Cp4.1LG16g01440.1 (mRNA) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG16g01440.1
Type	mRNA
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	Cytochrome P450, putative
Location	Cp4.1LG16 : 3352024 .. 3352996 (+)
Sequence length	546

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTAGGTTACAAAAACAATCAATTTTTACTTTTAAATTTACTAGCAAATTGTTAATAATACATTACGAGATGAAATAAACCGTAACACACATAACTGTCGTAATAAAATTCATCAAGCTAAGATTTCCCAAAAACTTTCGCGTATTTGTATTAATGGAAATTAAATGGAAACTCAATTTTCTCCTCTTCATTTACTCTATATATTCATAGTTGAAATGAGATGATAACCAACAAAGCAAAGAAAAAAAACATGGAAAACCTTATGCTTTGTCTCTGTCTCCCTCTAATGGCATTCATATCAGCTCTCATATTTCTACTCCGACCCTCCACAAAGCTTCCCCCAAGTCCCCCTTTTTCCCTCCCAATTATAGGCCATCTCTATCTCCTAAAGCGTCCCGTCCATCAAACCCTTCAAAAGCTTTCTAACAAGTATGGCCATGTCTTCACTCTCCGCTTAGGCTCTCAACTCGTTGTCGTTGTCTCGTCTCCTTCGGTGGTTAAAGAATGCTTCACAATGAATGATATCATTCTAGCAAATCGACCAAGCTCGAACATTATCAAGCACTTGACTTACAACAACACGATCTTTTCGGCGTCCCCATATGGCGATCATTGGCGCGAACTCCGCCGAATTAGCACCATAGAGATATTCTCAGCCACTCGCCTTAATATGTTCTTGGAGACAAGGAAGGACGAAATCAAACACCTACTTGGTAAATTGTGTTGCATTTCAAGTGATAGCTTTGTGAGAGTTGAGATGAGTTCCATGATTTCTGAGTTGTCGTCAAATATTGTGATGAGAATGGTGATGGGGAAGAGGTTTTATGGAGAGGGTGTGTCGGATTTGGAACAAGCGAGGAAATTTAGAGATATTGTGAAGCAAGTTATGCAAAAATGTGGGAGTTTTGATTCAAGAAGTTTTATTCCATTGTTGAATTGGATAGATATTACTCATTATGAGAAGAGGATAA

mRNA sequence

ATGTTAGGCCATCTCTATCTCCTAAAGCGTCCCGTCCATCAAACCCTTCAAAAGCTTTCTAACAAGTATGGCCATGTCTTCACTCTCCGCTTAGGCTCTCAACTCGTTGTCGTTGTCTCGTCTCCTTCGGTGGTTAAAGAATGCTTCACAATGAATGATATCATTCTAGCAAATCGACCAAGCTCGAACATTATCAAGCACTTGACTTACAACAACACGATCTTTTCGGCGTCCCCATATGGCGATCATTGGCGCGAACTCCGCCGAATTAGCACCATAGAGATATTCTCAGCCACTCGCCTTAATATGTTCTTGGAGACAAGGAAGGACGAAATCAAACACCTACTTGGTAAATTGTGTTGCATTTCAAGTGATAGCTTTGTGAGAGTTGAGATGAGTTCCATGATTTCTGAGTTGTCGTCAAATATTGTGATGAGAATGGTGATGGGGAAGAGGTTTTATGGAGAGGGTGTGTCGGATTTGGAACAAGCGAGGAAATTTAGAGATATTGTGAAGCAAATATTACTCATTATGAGAAGAGGATAA

Coding sequence (CDS)

ATGTTAGGCCATCTCTATCTCCTAAAGCGTCCCGTCCATCAAACCCTTCAAAAGCTTTCTAACAAGTATGGCCATGTCTTCACTCTCCGCTTAGGCTCTCAACTCGTTGTCGTTGTCTCGTCTCCTTCGGTGGTTAAAGAATGCTTCACAATGAATGATATCATTCTAGCAAATCGACCAAGCTCGAACATTATCAAGCACTTGACTTACAACAACACGATCTTTTCGGCGTCCCCATATGGCGATCATTGGCGCGAACTCCGCCGAATTAGCACCATAGAGATATTCTCAGCCACTCGCCTTAATATGTTCTTGGAGACAAGGAAGGACGAAATCAAACACCTACTTGGTAAATTGTGTTGCATTTCAAGTGATAGCTTTGTGAGAGTTGAGATGAGTTCCATGATTTCTGAGTTGTCGTCAAATATTGTGATGAGAATGGTGATGGGGAAGAGGTTTTATGGAGAGGGTGTGTCGGATTTGGAACAAGCGAGGAAATTTAGAGATATTGTGAAGCAAATATTACTCATTATGAGAAGAGGATAA

