Cp4.1LG17g09460.1 (mRNA) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG17g09460.1
Type	mRNA
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	Cytochrome P450
Location	Cp4.1LG17 : 7304177 .. 7305127 (+)
Sequence length	951

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGACACCACATTCCTGTATTCAGTTCTGGGCATTGCTCTCCTGGTTGCCATAAGCTATGCTTTGAAACCAAAGTCCCGCAAAAATCTCCCTCCAACGCCACCCTCTATCCCAATTATAGGCCATCTCCATCTCCTCAAGCCCCCAGTTCACAGAAGCTTATTAACTCTCTCACAGAAGTATGGCCCAGTGCTTTCCCTTCGTTTCGGCTCTCGTTTAGTCGTTGTTGTCTCGTCCATTGACGCAGCCGAAGAATGCTTCACGAAAAACGATGTCGTTTTTGCTAACCGGCCAGACTTTGCTGTGAGTAAGTGCTTGAGTTACAACCACACCACCCTTGGAGCCGCTCCCTATGGTGACCACTGGCGAAAACTTCGCCGAGTGAGCGCTCTCGAAATACTCTCTGCCAATCGTCTCAACACGAATTCCGCCATTCGAAGAGATGAAGTGAGGAGATTGGTGAATAAACTATCTCAATCTTCGAGAGATGATTTTGCGAGAGTGGAGTTTAAATCCATGGTGAAAGAACTGACGCTGAATATAACGATGAGAATGGTGGCCGGGAAGCGATATTATGGGGAAGAAACAGATGAAAGCAGTGAAGCCATGACGTTTCGAGCTATTGTCGATGAGCTGTTCGAGTTCATGTTAGCGCAATATCCAGCAGACTTTTTGCCGATTTTGAAGTATATTGATTTCCAGGGCTTCATGAAGAGAGCCAAGAAGCTGATCAAAAGGGTGGATGCATTTTGGCAAGGAATCGTTGATGAACATCGAACCAATCAAATCCATAATCAAGAAATGGAGAATAGTATGGTTGCCCATTTCCTCAAGTTGCAGGAGTCGCAGCCTCAATATTACACTGATGACATTATCAAAGGCCTCATTTTGGTCCGTGCTCCCAAATATTTACTTACCGTTACAAGTTTAATCCACGAACTTTTATAA

mRNA sequence

ATGGAAGACACCACATTCCTGTATTCAGTTCTGGGCATTGCTCTCCTGGTTGCCATAAGCTATGCTTTGAAACCAAAGTCCCGCAAAAATCTCCCTCCAACGCCACCCTCTATCCCAATTATAGGCCATCTCCATCTCCTCAAGCCCCCAGTTCACAGAAGCTTATTAACTCTCTCACAGAAGTATGGCCCAGTGCTTTCCCTTCGTTTCGGCTCTCGTTTAGTCGTTGTTGTCTCGTCCATTGACGCAGCCGAAGAATGCTTCACGAAAAACGATGTCGTTTTTGCTAACCGGCCAGACTTTGCTGTGAGTAAGTGCTTGAGTTACAACCACACCACCCTTGGAGCCGCTCCCTATGGTGACCACTGGCGAAAACTTCGCCGAGTGAGCGCTCTCGAAATACTCTCTGCCAATCGTCTCAACACGAATTCCGCCATTCGAAGAGATGAAGTGAGGAGATTGGTGAATAAACTATCTCAATCTTCGAGAGATGATTTTGCGAGAGTGGAGTTTAAATCCATGGTGAAAGAACTGACGCTGAATATAACGATGAGAATGGTGGCCGGGAAGCGATATTATGGGGAAGAAACAGATGAAAGCAGTGAAGCCATGACGTTTCGAGCTATTGTCGATGAGCTGTTCGAGTTCATGTTAGCGCAATATCCAGCAGACTTTTTGCCGATTTTGAAGTATATTGATTTCCAGGGCTTCATGAAGAGAGCCAAGAAGCTGATCAAAAGGGTGGATGCATTTTGGCAAGGAATCGTTGATGAACATCGAACCAATCAAATCCATAATCAAGAAATGGAGAATAGTATGGTTGCCCATTTCCTCAAGTTGCAGGAGTCGCAGCCTCAATATTACACTGATGACATTATCAAAGGCCTCATTTTGGTCCGTGCTCCCAAATATTTACTTACCGTTACAAGTTTAATCCACGAACTTTTATAA

Coding sequence (CDS)

ATGGAAGACACCACATTCCTGTATTCAGTTCTGGGCATTGCTCTCCTGGTTGCCATAAGCTATGCTTTGAAACCAAAGTCCCGCAAAAATCTCCCTCCAACGCCACCCTCTATCCCAATTATAGGCCATCTCCATCTCCTCAAGCCCCCAGTTCACAGAAGCTTATTAACTCTCTCACAGAAGTATGGCCCAGTGCTTTCCCTTCGTTTCGGCTCTCGTTTAGTCGTTGTTGTCTCGTCCATTGACGCAGCCGAAGAATGCTTCACGAAAAACGATGTCGTTTTTGCTAACCGGCCAGACTTTGCTGTGAGTAAGTGCTTGAGTTACAACCACACCACCCTTGGAGCCGCTCCCTATGGTGACCACTGGCGAAAACTTCGCCGAGTGAGCGCTCTCGAAATACTCTCTGCCAATCGTCTCAACACGAATTCCGCCATTCGAAGAGATGAAGTGAGGAGATTGGTGAATAAACTATCTCAATCTTCGAGAGATGATTTTGCGAGAGTGGAGTTTAAATCCATGGTGAAAGAACTGACGCTGAATATAACGATGAGAATGGTGGCCGGGAAGCGATATTATGGGGAAGAAACAGATGAAAGCAGTGAAGCCATGACGTTTCGAGCTATTGTCGATGAGCTGTTCGAGTTCATGTTAGCGCAATATCCAGCAGACTTTTTGCCGATTTTGAAGTATATTGATTTCCAGGGCTTCATGAAGAGAGCCAAGAAGCTGATCAAAAGGGTGGATGCATTTTGGCAAGGAATCGTTGATGAACATCGAACCAATCAAATCCATAATCAAGAAATGGAGAATAGTATGGTTGCCCATTTCCTCAAGTTGCAGGAGTCGCAGCCTCAATATTACACTGATGACATTATCAAAGGCCTCATTTTGGTCCGTGCTCCCAAATATTTACTTACCGTTACAAGTTTAATCCACGAACTTTTATAA

