CsGy3G007550.1 (mRNA) Cucumber (Gy14) v2

NameCsGy3G007550.1
TypemRNA
OrganismCucumis sativus (Cucumber (Gy14) v2)
DescriptionStAR-related lipid transfer protein 9, putative
LocationChr3 : 6527365 .. 6529161 (-)
Sequence length513
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGAGAACGGAGGAAGCGACGCTGGTAGCAACGCCCATTGGTGGTGGGCTTTCGCCGGTGCAGCGCAGTTAGGTTGGGGTATCGCCTCTTTCCGGCGAGGATTTATCGGTGATTCCAGCAACATGCCCCTCAAGGCCTTCGCCGTCGCTTCGCTTTTCGTCGGCGCTGCCGCCTCGTCTACAATTGCTTCCCTCAAGGCCTCTGGCATCCACAAGGTTCACCTCACTGCTTTTCTACTGTGTATTTATCAGTCGCATTTTTTCTTCTTCCCTCCCCCTCATCCTGGATTAGGTTAGAGGTTAATTGGACGACTTTGATCAGTCTGGTTTTGTACTCGTATCGGACTTTGGTGAATTAACTGAACAGATTAGTTTTGATTCGTCAGTATGCCGTGAATTTAGCTAATTTCCAGGAGGAATGGGATTTTGAGTTTTTGGCAGCTTAGTTAGCTTAATTTGTTAATGTTTGATTCGAACTATCAACCTTGTTGAAGTGAACAGTGAAATTGATCAGGGGATGAAAAAATACTTCAGTGGGTGTCAGTTTCTTTGGTCGCATTAGATTATTTGTTGCTGATGCCTGATTTTAGTCATGCTGTTTATCTTGGGCAGTTTGTTCGCTTGGCTTGATGTTGTGCTTAAATTCGAGAATCTTCTTAGTTGAATTTAGGTGTTTTGATTTTGAGTGGTTGATTCATGCCTATTTTTCATGTCGTTTTTTTCTGTTGGTATTATCCGCTTCTTTAAATTTAGAGGTTTAGGGTGTGGGATAAAACTAAAAGTTCTAACTTTCATAAACCTCATTTCAAAACTACTCATAGAATATAATATTTTAGAATGTTGAACAGAAAGTGTAATGATGGAGTTAAGTCTAAAAGAATGACATTCAAGCACAAACCTTTTGTCCAAAATTCTAATGTTTTTTCTACTTCAAGATAATTTTGAAACCTTTATGGAGCATTTTTTAATAAAGGACAAAGTTCAAGAGTGTTTTTTTCAAATAAAATAAAATATAGTCTAAATTCTGGTACACTTGGAAACCGCATACAAGTTGAGGCTTTGCCTCCTTCAAGTGGCAATCCTTATGGTAAAGGTTTGATGCTTTGGGTGGGAATTTAACCTTTCATTGTCTTTCCGTAGAAAGCGGTGTTGAACTCAATTCTGAAGCCTTCTGTCAGTTCAATGCACAAGTAACAGATGCTTTTTTCGGTTAATAAGAATAACTGTAGAGTATCTGTTGTTTGTTTAGGAGTATGAATGGTTTATTTGTTTGTTTTTAATATCATAGCTTCACTGTAATTGGTATGTGATAGGCAGAGGTAATTTGAACATATTAAGAGCGATGCTTTTTCACATAGGTGAAAGCCTCCATGATCAACAAGGTGGTAGGTAGCATTCATGGTTATGAGAGGCTTGTGATATAGAAATAGGATATCGATGACACTGTTATGAACTTAGAAGAACTTTGACGACCAGAAATGGTTTTCCTTTTCCCTTGTTGTTCTAATGGGTGAACAAACGAAGCCATTGTTATCAGTTCTTACAAAATCATGGGTTGGCGAAACGATTTCAATGGATGCAGAATTTCTTAAGAAGTGTTTTGATCTGGACGAAAGAAAAGGAGAACACGTCTGAAAAAGAAATTTACTAGATCTAGAAATGGGTGATGAAGTGAATGGTACGATTACTAATGTTTGGTTTTGGGGTGTACTGGAACAGGTGGAAGACGTGATGGAAGTAGGGGCCAGAATCAGAAGTGGATTGGGGATTCGTTCAAGGGCACAAGATAAATGA

