Cp4.1LG18g00940.1 (mRNA) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG18g00940.1
Type	mRNA
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	Kinase family protein
Location	Cp4.1LG18 : 2411259 .. 2414036 (+)
Sequence length	1008

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCTGCTTTCCATGTTTCTCGTCTCAGAAGAGCAAGAAAGGAATCGATAAGAACGGCAATGACAATGCTTCCTCATCCTCCTCCGGCCACCGCACTCCTCCGCCCCCCAAATCCCCTGGTCTGTTCTTCTCCATTCATCCCCCCCACTAAGTCATATCATATTGATAATTAATATAATGTATTTTGATGTTATAAGCTTATATCATCATTTTGATATCTTAATATTTTTGAAGTATGAGATTTAAAAAAAATAACCTAAGGGTAATATTTATTTAGCCAAATACAAAACTGAAAAATGAAATGCCAGCAATATCGAAAAAAGAATAATTTAAGAGCATGAAATTAAATAATATCTTTTAGTATGAATGAAGAGAGTATGGGTTAAATTTTGAAGAATGGGACTTAAATTAAACAGAGACTACCACACCGAAGCCTCCTGAGGAAGTCAAGGACAAACCACAAGAAGGTGAAAAAATAGATGCCCAAAGCTTCACTTTCCGGGAGCTAGCCACTGCTACAAAGAATTTCCGTCAGGAATGTCTTTTGGGCGAAGGTGGATTTGGGAAAGTCTTTAAGGCTACCCTACAAAGCGGCCAGGTAACTAACCAAAATTTCCGAGCATAGATTAAGGGGACGTTTAGATTGCCTTTCTACGTGCTTAAAGAAGTGTTTCTAAGTGGGGAAAAATATGGCTTTAAGCAATTAGAAAGTCGATCCAAACAAGTCCGGGATATTAACTCTCCTCGTTCGTAATATTCTAAGAAGGAATGTTCATAGAGATTGTTTGTTTGGGTTGTGTGTTTTTATTTAAGGTGGTGGCTGTGAAGCAGCTGGATAGAAATGGGCTACAAGGAAACAAGGATTTTCTTGGTGAGGTCAACGAATTGAGCCTTCTAAGCCATCCAAATCTTGTGAAGTTCAATGGATATTGTGCAGATGGAGATCAAAGGATTTTGGTCTATGAATATATGCCAGGAGGTTCTTTGGGAGATCGTCTCTTTGGTATGTACTCCGAGTACTATAAATTCAATTCTCTAAACTTAGGAACTTGTTACATTATTGAGATATCATGTGGTTTGATTATAAGGTAAATGTTGATGGATTGATTTAGCATCATGATGTGTAAAATAATGTAGTTCATGCAAAATGCATACCATTTTTTATCTGGCTGCATCGTCGACCTCAAGATCCAGTAATTTGTTTGCCAAATAATATCCAATCAGCTACCTATTAAACAAACATGTTTCATTAAACTATGTTTCAATATTGGCAGACATAAAAGAGGATCAAAAGCCATTGGATTGGTTTGTTCGTATAAAGATAGCATCAGGAGTTGCAAAAGGATTGGAATACCTGCATGATCAAGCAGATCCTCCCATAATATTTCGTGATTTGAAGTCATCAAACATCTTGCTAGATGAGGATTTTAATCCAAAGCTTTCTGATTTCGGGCTCGCAAAGCTCGGCTCTGGCGGTGATAAGTCACCGTTGCCATCGAGGGTCATGGGCACCTATGGTTACTCTGCTCCTGAATACACAAGTGGTGGACAACTCACTTCCAAGTCTGATATTTACAGCTTTGGAGTTGTGATGCTTGAACTCATTACCGGAAGAAAAGCCGTTGACACTACAAAGCCACATAACGAGCAGAATCTTGTTACTTGGGTATGTTTTTCTTTTTAATTTGTGTTTGGATTTAAATTTGGTGGATCCAACTTATAAAAACATGCTTAATGCAAAGAATCAGGCATAATGTTTGAAACTATAAAACTAAAAATTCAATCATTAACAAATAAACCCAAATTTAGGAAACTTAGTTGAGTTTCTAACTTTCAGATGAAGGATCATTGAAAACTATATGGGTTATATGAACAGTTTGAAATTTCTAGTGAGATTCACATGCAGAAATAAACAGCTAAAAAAATGAACTCACAATATAGAGGCCTTTTCCTTCTGCCTTGCCCAAGAATTTATCAATTGGACAATGATTTCAGTACTCCTTTAAATTATGTGAGCTTTGAAGCCATGGCTGTGTAACAGGCACAACCATTTTTCCGAGATCCAAAGAGATTTCCAGACCTGGCCGATCCACTCCTCGGGCGATTGTTCCCGGAGAAAGATCTGAATCAGGCCGTTGCCGTCACCGCCATGTGTCTGCAAGAGGAAGCAGAAGTCCGTCCTCTGATCGCCGACGTCATGACGGCTCTCAGTTTTCTTTCCACAGTTCCAGACGAGAATCTCACTCCACTCCCTTATCCACCGGAACCCGAGCCTGAGGATGAGGAGGAGGATGAGGAGGATGCAGGCGACGATTACTCTTCAGAATCTTCGGATTCCGATACCGAATCTCGAAAAATAGGAAAAGGAGACAAAGACGGCGCATCTGCAAATTTCACAAGCGCAAGAAACCACGACTCAGATACCTCAGATGATGAAGATGAACAAAAAAACAATTCGAGCAAGAAGAAACCTAAAAACAAAGACTCTGAACAAAAACGAGTGGTATTCAAAGACGACGTACAGCCAAATCACAGCAGAAAAAACAGCTCCTCATCGAGCGATGGAGGCTCACCTAGAAGCAGCAACGCATCAGAAAATAGAAGAATCGACAGCAGAGATTCATCTTTCAAAATGCAAAAAGAAGCTTCGGATATTGTAAATAGTACCGATTCTACATTAGAGCATAAGAGCAGTAGGGAATCGAAATCTGGCGAAGGTCAAAACAACTCCAGAAGCGATTCCGACGGCGTCTCTGAACATGACGGCAAAGATTGA