Protein sequence

MEDTTFLYSVLGIALLVAISYALKPKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMVKELTLNITMRMVAGKRYYGEETDESSEAMTFRAIVDELFEFMLAQYPADFLPILKYIDFQGFMKRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILVRAPKYLLTVTSLIHELL

Homology

[Close]

BLAST of Cp4.1LG17g09460.1 vs. Swiss-Prot
Match: C81E1_GLYEC (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 296.6 bits (758), Expect = 3.3e-79
Identity = 150/293 (51.19%), Postives = 204/293 (69.62%), Query Frame = 1


Query: 8   YSVLGIALLVAISYALKPKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQKYGPVLS 67
           YSV  +AL    +  ++ +  KNLPP PPS+PIIG+LH LK P+HR+   LS+KYG V S
Sbjct: 9   YSVFYLALFFIFNIVIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFS 68

Query: 68  LRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYGDHWRKLR 127
           L FGSRLVVVVSS    ++CFTKNDVV ANRP F   K + YN+TTLG+  YG+HWR LR
Sbjct: 69  LWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLR 128

Query: 128 RVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMVKELTLNITMRMV 187
           R++AL++LS +R+N+ S IRRDE +RL+ +L+  S  +FA +E  S + ++T N  MRM+
Sbjct: 129 RITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRMI 188

Query: 188 AGKRYYGEETDES--SEAMTFRAIVDELFEFMLAQYPADFLPILKYIDFQGFMKRAKKLI 247
           +GKRYYGE+ D S   EA  FR +V EL +   A    DF+P+L+++DF+   KR K + 
Sbjct: 189 SGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDIS 248

Query: 248 KRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLIL 299
            + DAF +G+++EHRT     +E  N+M+ H L LQ+SQP+YYTD IIKGL L
Sbjct: 249 GKTDAFLRGLIEEHRT----KKERANTMIDHLLNLQDSQPEYYTDQIIKGLAL 297

[Close]

BLAST of Cp4.1LG17g09460.1 vs. Swiss-Prot
Match: C81E9_MEDTR (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 2.8e-78
Identity = 157/322 (48.76%), Postives = 212/322 (65.84%), Query Frame = 1


Query: 6   FLYSVLGIALLVAISYALKPKSR--KNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQKYG 65
           F YS+L ++ ++ I   LK  SR  KNLPP PP+IPIIG+LH LK P+HR+  TLSQ YG
Sbjct: 3   FYYSLLSLSFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYG 62

Query: 66  PVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYGDHW 125
            + SL FGSRLVVVVSS   A ECFTKND++ ANRP F   K + YN+TTLG+A YGDHW
Sbjct: 63  DIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHW 122

Query: 126 RKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQ---SSRDDFARVEFKSMVKELTL 185
           R LRR++ +++LS NRLN+   +RRDE  RL+ KL +   S    F +VE +  + E+T 
Sbjct: 123 RNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTF 182

Query: 186 NITMRMVAGKRYYGEETDES--SEAMTFRAIVDELFEFMLAQYPADFLPILKYIDFQGFM 245
           N  MRM++GKRYYG++ D S   EA  FR I+ E+   + A    DFLP+L+ +D     
Sbjct: 183 NAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLE 242

Query: 246 KRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLI- 305
           KR K++ KR +AF +G+++EHR   IH+     +M+ H LKL ESQP+YY+D +IKGLI 
Sbjct: 243 KRCKRIAKRSNAFLEGLIEEHRRGNIHSD--GGTMIDHLLKLSESQPEYYSDHLIKGLIQ 302

Query: 306 ---LVRAPKYLLTVTSLIHELL 317
              L       +T+  ++ ELL
Sbjct: 303 GMLLAGTDTSAVTIEWVMSELL 322

[Close]

BLAST of Cp4.1LG17g09460.1 vs. Swiss-Prot
Match: C81E8_MEDTR (Cytochrome P450 81E8 OS=Medicago truncatula GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 1.4e-77
Identity = 154/300 (51.33%), Postives = 199/300 (66.33%), Query Frame = 1


Query: 4   TTFLYSVLGIALLVAISYALKPKSRK--NLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQK 63
           T +L  ++ +  L+         SRK  NLPP P  +PIIG+LH LK P+H +  TLSQK
Sbjct: 3   TFYLSLIISLFFLIITLKVFFNTSRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQK 62

Query: 64  YGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYGD 123
           YG + SL FGSRLVVVVSS+  A+ECFTKND+V ANRP F   K + YN+TT+  +PYGD
Sbjct: 63  YGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGD 122

Query: 124 HWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMVKELTLN 183
           HWR LRR+ ++EILS++RLN+   IRRDE+ RL+ KL+Q S + F  VE + M  E+T N
Sbjct: 123 HWRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFN 182

Query: 184 ITMRMVAGKRYYGEETDES--SEAMTFRAIVDELFEFMLAQYPADFLPILKYIDFQGFMK 243
             MRMV+GKRYYG + D S   EA  FR I+ E+     A    DFL  L++ DF G  K
Sbjct: 183 TIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEK 242

Query: 244 RAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILV 300
           R KK+ KR DAF QG++DEHR      +   N+M+ H L  Q+SQP+YYTD IIKGL++V
Sbjct: 243 RLKKISKRTDAFLQGLIDEHR----FGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVV 298

[Close]

BLAST of Cp4.1LG17g09460.1 vs. Swiss-Prot
Match: C81E7_MEDTR (Isoflavone 2'-hydroxylase OS=Medicago truncatula GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.2e-73
Identity = 142/293 (48.46%), Postives = 199/293 (67.92%), Query Frame = 1


Query: 8   YSVLGIALLVAISYALKPKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQKYGPVLS 67
           YS+  +++   I    + +  KNLPP P S+PIIG+LH LK P++R+   L++KYG V+S
Sbjct: 9   YSLFYLSIFFIIRLLFQSRKFKNLPPGPTSLPIIGNLHHLKRPLNRTFKALTEKYGNVIS 68

Query: 68  LRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYGDHWRKLR 127
           L FGSRLVVVVSS+   +ECFTKNDVV ANRP F   K + YN+TTLG+  YG+HWR LR
Sbjct: 69  LWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLR 128

