Cp4.1LG01g03680.1 (mRNA) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG01g03680.1
Type	mRNA
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	21 kDa protein, putative
Location	Cp4.1LG01 : 1646236 .. 1647202 (-)
Sequence length	606

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAATTTCAAATCCTAAAATCGTTACTAACCCTTTTCTTCTTCGTTTCAATTACGTTCACCACCTCGGTCGTGGCTTCTTCTTCATCCGTTACCCGTCCCGTGAAACCATACATCAAAAAGGCTTGCAAAACGACGCCGTATCCACGCCTTTGCGAGATCTCCCTCACCTTACATGCTTCACAAACCAAAAGAACACAACAAGAGTTCTGCAGAGCAACCATGGCTTCTTCTCTCAAAACTACACGAGATGCCACCTCCATAATCTCCAAGTTATCTCGCCAGAAGATGTCTACCTATGAAGCTAAGGTCATTAGAGATTGTGTTGATAATCTTCAAGACTCTACCGACGAGCTCCAAAGAGCCATCGCTGCAATAAAAAACTTGAGTCGTGCTAAAGATGTGAACTTTCAGGTTATTTAAACTTCTACTTAAACCCAAAAGGTTTAAATTTATATGTTTTCGTAAATTTTATCTCAAATTCATATTTTAAACGTCATTCTACATCATGTATTTGAAATAAAATTTTAAACCTAAAATCATCCAAAATATATATATATATATCTTTAGACATGATCTTAGCACACTTATGGTTTGACCATAAGGTTTTAAATACGAAAGTCAACCTAGAGACGTGAAAAATATATATTTAATCAAATAAGTTAACATTTTTTATAAATGAAATTAGGTTGATTATGAATTTTAATTAAACCCTTTAAATAAGTGAAGGTAGATTGGGTTAAATTTAATATTTGTATTCATATTGTGAACACAGTTACATAGTATCAAAACATGGATGAGTGCTGCAGAGACCGATGCTATGACTTGCAGCGATGGGTTGAGCGGCGGCGCTGGTCGGAAGGTGAGGTCGAAGGTGAAGCAAGAGGTGAAGAAGAGTGTTGCGGTTGTGGTGAGGCATATCAGTAACGCCTTGTCCCTCCTCAACACCTTCAAATACAAATCCTGA

mRNA sequence

ATGGAATTTCAAATCCTAAAATCGTTACTAACCCTTTTCTTCTTCGTTTCAATTACGTTCACCACCTCGGTCGTGGCTTCTTCTTCATCCGTTACCCGTCCCGTGAAACCATACATCAAAAAGGCTTGCAAAACGACGCCGTATCCACGCCTTTGCGAGATCTCCCTCACCTTACATGCTTCACAAACCAAAAGAACACAACAAGAGTTCTGCAGAGCAACCATGGCTTCTTCTCTCAAAACTACACGAGATGCCACCTCCATAATCTCCAAGTTATCTCGCCAGAAGATGTCTACCTATGAAGCTAAGGTCATTAGAGATTGTGTTGATAATCTTCAAGACTCTACCGACGAGCTCCAAAGAGCCATCGCTGCAATAAAAAACTTGAGTCGTGCTAAAGATGTGAACTTTCAGTTACATAGTATCAAAACATGGATGAGTGCTGCAGAGACCGATGCTATGACTTGCAGCGATGGGTTGAGCGGCGGCGCTGGTCGGAAGGTGAGGTCGAAGGTGAAGCAAGAGGTGAAGAAGAGTGTTGCGGTTGTGGTGAGGCATATCAGTAACGCCTTGTCCCTCCTCAACACCTTCAAATACAAATCCTGA

Coding sequence (CDS)