mRNA sequence

ATGGAGGAGAACGGAGGAAGCGACGCTGGTAGCAACGCCCATTGGTGGTGGGCTTTCGCCGGTGCAGCGCAGTTAGGTTGGGGTATCGCCTCTTTCCGGCGAGGATTTATCGGTGATTCCAGCAACATGCCCCTCAAGGCCTTCGCCGTCGCTTCGCTTTTCGTCGGCGCTGCCGCCTCGTCTACAATTGCTTCCCTCAAGGCCTCTGGCATCCACAAGGTTCACCTCACTGCTTTTCTACTGTGTATTTATCAGTCGCATTTTTTCTTCTTCCCTCCCCCTCATCCTGGATTAGAAAGCGGTGTTGAACTCAATTCTGAAGCCTTCTGTCAGTTCAATGCACAACTTCACTGTAATTGGTATGTGATAGGCAGAGAAATGGGTGATGAAGTGAATGGTACGATTACTAATGTTTGGTTTTGGGGTGTACTGGAACAGGTGGAAGACGTGATGGAAGTAGGGGCCAGAATCAGAAGTGGATTGGGGATTCGTTCAAGGGCACAAGATAAATGA

Coding sequence (CDS)

ATGGAGGAGAACGGAGGAAGCGACGCTGGTAGCAACGCCCATTGGTGGTGGGCTTTCGCCGGTGCAGCGCAGTTAGGTTGGGGTATCGCCTCTTTCCGGCGAGGATTTATCGGTGATTCCAGCAACATGCCCCTCAAGGCCTTCGCCGTCGCTTCGCTTTTCGTCGGCGCTGCCGCCTCGTCTACAATTGCTTCCCTCAAGGCCTCTGGCATCCACAAGGTTCACCTCACTGCTTTTCTACTGTGTATTTATCAGTCGCATTTTTTCTTCTTCCCTCCCCCTCATCCTGGATTAGAAAGCGGTGTTGAACTCAATTCTGAAGCCTTCTGTCAGTTCAATGCACAACTTCACTGTAATTGGTATGTGATAGGCAGAGAAATGGGTGATGAAGTGAATGGTACGATTACTAATGTTTGGTTTTGGGGTGTACTGGAACAGGTGGAAGACGTGATGGAAGTAGGGGCCAGAATCAGAAGTGGATTGGGGATTCGTTCAAGGGCACAAGATAAATGA

Protein sequence

MEENGGSDAGSNAHWWWAFAGAAQLGWGIASFRRGFIGDSSNMPLKAFAVASLFVGAAASSTIASLKASGIHKVHLTAFLLCIYQSHFFFFPPPHPGLESGVELNSEAFCQFNAQLHCNWYVIGREMGDEVNGTITNVWFWGVLEQVEDVMEVGARIRSGLGIRSRAQDK
BLAST of CsGy3G007550.1 vs. NCBI nr
Match: XP_004133779.1 (PREDICTED: uncharacterized protein LOC101221895 [Cucumis sativus] >KGN56372.1 hypothetical protein Csa_3G118070 [Cucumis sativus])

HSP 1 Score: 157.5 bits (397), Expect = 3.9e-35
Identity = 97/170 (57.06%), Postives = 97/170 (57.06%), Query Frame = 0

Query: 1   MEENGGSDAGSNAHWWWAFAGAAQLGWGIASFRRGFIGDSSNMPLKAFAVASLFVGAAAS 60
           MEENGGSDAGSNAHWWWAFAGAAQLGWGIASFRRGFIGDSSNMPLKAFAVASLFVGAAAS
Sbjct: 1   MEENGGSDAGSNAHWWWAFAGAAQLGWGIASFRRGFIGDSSNMPLKAFAVASLFVGAAAS 60