Query: 128 RVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMVKELTLNITMRMV 187
           R+++L++LS +R+N  + IRRDE +RL+ KL++ S   FA VE      ++T N  MRM+
Sbjct: 129 RITSLDVLSNHRINNFAPIRRDETQRLIKKLAEDSSTKFAEVELTFRFFDMTFNNIMRMI 188

Query: 188 AGKRYYGEETD--ESSEAMTFRAIVDELFEFMLAQYPADFLPILKYIDFQGFMKRAKKLI 247
           +GKRYYG++ D  E  EA  FR +V EL +   A    DF+P+LK++DF+   KR K++ 
Sbjct: 189 SGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLEKRVKRIG 248

Query: 248 KRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLIL 299
           ++ D F  G++ E R+ +    E  N+M+ H L +QESQP+YYTD IIKGL L
Sbjct: 249 EKNDVFLSGLLQEQRSKK----ERTNTMIDHLLNMQESQPEYYTDTIIKGLCL 297

[Close]

BLAST of Cp4.1LG17g09460.1 vs. Swiss-Prot
Match: C8D11_ARATH (Cytochrome P450 81D11 OS=Arabidopsis thaliana GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 3.2e-66
Identity = 144/303 (47.52%), Postives = 194/303 (64.03%), Query Frame = 1


Query: 1   MEDTTFLYSVLGIALLVAISYALKPKSRK-NLPPTPPS-IPIIGHLHLLKPPVHRSLLTL 60
           ME    + S+    + +++     P+ RK NLPP+P    PIIGHLHLLK P+HR  L+L
Sbjct: 8   METIYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSL 67

Query: 61  SQKYG--PVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGA 120
           S+      + SL  GSRLV VVSS   AEECFTKNDVV ANRP+F V K + YN TT+  
Sbjct: 68  SESLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVG 127

Query: 121 APYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMVK 180
           A YGD WR LRR+  +EI S+ RLN+  +IR+DE+RRL+  L+++S+  F +VE K +  
Sbjct: 128 AAYGDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFM 187

Query: 181 ELTLNITMRMVAGKRYYGEETDESSEAMTFRAIVDELFEFMLAQYPADFLPILKYIDFQG 240
            LT+N  +RMVAGKR+YG+ T+  +EA   R ++ E+     A   AD+ PIL+Y+    
Sbjct: 188 CLTINNIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYVT--N 247

Query: 241 FMKRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGL 300
           + K  KKL  RVD F Q +V+E R  ++      N+M+ H L LQE+QP YYTD IIKG+
Sbjct: 248 YEKHVKKLAGRVDEFLQSLVNEKRVEKVKG----NTMIDHLLSLQETQPDYYTDVIIKGI 304

[Close]

BLAST of Cp4.1LG17g09460.1 vs. TrEMBL
Match: A0A097IYP1_CUCSA (Cytochrome P450 OS=Cucumis sativus GN=Csa6G088160 PE=2 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 6.3e-122
Identity = 223/303 (73.60%), Postives = 254/303 (83.83%), Query Frame = 1


Query: 1   MEDTTFLYSV-----LGIALLVAISYALKPKSRKNLPPTPPSIPIIGHLHLLKPPVHRSL 60
           MED+   YS      + + +L  I Y LK  S KNLPP+PPSIPIIGHLHLLK PVHR+L
Sbjct: 1   MEDSILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 60

Query: 61  LTLSQKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLG 120
            TLSQKYGPVLSLRFGSR VVVV+S++AAEECF KND++FANRP+FAVS CLSYN TTLG
Sbjct: 61  QTLSQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLG 120

Query: 121 AAPYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMV 180
           AAPYGDHWRKLRRVS LEIL ++RLN N  IRRDEVRR + K+ + SRD F +VEFK +V
Sbjct: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLV 180

Query: 181 KELTLNITMRMVAGKRYYGEETDESSEAMTFRAIVDELFEFMLAQYPADFLPILKYIDFQ 240
           KELT+N+TMRMVAGKRY+GEE  +SSEA TF+AIV ELFEF L+ YPADFLPILKYID Q
Sbjct: 181 KELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 240

Query: 241 GFMKRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKG 299
           GFMKRAKKLI RVDAFWQG++DEHR  +I N+EM+N MVAHFLKLQE+QP YYTDDIIKG
Sbjct: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 300

[Close]

BLAST of Cp4.1LG17g09460.1 vs. TrEMBL
Match: A0A061DI90_THECC (Cytochrome P450, family 81, subfamily D, polypeptide 5, putative OS=Theobroma cacao GN=TCM_000715 PE=3 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 3.7e-90
Identity = 164/299 (54.85%), Postives = 223/299 (74.58%), Query Frame = 1


Query: 1   MEDTTFLYSVLGIALLVAISYALKPKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQ 60
           ME T        + LLVAI + L+ +  KNLPP+P ++PI+GHLHLLK P+HR+L TLSQ
Sbjct: 30  MEQTLLCSLASVLFLLVAIRFLLQARKHKNLPPSPFALPILGHLHLLKEPLHRTLFTLSQ 89

Query: 61  KYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYG 120
           K+GP+ SLRFGSRL VV++S  A EECF KND+  ANRP   + K + YN+T L  A YG
Sbjct: 90  KHGPIFSLRFGSRLAVVITSPSAVEECFNKNDITLANRPRLIMGKYVGYNYTILALASYG 149

Query: 121 DHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMVKELTL 180
           DHWR LRR++ LEILS+N L+ +S+IRRDE+  L+ +L + S + FA+VE K++  ELT 
Sbjct: 150 DHWRNLRRLATLEILSSNNLSMSSSIRRDEINSLIRRLYRVSVNGFAKVELKTLFAELTF 209

Query: 181 NITMRMVAGKRYYGEETDESSEAMTFRAIVDELFEFMLAQYPADFLPILKYIDFQGFMKR 240
           NI MR+VAGKRYYG+E   S EA  FRA+++ELFE  ++ YP DFLP+L ++D+ G++K+
Sbjct: 210 NIIMRIVAGKRYYGDEVRGSEEARGFRAMIEELFELTVSSYPGDFLPLLSWVDYNGYIKK 269

Query: 241 AKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILV 300
            K + K  DAF QG++DEHR N+  N E++N+M++H L LQ+SQP+YYTD+IIKGL+LV
Sbjct: 270 VKLVAKNADAFTQGLIDEHRRNK-GNLELKNTMISHLLTLQKSQPEYYTDEIIKGLVLV 327

[Close]

