Cp4.1LG15g03650.1 (mRNA) Cucurbita pepo (Zucchini)

NameCp4.1LG15g03650.1
TypemRNA
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionCystatin domain containing protein
LocationCp4.1LG15 : 4903552 .. 4903923 (-)
Sequence length372
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTTCTACCATGCTCGCTAACATCGGTCAACACGATCCCATCCTCCATGTCACAAAGGATGCAAAGGGCGATCATCGTTCTGAATGGATATCGATTCGCGCTATCGAGGACCCCTACGTTCAAGAAGTTGCTAAGTTCGTGGTGGTCGAGGTGAACAAGGAAAAGAAGGCAACTTTTCAATATAAGGGTTTGCTACGTTGTTGGGTTAAGGAGGAGATGGGAGGTATCAAATATCGCCTTATTTTGGAGGTAAAAGGAGGTGCTTCCGAAGGTAAATATGAGGCTATTGTTTGGGAGGCTTCTGGTCGTCTCATAATAAGAAAGCTGAAGCTTGTATCTTTTGTGGCTTGTAATCATGGAGTTGAATGA

mRNA sequence

ATGATTTCTACCATGCTCGCTAACATCGGTCAACACGATCCCATCCTCCATGTCACAAAGGATGCAAAGGGCGATCATCGTTCTGAATGGATATCGATTCGCGCTATCGAGGACCCCTACGTTCAAGAAGTTGCTAAGTTCGTGGTGGTCGAGGTGAACAAGGAAAAGAAGGCAACTTTTCAATATAAGGGTTTGCTACGTTGTTGGGTTAAGGAGGAGATGGGAGGTATCAAATATCGCCTTATTTTGGAGGTAAAAGGAGGTGCTTCCGAAGGTAAATATGAGGCTATTGTTTGGGAGGCTTCTGGTCGTCTCATAATAAGAAAGCTGAAGCTTGTATCTTTTGTGGCTTGTAATCATGGAGTTGAATGA

Coding sequence (CDS)

ATGATTTCTACCATGCTCGCTAACATCGGTCAACACGATCCCATCCTCCATGTCACAAAGGATGCAAAGGGCGATCATCGTTCTGAATGGATATCGATTCGCGCTATCGAGGACCCCTACGTTCAAGAAGTTGCTAAGTTCGTGGTGGTCGAGGTGAACAAGGAAAAGAAGGCAACTTTTCAATATAAGGGTTTGCTACGTTGTTGGGTTAAGGAGGAGATGGGAGGTATCAAATATCGCCTTATTTTGGAGGTAAAAGGAGGTGCTTCCGAAGGTAAATATGAGGCTATTGTTTGGGAGGCTTCTGGTCGTCTCATAATAAGAAAGCTGAAGCTTGTATCTTTTGTGGCTTGTAATCATGGAGTTGAATGA

Protein sequence

MISTMLANIGQHDPILHVTKDAKGDHRSEWISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKGLLRCWVKEEMGGIKYRLILEVKGGASEGKYEAIVWEASGRLIIRKLKLVSFVACNHGVE
BLAST of Cp4.1LG15g03650.1 vs. Swiss-Prot
Match: CYT1_ACTDE (Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa PE=1 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 2.9e-07
Identity = 27/71 (38.03%), Postives = 42/71 (59.15%), Query Frame = 1

Query: 30  WISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKGLLRCWVKEEMGGIKYRLILEVKGGA 89
           W  I ++    VQ+VA+F V E NK+     QY+ ++R +  + + G  YRL++  K GA
Sbjct: 32  WRPIESLNSAEVQDVAQFAVSEHNKQANDELQYQSVVRGYT-QVVAGTNYRLVIAAKDGA 91

Query: 90  SEGKYEAIVWE 101
             G YEA+VW+
Sbjct: 92  VVGNYEAVVWD 101

BLAST of Cp4.1LG15g03650.1 vs. TrEMBL
Match: A0A0A0K644_CUCSA (Cystatin domain containing protein OS=Cucumis sativus GN=Csa_7G066240 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 3.7e-46
Identity = 96/123 (78.05%), Postives = 105/123 (85.37%), Query Frame = 1