Protein sequence

MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRPSSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLCCISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQILLIMRRG

Homology

[Close]

BLAST of Cp4.1LG16g01440.1 vs. Swiss-Prot
Match: C81E8_MEDTR (Cytochrome P450 81E8 OS=Medicago truncatula GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 5.5e-47
Identity = 96/177 (54.24%), Postives = 128/177 (72.32%), Query Frame = 1


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP 60
           ++G+L+ LK+P+H T   LS KYG +F+L  GS+LVVVVSS ++ +ECFT NDI+LANRP
Sbjct: 41  IIGNLHQLKQPLHHTFHTLSQKYGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRP 100

Query: 61  SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLC 120
                K++ YNNT  + SPYGDHWR LRRI +IEI S+ RLN FLE R+DEI  L+ KL 
Sbjct: 101 HFLTGKYIGYNNTTVAQSPYGDHWRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLA 160

Query: 121 CISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGE--GVSDLEQARKFRDIVKQIL 176
             S + F  VE+  M SE++ N +MRMV GKR+YG    VSD+E+AR FR I+K+++
Sbjct: 161 QKSYNGFTEVELRPMFSEMTFNTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVV 217

[Close]

BLAST of Cp4.1LG16g01440.1 vs. Swiss-Prot
Match: C81E1_GLYEC (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 171.0 bits (432), Expect = 1.2e-41
Identity = 84/177 (47.46%), Postives = 127/177 (71.75%), Query Frame = 1


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP 60
           ++G+L+ LKRP+H+T + LS KYGHVF+L  GS+LVVVVSS S  ++CFT ND++LANRP
Sbjct: 41  IIGNLHHLKRPLHRTFKGLSEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRP 100

Query: 61  SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLC 120
                K++ YN T   ++ YG+HWR LRRI+ +++ S  R+N F   R+DE + L+ +L 
Sbjct: 101 RFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLA 160

Query: 121 CISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGE--GVSDLEQARKFRDIVKQIL 176
             SS +F  +E+SS + +++ N +MRM+ GKR+YGE    SDL++A +FRD+V ++L
Sbjct: 161 DDSSTNFAEMELSSRLYDMTFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELL 217

[Close]

BLAST of Cp4.1LG16g01440.1 vs. Swiss-Prot
Match: C81E9_MEDTR (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 2.6e-41
Identity = 86/183 (46.99%), Postives = 126/183 (68.85%), Query Frame = 1


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP 60
           ++G+L+ LK P+H+T   LS  YG +F+L  GS+LVVVVSSPS+  ECFT NDIILANRP
Sbjct: 39  IIGNLHHLKHPLHRTFTTLSQTYGDIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRP 98

Query: 61  SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKL- 120
                K++ YN T   ++ YGDHWR LRRI+TI++ S  RLN FL  R+DE   L+ KL 
Sbjct: 99  RFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLL 158

Query: 121 --CCISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGE--GVSDLEQARKFRDIVKQIL 179
                    F +VE+   ++E++ N +MRM+ GKR+YG+   VSD+E+A++FR+I+ +++
Sbjct: 159 KDVVSEGFGFTKVELRPRLTEMTFNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMM 218

[Close]

BLAST of Cp4.1LG16g01440.1 vs. Swiss-Prot
Match: C81D1_ARATH (Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.0e-40
Identity = 89/180 (49.44%), Postives = 121/180 (67.22%), Query Frame = 1


Query: 1   MLGHLYLLKRPVHQTLQKLS-----NKYGHVFTLRLGSQLVVVVSSPSVV-KECFTMNDI 60
           ++GHL LLK P+H+TL+  S     N  G V +LRLGS+LV VVSS  V  +ECF  ND+
Sbjct: 43  IIGHLRLLKPPIHRTLRSFSETLDHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDV 102