ATGGAATTTCAAATCCTAAAATCGTTACTAACCCTTTTCTTCTTCGTTTCAATTACGTTCACCACCTCGGTCGTGGCTTCTTCTTCATCCGTTACCCGTCCCGTGAAACCATACATCAAAAAGGCTTGCAAAACGACGCCGTATCCACGCCTTTGCGAGATCTCCCTCACCTTACATGCTTCACAAACCAAAAGAACACAACAAGAGTTCTGCAGAGCAACCATGGCTTCTTCTCTCAAAACTACACGAGATGCCACCTCCATAATCTCCAAGTTATCTCGCCAGAAGATGTCTACCTATGAAGCTAAGGTCATTAGAGATTGTGTTGATAATCTTCAAGACTCTACCGACGAGCTCCAAAGAGCCATCGCTGCAATAAAAAACTTGAGTCGTGCTAAAGATGTGAACTTTCAGTTACATAGTATCAAAACATGGATGAGTGCTGCAGAGACCGATGCTATGACTTGCAGCGATGGGTTGAGCGGCGGCGCTGGTCGGAAGGTGAGGTCGAAGGTGAAGCAAGAGGTGAAGAAGAGTGTTGCGGTTGTGGTGAGGCATATCAGTAACGCCTTGTCCCTCCTCAACACCTTCAAATACAAATCCTGA

Protein sequence

MEFQILKSLLTLFFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHASQTKRTQQEFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLQDSTDELQRAIAAIKNLSRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSVAVVVRHISNALSLLNTFKYKS

Homology

[Close]

BLAST of Cp4.1LG01g03680.1 vs. Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.4e-22
Identity = 64/187 (34.22%), Postives = 106/187 (56.68%), Query Frame = 1


Query: 17  SITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHASQTKRTQQEFCRATMA 76
           ++ F+  +V  +++   P   +IK +C  T YP +CE SL+ +A   +   QE     + 
Sbjct: 7   TLVFSALLVILAAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTALQ 66

Query: 77  SSLKTTRDATSIISKLSRQK-MSTYEAKVIRDCVDNLQDSTDELQRAIAAIKNLSRAK-- 136
            SL  T+ A + + +L++ K +   +   I DC++ ++DS D + R+   +KNLS AK  
Sbjct: 67  VSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKGN 126

Query: 137 DVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSVAVVVRHISNALSL 196
           D  F++ +++TW+SAA TD  TC DG    AG+ +  K+K+ V+  V  V R  SNAL+L
Sbjct: 127 DFTFRMSNVETWVSAALTDETTCMDGF---AGKGMDGKIKESVRAQVVAVARVTSNALAL 186

Query: 197 LNTFKYK 201
           +N F  K
Sbjct: 187 VNNFAAK 190

[Close]

BLAST of Cp4.1LG01g03680.1 vs. TrEMBL
Match: A0A0A0L671_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176250 PE=4 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 1.4e-63
Identity = 137/203 (67.49%), Postives = 164/203 (80.79%), Query Frame = 1


Query: 1   MEFQILKSLLTL---FFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLT 60
           ME QILKS LTL   FF +  TFT S V+SSSS  RPV+P+I+KACK TPYPRLCE +L+
Sbjct: 1   MESQILKSSLTLLIFFFIILTTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALS 60

Query: 61  LHASQTKRTQQEFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLQDSTD 120
           L+ASQTKR QQE CRA M SSLK  ++ATSIISKLSR+KMS YEA+VI DC+DNL+DS D
Sbjct: 61  LYASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYEAEVIGDCIDNLKDSVD 120

Query: 121 ELQRAIAAIKNLSRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVK 180
           EL+RA  AIK+LSR+KDV+FQL+SIKTWMSAA+TD +TC+DGLSGG+G KV   +K++VK
Sbjct: 121 ELRRASTAIKSLSRSKDVDFQLNSIKTWMSAAQTDVITCTDGLSGGSGWKVSKMLKKDVK 180

Query: 181 KSVAVVVRHISNALSLLNTFKYK 201
                VVR ISNAL L+N F YK
Sbjct: 181 NCSINVVRQISNALFLINNFNYK 203

[Close]

BLAST of Cp4.1LG01g03680.1 vs. TrEMBL
Match: B9R9Q8_RICCO (21 kDa protein, putative OS=Ricinus communis GN=RCOM_1500020 PE=4 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 3.7e-27
Identity = 74/192 (38.54%), Postives = 118/192 (61.46%), Query Frame = 1


