CmoCh15G014850.1 (mRNA) Cucurbita moschata (Rifu)

BLAST of CmoCh15G014850.1 vs. Swiss-Prot
Match: C80B2_COPJA (Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 6.2e-111
Identity = 221/487 (45.38%), Postives = 296/487 (60.78%), Query Frame = 1

Query: 3   IASFSFLLLLLPLIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHI 62
           I SFSFLL L    F ILR     S+   LPPGP+P PI+GNLLQ+G   H    + +  
Sbjct: 8   IVSFSFLLFL----FFILR----DSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQK 67

Query: 63  YGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPEC 122
           YG L SLKLG+Q +VVASSPAAAA ILKT D++LS RY+FQ    +   +N S+++S EC
Sbjct: 68  YGELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKEHVEN-SIVWS-EC 127

Query: 123 GDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVFNT 182
            D WK LR +CR +LFT + IESQ+ +R  K  EMV+FL+ K+G VV+I E+VF T+ N 
Sbjct: 128 NDNWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNI 187

Query: 183 LSNFIFSRDLVGFVGD--GFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAI 242
             N IFS+D+        G   +K     ++D+G   N AD++P++   DL G R+  A 
Sbjct: 188 FGNLIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAE 247

Query: 243 YKNQVDSSWEILIKERR-TIHSQG-----------------------EVLSAGTDTTTTT 302
              Q+   W +++KERR T  S+                        E+  AGT+T+ +T
Sbjct: 248 VLQQIYDVWGVMLKERRGTKGSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSAST 307

Query: 303 VEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLL 362
           +EWA+ EL K   +++++R E    +    + ES L  L YL   VKETLRLHPP P LL
Sbjct: 308 IEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPPTPLLL 367

Query: 363 PRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRF 422
           PR A E C +M Y++PK   I VN W IGRDP  W+DP +FKPERF   D D KG+D+  
Sbjct: 368 PRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFEL 427

Query: 423 LPFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPL 464
           +PFGGGRRICPGLP+A+    LI+ATL+ NFEW LP     S L ++ + G+TLQK+ PL
Sbjct: 428 IPFGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPL 484

BLAST of CmoCh15G014850.1 vs. Swiss-Prot
Match: C80B2_ESCCA ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 5.4e-107
Identity = 205/492 (41.67%), Postives = 293/492 (59.55%), Query Frame = 1

Query: 1   MEIASFSFLLLLLPLIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFS 60
           ME+ + + + +++  I  +L     SS    LPPGPKPWPI+GNLLQ+G+  H    + +
Sbjct: 1   MEVVTVALIAVIISSILYLL---FGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELA 60

Query: 61  HIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSP 120
             YG + +LK+G + +VVAS+ +AA+ ILKT DR+LSARY+FQ    +   +N S+++S 
Sbjct: 61  QTYGDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVEN-SIVWS- 120

Query: 121 ECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVF 180
           +C + WKNLR +CR +LFT + IESQA +R KK  EMVE+L  KQG  V+I E++F T+ 
Sbjct: 121 DCTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLV 180

Query: 181 NTLSNFIFSRDL--VGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKA 240
           N   N IFS+++  +G    G +  K     M++LG   N AD++P+L  FDL G RK+ 
Sbjct: 181 NIFGNLIFSQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEV 240

Query: 241 AIYKNQVDSSWEILIKERRTIHSQG---------------------------EVLSAGTD 300
           A     + + W  +++ER+                                 E+  AGT+
Sbjct: 241 AECLKGIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTE 300

Query: 301 TTTTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPP 360
           T+ +T+EWAM EL KN  I  ++R E +  +   ++ ES    L YL   VKETLRLHPP
Sbjct: 301 TSASTIEWAMTELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETLRLHPP 360

Query: 361 APFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKG 420
            P LLPR A E C ++ Y++PK   I VN WGIGRDP  W DP +F PERF     D +G
Sbjct: 361 TPLLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDFRG 420

Query: 421 HDYRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQ 464
           +D+  +PFG GRRICPGLP+A   + L++AT + N +WCLPN      L +  + G+TLQ
Sbjct: 421 NDFSLIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQ 480

BLAST of CmoCh15G014850.1 vs. Swiss-Prot
Match: C80B1_ESCCA ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica GN=CYP80B1 PE=2 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 5.4e-107
Identity = 205/486 (42.18%), Postives = 289/486 (59.47%), Query Frame = 1

Query: 7   SFLLLLLPLIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPL 66
           S + L+  +I  IL  +   S    LPPGPKPWPI+GNLLQ+G+  H    + +  YG +
Sbjct: 3   STVALIAVIISSILYLLFGGSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDI 62

Query: 67  ISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRW 126
            +LK+G + +VVAS+ +AA+ ILKT DR+LSARY+FQ    +   +N S+++S +C + W
Sbjct: 63  FTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVEN-SIVWS-DCTETW 122

Query: 127 KNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVFNTLSNF 186
           KNLR +CR +LFT + IESQA +R KK  EMVE+L  KQG  V+I E++F T+ N   N 
Sbjct: 123 KNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNL 182

Query: 187 IFSRDL--VGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQ 246
           IFS+++  +G    G +  K     M++LG   N AD++P+L  FDL G RK+ A     
Sbjct: 183 IFSQNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKG 242

Query: 247 VDSSWEILIKERRTIHSQG---------------------------EVLSAGTDTTTTTV 306
           + + W  +++ER+                                 E+  AGT+T+ +T+
Sbjct: 243 IYAIWGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTI 302

Query: 307 EWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLP 366
           EWAM EL KN  I  ++R E +  +   ++ ES    L YL   VKETLRLHPP P LLP
Sbjct: 303 EWAMTELTKNPKITAKIRSEIQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLP 362

Query: 367 RYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFL 426
           R A E C ++ Y++PK   I VN WGIGRDP  W DP +F PERF     D +G+D+  +
Sbjct: 363 RRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPERFLNSSVDFRGNDFSLI 422

Query: 427 PFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPLK 464
           PFG GRRICPGLP+A   + L++AT + N +WCLPN      L +  + G+TLQKE PL 
Sbjct: 423 PFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLF 482

BLAST of CmoCh15G014850.1 vs. Swiss-Prot
Match: C80B3_PAPSO ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum GN=CYP80B3 PE=1 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 9.6e-104
Identity = 203/482 (42.12%), Postives = 280/482 (58.09%), Query Frame = 1

Query: 11  LLLPLIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPLISLK 70
           L+  +I   L  I   S    LPPGPKPWPI+GNLLQ+G+  H    Q +  YG L SLK
Sbjct: 2   LVAVVITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSLK 61

Query: 71  LGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWKNLR 130
           LG++ +VVAS+P AA+ ILKT DR+LS RY+FQ    +   +N S+++S EC + WK LR
Sbjct: 62  LGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVEN-SIVWS-ECNETWKKLR 121