Query: 61  STIASLKASGIHKVHLTAFLLCIYQSHFFFFPPPHPGLESGVELNSEAFCQFNAQLHCNW 120
           STIASLKASGIHK                                               
Sbjct: 61  STIASLKASGIHK----------------------------------------------- 97

Query: 121 YVIGREMGDEVNGTITNVWFWGVLEQVEDVMEVGARIRSGLGIRSRAQDK 171
                                     VEDVMEVGARIRSGLGIRSRAQDK
Sbjct: 121 --------------------------VEDVMEVGARIRSGLGIRSRAQDK 97

BLAST of CsGy3G007550.1 vs. NCBI nr
Match: XP_022974845.1 (uncharacterized protein LOC111473617 isoform X1 [Cucurbita maxima] >XP_022975308.1 uncharacterized protein LOC111474468 isoform X1 [Cucurbita maxima])

HSP 1 Score: 127.9 bits (320), Expect = 3.3e-26
Identity = 81/170 (47.65%), Postives = 86/170 (50.59%), Query Frame = 0

Query: 1   MEENGGSDAGSNAHWWWAFAGAAQLGWGIASFRRGFIGDSSNMPLKAFAVASLFVGAAAS 60
           M ENGGSD GSNAHWWWA AGA QLGWGIASFRRG IGDSS MP KAFAVASLFVGAA+S
Sbjct: 28  MVENGGSDGGSNAHWWWALAGATQLGWGIASFRRGVIGDSSLMPFKAFAVASLFVGAASS 87

Query: 61  STIASLKASGIHKVHLTAFLLCIYQSHFFFFPPPHPGLESGVELNSEAFCQFNAQLHCNW 120
           S IASLKA+GIHK                                               
Sbjct: 88  SAIASLKAAGIHK----------------------------------------------- 124

Query: 121 YVIGREMGDEVNGTITNVWFWGVLEQVEDVMEVGARIRSGLGIRSRAQDK 171
                                     VEDVMEVGA IR+GLGIR R +D+
Sbjct: 148 --------------------------VEDVMEVGANIRNGLGIRPRTRDE 124

BLAST of CsGy3G007550.1 vs. NCBI nr
Match: XP_022941577.1 (uncharacterized protein LOC111446882 isoform X2 [Cucurbita moschata])

HSP 1 Score: 127.5 bits (319), Expect = 4.4e-26
Identity = 81/170 (47.65%), Postives = 85/170 (50.00%), Query Frame = 0

Query: 1   MEENGGSDAGSNAHWWWAFAGAAQLGWGIASFRRGFIGDSSNMPLKAFAVASLFVGAAAS 60
           M ENGGSD GSNAHWWWA AGA Q GWGIASFRRG IGDSS MP KAFAVASLFVGAAAS
Sbjct: 28  MVENGGSDGGSNAHWWWALAGATQFGWGIASFRRGVIGDSSLMPFKAFAVASLFVGAAAS 87

Query: 61  STIASLKASGIHKVHLTAFLLCIYQSHFFFFPPPHPGLESGVELNSEAFCQFNAQLHCNW 120
           S IASLKA+GIHK                                               
Sbjct: 88  SAIASLKAAGIHK----------------------------------------------- 124

Query: 121 YVIGREMGDEVNGTITNVWFWGVLEQVEDVMEVGARIRSGLGIRSRAQDK 171
                                     VEDVMEVGA IR+GLGIR R +D+
Sbjct: 148 --------------------------VEDVMEVGANIRTGLGIRPRTRDE 124

BLAST of CsGy3G007550.1 vs. NCBI nr
Match: XP_023540026.1 (uncharacterized protein LOC111800524 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 127.1 bits (318), Expect = 5.7e-26
Identity = 80/170 (47.06%), Postives = 85/170 (50.00%), Query Frame = 0