mRNA sequence

ATGAGCTGCTTTCCATGTTTCTCGTCTCAGAAGAGCAAGAAAGGAATCGATAAGAACGGCAATGACAATGCTTCCTCATCCTCCTCCGGCCACCGCACTCCTCCGCCCCCCAAATCCCCTGAGACTACCACACCGAAGCCTCCTGAGGAAGTCAAGGACAAACCACAAGAAGGTGAAAAAATAGATGCCCAAAGCTTCACTTTCCGGGAGCTAGCCACTGCTACAAAGAATTTCCGTCAGGAATGTCTTTTGGGCGAAGGTGGATTTGGGAAAGCACAACCATTTTTCCGAGATCCAAAGAGATTTCCAGACCTGGCCGATCCACTCCTCGGGCGATTGTTCCCGGAGAAAGATCTGAATCAGGCCGTTGCCGTCACCGCCATGTGTCTGCAAGAGGAAGCAGAAGTCCGTCCTCTGATCGCCGACGTCATGACGGCTCTCAGTTTTCTTTCCACAGTTCCAGACGAGAATCTCACTCCACTCCCTTATCCACCGGAACCCGAGCCTGAGGATGAGGAGGAGGATGAGGAGGATGCAGGCGACGATTACTCTTCAGAATCTTCGGATTCCGATACCGAATCTCGAAAAATAGGAAAAGGAGACAAAGACGGCGCATCTGCAAATTTCACAAGCGCAAGAAACCACGACTCAGATACCTCAGATGATGAAGATGAACAAAAAAACAATTCGAGCAAGAAGAAACCTAAAAACAAAGACTCTGAACAAAAACGAGTGGTATTCAAAGACGACGTACAGCCAAATCACAGCAGAAAAAACAGCTCCTCATCGAGCGATGGAGGCTCACCTAGAAGCAGCAACGCATCAGAAAATAGAAGAATCGACAGCAGAGATTCATCTTTCAAAATGCAAAAAGAAGCTTCGGATATTGTAAATAGTACCGATTCTACATTAGAGCATAAGAGCAGTAGGGAATCGAAATCTGGCGAAGGTCAAAACAACTCCAGAAGCGATTCCGACGGCGTCTCTGAACATGACGGCAAAGATTGA

Coding sequence (CDS)

ATGAGCTGCTTTCCATGTTTCTCGTCTCAGAAGAGCAAGAAAGGAATCGATAAGAACGGCAATGACAATGCTTCCTCATCCTCCTCCGGCCACCGCACTCCTCCGCCCCCCAAATCCCCTGAGACTACCACACCGAAGCCTCCTGAGGAAGTCAAGGACAAACCACAAGAAGGTGAAAAAATAGATGCCCAAAGCTTCACTTTCCGGGAGCTAGCCACTGCTACAAAGAATTTCCGTCAGGAATGTCTTTTGGGCGAAGGTGGATTTGGGAAAGCACAACCATTTTTCCGAGATCCAAAGAGATTTCCAGACCTGGCCGATCCACTCCTCGGGCGATTGTTCCCGGAGAAAGATCTGAATCAGGCCGTTGCCGTCACCGCCATGTGTCTGCAAGAGGAAGCAGAAGTCCGTCCTCTGATCGCCGACGTCATGACGGCTCTCAGTTTTCTTTCCACAGTTCCAGACGAGAATCTCACTCCACTCCCTTATCCACCGGAACCCGAGCCTGAGGATGAGGAGGAGGATGAGGAGGATGCAGGCGACGATTACTCTTCAGAATCTTCGGATTCCGATACCGAATCTCGAAAAATAGGAAAAGGAGACAAAGACGGCGCATCTGCAAATTTCACAAGCGCAAGAAACCACGACTCAGATACCTCAGATGATGAAGATGAACAAAAAAACAATTCGAGCAAGAAGAAACCTAAAAACAAAGACTCTGAACAAAAACGAGTGGTATTCAAAGACGACGTACAGCCAAATCACAGCAGAAAAAACAGCTCCTCATCGAGCGATGGAGGCTCACCTAGAAGCAGCAACGCATCAGAAAATAGAAGAATCGACAGCAGAGATTCATCTTTCAAAATGCAAAAAGAAGCTTCGGATATTGTAAATAGTACCGATTCTACATTAGAGCATAAGAGCAGTAGGGAATCGAAATCTGGCGAAGGTCAAAACAACTCCAGAAGCGATTCCGACGGCGTCTCTGAACATGACGGCAAAGATTGA

Protein sequence

MSCFPCFSSQKSKKGIDKNGNDNASSSSSGHRTPPPPKSPETTTPKPPEEVKDKPQEGEKIDAQSFTFRELATATKNFRQECLLGEGGFGKAQPFFRDPKRFPDLADPLLGRLFPEKDLNQAVAVTAMCLQEEAEVRPLIADVMTALSFLSTVPDENLTPLPYPPEPEPEDEEEDEEDAGDDYSSESSDSDTESRKIGKGDKDGASANFTSARNHDSDTSDDEDEQKNNSSKKKPKNKDSEQKRVVFKDDVQPNHSRKNSSSSSDGGSPRSSNASENRRIDSRDSSFKMQKEASDIVNSTDSTLEHKSSRESKSGEGQNNSRSDSDGVSEHDGKD

Homology

[Close]

BLAST of Cp4.1LG18g00940.1 vs. Swiss-Prot
Match: Y3707_ARATH (Serine/threonine-protein kinase At3g07070 OS=Arabidopsis thaliana GN=At3g07070 PE=2 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.8e-19
Identity = 52/86 (60.47%), Postives = 64/86 (74.42%), Query Frame = 1


Query: 92  AQPFFRDPKRFPDLADPLLGRLFPEKDLNQAVAVTAMCLQEEAEVRPLIADVMTALSFLS 151
           AQP F++P RFP+LADP L  +FPEK LNQAVAV AMCLQEEA VRPL++DV+TAL FL 
Sbjct: 298 AQPVFKEPSRFPELADPSLEGVFPEKALNQAVAVAAMCLQEEATVRPLMSDVVTALGFLG 357

