CmoCh06G000510.1 (mRNA) Cucurbita moschata (Rifu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmoCh06G000510.1
Type	mRNA
Organism	Cucurbita moschata (Cucurbita moschata (Rifu))
Description	Membrane steroid-binding protein 2
Location	Cmo_Chr06 : 322488 .. 325413 (+)
Sequence length	1050

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACCATGGAGGGAGTAGGTAAAGGATTCATGGTTGGTGAAGTTGGGACTTTTATCTTCAAATCCAGTCCACCGGAATCTATTGCGTCGGGGAGAAAATTGCAGTCGCAAAGCTAGAGCGAAGAAATGGAGATCTTCTGGAGAGCGATGGAACAGATCAATTGGTACACCGGATTATCGCCCACCGCCTTCTTCACCATACTTGCCTCAATGATCTTCGTGTTTCAAATGGTTTCCTCCATGTTCGTTTCTCCAGAAGAGTTCAATAAACCCCCAACTGTTCCTGTTTCTTCATCTAACTCGGCCAATTCGAATTTGTTTGTTGACGATTCGGTCGCCGACGCCTCGCAATCAGTCCAAGTAGGGCGATTGACGGAGCAGCAGTTGAGGGCTTACGATGGCTCCGATCCGAATAAGCCACTTCTTATGGCTATAAAGGGTCAAATCTACGATGTATCTTCCGGCAGGTACTTTTTTCTTTTTCTTTTCCTTCATCATTCGTGGAGCGAATTCCGTTTGGTCTCTATTTAGAAATTTGCTCATAACTTGCATAGTTGCCTACTTTCATGAGTTGAAGCAATGAGTATGAAAATTCTTCTTAATCCTCGCTCTAGATGAAGAACTACTTCTGTTTATCTCATGACTGGAGTTCAAGAATTGCAGAATTTTAAACAAAACTGACCAACCGGTGATATTTATTGAGGATATACATTAGAATTTCTTGAGCACACTTCCCGTTGTAATCCCATTTCCACTACTTGTCAATTGTCATATTGTCTGTTTTACGTCAAAGTTCCACTGATCAGCTCAAACTGTTAAGATCTTTGGCCAAGATTTTCAAGGAGGAGAATATTTTTGCACAAAATGTGTTGGCGAGAGGATTGTCAAGCCTTCCATTCGGTTTCTTTGTGTGAGAAAAAGGGTGGTCGGTGTTGGATGAAAGTCCCACATTGACTAATTTAGGGAATGATCATGGATTTATACCATTGTGGAGAGTGTGTTCATCTAACAGTAAGGAGTAAAAGAATGACAGTTGGGCTGTGTGGAACTGGGGACTGAAGTTGCAGAAGGGAAGGGGAAGTTTTTTTTCCTCTCTCTGTGAAAGCGGCTAATAACAGAAATTTTGTAAGTCAACCAGTCTGTTGGGGAATATTAAACTCATGGTCGAGTTTAGGAAGCTTTAGGGTTGGAAAGCCTCTCCATATTTATGTAATACATGGCTGGATTTGTGATATCTCACATCAGTTGGAGAGGGGGAACGAAACATTCCTTATAAGTGTGTGGAAACCTCTCCCTAGTGGACGCGTTTTAAAACCTTGAGGGAAAACCTAGAAGGGAAAGCTCAAAGAGGACAATATTTACTAGCGTGGGCTTGGGCTGTTACAAATGGTATCAGAGCCAGACATCGGACGGTATGTCATTGAGGACGTTGGGCCATTAGAGGGGTGGATTGTGAGATCTCACGTCGGTTAGAGAGGGGAACGAAGCATTTCTTATAAGGTGTGGAAATCTCTCCCTAGTGGACGTGTTTTAAAATCTTAAGGGGAAGCCCATAAGGAAAAGCGGACAATATCTGCTAGCGGTGAGCTTGGGTTGTTACAGGATTGCTCAAACCAATGAACTATGAAGGATGTAGTATACGAAAAGAGTTTCCTAATCTTGAGTTTATGTACACTTAGTGTAATGACCAAAATAGGGTATGAAATTAGTAAATTCGTTGCTTGAAATAAGCAAACAGGCAGACCAAGTACAGACTTCTTAACTTTGTAGATTTTGTTTCGGGACGGGGTAAATGCAGTGATGTAAGGCAAGGGTAGTTGTTAATAGAGACTAGAAGGGACATGCAGCCGTTTGATGGTTCTATTTTGTCCCATGTTTCAAATACGAATATTATTGGAATTAAGACATCGGCAGTATAGAAACTATTAAGTTCTCTTCCAAACTACTACTGATATTGTAGGACAAGCACAGAACTTTGGGTTTCTCAAAGTTCATACGTTCCTTGAGAAATTAGAACTTTTGGCTTGTGTGAATTTATAAATTCTGAAAATAGAATTCATTACGTACATGTCTTAACTCGTTGAGCTATTACAATTACAATTGTATTGCACTTCAGCCATAATTTTTACACGACCACTTGACCCATCCAATTGAGTATCATATTGCACCCACCTAAAGTTCTATAAATCAAGTGTTATGTTTGCATTTCATCAACTGTTCTTAAGGATAGGGACTTGACAAACCTAATTCTGGACAAAATTGAAACTTCTTAAGAGTTGAAAGACTAAATTGAAAGATGTGTGATTGCATAGGATGACACTGTGTTTTTGGTTGGCTTTTCAGGACATTTTATGGTCCTGGTTCGCCTTACTCCATGTTCGTCGGGAAGGATGCTAGCAGAGCCTTGGCCCTCTTGTCTTTTAAACCTGAAGACATTAATGGTAATCTCGAAGACCTGAATGAAGAAGAGCTTGTAATCTTGCAGGATTGGGAATACAAGTTCATGGAAAAGTATGTGAAGGTTGGGGAGCTCGTTTCAGAGGAGGCATTGAATGAGCGTTCTGAAAATGGACACCAAAGTACCGATACCAATCGAGAAGAAGAACCCAACAAAGATCAATAAGAAGGCGTTTATTGCTTTCACCTTTCATCAGGCTGATCACTGGAAGTTCTTGACTTGAATGTGCCTGTCCTTTGCCCTTCCTCCGAATATATATAAGATATTGTGACTAGAAGTGATCAATCCGATTGAAGTTAGATATCCTGCAACATCTTGGCTAGATTTTTGGTTTTGTATATTACTTCACACGAAAATTTTTAATACATATTATTACCTACCGTTTGATGTGTGATATATTATTTTATTTTATAGGTCAAAACATTTAAAAAAGGTTGGCCGTCACAAACAATGAACCAAAGTTTACGTTAC