Query: 1   MEENGGSDAGSNAHWWWAFAGAAQLGWGIASFRRGFIGDSSNMPLKAFAVASLFVGAAAS 60
           M ENGGSD GSNAHWWWA AGA Q GWGIASFRRG IGDSS MP KAFAVASLFVGAAAS
Sbjct: 6   MVENGGSDGGSNAHWWWALAGATQFGWGIASFRRGVIGDSSLMPFKAFAVASLFVGAAAS 65

Query: 61  STIASLKASGIHKVHLTAFLLCIYQSHFFFFPPPHPGLESGVELNSEAFCQFNAQLHCNW 120
           S IASLKA+GIHK                                               
Sbjct: 66  SAIASLKAAGIHK----------------------------------------------- 102

Query: 121 YVIGREMGDEVNGTITNVWFWGVLEQVEDVMEVGARIRSGLGIRSRAQDK 171
                                     VEDVMEVGA IR+GLG+R R +D+
Sbjct: 126 --------------------------VEDVMEVGANIRTGLGVRPRTRDE 102

BLAST of CsGy3G007550.1 vs. NCBI nr
Match: XP_022145350.1 (uncharacterized protein LOC111014824 [Momordica charantia])

HSP 1 Score: 126.3 bits (316), Expect = 9.7e-26
Identity = 81/170 (47.65%), Postives = 87/170 (51.18%), Query Frame = 0

Query: 1   MEENGGSDAGSNAHWWWAFAGAAQLGWGIASFRRGFIGDSSNMPLKAFAVASLFVGAAAS 60
           ME+NGG D GSNAHWWWA AGA Q GWGIASFRRGFIG+SS MP KAFAVASLFVGAAAS
Sbjct: 1   MEDNGG-DGGSNAHWWWALAGATQFGWGIASFRRGFIGNSSLMPFKAFAVASLFVGAAAS 60

Query: 61  STIASLKASGIHKVHLTAFLLCIYQSHFFFFPPPHPGLESGVELNSEAFCQFNAQLHCNW 120
           S IASLKASGIHK                                               
Sbjct: 61  SAIASLKASGIHK----------------------------------------------- 96

Query: 121 YVIGREMGDEVNGTITNVWFWGVLEQVEDVMEVGARIRSGLGIRSRAQDK 171
                                     VEDVME+GA IRSGLG+R RA+D+
Sbjct: 121 --------------------------VEDVMEIGASIRSGLGVRPRARDE 96

BLAST of CsGy3G007550.1 vs. TAIR10
Match: AT3G60480.1 (unknown protein)

HSP 1 Score: 81.3 bits (199), Expect = 6.5e-16
Identity = 41/79 (51.90%), Postives = 54/79 (68.35%), Query Frame = 0

Query: 2  EENGGSDAGSNA------HWWWAFAGAAQLGWGIASFRRGFIGDSSNMPLKAFAVASLFV 61
          E+N G+ AG ++      HWWWA    AQ+ WGI   RRG+ GD   MPLKAF VASLFV
Sbjct: 3  EQNAGTGAGESSLLDGSGHWWWALGSGAQIMWGIRLIRRGYAGDVRLMPLKAFGVASLFV 62

Query: 62 GAAASSTIASLKASGIHKV 75
          G+ A+S++A ++A+GIH V
Sbjct: 63 GSLATSSVALVRATGIHTV 81

BLAST of CsGy3G007550.1 vs. TrEMBL
Match: tr|A0A0A0L700|A0A0A0L700_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G118070 PE=4 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 2.6e-35
Identity = 97/170 (57.06%), Postives = 97/170 (57.06%), Query Frame = 0

Query: 1   MEENGGSDAGSNAHWWWAFAGAAQLGWGIASFRRGFIGDSSNMPLKAFAVASLFVGAAAS 60
           MEENGGSDAGSNAHWWWAFAGAAQLGWGIASFRRGFIGDSSNMPLKAFAVASLFVGAAAS
Sbjct: 1   MEENGGSDAGSNAHWWWAFAGAAQLGWGIASFRRGFIGDSSNMPLKAFAVASLFVGAAAS 60

