Cp4.1LG11g03030.1 (mRNA) Cucurbita pepo (Zucchini)

NameCp4.1LG11g03030.1
TypemRNA
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionMajor facilitator superfamily protein
LocationCp4.1LG11 : 1586785 .. 1589499 (-)
Sequence length1737
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCTCTACGAAGCTCCAAAAATGGCGAAACGACCAAATTCCGATCCCTCCGACAGCAACAAACTCCTCTCAATCATGGCACAGCCATTCCGGTGGCTTAAAATGCTCTCATCGGAGCTAAACCCGAGTTTCATCGCCGGCGTGGTGCTCGTCTACGGCTTAAGCCACGGCTTCTCCGGCTCCTATTTCAAAGTCGTATCCGATTACTATTGGAAAGACGTGCAGAAGCTACAGCCGTCGGTGGTTCAGATCTACATCGGGATTTACACGATTCCATGGGTGATGAAGCCGATTTGGGGGCTATTGACGGACGCGTTTCCGGTGAGAGGGTACCTACGGAGGCCGTATTTCGTGATTGCGGGGGTGGTTGGGACGGCGTCGGCGGTGGCGGTGGCGATGAAGGAAGGATTGGGGATTTTGGGAGCTCTAGGGCTTTTGATTGGGATTTCGGCGGCGATGGCGATTGCGGATGTGACGATCGATGCTTGCATTGTGCGGAGTAGCATTGAAGTGCGGTGGTTGGCGCAGGATTTGCAGAGCCTCTGTGGGGCTTGCTCTTCCATTGGCCACTTGATTGGGTATTCCACCAGTGGCGTTTTTGTTCATCTCCTCGGCGCTCAGGTCTGTAAAAACTAAAAAATAATATGAAAATACATGAAAATAAAAATGTGAAATGACGGTTTTCTCCTTGGTCCTTATGACCTTTTATTTTGCAGTTTAGTCCTTGAATTAGTTACTAATTACTAAGAAGCGAATGTGCAGGAAGCACTTGGGATCCTGGCAATTCCCCCAGCCATGATAATCGTGTTGGGCTTCTTCATACATGAGCCACGCTCCTCCACCCTCCAATCTTATGGGAAGCCCAAGGTCTTTTTCTCCCTAAAATACCCTCAACTTTTTATTTATTTATTTAATATATATATATTAGAGTTATCTTGTTTCTAAATATTCAAAACTATATTTCAAATAAAAATGACCGTGTTTGAAACGACCCAATTAGGGTTTCGATTTTAAGTACGTATGTACACTTTTATAGGCTATATTTCTTCGAAATTTAGGTTGAAATCATGGAATTTGAACATTTCACGCAACAATATTGAAAAGTATCGACTTTTACTCAATTTTTCACGCGCGTGTTTCAAAGGACCAAACTAGGGTTTCTAAATAAAAGTTTCGATTTTAAGTCTGTACCTACATTGTTTTTGCTCTTTTTATTCAAAATTTGAGTCGAAATACCCGAATATGAACATTCCCCACAACAATGTTTTAAAATATCGATGATTTTTTTTATGCGTGAGTTTGGAACGACCTAATTGGGATTTCGATTTTAAGCACGTATGTACACTTTTGTAGGTTATATTTCTTCGAAATTTGAGTTTATTAAAATTTAGAACGAAATTCTATGTCTCATTTTTTATTTAAAAATTATAATGTATTTTTATGTAATTTTGAAATATTTATAAGAATTTAAAATCATGTAAAATAAATTAATTCATAATATATATGCAGGGAGAGTCTTTGAAGCAAGTGGGAGTGGCCATAAGAGACACGTGCAAAGCCATCAAATATCCGCACGTGTGGAAGCCTGCGCTTTTCATGTTCCTCTGTCTCTCTCTCAGTATAAGTACTCACGAGGGCCAATTCTATTGGTACACGGACAAGAAAGCGGGCCCAGCCTTCTCTCAGGTCTTCGACATGTCGTTTATAGCCTTCTACTCCTATTGAGTACAAACGTCATGTCGTTTTCTTCAACAATCTCTTGCTTTATACTCGCAGGAATCGGTGGGGCTCATTTACGCAGTCGGCTCAGCGGCGTCTCTAATCGGAGTACTAATCTACCACAAAACGCTCAGAGACGTAAAATTCCGACGAATCCTCTTCTTCGCTCAGCTTTTCTACGGCGTCGCCGGACTTCTCGATGTAGTTTTCATCCTCCGGTGGAATTTAGCCCTAAAAATTCCGGATGAGTTATTCGTCGTTTTGGAAGAGTGCGTTTCTCGAATCGTGTCGCGAATCCGATGGACGCCGATGATGGTTCTGAACACTCGACTCTGTTCCGTCGGGATCGAAGGAACGTTCTTCGCATTATTGGCTTGTATTGATAGTCTAGGCATGCTGTGCTCGAAATGGAGCGGCGGATTGGTGCTCCATGCTTTAGGAGTCACGAGGAGTGATTTTAGGAACCTTTGGTTGGTGGTTTTGCTTCGGAGTCTGCTGAGATTTGTGGTTCTGGCGTTCGTTTTTCTTGTTCCTGATGCAAGTCAATCGGACATTTTGGTTCCGGCCGATCAGACGGTGAGTAGAAAAAGTTCGACGACTGTGGAGGAAGATGGCGATAGCATTCCTCTCGTCTCCATGAAAGGAGAGGGCCGAGGTTAGTGCGTTGGCGCGTCGGATTTGATAAGTAAGAGGAACGCTGTGCGAGTTCGGGAAGGCATAGAAACGAGGGTATAATTTTGATGAGGAAGAACGTCAATGTTTAGGTAATAAGGTTATAATTTGGTGTTATTATAATTTTCAAATTTTATACTCCTAATATTGTAACTAGATATTATTGCCTCTTTTTTACCTTTAGGATGGACTCAAAGTTTTAAAATGAGTCTATTAGGACAAATTTCCACCCGAATGTTTTATTTTTTGTCTCCAATAGATGTAATTGTTGGGGGTGAAAGTCACACATCGGCTAATTTAGAGAATGATCGTGTGTGTTTA

mRNA sequence

ATGGATCTCTACGAAGCTCCAAAAATGGCGAAACGACCAAATTCCGATCCCTCCGACAGCAACAAACTCCTCTCAATCATGGCACAGCCATTCCGGTGGCTTAAAATGCTCTCATCGGAGCTAAACCCGAGTTTCATCGCCGGCGTGGTGCTCGTCTACGGCTTAAGCCACGGCTTCTCCGGCTCCTATTTCAAAGTCGTATCCGATTACTATTGGAAAGACGTGCAGAAGCTACAGCCGTCGGTGGTTCAGATCTACATCGGGATTTACACGATTCCATGGGTGATGAAGCCGATTTGGGGGCTATTGACGGACGCGTTTCCGGTGAGAGGGTACCTACGGAGGCCGTATTTCGTGATTGCGGGGGTGGTTGGGACGGCGTCGGCGGTGGCGGTGGCGATGAAGGAAGGATTGGGGATTTTGGGAGCTCTAGGGCTTTTGATTGGGATTTCGGCGGCGATGGCGATTGCGGATGTGACGATCGATGCTTGCATTGTGCGGAGTAGCATTGAAGTGCGGTGGTTGGCGCAGGATTTGCAGAGCCTCTGTGGGGCTTGCTCTTCCATTGGCCACTTGATTGGGTATTCCACCAGTGGCGTTTTTGTTCATCTCCTCGGCGCTCAGGGAGAGTCTTTGAAGCAAGTGGGAGTGGCCATAAGAGACACGTGCAAAGCCATCAAATATCCGCACGTGTGGAAGCCTGCGCTTTTCATGTTCCTCTGTCTCTCTCTCAGTATAAGTACTCACGAGGGCCAATTCTATTGGTACACGGACAAGAAAGCGGGCCCAGCCTTCTCTCAGGAATCGGTGGGGCTCATTTACGCAGTCGGCTCAGCGGCGTCTCTAATCGGAGTACTAATCTACCACAAAACGCTCAGAGACGTAAAATTCCGACGAATCCTCTTCTTCGCTCAGCTTTTCTACGGCGTCGCCGGACTTCTCGATGTAGTTTTCATCCTCCGGTGGAATTTAGCCCTAAAAATTCCGGATGAGTTATTCGTCGTTTTGGAAGAGTGCGTTTCTCGAATCGTGTCGCGAATCCGATGGACGCCGATGATGGTTCTGAACACTCGACTCTGTTCCGTCGGGATCGAAGGAACGTTCTTCGCATTATTGGCTTGTATTGATAGTCTAGGCATGCTGTGCTCGAAATGGAGCGGCGGATTGGTGCTCCATGCTTTAGGAGTCACGAGGAGTGATTTTAGGAACCTTTGGTTGGTGGTTTTGCTTCGGAGTCTGCTGAGATTTGTGGTTCTGGCGTTCGTTTTTCTTGTTCCTGATGCAAGTCAATCGGACATTTTGGTTCCGGCCGATCAGACGGTGAGTAGAAAAAGTTCGACGACTGTGGAGGAAGATGGCGATAGCATTCCTCTCGTCTCCATGAAAGGAGAGGGCCGAGGTTAGTGCGTTGGCGCGTCGGATTTGATAAGTAAGAGGAACGCTGTGCGAGTTCGGGAAGGCATAGAAACGAGGGTATAATTTTGATGAGGAAGAACGTCAATGTTTAGGTAATAAGGTTATAATTTGGTGTTATTATAATTTTCAAATTTTATACTCCTAATATTGTAACTAGATATTATTGCCTCTTTTTTACCTTTAGGATGGACTCAAAGTTTTAAAATGAGTCTATTAGGACAAATTTCCACCCGAATGTTTTATTTTTTGTCTCCAATAGATGTAATTGTTGGGGGTGAAAGTCACACATCGGCTAATTTAGAGAATGATCGTGTGTGTTTA