Query: 152 TVPDENLTPLPYPPEPEPEDEEEDEE 178
           T PD +++   Y   P+P DE   E+
Sbjct: 358 TAPDGSISVPHYDDPPQPSDETSVED 383

[Close]

BLAST of Cp4.1LG18g00940.1 vs. Swiss-Prot
Match: LIPG1_ARATH (Receptor-like kinase LIP1 OS=Arabidopsis thaliana GN=LIP1 PE=1 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 3.9e-14
Identity = 94/258 (36.43%), Postives = 133/258 (51.55%), Query Frame = 1


Query: 92  AQPFFRDPKRFPDLADPLLGRLFPEKDLNQAVAVTAMCLQEEAEVRPLIADVMTALSFLS 151
           AQP F+DPKR+PD+ADPLL + F E+ LNQAVA+T+MCLQEE   RPLI+DVM ALSFLS
Sbjct: 294 AQPIFKDPKRYPDMADPLLRKNFSERGLNQAVAITSMCLQEEPTARPLISDVMVALSFLS 353

Query: 152 TVPDENLTPLPYPPEPEPEDEEE----------------DEEDAGDDYSSESSDSDTESR 211
              ++ + P   P E   +                      +D G+  SS S   D E  
Sbjct: 354 MSTEDGI-PATVPMESFRDKSMSIALSRHGSCSVTPFCISRKDVGNKSSSSSDSEDEEEE 413

Query: 212 KIGKGDKDGASANFTSARNHDSDTSDDEDEQKNNSSKKKPKNKDSEQKRVVFKDDVQPNH 271
           K  K +K+  S +    R    +T+ D D++ +++S+K     D E      ++  Q   
Sbjct: 414 KEQKAEKEEESTS--KKRQEQEETATDSDDESDSNSEK-----DQE------EEQSQLEK 473

Query: 272 SRKNSSSSSDGGSPRSSNASENRRIDSRDSSFKMQKEASDIVNSTDSTLEHKSSRES-KS 331
           +R++SSSSSD G       SE R ID  +++ +  K +    +S +   E  SS+ S KS
Sbjct: 474 ARESSSSSSDSG-------SERRSIDETNATAQSLKISYSNYSSEEEDNEKLSSKSSCKS 528

Query: 332 GEGQNNSRSDSDGVSEHD 333
            E    SR DS    +HD
Sbjct: 534 NEESTFSRYDSG--RDHD 528

[Close]

BLAST of Cp4.1LG18g00940.1 vs. Swiss-Prot
Match: CDL1_ARATH (Serine/threonine-protein kinase CDL1 OS=Arabidopsis thaliana GN=CDL1 PE=1 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 4.7e-12
Identity = 44/108 (40.74%), Postives = 62/108 (57.41%), Query Frame = 1


Query: 60  KIDAQSF--TFRELATATKNFRQECLLGEGGF-GKAQPFFRDPKRFPDLADPLLGRLFPE 119
           K D  SF     E+ T  K        GE      A+P F+D ++F  +ADP+L   +P 
Sbjct: 257 KSDVYSFGVVLLEIITGRKAIDSSRSTGEQNLVAWARPLFKDRRKFSQMADPMLQGQYPP 316

Query: 120 KDLNQAVAVTAMCLQEEAEVRPLIADVMTALSFLSTVPDENLTPLPYP 165
           + L QA+AV AMC+QE+  +RPLIADV+TALS+L++   +   PL  P
Sbjct: 317 RGLYQALAVAAMCVQEQPNLRPLIADVVTALSYLAS---QKFDPLAQP 361

[Close]

BLAST of Cp4.1LG18g00940.1 vs. Swiss-Prot
Match: LIPG2_ARATH (Receptor-like kinase LIP2 OS=Arabidopsis thaliana GN=LIP2 PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 3.1e-11
Identity = 45/92 (48.91%), Postives = 53/92 (57.61%), Query Frame = 1


Query: 1  MSCFPCFSSQKSKKGIDKNG-NDNASSSSSGHRTPPPPKSPETTTPKPPEEVKDKPQEGE 60
          M CFPCFSS K+KK    N  NDN             PK  +    +  EE+    ++ E
Sbjct: 1  MHCFPCFSSPKNKKSSTTNETNDNNE-----------PKPDDRRRAEETEEI----EQSE 60

Query: 61 KIDAQSFTFRELATATKNFRQECLLGEGGFGK 92
              + FTFRELATATKNFRQECLLGEGGFG+
Sbjct: 61 GTSLKIFTFRELATATKNFRQECLLGEGGFGR 77

[Close]

BLAST of Cp4.1LG18g00940.1 vs. Swiss-Prot
Match: RLCK7_ARATH (Probable serine/threonine-protein kinase RLCKVII OS=Arabidopsis thaliana GN=At1g07870 PE=2 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 2.4e-08
Identity = 36/89 (40.45%), Postives = 56/89 (62.92%), Query Frame = 1


Query: 90  GKAQPFFRDPKRFPDLADPLLGRLFPEKDLNQAVAVTAMCLQEEAEVRPLIADVMTALSF 149
           G A+P F+D + FP + DPLL   +P + L QA+A++AMC+QE+  +RP+++DV+ AL+F
Sbjct: 320 GWARPLFKDRRNFPKMVDPLLQGQYPVRGLYQALAISAMCVQEQPTMRPVVSDVVLALNF 379

Query: 150 L-STVPDENLTPLPYPPEPEPEDEEEDEE 178
           L S+  D N         P    + +DEE
Sbjct: 380 LASSKYDPNSPSSSSGKNPSFHRDRDDEE 408

[Close]

BLAST of Cp4.1LG18g00940.1 vs. TrEMBL
Match: A0A0A0LY64_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G662820 PE=4 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 1.3e-77
Identity = 221/341 (64.81%), Postives = 242/341 (70.97%), Query Frame = 1