mRNA sequence

ACCATGGAGGGAGTAGGTAAAGGATTCATGGTTGGTGAAGTTGGGACTTTTATCTTCAAATCCAGTCCACCGGAATCTATTGCGTCGGGGAGAAAATTGCAGTCGCAAAGCTAGAGCGAAGAAATGGAGATCTTCTGGAGAGCGATGGAACAGATCAATTGGTACACCGGATTATCGCCCACCGCCTTCTTCACCATACTTGCCTCAATGATCTTCGTGTTTCAAATGGTTTCCTCCATGTTCGTTTCTCCAGAAGAGTTCAATAAACCCCCAACTGTTCCTGTTTCTTCATCTAACTCGGCCAATTCGAATTTGTTTGTTGACGATTCGGTCGCCGACGCCTCGCAATCAGTCCAAGTAGGGCGATTGACGGAGCAGCAGTTGAGGGCTTACGATGGCTCCGATCCGAATAAGCCACTTCTTATGGCTATAAAGGGTCAAATCTACGATGTATCTTCCGGCAGGACATTTTATGGTCCTGGTTCGCCTTACTCCATGTTCGTCGGGAAGGATGCTAGCAGAGCCTTGGCCCTCTTGTCTTTTAAACCTGAAGACATTAATGGTAATCTCGAAGACCTGAATGAAGAAGAGCTTGTAATCTTGCAGGATTGGGAATACAAGTTCATGGAAAAGTATGTGAAGGTTGGGGAGCTCGTTTCAGAGGAGGCATTGAATGAGCGTTCTGAAAATGGACACCAAAGTACCGATACCAATCGAGAAGAAGAACCCAACAAAGATCAATAAGAAGGCGTTTATTGCTTTCACCTTTCATCAGGCTGATCACTGGAAGTTCTTGACTTGAATGTGCCTGTCCTTTGCCCTTCCTCCGAATATATATAAGATATTGTGACTAGAAGTGATCAATCCGATTGAAGTTAGATATCCTGCAACATCTTGGCTAGATTTTTGGTTTTGTATATTACTTCACACGAAAATTTTTAATACATATTATTACCTACCGTTTGATGTGTGATATATTATTTTATTTTATAGGTCAAAACATTTAAAAAAGGTTGGCCGTCACAAACAATGAACCAAAGTTTACGTTAC

Coding sequence (CDS)

ATGGAGATCTTCTGGAGAGCGATGGAACAGATCAATTGGTACACCGGATTATCGCCCACCGCCTTCTTCACCATACTTGCCTCAATGATCTTCGTGTTTCAAATGGTTTCCTCCATGTTCGTTTCTCCAGAAGAGTTCAATAAACCCCCAACTGTTCCTGTTTCTTCATCTAACTCGGCCAATTCGAATTTGTTTGTTGACGATTCGGTCGCCGACGCCTCGCAATCAGTCCAAGTAGGGCGATTGACGGAGCAGCAGTTGAGGGCTTACGATGGCTCCGATCCGAATAAGCCACTTCTTATGGCTATAAAGGGTCAAATCTACGATGTATCTTCCGGCAGGACATTTTATGGTCCTGGTTCGCCTTACTCCATGTTCGTCGGGAAGGATGCTAGCAGAGCCTTGGCCCTCTTGTCTTTTAAACCTGAAGACATTAATGGTAATCTCGAAGACCTGAATGAAGAAGAGCTTGTAATCTTGCAGGATTGGGAATACAAGTTCATGGAAAAGTATGTGAAGGTTGGGGAGCTCGTTTCAGAGGAGGCATTGAATGAGCGTTCTGAAAATGGACACCAAAGTACCGATACCAATCGAGAAGAAGAACCCAACAAAGATCAATAA

Homology

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BLAST of CmoCh06G000510.1 vs. Swiss-Prot
Match: MSBP2_ARATH (Membrane steroid-binding protein 2 OS=Arabidopsis thaliana GN=MSBP2 PE=1 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 3.9e-41
Identity = 94/196 (47.96%), Postives = 123/196 (62.76%), Query Frame = 1


Query: 5   WRAM-EQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSANSN 64
           W  + E I  YTGLSP AFFT+LA    V+Q+VS  FVSPE  ++P ++ V   +     
Sbjct: 6   WETLKETITAYTGLSPAAFFTVLALAFAVYQVVSGFFVSPE-VHRPRSLEVQPQSEP--- 65

Query: 65  LFVDDSVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPGSPY 124
                        VQ+G +TE++L+ YDGSD  KPLLMAIKGQIYDVS  R FYGPG PY
Sbjct: 66  ---------LPPPVQLGEITEEELKLYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGGPY 125

Query: 125 SMFVGKDASRALALLSFKPEDINGNLEDLNEEELVILQDWEYKFMEKYVKVGELVSEEAL 184
           ++F GKDASRALA +SF+ +D+ G++  L   EL  LQDWEYKFM KYVKVG +  ++  
Sbjct: 126 ALFAGKDASRALAKMSFEDQDLTGDISGLGAFELEALQDWEYKFMSKYVKVGTIQKKDGE 185