Query: 131 SICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVFNTLSNFIFSR 190
            +CR +LFT + IESQA +R  K  EMVE+L+   G+ V+I E+VF T+ N   N IFS+
Sbjct: 122 KVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQ 181

Query: 191 DL--VGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQVDSS 250
           ++  +G    G   +K     M++LG   N AD++P L  FDL G  K  A     + S 
Sbjct: 182 NIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSV 241

Query: 251 WEILIKERRTIHSQG--------------------------EVLSAGTDTTTTTVEWAMA 310
           W  ++KER+                                E+  AGT+T+ +T+EWA++
Sbjct: 242 WGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALS 301

Query: 311 ELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAPE 370
           EL KN  +   +R E    +    + ES +  + YL   VKETLRLHP  P LLPR A E
Sbjct: 302 ELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALE 361

Query: 371 ACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFLPFGGG 430
            C+++ Y++PK   I VN WGIGRDP  W DP  F PERF     D KG+D+  +PFG G
Sbjct: 362 TCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPFGAG 421

Query: 431 RRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPLKLLSKN 465
           RRICPG+P+A   + LI+++L+ NF+W  P   D S L +  + G+TLQKE PL ++ K 
Sbjct: 422 RRICPGVPLATQFISLIVSSLVQNFDWGFPKGMDPSQLIMEEKFGLTLQKEPPLYIVPKT 481

BLAST of CmoCh15G014850.1 vs. Swiss-Prot
Match: C80BX_PAPSO ((S)-N-methylcoclaurine 3'-hydroxylase-like protein OS=Papaver somniferum GN=CYP80BX PE=2 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 1.0e-89
Identity = 181/440 (41.14%), Postives = 252/440 (57.27%), Query Frame = 1

Query: 1   MEIASFSFLLLLLP-LIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQF 60
           MEI + + + ++    +++I+R  S +     LPPGPKPWPI+GNLLQ+G+  H    Q 
Sbjct: 1   MEIVTVALIAIVFTTFLYLIVRESSPNG----LPPGPKPWPIVGNLLQLGEKPHSQFAQL 60

Query: 61  SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFS 120
           +  YG L +LKLG Q +VVAS+P AA+ +LK  DR L  RY+FQ    +   +N S++++
Sbjct: 61  AETYGDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKNHVEN-SIVWN 120

Query: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITV 180
            EC + WK LR +CR +LFT + IE+QA +R  K  EMV++L+  +G  V+I E++F T+
Sbjct: 121 -ECNETWKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTL 180

Query: 181 FNTLSNFIFSRDL--VGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKK 240
            N   N IFS+D+  +G    G   +K     M++LG   N  D+ PLL   DL G RK 
Sbjct: 181 VNMFGNLIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPGDYVPLLGSLDLFGQRKD 240

Query: 241 AAIYKNQVDSSWEILIKERRTIHSQG---------------------------EVLSAGT 300
            A     V   W  ++KERR    Q                            ++ SAGT
Sbjct: 241 VADCLQGVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQQINSLLLDMFSAGT 300

Query: 301 DTTTTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHP 360
           +TT +T+EWA+ EL KN  +   +R E    +   A+ ES +  L YL   VKETLRLHP
Sbjct: 301 ETTASTIEWALTELTKNPQVTADIRSELLSVVGKRAVKESDILNLPYLQAFVKETLRLHP 360

Query: 361 PAPFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLK 411
           P P L+PR A E C+++ Y++PK   I VN WGIGRDP  W DP  F P+RF     D K
Sbjct: 361 PTPLLIPRRALETCQVLNYTIPKECQIMVNAWGIGRDPKTWTDPLKFSPDRFLNSSIDFK 420

BLAST of CmoCh15G014850.1 vs. TrEMBL
Match: B9RHX8_RICCO ((S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative OS=Ricinus communis GN=RCOM_1574420 PE=3 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 1.7e-139
Identity = 257/482 (53.32%), Postives = 336/482 (69.71%), Query Frame = 1

Query: 11  LLLPLIFIILRHISSS--SKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPLIS 70
           LLLPLI II+  I SS  SK+ PLPPGP+ WPILGN+LQ+G+N HISM  ++ ++GPLIS
Sbjct: 14  LLLPLILIIVMQILSSISSKRRPLPPGPRQWPILGNILQVGKNPHISMANYAKVHGPLIS 73

Query: 71  LKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTP-DRPLHDNCSLLFSPECGDRWK 130
           L+LG + +VVASSP AAA ILKT DRLLS RYI   TP +  + D  +L+++P C D+WK
Sbjct: 74  LRLGTRVVVVASSPTAAAEILKTHDRLLSGRYIPATTPYEDNVLDRIALVWNPSCSDQWK 133

Query: 131 NLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 190
            LR++CR++LF+A+AIESQA+LR KK+ EM++FL SKQG +V I E+VF T FNT+SN +
Sbjct: 134 FLRAMCRSELFSAKAIESQATLREKKLTEMLDFLTSKQGQIVNIGEVVFTTAFNTISNLL 193

Query: 191 FSRDLVGFVGDGFNG-IKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQVD 250
           FS+DL+ F   G  G +K     +++L   PN+ADFYP+L   D QG+++K      ++ 
Sbjct: 194 FSKDLLSFEDQGNAGELKTLISTLMELATCPNIADFYPVLTKLDPQGIKRKMKNCLERMF 253

Query: 251 SSWEILIKERRTIH--------------SQG-----------EVLSAGTDTTTTTVEWAM 310
             W+I IKERR  H              S G           E+ SAG DTTTTTVEWAM
Sbjct: 254 GVWDIYIKERRERHVKDARKTDFLDVFLSNGFDDHQINWLLLELFSAGADTTTTTVEWAM 313

Query: 311 AELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAP 370
           AELLK   ++ +VREE +  I    I ES + +L YL  CVKETLRLHPP PFL+PR AP
Sbjct: 314 AELLKEITVLEKVREELETEIGKDMIRESHIPQLKYLNACVKETLRLHPPVPFLIPRRAP 373

Query: 371 EACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFLPFGG 430
           EACE+M Y++PKH+ I VNVW IGRDPS WEDP S++PERF   + D KGH++ FLPFG 
Sbjct: 374 EACEVMNYTIPKHSQIIVNVWAIGRDPSAWEDPLSYRPERFLDSNLDFKGHNFEFLPFGS 433

Query: 431 GRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPLKLLSK 464
           GRRICPGLPM   Q+PLILA+L+H F+W L N +D + LD+N +  +TL+KE+ L ++ K
Sbjct: 434 GRRICPGLPMGTRQLPLILASLVHCFDWSLQNGDDPAMLDMNDKFSITLEKEQHLLVVPK 493

BLAST of CmoCh15G014850.1 vs. TrEMBL
Match: B9R9F5_RICCO ((S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative OS=Ricinus communis GN=RCOM_1496790 PE=3 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 1.6e-137
Identity = 250/484 (51.65%), Postives = 333/484 (68.80%), Query Frame = 1