Coding sequence (CDS)

ATGGATCTCTACGAAGCTCCAAAAATGGCGAAACGACCAAATTCCGATCCCTCCGACAGCAACAAACTCCTCTCAATCATGGCACAGCCATTCCGGTGGCTTAAAATGCTCTCATCGGAGCTAAACCCGAGTTTCATCGCCGGCGTGGTGCTCGTCTACGGCTTAAGCCACGGCTTCTCCGGCTCCTATTTCAAAGTCGTATCCGATTACTATTGGAAAGACGTGCAGAAGCTACAGCCGTCGGTGGTTCAGATCTACATCGGGATTTACACGATTCCATGGGTGATGAAGCCGATTTGGGGGCTATTGACGGACGCGTTTCCGGTGAGAGGGTACCTACGGAGGCCGTATTTCGTGATTGCGGGGGTGGTTGGGACGGCGTCGGCGGTGGCGGTGGCGATGAAGGAAGGATTGGGGATTTTGGGAGCTCTAGGGCTTTTGATTGGGATTTCGGCGGCGATGGCGATTGCGGATGTGACGATCGATGCTTGCATTGTGCGGAGTAGCATTGAAGTGCGGTGGTTGGCGCAGGATTTGCAGAGCCTCTGTGGGGCTTGCTCTTCCATTGGCCACTTGATTGGGTATTCCACCAGTGGCGTTTTTGTTCATCTCCTCGGCGCTCAGGGAGAGTCTTTGAAGCAAGTGGGAGTGGCCATAAGAGACACGTGCAAAGCCATCAAATATCCGCACGTGTGGAAGCCTGCGCTTTTCATGTTCCTCTGTCTCTCTCTCAGTATAAGTACTCACGAGGGCCAATTCTATTGGTACACGGACAAGAAAGCGGGCCCAGCCTTCTCTCAGGAATCGGTGGGGCTCATTTACGCAGTCGGCTCAGCGGCGTCTCTAATCGGAGTACTAATCTACCACAAAACGCTCAGAGACGTAAAATTCCGACGAATCCTCTTCTTCGCTCAGCTTTTCTACGGCGTCGCCGGACTTCTCGATGTAGTTTTCATCCTCCGGTGGAATTTAGCCCTAAAAATTCCGGATGAGTTATTCGTCGTTTTGGAAGAGTGCGTTTCTCGAATCGTGTCGCGAATCCGATGGACGCCGATGATGGTTCTGAACACTCGACTCTGTTCCGTCGGGATCGAAGGAACGTTCTTCGCATTATTGGCTTGTATTGATAGTCTAGGCATGCTGTGCTCGAAATGGAGCGGCGGATTGGTGCTCCATGCTTTAGGAGTCACGAGGAGTGATTTTAGGAACCTTTGGTTGGTGGTTTTGCTTCGGAGTCTGCTGAGATTTGTGGTTCTGGCGTTCGTTTTTCTTGTTCCTGATGCAAGTCAATCGGACATTTTGGTTCCGGCCGATCAGACGGTGAGTAGAAAAAGTTCGACGACTGTGGAGGAAGATGGCGATAGCATTCCTCTCGTCTCCATGAAAGGAGAGGGCCGAGGTTAG

Protein sequence

MDLYEAPKMAKRPNSDPSDSNKLLSIMAQPFRWLKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTASAVAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSSIGHLIGYSTSGVFVHLLGAQGESLKQVGVAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLALKIPDELFVVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHALGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPADQTVSRKSSTTVEEDGDSIPLVSMKGEGRG
BLAST of Cp4.1LG11g03030.1 vs. Swiss-Prot
Match: FBT6_ARATH (Probable folate-biopterin transporter 6 OS=Arabidopsis thaliana GN=At5g10820 PE=2 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 1.6e-154
Identity = 278/487 (57.08%), Postives = 350/487 (71.87%), Query Frame = 1

Query: 12  RPNSDPSD------SNKLLSIMAQPFRWLKMLSSELNPSFIAGVVLVYGLSHGFSGSYFK 71
           +P SD S+       N ++S++ QPF+WL+MLSS LN SF+ GVVLVYG++ GFSGS FK
Sbjct: 18  KPISDHSEIKTKARRNAVVSVLIQPFQWLQMLSSRLNLSFVLGVVLVYGVNQGFSGSIFK 77

Query: 72  VVSDYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVG 131
           VV+DYYWKDVQ++QPSVVQ+Y+G+Y IPWVM+PIWGL TD FP++GY R+PYFV++GV+G
Sbjct: 78  VVTDYYWKDVQQVQPSVVQLYMGLYYIPWVMRPIWGLFTDVFPIKGYKRKPYFVVSGVLG 137

Query: 132 TASAVAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGA 191
             SA+A+ +   L    AL  L+G+SAAMAIADV IDACI  +SI +R LA D+QSLC  
Sbjct: 138 LVSAIAIVVLGKLPAALALSCLLGVSAAMAIADVVIDACIATNSINIRSLAPDIQSLCMV 197

Query: 192 CSSIGHLIGYSTSGVFVHLLGAQG---------------------------------ESL 251
           CSS G L+GY+TSGVFVH LG QG                                 +  
Sbjct: 198 CSSAGALVGYATSGVFVHQLGPQGALGVLAFSPATIVILGFFIYEKRSSTVPTQKTKKDT 257

Query: 252 KQVGVAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGL 311
             +GVA++  CK +KYP VWKP+L+MF+ L+L+ISTHEG FYWYTD  AGPAFSQE VG+
Sbjct: 258 DGLGVAVKGMCKTVKYPEVWKPSLYMFISLALNISTHEGHFYWYTDPTAGPAFSQEFVGI 317

Query: 312 IYAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLALKIPDEL 371
           IYAVG+ AS+ GVLIYHK L+   FR ILFFAQL Y  +G+LD+VFI RWNL L IPD L
Sbjct: 318 IYAVGALASMFGVLIYHKKLKGYSFRNILFFAQLLYVFSGMLDLVFIKRWNLTLGIPDSL 377

Query: 372 FVVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLH 431
           FV+ EE  ++++S+IRW PM+VL+TRLC +GIEGTFFA L CIDS G L SKW GG VLH
Sbjct: 378 FVITEESFTKMISKIRWIPMVVLSTRLCPLGIEGTFFAFLMCIDSFGQLASKWGGGFVLH 437

Query: 432 ALGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPADQTVSRKSSTTVEED 460
           A GVTR DF NLWLV+L+R++LR V + FVFLVPD+   D LVP+D    ++S    ++D
Sbjct: 438 AFGVTRHDFGNLWLVILIRNILRLVTVCFVFLVPDSDHLDDLVPSDVLPKKQSE---DDD 497

BLAST of Cp4.1LG11g03030.1 vs. Swiss-Prot
Match: FBT3_ARATH (Probable folate-biopterin transporter 3 OS=Arabidopsis thaliana GN=At1g79710 PE=2 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 3.9e-113
Identity = 212/481 (44.07%), Postives = 307/481 (63.83%), Query Frame = 1

Query: 10  AKRPNSDPSDSNKLLSIMAQPFRWLKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVSD 69
           ++R + D      L  ++ +P RWL+ML  EL+ SF  GV++VYG+S G      KV + 
Sbjct: 14  SRRNHQDKKKEGVLDLVLKRPLRWLRMLIEELHWSFFFGVIIVYGVSQGLGKGLSKVSTQ 73

Query: 70  YYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTASA 129
           YY+KD QK+QPS  QIY+G+  IPW++KP+WGLLTD  PV GY RRPYF++AG +   S 
Sbjct: 74  YYFKDEQKIQPSQAQIYVGLIQIPWIIKPLWGLLTDVVPVLGYRRRPYFILAGFLAMISM 133

Query: 130 VAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSSI 189
           + + +   L +  AL  L+  SA +AIADVTIDAC+ + SI    LA D+QSLCG CSSI
Sbjct: 134 MVLWLHTNLHLALALSCLVAGSAGVAIADVTIDACVTQCSISHPTLAADMQSLCGLCSSI 193

Query: 190 GHLIGYSTSGVFVHLLGAQGES---------LKQVGVAIRDT------------------ 249
           G L+G+S SGV VHL+G++G           L  VG+ ++++                  
Sbjct: 194 GSLVGFSLSGVLVHLVGSKGVYGLLGVTAGLLVVVGMVLKESPSRSLGRKHVNDKFLDAG 253

Query: 250 ---CKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSA 309
               K  +Y  VW+P LFM L  ++S+  HEG FYWYTD K GP+FS+E+VG I + G+ 
Sbjct: 254 SAIWKTFQYGEVWRPCLFMLLSAAVSLHIHEGMFYWYTDSKDGPSFSKEAVGSIMSFGAI 313

Query: 310 ASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLALKIPDELFVVLEEC 369
            SL+G+L+Y   L++  FR ++F+A     ++G LD++ +LR NL L +PD  F+V++E 
Sbjct: 314 GSLVGILLYQNFLKNFPFRNVVFWALSLSVLSGFLDLILVLRINLKLGLPDYFFIVVDEF 373

Query: 370 VSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHALGVTRS 429
           VS ++SRI+W P++VL+++LC  G+EGTFFALL  I+ +G L S W GG++LHAL VTR+
Sbjct: 374 VSHMISRIKWLPLLVLSSKLCPAGMEGTFFALLMSIEHIGHLVSSWGGGVLLHALKVTRT 433