Query: 1   MISTMLANIGQHDPILHVT-KDAKGDHRSEWISIRAIEDPYVQEVAKFVVVEVNKEKKAT 60
           MIST+LAN+GQHDPILHVT KD KGDH+SEWISIRAIEDPYVQEVAKF + E  KEK A 
Sbjct: 1   MISTLLANVGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAMREFEKEKMAR 60

Query: 61  FQYKGLLRCWVKEEMGGIKYRLILEV-KGGASEGKYEAIVWEASGRLIIRKLKLVSFVAC 120
           FQYK LL CW+KEEMGGIKYRL+LEV K    EGKYEA+VWEASGRLIIRKLKL+SF+  
Sbjct: 61  FQYKRLLSCWIKEEMGGIKYRLVLEVIKEEKLEGKYEAVVWEASGRLIIRKLKLISFMPS 120

Query: 121 NHG 122
            HG
Sbjct: 121 MHG 123

BLAST of Cp4.1LG15g03650.1 vs. TrEMBL
Match: A0A0B4ZWL9_MORAL (Cysteine proteinase inhibitor OS=Morus alba var. atropurpurea PE=2 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 3.1e-08
Identity = 30/71 (42.25%), Postives = 47/71 (66.20%), Query Frame = 1

Query: 30  WISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKGLLRCWVKEEMGGIKYRLILEVKGGA 89
           W  I+ + DP+V+E+A F V E NK+ KA+ + K +++    + + G  YRL+L VK GA
Sbjct: 29  WTPIKDLSDPHVKEIANFAVSEYNKQSKASLKLKSIVK-GETQVVAGTNYRLVLAVKNGA 88

Query: 90  SEGKYEAIVWE 101
           +  +YEA+VWE
Sbjct: 89  TAERYEAVVWE 98

BLAST of Cp4.1LG15g03650.1 vs. TrEMBL
Match: W9R7F6_9ROSA (Cysteine proteinase inhibitor OS=Morus notabilis GN=L484_004273 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 3.4e-07
Identity = 28/71 (39.44%), Postives = 46/71 (64.79%), Query Frame = 1

Query: 30  WISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKGLLRCWVKEEMGGIKYRLILEVKGGA 89
           W  I+ + DP+V+E+A F V E NK+ KA+ + K +++    + + G  YRL++ V  GA
Sbjct: 29  WTPIKDLSDPHVKEIANFAVSEYNKQSKASLKLKSIVK-GETQVVAGTNYRLVVAVTNGA 88

Query: 90  SEGKYEAIVWE 101
           +  +YEA+VWE
Sbjct: 89  AAERYEAVVWE 98

BLAST of Cp4.1LG15g03650.1 vs. TrEMBL
Match: A0A0D2VC84_GOSRA (Cysteine proteinase inhibitor OS=Gossypium raimondii GN=B456_010G187500 PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.0e-06
Identity = 28/71 (39.44%), Postives = 46/71 (64.79%), Query Frame = 1

Query: 30  WISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKGLLRCWVKEEMGGIKYRLILEVKGGA 89
           W  I+ I+DP+V E+AKF V E NK+ K + +   +++    + + GI Y+L+L+ K G+
Sbjct: 33  WTPIKDIKDPHVMEIAKFAVDEYNKQSKVSLKLDEVVK-GETQVVSGINYKLVLKAKDGS 92

Query: 90  SEGKYEAIVWE 101
           +   YEA+VWE
Sbjct: 93  AVNTYEAVVWE 102

BLAST of Cp4.1LG15g03650.1 vs. TrEMBL
Match: A0A0A0LTF5_CUCSA (Cysteine proteinase inhibitor OS=Cucumis sativus GN=Csa_1G183580 PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.0e-06
Identity = 31/79 (39.24%), Postives = 46/79 (58.23%), Query Frame = 1