Query: 9   LLTLFFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHASQTKRTQQ 68
           L+T   F+S T  +  + ++ +     K Y+K AC +T YP++C  +L+ + S  +    
Sbjct: 18  LITFLVFISNTQNSLAITTTKTSKTTYKNYLKTACNSTTYPKICYNTLSPYTSTIQTNDL 77

Query: 69  EFCRATMASSLKTTRDATSIISKLSRQK-MSTYEAKVIRDCVDNLQDSTDELQRAIAAIK 128
           + C A +  +LK   + ++++  LS+QK +S  E  VI+DC   ++DS DEL++++ A+K
Sbjct: 78  KLCNAALTITLKAASNTSAMVKSLSKQKGLSKGEVAVIKDCQYEIEDSVDELKQSLKALK 137

Query: 129 NLSRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSVAVVVRHI 188
           NL  + D+ FQ+ +IKTW+SAA TD  TC+DG     G KV SKVK ++KKS+  V R  
Sbjct: 138 NLKGSADMEFQIDNIKTWISAAITDENTCTDGFE---GMKVSSKVKSKIKKSIVNVNRLT 197

Query: 189 SNALSLLNTFKY 200
           SNAL+L+N   Y
Sbjct: 198 SNALALINKLSY 206

[Close]

BLAST of Cp4.1LG01g03680.1 vs. TrEMBL
Match: A0A161WV38_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_020198 PE=4 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 3.2e-26
Identity = 77/186 (41.40%), Postives = 115/186 (61.83%), Query Frame = 1


Query: 13  FFFVSITFTTSVV--ASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHASQTKRTQQEF 72
           F  +++ FT+ ++  AS++ V +  K +IK +C TT YP LC  +L  +AS  K      
Sbjct: 6   FAVITLFFTSCILHLASAAKVPQAYKTFIKTSCNTTTYPSLCNKNLLPYASSVKTDYIRL 65

Query: 73  CRATMASSLKTTRDATSIISKLSRQK-MSTYEAKVIRDCVDNLQDSTDELQRAIAAIKNL 132
           C A ++ ++K  +  + +++ L+ QK ++  EA  IRDC++N++DS DELQ+ + A+ NL
Sbjct: 66  CSAALSVTIKAAKKTSGLVTNLAEQKDLTEAEAAAIRDCIENIEDSLDELQQTVEAVANL 125

Query: 133 SRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSVAVVVRHISN 192
               D  FQL + KTW SAA TD  TC DG S   GR+V S VK +VKKS+  V +  SN
Sbjct: 126 -EGSDRAFQLSNAKTWASAAITDEDTCMDGFS---GREVSSGVKNKVKKSILAVTKLNSN 185

Query: 193 ALSLLN 196
           ALSL+N
Sbjct: 186 ALSLIN 187

[Close]

BLAST of Cp4.1LG01g03680.1 vs. TrEMBL
Match: A5BT23_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_005236 PE=4 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 9.2e-26
Identity = 80/195 (41.03%), Postives = 122/195 (62.56%), Query Frame = 1


Query: 3   FQILKSLL-TLFFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHAS 62
           F +L SL  TL  F SIT T+S   S+ S+T   K +IK +C +T YP +C  SL+ +AS
Sbjct: 12  FHVLSSLFFTLLLFNSITLTSSANTSTISLTIS-KQFIKTSCNSTTYPGVCYKSLSSYAS 71

Query: 63  QTKRTQQEFCRATMASSLKTTRDATSIISKL-SRQKMSTYEAKVIRDCVDNLQDSTDELQ 122
           + + +  +     +  SLK  R+A+S I+ L  R+ M+  E  +++DCV+N  D  DELQ
Sbjct: 72  KVQTSPLKLANVALTVSLKAARNASSTITLLLERKGMTRMETSIVKDCVENFGDCIDELQ 131

Query: 123 RAIAAIKNLSRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV 182
           +++   K+L   K+V FQ+ +IKTW+SAA TD  TCSDG     G+KV S ++Q++K  +
Sbjct: 132 QSLQEFKSLGGGKNVAFQMANIKTWVSAALTDEYTCSDGFE---GQKVSSSLQQQIKNYI 191