Query: 430 DFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPADQTVSRKSSTTVEEDGDSIPLV 461
            F NLWLV+++RSLLR + +  VFL+P+   +  ++PA+   SR S     E  +   L+
Sbjct: 434 QFDNLWLVIVIRSLLRVIPIGLVFLIPNVDSNSTILPAEMLTSRISEGVDTEKIEMTALI 493

BLAST of Cp4.1LG11g03030.1 vs. Swiss-Prot
Match: FBT2_ARATH (Probable folate-biopterin transporter 2 OS=Arabidopsis thaliana GN=At5g25050 PE=2 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 2.1e-106
Identity = 201/457 (43.98%), Postives = 295/457 (64.55%), Query Frame = 1

Query: 25  SIMAQPFRWLKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQ 84
           +++  P RWLKMLSSEL+ SF+ GVV +YG++ G  GS  +V ++YY KDVQK+QPS  Q
Sbjct: 25  NVVCGPVRWLKMLSSELHWSFVFGVVSLYGINQGLGGSLGRVATEYYMKDVQKVQPSESQ 84

Query: 85  IYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTASAVAVAMKEGLGILGAL 144
               I  IPW++KP+WG+LTD  P+ G+ RRPYF++AGV+G  S + +++   L +  AL
Sbjct: 85  ALTAITKIPWIIKPLWGILTDVLPIFGFHRRPYFILAGVLGVVSLLFISLHSNLHLYLAL 144

Query: 145 GLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSSIGHLIGYSTSGVFVHL 204
             +   SAAMAIADVTIDAC   +SI+   LA D+QSLC   SSIG L+G+  SG+ VHL
Sbjct: 145 FWMTISSAAMAIADVTIDACTAYNSIKHPSLASDMQSLCSLSSSIGALLGFFMSGILVHL 204

Query: 205 LGAQGE--------------------------SLKQVGVAIRDTCKAI----KYPHVWKP 264
           +G++G                           S KQV     D  KA+    K   VW+P
Sbjct: 205 VGSKGVFGLLTFPFALVSVVGIVFSEPHVPGFSYKQVNQKFTDAGKAMWRTMKCSDVWRP 264

Query: 265 ALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKTLRD 324
           +L+M++ L+L ++ HEG FYW+TD K GP F+QE+VG I ++GS  S++   +Y   L+D
Sbjct: 265 SLYMYISLTLGLNIHEGLFYWFTDSKDGPLFAQETVGFILSIGSIGSILAATLYQLVLKD 324

Query: 325 VKFRRILFFAQLFYGVAGLLDVVFILRWNLALKIPDELFVVLEECVSRIVSRIRWTPMMV 384
             FR +  + QL + ++G+LD++ + R NL   +PD LF+V++E VS+++ R++W P++V
Sbjct: 325 HPFRGLCLWTQLLFALSGMLDLILVFRLNLKFGLPDYLFIVVDEIVSQMIGRLKWMPLLV 384

Query: 385 LNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHALGVTRSDFRNLWLVVLLRSLL 444
           L ++LC  GIEGTFFALL  ID+ G++ S W GG++LH L VTR++F NLWL VL+R+++
Sbjct: 385 LTSKLCPHGIEGTFFALLMSIDNAGLMTSSWLGGIMLHVLKVTRTEFGNLWLAVLVRNVM 444

Query: 445 RFVVLAFVFLVPDASQSDILVPADQTVSRKSSTTVEE 452
           R + L F+FLVP   Q+   +P D+ +   S    +E
Sbjct: 445 RLLPLCFLFLVPKGDQNTFKLP-DEIMGEDSDEEKDE 480

BLAST of Cp4.1LG11g03030.1 vs. Swiss-Prot
Match: FBT5_ARATH (Probable folate-biopterin transporter 5 OS=Arabidopsis thaliana GN=At5g25040 PE=2 SV=2)

HSP 1 Score: 360.1 bits (923), Expect = 3.5e-98
Identity = 192/446 (43.05%), Postives = 286/446 (64.13%), Query Frame = 1

Query: 21  NKLLSIMAQPFRWLKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVSDYYWKDVQKLQP 80
           + L S++  P RWLKML+S+L+ S++  VV   G++ GF  S   V +DYY KDVQK+QP
Sbjct: 21  SNLRSVVCGPVRWLKMLASKLHWSYVFAVVSNCGINQGFGYSLGHVATDYYMKDVQKVQP 80

Query: 81  SVVQIYIGIYTIPWVM-KPIWGLLTDAFPVRGYLRRPYFVIAGVVGTASAVAVAMKEGLG 140
           S  Q    I  I W++ KP++G+LTD  P+ G+ RRPYF++AGV+G  S + ++++  L 
Sbjct: 81  SQYQALSAITRISWIIFKPLFGILTDVLPIFGFHRRPYFILAGVIGVVSLLFISLQSNLH 140

Query: 141 ILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSSIGHLIGYSTSG 200
           +  AL  +   SAAMAIADVTIDAC   +S +   LA D+QSLC    SIG L+G+  SG
Sbjct: 141 LYLALFWMTISSAAMAIADVTIDACTTYNSNKHPSLASDMQSLCSLSYSIGELLGFFMSG 200

Query: 201 VFVHLLGAQG---------ESLKQVGV------------------AIRDTCKAIKYPHVW 260
           + VHL+G++G           +  VGV                  A++   + IK   VW
Sbjct: 201 ILVHLVGSKGVFGLLTFTFALVSVVGVLFSEPRVHGFSFKQNFTNAMKAMWRTIKCSDVW 260

Query: 261 KPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKTL 320
           +P+L+MF+  +L +   EG FYW+TD K GP F+QE+VG I ++GS  S++GVL+Y+  L
Sbjct: 261 QPSLYMFITRALGLDIKEGLFYWFTDSKDGPLFAQETVGFILSIGSIGSILGVLLYNLRL 320

Query: 321 RDVKFRRILFFAQLFYGVAGLLDVVFILRWNLALKIPDELFVVLEECVSRIVSRIRWTPM 380
           +D  FR++  + QL + ++G+ D++ +LR NL   +PD LF+V++  VS+++ R+ W  +
Sbjct: 321 KDHPFRKLFLWTQLLFALSGMFDLILVLRLNLKFGLPDYLFIVVDGIVSKMIIRLTWMVI 380

Query: 381 MVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHALGVTRSDFRNLWLVVLLRS 439
            VLNT+LC  GIEGTFFALL  ID+ G++ S W GG +LH L VTR++F NLWL VL+R+
Sbjct: 381 FVLNTKLCPHGIEGTFFALLMSIDNAGLMTSSWLGGKMLHVLKVTRTEFGNLWLAVLVRN 440

BLAST of Cp4.1LG11g03030.1 vs. Swiss-Prot
Match: FBT_SYNY3 (Folate-biopterin transporter OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=slr0642 PE=1 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 3.6e-34
Identity = 115/390 (29.49%), Postives = 194/390 (49.74%), Query Frame = 1

Query: 65  KVVSDYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVV 124
           ++   ++ KD   L P+ +   IG+   PW++KP+ GL++D  P+ GY RR Y  ++G++
Sbjct: 53  RLAVSFFLKDELGLSPAAMGALIGLGAAPWILKPVLGLMSDTVPLFGYRRRSYLWLSGLM 112

Query: 125 GTASAVAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIV-RSSIEVRWLAQDLQSLC 184
           G+A  +  A     G    L LL   S ++AI DV +D+ +V R+  E       LQSL 
Sbjct: 113 GSAGWLLFAAWVSSGTQAGLVLLF-TSLSVAIGDVIVDSLVVERAQRESLAQVGSLQSLT 172

Query: 185 GACSSIGHLI----------GYSTSGVFV-----------------HLLGAQGESLKQVG 244
              +++G +I           +ST  VF                   +  A+ E   Q  
Sbjct: 173 WGAAAVGGIITAYASGALLEWFSTRTVFAITAIFPLLTVGAAFLISEVSTAEEEEKPQPK 232

Query: 245 VAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAV 304
             I+   +A++   +  P LF+F     +  + E  F+++T  + G  F  + +G +  V
Sbjct: 233 AQIKLVWQAVRQKTILLPTLFIFFW--QATPSAESAFFYFTTNELG--FEPKFLGRVRLV 292

Query: 305 GSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLALKIPDELFVVL 364
            S A LIGV +Y + L+ + FR I+ ++ +   + GL  ++ I   N A+ I D  F + 
Sbjct: 293 TSVAGLIGVGLYQRFLKTLPFRVIMGWSTVISSLLGLTTLILITHANRAMGIDDHWFSLG 352

Query: 365 EECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHALGV 424
           +  +  +  +I + P++VL  RLC  GIE T FALL  + +L  + S   G L+ H LGV
Sbjct: 353 DSIILTVTGQIAFMPVLVLAARLCPPGIEATLFALLMSVMNLAGVLSFEVGSLLTHWLGV 412

Query: 425 TRSDFRNLWLVVLLRSLLRFVVLAFVFLVP 427
           T + F NL L+V++ +L   + L F+ L+P
Sbjct: 413 TETQFDNLALLVIITNLSTLLPLPFLGLLP 437

BLAST of Cp4.1LG11g03030.1 vs. TrEMBL
Match: A0A0A0LMJ9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G382710 PE=4 SV=1)

HSP 1 Score: 770.8 bits (1989), Expect = 9.7e-220
Identity = 393/484 (81.20%), Postives = 428/484 (88.43%), Query Frame = 1

Query: 18  SDSNKLLSIMAQPFRWLKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVSDYYWKDVQK 77
           +++NKL SIM QPFRWLKMLS+EL+PSFIAGV+L+YGLSHGFSGSYFKVVSDYYWKDVQK
Sbjct: 64  TNNNKLFSIMLQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQK 123