Query: 185 NERSENGHQSTDTNRE 200
            + S    ++   + E
Sbjct: 186 GKESSEPSEAKTASAE 188

[Close]

BLAST of CmoCh06G000510.1 vs. Swiss-Prot
Match: MSBP1_ARATH (Membrane steroid-binding protein 1 OS=Arabidopsis thaliana GN=MSBP1 PE=1 SV=2)

HSP 1 Score: 161.4 bits (407), Expect = 1.1e-38
Identity = 87/175 (49.71%), Postives = 111/175 (63.43%), Query Frame = 1


Query: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60
           +E++    E I+ YTGLSP  FFT LA    ++Q++S  F SP +        V+    A
Sbjct: 3   LELWQTLKEAIHAYTGLSPVVFFTALALAFAIYQVISGWFASPFD-------DVNRHQRA 62

Query: 61  NSNLFVDDSVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG 120
            S     +      Q VQVG +TE++L+ YDGSDP KPLLMAIK QIYDV+  R FYGPG
Sbjct: 63  RS--LAQEEEPPIPQPVQVGEITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPG 122

Query: 121 SPYSMFVGKDASRALALLSFKPEDINGNLEDLNEEELVILQDWEYKFMEKYVKVG 176
            PY++F GKDASRALA +SF+ +D+  ++  L   EL  LQDWEYKFM KY KVG
Sbjct: 123 GPYALFAGKDASRALAKMSFEEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVG 168

[Close]

BLAST of CmoCh06G000510.1 vs. Swiss-Prot
Match: SBP3_ARATH (Probable steroid-binding protein 3 OS=Arabidopsis thaliana GN=MP3 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 4.8e-23
Identity = 51/97 (52.58%), Postives = 71/97 (73.20%), Query Frame = 1


Query: 83  TEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPGSPYSMFVGKDASRALALLSFKP 142
           T +QL  Y+G+D +KP+ +AIKG+++DV++G++FYG G  YSMF GKDASRAL  +S   
Sbjct: 4   TAEQLSQYNGTDESKPIYVAIKGRVFDVTTGKSFYGSGGDYSMFAGKDASRALGKMSKNE 63

Query: 143 EDINGNLEDLNEEELVILQDWEYKFMEKYVKVGELVS 180
           ED++ +LE L E+E+  L DWE KF  KY  VG +VS
Sbjct: 64  EDVSPSLEGLTEKEINTLNDWETKFEAKYPVVGRVVS 100

[Close]

BLAST of CmoCh06G000510.1 vs. Swiss-Prot
Match: NEUFC_DANRE (Neuferricin OS=Danio rerio GN=cyb5d2 PE=3 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.7e-15
Identity = 50/154 (32.47%), Postives = 80/154 (51.95%), Query Frame = 1


Query: 25  ILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSANSNLFVDDSVADASQSVQVGRLTE 84
           +L  ++ +  MV +++  PE      T P+  +       ++   V++ S S     LT+
Sbjct: 1   MLKYLVALISMVLAVWTVPEWL----TFPIYGNVVTVLESWLRQRVSEVSASSPGLLLTK 60

Query: 85  QQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPGSPYSMFVGKDASRALALLSFKPED 144
           +QL  Y+G   +K L +AI GQ++DV  GR  YGPG  Y  F GKDASRA     F    
Sbjct: 61  EQLSLYNGGKNSKGLYLAILGQVFDVEKGRKHYGPGGGYHFFTGKDASRAFITGDFTEAG 120

Query: 145 INGNLEDLNEEELVILQDWEYKFMEKYVKVGELV 179
           ++ ++ D +E ++V L DW   +   Y  VG+L+
Sbjct: 121 LSNDVSDFSESQIVALYDWLSFYQRDYTPVGKLI 150

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BLAST of CmoCh06G000510.1 vs. Swiss-Prot
Match: DAP1_SCHPO (Cytochrome P450 regulator dap1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=dap1 PE=1 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.1e-14
Identity = 47/104 (45.19%), Postives = 60/104 (57.69%), Query Frame = 1