Query: 23  KGDHRSEWISIRAIEDPYVQEVAKFVVVEVNKEKKAT-FQYKGLLRCWVKEEMGGIKYRL 82
           KG     W  I  ++DP+V+E+ KF V E NK+ K    ++K ++     + + G  YRL
Sbjct: 22  KGSMLGGWSKIPDLKDPHVEEIGKFAVAEYNKQSKGVAIEFKSVVS-GETQVVAGTNYRL 81

Query: 83  ILEVKGGASEGKYEAIVWE 101
           +++ K G S  KYEAIVWE
Sbjct: 82  LIDAKRGESMSKYEAIVWE 99

BLAST of Cp4.1LG15g03650.1 vs. NCBI nr
Match: gi|700188534|gb|KGN43767.1| (Cystatin domain containing protein [Cucumis sativus])

HSP 1 Score: 192.2 bits (487), Expect = 5.4e-46
Identity = 96/123 (78.05%), Postives = 105/123 (85.37%), Query Frame = 1

Query: 1   MISTMLANIGQHDPILHVT-KDAKGDHRSEWISIRAIEDPYVQEVAKFVVVEVNKEKKAT 60
           MIST+LAN+GQHDPILHVT KD KGDH+SEWISIRAIEDPYVQEVAKF + E  KEK A 
Sbjct: 1   MISTLLANVGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAMREFEKEKMAR 60

Query: 61  FQYKGLLRCWVKEEMGGIKYRLILEV-KGGASEGKYEAIVWEASGRLIIRKLKLVSFVAC 120
           FQYK LL CW+KEEMGGIKYRL+LEV K    EGKYEA+VWEASGRLIIRKLKL+SF+  
Sbjct: 61  FQYKRLLSCWIKEEMGGIKYRLVLEVIKEEKLEGKYEAVVWEASGRLIIRKLKLISFMPS 120

Query: 121 NHG 122
            HG
Sbjct: 121 MHG 123

BLAST of Cp4.1LG15g03650.1 vs. NCBI nr
Match: gi|746657137|gb|AJD79055.1| (CPI-4 [Morus alba var. atropurpurea])

HSP 1 Score: 66.2 bits (160), Expect = 4.4e-08
Identity = 30/71 (42.25%), Postives = 47/71 (66.20%), Query Frame = 1

Query: 30  WISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKGLLRCWVKEEMGGIKYRLILEVKGGA 89
           W  I+ + DP+V+E+A F V E NK+ KA+ + K +++    + + G  YRL+L VK GA
Sbjct: 29  WTPIKDLSDPHVKEIANFAVSEYNKQSKASLKLKSIVK-GETQVVAGTNYRLVLAVKNGA 88

Query: 90  SEGKYEAIVWE 101
           +  +YEA+VWE
Sbjct: 89  TAERYEAVVWE 98

BLAST of Cp4.1LG15g03650.1 vs. NCBI nr
Match: gi|703092669|ref|XP_010094696.1| (Cysteine proteinase inhibitor 5 [Morus notabilis])

HSP 1 Score: 62.8 bits (151), Expect = 4.9e-07
Identity = 28/71 (39.44%), Postives = 46/71 (64.79%), Query Frame = 1

Query: 30  WISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKGLLRCWVKEEMGGIKYRLILEVKGGA 89
           W  I+ + DP+V+E+A F V E NK+ KA+ + K +++    + + G  YRL++ V  GA
Sbjct: 29  WTPIKDLSDPHVKEIANFAVSEYNKQSKASLKLKSIVK-GETQVVAGTNYRLVVAVTNGA 88

Query: 90  SEGKYEAIVWE 101
           +  +YEA+VWE
Sbjct: 89  AAERYEAVVWE 98

BLAST of Cp4.1LG15g03650.1 vs. NCBI nr
Match: gi|659127356|ref|XP_008463660.1| (PREDICTED: cysteine proteinase inhibitor 5 [Cucumis melo])

HSP 1 Score: 62.4 bits (150), Expect = 6.4e-07
Identity = 29/72 (40.28%), Postives = 46/72 (63.89%), Query Frame = 1