Query: 78  LQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTASAVAVAMKEG 137
           LQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVI+GVVG  +AV +A+K G
Sbjct: 124 LQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGG 183

Query: 138 LGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSSIGHLIGYST 197
           LG+L ALGLLIGIS AMAIADVTIDACIVR+SIEV+WLAQDLQSLCGACSSIGHLIGYST
Sbjct: 184 LGVLEALGLLIGISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYST 243

Query: 198 SGVFVHLLG-----------------------------------AQGESLKQVGVAIRDT 257
           SG FVHLLG                                   ++G S+KQVGVAIRDT
Sbjct: 244 SGFFVHLLGAQEALGILAIPPALVVLLGFFIHETPSPTLQYNGKSKGGSIKQVGVAIRDT 303

Query: 258 CKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASL 317
           CKAIKYPHVW+P+LFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASL
Sbjct: 304 CKAIKYPHVWRPSLFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASL 363

Query: 318 IGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLALKIPDELFVVLEECVSR 377
           IGVLIYHKTLRD+KFRRILFFAQLFYGVAG LDV+F+LRWNLAL+IPDELFVV+EECV+R
Sbjct: 364 IGVLIYHKTLRDLKFRRILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTR 423

Query: 378 IVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHALGVTRSDFR 437
           IV+RIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLG LCSKWSGGLVLHA GVTRSDFR
Sbjct: 424 IVTRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFR 483

Query: 438 NLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPADQTVSRKSSTTVEEDGDSIPLVSMK 467
           NLWLVVLLR++LRFVV+ FVFLVPDA+Q+DIL+P D T+ RKSS+   EDGD+IPLVSMK
Sbjct: 484 NLWLVVLLRTVLRFVVVGFVFLVPDANQTDILIPGDHTMVRKSSS---EDGDNIPLVSMK 543

BLAST of Cp4.1LG11g03030.1 vs. TrEMBL
Match: W9RP11_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_025493 PE=4 SV=1)

HSP 1 Score: 605.1 bits (1559), Expect = 7.0e-170
Identity = 314/506 (62.06%), Postives = 389/506 (76.88%), Query Frame = 1

Query: 1   MDLYEAPKMAKRPNSDPSD---------SNKLL--SIMAQPFRWLKMLSSELNPSFIAGV 60
           M+  E+  +   P+ DPSD         SN LL   I  +PFRWL+MLSS+L+P+F+AGV
Sbjct: 1   MNSTESRNLVGEPDGDPSDEPNKTDDHHSNHLLITRIFLEPFRWLQMLSSQLSPTFVAGV 60

Query: 61  VLVYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPV 120
           VLVYGL+ GFSGS+FKVVSDYYWKDVQK+QPS VQ+Y G+YTIPWVMKPIWGLLTD FPV
Sbjct: 61  VLVYGLNQGFSGSFFKVVSDYYWKDVQKVQPSAVQLYSGLYTIPWVMKPIWGLLTDVFPV 120

Query: 121 RGYLRRPYFVIAGVVGTASAVAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSS 180
           RG+ RRPYFV+AGVVG  SAVAVA+  G+    A+G L+G+SAA+AIADVTIDACI R+S
Sbjct: 121 RGFRRRPYFVLAGVVGALSAVAVALDGGMSAAAAVGCLMGLSAAVAIADVTIDACIARNS 180

Query: 181 IEVRWLAQDLQSLCGACSSIGHLIGYSTSGVFVHLL---GAQG----------------- 240
           IE+R LA DLQSLCG CSS+G L+GYS+SG FVH L   GA G                 
Sbjct: 181 IEIRALASDLQSLCGFCSSVGALLGYSSSGFFVHHLGPQGALGLLAVPPALLVVLGFFIY 240

Query: 241 -----------ESLKQVGVAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTD 300
                      ++++++GVA++   K IK+P VWKP+L+M+L L+LSISTHEGQFYWYTD
Sbjct: 241 EQRTISSHSEKKAVEKLGVAMKGMYKTIKFPQVWKPSLYMYLSLALSISTHEGQFYWYTD 300

Query: 301 KKAGPAFSQESVGLIYAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVF 360
            KAGPAFSQE VG+IYA+G+ AS++GVLIYHKTL+D  FR ILF AQL YGV+G++D++F
Sbjct: 301 PKAGPAFSQEFVGIIYAIGAMASIVGVLIYHKTLKDHPFRNILFLAQLLYGVSGMIDLIF 360

Query: 361 ILRWNLALKIPDELFVVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSL 420
           ILRWNLAL IPD  FVV+EECV RIVSRIRW PM+VL++RLC +GIEGTFFALL CIDSL
Sbjct: 361 ILRWNLALGIPDYFFVVMEECVHRIVSRIRWMPMIVLSSRLCPLGIEGTFFALLMCIDSL 420

Query: 421 GMLCSKWSGGLVLHALGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPAD 465
           G L SKW GG+VLH L VTR+DF NLWLVVL+R++LRF  LAF+FLVP A QSD+L+P+D
Sbjct: 421 GGLSSKWGGGMVLHLLHVTRTDFTNLWLVVLIRNVLRFSTLAFIFLVPKAGQSDVLIPSD 480

BLAST of Cp4.1LG11g03030.1 vs. TrEMBL
Match: A0A061GIF0_THECC (Major facilitator superfamily protein OS=Theobroma cacao GN=TCM_036893 PE=4 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 1.4e-165
Identity = 297/475 (62.53%), Postives = 365/475 (76.84%), Query Frame = 1

Query: 18  SDSNKLLSIMAQPFRWLKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVSDYYWKDVQK 77
           S+ + + S+  +PF+WL+MLSS+LNP+FI G+VLVYGLS GFS S+FKVV+DYYWKDVQK
Sbjct: 25  SNQSNIFSLFLEPFQWLQMLSSQLNPTFIFGIVLVYGLSQGFSSSFFKVVTDYYWKDVQK 84

Query: 78  LQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTASAVAVAMKEG 137
           +QPSVVQ+YIG+Y IPW+MKP+WGL TD FP++GY RRPYFV+AGVVG ASA+ VA+   
Sbjct: 85  VQPSVVQLYIGLYYIPWIMKPVWGLFTDVFPIKGYRRRPYFVLAGVVGGASALMVALFGN 144

Query: 138 LGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSSIGHLIGYST 197
           L    ALG LIG++A MAIADVTIDACI R+SI+VR LA D+QSLCG CSS G LIGYST
Sbjct: 145 LPAALALGCLIGVAAGMAIADVTIDACIARNSIKVRSLAPDMQSLCGFCSSAGALIGYST 204

Query: 198 SGVFVHLLGAQG--------------------------------ESLKQVGVAIRDTCKA 257
           SG FVH LGAQG                                ++++ +GVA+R   + 
Sbjct: 205 SGFFVHHLGAQGALILLAIPPVFITVLGFVIYEMRSTSLQSEKQKAMETLGVALRGMYQT 264

Query: 258 IKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGV 317
           IK+P VWKP+L+M+L L+LSISTHEGQFYWYTD KAGPAFSQE VG+IYA+G+ AS++GV
Sbjct: 265 IKFPQVWKPSLYMYLSLALSISTHEGQFYWYTDPKAGPAFSQEFVGVIYAIGAMASMVGV 324

Query: 318 LIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLALKIPDELFVVLEECVSRIVS 377
           LIYHKTL+D+ FR +LFFAQL YG +G+LD++FILRWNL L IPD  FV++EECVSRI+S
Sbjct: 325 LIYHKTLKDIPFRNLLFFAQLLYGTSGMLDLIFILRWNLVLGIPDYFFVIMEECVSRIIS 384

Query: 378 RIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHALGVTRSDFRNLW 437
           RIRW PM+VL+TRLC +GIEGTFFALL CIDSLG L SKW GG+VLH L VTR+DF NLW
Sbjct: 385 RIRWIPMIVLSTRLCPLGIEGTFFALLMCIDSLGSLTSKWGGGIVLHTLHVTRTDFTNLW 444

Query: 438 LVVLLRSLLRFVVLAFVFLVPDASQSDILVPADQTVSRKSSTTVEEDGDSIPLVS 461
           LVVL+R+ LRF  L  +FLVP A QSD L+P D      +++TV  D + + LVS
Sbjct: 445 LVVLIRNTLRFATLGLIFLVPKADQSDALIPPDILT---NNSTVTADDEGLELVS 496

BLAST of Cp4.1LG11g03030.1 vs. TrEMBL
Match: A0A0D2UH96_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_009G063700 PE=4 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 4.6e-161
Identity = 285/476 (59.87%), Postives = 358/476 (75.21%), Query Frame = 1

Query: 14  NSDPSDSNKLLSIMAQPFRWLKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVSDYYWK 73
           +++ S S+ + S+  +P +W++MLSS+LNP+FI G+V+VYGL+ GFSGS+FKVV+DYYWK
Sbjct: 32  SAEASKSSNIFSVFLEPIQWIQMLSSQLNPTFIIGIVIVYGLNLGFSGSFFKVVTDYYWK 91

Query: 74  DVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTASAVAVA 133
           DVQK+QPSVVQ+YIG+Y IPW+MKP+WGLLTD FP+RGY RRPYFV+AGV+G  SA+ VA
Sbjct: 92  DVQKVQPSVVQLYIGLYYIPWIMKPVWGLLTDVFPIRGYRRRPYFVLAGVLGGVSALMVA 151