Query: 61  STIASLKASGIHKVHLTAFLLCIYQSHFFFFPPPHPGLESGVELNSEAFCQFNAQLHCNW 120
           STIASLKASGIHK                                               
Sbjct: 61  STIASLKASGIHK----------------------------------------------- 97

Query: 121 YVIGREMGDEVNGTITNVWFWGVLEQVEDVMEVGARIRSGLGIRSRAQDK 171
                                     VEDVMEVGARIRSGLGIRSRAQDK
Sbjct: 121 --------------------------VEDVMEVGARIRSGLGIRSRAQDK 97

BLAST of CsGy3G007550.1 vs. TrEMBL
Match: tr|A0A1S3AUW2|A0A1S3AUW2_CUCME (uncharacterized protein LOC103483131 OS=Cucumis melo OX=3656 GN=LOC103483131 PE=4 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 1.3e-21
Identity = 76/153 (49.67%), Postives = 77/153 (50.33%), Query Frame = 0

Query: 18  AFAGAAQLGWGIASFRRGFIGDSSNMPLKAFAVASLFVGAAASSTIASLKASGIHKVHLT 77
           AFAGA QLGWGIASFRRGF+GDSS MPLKAFAVASLFVGAAASS IASLKASGIHK    
Sbjct: 46  AFAGAGQLGWGIASFRRGFVGDSSTMPLKAFAVASLFVGAAASSAIASLKASGIHK---- 105

Query: 78  AFLLCIYQSHFFFFPPPHPGLESGVELNSEAFCQFNAQLHCNWYVIGREMGDEVNGTITN 137
                                                                       
Sbjct: 106 ------------------------------------------------------------ 125

Query: 138 VWFWGVLEQVEDVMEVGARIRSGLGIRSRAQDK 171
                    VEDVMEVGARIRSGLGIRSRAQDK
Sbjct: 166 ---------VEDVMEVGARIRSGLGIRSRAQDK 125

BLAST of CsGy3G007550.1 vs. TrEMBL
Match: tr|F6GV86|F6GV86_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_15s0021g02030 PE=4 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 3.8e-18
Identity = 51/74 (68.92%), Postives = 58/74 (78.38%), Query Frame = 0

Query: 1  MEENGGSDAGSNAHWWWAFAGAAQLGWGIASFRRGFIGDSSNMPLKAFAVASLFVGAAAS 60
          MEEN G  +G  +HWWWA A AAQLGWG+ S+RRG +GDSS MP+KAF VASLFVGAAAS
Sbjct: 1  MEEN-GHGSGDGSHWWWALASAAQLGWGVFSYRRGGVGDSSLMPVKAFGVASLFVGAAAS 60

Query: 61 STIASLKASGIHKV 75
          +    L ASGIHKV
Sbjct: 61 AAFGVLHASGIHKV 73

BLAST of CsGy3G007550.1 vs. TrEMBL
Match: tr|A0A2I4HDZ6|A0A2I4HDZ6_9ROSI (uncharacterized protein LOC109016350 OS=Juglans regia OX=51240 GN=LOC109016350 PE=4 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 4.6e-16
Identity = 64/169 (37.87%), Postives = 77/169 (45.56%), Query Frame = 0

Query: 2   EENGGSDAGSNAHWWWAFAGAAQLGWGIASFRRGFIGDSSNMPLKAFAVASLFVGAAASS 61
           E+ GG   G   HWWWA A  AQLG G++S+RRG+ GDS  MPLKAF VASLFVGAAAS+
Sbjct: 5   EKEGGRTYG---HWWWALASGAQLGLGVSSYRRGYAGDSRLMPLKAFVVASLFVGAAAST 64