Query: 83  TEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPGSPYSMFVGKDASRALALLSFKP 142
           T  +L+ Y+GS  N  + +AIKG +Y+V+ G  FYGP  PYS F G DASR LA  SF  
Sbjct: 44  TPAELKEYNGSK-NSLVFLAIKGTVYNVTMGSKFYGPQGPYSAFAGHDASRGLAKNSFDD 103

Query: 143 EDIN-------GNLEDLNEEELVILQDWEYKFMEKYVKVGELVS 180
           E I         +  DLN+EE   L DW+  F +KY  VG L+S
Sbjct: 104 EFIPDSDAEELDDCSDLNDEERQALNDWKAFFDQKYQAVGRLIS 146

[Close]

BLAST of CmoCh06G000510.1 vs. TrEMBL
Match: E5GBX8_CUCME (Steroid binding protein OS=Cucumis melo subsp. melo PE=3 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 1.4e-90
Identity = 178/207 (85.99%), Postives = 192/207 (92.75%), Query Frame = 1


Query: 1   MEIFWR-AMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNS 60
           ME +WR AMEQI WYTGLSPTAFFTILA+MIFVFQMVSSMFVSPEEFNKPPTVP+SSSNS
Sbjct: 1   MEFYWRSAMEQITWYTGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNS 60

Query: 61  ANSNLFVDDSVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGP 120
           +NSNLFV+DS ADASQ+V VGRLTEQQLRAY+GSDPNKPLLMAIKGQIYDVSSGR FYGP
Sbjct: 61  SNSNLFVNDSAADASQAVLVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRIFYGP 120

Query: 121 GSPYSMFVGKDASRALALLSFKPEDINGNLEDLNEEELVILQDWEYKFMEKYVKVGELVS 180
           GSPYSMFVGKDASRALALLSFKPEDINGN+E L+EEELVILQDWEYKFMEKYVKVGELV 
Sbjct: 121 GSPYSMFVGKDASRALALLSFKPEDINGNIEGLSEEELVILQDWEYKFMEKYVKVGELVP 180

Query: 181 EEALNERSENGHQSTDTNREEEPNKDQ 207
           E  LNE SENGHQ+++T + E  +KDQ
Sbjct: 181 EGELNEHSENGHQNSETTQVEH-DKDQ 206

[Close]

BLAST of CmoCh06G000510.1 vs. TrEMBL
Match: A0A0A0LC44_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G815470 PE=3 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 2.4e-90
Identity = 178/207 (85.99%), Postives = 192/207 (92.75%), Query Frame = 1


Query: 1   MEIFWR-AMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNS 60
           ME +W+ AMEQI WYTGLSPTAFFTILA+MIFVFQMVSSMFVSPEEFNKPPTV +SSSNS
Sbjct: 1   MEFYWKSAMEQITWYTGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVAISSSNS 60

Query: 61  ANSNLFVDDSVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGP 120
           +NSNLFV+DS ADAS +VQVGRLTEQQLRAY+GSDPNKPLLMAIKGQIYDVSSGR FYGP
Sbjct: 61  SNSNLFVNDSGADASLAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGP 120

Query: 121 GSPYSMFVGKDASRALALLSFKPEDINGNLEDLNEEELVILQDWEYKFMEKYVKVGELVS 180
           GSPYSMFVGKDASRALALLSFKPEDINGN+E LNEEELVILQDWEYKFMEKYVKVGELV 
Sbjct: 121 GSPYSMFVGKDASRALALLSFKPEDINGNIEGLNEEELVILQDWEYKFMEKYVKVGELVP 180

Query: 181 EEALNERSENGHQSTDTNREEEPNKDQ 207
           E  LNE SENGHQ+++T +EE  +KDQ
Sbjct: 181 EGELNEHSENGHQNSETTQEEH-DKDQ 206

[Close]