BLAST of Cp4.1LG17g09460.1 vs. TrEMBL
Match: A0A061EFX9_THECC (Cytochrome P450, family 81, subfamily D, polypeptide 8, putative OS=Theobroma cacao GN=TCM_019144 PE=3 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 9.2e-89
Identity = 173/301 (57.48%), Postives = 224/301 (74.42%), Query Frame = 1


Query: 1   MEDTTFLYSVLGIALL-VAISYALKPK-SRKNLPPTPPSIPIIGHLHLLKPPVHRSLLTL 60
           ME+TT LYS L + LL +++++  + K S KNLPP+PPS+PI+GHLHLLKP +HRS  TL
Sbjct: 1   MEETTILYSSLSLVLLLISLNFFFQSKKSHKNLPPSPPSLPILGHLHLLKPLIHRSYHTL 60

Query: 61  SQKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAP 120
           SQKYGP+ S++ GSRL VVVSS  AAEECFTKND++ ANRP     K L YN+TTL A+P
Sbjct: 61  SQKYGPIFSIQLGSRLQVVVSSSAAAEECFTKNDIILANRPKLIQGKHLGYNYTTLIASP 120

Query: 121 YGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMVKEL 180
           YGDHWR LRR+ A+EI S++RLNT+ ++RRDE+RRL+ KLS+ SR  F +VE KSM+ EL
Sbjct: 121 YGDHWRNLRRIGAIEIFSSSRLNTSISVRRDEIRRLLLKLSRDSRQHFTKVELKSMLSEL 180

Query: 181 TLNITMRMVAGKRYYGEETDESSEAMTFRAIVDELFEFMLAQYPADFLPILKYIDFQGFM 240
           T N  MRMVAGKRYYG+E    +EA  FR ++ E+ +   A  PADF P+L +   Q + 
Sbjct: 181 TFNNIMRMVAGKRYYGDEVTNEAEAKEFRDLIAEVVKHGGAANPADFFPMLSWFG-QWYE 240

Query: 241 KRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLIL 300
           K+ KKL KR+D F+Q ++DEHR+ +  N     SM+AH L LQES+P YYTDDI+KGL+L
Sbjct: 241 KKDKKLGKRMDVFFQKLIDEHRSYRQEN----TSMIAHLLSLQESEPHYYTDDILKGLVL 296

[Close]

BLAST of Cp4.1LG17g09460.1 vs. TrEMBL
Match: A0A067KJK1_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_12475 PE=3 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 6.0e-88
Identity = 171/300 (57.00%), Postives = 219/300 (73.00%), Query Frame = 1


Query: 1   MEDTTFLY-SVLGIALLVAISYALKPKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLLTLS 60
           ME  T LY S   + L + I +      RKNLPP+PPS PIIGHL+LLKPP+HR L +L+
Sbjct: 1   MEHYTVLYFSSSFLLLFLVIKFCQSKVHRKNLPPSPPSFPIIGHLYLLKPPMHRILHSLA 60

Query: 61  QKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPY 120
           Q YGPV+ LRFGSR +++VSS+  AEECFTKND+VFANRP   ++K +SYN+TT+  APY
Sbjct: 61  QTYGPVIFLRFGSRPLIIVSSLSIAEECFTKNDIVFANRPRLLMAKHISYNYTTMTQAPY 120

Query: 121 GDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMVKELT 180
           GDHWR LRRV A+EI SA+RLNT  +IR+DE++RL+ KLS +S  DF +VE KSM +ELT
Sbjct: 121 GDHWRNLRRVGAIEIFSAHRLNTLLSIRKDEIKRLIMKLSINSMQDFCKVELKSMFQELT 180

Query: 181 LNITMRMVAGKRYYGEETDESSEAMTFRAIVDELFEFMLAQYPADFLPILKYIDFQGFMK 240
            NI +RMVAGKRYYG++     EA  FR ++ ++  +  A  PADFLPIL +ID     K
Sbjct: 181 FNIMIRMVAGKRYYGDDVSNEEEAREFRKLMKKVVAYAGASNPADFLPILNWIDGGYLRK 240

Query: 241 RAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILV 300
           R K   KR D F+QG++DEHR+ +  N E +N+M+ H L LQESQP+YYTD+IIKGLI V
Sbjct: 241 RIKGFAKRFDNFFQGLIDEHRSKK-GNLENKNTMIDHLLSLQESQPEYYTDEIIKGLIQV 299

[Close]

BLAST of Cp4.1LG17g09460.1 vs. TrEMBL
Match: A0A061EGZ3_THECC (Cytochrome P450, family 81, subfamily D, polypeptide 8 OS=Theobroma cacao GN=TCM_019148 PE=3 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 2.3e-87
Identity = 171/301 (56.81%), Postives = 223/301 (74.09%), Query Frame = 1


Query: 1   MEDTTFLYSVLGIA-LLVAISYALKPK-SRKNLPPTPPSIPIIGHLHLLKPPVHRSLLTL 60
           ME+TT LYS L +  LL+++++  + K S KNLPP+PPS PI+GHLHLLKPP+HRS   L
Sbjct: 1   MEETTILYSSLSLVFLLISLNFFFQSKKSHKNLPPSPPSRPILGHLHLLKPPIHRSYHAL 60

Query: 61  SQKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAP 120
           SQKYGP+ SL+ GSRL VVVSS  AAEECFTKND++ ANRP     K L YN+TT+ A+P
Sbjct: 61  SQKYGPIFSLQLGSRLQVVVSSSAAAEECFTKNDIILANRPRLIRGKHLGYNYTTVSASP 120

Query: 121 YGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMVKEL 180
           YGDHWR LRR+ A+EI S++RLNT  ++RRDE+RRL+ KLS+ SR  F +VE KSM+ EL
Sbjct: 121 YGDHWRNLRRIGAIEIFSSSRLNTTISVRRDEIRRLLLKLSRDSRQHFTKVELKSMLSEL 180

Query: 181 TLNITMRMVAGKRYYGEETDESSEAMTFRAIVDELFEFMLAQYPADFLPILKYIDFQGFM 240
           T N  MRMVAGKRYYG++    +EA  F  ++ E+F+   A  PADF P+L +I  Q + 
Sbjct: 181 TFNNIMRMVAGKRYYGDQVTNEAEAKEFWDLIAEVFKNGGAANPADFFPMLNWIG-QWYE 240