Query: 134 MKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSSIGHLI 193
           +   L    AL   IGI+A MAIADVTIDACI  +SIEVR LA D+QSLCG CSS G LI
Sbjct: 152 LIGNLPAALALSCFIGIAAGMAIADVTIDACIATNSIEVRSLAPDMQSLCGFCSSAGALI 211

Query: 194 GYSTSGVFVHLLGAQG--------------------------------ESLKQVGVAIRD 253
           GYSTSG FVH LGAQG                                + +  +GVA++ 
Sbjct: 212 GYSTSGFFVHHLGAQGALILMSIPPVFITVLGFVIYEMRSPCLHPEKQKKMGTLGVAVKG 271

Query: 254 TCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAAS 313
             + IK+P VWKP+L+M+L L+LSISTHEGQFYWYTD KAGP FSQESVG IYA+G+ AS
Sbjct: 272 MYQTIKFPQVWKPSLYMYLSLALSISTHEGQFYWYTDAKAGPGFSQESVGAIYAIGAMAS 331

Query: 314 LIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLALKIPDELFVVLEECVS 373
           ++GVLIYHKTL+D+ FR +LFFAQL YG +G+LD++FILRWNL L IPD  FV+ EEC+S
Sbjct: 332 MVGVLIYHKTLKDIPFRNLLFFAQLLYGASGMLDLIFILRWNLVLGIPDYFFVITEECIS 391

Query: 374 RIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHALGVTRSDF 433
           RI+SRIRW PM+VL+TRLC +GIEGTFFALL CIDSLG L SKW GG+VLH   VTR+DF
Sbjct: 392 RIISRIRWIPMIVLSTRLCPLGIEGTFFALLMCIDSLGSLTSKWGGGMVLHVFHVTRTDF 451

Query: 434 RNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPADQTVSRKSSTTVEEDGDSIP 458
            NLWLV+L+R++LR   L  +FLVP A  SD L+P D  ++  + T+ +E  + +P
Sbjct: 452 TNLWLVILIRNILRIATLGLIFLVPKADPSDALIPQDILMTHSTVTSDDEGLELVP 507

BLAST of Cp4.1LG11g03030.1 vs. TrEMBL
Match: M5XFR9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005422mg PE=4 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 1.7e-160
Identity = 291/459 (63.40%), Postives = 353/459 (76.91%), Query Frame = 1

Query: 36  MLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIYIGIYTIPWV 95
           MLSSEL+P+F+ GVV+VYGLS GFSGSYF+VV+D+YWKDV+K+QPSVVQ+YIG+Y IPWV
Sbjct: 1   MLSSELDPTFVLGVVIVYGLSQGFSGSYFRVVADFYWKDVRKVQPSVVQLYIGLYYIPWV 60

Query: 96  MKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTASAVAVAMKEGLGILGALGLLIGISAAMA 155
           MKPIWG+LTD FPV+GY RRPYFV+AG+ G  SA+ VA+ + L    AL  LIG+ A++A
Sbjct: 61  MKPIWGILTDVFPVQGYRRRPYFVLAGLTGIISALMVALSDSLAAAVALTCLIGVMASVA 120

Query: 156 IADVTIDACIVRSSIEVRWLAQDLQSLCGACSSIGHLIGYSTSGVFVHLLGAQG------ 215
           IADVTIDACI R+SIE+RWLA DLQSLCG CSS G L+GYSTSG FVH LGAQG      
Sbjct: 121 IADVTIDACIARNSIEIRWLASDLQSLCGFCSSAGALVGYSTSGFFVHHLGAQGALGLLA 180

Query: 216 --------------------------ESLKQVGVAIRDTCKAIKYPHVWKPALFMFLCLS 275
                                     + +++VGVAIR   + IK P VWKP+L+M+L L+
Sbjct: 181 IPPVFLTVLGFVIYEEGSTSLRSVKKKVVEEVGVAIRGMYRTIKCPQVWKPSLYMYLSLT 240

Query: 276 LSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKTLRDVKFRRILFF 335
           LSISTHEGQFYWYTD  AGPAFSQE VG+IYAVG+ AS++GVLIYH TL+D  FR +LFF
Sbjct: 241 LSISTHEGQFYWYTDPTAGPAFSQEFVGMIYAVGAMASIVGVLIYHNTLKDYPFRNLLFF 300

Query: 336 AQLFYGVAGLLDVVFILRWNLALKIPDELFVVLEECVSRIVSRIRWTPMMVLNTRLCSVG 395
           AQL Y ++G+LD+ FILRWNL L IPD  FV++EECVSRIVSRIRW PM+VL+TRLC +G
Sbjct: 301 AQLLYAISGMLDLTFILRWNLILGIPDYFFVIMEECVSRIVSRIRWMPMIVLSTRLCPLG 360

Query: 396 IEGTFFALLACIDSLGMLCSKWSGGLVLHALGVTRSDFRNLWLVVLLRSLLRFVVLAFVF 455
           IEGTFFALL CIDSLG L SKW GG+VLHAL VTR+DF NLWLV+L+R++LRF  LA +F
Sbjct: 361 IEGTFFALLMCIDSLGSLSSKWGGGVVLHALNVTRTDFTNLWLVILIRNVLRFSTLALIF 420

Query: 456 LVPDASQSDILVPADQTVSRKSSTTVEEDGDSIPLVSMK 463
           LVP A Q D+L+P+D  +  K+S     D +S+ LV MK
Sbjct: 421 LVPKADQFDVLIPSD--LLTKTSAVSAVDDESLELVPMK 457

BLAST of Cp4.1LG11g03030.1 vs. TAIR10
Match: AT5G10820.1 (AT5G10820.1 Major facilitator superfamily protein)

HSP 1 Score: 547.4 bits (1409), Expect = 8.8e-156
Identity = 278/487 (57.08%), Postives = 350/487 (71.87%), Query Frame = 1

Query: 12  RPNSDPSD------SNKLLSIMAQPFRWLKMLSSELNPSFIAGVVLVYGLSHGFSGSYFK 71
           +P SD S+       N ++S++ QPF+WL+MLSS LN SF+ GVVLVYG++ GFSGS FK
Sbjct: 18  KPISDHSEIKTKARRNAVVSVLIQPFQWLQMLSSRLNLSFVLGVVLVYGVNQGFSGSIFK 77

Query: 72  VVSDYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVG 131
           VV+DYYWKDVQ++QPSVVQ+Y+G+Y IPWVM+PIWGL TD FP++GY R+PYFV++GV+G
Sbjct: 78  VVTDYYWKDVQQVQPSVVQLYMGLYYIPWVMRPIWGLFTDVFPIKGYKRKPYFVVSGVLG 137

Query: 132 TASAVAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGA 191
             SA+A+ +   L    AL  L+G+SAAMAIADV IDACI  +SI +R LA D+QSLC  
Sbjct: 138 LVSAIAIVVLGKLPAALALSCLLGVSAAMAIADVVIDACIATNSINIRSLAPDIQSLCMV 197

Query: 192 CSSIGHLIGYSTSGVFVHLLGAQG---------------------------------ESL 251
           CSS G L+GY+TSGVFVH LG QG                                 +  
Sbjct: 198 CSSAGALVGYATSGVFVHQLGPQGALGVLAFSPATIVILGFFIYEKRSSTVPTQKTKKDT 257

Query: 252 KQVGVAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGL 311
             +GVA++  CK +KYP VWKP+L+MF+ L+L+ISTHEG FYWYTD  AGPAFSQE VG+
Sbjct: 258 DGLGVAVKGMCKTVKYPEVWKPSLYMFISLALNISTHEGHFYWYTDPTAGPAFSQEFVGI 317

Query: 312 IYAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLALKIPDEL 371
           IYAVG+ AS+ GVLIYHK L+   FR ILFFAQL Y  +G+LD+VFI RWNL L IPD L
Sbjct: 318 IYAVGALASMFGVLIYHKKLKGYSFRNILFFAQLLYVFSGMLDLVFIKRWNLTLGIPDSL 377

Query: 372 FVVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLH 431
           FV+ EE  ++++S+IRW PM+VL+TRLC +GIEGTFFA L CIDS G L SKW GG VLH
Sbjct: 378 FVITEESFTKMISKIRWIPMVVLSTRLCPLGIEGTFFAFLMCIDSFGQLASKWGGGFVLH 437

Query: 432 ALGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPADQTVSRKSSTTVEED 460
           A GVTR DF NLWLV+L+R++LR V + FVFLVPD+   D LVP+D    ++S    ++D
Sbjct: 438 AFGVTRHDFGNLWLVILIRNILRLVTVCFVFLVPDSDHLDDLVPSDVLPKKQSE---DDD 497

BLAST of Cp4.1LG11g03030.1 vs. TAIR10
Match: AT1G79710.1 (AT1G79710.1 Major facilitator superfamily protein)

HSP 1 Score: 409.8 bits (1052), Expect = 2.2e-114
Identity = 212/481 (44.07%), Postives = 307/481 (63.83%), Query Frame = 1

Query: 10  AKRPNSDPSDSNKLLSIMAQPFRWLKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVSD 69
           ++R + D      L  ++ +P RWL+ML  EL+ SF  GV++VYG+S G      KV + 
Sbjct: 14  SRRNHQDKKKEGVLDLVLKRPLRWLRMLIEELHWSFFFGVIIVYGVSQGLGKGLSKVSTQ 73

Query: 70  YYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTASA 129
           YY+KD QK+QPS  QIY+G+  IPW++KP+WGLLTD  PV GY RRPYF++AG +   S 
Sbjct: 74  YYFKDEQKIQPSQAQIYVGLIQIPWIIKPLWGLLTDVVPVLGYRRRPYFILAGFLAMISM 133