Query: 24  ASSSSSGHRTPPPPKSPETTTPKPPEEVKDKPQEGEKIDAQSF--------TFRELATAT 83
           A     G ++P P +   T     PE  +   Q   K D  SF        T R+    T
Sbjct: 231 AKLGPGGDKSPLPSRVMGTYGYSAPEYTRGG-QLTSKSDIYSFGVVMLELITGRKAIDTT 290

Query: 84  KNFRQECLLGEGGFGKAQPFFRDPKRFPDLADPLLGRLFPEKDLNQAVAVTAMCLQEEAE 143
           K   ++ L+       AQPFFRDPKRFPDLADPLLGRLFPEKDLNQAVAV AMCLQEEAE
Sbjct: 291 KPNNEQNLVTW-----AQPFFRDPKRFPDLADPLLGRLFPEKDLNQAVAVAAMCLQEEAE 350

Query: 144 VRPLIADVMTALSFLSTVPDENLTPLPYPPEPEPEDEEEDEEDAGDDYSSESSDSDTESR 203
           VRPLI DVMTALSFLSTVPDENL PLPYPPEPEPE E E EE+  DD SSESSDSDTESR
Sbjct: 351 VRPLIGDVMTALSFLSTVPDENLRPLPYPPEPEPEPEPE-EENRDDDSSSESSDSDTESR 410

Query: 204 KIGKGDKDGASANFTSARNHDSDT-SDDEDE-QKNN--SSKKKPKNKDSEQKRVVFKDDV 263
           KIGK DKD ASANFTSARNH++DT SDDEDE +K N  SSKKKPKNK+SEQKRVVFKDD+
Sbjct: 411 KIGK-DKDCASANFTSARNHETDTSSDDEDEGEKENIKSSKKKPKNKESEQKRVVFKDDI 470

Query: 264 Q---PNHSRKNSSSSSDG-GSPRSSNASENRRIDS---------RDSSFKMQKEASDIVN 323
           Q   PNH+RKNSSSSSDG  SPRSSNASENRRIDS          DSSFK+Q E SD+VN
Sbjct: 471 QPPAPNHTRKNSSSSSDGSSSPRSSNASENRRIDSLESIRDSEGGDSSFKLQDEPSDVVN 530

Query: 324 STDSTLEHKSSRESKSG----EGQNNSRSDSDGVSEHDGKD 336
           S DSTLEH SSRES +G    E  N   SDS+  SEH G++
Sbjct: 531 SNDSTLEHISSRESHTGDEEYEAHNEYSSDSEEGSEHGGEE 563

[Close]

BLAST of Cp4.1LG18g00940.1 vs. TrEMBL
Match: W9SA67_9ROSA (Serine/threonine-protein kinase OS=Morus notabilis GN=L484_006459 PE=3 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 1.3e-19
Identity = 89/234 (38.03%), Postives = 130/234 (55.56%), Query Frame = 1


Query: 92  AQPFFRDPKRFPDLADPLLGRLFPEKDLNQAVAVTAMCLQEEAEVRPLIADVMTALSFLS 151
           AQPFFRDPK+FPDLADPLL R FPEKDLNQ VA+ AMCLQ+EA  RPLI+D++T LSFLS
Sbjct: 283 AQPFFRDPKKFPDLADPLLNRQFPEKDLNQVVAIAAMCLQDEASARPLISDIVTTLSFLS 342

Query: 152 TVPDENLTPLPYPPEPEPEDE--------EEDEEDAGDDYSSESSDS-DTESRKIGKGDK 211
           T P E +   P P + +  D         ++ E D  DD   ESS+S ++ES        
Sbjct: 343 TTPSECIQTAPTPQDGKDSDSKSSKHENAKDQESDCSDDEDGESSESQESESSDYEDVKS 402

Query: 212 DGASANFTSARNHDSDTSDDEDEQKNNSSKKKPKNKDSEQKRVVFKDDVQPNHSRKNSSS 271
              + ++ S+R      S+D+D +  +SS+K     D           V  + S  +++S
Sbjct: 403 RDEAKDWQSSRKSSMAGSEDDDNESISSSQKSSSGSDD--------GSVSSSQSGSSTNS 462

Query: 272 SSDGGSPRSSNASENRRIDSRDSSFKMQKEASDIVN-STDSTLEHKSSRESKSG 316
            +   S R+S+   NR + + +S    +K ++  V+   D  L   S++ES++G
Sbjct: 463 KASSVSSRNSSIGSNRPVSNFNSQRSSRKSSNGSVSLDEDEPLTRSSTKESRNG 508

[Close]

BLAST of Cp4.1LG18g00940.1 vs. TrEMBL
Match: B9RFT5_RICCO (Receptor serine-threonine protein kinase, putative OS=Ricinus communis GN=RCOM_1437210 PE=4 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 6.2e-19
Identity = 97/239 (40.59%), Postives = 131/239 (54.81%), Query Frame = 1


Query: 92  AQPFFRDPKRFPDLADPLLGRLFPEKDLNQAVAVTAMCLQEEAEVRPLIADVMTALSFLS 151
           AQP FRDPKRFPD+ADP+L + FPEKDLNQAVA+ AMCLQEEA  RPL++DV+TALSFLS
Sbjct: 283 AQPIFRDPKRFPDMADPVLNKRFPEKDLNQAVAIAAMCLQEEAPARPLMSDVVTALSFLS 342

Query: 152 TVPDENLTPLPYPPEPEPEDEEEDEEDAGDDYSSESSDSDTESRKIGKGDKDGASANFTS 211
              DE++ P P PP   P +E  + +    +   +SSDSD +  K  +  KD  S +  S
Sbjct: 343 MATDESI-PSPPPPSTPPSEEVPNHDQHKHEAGRDSSDSDPKDNK--ENHKDSDSDHKDS 402

Query: 212 ARNHDSDTSDDEDEQKNNSSKKKPKNKDSEQKRVVFKDDVQPNHSRKNSSSSSDGGSPRS 271
               + DTSDDED   ++    +  +K+S +             S   S SSS  GS R 
Sbjct: 403 ESEQEDDTSDDEDGDSDDYKDSRKWSKNSMR-------------SMDESVSSSHKGSKRI 462