Query: 241 KRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLIL 300
           K  KKL +R+D F+Q ++DEHR+++  N     SM+AH L LQES+P YYTD+I+KGL+L
Sbjct: 241 KMDKKLGQRMDVFFQKLIDEHRSSRQEN----TSMIAHLLSLQESEPHYYTDEILKGLML 296

[Close]

BLAST of Cp4.1LG17g09460.1 vs. TAIR10
Match: AT4G37310.1 (AT4G37310.1 cytochrome P450, family 81, subfamily H, polypeptide 1)

HSP 1 Score: 272.3 bits (695), Expect = 3.7e-73
Identity = 144/291 (49.48%), Postives = 201/291 (69.07%), Query Frame = 1


Query: 13  IALLVAISYALKPKSRKNLPPTPP-SIPIIGHLHLLKPPV-HRSLLTLSQKYGPVLSLRF 72
           +A+ +     L  K  KNLPP+P    PIIGHLHLLK P+ HR+L  LS   GPV SLR 
Sbjct: 12  VAIFIVKIVLLVTKPNKNLPPSPNICFPIIGHLHLLKKPLLHRTLSHLSHSLGPVFSLRL 71

Query: 73  GSRLVVVVSSIDAAEECF-TKNDVVFANRPDFAVSKCLSYNHTTLGAAPYGDHWRKLRRV 132
           GSRL V++SS  AAEECF TKND+V ANRP F + K ++Y++T++  APYGDHWR LRR+
Sbjct: 72  GSRLAVIISSPTAAEECFLTKNDIVLANRPRFIMGKYVAYDYTSMVTAPYGDHWRNLRRI 131

Query: 133 SALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMVKELTLNITMRMVAG 192
           +ALE+ S NRLN ++ IR DEV+ L+ KL   S +  A+VE + ++  LTLN+ MRM+ G
Sbjct: 132 TALEVFSTNRLNASAEIRHDEVKMLLQKLHDLSVERPAKVELRQLLTGLTLNVIMRMMTG 191

Query: 193 KRYYGEET-DESSEAMTFRAIVDELFEFMLAQYPADFLPILKYIDFQGFMKRAKKLIKRV 252
           KR++ E+   ++  ++ FR +V E+ E   A  PADFLP L++ D++G +KRAK++ +R+
Sbjct: 192 KRFFEEDDGGKAGISLEFRELVAEILELSAADNPADFLPALRWFDYKGLVKRAKRIGERM 251

Query: 253 DAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILV 300
           D+  QG +DEHR N+    E +N+M+AH L  QE +P  Y+D  IKGLIL+
Sbjct: 252 DSLLQGFLDEHRANK-DRLEFKNTMIAHLLDSQEKEPHNYSDQTIKGLILM 301

[Close]

BLAST of Cp4.1LG17g09460.1 vs. TAIR10
Match: AT2G23220.1 (AT2G23220.1 cytochrome P450, family 81, subfamily D, polypeptide 6)

HSP 1 Score: 270.8 bits (691), Expect = 1.1e-72
Identity = 145/301 (48.17%), Postives = 203/301 (67.44%), Query Frame = 1


Query: 3   DTTFLYSVLGIALLVAISYAL-KPKSRKNLPPTPPS-IPIIGHLHLLKPPVHRSLLTLSQ 62
           +T FL   L   L +++     K  S+ NLPP+P   +P+IGHLHLLK P+HR+ L+ SQ
Sbjct: 17  ETYFLLLSLAFFLFISVKLLFGKRLSKFNLPPSPARPLPLIGHLHLLKLPLHRTFLSFSQ 76

Query: 63  KYG--PVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAP 122
             G  P+  LR G+RL VVVSS   AEECFTKND+VFANRP+  + K + YN TT+ +AP
Sbjct: 77  SLGGAPIFCLRLGNRLTVVVSSYSIAEECFTKNDIVFANRPELILGKHIEYNSTTMTSAP 136

Query: 123 YGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMVKEL 182
           YGDHWR LRR++ LEI ++++LN   ++R+DE+R+L+ +LS++SR  FA+VE + ++ EL
Sbjct: 137 YGDHWRNLRRIATLEIFASHKLNGFLSVRKDEIRQLLLRLSKNSRHGFAKVEMRHLLFEL 196

Query: 183 TLNITMRMVAGKRYYGEETDESSEAMTFRAIVDELFEFMLAQYPADFLPILKYIDFQGFM 242
           T+N   RMVAGKR+YGE T++   A   R ++DE+     A   AD+LPI+++  F  F 
Sbjct: 197 TINNVFRMVAGKRFYGEGTEQDEVAQQVRHLMDEIVTSAGAGNAADYLPIMRW--FTNFE 256

Query: 243 KRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLIL 300
           KR K L  R+D F Q +VDE R     ++E   +M+ H L LQESQP YYTD  +KG+I+
Sbjct: 257 KRVKNLAIRIDKFLQSLVDEKRA----DKEKGTTMIDHLLSLQESQPDYYTDVTLKGIII 311

[Close]

BLAST of Cp4.1LG17g09460.1 vs. TAIR10
Match: AT4G37370.1 (AT4G37370.1 cytochrome P450, family 81, subfamily D, polypeptide 8)

HSP 1 Score: 268.1 bits (684), Expect = 7.0e-72
Identity = 147/302 (48.68%), Postives = 207/302 (68.54%), Query Frame = 1


Query: 1   MEDTTFLYSVLGIALLVAISYAL-KPKSRKNLPPTPP-SIPIIGHLHLLKPPVHRSLLTL 60
           ME  T ++S+L + L  ++ Y + K K + NLPP+P  S+P+IGHL LLKPP+HR+ L+L
Sbjct: 1   METKTLIFSILFVVL--SLIYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSL 60

Query: 61  SQKYG--PVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGA 120
           SQ     P+ SLR G+RLV V SS   AEECFTKNDVV ANRP+F ++K ++Y++TT+ A
Sbjct: 61  SQSLNNAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIA 120

Query: 121 APYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMVK 180
           A YGDHWR LRR+ ++EI S +RLN+  +IR+DE+RRLV +LS++   +F +V+ KSM+ 
Sbjct: 121 ASYGDHWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLS 180

Query: 181 ELTLNITMRMVAGKRYYGEETDESSEAMTFRAIVDELFEFMLAQYPADFLPILKYIDFQG 240
           +LT N  +RMVAGKRYYG+  ++  EA   R ++ ++     A    D+LP+L+ +    
Sbjct: 181 DLTFNNILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SD 240