Query: 30  WISIRAIEDPYVQEVAKFVVVEVNKEKKAT-FQYKGLLRCWVKEEMGGIKYRLILEVKGG 89
           W  I  ++DP+V+E+AKF V + N++ K    ++KG+      + + G  YRL+++ K G
Sbjct: 32  WSKISDVKDPHVEEIAKFAVAQYNEQSKGVAIEFKGV-ESGETQVVAGTNYRLVIDAKRG 91

Query: 90  ASEGKYEAIVWE 101
            S GKYEA+VWE
Sbjct: 92  ESIGKYEALVWE 102

BLAST of Cp4.1LG15g03650.1 vs. NCBI nr
Match: gi|823138315|ref|XP_012469025.1| (PREDICTED: cysteine proteinase inhibitor 5-like, partial [Gossypium raimondii])

HSP 1 Score: 61.6 bits (148), Expect = 1.1e-06
Identity = 34/99 (34.34%), Postives = 59/99 (59.60%), Query Frame = 1

Query: 18  VTKDAKGDHRSE-WISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKGLLRCWVKEEMGG 77
           +  DA+ D   E W SI+ I+DP+V E+ +F V E N + K + + + +++    + + G
Sbjct: 5   ILSDARKDALIEGWTSIKNIKDPHVTEITEFAVDEHNTQLKGSLKLEEVVK-GETQVISG 64

Query: 78  IKYRLILEVKGGASEGKYEAIVWEASGRLIIRKLKLVSF 116
           I Y+L+L+ K G ++  YEA+VWE   +  ++  KL SF
Sbjct: 65  INYKLVLQAKDGTADNSYEAVVWE---KAWLKFRKLTSF 99

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CYT1_ACTDE2.9e-0738.03Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K644_CUCSA3.7e-4678.05Cystatin domain containing protein OS=Cucumis sativus GN=Csa_7G066240 PE=4 SV=1[more]
A0A0B4ZWL9_MORAL3.1e-0842.25Cysteine proteinase inhibitor OS=Morus alba var. atropurpurea PE=2 SV=1[more]
W9R7F6_9ROSA3.4e-0739.44Cysteine proteinase inhibitor OS=Morus notabilis GN=L484_004273 PE=3 SV=1[more]
A0A0D2VC84_GOSRA1.0e-0639.44Cysteine proteinase inhibitor OS=Gossypium raimondii GN=B456_010G187500 PE=3 SV=... [more]
A0A0A0LTF5_CUCSA1.0e-0639.24Cysteine proteinase inhibitor OS=Cucumis sativus GN=Csa_1G183580 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
gi|700188534|gb|KGN43767.1|5.4e-4678.05Cystatin domain containing protein [Cucumis sativus][more]
gi|746657137|gb|AJD79055.1|4.4e-0842.25CPI-4 [Morus alba var. atropurpurea][more]
gi|703092669|ref|XP_010094696.1|4.9e-0739.44Cysteine proteinase inhibitor 5 [Morus notabilis][more]
gi|659127356|ref|XP_008463660.1|6.4e-0740.28PREDICTED: cysteine proteinase inhibitor 5 [Cucumis melo][more]
gi|823138315|ref|XP_012469025.1|1.1e-0634.34PREDICTED: cysteine proteinase inhibitor 5-like, partial [Gossypium raimondii][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR020381Proteinase inhibitor I25, cystatin, conserved region
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cp4.1LG15g03650Cp4.1LG15g03650gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG15g03650.1:cds:001Cp4.1LG15g03650.1:cds:001CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cp4.1LG15g03650.1Cp4.1LG15g03650.1-proteinpolypeptide


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020381Proteinase inhibitor I25, cystatin, conserved regionPFAMPF16845SQAPIcoord: 33..102
score: 3.7
NoneNo IPR availableGENE3DG3DSA:3.10.450.10coord: 34..101
score: 1.
NoneNo IPR availableunknownSSF54403Cystatin/monellincoord: 28..115
score: 3.4