Query: 272 SNASENRRI----DSRDSSFKMQKEASDIVNSTDSTLEHKSSRESKSGEGQNNSRSDSD 327
            + + +R +    +S+  S      +S      ++ L HKSS  SK G      RS S+
Sbjct: 463 QSRNASRSVKNSCESQYESVYSGHYSSRESRYHNARLSHKSSIGSKDGSVSGGERSSSN 505

[Close]

BLAST of Cp4.1LG18g00940.1 vs. TrEMBL
Match: R0G911_9BRAS (Uncharacterized protein OS=Capsella rubella GN=CARUB_v10015236mg PE=3 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 2.0e-17
Identity = 54/87 (62.07%), Postives = 64/87 (73.56%), Query Frame = 1


Query: 92  AQPFFRDPKRFPDLADPLLGRLFPEKDLNQAVAVTAMCLQEEAEVRPLIADVMTALSFLS 151
           AQP F++P RFP+LADP L  +FPEK LNQAVAV AMCLQEEA VRPL++DV+TAL FL 
Sbjct: 299 AQPVFKEPSRFPELADPSLEGVFPEKALNQAVAVAAMCLQEEATVRPLMSDVVTALGFLG 358

Query: 152 TVPDENLTPLPY---PPEPEPEDEEED 176
           T PD +L+   Y   PP+P  E   ED
Sbjct: 359 TAPDGSLSVPHYDDIPPQPSDETSAED 385

[Close]

BLAST of Cp4.1LG18g00940.1 vs. TrEMBL
Match: D7L628_ARALL (Kinase family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_340899 PE=3 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 2.0e-17
Identity = 52/86 (60.47%), Postives = 64/86 (74.42%), Query Frame = 1


Query: 92  AQPFFRDPKRFPDLADPLLGRLFPEKDLNQAVAVTAMCLQEEAEVRPLIADVMTALSFLS 151
           AQP F++P RFP+LADP L  +FPEK LNQAVAV AMCLQEEA VRPL++DV+TAL FL 
Sbjct: 298 AQPVFKEPSRFPELADPSLEGVFPEKALNQAVAVAAMCLQEEATVRPLMSDVVTALGFLG 357

Query: 152 TVPDENLTPLPYPPEPEPEDEEEDEE 178
           T PD +++   Y   P+P DE   E+
Sbjct: 358 TAPDGSISVPHYDDPPQPSDETSVED 383

[Close]

BLAST of Cp4.1LG18g00940.1 vs. TAIR10
Match: AT3G07070.1 (AT3G07070.1 Protein kinase superfamily protein)

HSP 1 Score: 98.2 bits (243), Expect = 1.0e-20
Identity = 52/86 (60.47%), Postives = 64/86 (74.42%), Query Frame = 1


Query: 92  AQPFFRDPKRFPDLADPLLGRLFPEKDLNQAVAVTAMCLQEEAEVRPLIADVMTALSFLS 151
           AQP F++P RFP+LADP L  +FPEK LNQAVAV AMCLQEEA VRPL++DV+TAL FL 
Sbjct: 298 AQPVFKEPSRFPELADPSLEGVFPEKALNQAVAVAAMCLQEEATVRPLMSDVVTALGFLG 357

Query: 152 TVPDENLTPLPYPPEPEPEDEEEDEE 178
           T PD +++   Y   P+P DE   E+
Sbjct: 358 TAPDGSISVPHYDDPPQPSDETSVED 383

[Close]

BLAST of Cp4.1LG18g00940.1 vs. TAIR10
Match: AT4G13190.1 (AT4G13190.1 Protein kinase superfamily protein)

HSP 1 Score: 94.7 bits (234), Expect = 1.1e-19
Identity = 46/61 (75.41%), Postives = 52/61 (85.25%), Query Frame = 1


Query: 92  AQPFFRDPKRFPDLADPLLGRLFPEKDLNQAVAVTAMCLQEEAEVRPLIADVMTALSFLS 151
           AQP FR+P RFP+LADPLL   FPEK LNQAVA+ AMCLQEE  VRPLI+DV+TALSF+S
Sbjct: 290 AQPIFREPNRFPELADPLLQGEFPEKSLNQAVAIAAMCLQEEPIVRPLISDVVTALSFMS 349

Query: 152 T 153
           T
Sbjct: 350 T 350

[Close]

BLAST of Cp4.1LG18g00940.1 vs. TAIR10
Match: AT5G16500.1 (AT5G16500.1 Protein kinase superfamily protein)

HSP 1 Score: 80.5 bits (197), Expect = 2.2e-15
Identity = 94/258 (36.43%), Postives = 133/258 (51.55%), Query Frame = 1


Query: 92  AQPFFRDPKRFPDLADPLLGRLFPEKDLNQAVAVTAMCLQEEAEVRPLIADVMTALSFLS 151
           AQP F+DPKR+PD+ADPLL + F E+ LNQAVA+T+MCLQEE   RPLI+DVM ALSFLS
Sbjct: 294 AQPIFKDPKRYPDMADPLLRKNFSERGLNQAVAITSMCLQEEPTARPLISDVMVALSFLS 353

Query: 152 TVPDENLTPLPYPPEPEPEDEEE----------------DEEDAGDDYSSESSDSDTESR 211
              ++ + P   P E   +                      +D G+  SS S   D E  
Sbjct: 354 MSTEDGI-PATVPMESFRDKSMSIALSRHGSCSVTPFCISRKDVGNKSSSSSDSEDEEEE 413

Query: 212 KIGKGDKDGASANFTSARNHDSDTSDDEDEQKNNSSKKKPKNKDSEQKRVVFKDDVQPNH 271
           K  K +K+  S +    R    +T+ D D++ +++S+K     D E      ++  Q   
Sbjct: 414 KEQKAEKEEESTS--KKRQEQEETATDSDDESDSNSEK-----DQE------EEQSQLEK 473