Query: 61  ILANRPSSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKH 120
           +LANRP   I KH+ YNNT   A+PYGDHWR LRR+ TIEIFS  RLN FL  R DE++ 
Sbjct: 103 VLANRPQVIIGKHVGYNNTNMIAAPYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRR 162

Query: 121 LLGKLCCISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQI 175
           L+ +L  ++      VE+  M+ +L+ N +MRM+ GKR+YGE  +D E+A++ R +V  +
Sbjct: 163 LISRLSRLAGTKKTVVELKPMLMDLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADV 222

[Close]

BLAST of Cp4.1LG16g01440.1 vs. Swiss-Prot
Match: C8D11_ARATH (Cytochrome P450 81D11 OS=Arabidopsis thaliana GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 3.2e-39
Identity = 83/178 (46.63%), Postives = 120/178 (67.42%), Query Frame = 1


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGH--VFTLRLGSQLVVVVSSPSVVKECFTMNDIILAN 60
           ++GHL+LLK P+H+    LS    +  +F+L LGS+LV VVSS +V +ECFT ND++LAN
Sbjct: 49  IIGHLHLLKLPLHRRFLSLSESLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLAN 108

Query: 61  RPSSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGK 120
           RP   + KH+ YN+T    + YGD WR LRRI TIEIFS+ RLN F+  R+DEI+ L+  
Sbjct: 109 RPEFLVGKHIGYNSTTMVGAAYGDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIIC 168

Query: 121 LCCISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQILL 177
           L   S   FV+VEM  +   L+ N ++RMV GKRFYG+G  +  +A+  R ++ ++++
Sbjct: 169 LAKNSQHGFVKVEMKPLFMCLTINNIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVV 226

[Close]

BLAST of Cp4.1LG16g01440.1 vs. TrEMBL
Match: M5WME3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa025296mg PE=3 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 3.0e-52
Identity = 105/175 (60.00%), Postives = 134/175 (76.57%), Query Frame = 1


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP 60
           +LGHL+LLK P+H+TLQ+LS K+GH+F+L  GSQ VV+VSS S  +ECFT NDI+LANRP
Sbjct: 40  ILGHLHLLKIPIHRTLQRLSQKHGHIFSLWFGSQRVVIVSSSSAAQECFTKNDIVLANRP 99

Query: 61  SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLC 120
            S + KHL YNNT    + YGDHWR LRRI  IEIFS +RLN FL  RKDE+KHLL KL 
Sbjct: 100 RSLVSKHLAYNNTTIGTAQYGDHWRNLRRIGAIEIFSTSRLNAFLNVRKDEVKHLLLKLS 159

Query: 121 CISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGE-GVSDLEQARKFRDIVKQI 175
             + D F +VE+ SM  +L+ N++MRMV GKR+YGE G  D E+AR+FR+I+++I
Sbjct: 160 QNARDDFGKVELKSMFLDLTLNVIMRMVAGKRYYGEDGSVDKEEARQFREIMEEI 214

[Close]

BLAST of Cp4.1LG16g01440.1 vs. TrEMBL
Match: M5WQ76_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa026692mg PE=3 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 5.1e-52
Identity = 104/175 (59.43%), Postives = 134/175 (76.57%), Query Frame = 1


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP 60
           +LGHL+LLK PVH+T  +LS K+G +F+L  GSQ VV+VSSPS V+ECFT NDI+LANRP
Sbjct: 39  ILGHLHLLKAPVHRTFHRLSQKHGPIFSLWFGSQRVVIVSSPSAVQECFTRNDIVLANRP 98

Query: 61  SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLC 120
           S  + KH+ YN+T  S SPYGDHWR LRRI  IEIFS+ RLN F  TRKDE++HL+ KL 
Sbjct: 99  SLLLFKHIGYNSTTISTSPYGDHWRNLRRIGAIEIFSSARLNTFANTRKDEVRHLICKLA 158

Query: 121 CISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVS-DLEQARKFRDIVKQI 175
             S   F +VE+ SM +EL+ NI+M MV GKR+YG+ VS D E+A++FR I+K++
Sbjct: 159 QNSVHEFAKVELKSMFTELTFNIIMTMVAGKRYYGDDVSVDKEEAKQFRQIMKEV 213

[Close]

BLAST of Cp4.1LG16g01440.1 vs. TrEMBL
Match: M5WJL2_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa017987mg PE=3 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 6.7e-52
Identity = 105/175 (60.00%), Postives = 133/175 (76.00%), Query Frame = 1