Query: 10  LLLLPLIFIILRHI----SSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGP 69
           LL LP++ +IL  I    SS  +  PLPPGPKPWPILGN+LQ+ +  HI +  F+ +YGP
Sbjct: 10  LLPLPILVVILTQIISFISSRRQHLPLPPGPKPWPILGNILQVDKKLHICIANFAKVYGP 69

Query: 70  LISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTP-DRPLHDNCSLLFSPECGD 129
           LISL+LG Q LVVAS+P++AA +LK  DRLLSAR+I +  P +  + +  +++++P C D
Sbjct: 70  LISLRLGNQVLVVASTPSSAAEVLKNHDRLLSARFILKAIPSESHILERVAIVWNPACND 129

Query: 130 RWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVFNTLS 189
            WK+LR++CR +LF+ +AIESQA LR KK+ EM+EFL +KQG  V + E+VF T+FNT+S
Sbjct: 130 HWKSLRALCRTELFSPKAIESQAILREKKLAEMLEFLITKQGQAVNVAEVVFGTIFNTIS 189

Query: 190 NFIFSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQ 249
           + +FS DL+GF      G+K    +M+++   PN+A+FYP+L   D QGL++K      +
Sbjct: 190 HLLFSTDLIGFENQ-IGGVKSLLWSMMEMATSPNIAEFYPILAPLDPQGLKRKMTKCLKE 249

Query: 250 VDSSWEILIKERRTIH----------------SQG-----------EVLSAGTDTTTTTV 309
           +   WEI IKERR  H                S G           E+LSAGTDTT+T+V
Sbjct: 250 MFGVWEIYIKERRRTHDHDHAAPKTDFLDIFLSNGFDDDQINWLVMELLSAGTDTTSTSV 309

Query: 310 EWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLP 369
           EWAMAELLKN++ M +VREE  + IN   I ES +S+L YL  CVKETLRLHPPAPFL+P
Sbjct: 310 EWAMAELLKNKEAMKKVREELDREINKNPIKESHVSQLPYLNACVKETLRLHPPAPFLIP 369

Query: 370 RYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFL 429
           R A E CE+M Y++PK + + VNVW IGRDPS+WEDP SFKPERF G   D+KGHD+  +
Sbjct: 370 RRATENCEVMNYTIPKDSQVLVNVWAIGRDPSVWEDPSSFKPERFLGSSLDVKGHDFELI 429

Query: 430 PFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPLK 462
           PFG GRRICPGLPMA  Q+ L+LA+LIH F+W LPN  D + +D+  + G+TLQ E PL 
Sbjct: 430 PFGSGRRICPGLPMATRQLSLVLASLIHCFDWSLPNGGDPAKVDMTEKFGITLQMEHPLL 489

BLAST of CmoCh15G014850.1 vs. TrEMBL
Match: B9I4C8_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0012s13580g PE=3 SV=1)

HSP 1 Score: 473.4 bits (1217), Expect = 3.2e-130
Identity = 247/482 (51.24%), Postives = 322/482 (66.80%), Query Frame = 1

Query: 10  LLLLPLIFIILRHI-SSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPLIS 69
           LLLLPL FIIL++I  S S+   LPPGPKPWPI+GNLL +G   H+S+ + + I+GPLIS
Sbjct: 21  LLLLPLTFIILKNIIRSCSESKYLPPGPKPWPIIGNLLHVGNQPHVSLAEIAKIHGPLIS 80

Query: 70  LKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTP-DRPLHDNCSLLFSPECGDRWK 129
           L+LG Q LVV SS  AAA ILKT DR LSAR++ Q+ P +  +    +L++ PE  D WK
Sbjct: 81  LRLGTQLLVVGSSAKAAAEILKTHDRFLSARHVPQVIPRESHVLRRVALVWCPESIDTWK 140

Query: 130 NLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 189
            LR +CR +LF+A+AIES A+LR KK+ E+++FL +++G VV I E+VF TVFNT+SN +
Sbjct: 141 LLRGLCRTELFSAKAIESSATLREKKVGELMDFLVAREGKVVSIGEVVFSTVFNTISNLL 200

Query: 190 FSRDLVGFVGDGFN-GIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQVD 249
           FS DL G    G + G+K   + ++ L   PN+ADFYP+    D QGLR+K +    +  
Sbjct: 201 FSNDLAGLEEKGMSSGLKSHVRKLMLLVATPNIADFYPIFAGLDPQGLRRKLSKLVEETF 260

Query: 250 SSWEILIKERRT--IHSQ-----------------------GEVLSAGTDTTTTTVEWAM 309
           + W I IKERR   +H                          E+ SAGTDTT TT+EWA+
Sbjct: 261 AIWAINIKERRNSYVHDSPKRDFLDVFLANGFDDDQINWLAAELFSAGTDTTATTIEWAV 320

Query: 310 AELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAP 369
           AE+LKN+++M +V EE ++ I    I ES +S L YL  C+KETLRLHPP P L+P  A 
Sbjct: 321 AEILKNKEVMKKVDEELEREITKNTISESDVSGLPYLNACIKETLRLHPPVPLLVPHRAT 380

Query: 370 EACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFLPFGG 429
           E CE+M Y++PK + + VNVW I RDPS WEDP SFKP+RF G + + KG +Y FLPFG 
Sbjct: 381 ETCEVMKYTIPKDSQVLVNVWAISRDPSTWEDPLSFKPDRFLGSNLEFKGGNYEFLPFGA 440

Query: 430 GRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPLKLLSK 464
           GRRICPGLPMA   VPLILA+LI  F+W LPN ED + LD+  + GV LQKE+PL L+ K
Sbjct: 441 GRRICPGLPMANKLVPLILASLIRCFDWSLPNGEDLAKLDMKDKFGVVLQKEQPLVLVPK 500

BLAST of CmoCh15G014850.1 vs. TrEMBL
Match: A0A067KEE9_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_11052 PE=3 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 2.7e-129
Identity = 241/483 (49.90%), Postives = 322/483 (66.67%), Query Frame = 1

Query: 10  LLLLPLIFIILRHIS-SSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPLIS 69
           L+ LP++FI  + I    SKQ  LPPGP+PWPI+GN+  IG+  HIS+T  + ++GPLIS
Sbjct: 11  LVFLPIVFIFFKKICFPPSKQCRLPPGPRPWPIVGNIPHIGKKLHISLTNIAKVHGPLIS 70

Query: 70  LKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRP-LHDNCSLLFSPECGDRWK 129
           L+LG Q ++V SS  AA  ILK  DRLLSAR I +  P +  + +  +L+++P C D+WK
Sbjct: 71  LRLGTQLVIVGSSSKAAIEILKHNDRLLSARSIPKAVPRKSHVLERLALVWNPTCNDQWK 130

