDCHVNYVGPDSIGSGIKAAIKEKFPDIVNVVFT 218

BLAST of CsGy7G016780.1 vs. TrEMBL
Match: tr|A0A2I4DNY7|A0A2I4DNY7_9ROSI (nifU-like protein 1, chloroplastic OS=Juglans regia OX=51240 GN=LOC108982048 PE=4 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 9.4e-78
Identity = 158/219 (72.15%), Postives = 175/219 (79.91%), Query Frame = 0

Query: 1   MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLT +GL KTP  + +  + + QYP F   +     S +R F   AIRA  SN     
Sbjct: 1   MASLTATGLPKTPTSSPKFSSKTHQYPQFISLKEAQRASQRRLFSKTAIRA--SNPSAPA 60

Query: 61  TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
            SSPGLYSAQKFELT  NVD VLEDVRPYLIADGGNVDVVSVEDG+VSLKL GACGSCPS
Sbjct: 61  GSSPGLYSAQKFELTARNVDSVLEDVRPYLIADGGNVDVVSVEDGIVSLKLQGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGD+VK+I QVYDEE   TT EAVN HLD+LRPAI+NYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDAVKDIRQVYDEEVGGTTVEAVNGHLDVLRPAIKNYGGSVEV 180

Query: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFS 220
           +SI GGDCLVKY GPESIGTG+KAAIKE+FPD+ NVVF+
Sbjct: 181 LSIEGGDCLVKYVGPESIGTGIKAAIKEKFPDVVNVVFT 217

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004135981.12.2e-118100.00PREDICTED: nifU-like protein 1, chloroplastic [Cucumis sativus] >KGN45036.1 hypo... [more]
XP_008451442.11.0e-11597.27PREDICTED: nifU-like protein 1, chloroplastic [Cucumis melo][more]
XP_022144330.16.7e-10490.41nifU-like protein 1, chloroplastic [Momordica charantia][more]
XP_023547622.13.7e-10287.27nifU-like protein 1, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_022991837.14.8e-10287.73nifU-like protein 1, chloroplastic [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G01940.13.0e-6661.74NFU domain protein 1[more]
AT5G49940.18.7e-2138.51NIFU-like protein 2[more]
AT4G25910.12.4e-1840.29NFU domain protein 3[more]
AT3G20970.18.4e-0837.65NFU domain protein 4[more]
AT1G51390.12.5e-0736.47NFU domain protein 5[more]
Match NameE-valueIdentityDescription
sp|Q93W77|NIFU1_ARATH5.5e-6561.74NifU-like protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU1 PE=1... [more]
sp|Q84LK7|NIFU1_ORYSJ1.6e-1939.11NifU-like protein 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=N... [more]
sp|Q93W20|NIFU2_ARATH1.6e-1938.51NifU-like protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU2 PE=1... [more]
sp|Q84RQ7|NIFU3_ARATH4.3e-1740.29NifU-like protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU3 PE=2... [more]
sp|O32119|YUTI_BACSU6.4e-1352.86Putative nitrogen fixation protein YutI OS=Bacillus subtilis (strain 168) OX=224... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0KB98|A0A0A0KB98_CUCSA1.4e-118100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G412870 PE=4 SV=1[more]
tr|A0A1S3BQX1|A0A1S3BQX1_CUCME6.6e-11697.27nifU-like protein 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492737 PE=4 ... [more]
tr|M5XUC8|M5XUC8_PRUPE7.2e-7872.15Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_3G007000 PE=4 SV=1[more]
tr|A0A140B480|A0A140B480_PRUPE7.2e-7872.15NFU domain protein 1 OS=Prunus persica var. persica OX=323852 GN=NFU1 PE=2 SV=1[more]
tr|A0A2I4DNY7|A0A2I4DNY7_9ROSI9.4e-7872.15nifU-like protein 1, chloroplastic OS=Juglans regia OX=51240 GN=LOC108982048 PE=... [more]
The following terms have been associated with this mRNA:
Vocabulary: Biological Process
TermDefinition
GO:0016226iron-sulfur cluster assembly
Vocabulary: Molecular Function
TermDefinition
GO:0051536iron-sulfur cluster binding
GO:0005506iron ion binding
Vocabulary: INTERPRO
TermDefinition
IPR001075NIF_FeS_clus_asmbl_NifU_C
IPR034904FSCA_dom_sf
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016226 iron-sulfur cluster assembly
cellular_component GO:0009507 chloroplast
molecular_function GO:0005506 iron ion binding
molecular_function GO:0051536 iron-sulfur cluster binding

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CsGy7G016780CsGy7G016780gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CsGy7G016780.1.CDS.1CsGy7G016780.1.CDS.1CDS
CsGy7G016780.1.CDS.2CsGy7G016780.1.CDS.2CDS
CsGy7G016780.1.CDS.3CsGy7G016780.1.CDS.3CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CsGy7G016780.1CsGy7G016780.1-proteinpolypeptide


Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR034904Fe-S cluster assembly domain superfamilyGENE3DG3DSA:3.30.300.130coord: 71..156
e-value: 3.8E-27
score: 95.9
IPR034904Fe-S cluster assembly domain superfamilyGENE3DG3DSA:3.30.300.130coord: 157..217
e-value: 2.2E-9
score: 39.3
IPR034904Fe-S cluster assembly domain superfamilySUPERFAMILYSSF117916Fe-S cluster assembly (FSCA) domain-likecoord: 77..149
IPR034904Fe-S cluster assembly domain superfamilySUPERFAMILYSSF117916Fe-S cluster assembly (FSCA) domain-likecoord: 155..217
IPR001075NIF system FeS cluster assembly, NifU, C-terminalPFAMPF01106NifUcoord: 161..216
e-value: 9.4E-5
score: 22.4
coord: 80..139
e-value: 7.6E-23
score: 80.4
IPR001075NIF system FeS cluster assembly, NifU, C-terminalPRODOMPD002830coord: 83..135
e-value: 9.0E-10
score: 151.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR11178:SF15NIFU-LIKE PROTEIN 1, CHLOROPLASTICcoord: 57..216
NoneNo IPR availablePANTHERPTHR11178IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATEDcoord: 57..216