Query: 130 VAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSSI 189
           + + +   L +  AL  L+  SA +AIADVTIDAC+ + SI    LA D+QSLCG CSSI
Sbjct: 134 MVLWLHTNLHLALALSCLVAGSAGVAIADVTIDACVTQCSISHPTLAADMQSLCGLCSSI 193

Query: 190 GHLIGYSTSGVFVHLLGAQGES---------LKQVGVAIRDT------------------ 249
           G L+G+S SGV VHL+G++G           L  VG+ ++++                  
Sbjct: 194 GSLVGFSLSGVLVHLVGSKGVYGLLGVTAGLLVVVGMVLKESPSRSLGRKHVNDKFLDAG 253

Query: 250 ---CKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSA 309
               K  +Y  VW+P LFM L  ++S+  HEG FYWYTD K GP+FS+E+VG I + G+ 
Sbjct: 254 SAIWKTFQYGEVWRPCLFMLLSAAVSLHIHEGMFYWYTDSKDGPSFSKEAVGSIMSFGAI 313

Query: 310 ASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLALKIPDELFVVLEEC 369
            SL+G+L+Y   L++  FR ++F+A     ++G LD++ +LR NL L +PD  F+V++E 
Sbjct: 314 GSLVGILLYQNFLKNFPFRNVVFWALSLSVLSGFLDLILVLRINLKLGLPDYFFIVVDEF 373

Query: 370 VSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHALGVTRS 429
           VS ++SRI+W P++VL+++LC  G+EGTFFALL  I+ +G L S W GG++LHAL VTR+
Sbjct: 374 VSHMISRIKWLPLLVLSSKLCPAGMEGTFFALLMSIEHIGHLVSSWGGGVLLHALKVTRT 433

Query: 430 DFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPADQTVSRKSSTTVEEDGDSIPLV 461
            F NLWLV+++RSLLR + +  VFL+P+   +  ++PA+   SR S     E  +   L+
Sbjct: 434 QFDNLWLVIVIRSLLRVIPIGLVFLIPNVDSNSTILPAEMLTSRISEGVDTEKIEMTALI 493

BLAST of Cp4.1LG11g03030.1 vs. TAIR10
Match: AT5G25050.1 (AT5G25050.1 Major facilitator superfamily protein)

HSP 1 Score: 387.5 bits (994), Expect = 1.2e-107
Identity = 201/457 (43.98%), Postives = 295/457 (64.55%), Query Frame = 1

Query: 25  SIMAQPFRWLKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQ 84
           +++  P RWLKMLSSEL+ SF+ GVV +YG++ G  GS  +V ++YY KDVQK+QPS  Q
Sbjct: 25  NVVCGPVRWLKMLSSELHWSFVFGVVSLYGINQGLGGSLGRVATEYYMKDVQKVQPSESQ 84

Query: 85  IYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTASAVAVAMKEGLGILGAL 144
               I  IPW++KP+WG+LTD  P+ G+ RRPYF++AGV+G  S + +++   L +  AL
Sbjct: 85  ALTAITKIPWIIKPLWGILTDVLPIFGFHRRPYFILAGVLGVVSLLFISLHSNLHLYLAL 144

Query: 145 GLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSSIGHLIGYSTSGVFVHL 204
             +   SAAMAIADVTIDAC   +SI+   LA D+QSLC   SSIG L+G+  SG+ VHL
Sbjct: 145 FWMTISSAAMAIADVTIDACTAYNSIKHPSLASDMQSLCSLSSSIGALLGFFMSGILVHL 204

Query: 205 LGAQGE--------------------------SLKQVGVAIRDTCKAI----KYPHVWKP 264
           +G++G                           S KQV     D  KA+    K   VW+P
Sbjct: 205 VGSKGVFGLLTFPFALVSVVGIVFSEPHVPGFSYKQVNQKFTDAGKAMWRTMKCSDVWRP 264

Query: 265 ALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKTLRD 324
           +L+M++ L+L ++ HEG FYW+TD K GP F+QE+VG I ++GS  S++   +Y   L+D
Sbjct: 265 SLYMYISLTLGLNIHEGLFYWFTDSKDGPLFAQETVGFILSIGSIGSILAATLYQLVLKD 324

Query: 325 VKFRRILFFAQLFYGVAGLLDVVFILRWNLALKIPDELFVVLEECVSRIVSRIRWTPMMV 384
             FR +  + QL + ++G+LD++ + R NL   +PD LF+V++E VS+++ R++W P++V
Sbjct: 325 HPFRGLCLWTQLLFALSGMLDLILVFRLNLKFGLPDYLFIVVDEIVSQMIGRLKWMPLLV 384

Query: 385 LNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHALGVTRSDFRNLWLVVLLRSLL 444
           L ++LC  GIEGTFFALL  ID+ G++ S W GG++LH L VTR++F NLWL VL+R+++
Sbjct: 385 LTSKLCPHGIEGTFFALLMSIDNAGLMTSSWLGGIMLHVLKVTRTEFGNLWLAVLVRNVM 444

Query: 445 RFVVLAFVFLVPDASQSDILVPADQTVSRKSSTTVEE 452
           R + L F+FLVP   Q+   +P D+ +   S    +E
Sbjct: 445 RLLPLCFLFLVPKGDQNTFKLP-DEIMGEDSDEEKDE 480

BLAST of Cp4.1LG11g03030.1 vs. TAIR10
Match: AT5G25040.2 (AT5G25040.2 Major facilitator superfamily protein)

HSP 1 Score: 346.3 bits (887), Expect = 3.0e-95
Identity = 192/471 (40.76%), Postives = 286/471 (60.72%), Query Frame = 1

Query: 21  NKLLSIMAQPFRWLKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVSDYYWKDVQKLQP 80
           + L S++  P RWLKML+S+L+ S++  VV   G++ GF  S   V +DYY KDVQK+QP
Sbjct: 21  SNLRSVVCGPVRWLKMLASKLHWSYVFAVVSNCGINQGFGYSLGHVATDYYMKDVQKVQP 80

Query: 81  SVVQIYIGIYTIPWVM-KPIWGLLTDAFPVRGYLRRPYFVIAGVVGTASAVAVAMKEGLG 140
           S  Q    I  I W++ KP++G+LTD  P+ G+ RRPYF++AGV+G  S + ++++  L 
Sbjct: 81  SQYQALSAITRISWIIFKPLFGILTDVLPIFGFHRRPYFILAGVIGVVSLLFISLQSNLH 140

Query: 141 ILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSSIGHLIGYSTSG 200
           +  AL  +   SAAMAIADVTIDAC   +S +   LA D+QSLC    SIG L+G+  SG
Sbjct: 141 LYLALFWMTISSAAMAIADVTIDACTTYNSNKHPSLASDMQSLCSLSYSIGELLGFFMSG 200

Query: 201 VFVHLLGAQG---------ESLKQVGV------------------AIRDTCKAIKYPHVW 260
           + VHL+G++G           +  VGV                  A++   + IK   VW
Sbjct: 201 ILVHLVGSKGVFGLLTFTFALVSVVGVLFSEPRVHGFSFKQNFTNAMKAMWRTIKCSDVW 260

Query: 261 KPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQ------------------------- 320
           +P+L+MF+  +L +   EG FYW+TD K GP F+Q                         
Sbjct: 261 QPSLYMFITRALGLDIKEGLFYWFTDSKDGPLFAQVIPKQVASIFSKSTNMLKAFLVCFQ 320

Query: 321 ESVGLIYAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLALK 380
           E+VG I ++GS  S++GVL+Y+  L+D  FR++  + QL + ++G+ D++ +LR NL   
Sbjct: 321 ETVGFILSIGSIGSILGVLLYNLRLKDHPFRKLFLWTQLLFALSGMFDLILVLRLNLKFG 380

Query: 381 IPDELFVVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSG 439
           +PD LF+V++  VS+++ R+ W  + VLNT+LC  GIEGTFFALL  ID+ G++ S W G
Sbjct: 381 LPDYLFIVVDGIVSKMIIRLTWMVIFVLNTKLCPHGIEGTFFALLMSIDNAGLMTSSWLG 440

BLAST of Cp4.1LG11g03030.1 vs. TAIR10
Match: AT2G32040.1 (AT2G32040.1 Major facilitator superfamily protein)

HSP 1 Score: 100.5 bits (249), Expect = 2.8e-21
Identity = 72/222 (32.43%), Postives = 116/222 (52.25%), Query Frame = 1

Query: 225 AIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIG 284
           AIK P+V+ P LF+FL  +   S  +   +++T  K G  F+ E +G +  V S ASL+G
Sbjct: 344 AIKQPNVFLPTLFIFLWQATPHS--DSAMFYFTTNKLG--FTPEFLGRVKLVTSIASLLG 403

Query: 285 VLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLALKIPDELFVVLEECVSRIV 344
           V +Y+  L+ V  R+I     +F    G+  V+ +  +N  L I DE F + +  +  ++
Sbjct: 404 VGLYNGFLKTVPLRKIFLVTTIFGTGLGMTQVILVSGFNRQLGISDEWFAIGDSLILTVL 463

Query: 345 SRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHALGVTRSDFRNL 404
           ++  + P++VL  RLC  G+E T FA L  I + G +     G  +  A G+TR  F NL
Sbjct: 464 AQASFMPVLVLAARLCPEGMEATLFATLMSISNGGSVLGGLMGAGLTQAFGITRDSFGNL 523

Query: 405 WLVVLLRSLLRFVVLAFVFLVPDASQSDILVPADQTVSRKSS 447
             +++L +L   + L  + L+P  S  D L   D  V  KS+
Sbjct: 524 STLIILCNLSSLLPLPLLGLLPRDS-PDTLTKDDADVEMKSN 560