Query: 183 AVVVRHISNALSLLN 196
           + V +  SNAL+L+N
Sbjct: 192 SNVAKITSNALALIN 202

[Close]

BLAST of Cp4.1LG01g03680.1 vs. TrEMBL
Match: F6HAT8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_16s0022g00940 PE=4 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 9.2e-26
Identity = 80/195 (41.03%), Postives = 122/195 (62.56%), Query Frame = 1


Query: 3   FQILKSLL-TLFFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHAS 62
           F +L SL  TL  F SIT T+S   S+ S+T   K +IK +C +T YP +C  SL+ +AS
Sbjct: 12  FHVLSSLFFTLLLFNSITLTSSANTSTISLTIS-KQFIKTSCNSTTYPGVCYKSLSSYAS 71

Query: 63  QTKRTQQEFCRATMASSLKTTRDATSIISKL-SRQKMSTYEAKVIRDCVDNLQDSTDELQ 122
           + + +  +     +  SLK  R+A+S I+ L  R+ M+  E  +++DCV+N  D  DELQ
Sbjct: 72  KVQTSPLKLANVALTVSLKAARNASSTITLLLERKGMTRMETSIVKDCVENFGDCIDELQ 131

Query: 123 RAIAAIKNLSRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV 182
           +++   K+L   K+V FQ+ +IKTW+SAA TD  TCSDG     G+KV S ++Q++K  +
Sbjct: 132 QSLQEFKSLGGGKNVAFQMANIKTWVSAALTDEYTCSDGFE---GQKVSSSLQQQIKNYI 191

Query: 183 AVVVRHISNALSLLN 196
           + V +  SNAL+L+N
Sbjct: 192 SNVAKITSNALALIN 202

[Close]

BLAST of Cp4.1LG01g03680.1 vs. TAIR10
Match: AT5G62350.1 (AT5G62350.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 103.2 bits (256), Expect = 1.9e-22
Identity = 65/201 (32.34%), Postives = 111/201 (55.22%), Query Frame = 1


Query: 1   MEFQILKSLLTLFFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHA 60
           M  Q L  LL++ + +S+  T +  AS +  ++    +I+ +CKTT YP LC  SL+++A
Sbjct: 1   MAKQYLFVLLSISYLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYA 60

Query: 61  SQTKRTQQEFCRATMASSLKTTRDATSIISKLSRQK-MSTYEAKVIRDCVDNLQDSTDEL 120
           +  + + +      +A +L   +     +S+L+R K +   E + I+DCV+ + D+ D L
Sbjct: 61  NDIQTSPKRLAETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDRL 120

Query: 121 QRAIAAIKNLSRAKDVN---FQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEV 180
            +++  +K    AKD +   + + + +TW SAA TD  TCSDG S   GR +  ++K  V
Sbjct: 121 TKSVQELKLCGSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFS---GRVMDGRIKNSV 180

Query: 181 KKSVAVVVRHISNALSLLNTF 198
           +  +  V    SNALSL+N F
Sbjct: 181 RARIMNVGHETSNALSLINAF 198

[Close]

BLAST of Cp4.1LG01g03680.1 vs. TAIR10
Match: AT5G51520.1 (AT5G51520.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 98.2 bits (243), Expect = 6.1e-21
Identity = 63/205 (30.73%), Postives = 112/205 (54.63%), Query Frame = 1


Query: 4   QILKSLLTLFFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHASQT 63
           Q+L SL  +F F + T       S+SS ++  K ++K AC +T YP  C  SL+ ++S  
Sbjct: 3   QVLYSLTIVFVFFASTNIQKTSGSASSYSQNHKTFVKTACNSTTYPDKCYKSLSSYSSNI 62

Query: 64  KRTQQEFCRATMASSLKTTRDATSIISKLSRQKMSTY---------EAKVIRDCVDNLQD 123
           K    + C   +  ++K+ ++ATS++SKL +    +          EA +++DC++ ++D
Sbjct: 63  KSDPIKLCTTALNLNVKSAKEATSVVSKLLKMSQKSTAGRKGKMLPEALILKDCLEEMKD 122

Query: 124 STDELQRAIAAIKNLSRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQ 183
           +  EL++AI  +KNL     +   + +++TW+S+A TD  TC+DG       KV  + K+
Sbjct: 123 TIIELKQAITEMKNLQDGGSMAEHITNVRTWVSSALTDEGTCTDGFE---EVKVNKETKK 182

Query: 184 EVKKSVAVVVRHISNALSLLNTFKY 200
           +V K V  +    SN L+L+   +Y
Sbjct: 183 KVNKVVEELATTTSNTLALITNLRY 204

[Close]

BLAST of Cp4.1LG01g03680.1 vs. TAIR10
Match: AT4G25250.1 (AT4G25250.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 95.1 bits (235), Expect = 5.1e-20
Identity = 58/191 (30.37%), Postives = 111/191 (58.12%), Query Frame = 1


Query: 10  LTLFFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHASQTKRTQQE 69
           LTL  F++ +      A+     +    Y+K AC +T YP +C   L+ ++S  K    +
Sbjct: 9   LTLMVFINSSNFPKTAATPPGTYQNHTTYVKTACNSTTYPTMCYNCLSSYSSTIKSDPIK 68

Query: 70  FCRATMASSLKTTRDATSIISKLSRQKMS--TYEAKVIRDCVDNLQDSTDELQRAIAAIK 129
            C  ++  ++K+ ++AT ++S L ++  +  ++E  +++DCVD ++D+ DEL++A+A +K
Sbjct: 69  LCTTSLNLNVKSAKNATLVVSNLLQKAKAAKSHEVSILKDCVDEMKDTIDELKQAVAEMK 128

Query: 130 NL-SRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSVAVVVRH 189
            +    K     L ++KTW+S+A TD  TC+DG   G   +V  + K++VKK+++ + + 
Sbjct: 129 YVRGGGKTTEEHLKNVKTWVSSALTDEGTCTDGFEEG---RVNVETKKKVKKAISELSKT 188

Query: 190 ISNALSLLNTF 198
            SN L+LL  +
Sbjct: 189 TSNTLALLTHY 196

[Close]

BLAST of Cp4.1LG01g03680.1 vs. TAIR10
Match: AT5G62360.1 (AT5G62360.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 89.0 bits (219), Expect = 3.7e-18
Identity = 57/189 (30.16%), Postives = 101/189 (53.44%), Query Frame = 1


Query: 9   LLTLFFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHASQTKRTQQ 68
           L T    +++        ++++ T     ++K +C  T YPRLC  SL+ HAS  + + +
Sbjct: 14  LTTFLIIIAMLKLVHTTTTTTTTTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPK 73

Query: 69  EFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLQDSTDELQRAIAAIKN 128
               A +  +L + +  ++++ +LS  ++   E   +RDCV+ L D+ +EL+++I  +  
Sbjct: 74  LMAHAALNITLASAKVTSAMMVRLSNSRLKPKEVSAMRDCVEELGDTLEELRKSIGEMCQ 133

Query: 129 LSRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSVAVVVRHIS 188
           LS   +    +  I+TW+SAA TD  TC+DG     G  +  KVK  V+  + V+    S
Sbjct: 134 LS-GSNYEVYISDIQTWVSAALTDVNTCTDGFE---GEDMDGKVKVLVRGRILVIAHLTS 193

Query: 189 NALSLLNTF 198
           NAL+L+N F
Sbjct: 194 NALALINHF 198

[Close]

BLAST of Cp4.1LG01g03680.1 vs. TAIR10
Match: AT3G47380.1 (AT3G47380.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 81.3 bits (199), Expect = 7.7e-16
Identity = 60/200 (30.00%), Postives = 103/200 (51.50%), Query Frame = 1


Query: 11  TLFFFVSITFTTSVVASSSSVTRPVKP------YIKKACKTTPYPRLCEISLTLHASQTK 70
           TLF F+    +T+++ +SSS  R          +I+ +CK T YP +C  SLT +A+  +
Sbjct: 8   TLFLFL---LSTAILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQ 67