Query: 62  TIASLKASGIHKVHLTAFLLCIYQSHFFFFPPPHPGLESGVELNSEAFCQFNAQLHCNWY 121
            ++ LKASGIHK                                                
Sbjct: 65  AVSVLKASGIHK------------------------------------------------ 97

Query: 122 VIGREMGDEVNGTITNVWFWGVLEQVEDVMEVGARIRSGLGIRSRAQDK 171
                                    VED+MEVGA IR+GLG+R RA+D+
Sbjct: 125 -------------------------VEDLMEVGANIRAGLGVRPRARDE 97

BLAST of CsGy3G007550.1 vs. TrEMBL
Match: tr|A0A2P5YGW3|A0A2P5YGW3_GOSBA (Uncharacterized protein OS=Gossypium barbadense OX=3634 GN=GOBAR_AA05737 PE=4 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 7.9e-16
Identity = 46/74 (62.16%), Postives = 60/74 (81.08%), Query Frame = 0

Query: 3  ENGGSDA--GSNAHWWWAFAGAAQLGWGIASFRRGFIGDSSNMPLKAFAVASLFVGAAAS 62
          ENGG +     N+HW+WA A A+QLGW ++S+R+G+ GD   MPLKAFAVASLF+GA+AS
Sbjct: 5  ENGGDNGKIHINSHWYWAIASASQLGWAVSSYRKGYTGDHRFMPLKAFAVASLFLGASAS 64

Query: 63 STIASLKASGIHKV 75
          +++A LKASGIHKV
Sbjct: 65 ASVAFLKASGIHKV 78

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004133779.13.9e-3557.06PREDICTED: uncharacterized protein LOC101221895 [Cucumis sativus] >KGN56372.1 hy... [more]
XP_022974845.13.3e-2647.65uncharacterized protein LOC111473617 isoform X1 [Cucurbita maxima] >XP_022975308... [more]
XP_022941577.14.4e-2647.65uncharacterized protein LOC111446882 isoform X2 [Cucurbita moschata][more]
XP_023540026.15.7e-2647.06uncharacterized protein LOC111800524 isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022145350.19.7e-2647.65uncharacterized protein LOC111014824 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT3G60480.16.5e-1651.90unknown protein[more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
tr|A0A0A0L700|A0A0A0L700_CUCSA2.6e-3557.06Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G118070 PE=4 SV=1[more]
tr|A0A1S3AUW2|A0A1S3AUW2_CUCME1.3e-2149.67uncharacterized protein LOC103483131 OS=Cucumis melo OX=3656 GN=LOC103483131 PE=... [more]
tr|F6GV86|F6GV86_VITVI3.8e-1868.92Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_15s0021g02030 PE=4 SV=... [more]
tr|A0A2I4HDZ6|A0A2I4HDZ6_9ROSI4.6e-1637.87uncharacterized protein LOC109016350 OS=Juglans regia OX=51240 GN=LOC109016350 P... [more]
tr|A0A2P5YGW3|A0A2P5YGW3_GOSBA7.9e-1662.16Uncharacterized protein OS=Gossypium barbadense OX=3634 GN=GOBAR_AA05737 PE=4 SV... [more]
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CsGy3G007550CsGy3G007550gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CsGy3G007550.1CsGy3G007550.1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CsGy3G007550.1.CDS.4CsGy3G007550.1.CDS.4CDS
CsGy3G007550.1.CDS.3CsGy3G007550.1.CDS.3CDS
CsGy3G007550.1.CDS.2CsGy3G007550.1.CDS.2CDS
CsGy3G007550.1.CDS.1CsGy3G007550.1.CDS.1CDS


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CsGy3G007550.1.exon.4CsGy3G007550.1.exon.4exon
CsGy3G007550.1.exon.3CsGy3G007550.1.exon.3exon
CsGy3G007550.1.exon.2CsGy3G007550.1.exon.2exon
CsGy3G007550.1.exon.1CsGy3G007550.1.exon.1exon


Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR37744FAMILY NOT NAMEDcoord: 1..75