Query: 130 NLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 189
           + R++CR +LF+A+A+E+QA+LR KKM EM+E+L S++G  V I E+VF TVFNT+SN +
Sbjct: 131 SFRTLCRTELFSAKAVEAQAALREKKMTEMIEYLSSQEGEAVNIGEIVFATVFNTISNLL 190

Query: 190 FSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQVDS 249
           FS+D +     G   +K     MI+L   PN+ADFYP+    DLQG+R++ A    ++  
Sbjct: 191 FSKDFMSLQNQG---LKSLLWRMIELAAAPNVADFYPIFVELDLQGIRRQMAKCLKEMFG 250

Query: 250 SWEILIKERRTIHSQG---------------------------EVLSAGTDTTTTTVEWA 309
             EI IKERR  H+                             E+ SAG DTTTTTVEWA
Sbjct: 251 ICEIYIKERRERHANAHNASSTDFLDVFLSNGLDDDQINWLFMELFSAGVDTTTTTVEWA 310

Query: 310 MAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYA 369
           +AELLKN+  M  +++E +  IN   I ES++S+L YL  CVKETLRLHPPAPFLLP  A
Sbjct: 311 IAELLKNKQAMKTLQQELETKINRNPIQESQVSQLLYLNACVKETLRLHPPAPFLLPHCA 370

Query: 370 PEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFLPFG 429
            EACELM + VPK+T + VNVW I RDP IWED + FKPERF     D K  ++ FLPFG
Sbjct: 371 TEACELMNFVVPKNTRVLVNVWAIARDPLIWEDSELFKPERFLHSGLDFKSQNFDFLPFG 430

Query: 430 GGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPLKLLS 464
            GRRICPGLPMA  Q+P+ILA+LIH+F+W LPN ED + LD+  + G+TLQKE+PL ++ 
Sbjct: 431 SGRRICPGLPMAIRQLPMILASLIHHFDWSLPNGEDPALLDMTDKFGITLQKEQPLFIVP 490

BLAST of CmoCh15G014850.1 vs. TrEMBL
Match: M1B4Q0_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400014274 PE=3 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 4.6e-129
Identity = 240/475 (50.53%), Postives = 326/475 (68.63%), Query Frame = 1

Query: 9   LLLLLPLIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPLIS 68
           LL L+P  F+I++ +   S    LPPGP+PWP+LGN+LQ+G+N HIS+ +F+ I+GPLIS
Sbjct: 10  LLFLIPFFFLIVKQLIPKSGN--LPPGPRPWPVLGNILQLGKNPHISLAKFAEIHGPLIS 69

Query: 69  LKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTP-DRPLHDNCSLLFSPECGDRWK 128
           +KLG Q +VVASSP +AA ILKTQDRLLSAR    + P +  + D  S++FS + G+ WK
Sbjct: 70  VKLGTQLVVVASSPHSAAEILKTQDRLLSARSPPSVVPYELSVIDQHSIVFSSDLGNHWK 129

Query: 129 NLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 188
            LR+ CR  LF+ +A+ESQA+LR KK++EM+ FL+SK+G  V+I E++F T+ NTL N  
Sbjct: 130 FLRAFCRTHLFSPKAVESQAALRDKKVSEMIHFLRSKKGETVKISEILFGTILNTLGNLF 189

Query: 189 FSRDLVGFVGDGFN--GIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQV 248
           FS+DLV  +    N  GIK  F+  I+LG +PN++DFYPL    DLQGLRK+  IY++++
Sbjct: 190 FSKDLVCGLDYERNPSGIKRVFRKYIELGAMPNISDFYPLFEALDLQGLRKQTEIYQSRL 249

Query: 249 DSSWEILIKERRTIHSQG-----------------------EVLSAGTDTTTTTVEWAMA 308
            + W  ++KE+R   S+                        E++ AG+DTTT+T+EWAM 
Sbjct: 250 INIWSEIVKEKRQAISRHSSDFLDVMIDSGFSDLQINILVTELMGAGSDTTTSTIEWAMT 309

Query: 309 ELLKNRDIMNRVREE-TKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAP 368
           ELL+N   M++++ E T K      I ES +SEL YL  CVKETLRLH P PFL+PR AP
Sbjct: 310 ELLRNNGAMHKLQAELTSKFGENDTITESNISELPYLAACVKETLRLHSPTPFLIPRRAP 369

Query: 369 EACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFLPFGG 428
           E C++M Y++PK++ +FVN + IGRD + WED  SFKPERF     D +G D+ F+PFG 
Sbjct: 370 ETCKIMNYTIPKNSKLFVNAYAIGRDSNTWEDALSFKPERFLDSKVDFRGQDFEFIPFGA 429

Query: 429 GRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPL 457
           GRRICPGL  A  +V LILA+LIH+F+W LPNDED   LD+  + GVTLQKE+PL
Sbjct: 430 GRRICPGLGFAIQEVHLILASLIHHFKWSLPNDEDPMQLDMEEKFGVTLQKEKPL 482

BLAST of CmoCh15G014850.1 vs. TAIR10
Match: AT2G45570.1 (AT2G45570.1 cytochrome P450, family 76, subfamily C, polypeptide 2)

HSP 1 Score: 319.3 bits (817), Expect = 3.9e-87
Identity = 192/495 (38.79%), Postives = 266/495 (53.74%), Query Frame = 1

Query: 6   FSFLLLLLPLIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGP 65
           F F+L    +IF       SS K  P PPGP   PI+GN+  +G+N H S    S  YGP
Sbjct: 13  FCFVLSFF-IIFFTTTRPRSSRKVVPSPPGPPRLPIIGNIHLVGRNPHHSFADLSKTYGP 72

Query: 66  LISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPL----HDNCSLLFSPE 125
           ++SLK G+   VV +SP AA  +L+T D++LS+R     TP   +    HD  S+++ P 
Sbjct: 73  IMSLKFGSLNTVVVTSPEAAREVLRTYDQILSSR-----TPTNSIRSINHDKVSVVWLPP 132

Query: 126 CGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQ--SKQGSVVEIKELVFITV 185
              RW+ LR +   +LF+ + IE+  +LR  K+ E+V F+   S++   V+I    FIT 
Sbjct: 133 SSSRWRLLRKLSATQLFSPQRIEATKTLRENKVKELVSFMSESSEREEAVDISRATFITA 192

Query: 186 FNTLSNFIFSRDLVGF------------------VGDGFNGIKGPFKNMIDLG-----LI 245
            N +SN +FS DL  +                  VG+       PF   +DL      L 
Sbjct: 193 LNIISNILFSVDLGNYDSNKSGVFQDTVIGVMEAVGNPDAANFFPFLGFLDLQGNRKTLK 252

Query: 246 PNLADFYPLLRCFDLQGLRKKAAIYKNQVD--------------SSWEILIKERRTIHSQ 305
                 + + R F    L +K+    N  D                 E  +     +H  
Sbjct: 253 ACSERLFKVFRGFIDAKLAEKSLRDTNSKDVRERDFVDVLLDLTEGDEAELNTNDIVHLL 312