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP 60
           +LGHL+LLK PVH+T  +LS K+G +F+L  GSQ VV+VSSPS V+ECFT NDI+LANRP
Sbjct: 50  ILGHLHLLKAPVHRTFHRLSQKHGPIFSLWFGSQRVVIVSSPSAVQECFTRNDIVLANRP 109

Query: 61  SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLC 120
           S  + KH+ YN+T  S SPYGDHWR LRRI  IEIFS+ RLN F  TRKDE+KHL+ KL 
Sbjct: 110 SLLLFKHIGYNSTTISTSPYGDHWRNLRRIGAIEIFSSARLNTFANTRKDEVKHLICKLA 169

Query: 121 CISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVS-DLEQARKFRDIVKQI 175
             S   F +VE+ SM +EL+ NI+M MV GKR+YG  VS D E+A++FR I+K++
Sbjct: 170 QNSVHEFAKVELKSMFTELTFNIIMTMVAGKRYYGVDVSVDKEEAKQFRQIMKEV 224

[Close]

BLAST of Cp4.1LG16g01440.1 vs. TrEMBL
Match: W9R990_9ROSA (Cytochrome P450 81D1 OS=Morus notabilis GN=L484_009303 PE=3 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 1.1e-51
Identity = 109/176 (61.93%), Postives = 136/176 (77.27%), Query Frame = 1


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP 60
           ++GHL+LLK P+H+TL  LS KYG VF+LR GS+ +VV+SSPSVV+ECFT ND++LANRP
Sbjct: 41  VIGHLHLLKIPLHRTLLGLSQKYGPVFSLRFGSRRLVVLSSPSVVEECFTKNDVVLANRP 100

Query: 61  SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLC 120
              I KH+ YN+T  S +PYGDHWR LRRI+ IEIFSA RL MFL  R+DE K LL KL 
Sbjct: 101 PLLISKHIGYNSTTVSTAPYGDHWRNLRRIAAIEIFSAGRLKMFLGIRRDETKRLLTKLY 160

Query: 121 CIS---SDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQ 174
             S    D+F +VE+ SM SEL+ NI+MRMV GKR+YGEGV+D E+A +FR IVK+
Sbjct: 161 SHSQQAGDNFAKVELKSMFSELTFNIIMRMVAGKRYYGEGVTDEEEAVEFRKIVKE 216

[Close]

BLAST of Cp4.1LG16g01440.1 vs. TrEMBL
Match: A0A0A0LTD0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G425740 PE=3 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 4.4e-51
Identity = 106/180 (58.89%), Postives = 135/180 (75.00%), Query Frame = 1


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP 60
           ++GHL+LLK P+HQTL  LS KYGHVF+LR GS LVVV+SSPS V+ECFT NDIILANRP
Sbjct: 40  LIGHLHLLKHPIHQTLHSLSKKYGHVFSLRFGSHLVVVISSPSAVRECFTKNDIILANRP 99

Query: 61  SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLC 120
             N  KHL+YN T+ + +PYG+ WR LRRIST EIFS ++LN F   R+DE+K LL KLC
Sbjct: 100 FLNTGKHLSYNFTVLAVAPYGELWRRLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLC 159

Query: 121 CISSDS----FVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQILL 177
             +S      F  VE+  ++ + +SNIVMRMV GKR++G+ V D  QA KFRD+VK+++L
Sbjct: 160 NRNSVEELLLFSAVELEPILLDFTSNIVMRMVGGKRYFGDDVLDEGQAEKFRDVVKRVML 219

[Close]

BLAST of Cp4.1LG16g01440.1 vs. TAIR10
Match: AT2G23190.1 (AT2G23190.1 cytochrome P450, family 81, subfamily D, polypeptide 7)

HSP 1 Score: 182.2 bits (461), Expect = 2.9e-46
Identity = 91/176 (51.70%), Postives = 123/176 (69.89%), Query Frame = 1


Query: 2   LGHLYLLKRPVHQTLQKLSNKYGH--VFTLRLGSQLVVVVSSPSVVKECFTMNDIILANR 61
           +GHL+LLK+P+H+T    S   G   +F+LRLG+ L VVVSS S+ +ECFT NDI+LANR
Sbjct: 86  IGHLHLLKQPLHRTFLSFSQSLGDAPIFSLRLGNHLTVVVSSYSIAEECFTKNDIVLANR 145