Query: 241 FMKRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGL 299
           +  R KKL  R+D F QG+VDE R      +E  N+M+ H L LQESQP Y+TD IIKG 
Sbjct: 241 YETRVKKLAGRLDEFLQGLVDEKR----EAKEKGNTMIDHLLTLQESQPDYFTDRIIKGN 294

[Close]

BLAST of Cp4.1LG17g09460.1 vs. TAIR10
Match: AT4G37340.1 (AT4G37340.1 cytochrome P450, family 81, subfamily D, polypeptide 3)

HSP 1 Score: 266.9 bits (681), Expect = 1.6e-71
Identity = 151/301 (50.17%), Postives = 204/301 (67.77%), Query Frame = 1


Query: 1   MEDTTFLYSVLGIALLVAISYALKPKSRKNLPPTPP-SIPIIGHLHLLKPPVHRSLLTLS 60
           ME  T +++ L I+L +      + K R NLPP+P  ++P+IGHL LLKPP+HR  L++S
Sbjct: 1   MEGQTLIFTFLFISLSLTFIIG-RIKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVS 60

Query: 61  QKYG--PVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAA 120
           +  G  P++SLR G+RLV VVSS   AEECFTKNDVV ANR +   SK +SY  TT+  A
Sbjct: 61  ESLGDAPIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTA 120

Query: 121 PYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMVKE 180
            YGDHWR LRR+ A+EI SA+RLN+ S+IRRDE+ RL+  LS++S  +F +VE KSM   
Sbjct: 121 SYGDHWRNLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSN 180

Query: 181 LTLNITMRMVAGKRYYGEETDESSEAMTFRAIVDELFEFMLAQYPADFLPILKYIDFQGF 240
           LT N  +RM+AGK YYG+  ++  EA   R ++ E      A   AD+LPIL +I   G 
Sbjct: 181 LTFNNIIRMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWIT--GS 240

Query: 241 MKRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLI 299
            KR KK+  R+D F QG+VDE R  +   ++ +N+MV H L LQE+QP+YYTD+IIKG++
Sbjct: 241 EKRIKKIASRLDEFLQGLVDERREGK---EKRQNTMVDHLLCLQETQPEYYTDNIIKGIM 295

[Close]

BLAST of Cp4.1LG17g09460.1 vs. TAIR10
Match: AT2G23190.1 (AT2G23190.1 cytochrome P450, family 81, subfamily D, polypeptide 7)

HSP 1 Score: 264.2 bits (674), Expect = 1.0e-70
Identity = 147/303 (48.51%), Postives = 202/303 (66.67%), Query Frame = 1


Query: 3   DTTFLYSVLGIALLVAISYAL---KPKSRKNLPPTPPS-IPIIGHLHLLKPPVHRSLLTL 62
           +T FL  +L +A L  IS  L   K  S+ NLPP+P   +P IGHLHLLK P+HR+ L+ 
Sbjct: 46  ETHFL--ILSLAFLFFISLKLLFGKRHSKFNLPPSPARPLPFIGHLHLLKQPLHRTFLSF 105

Query: 63  SQKYG--PVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGA 122
           SQ  G  P+ SLR G+ L VVVSS   AEECFTKND+V ANRP F + K + YN TT+ +
Sbjct: 106 SQSLGDAPIFSLRLGNHLTVVVSSYSIAEECFTKNDIVLANRPKFILGKHIEYNFTTMTS 165

Query: 123 APYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMVK 182
           APYGDHWR LRR+  LEI S+++LN   ++R+DE+R L+ +LS++S+  FA+VE + +  
Sbjct: 166 APYGDHWRNLRRIGTLEIFSSHKLNGFLSVRKDEIRHLLLRLSKNSQHGFAKVEMRQLFY 225

Query: 183 ELTLNITMRMVAGKRYYGEETDESSEAMTFRAIVDELFEFMLAQYPADFLPILKYIDFQG 242
           +LT+N  +RMVAGKR+YGE T++   A     ++DE+         AD++PIL++I    
Sbjct: 226 DLTINNILRMVAGKRFYGEGTEQDEVARRVTQLIDEIVYRAGVGNAADYIPILRWIT--D 285

Query: 243 FMKRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGL 300
           F K  K+L  RVD F Q +VDE R   +H Q+  N+M+ H L LQE+QP YYTD  +KG+
Sbjct: 286 FEKGVKELASRVDEFLQSLVDERR---VHKQK-GNTMMDHLLSLQETQPDYYTDVTLKGI 340

[Close]

BLAST of Cp4.1LG17g09460.1 vs. NCBI nr
Match: gi|659119971|ref|XP_008459942.1| (PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis melo])

HSP 1 Score: 455.7 bits (1171), Expect = 6.7e-125
Identity = 230/303 (75.91%), Postives = 257/303 (84.82%), Query Frame = 1


Query: 1   MEDTTFLYSV-----LGIALLVAISYALKPKSRKNLPPTPPSIPIIGHLHLLKPPVHRSL 60
           MED+   YS      +G+ +L  I Y LK  S KNLPP+PPSIPIIGHLHLLK PVHR+L
Sbjct: 1   MEDSILQYSAFSILSIGVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 60

Query: 61  LTLSQKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLG 120
            TLSQKYGPVLSLRFGSRLVVVV+S++AAEECFTKNDV+FANRP+FAVS CLSYN TTLG
Sbjct: 61  QTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLG 120

Query: 121 AAPYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMV 180
           AAPYGDHWRKLRRVS LEIL ++RLN N  IRRDEVRR V K+ + SRD F +VEFK +V
Sbjct: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAVKKIFEVSRDGFGKVEFKPLV 180

Query: 181 KELTLNITMRMVAGKRYYGEETDESSEAMTFRAIVDELFEFMLAQYPADFLPILKYIDFQ 240
           KELT+NITMRMVAGKRYYGEE  +SSEA TF+AIV ELFEF L+ YPADFLPILKYID Q
Sbjct: 181 KELTMNITMRMVAGKRYYGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 240

Query: 241 GFMKRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKG 299
           GFMKRAKKLI RVDAFWQG++DEHR  +I N+EM+N MVAHFLKLQE+QP YYTDDIIKG
Sbjct: 241 GFMKRAKKLIARVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 300

[Close]

BLAST of Cp4.1LG17g09460.1 vs. NCBI nr
Match: gi|701461490|ref|NP_001289568.1| (isoflavone 2'-hydroxylase-like [Cucumis sativus])