Query: 306 GEVLSAGTDTTTTTVEWAMAELLKNRDIMNRVREETKKAING-GAIDESKLSELHYLWKC 365
            ++  AGTDT ++TVEWAMAELL+N + M + + E    I   G ++ES +S L YL   
Sbjct: 313 LDLFGAGTDTNSSTVEWAMAELLRNPETMVKAQAEIDCVIGQKGVVEESDISALPYLQAV 372

Query: 366 VKETLRLHPPAPFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPER 425
           VKET RLHP AP L+PR A    E++G+ VPK T +FVNVW IGRDP++WE+   FKPER
Sbjct: 373 VKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVFVNVWAIGRDPNVWENSSRFKPER 432

Query: 426 FDGCDADLKGHDYRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLD 457
           F G D DL+G DY   PFG GRRICPGLP+A   VPL+LA+L+++F+W LPN   +  LD
Sbjct: 433 FLGKDIDLRGRDYELTPFGAGRRICPGLPLAVKTVPLMLASLLYSFDWKLPNGVGSEDLD 492

BLAST of CmoCh15G014850.1 vs. TAIR10
Match: AT2G45560.1 (AT2G45560.1 cytochrome P450, family 76, subfamily C, polypeptide 1)

HSP 1 Score: 314.7 bits (805), Expect = 9.5e-86
Identity = 188/509 (36.94%), Postives = 278/509 (54.62%), Query Frame = 1

Query: 1   MEIASFSFLLLLLP------LIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHI 60
           M+I S   LLLL        LIF   R    S     LPPGP   PI+GN+  +G++ H 
Sbjct: 1   MDIISGQALLLLFCFILSCFLIFTTTRSGRISRGATALPPGPPRLPIIGNIHLVGKHPHR 60

Query: 61  SMTQFSHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPL---- 120
           S  + S  YGP++SLKLG+   VV +SP AA  +L+T D++LSAR     +P   +    
Sbjct: 61  SFAELSKTYGPVMSLKLGSLNTVVIASPEAAREVLRTHDQILSAR-----SPTNAVRSIN 120

Query: 121 HDNCSLLFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFL--QSKQGSV 180
           H + SL++ P    RW+ LR +   +L + + IE+  +LR  K+ E+V F+   S +   
Sbjct: 121 HQDASLVWLPSSSARWRLLRRLSVTQLLSPQRIEATKALRMNKVKELVSFISESSDREES 180

Query: 181 VEIKELVFITVFNTLSNFIFSRDLVGF-VGDGFNGIKGPFKNMIDLGLIPNLADFYPLLR 240
           V+I  + FIT  N +SN +FS DL  +      NG++    +++D    P+ A+++P LR
Sbjct: 181 VDISRVAFITTLNIISNILFSVDLGSYNAKASINGVQDTVISVMDAAGTPDAANYFPFLR 240

Query: 241 CFDLQG--------LRKKAAIYKNQVDSSWEILIKERRTIHSQGEV-------------- 300
             DLQG          +   +++  +D+     I E+ + ++  +V              
Sbjct: 241 FLDLQGNVKTFKVCTERLVRVFRGFIDAK----IAEKSSQNNPKDVSKNDFVDNLLDYKG 300

Query: 301 -----------------LSAGTDTTTTTVEWAMAELLKNRDIMNRVREETKKAI-NGGAI 360
                             +AGTDT+++T+EWAM ELLKN   M + + E    I   G +
Sbjct: 301 DESELSISDIEHLLLDMFTAGTDTSSSTLEWAMTELLKNPKTMAKAQAEIDCVIGQNGIV 360

Query: 361 DESKLSELHYLWKCVKETLRLHPPAPFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRD 420
           +ES +S+L YL   VKET RLH P P L+PR A    E++G+ V K T + VNVW IGRD
Sbjct: 361 EESDISKLPYLQAVVKETFRLHTPVPLLIPRKAESDAEILGFMVLKDTQVLVNVWAIGRD 420

Query: 421 PSIWEDPQSFKPERFDGCDADLKGHDYRFLPFGGGRRICPGLPMAAVQVPLILATLIHNF 457
           PS+W++P  F+PERF G D D++G DY   PFG GRRICPG+P+A   V L+LA+L+++F
Sbjct: 421 PSVWDNPSQFEPERFLGKDMDVRGRDYELTPFGAGRRICPGMPLAMKTVSLMLASLLYSF 480

BLAST of CmoCh15G014850.1 vs. TAIR10
Match: AT2G45550.1 (AT2G45550.1 cytochrome P450, family 76, subfamily C, polypeptide 4)

HSP 1 Score: 306.6 bits (784), Expect = 2.6e-83
Identity = 185/501 (36.93%), Postives = 266/501 (53.09%), Query Frame = 1

Query: 1   MEIASFSFLLLLLPLI---FII---LRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHI 60
           M+I S   L LL   I   F+I    R   SS +   LPPGP   PI+GN+ Q+G+N H 
Sbjct: 1   MDIISGQALFLLFCFISSCFLISTTARSRRSSGRAATLPPGPPRLPIIGNIHQVGKNPHS 60

Query: 61  SMTQFSHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNC 120
           S    + IYGP++SLK G    VV +SP AA  +L+T D++LS R           H+  
Sbjct: 61  SFADLAKIYGPIMSLKFGCLNSVVITSPEAAREVLRTHDQILSGRKSNDSIRCFG-HEEV 120

Query: 121 SLLFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQ--SKQGSVVEIK 180
           S+++ P    RW+ LR +    +F+ +  E+  +LR KK+ E+V F+   S++   V+I 
Sbjct: 121 SVIWLPPSSARWRMLRKLSVTLMFSPQRTEATKALRMKKVQELVSFMNESSERKEAVDIS 180

Query: 181 ELVFITVFNTLSNFIFSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYP-------- 240
              + TV N +SN +FS DL  +     N  +      ++    P+ A+++P        
Sbjct: 181 RASYTTVLNIISNILFSVDLGSYDSKKSNEFQDTVIGAMEAAGKPDAANYFPFMGFLDLQ 240

Query: 241 ------------LLRCF----DLQGLRKKAAIYKNQVDS------------SWEILIKER 300
                       L R F    D +   K    Y   V +              E  +   
Sbjct: 241 GNRKAMRGLTERLFRVFRGFMDAKIAEKSLGNYSKDVSNRDFLDSLLILNEGDEAELDNN 300

Query: 301 RTIHSQGEVLSAGTDTTTTTVEWAMAELLKNRDIMNRVREETKKAIN-GGAIDESKLSEL 360
              H   ++ +AGTDT+++T+EWAMAELL+N   M + + E  + +     + ES +S L
Sbjct: 301 DIEHLLLDMFTAGTDTSSSTLEWAMAELLRNPKTMVKAQAEMDRVLGQNSVVQESDISGL 360