Query: 62  PSSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKL 121
           P   + KH+ YN T  +++PYGDHWR LRRI T+EIFS+ +LN FL  RKDEI+HLL +L
Sbjct: 146 PKFILGKHIEYNFTTMTSAPYGDHWRNLRRIGTLEIFSSHKLNGFLSVRKDEIRHLLLRL 205

Query: 122 CCISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQIL 176
              S   F +VEM  +  +L+ N ++RMV GKRFYGEG    E AR+   ++ +I+
Sbjct: 206 SKNSQHGFAKVEMRQLFYDLTINNILRMVAGKRFYGEGTEQDEVARRVTQLIDEIV 261

[Close]

BLAST of Cp4.1LG16g01440.1 vs. TAIR10
Match: AT2G23220.1 (AT2G23220.1 cytochrome P450, family 81, subfamily D, polypeptide 6)

HSP 1 Score: 176.8 bits (447), Expect = 1.2e-44
Identity = 90/177 (50.85%), Postives = 124/177 (70.06%), Query Frame = 1


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGH--VFTLRLGSQLVVVVSSPSVVKECFTMNDIILAN 60
           ++GHL+LLK P+H+T    S   G   +F LRLG++L VVVSS S+ +ECFT NDI+ AN
Sbjct: 56  LIGHLHLLKLPLHRTFLSFSQSLGGAPIFCLRLGNRLTVVVSSYSIAEECFTKNDIVFAN 115

Query: 61  RPSSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGK 120
           RP   + KH+ YN+T  +++PYGDHWR LRRI+T+EIF++ +LN FL  RKDEI+ LL +
Sbjct: 116 RPELILGKHIEYNSTTMTSAPYGDHWRNLRRIATLEIFASHKLNGFLSVRKDEIRQLLLR 175

Query: 121 LCCISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQIL 176
           L   S   F +VEM  ++ EL+ N V RMV GKRFYGEG    E A++ R ++ +I+
Sbjct: 176 LSKNSRHGFAKVEMRHLLFELTINNVFRMVAGKRFYGEGTEQDEVAQQVRHLMDEIV 232

[Close]

BLAST of Cp4.1LG16g01440.1 vs. TAIR10
Match: AT4G37370.1 (AT4G37370.1 cytochrome P450, family 81, subfamily D, polypeptide 8)

HSP 1 Score: 176.8 bits (447), Expect = 1.2e-44
Identity = 87/177 (49.15%), Postives = 126/177 (71.19%), Query Frame = 1


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGH--VFTLRLGSQLVVVVSSPSVVKECFTMNDIILAN 60
           ++GHL LLK P+H+T   LS    +  +F+LRLG++LV V SS S+ +ECFT ND++LAN
Sbjct: 40  VIGHLRLLKPPIHRTFLSLSQSLNNAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLAN 99

Query: 61  RPSSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGK 120
           RP+  + KH+ Y+ T   A+ YGDHWR LRRI ++EIFS  RLN FL  RKDEI+ L+ +
Sbjct: 100 RPNFILAKHVAYDYTTMIAASYGDHWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFR 159

Query: 121 LCCISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQIL 176
           L    S  FV+V+M SM+S+L+ N ++RMV GKR+YG+GV D  +A++ R ++  ++
Sbjct: 160 LSRNFSQEFVKVDMKSMLSDLTFNNILRMVAGKRYYGDGVEDDPEAKRVRQLIADVV 216

[Close]

BLAST of Cp4.1LG16g01440.1 vs. TAIR10
Match: AT4G37320.1 (AT4G37320.1 cytochrome P450, family 81, subfamily D, polypeptide 5)

HSP 1 Score: 171.0 bits (432), Expect = 6.6e-43
Identity = 83/177 (46.89%), Postives = 124/177 (70.06%), Query Frame = 1


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGH--VFTLRLGSQLVVVVSSPSVVKECFTMNDIILAN 60
           ++GHL+LLK+PVH+T   +S   G+  +F LRLG++LV V+SS S+ +ECFT ND++LAN
Sbjct: 41  VIGHLHLLKQPVHRTFHSISKSLGNAPIFHLRLGNRLVYVISSHSIAEECFTKNDVVLAN 100