BLAST of Cp4.1LG11g03030.1 vs. NCBI nr
Match: gi|700207879|gb|KGN62998.1| (hypothetical protein Csa_2G382710 [Cucumis sativus])

HSP 1 Score: 770.8 bits (1989), Expect = 1.4e-219
Identity = 393/484 (81.20%), Postives = 428/484 (88.43%), Query Frame = 1

Query: 18  SDSNKLLSIMAQPFRWLKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVSDYYWKDVQK 77
           +++NKL SIM QPFRWLKMLS+EL+PSFIAGV+L+YGLSHGFSGSYFKVVSDYYWKDVQK
Sbjct: 64  TNNNKLFSIMLQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQK 123

Query: 78  LQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTASAVAVAMKEG 137
           LQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVI+GVVG  +AV +A+K G
Sbjct: 124 LQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGG 183

Query: 138 LGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSSIGHLIGYST 197
           LG+L ALGLLIGIS AMAIADVTIDACIVR+SIEV+WLAQDLQSLCGACSSIGHLIGYST
Sbjct: 184 LGVLEALGLLIGISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYST 243

Query: 198 SGVFVHLLG-----------------------------------AQGESLKQVGVAIRDT 257
           SG FVHLLG                                   ++G S+KQVGVAIRDT
Sbjct: 244 SGFFVHLLGAQEALGILAIPPALVVLLGFFIHETPSPTLQYNGKSKGGSIKQVGVAIRDT 303

Query: 258 CKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASL 317
           CKAIKYPHVW+P+LFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASL
Sbjct: 304 CKAIKYPHVWRPSLFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASL 363

Query: 318 IGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLALKIPDELFVVLEECVSR 377
           IGVLIYHKTLRD+KFRRILFFAQLFYGVAG LDV+F+LRWNLAL+IPDELFVV+EECV+R
Sbjct: 364 IGVLIYHKTLRDLKFRRILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTR 423

Query: 378 IVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHALGVTRSDFR 437
           IV+RIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLG LCSKWSGGLVLHA GVTRSDFR
Sbjct: 424 IVTRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFR 483

Query: 438 NLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPADQTVSRKSSTTVEEDGDSIPLVSMK 467
           NLWLVVLLR++LRFVV+ FVFLVPDA+Q+DIL+P D T+ RKSS+   EDGD+IPLVSMK
Sbjct: 484 NLWLVVLLRTVLRFVVVGFVFLVPDANQTDILIPGDHTMVRKSSS---EDGDNIPLVSMK 543

BLAST of Cp4.1LG11g03030.1 vs. NCBI nr
Match: gi|659088992|ref|XP_008445270.1| (PREDICTED: probable folate-biopterin transporter 6 [Cucumis melo])

HSP 1 Score: 770.4 bits (1988), Expect = 1.8e-219
Identity = 395/499 (79.16%), Postives = 434/499 (86.97%), Query Frame = 1

Query: 9   MAKRPNSDPS-----DSNKLLSIMAQPFRWLKMLSSELNPSFIAGVVLVYGLSHGFSGSY 68
           MA  PNS+ S     +++KL SIM QPFRWLKMLS+EL+PSFIAGV+L+YGLSHGFSGSY
Sbjct: 1   MANEPNSNLSTTTTTNNSKLFSIMLQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSY 60

Query: 69  FKVVSDYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGV 128
           FKVVSDYYWKDVQKLQPSVVQIY GIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVI+GV
Sbjct: 61  FKVVSDYYWKDVQKLQPSVVQIYTGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGV 120

Query: 129 VGTASAVAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLC 188
           VG  +AV VA+K GLG+L ALGLLIG+S AMAIADVTIDACIVR+SIEV+WLAQDLQSLC
Sbjct: 121 VGATAAVGVAVKGGLGVLEALGLLIGVSGAMAIADVTIDACIVRNSIEVQWLAQDLQSLC 180

Query: 189 GACSSIGHLIGYSTSGVFVHLLG-----------------------------------AQ 248
           GACSSIGHLIGYS+SG+FVHLLG                                   ++
Sbjct: 181 GACSSIGHLIGYSSSGIFVHLLGAQEALGILAIPPALIILLGFFIHETPSPTLQYNGKSK 240

Query: 249 GESLKQVGVAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQE 308
           G  + QVGVAIR+TCKA+KYPHVW+P+LFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQE
Sbjct: 241 GGCINQVGVAIRETCKAVKYPHVWRPSLFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQE 300

Query: 309 SVGLIYAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLALKI 368
           SVGLIYAVGSAASLIGVLIYHKTLRD+KFRRILFFAQLFYGVAG LDV+F+LRWNLALKI
Sbjct: 301 SVGLIYAVGSAASLIGVLIYHKTLRDLKFRRILFFAQLFYGVAGFLDVIFVLRWNLALKI 360

Query: 369 PDELFVVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGG 428
           PDELFVV+EECV+RIV+RIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLG LCSKWSGG
Sbjct: 361 PDELFVVIEECVTRIVTRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGTLCSKWSGG 420

Query: 429 LVLHALGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPADQTVSRKS-ST 467
           LVLHA GVTRSDFRNLWLVVLLR++LRFVV+AFVFLVPDA+QSDIL+P D T+ RKS S 
Sbjct: 421 LVLHAFGVTRSDFRNLWLVVLLRTVLRFVVVAFVFLVPDANQSDILIPGDPTMVRKSNSR 480

BLAST of Cp4.1LG11g03030.1 vs. NCBI nr
Match: gi|778672667|ref|XP_011649848.1| (PREDICTED: probable folate-biopterin transporter 6 [Cucumis sativus])

HSP 1 Score: 761.1 bits (1964), Expect = 1.1e-216
Identity = 388/475 (81.68%), Postives = 420/475 (88.42%), Query Frame = 1

Query: 27  MAQPFRWLKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIY 86
           M QPFRWLKMLS+EL+PSFIAGV+L+YGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIY
Sbjct: 1   MLQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIY 60

Query: 87  IGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTASAVAVAMKEGLGILGALGL 146
           IGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVI+GVVG  +AV +A+K GLG+L ALGL
Sbjct: 61  IGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGL 120

Query: 147 LIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSSIGHLIGYSTSGVFVHLLG 206
           LIGIS AMAIADVTIDACIVR+SIEV+WLAQDLQSLCGACSSIGHLIGYSTSG FVHLLG
Sbjct: 121 LIGISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSTSGFFVHLLG 180

Query: 207 -----------------------------------AQGESLKQVGVAIRDTCKAIKYPHV 266
                                              ++G S+KQVGVAIRDTCKAIKYPHV
Sbjct: 181 AQEALGILAIPPALVVLLGFFIHETPSPTLQYNGKSKGGSIKQVGVAIRDTCKAIKYPHV 240

Query: 267 WKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKT 326
           W+P+LFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKT
Sbjct: 241 WRPSLFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKT 300

Query: 327 LRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLALKIPDELFVVLEECVSRIVSRIRWTP 386
           LRD+KFRRILFFAQLFYGVAG LDV+F+LRWNLAL+IPDELFVV+EECV+RIV+RIRWTP
Sbjct: 301 LRDLKFRRILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTRIVTRIRWTP 360

Query: 387 MMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHALGVTRSDFRNLWLVVLLR 446
           MMVLNTRLCSVGIEGTFFALLACIDSLG LCSKWSGGLVLHA GVTRSDFRNLWLVVLLR
Sbjct: 361 MMVLNTRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLR 420

Query: 447 SLLRFVVLAFVFLVPDASQSDILVPADQTVSRKSSTTVEEDGDSIPLVSMKGEGR 467
           ++LRFVV+ FVFLVPDA+Q+DIL+P D T+ RKSS+   EDGD+IPLVSMK EGR
Sbjct: 421 TVLRFVVVGFVFLVPDANQTDILIPGDHTMVRKSSS---EDGDNIPLVSMKSEGR 472

BLAST of Cp4.1LG11g03030.1 vs. NCBI nr
Match: gi|703130080|ref|XP_010104519.1| (hypothetical protein L484_025493 [Morus notabilis])

HSP 1 Score: 605.1 bits (1559), Expect = 1.0e-169
Identity = 314/506 (62.06%), Postives = 389/506 (76.88%), Query Frame = 1

Query: 1   MDLYEAPKMAKRPNSDPSD---------SNKLL--SIMAQPFRWLKMLSSELNPSFIAGV 60
           M+  E+  +   P+ DPSD         SN LL   I  +PFRWL+MLSS+L+P+F+AGV
Sbjct: 1   MNSTESRNLVGEPDGDPSDEPNKTDDHHSNHLLITRIFLEPFRWLQMLSSQLSPTFVAGV 60

Query: 61  VLVYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPV 120
           VLVYGL+ GFSGS+FKVVSDYYWKDVQK+QPS VQ+Y G+YTIPWVMKPIWGLLTD FPV
Sbjct: 61  VLVYGLNQGFSGSFFKVVSDYYWKDVQKVQPSAVQLYSGLYTIPWVMKPIWGLLTDVFPV 120

Query: 121 RGYLRRPYFVIAGVVGTASAVAVAMKEGLGILGALGLLIGISAAMAIADVTIDACIVRSS 180
           RG+ RRPYFV+AGVVG  SAVAVA+  G+    A+G L+G+SAA+AIADVTIDACI R+S
Sbjct: 121 RGFRRRPYFVLAGVVGALSAVAVALDGGMSAAAAVGCLMGLSAAVAIADVTIDACIARNS 180

Query: 181 IEVRWLAQDLQSLCGACSSIGHLIGYSTSGVFVHLL---GAQG----------------- 240
           IE+R LA DLQSLCG CSS+G L+GYS+SG FVH L   GA G                 
Sbjct: 181 IEIRALASDLQSLCGFCSSVGALLGYSSSGFFVHHLGPQGALGLLAVPPALLVVLGFFIY 240