Query: 361 HYLWKCVKETLRLHPPAPFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQ 420
            YL   VKET RLHP AP L+PR A    E++G+ VPK T + VNVW IGRDPS+WE+P 
Sbjct: 361 PYLQAVVKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVLVNVWAIGRDPSVWENPS 420

Query: 421 SFKPERFDGCDADLKGHDYRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDE 457
            F+PERF G D D+KG DY   PFGGGRRICPGLP+A   V L+LA+L+++F+W LPN  
Sbjct: 421 QFEPERFMGKDIDVKGRDYELTPFGGGRRICPGLPLAVKTVSLMLASLLYSFDWKLPNGV 480

BLAST of CmoCh15G014850.1 vs. TAIR10
Match: AT3G61040.1 (AT3G61040.1 cytochrome P450, family 76, subfamily C, polypeptide 7)

HSP 1 Score: 299.7 bits (766), Expect = 3.2e-81
Identity = 177/487 (36.34%), Postives = 271/487 (55.65%), Query Frame = 1

Query: 9   LLLLLPLIFIILRHISSSSKQPP----LPPGPKPWPILGNLLQIGQNAHISMTQFSHIYG 68
           ++L L  IF +     ++ K  P     PPGP    +L N+LQ  +  H S+   S IYG
Sbjct: 6   IVLSLLFIFFLFFFFYTTGKSCPGGAKNPPGPSKLSLLRNILQTVEKPHRSLADLSRIYG 65

Query: 69  PLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPL-HDNCSLLFSPECG 128
            ++S KLG    VV SSP  A  +LKT D +LS  Y     P R   H   SLL+ P   
Sbjct: 66  SVMSFKLGCLTTVVISSPETAKEVLKTHDHVLS--YRVSSDPVRAAGHHELSLLWIPPLA 125

Query: 129 DRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQS--KQGSVVEIKELVFITVFN 188
            RW+ LR I R +LF+ + +E+ +++R +K+ E++ F+    ++   V I    FIT  N
Sbjct: 126 -RWRFLRKITRNQLFSTQRLEATSAIRTRKVQELMNFVNKCCERREAVNISRASFITSLN 185

Query: 189 TLSNFIFSRDLVGFVGDG-FNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAI 248
            +SN +FS +L  F     F+  +     M+++   PNLADF+P L   DLQG RK+A +
Sbjct: 186 IISNALFSTNLANFDDSKTFHDFQNVVIRMMEISGKPNLADFFPFLGFLDLQGARKEARL 245

Query: 249 YKNQVDSSWEILIKERRTIHSQG-----------------------------EVLSAGTD 308
             +++   ++  I  +R+  S+                              ++  AG D
Sbjct: 246 LMHKLFRVFQGFIDTKRSSTSRNNNDMLDSLLDIAHKKESELDDNNIKHLLLDLFLAGVD 305

Query: 309 TTTTTVEWAMAELLKNRDIMNRVREETKKAIN-GGAIDESKLSELHYLWKCVKETLRLHP 368
           T+++ VEWAMAELL+N  ++ +V+EE ++ I   G + +  + +L YL   VKE+LRLHP
Sbjct: 306 TSSSAVEWAMAELLRNPKMIVKVQEEIRQVIGLKGTVQDLDIVKLPYLQAVVKESLRLHP 365

Query: 369 PAPFLLPRYAP-EACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADL 428
           PAPFL+PR +  +  ++  + +PK+T + VNVW IGRDP++W++P  F+PERF G   D+
Sbjct: 366 PAPFLVPRKSESDDVQIFEFLIPKNTQVLVNVWAIGRDPNVWKNPTQFEPERFLGRGIDV 425

Query: 429 KGHDYRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVT 457
           KG+ +  +PFG GRRICPG+P+A   + L+LA+L++ F+W   N      +D+N   G T
Sbjct: 426 KGNHFELIPFGAGRRICPGMPLAFRIMHLVLASLLYGFDWEYQNGVVPENVDMNEAFGAT 485

BLAST of CmoCh15G014850.1 vs. TAIR10
Match: AT1G33720.1 (AT1G33720.1 cytochrome P450, family 76, subfamily C, polypeptide 6)

HSP 1 Score: 292.7 bits (748), Expect = 3.9e-79
Identity = 184/496 (37.10%), Postives = 260/496 (52.42%), Query Frame = 1

Query: 6   FSFLLLLLPLIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGP 65
           F F+L  L L F   R   S  +    PPGP   PI+GN+  +G+N H S T  S  YGP
Sbjct: 13  FCFILSCL-LFFTTARSRRSPCQLSKSPPGPPRLPIIGNIHLVGKNPHHSFTDLSKTYGP 72

Query: 66  LISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDR 125
           ++SLKLG    VV +S  A   +LKT D++LS RYI + T     H+       P    R
Sbjct: 73  VMSLKLGCLNSVVIASRDAVREVLKTHDQILSGRYISEATKSNNHHEFSVGWIHPS-SSR 132

Query: 126 WKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQS--KQGSVVEIKELVFITVFNTL 185
           ++ LR +   +LF+ + I++  +LR KK+ E+V FL    ++   V+I  + F+T  N +
Sbjct: 133 FRMLRKLSATQLFSPQCIQATKALRMKKVQELVNFLSESCEREEAVDISHVSFVTALNII 192

Query: 186 SNFIFSRDLVGFVGDGFNGIKG------------------PFKNMIDLG----------- 245
           SN +FS +L  +     +  +                   PF   +DL            
Sbjct: 193 SNILFSVNLGSYDSKNSSAFQEMVIGYQESIGNPDLANFFPFMRFLDLQGNSKKMRESSG 252

Query: 246 -LIPNLADFYPLLRCFDLQGLRKKAAIYKNQVDSS--WEILIKERR----------TIHS 305
            L+    +FY      D + + K +   +  V S    ++LI  ++            H 
Sbjct: 253 RLLQVFREFY------DARIVEKSSRSVEKDVSSKDFLDVLIDLQQGDETEINIDEIEHL 312

Query: 306 QGEVLSAGTDTTTTTVEWAMAELLKNRDIMNRVREETKKAIN-GGAIDESKLSELHYLWK 365
             ++  AGTDT ++TVEWAMAELL N   M +V++E    I   G   ES +S+L YL  
Sbjct: 313 LLDMFVAGTDTNSSTVEWAMAELLGNPKTMTKVQDEINHVIGQNGDFQESDISKLPYLKA 372

Query: 366 CVKETLRLHPPAPFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPE 425
            VKET RLHP APFLL R A    E++G++V K + + VNVW IGRDP +WE+P  F+PE
Sbjct: 373 VVKETFRLHPAAPFLLQRKAETNVEILGFTVLKDSQVLVNVWAIGRDPLVWENPTHFEPE 432

Query: 426 RFDGCDADLKGHDYRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTL 457
           RF G + D+KG DY   PFG GRRICPGLP+A   V L+LA+L++ FEW LPN   +  L
Sbjct: 433 RFLGKEIDVKGTDYELTPFGAGRRICPGLPLAMKTVHLMLASLLYTFEWKLPNGVGSEDL 492