Query: 61  RPSSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGK 120
           RP   + KH+ YN T   A+ YGDHWR LRRI+ +EIFS+ R++ F   RKDEI+ L+  
Sbjct: 101 RPDIIMAKHVGYNFTNMIAASYGDHWRNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITH 160

Query: 121 LCCISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQIL 176
           L   S   FV VE+ S+++ L+ N ++ MV GKR+YG G  D ++A+  R+++ +I+
Sbjct: 161 LSRDSLHGFVEVELKSLLTNLAFNNIIMMVAGKRYYGTGTEDNDEAKLVRELIAEIM 217

[Close]

BLAST of Cp4.1LG16g01440.1 vs. TAIR10
Match: AT4G37330.1 (AT4G37330.1 cytochrome P450, family 81, subfamily D, polypeptide 4)

HSP 1 Score: 169.1 bits (427), Expect = 2.5e-42
Identity = 87/175 (49.71%), Postives = 121/175 (69.14%), Query Frame = 1


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGH--VFTLRLGSQLVVVVSSPSVVKECFTMNDIILAN 60
           ++GHL+LLK P+H+T   LS   G+  VF LRLG++LV V+SS S+ +ECFT ND++LAN
Sbjct: 41  VIGHLHLLKPPLHRTFLSLSKSIGNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLAN 100

Query: 61  RPSSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGK 120
           RP   I KHL YN T   ++ YGDHWR LRRI+ +EIFS  RLN FL  RKDEI+ L+  
Sbjct: 101 RPKFTISKHLGYNATYLLSASYGDHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISH 160

Query: 121 LCCISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQ 174
           L   S   FV VEM ++++ L+SN  +RM+ GKR++GE   D + A+  +++V +
Sbjct: 161 LSRDSLHGFVEVEMKTLLTNLASNTTIRMLAGKRYFGE---DNDDAKLVKNLVSE 212

[Close]

BLAST of Cp4.1LG16g01440.1 vs. NCBI nr
Match: gi|1009174377|ref|XP_015868316.1| (PREDICTED: cytochrome P450 81E8-like isoform X1 [Ziziphus jujuba])

HSP 1 Score: 221.5 bits (563), Expect = 1.2e-54
Identity = 110/174 (63.22%), Postives = 141/174 (81.03%), Query Frame = 1


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP 60
           ++GHL+LLK PVH+T  +LS KYG +F+L LGS+ VVVVSS S V ECFT NDI+LA+RP
Sbjct: 41  IVGHLHLLKHPVHRTYHRLSQKYGPIFSLWLGSRRVVVVSSASAVHECFTKNDIVLASRP 100

Query: 61  SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLC 120
           + +I KHLTYN+T+ + +PYGDHWR LRRI +IEIFS++RLNMFL  RKDE+K LL KL 
Sbjct: 101 ALHIRKHLTYNSTVMTVAPYGDHWRNLRRIGSIEIFSSSRLNMFLGIRKDEVKRLLLKLS 160

Query: 121 CISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQI 175
             S   F RVEM S++SEL+ NI+MRMV GKR+YG+ VSD E+AR+FR+I+K+I
Sbjct: 161 QNSVQDFKRVEMKSLLSELTFNIIMRMVSGKRYYGDEVSDEEEARQFREIMKEI 214

[Close]

BLAST of Cp4.1LG16g01440.1 vs. NCBI nr
Match: gi|1009174371|ref|XP_015868313.1| (PREDICTED: cytochrome P450 81E8-like [Ziziphus jujuba])

HSP 1 Score: 221.5 bits (563), Expect = 1.2e-54
Identity = 110/174 (63.22%), Postives = 141/174 (81.03%), Query Frame = 1


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP 60
           ++GHL+LLK PVH+T  +LS KYG +F+L LGS+ VVVVSS S V ECFT NDI+LA+RP
Sbjct: 41  IVGHLHLLKHPVHRTYHRLSQKYGPIFSLWLGSRRVVVVSSASAVHECFTKNDIVLASRP 100

Query: 61  SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLC 120
           + +I KHLTYN+T+ + +PYGDHWR LRRI +IEIFS++RLNMFL  RKDE+K LL KL 
Sbjct: 101 ALHIRKHLTYNSTVMTVAPYGDHWRNLRRIGSIEIFSSSRLNMFLGIRKDEVKRLLLKLS 160