HSP 1 Score: 445.3 bits (1144), Expect = 9.0e-122
Identity = 223/303 (73.60%), Postives = 254/303 (83.83%), Query Frame = 1


Query: 1   MEDTTFLYSV-----LGIALLVAISYALKPKSRKNLPPTPPSIPIIGHLHLLKPPVHRSL 60
           MED+   YS      + + +L  I Y LK  S KNLPP+PPSIPIIGHLHLLK PVHR+L
Sbjct: 1   MEDSILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 60

Query: 61  LTLSQKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLG 120
            TLSQKYGPVLSLRFGSR VVVV+S++AAEECF KND++FANRP+FAVS CLSYN TTLG
Sbjct: 61  QTLSQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLG 120

Query: 121 AAPYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMV 180
           AAPYGDHWRKLRRVS LEIL ++RLN N  IRRDEVRR + K+ + SRD F +VEFK +V
Sbjct: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLV 180

Query: 181 KELTLNITMRMVAGKRYYGEETDESSEAMTFRAIVDELFEFMLAQYPADFLPILKYIDFQ 240
           KELT+N+TMRMVAGKRY+GEE  +SSEA TF+AIV ELFEF L+ YPADFLPILKYID Q
Sbjct: 181 KELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 240

Query: 241 GFMKRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKG 299
           GFMKRAKKLI RVDAFWQG++DEHR  +I N+EM+N MVAHFLKLQE+QP YYTDDIIKG
Sbjct: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 300

[Close]

BLAST of Cp4.1LG17g09460.1 vs. NCBI nr
Match: gi|590705372|ref|XP_007047419.1| (Cytochrome P450, family 81, subfamily D, polypeptide 5, putative [Theobroma cacao])

HSP 1 Score: 339.7 bits (870), Expect = 5.4e-90
Identity = 164/299 (54.85%), Postives = 223/299 (74.58%), Query Frame = 1


Query: 1   MEDTTFLYSVLGIALLVAISYALKPKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLLTLSQ 60
           ME T        + LLVAI + L+ +  KNLPP+P ++PI+GHLHLLK P+HR+L TLSQ
Sbjct: 30  MEQTLLCSLASVLFLLVAIRFLLQARKHKNLPPSPFALPILGHLHLLKEPLHRTLFTLSQ 89

Query: 61  KYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPYG 120
           K+GP+ SLRFGSRL VV++S  A EECF KND+  ANRP   + K + YN+T L  A YG
Sbjct: 90  KHGPIFSLRFGSRLAVVITSPSAVEECFNKNDITLANRPRLIMGKYVGYNYTILALASYG 149

Query: 121 DHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMVKELTL 180
           DHWR LRR++ LEILS+N L+ +S+IRRDE+  L+ +L + S + FA+VE K++  ELT 
Sbjct: 150 DHWRNLRRLATLEILSSNNLSMSSSIRRDEINSLIRRLYRVSVNGFAKVELKTLFAELTF 209

Query: 181 NITMRMVAGKRYYGEETDESSEAMTFRAIVDELFEFMLAQYPADFLPILKYIDFQGFMKR 240
           NI MR+VAGKRYYG+E   S EA  FRA+++ELFE  ++ YP DFLP+L ++D+ G++K+
Sbjct: 210 NIIMRIVAGKRYYGDEVRGSEEARGFRAMIEELFELTVSSYPGDFLPLLSWVDYNGYIKK 269

Query: 241 AKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILV 300
            K + K  DAF QG++DEHR N+  N E++N+M++H L LQ+SQP+YYTD+IIKGL+LV
Sbjct: 270 VKLVAKNADAFTQGLIDEHRRNK-GNLELKNTMISHLLTLQKSQPEYYTDEIIKGLVLV 327

[Close]

BLAST of Cp4.1LG17g09460.1 vs. NCBI nr
Match: gi|590651870|ref|XP_007033004.1| (Cytochrome P450, family 81, subfamily D, polypeptide 8, putative [Theobroma cacao])

HSP 1 Score: 335.1 bits (858), Expect = 1.3e-88
Identity = 173/301 (57.48%), Postives = 224/301 (74.42%), Query Frame = 1


Query: 1   MEDTTFLYSVLGIALL-VAISYALKPK-SRKNLPPTPPSIPIIGHLHLLKPPVHRSLLTL 60
           ME+TT LYS L + LL +++++  + K S KNLPP+PPS+PI+GHLHLLKP +HRS  TL
Sbjct: 1   MEETTILYSSLSLVLLLISLNFFFQSKKSHKNLPPSPPSLPILGHLHLLKPLIHRSYHTL 60

Query: 61  SQKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAP 120
           SQKYGP+ S++ GSRL VVVSS  AAEECFTKND++ ANRP     K L YN+TTL A+P
Sbjct: 61  SQKYGPIFSIQLGSRLQVVVSSSAAAEECFTKNDIILANRPKLIQGKHLGYNYTTLIASP 120

Query: 121 YGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMVKEL 180
           YGDHWR LRR+ A+EI S++RLNT+ ++RRDE+RRL+ KLS+ SR  F +VE KSM+ EL
Sbjct: 121 YGDHWRNLRRIGAIEIFSSSRLNTSISVRRDEIRRLLLKLSRDSRQHFTKVELKSMLSEL 180

Query: 181 TLNITMRMVAGKRYYGEETDESSEAMTFRAIVDELFEFMLAQYPADFLPILKYIDFQGFM 240
           T N  MRMVAGKRYYG+E    +EA  FR ++ E+ +   A  PADF P+L +   Q + 
Sbjct: 181 TFNNIMRMVAGKRYYGDEVTNEAEAKEFRDLIAEVVKHGGAANPADFFPMLSWFG-QWYE 240

Query: 241 KRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLIL 300
           K+ KKL KR+D F+Q ++DEHR+ +  N     SM+AH L LQES+P YYTDDI+KGL+L
Sbjct: 241 KKDKKLGKRMDVFFQKLIDEHRSYRQEN----TSMIAHLLSLQESEPHYYTDDILKGLVL 296

[Close]

BLAST of Cp4.1LG17g09460.1 vs. NCBI nr
Match: gi|802644603|ref|XP_012079326.1| (PREDICTED: isoflavone 3'-hydroxylase-like [Jatropha curcas])