Query: 241 -----------ESLKQVGVAIRDTCKAIKYPHVWKPALFMFLCLSLSISTHEGQFYWYTD 300
                      ++++++GVA++   K IK+P VWKP+L+M+L L+LSISTHEGQFYWYTD
Sbjct: 241 EQRTISSHSEKKAVEKLGVAMKGMYKTIKFPQVWKPSLYMYLSLALSISTHEGQFYWYTD 300

Query: 301 KKAGPAFSQESVGLIYAVGSAASLIGVLIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVF 360
            KAGPAFSQE VG+IYA+G+ AS++GVLIYHKTL+D  FR ILF AQL YGV+G++D++F
Sbjct: 301 PKAGPAFSQEFVGIIYAIGAMASIVGVLIYHKTLKDHPFRNILFLAQLLYGVSGMIDLIF 360

Query: 361 ILRWNLALKIPDELFVVLEECVSRIVSRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSL 420
           ILRWNLAL IPD  FVV+EECV RIVSRIRW PM+VL++RLC +GIEGTFFALL CIDSL
Sbjct: 361 ILRWNLALGIPDYFFVVMEECVHRIVSRIRWMPMIVLSSRLCPLGIEGTFFALLMCIDSL 420

Query: 421 GMLCSKWSGGLVLHALGVTRSDFRNLWLVVLLRSLLRFVVLAFVFLVPDASQSDILVPAD 465
           G L SKW GG+VLH L VTR+DF NLWLVVL+R++LRF  LAF+FLVP A QSD+L+P+D
Sbjct: 421 GGLSSKWGGGMVLHLLHVTRTDFTNLWLVVLIRNVLRFSTLAFIFLVPKAGQSDVLIPSD 480

BLAST of Cp4.1LG11g03030.1 vs. NCBI nr
Match: gi|590571814|ref|XP_007011697.1| (Major facilitator superfamily protein [Theobroma cacao])

HSP 1 Score: 590.9 bits (1522), Expect = 2.0e-165
Identity = 297/475 (62.53%), Postives = 365/475 (76.84%), Query Frame = 1

Query: 18  SDSNKLLSIMAQPFRWLKMLSSELNPSFIAGVVLVYGLSHGFSGSYFKVVSDYYWKDVQK 77
           S+ + + S+  +PF+WL+MLSS+LNP+FI G+VLVYGLS GFS S+FKVV+DYYWKDVQK
Sbjct: 25  SNQSNIFSLFLEPFQWLQMLSSQLNPTFIFGIVLVYGLSQGFSSSFFKVVTDYYWKDVQK 84

Query: 78  LQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVIAGVVGTASAVAVAMKEG 137
           +QPSVVQ+YIG+Y IPW+MKP+WGL TD FP++GY RRPYFV+AGVVG ASA+ VA+   
Sbjct: 85  VQPSVVQLYIGLYYIPWIMKPVWGLFTDVFPIKGYRRRPYFVLAGVVGGASALMVALFGN 144

Query: 138 LGILGALGLLIGISAAMAIADVTIDACIVRSSIEVRWLAQDLQSLCGACSSIGHLIGYST 197
           L    ALG LIG++A MAIADVTIDACI R+SI+VR LA D+QSLCG CSS G LIGYST
Sbjct: 145 LPAALALGCLIGVAAGMAIADVTIDACIARNSIKVRSLAPDMQSLCGFCSSAGALIGYST 204

Query: 198 SGVFVHLLGAQG--------------------------------ESLKQVGVAIRDTCKA 257
           SG FVH LGAQG                                ++++ +GVA+R   + 
Sbjct: 205 SGFFVHHLGAQGALILLAIPPVFITVLGFVIYEMRSTSLQSEKQKAMETLGVALRGMYQT 264

Query: 258 IKYPHVWKPALFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGV 317
           IK+P VWKP+L+M+L L+LSISTHEGQFYWYTD KAGPAFSQE VG+IYA+G+ AS++GV
Sbjct: 265 IKFPQVWKPSLYMYLSLALSISTHEGQFYWYTDPKAGPAFSQEFVGVIYAIGAMASMVGV 324

Query: 318 LIYHKTLRDVKFRRILFFAQLFYGVAGLLDVVFILRWNLALKIPDELFVVLEECVSRIVS 377
           LIYHKTL+D+ FR +LFFAQL YG +G+LD++FILRWNL L IPD  FV++EECVSRI+S
Sbjct: 325 LIYHKTLKDIPFRNLLFFAQLLYGTSGMLDLIFILRWNLVLGIPDYFFVIMEECVSRIIS 384

Query: 378 RIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGMLCSKWSGGLVLHALGVTRSDFRNLW 437
           RIRW PM+VL+TRLC +GIEGTFFALL CIDSLG L SKW GG+VLH L VTR+DF NLW
Sbjct: 385 RIRWIPMIVLSTRLCPLGIEGTFFALLMCIDSLGSLTSKWGGGIVLHTLHVTRTDFTNLW 444

Query: 438 LVVLLRSLLRFVVLAFVFLVPDASQSDILVPADQTVSRKSSTTVEEDGDSIPLVS 461
           LVVL+R+ LRF  L  +FLVP A QSD L+P D      +++TV  D + + LVS
Sbjct: 445 LVVLIRNTLRFATLGLIFLVPKADQSDALIPPDILT---NNSTVTADDEGLELVS 496

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
FBT6_ARATH1.6e-15457.08Probable folate-biopterin transporter 6 OS=Arabidopsis thaliana GN=At5g10820 PE=... [more]
FBT3_ARATH3.9e-11344.07Probable folate-biopterin transporter 3 OS=Arabidopsis thaliana GN=At1g79710 PE=... [more]
FBT2_ARATH2.1e-10643.98Probable folate-biopterin transporter 2 OS=Arabidopsis thaliana GN=At5g25050 PE=... [more]
FBT5_ARATH3.5e-9843.05Probable folate-biopterin transporter 5 OS=Arabidopsis thaliana GN=At5g25040 PE=... [more]
FBT_SYNY33.6e-3429.49Folate-biopterin transporter OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=... [more]
Match NameE-valueIdentityDescription
A0A0A0LMJ9_CUCSA9.7e-22081.20Uncharacterized protein OS=Cucumis sativus GN=Csa_2G382710 PE=4 SV=1[more]
W9RP11_9ROSA7.0e-17062.06Uncharacterized protein OS=Morus notabilis GN=L484_025493 PE=4 SV=1[more]
A0A061GIF0_THECC1.4e-16562.53Major facilitator superfamily protein OS=Theobroma cacao GN=TCM_036893 PE=4 SV=1[more]
A0A0D2UH96_GOSRA4.6e-16159.87Uncharacterized protein OS=Gossypium raimondii GN=B456_009G063700 PE=4 SV=1[more]
M5XFR9_PRUPE1.7e-16063.40Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005422mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G10820.18.8e-15657.08 Major facilitator superfamily protein[more]
AT1G79710.12.2e-11444.07 Major facilitator superfamily protein[more]
AT5G25050.11.2e-10743.98 Major facilitator superfamily protein[more]
AT5G25040.23.0e-9540.76 Major facilitator superfamily protein[more]
AT2G32040.12.8e-2132.43 Major facilitator superfamily protein[more]
Match NameE-valueIdentityDescription
gi|700207879|gb|KGN62998.1|1.4e-21981.20hypothetical protein Csa_2G382710 [Cucumis sativus][more]
gi|659088992|ref|XP_008445270.1|1.8e-21979.16PREDICTED: probable folate-biopterin transporter 6 [Cucumis melo][more]
gi|778672667|ref|XP_011649848.1|1.1e-21681.68PREDICTED: probable folate-biopterin transporter 6 [Cucumis sativus][more]
gi|703130080|ref|XP_010104519.1|1.0e-16962.06hypothetical protein L484_025493 [Morus notabilis][more]
gi|590571814|ref|XP_007011697.1|2.0e-16562.53Major facilitator superfamily protein [Theobroma cacao][more]
The following terms have been associated with this mRNA:
Vocabulary: Cellular Component
TermDefinition
GO:0016021integral component of membrane
Vocabulary: Biological Process
TermDefinition
GO:0006810transport
Vocabulary: INTERPRO
TermDefinition
IPR020846MFS_dom
IPR004324FBT
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006810 transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cp4.1LG11g03030Cp4.1LG11g03030gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG11g03030.1:cds:003Cp4.1LG11g03030.1:cds:003CDS
Cp4.1LG11g03030.1:cds:002Cp4.1LG11g03030.1:cds:002CDS
Cp4.1LG11g03030.1:cds:001Cp4.1LG11g03030.1:cds:001CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cp4.1LG11g03030.1:three_prime_utr:001Cp4.1LG11g03030.1:three_prime_utr:001three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cp4.1LG11g03030.1Cp4.1LG11g03030.1-proteinpolypeptide


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004324Folate-biopterin transporterPFAMPF03092BT1coord: 231..439
score: 1.7E-44coord: 65..208
score: 5.2
IPR020846Major facilitator superfamily domainunknownSSF103473MFS general substrate transportercoord: 49..423
score: 1.44
NoneNo IPR availableGENE3DG3DSA:1.20.1250.20coord: 237..423
score: 5.5E-6coord: 50..208
score: 1.
NoneNo IPR availablePANTHERPTHR31585FAMILY NOT NAMEDcoord: 12..464
score: 4.3E
NoneNo IPR availablePANTHERPTHR31585:SF3FOLATE-BIOPTERIN TRANSPORTER 6-RELATEDcoord: 12..464
score: 4.3E