Query: 121 CISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQI 175
             S   F RVEM S++SEL+ NI+MRMV GKR+YG+ VSD E+AR+FR+I+K+I
Sbjct: 161 QNSVQDFKRVEMKSLLSELTFNIIMRMVSGKRYYGDEVSDEEEARQFREIMKEI 214

[Close]

BLAST of Cp4.1LG16g01440.1 vs. NCBI nr
Match: gi|1009174379|ref|XP_015868317.1| (PREDICTED: cytochrome P450 81E8-like isoform X2 [Ziziphus jujuba])

HSP 1 Score: 221.5 bits (563), Expect = 1.2e-54
Identity = 110/174 (63.22%), Postives = 141/174 (81.03%), Query Frame = 1


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP 60
           ++GHL+LLK PVH+T  +LS KYG +F+L LGS+ VVVVSS S V ECFT NDI+LA+RP
Sbjct: 41  IVGHLHLLKHPVHRTYHRLSQKYGPIFSLWLGSRRVVVVSSASAVHECFTKNDIVLASRP 100

Query: 61  SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLC 120
           + +I KHLTYN+T+ + +PYGDHWR LRRI +IEIFS++RLNMFL  RKDE+K LL KL 
Sbjct: 101 ALHIRKHLTYNSTVMTVAPYGDHWRNLRRIGSIEIFSSSRLNMFLGIRKDEVKRLLLKLS 160

Query: 121 CISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQI 175
             S   F RVEM S++SEL+ NI+MRMV GKR+YG+ VSD E+AR+FR+I+K+I
Sbjct: 161 QNSVQDFKRVEMKSLLSELTFNIIMRMVSGKRYYGDEVSDEEEARQFREIMKEI 214

[Close]

BLAST of Cp4.1LG16g01440.1 vs. NCBI nr
Match: gi|1009171671|ref|XP_015866864.1| (PREDICTED: cytochrome P450 81E8-like [Ziziphus jujuba])

HSP 1 Score: 217.6 bits (553), Expect = 1.8e-53
Identity = 108/173 (62.43%), Postives = 137/173 (79.19%), Query Frame = 1


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP 60
           ++GHL+LLK PVH+T ++LS+KYG +++LR GS+ VV+VSSPS V+ECFT NDI+LANRP
Sbjct: 45  IIGHLHLLKLPVHRTFRRLSHKYGPIYSLRFGSRRVVIVSSPSAVEECFTKNDIVLANRP 104

Query: 61  SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLC 120
              + KH+ YNNT   ASPYGDHWR LRRI  IEIFS++RLNMFL  RKDEIK LL KL 
Sbjct: 105 QLLVGKHIGYNNTTIVASPYGDHWRNLRRIGAIEIFSSSRLNMFLGVRKDEIKRLLLKLS 164

Query: 121 CISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQ 174
             S  SF +VE+ SM SEL+ NIVMRM  GKR+YG+GV+  E+AR+FR I+++
Sbjct: 165 RNSVHSFAKVELKSMFSELTFNIVMRMGAGKRYYGDGVTHEEEARQFRAIIRE 217

[Close]

BLAST of Cp4.1LG16g01440.1 vs. NCBI nr
Match: gi|645220075|ref|XP_008238612.1| (PREDICTED: cytochrome P450 81D11 [Prunus mume])

HSP 1 Score: 213.8 bits (543), Expect = 2.5e-52
Identity = 106/175 (60.57%), Postives = 134/175 (76.57%), Query Frame = 1


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP 60
           +LGHL+LLK PVH+T  +LS K+G +F+L  GSQ VV+VSSPS V+ECFT NDI+LANRP
Sbjct: 45  ILGHLHLLKAPVHRTFHRLSQKHGPIFSLWFGSQRVVIVSSPSAVQECFTRNDIVLANRP 104

Query: 61  SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLC 120
           S  + KH+ YN+T  S SPYGDHWR LRRI  IEIFS+ RLN F  TRKDE+KHL+ KL 
Sbjct: 105 SLLLFKHIGYNSTTISTSPYGDHWRNLRRIGAIEIFSSARLNTFANTRKDEVKHLICKLA 164

Query: 121 CISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVS-DLEQARKFRDIVKQI 175
             S   F +VE+ SM +EL+ NI+M MV GKR+YG+ VS D E+A++FR I+K+I
Sbjct: 165 QNSVHEFAKVELKSMFTELTFNIIMTMVAGKRYYGDDVSVDKEEAKQFRQIMKEI 219