Query: 272 SRKNSSSSSDGGSPRSSNASENRRIDSRDSSFKMQKEASDIVNSTDSTLEHKSSRES-KS 331
           +R++SSSSSD G       SE R ID  +++ +  K +    +S +   E  SS+ S KS
Sbjct: 474 ARESSSSSSDSG-------SERRSIDETNATAQSLKISYSNYSSEEEDNEKLSSKSSCKS 528

Query: 332 GEGQNNSRSDSDGVSEHD 333
            E    SR DS    +HD
Sbjct: 534 NEESTFSRYDSG--RDHD 528

[Close]

BLAST of Cp4.1LG18g00940.1 vs. TAIR10
Match: AT3G24790.1 (AT3G24790.1 Protein kinase superfamily protein)

HSP 1 Score: 80.1 bits (196), Expect = 2.9e-15
Identity = 41/68 (60.29%), Postives = 49/68 (72.06%), Query Frame = 1


Query: 92  AQPFFRDPKRFPDLADPLLGRLFPEKDLNQAVAVTAMCLQEEAEVRPLIADVMTALSFLS 151
           A P FRDP R+  LADPLL   +PEK LNQA+AV AMCL EE  VRPL++DV+TALSFL 
Sbjct: 266 ALPIFRDPTRYWQLADPLLRGDYPEKSLNQAIAVAAMCLHEEPTVRPLMSDVITALSFLG 325

Query: 152 TVPDENLT 160
              + + T
Sbjct: 326 ASSNSSNT 333

[Close]

BLAST of Cp4.1LG18g00940.1 vs. TAIR10
Match: AT5G02800.1 (AT5G02800.1 Protein kinase superfamily protein)

HSP 1 Score: 73.6 bits (179), Expect = 2.7e-13
Identity = 44/108 (40.74%), Postives = 62/108 (57.41%), Query Frame = 1


Query: 60  KIDAQSF--TFRELATATKNFRQECLLGEGGF-GKAQPFFRDPKRFPDLADPLLGRLFPE 119
           K D  SF     E+ T  K        GE      A+P F+D ++F  +ADP+L   +P 
Sbjct: 257 KSDVYSFGVVLLEIITGRKAIDSSRSTGEQNLVAWARPLFKDRRKFSQMADPMLQGQYPP 316

Query: 120 KDLNQAVAVTAMCLQEEAEVRPLIADVMTALSFLSTVPDENLTPLPYP 165
           + L QA+AV AMC+QE+  +RPLIADV+TALS+L++   +   PL  P
Sbjct: 317 RGLYQALAVAAMCVQEQPNLRPLIADVVTALSYLAS---QKFDPLAQP 361

[Close]

BLAST of Cp4.1LG18g00940.1 vs. NCBI nr
Match: gi|778664208|ref|XP_004142598.2| (PREDICTED: serine/threonine-protein kinase At3g07070-like [Cucumis sativus])

HSP 1 Score: 298.1 bits (762), Expect = 1.9e-77
Identity = 221/341 (64.81%), Postives = 242/341 (70.97%), Query Frame = 1


Query: 24  ASSSSSGHRTPPPPKSPETTTPKPPEEVKDKPQEGEKIDAQSF--------TFRELATAT 83
           A     G ++P P +   T     PE  +   Q   K D  SF        T R+    T
Sbjct: 231 AKLGPGGDKSPLPSRVMGTYGYSAPEYTRGG-QLTSKSDIYSFGVVMLELITGRKAIDTT 290

Query: 84  KNFRQECLLGEGGFGKAQPFFRDPKRFPDLADPLLGRLFPEKDLNQAVAVTAMCLQEEAE 143
           K   ++ L+       AQPFFRDPKRFPDLADPLLGRLFPEKDLNQAVAV AMCLQEEAE
Sbjct: 291 KPNNEQNLVTW-----AQPFFRDPKRFPDLADPLLGRLFPEKDLNQAVAVAAMCLQEEAE 350

Query: 144 VRPLIADVMTALSFLSTVPDENLTPLPYPPEPEPEDEEEDEEDAGDDYSSESSDSDTESR 203
           VRPLI DVMTALSFLSTVPDENL PLPYPPEPEPE E E EE+  DD SSESSDSDTESR
Sbjct: 351 VRPLIGDVMTALSFLSTVPDENLRPLPYPPEPEPEPEPE-EENRDDDSSSESSDSDTESR 410

Query: 204 KIGKGDKDGASANFTSARNHDSDT-SDDEDE-QKNN--SSKKKPKNKDSEQKRVVFKDDV 263
           KIGK DKD ASANFTSARNH++DT SDDEDE +K N  SSKKKPKNK+SEQKRVVFKDD+
Sbjct: 411 KIGK-DKDCASANFTSARNHETDTSSDDEDEGEKENIKSSKKKPKNKESEQKRVVFKDDI 470

Query: 264 Q---PNHSRKNSSSSSDG-GSPRSSNASENRRIDS---------RDSSFKMQKEASDIVN 323
           Q   PNH+RKNSSSSSDG  SPRSSNASENRRIDS          DSSFK+Q E SD+VN
Sbjct: 471 QPPAPNHTRKNSSSSSDGSSSPRSSNASENRRIDSLESIRDSEGGDSSFKLQDEPSDVVN 530

Query: 324 STDSTLEHKSSRESKSG----EGQNNSRSDSDGVSEHDGKD 336
           S DSTLEH SSRES +G    E  N   SDS+  SEH G++
Sbjct: 531 SNDSTLEHISSRESHTGDEEYEAHNEYSSDSEEGSEHGGEE 563

[Close]

BLAST of Cp4.1LG18g00940.1 vs. NCBI nr
Match: gi|659086070|ref|XP_008443749.1| (PREDICTED: serine/threonine-protein kinase At3g07070-like isoform X2 [Cucumis melo])

HSP 1 Score: 197.2 bits (500), Expect = 4.5e-47
Identity = 139/227 (61.23%), Postives = 157/227 (69.16%), Query Frame = 1