Query: 71  RTQQEFCRATMASSLKTTRDATSIISKLSR-QKMSTYEAKVIRDCVDNLQDSTDELQRAI 130
            + +      +  ++   +     + +L R   +   E + ++DC++ + D+ D L  +I
Sbjct: 68  TSPRRLAETALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSI 127

Query: 131 AAIKNLSRAKDVN---FQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV 190
             +K    AK  +   F + + +TW SAA T+A TCSDG    AGR +  +VK  V+  +
Sbjct: 128 HEVKMCGSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGF---AGRVMDGRVKNSVRARI 187

Query: 191 AVVVRHISNALSLLNTFKYK 201
             + R  SNAL+L+N F  K
Sbjct: 188 LNLGRGTSNALALINAFAKK 201

[Close]

BLAST of Cp4.1LG01g03680.1 vs. NCBI nr
Match: gi|659077238|ref|XP_008439099.1| (PREDICTED: 21 kDa protein-like [Cucumis melo])

HSP 1 Score: 255.8 bits (652), Expect = 6.5e-65
Identity = 142/202 (70.30%), Postives = 164/202 (81.19%), Query Frame = 1


Query: 1   MEFQILKSLLTLFFFVSI--TFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTL 60
           ME QILKS LTLFFF  I  TFT S VASSSS  RPVKP+I+KACK TPYPRLCE +L+L
Sbjct: 1   MESQILKSSLTLFFFFIILTTFTPSAVASSSSTVRPVKPHIRKACKPTPYPRLCETALSL 60

Query: 61  HASQTKRTQQEFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLQDSTDE 120
           +ASQTKR QQE CRA M SSLK  ++ATSIISKLSR+KMS YE +VI DC+D+L+DS DE
Sbjct: 61  YASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYETEVIGDCIDDLKDSVDE 120

Query: 121 LQRAIAAIKNLSRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKK 180
           L+RA  AIK+LSR+KDV+FQL+SIKTW SAA TD +TC+DGLSGG+G KV  KVK+EVK 
Sbjct: 121 LRRASTAIKSLSRSKDVDFQLNSIKTWTSAALTDIITCTDGLSGGSGWKVNKKVKKEVKN 180

Query: 181 SVAVVVRHISNALSLLNTFKYK 201
               VVR ISNALSL+N F YK
Sbjct: 181 CSINVVRQISNALSLINNFNYK 202

[Close]

BLAST of Cp4.1LG01g03680.1 vs. NCBI nr
Match: gi|449460810|ref|XP_004148137.1| (PREDICTED: 21 kDa protein-like [Cucumis sativus])

HSP 1 Score: 250.8 bits (639), Expect = 2.1e-63
Identity = 137/203 (67.49%), Postives = 164/203 (80.79%), Query Frame = 1


Query: 1   MEFQILKSLLTL---FFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLT 60
           ME QILKS LTL   FF +  TFT S V+SSSS  RPV+P+I+KACK TPYPRLCE +L+
Sbjct: 1   MESQILKSSLTLLIFFFIILTTFTPSAVSSSSSTVRPVQPHIRKACKPTPYPRLCETALS 60

Query: 61  LHASQTKRTQQEFCRATMASSLKTTRDATSIISKLSRQKMSTYEAKVIRDCVDNLQDSTD 120
           L+ASQTKR QQE CRA M SSLK  ++ATSIISKLSR+KMS YEA+VI DC+DNL+DS D
Sbjct: 61  LYASQTKRNQQELCRAAMVSSLKAAQNATSIISKLSRRKMSAYEAEVIGDCIDNLKDSVD 120

Query: 121 ELQRAIAAIKNLSRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVK 180
           EL+RA  AIK+LSR+KDV+FQL+SIKTWMSAA+TD +TC+DGLSGG+G KV   +K++VK
Sbjct: 121 ELRRASTAIKSLSRSKDVDFQLNSIKTWMSAAQTDVITCTDGLSGGSGWKVSKMLKKDVK 180

Query: 181 KSVAVVVRHISNALSLLNTFKYK 201
                VVR ISNAL L+N F YK
Sbjct: 181 NCSINVVRQISNALFLINNFNYK 203

[Close]