The following BLAST results are available for this feature:

BLAST of Cp4.1LG16g01440.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
C81E8_MEDTR	5.5e-47	54.24	Cytochrome P450 81E8 OS=Medicago truncatula GN=CYP81E8 PE=2 SV=1	[more]
C81E1_GLYEC	1.2e-41	47.46	Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2	[more]
C81E9_MEDTR	2.6e-41	46.99	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula GN=CYP81E9 PE=1 SV=1	[more]
C81D1_ARATH	1.0e-40	49.44	Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CYP81D1 PE=2 SV=1	[more]
C8D11_ARATH	3.2e-39	46.63	Cytochrome P450 81D11 OS=Arabidopsis thaliana GN=CYP81D11 PE=2 SV=1	[more]

BLAST of Cp4.1LG16g01440.1 vs. TrEMBL


Match Name	E-value	Identity	Description
M5WME3_PRUPE	3.0e-52	60.00	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa025296mg PE=3 SV=1	[more]
M5WQ76_PRUPE	5.1e-52	59.43	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa026692mg PE=3 SV=1	[more]
M5WJL2_PRUPE	6.7e-52	60.00	Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa017987mg PE=3 S...	[more]
W9R990_9ROSA	1.1e-51	61.93	Cytochrome P450 81D1 OS=Morus notabilis GN=L484_009303 PE=3 SV=1	[more]
A0A0A0LTD0_CUCSA	4.4e-51	58.89	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G425740 PE=3 SV=1	[more]

BLAST of Cp4.1LG16g01440.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G23190.1	2.9e-46	51.70	cytochrome P450, family 81, subfamily D, polypeptide 7	[more]
AT2G23220.1	1.2e-44	50.85	cytochrome P450, family 81, subfamily D, polypeptide 6	[more]
AT4G37370.1	1.2e-44	49.15	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]
AT4G37320.1	6.6e-43	46.89	cytochrome P450, family 81, subfamily D, polypeptide 5	[more]
AT4G37330.1	2.5e-42	49.71	cytochrome P450, family 81, subfamily D, polypeptide 4	[more]

BLAST of Cp4.1LG16g01440.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|1009174377\|ref\|XP_015868316.1\|	1.2e-54	63.22	PREDICTED: cytochrome P450 81E8-like isoform X1 [Ziziphus jujuba]	[more]
gi\|1009174371\|ref\|XP_015868313.1\|	1.2e-54	63.22	PREDICTED: cytochrome P450 81E8-like [Ziziphus jujuba]	[more]
gi\|1009174379\|ref\|XP_015868317.1\|	1.2e-54	63.22	PREDICTED: cytochrome P450 81E8-like isoform X2 [Ziziphus jujuba]	[more]
gi\|1009171671\|ref\|XP_015866864.1\|	1.8e-53	62.43	PREDICTED: cytochrome P450 81E8-like [Ziziphus jujuba]	[more]
gi\|645220075\|ref\|XP_008238612.1\|	2.5e-52	60.57	PREDICTED: cytochrome P450 81D11 [Prunus mume]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0020037	heme binding
GO:0005506	iron ion binding
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Vocabulary: **INTERPRO**
Term	Definition
IPR002401	Cyt_P450_E_grp-I
IPR001128	Cyt_P450

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cp4.1LG16g01440	Cp4.1LG16g01440	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG16g01440.1:cds:001	Cp4.1LG16g01440.1:cds:001	CDS
Cp4.1LG16g01440.1:cds:002	Cp4.1LG16g01440.1:cds:002	CDS
Cp4.1LG16g01440.1:cds:003	Cp4.1LG16g01440.1:cds:003	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG16g01440.1	Cp4.1LG16g01440.1-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	GENE3D	G3DSA:1.10.630.10		coord: 2..174 score: 6.1
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 2..166 score: 7.7
IPR001128	Cytochrome P450	unknown	SSF48264	Cytochrome P450	coord: 2..177 score: 4.45
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 44..65 score: 1.4E-10coord: 20..39 score: 1.4E-10coord: 139..157 score: 1.4
None	No IPR available	PANTHER	PTHR24298	FAMILY NOT NAMED	coord: 1..179 score: 2.1
None	No IPR available	PANTHER	PTHR24298:SF116	SUBFAMILY NOT NAMED	coord: 1..179 score: 2.1