Query: 24  ASSSSSGHRTPPPPKSPETTTPKPPEEVKDKPQEGEKIDAQSF--------TFRELATAT 83
           A     G ++P P +   T     PE  +   Q   K D  SF        T R+    T
Sbjct: 257 AKLGPGGDKSPLPSRVMGTYGYSAPEYTRGG-QLTSKSDIYSFGVVMLELITGRKAIDTT 316

Query: 84  KNFRQECLLGEGGFGKAQPFFRDPKRFPDLADPLLGRLFPEKDLNQAVAVTAMCLQEEAE 143
           K   ++ L+       AQPFFRDPKRFPDLADPLLGRLFPEKDLNQAVAV AMCLQEEAE
Sbjct: 317 KPNNEQNLVTW-----AQPFFRDPKRFPDLADPLLGRLFPEKDLNQAVAVAAMCLQEEAE 376

Query: 144 VRPLIADVMTALSFLSTVPDENLTPLPYPPEPEPEDEEEDEEDAGDDYSSESSDSDTESR 203
           VRPLI DVMTALSFLSTVPDENL PLPYPPEPEPE EEE+ +   DD SSESSDSDTESR
Sbjct: 377 VRPLIGDVMTALSFLSTVPDENLRPLPYPPEPEPEPEEENRD---DDSSSESSDSDTESR 436

Query: 204 KIGKGDKDGASANFTSARNHDSDT-SDDEDEQKNNSSKKKPKNKDSE 242
           KIGK DK+GASANFTSARNH++DT SDDEDE +  + K   KN+ ++
Sbjct: 437 KIGK-DKEGASANFTSARNHETDTSSDDEDEGEKENIKSSKKNRKTK 473

[Close]

BLAST of Cp4.1LG18g00940.1 vs. NCBI nr
Match: gi|659086068|ref|XP_008443748.1| (PREDICTED: serine/threonine-protein kinase At3g07070-like isoform X1 [Cucumis melo])

HSP 1 Score: 197.2 bits (500), Expect = 4.5e-47
Identity = 139/227 (61.23%), Postives = 157/227 (69.16%), Query Frame = 1


Query: 24  ASSSSSGHRTPPPPKSPETTTPKPPEEVKDKPQEGEKIDAQSF--------TFRELATAT 83
           A     G ++P P +   T     PE  +   Q   K D  SF        T R+    T
Sbjct: 236 AKLGPGGDKSPLPSRVMGTYGYSAPEYTRGG-QLTSKSDIYSFGVVMLELITGRKAIDTT 295

Query: 84  KNFRQECLLGEGGFGKAQPFFRDPKRFPDLADPLLGRLFPEKDLNQAVAVTAMCLQEEAE 143
           K   ++ L+       AQPFFRDPKRFPDLADPLLGRLFPEKDLNQAVAV AMCLQEEAE
Sbjct: 296 KPNNEQNLVTW-----AQPFFRDPKRFPDLADPLLGRLFPEKDLNQAVAVAAMCLQEEAE 355

Query: 144 VRPLIADVMTALSFLSTVPDENLTPLPYPPEPEPEDEEEDEEDAGDDYSSESSDSDTESR 203
           VRPLI DVMTALSFLSTVPDENL PLPYPPEPEPE EEE+ +   DD SSESSDSDTESR
Sbjct: 356 VRPLIGDVMTALSFLSTVPDENLRPLPYPPEPEPEPEEENRD---DDSSSESSDSDTESR 415

Query: 204 KIGKGDKDGASANFTSARNHDSDT-SDDEDEQKNNSSKKKPKNKDSE 242
           KIGK DK+GASANFTSARNH++DT SDDEDE +  + K   KN+ ++
Sbjct: 416 KIGK-DKEGASANFTSARNHETDTSSDDEDEGEKENIKSSKKNRKTK 452

[Close]

BLAST of Cp4.1LG18g00940.1 vs. NCBI nr
Match: gi|703148310|ref|XP_010109313.1| (Serine/threonine-protein kinase [Morus notabilis])

HSP 1 Score: 105.5 bits (262), Expect = 1.8e-19
Identity = 89/234 (38.03%), Postives = 130/234 (55.56%), Query Frame = 1


Query: 92  AQPFFRDPKRFPDLADPLLGRLFPEKDLNQAVAVTAMCLQEEAEVRPLIADVMTALSFLS 151
           AQPFFRDPK+FPDLADPLL R FPEKDLNQ VA+ AMCLQ+EA  RPLI+D++T LSFLS
Sbjct: 283 AQPFFRDPKKFPDLADPLLNRQFPEKDLNQVVAIAAMCLQDEASARPLISDIVTTLSFLS 342

Query: 152 TVPDENLTPLPYPPEPEPEDE--------EEDEEDAGDDYSSESSDS-DTESRKIGKGDK 211
           T P E +   P P + +  D         ++ E D  DD   ESS+S ++ES        
Sbjct: 343 TTPSECIQTAPTPQDGKDSDSKSSKHENAKDQESDCSDDEDGESSESQESESSDYEDVKS 402

Query: 212 DGASANFTSARNHDSDTSDDEDEQKNNSSKKKPKNKDSEQKRVVFKDDVQPNHSRKNSSS 271
              + ++ S+R      S+D+D +  +SS+K     D           V  + S  +++S
Sbjct: 403 RDEAKDWQSSRKSSMAGSEDDDNESISSSQKSSSGSDD--------GSVSSSQSGSSTNS 462

Query: 272 SSDGGSPRSSNASENRRIDSRDSSFKMQKEASDIVN-STDSTLEHKSSRESKSG 316
            +   S R+S+   NR + + +S    +K ++  V+   D  L   S++ES++G
Sbjct: 463 KASSVSSRNSSIGSNRPVSNFNSQRSSRKSSNGSVSLDEDEPLTRSSTKESRNG 508

[Close]

BLAST of Cp4.1LG18g00940.1 vs. NCBI nr
Match: gi|223548565|gb|EEF50056.1| (receptor serine-threonine protein kinase, putative [Ricinus communis])