BLAST of Cp4.1LG01g03680.1 vs. NCBI nr
Match: gi|255539737|ref|XP_002510933.1| (PREDICTED: 21 kDa protein [Ricinus communis])

HSP 1 Score: 129.8 bits (325), Expect = 5.3e-27
Identity = 74/192 (38.54%), Postives = 118/192 (61.46%), Query Frame = 1


Query: 9   LLTLFFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHASQTKRTQQ 68
           L+T   F+S T  +  + ++ +     K Y+K AC +T YP++C  +L+ + S  +    
Sbjct: 18  LITFLVFISNTQNSLAITTTKTSKTTYKNYLKTACNSTTYPKICYNTLSPYTSTIQTNDL 77

Query: 69  EFCRATMASSLKTTRDATSIISKLSRQK-MSTYEAKVIRDCVDNLQDSTDELQRAIAAIK 128
           + C A +  +LK   + ++++  LS+QK +S  E  VI+DC   ++DS DEL++++ A+K
Sbjct: 78  KLCNAALTITLKAASNTSAMVKSLSKQKGLSKGEVAVIKDCQYEIEDSVDELKQSLKALK 137

Query: 129 NLSRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSVAVVVRHI 188
           NL  + D+ FQ+ +IKTW+SAA TD  TC+DG     G KV SKVK ++KKS+  V R  
Sbjct: 138 NLKGSADMEFQIDNIKTWISAAITDENTCTDGFE---GMKVSSKVKSKIKKSIVNVNRLT 197

Query: 189 SNALSLLNTFKY 200
           SNAL+L+N   Y
Sbjct: 198 SNALALINKLSY 206

[Close]

BLAST of Cp4.1LG01g03680.1 vs. NCBI nr
Match: gi|1021034653|gb|KZM92437.1| (hypothetical protein DCAR_020198 [Daucus carota subsp. sativus])

HSP 1 Score: 126.7 bits (317), Expect = 4.5e-26
Identity = 77/186 (41.40%), Postives = 115/186 (61.83%), Query Frame = 1


Query: 13  FFFVSITFTTSVV--ASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHASQTKRTQQEF 72
           F  +++ FT+ ++  AS++ V +  K +IK +C TT YP LC  +L  +AS  K      
Sbjct: 6   FAVITLFFTSCILHLASAAKVPQAYKTFIKTSCNTTTYPSLCNKNLLPYASSVKTDYIRL 65

Query: 73  CRATMASSLKTTRDATSIISKLSRQK-MSTYEAKVIRDCVDNLQDSTDELQRAIAAIKNL 132
           C A ++ ++K  +  + +++ L+ QK ++  EA  IRDC++N++DS DELQ+ + A+ NL
Sbjct: 66  CSAALSVTIKAAKKTSGLVTNLAEQKDLTEAEAAAIRDCIENIEDSLDELQQTVEAVANL 125

Query: 133 SRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSVAVVVRHISN 192
               D  FQL + KTW SAA TD  TC DG S   GR+V S VK +VKKS+  V +  SN
Sbjct: 126 -EGSDRAFQLSNAKTWASAAITDEDTCMDGFS---GREVSSGVKNKVKKSILAVTKLNSN 185

Query: 193 ALSLLN 196
           ALSL+N
Sbjct: 186 ALSLIN 187

[Close]

BLAST of Cp4.1LG01g03680.1 vs. NCBI nr
Match: gi|147860755|emb|CAN79278.1| (hypothetical protein VITISV_005236 [Vitis vinifera])

HSP 1 Score: 125.2 bits (313), Expect = 1.3e-25
Identity = 80/195 (41.03%), Postives = 122/195 (62.56%), Query Frame = 1


Query: 3   FQILKSLL-TLFFFVSITFTTSVVASSSSVTRPVKPYIKKACKTTPYPRLCEISLTLHAS 62
           F +L SL  TL  F SIT T+S   S+ S+T   K +IK +C +T YP +C  SL+ +AS
Sbjct: 12  FHVLSSLFFTLLLFNSITLTSSANTSTISLTIS-KQFIKTSCNSTTYPGVCYKSLSSYAS 71