BLAST of CmoCh15G014850.1 vs. NCBI nr
Match: gi|659073029|ref|XP_008467223.1| (PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo])

HSP 1 Score: 708.4 bits (1827), Expect = 8.4e-201
Identity = 362/498 (72.69%), Postives = 404/498 (81.12%), Query Frame = 1

Query: 1   MEIASFSFL----LLLLPLIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISM 60
           M I SFS L    LLLLP IFIIL+HI S S + PLPPGP PWPILGNLLQIGQNAHIS+
Sbjct: 1   MLIPSFSILLPSLLLLLP-IFIILKHIKSPSSKLPLPPGPNPWPILGNLLQIGQNAHISI 60

Query: 61  TQFSHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSL 120
           TQF++IYGPLISLKLGAQRL+VASSPAAA A+LKTQDRLLSARYIFQMTPDR LHD  SL
Sbjct: 61  TQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSL 120

Query: 121 LFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVF 180
           +FSPECGD+WKNLRS+C+  LFTA+AIESQA LR KKM E+V+FL+SKQGSVVE+K+ VF
Sbjct: 121 VFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVF 180

Query: 181 ITVFNTLSNFIFSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRK 240
            +VFNTLSN IFS DL+ FVGDGF GIKGPFK M+DLGL PNLADFYPLLR FDLQGL+ 
Sbjct: 181 TSVFNTLSNLIFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKN 240

Query: 241 KAAIYKNQVDSSWEILIKERRTIHSQG--------------------------EVLSAGT 300
           KA IYKN+VDS W ILIKERR IH Q                           E+LSAGT
Sbjct: 241 KADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAGT 300

Query: 301 DTTTTTVEWAMAELLKNRDIMNRVREETKKAI-NGGAIDESKLSELHYLWKCVKETLRLH 360
           DTTTTTVEWAM+E+LK++DI+N+VREE KK       IDES +SEL YLW+CVKETLRLH
Sbjct: 301 DTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRLH 360

Query: 361 PPAPFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFD-GC--- 420
           PPAPFLLPR AP  CE+MGYS+PK +M+FVNVWGIGRDPS+WED Q+F P+RFD GC   
Sbjct: 361 PPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGND 420

Query: 421 DADLKGHDYRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGR 464
           + D KG+DYR+LPFGGGRRICPGLPMA VQVPLILATLIHNFEW LPNDED S LDLNGR
Sbjct: 421 NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGR 480

BLAST of CmoCh15G014850.1 vs. NCBI nr
Match: gi|778708326|ref|XP_004143707.2| (PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus])

HSP 1 Score: 544.7 bits (1402), Expect = 1.6e-151
Identity = 276/390 (70.77%), Postives = 307/390 (78.72%), Query Frame = 1

Query: 104 MTPDRPLHDNCSLLFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQS 163
           MTPDR L++  SL+FSPECGD WKNLRSIC+A LFTA+AIESQA LR KKM E+VEFL+S
Sbjct: 1   MTPDRALYNQHSLVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLES 60

Query: 164 KQGSVVEIKELVFITVFNTLSNFIFSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFY 223
           KQGSVV+IK+ VF +VFNTLSN IFS DL+ FVGDGFNGIKG FK M+DLGL PNLADFY
Sbjct: 61  KQGSVVKIKDFVFTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFY 120

Query: 224 PLLRCFDLQGLRKKAAIYKNQVDSSWEILIKERRTIHSQG-------------------- 283
           PLLR FDLQGLRKKAAIY N+VDS W ILIKERR IH Q                     
Sbjct: 121 PLLRRFDLQGLRKKAAIYMNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFSDQQ 180

Query: 284 ------EVLSAGTDTTTTTVEWAMAELLKNRDIMNRVREETKK-AINGGAIDESKLSELH 343
                 E+L A TDTTTTTVEWAM+E+LK++DI+N+ REE KK A    AIDES +S+L 
Sbjct: 181 IDALIIELLFAATDTTTTTVEWAMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQ 240

Query: 344 YLWKCVKETLRLHPPAPFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQS 403
           YLW CVKETLRLHPP PFLLPR AP  CE+MGYS+PK +MIFVNVWGIGRDPSIWED Q+
Sbjct: 241 YLWSCVKETLRLHPPVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQT 300

Query: 404 FKPERFD-GC--DADLKGHDYRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPN 463
           F PERFD GC  + D KG+DYR+LPFGGGRRICPGLPMA VQVPLILATL+HNFEW LPN
Sbjct: 301 FNPERFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPN 360

BLAST of CmoCh15G014850.1 vs. NCBI nr
Match: gi|743896671|ref|XP_011041604.1| (PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Populus euphratica])

HSP 1 Score: 506.1 bits (1302), Expect = 6.3e-140
Identity = 257/482 (53.32%), Postives = 335/482 (69.50%), Query Frame = 1

Query: 10  LLLLPLIFIILRHISS-SSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPLIS 69
           LLLLPL+F I++ ISS SSK   LPPGPKPWPI+GN+L  G+  HIS   F+  +GPLIS
Sbjct: 10  LLLLPLLFFIVKSISSPSSKNQSLPPGPKPWPIIGNILHFGKKPHISTANFAKTHGPLIS 69

Query: 70  LKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTP-DRPLHDNCSLLFSPECGDRWK 129
           LKLG Q L+V SS  AA  ILK+ DRLLSARY+F+    +  + D  S++++ +C D WK
Sbjct: 70  LKLGKQLLIVGSSKRAATEILKSHDRLLSARYVFKAALFENHVLDRISIVWATQCSDGWK 129

Query: 130 NLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 189
           +LR++CR +LF+A+AIESQA LR KKM EMVEF+  ++G VV I E+V   VFNT++N +
Sbjct: 130 SLRALCRNELFSAKAIESQAVLREKKMGEMVEFIGRRKGEVVGIGEVVLAIVFNTIANLL 189

Query: 190 FSRDLVGFVGDGF-NGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQVD 249
           FS DL+G   DG   G+K     M+ LG  PN++DFYP+L   D QGL++K A+  NQ+ 
Sbjct: 190 FSVDLIGLEDDGAATGLKSLMWRMMKLGATPNISDFYPILGGIDPQGLKRKMAVCVNQMF 249

Query: 250 SSWEILIKERRTIHSQG-------------------------EVLSAGTDTTTTTVEWAM 309
             W   IKERR  H                            E+LSAGTDTT TTVEWA+
Sbjct: 250 GIWGKYIKERREKHVHDGPRSDFLDVFLANGFEDLQINWLALELLSAGTDTTATTVEWAI 309

Query: 310 AELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAP 369
           AELLKN++++ +V EE K+  +  ++ ES +S+L YL  CV ETLRLHPP PFL+PR A 
Sbjct: 310 AELLKNKEVLKKVSEEIKRETDTNSLKESHVSQLPYLNACVNETLRLHPPVPFLIPRRAL 369