HSP 1 Score: 103.2 bits (256), Expect = 8.9e-19
Identity = 97/239 (40.59%), Postives = 131/239 (54.81%), Query Frame = 1


Query: 92  AQPFFRDPKRFPDLADPLLGRLFPEKDLNQAVAVTAMCLQEEAEVRPLIADVMTALSFLS 151
           AQP FRDPKRFPD+ADP+L + FPEKDLNQAVA+ AMCLQEEA  RPL++DV+TALSFLS
Sbjct: 283 AQPIFRDPKRFPDMADPVLNKRFPEKDLNQAVAIAAMCLQEEAPARPLMSDVVTALSFLS 342

Query: 152 TVPDENLTPLPYPPEPEPEDEEEDEEDAGDDYSSESSDSDTESRKIGKGDKDGASANFTS 211
              DE++ P P PP   P +E  + +    +   +SSDSD +  K  +  KD  S +  S
Sbjct: 343 MATDESI-PSPPPPSTPPSEEVPNHDQHKHEAGRDSSDSDPKDNK--ENHKDSDSDHKDS 402

Query: 212 ARNHDSDTSDDEDEQKNNSSKKKPKNKDSEQKRVVFKDDVQPNHSRKNSSSSSDGGSPRS 271
               + DTSDDED   ++    +  +K+S +             S   S SSS  GS R 
Sbjct: 403 ESEQEDDTSDDEDGDSDDYKDSRKWSKNSMR-------------SMDESVSSSHKGSKRI 462

Query: 272 SNASENRRI----DSRDSSFKMQKEASDIVNSTDSTLEHKSSRESKSGEGQNNSRSDSD 327
            + + +R +    +S+  S      +S      ++ L HKSS  SK G      RS S+
Sbjct: 463 QSRNASRSVKNSCESQYESVYSGHYSSRESRYHNARLSHKSSIGSKDGSVSGGERSSSN 505

The following BLAST results are available for this feature:

BLAST of Cp4.1LG18g00940.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
Y3707_ARATH	1.8e-19	60.47	Serine/threonine-protein kinase At3g07070 OS=Arabidopsis thaliana GN=At3g07070 P...	[more]
LIPG1_ARATH	3.9e-14	36.43	Receptor-like kinase LIP1 OS=Arabidopsis thaliana GN=LIP1 PE=1 SV=1	[more]
CDL1_ARATH	4.7e-12	40.74	Serine/threonine-protein kinase CDL1 OS=Arabidopsis thaliana GN=CDL1 PE=1 SV=1	[more]
LIPG2_ARATH	3.1e-11	48.91	Receptor-like kinase LIP2 OS=Arabidopsis thaliana GN=LIP2 PE=1 SV=1	[more]
RLCK7_ARATH	2.4e-08	40.45	Probable serine/threonine-protein kinase RLCKVII OS=Arabidopsis thaliana GN=At1g...	[more]

BLAST of Cp4.1LG18g00940.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LY64_CUCSA	1.3e-77	64.81	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G662820 PE=4 SV=1	[more]
W9SA67_9ROSA	1.3e-19	38.03	Serine/threonine-protein kinase OS=Morus notabilis GN=L484_006459 PE=3 SV=1	[more]
B9RFT5_RICCO	6.2e-19	40.59	Receptor serine-threonine protein kinase, putative OS=Ricinus communis GN=RCOM_1...	[more]
R0G911_9BRAS	2.0e-17	62.07	Uncharacterized protein OS=Capsella rubella GN=CARUB_v10015236mg PE=3 SV=1	[more]
D7L628_ARALL	2.0e-17	60.47	Kinase family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_340899 P...	[more]

BLAST of Cp4.1LG18g00940.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G07070.1	1.0e-20	60.47	Protein kinase superfamily protein	[more]
AT4G13190.1	1.1e-19	75.41	Protein kinase superfamily protein	[more]
AT5G16500.1	2.2e-15	36.43	Protein kinase superfamily protein	[more]
AT3G24790.1	2.9e-15	60.29	Protein kinase superfamily protein	[more]
AT5G02800.1	2.7e-13	40.74	Protein kinase superfamily protein	[more]

BLAST of Cp4.1LG18g00940.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778664208\|ref\|XP_004142598.2\|	1.9e-77	64.81	PREDICTED: serine/threonine-protein kinase At3g07070-like [Cucumis sativus]	[more]
gi\|659086070\|ref\|XP_008443749.1\|	4.5e-47	61.23	PREDICTED: serine/threonine-protein kinase At3g07070-like isoform X2 [Cucumis me...	[more]
gi\|659086068\|ref\|XP_008443748.1\|	4.5e-47	61.23	PREDICTED: serine/threonine-protein kinase At3g07070-like isoform X1 [Cucumis me...	[more]
gi\|703148310\|ref\|XP_010109313.1\|	1.8e-19	38.03	Serine/threonine-protein kinase [Morus notabilis]	[more]
gi\|223548565\|gb\|EEF50056.1\|	8.9e-19	40.59	receptor serine-threonine protein kinase, putative [Ricinus communis]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR013320	ConA-like_dom_sf

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
biological_process	GO:0009069	serine family amino acid metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004674	protein serine/threonine kinase activity
molecular_function	GO:0050254	rhodopsin kinase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cp4.1LG18g00940	Cp4.1LG18g00940	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG18g00940.1:cds:001	Cp4.1LG18g00940.1:cds:001	CDS
Cp4.1LG18g00940.1:cds:002	Cp4.1LG18g00940.1:cds:002	CDS
Cp4.1LG18g00940.1:cds:003	Cp4.1LG18g00940.1:cds:003	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG18g00940.1	Cp4.1LG18g00940.1-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013320	Concanavalin A-like lectin/glucanase domain	GENE3D	G3DSA:2.60.120.200		coord: 61..89 score: 1.
None	No IPR available	PANTHER	PTHR27001	FAMILY NOT NAMED	coord: 92..186 score: 2.3
None	No IPR available	PANTHER	PTHR27001:SF48	PROTEIN KINASE FAMILY PROTEIN	coord: 92..186 score: 2.3