Query: 63  QTKRTQQEFCRATMASSLKTTRDATSIISKL-SRQKMSTYEAKVIRDCVDNLQDSTDELQ 122
           + + +  +     +  SLK  R+A+S I+ L  R+ M+  E  +++DCV+N  D  DELQ
Sbjct: 72  KVQTSPLKLANVALTVSLKAARNASSTITLLLERKGMTRMETSIVKDCVENFGDCIDELQ 131

Query: 123 RAIAAIKNLSRAKDVNFQLHSIKTWMSAAETDAMTCSDGLSGGAGRKVRSKVKQEVKKSV 182
           +++   K+L   K+V FQ+ +IKTW+SAA TD  TCSDG     G+KV S ++Q++K  +
Sbjct: 132 QSLQEFKSLGGGKNVAFQMANIKTWVSAALTDEYTCSDGFE---GQKVSSSLQQQIKNYI 191

Query: 183 AVVVRHISNALSLLN 196
           + V +  SNAL+L+N
Sbjct: 192 SNVAKITSNALALIN 202

The following BLAST results are available for this feature:

BLAST of Cp4.1LG01g03680.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
21KD_DAUCA	1.4e-22	34.22	21 kDa protein OS=Daucus carota PE=2 SV=1	[more]

BLAST of Cp4.1LG01g03680.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L671_CUCSA	1.4e-63	67.49	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176250 PE=4 SV=1	[more]
B9R9Q8_RICCO	3.7e-27	38.54	21 kDa protein, putative OS=Ricinus communis GN=RCOM_1500020 PE=4 SV=1	[more]
A0A161WV38_DAUCA	3.2e-26	41.40	Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_020198 PE=4 SV=1	[more]
A5BT23_VITVI	9.2e-26	41.03	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_005236 PE=4 SV=1	[more]
F6HAT8_VITVI	9.2e-26	41.03	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_16s0022g00940 PE=4 SV=...	[more]

BLAST of Cp4.1LG01g03680.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G62350.1	1.9e-22	32.34	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G51520.1	6.1e-21	30.73	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25250.1	5.1e-20	30.37	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62360.1	3.7e-18	30.16	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G47380.1	7.7e-16	30.00	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

BLAST of Cp4.1LG01g03680.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659077238\|ref\|XP_008439099.1\|	6.5e-65	70.30	PREDICTED: 21 kDa protein-like [Cucumis melo]	[more]
gi\|449460810\|ref\|XP_004148137.1\|	2.1e-63	67.49	PREDICTED: 21 kDa protein-like [Cucumis sativus]	[more]
gi\|255539737\|ref\|XP_002510933.1\|	5.3e-27	38.54	PREDICTED: 21 kDa protein [Ricinus communis]	[more]
gi\|1021034653\|gb\|KZM92437.1\|	4.5e-26	41.40	hypothetical protein DCAR_020198 [Daucus carota subsp. sativus]	[more]
gi\|147860755\|emb\|CAN79278.1\|	1.3e-25	41.03	hypothetical protein VITISV_005236 [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004857	enzyme inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR006501	Pectinesterase_inhib_dom

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cp4.1LG01g03680	Cp4.1LG01g03680	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG01g03680.1:cds:001	Cp4.1LG01g03680.1:cds:001	CDS
Cp4.1LG01g03680.1:cds:002	Cp4.1LG01g03680.1:cds:002	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG01g03680.1	Cp4.1LG01g03680.1-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	GENE3D	G3DSA:1.20.140.40		coord: 37..197 score: 4.6
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 38..193 score: 1.0
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 34..193 score: 1.7
IPR006501	Pectinesterase inhibitor domain	TIGRFAMs	TIGR01614	TIGR01614	coord: 12..196 score: 2.3
IPR006501	Pectinesterase inhibitor domain	unknown	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 37..197 score: 1.96
None	No IPR available	unknown	Coil	Coil	coord: 109..132 scor
None	No IPR available	PANTHER	PTHR31080	FAMILY NOT NAMED	coord: 1..195 score: 1.0
None	No IPR available	PANTHER	PTHR31080:SF15	SUBFAMILY NOT NAMED	coord: 1..195 score: 1.0