HSP 1 Score: 332.4 bits (851), Expect = 8.5e-88
Identity = 171/300 (57.00%), Postives = 219/300 (73.00%), Query Frame = 1


Query: 1   MEDTTFLY-SVLGIALLVAISYALKPKSRKNLPPTPPSIPIIGHLHLLKPPVHRSLLTLS 60
           ME  T LY S   + L + I +      RKNLPP+PPS PIIGHL+LLKPP+HR L +L+
Sbjct: 1   MEHYTVLYFSSSFLLLFLVIKFCQSKVHRKNLPPSPPSFPIIGHLYLLKPPMHRILHSLA 60

Query: 61  QKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLGAAPY 120
           Q YGPV+ LRFGSR +++VSS+  AEECFTKND+VFANRP   ++K +SYN+TT+  APY
Sbjct: 61  QTYGPVIFLRFGSRPLIIVSSLSIAEECFTKNDIVFANRPRLLMAKHISYNYTTMTQAPY 120

Query: 121 GDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMVKELT 180
           GDHWR LRRV A+EI SA+RLNT  +IR+DE++RL+ KLS +S  DF +VE KSM +ELT
Sbjct: 121 GDHWRNLRRVGAIEIFSAHRLNTLLSIRKDEIKRLIMKLSINSMQDFCKVELKSMFQELT 180

Query: 181 LNITMRMVAGKRYYGEETDESSEAMTFRAIVDELFEFMLAQYPADFLPILKYIDFQGFMK 240
            NI +RMVAGKRYYG++     EA  FR ++ ++  +  A  PADFLPIL +ID     K
Sbjct: 181 FNIMIRMVAGKRYYGDDVSNEEEAREFRKLMKKVVAYAGASNPADFLPILNWIDGGYLRK 240

Query: 241 RAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKGLILV 300
           R K   KR D F+QG++DEHR+ +  N E +N+M+ H L LQESQP+YYTD+IIKGLI V
Sbjct: 241 RIKGFAKRFDNFFQGLIDEHRSKK-GNLENKNTMIDHLLSLQESQPEYYTDEIIKGLIQV 299

The following BLAST results are available for this feature:

BLAST of Cp4.1LG17g09460.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
C81E1_GLYEC	3.3e-79	51.19	Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2	[more]
C81E9_MEDTR	2.8e-78	48.76	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula GN=CYP81E9 PE=1 SV=1	[more]
C81E8_MEDTR	1.4e-77	51.33	Cytochrome P450 81E8 OS=Medicago truncatula GN=CYP81E8 PE=2 SV=1	[more]
C81E7_MEDTR	1.2e-73	48.46	Isoflavone 2'-hydroxylase OS=Medicago truncatula GN=CYP81E7 PE=1 SV=1	[more]
C8D11_ARATH	3.2e-66	47.52	Cytochrome P450 81D11 OS=Arabidopsis thaliana GN=CYP81D11 PE=2 SV=1	[more]

BLAST of Cp4.1LG17g09460.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A097IYP1_CUCSA	6.3e-122	73.60	Cytochrome P450 OS=Cucumis sativus GN=Csa6G088160 PE=2 SV=1	[more]
A0A061DI90_THECC	3.7e-90	54.85	Cytochrome P450, family 81, subfamily D, polypeptide 5, putative OS=Theobroma ca...	[more]
A0A061EFX9_THECC	9.2e-89	57.48	Cytochrome P450, family 81, subfamily D, polypeptide 8, putative OS=Theobroma ca...	[more]
A0A067KJK1_JATCU	6.0e-88	57.00	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_12475 PE=3 SV=1	[more]
A0A061EGZ3_THECC	2.3e-87	56.81	Cytochrome P450, family 81, subfamily D, polypeptide 8 OS=Theobroma cacao GN=TCM...	[more]

BLAST of Cp4.1LG17g09460.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G37310.1	3.7e-73	49.48	cytochrome P450, family 81, subfamily H, polypeptide 1	[more]
AT2G23220.1	1.1e-72	48.17	cytochrome P450, family 81, subfamily D, polypeptide 6	[more]
AT4G37370.1	7.0e-72	48.68	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]
AT4G37340.1	1.6e-71	50.17	cytochrome P450, family 81, subfamily D, polypeptide 3	[more]
AT2G23190.1	1.0e-70	48.51	cytochrome P450, family 81, subfamily D, polypeptide 7	[more]

BLAST of Cp4.1LG17g09460.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659119971\|ref\|XP_008459942.1\|	6.7e-125	75.91	PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis melo]	[more]
gi\|701461490\|ref\|NP_001289568.1\|	9.0e-122	73.60	isoflavone 2'-hydroxylase-like [Cucumis sativus]	[more]
gi\|590705372\|ref\|XP_007047419.1\|	5.4e-90	54.85	Cytochrome P450, family 81, subfamily D, polypeptide 5, putative [Theobroma caca...	[more]
gi\|590651870\|ref\|XP_007033004.1\|	1.3e-88	57.48	Cytochrome P450, family 81, subfamily D, polypeptide 8, putative [Theobroma caca...	[more]
gi\|802644603\|ref\|XP_012079326.1\|	8.5e-88	57.00	PREDICTED: isoflavone 3'-hydroxylase-like [Jatropha curcas]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0020037	heme binding
GO:0005506	iron ion binding
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Vocabulary: **INTERPRO**
Term	Definition
IPR002401	Cyt_P450_E_grp-I
IPR001128	Cyt_P450

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function	GO:0004497	monooxygenase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cp4.1LG17g09460	Cp4.1LG17g09460	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG17g09460.1:cds:001	Cp4.1LG17g09460.1:cds:001	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG17g09460.1	Cp4.1LG17g09460.1-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	GENE3D	G3DSA:1.10.630.10		coord: 24..297 score: 7.5
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 32..295 score: 2.5
IPR001128	Cytochrome P450	unknown	SSF48264	Cytochrome P450	coord: 32..298 score: 1.96
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 59..78 score: 6.0E-13coord: 83..104 score: 6.0E-13coord: 178..196 score: 6.0E-13coord: 309..316 score: 6.0
None	No IPR available	unknown	Coil	Coil	coord: 312..316 scor
None	No IPR available	PANTHER	PTHR24298	FAMILY NOT NAMED	coord: 2..299 score: 1.8E
None	No IPR available	PANTHER	PTHR24298:SF116	SUBFAMILY NOT NAMED	coord: 2..299 score: 1.8E