Query: 370 EACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFLPFGG 429
           E C++M Y++P+ + + VNVW +GRDPS+WEDP SFKPERF G D D KGHD+ FLPFG 
Sbjct: 370 ETCKVMDYTIPRDSEVIVNVWAVGRDPSLWEDPLSFKPERFLGSDLDFKGHDFEFLPFGA 429

Query: 430 GRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPLKLLSK 464
           GRRICPGLPMAA QV LI+ATL++ F+W LPN ED +TLD++ + G+TLQKE+PL L+ +
Sbjct: 430 GRRICPGLPMAAKQVHLIIATLLYYFDWSLPNGEDPATLDMSEKFGITLQKEQPLLLVPR 489

BLAST of CmoCh15G014850.1 vs. NCBI nr
Match: gi|255544572|ref|XP_002513347.1| (PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Ricinus communis])

HSP 1 Score: 504.2 bits (1297), Expect = 2.4e-139
Identity = 257/482 (53.32%), Postives = 336/482 (69.71%), Query Frame = 1

Query: 11  LLLPLIFIILRHISSS--SKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPLIS 70
           LLLPLI II+  I SS  SK+ PLPPGP+ WPILGN+LQ+G+N HISM  ++ ++GPLIS
Sbjct: 14  LLLPLILIIVMQILSSISSKRRPLPPGPRQWPILGNILQVGKNPHISMANYAKVHGPLIS 73

Query: 71  LKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTP-DRPLHDNCSLLFSPECGDRWK 130
           L+LG + +VVASSP AAA ILKT DRLLS RYI   TP +  + D  +L+++P C D+WK
Sbjct: 74  LRLGTRVVVVASSPTAAAEILKTHDRLLSGRYIPATTPYEDNVLDRIALVWNPSCSDQWK 133

Query: 131 NLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 190
            LR++CR++LF+A+AIESQA+LR KK+ EM++FL SKQG +V I E+VF T FNT+SN +
Sbjct: 134 FLRAMCRSELFSAKAIESQATLREKKLTEMLDFLTSKQGQIVNIGEVVFTTAFNTISNLL 193

Query: 191 FSRDLVGFVGDGFNG-IKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQVD 250
           FS+DL+ F   G  G +K     +++L   PN+ADFYP+L   D QG+++K      ++ 
Sbjct: 194 FSKDLLSFEDQGNAGELKTLISTLMELATCPNIADFYPVLTKLDPQGIKRKMKNCLERMF 253

Query: 251 SSWEILIKERRTIH--------------SQG-----------EVLSAGTDTTTTTVEWAM 310
             W+I IKERR  H              S G           E+ SAG DTTTTTVEWAM
Sbjct: 254 GVWDIYIKERRERHVKDARKTDFLDVFLSNGFDDHQINWLLLELFSAGADTTTTTVEWAM 313

Query: 311 AELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAP 370
           AELLK   ++ +VREE +  I    I ES + +L YL  CVKETLRLHPP PFL+PR AP
Sbjct: 314 AELLKEITVLEKVREELETEIGKDMIRESHIPQLKYLNACVKETLRLHPPVPFLIPRRAP 373

Query: 371 EACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFLPFGG 430
           EACE+M Y++PKH+ I VNVW IGRDPS WEDP S++PERF   + D KGH++ FLPFG 
Sbjct: 374 EACEVMNYTIPKHSQIIVNVWAIGRDPSAWEDPLSYRPERFLDSNLDFKGHNFEFLPFGS 433

Query: 431 GRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPLKLLSK 464
           GRRICPGLPM   Q+PLILA+L+H F+W L N +D + LD+N +  +TL+KE+ L ++ K
Sbjct: 434 GRRICPGLPMGTRQLPLILASLVHCFDWSLQNGDDPAMLDMNDKFSITLEKEQHLLVVPK 493

BLAST of CmoCh15G014850.1 vs. NCBI nr
Match: gi|255539531|ref|XP_002510830.1| (PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 [Ricinus communis])

HSP 1 Score: 497.7 bits (1280), Expect = 2.3e-137
Identity = 250/484 (51.65%), Postives = 333/484 (68.80%), Query Frame = 1

Query: 10  LLLLPLIFIILRHI----SSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGP 69
           LL LP++ +IL  I    SS  +  PLPPGPKPWPILGN+LQ+ +  HI +  F+ +YGP
Sbjct: 10  LLPLPILVVILTQIISFISSRRQHLPLPPGPKPWPILGNILQVDKKLHICIANFAKVYGP 69

Query: 70  LISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTP-DRPLHDNCSLLFSPECGD 129
           LISL+LG Q LVVAS+P++AA +LK  DRLLSAR+I +  P +  + +  +++++P C D
Sbjct: 70  LISLRLGNQVLVVASTPSSAAEVLKNHDRLLSARFILKAIPSESHILERVAIVWNPACND 129

Query: 130 RWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVFNTLS 189
            WK+LR++CR +LF+ +AIESQA LR KK+ EM+EFL +KQG  V + E+VF T+FNT+S
Sbjct: 130 HWKSLRALCRTELFSPKAIESQAILREKKLAEMLEFLITKQGQAVNVAEVVFGTIFNTIS 189

Query: 190 NFIFSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQ 249
           + +FS DL+GF      G+K    +M+++   PN+A+FYP+L   D QGL++K      +
Sbjct: 190 HLLFSTDLIGFENQ-IGGVKSLLWSMMEMATSPNIAEFYPILAPLDPQGLKRKMTKCLKE 249

Query: 250 VDSSWEILIKERRTIH----------------SQG-----------EVLSAGTDTTTTTV 309
           +   WEI IKERR  H                S G           E+LSAGTDTT+T+V
Sbjct: 250 MFGVWEIYIKERRRTHDHDHAAPKTDFLDIFLSNGFDDDQINWLVMELLSAGTDTTSTSV 309

Query: 310 EWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLP 369
           EWAMAELLKN++ M +VREE  + IN   I ES +S+L YL  CVKETLRLHPPAPFL+P
Sbjct: 310 EWAMAELLKNKEAMKKVREELDREINKNPIKESHVSQLPYLNACVKETLRLHPPAPFLIP 369

Query: 370 RYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFL 429
           R A E CE+M Y++PK + + VNVW IGRDPS+WEDP SFKPERF G   D+KGHD+  +
Sbjct: 370 RRATENCEVMNYTIPKDSQVLVNVWAIGRDPSVWEDPSSFKPERFLGSSLDVKGHDFELI 429

Query: 430 PFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPLK 462
           PFG GRRICPGLPMA  Q+ L+LA+LIH F+W LPN  D + +D+  + G+TLQ E PL 
Sbjct: 430 PFGSGRRICPGLPMATRQLSLVLASLIHCFDWSLPNGGDPAKVDMTEKFGITLQMEHPLL 489