BLAST of CmoCh06G000510.1 vs. TrEMBL
Match: W9S7V8_9ROSA (Membrane steroid-binding protein 2 OS=Morus notabilis GN=L484_027995 PE=3 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 7.1e-58
Identity = 119/182 (65.38%), Postives = 144/182 (79.12%), Query Frame = 1


Query: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60
           M I+  A+E+I WYTGLSP AFFTI A M+ V++ VSSMFVSPE+FNKPP        S 
Sbjct: 1   MGIYSAAVEEITWYTGLSPAAFFTIAALMVVVYKTVSSMFVSPEDFNKPPVAMSIEKISQ 60

Query: 61  NSNLFVDDSVADA--SQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYG 120
           +++ F+++ +A    SQ +Q+G +TEQQLRAY+GSDPNKPLL++IKGQIYDVSS R FYG
Sbjct: 61  SNSDFLNNVMATTTNSQPLQLGDMTEQQLRAYNGSDPNKPLLISIKGQIYDVSSSRMFYG 120

Query: 121 PGSPYSMFVGKDASRALALLSFKPEDINGNLEDLNEEELVILQDWEYKFMEKYVKVGELV 180
           PG  YSMF GK+ASRALALLSFKP+DINGNLEDL  EEL +L+DWE KF EKY KVG+LV
Sbjct: 121 PGGAYSMFTGKEASRALALLSFKPQDINGNLEDLGPEELQVLEDWEDKFTEKYAKVGQLV 180

[Close]

BLAST of CmoCh06G000510.1 vs. TrEMBL
Match: A0A0R0EIY9_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_19G064800 PE=3 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 9.3e-58
Identity = 122/201 (60.70%), Postives = 151/201 (75.12%), Query Frame = 1


Query: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60
           M ++   ME+I++YTGLSP AFFTILA M+ V++ VS MFVSPE++NKPP V    S  A
Sbjct: 1   MGLYTSVMEEISFYTGLSPAAFFTILAMMVVVYRTVSGMFVSPEDYNKPPVV----SARA 60

Query: 61  NSNLFVDDSVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG 120
           NS L   D      + VQ+G +T+++LRAYDGSDP+KPLLMAIKGQIYDVS+GR FYGPG
Sbjct: 61  NSRL---DESEPPREPVQLGEITDRELRAYDGSDPSKPLLMAIKGQIYDVSNGRNFYGPG 120

Query: 121 SPYSMFVGKDASRALALLSFKPEDINGNLEDLNEEELVILQDWEYKFMEKYVKVGELVSE 180
            PY+MF GK+ SRALALLSFKP+DINGNLE L EEEL IL+DWE+KF+EKY KVG+L++E
Sbjct: 121 GPYAMFAGKECSRALALLSFKPDDINGNLEGLGEEELTILEDWEFKFIEKYPKVGQLIAE 180

Query: 181 EAL--NERSENGHQSTDTNRE 200
           +    NE  E    + D   E
Sbjct: 181 QRTRQNEFKEQTQDNLDNPNE 194

[Close]

BLAST of CmoCh06G000510.1 vs. TrEMBL
Match: A0A0B2SJ61_GLYSO (Membrane steroid-binding protein 2 OS=Glycine soja GN=glysoja_049787 PE=3 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 9.3e-58
Identity = 122/201 (60.70%), Postives = 151/201 (75.12%), Query Frame = 1


Query: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60
           M ++   ME+I++YTGLSP AFFTILA M+ V++ VS MFVSPE++NKPP V    S  A
Sbjct: 1   MGLYTSVMEEISFYTGLSPAAFFTILAMMVVVYRTVSGMFVSPEDYNKPPVV----SARA 60

Query: 61  NSNLFVDDSVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG 120
           NS L   D      + VQ+G +T+++LRAYDGSDP+KPLLMAIKGQIYDVS+GR FYGPG
Sbjct: 61  NSRL---DESEPPREPVQLGEITDRELRAYDGSDPSKPLLMAIKGQIYDVSNGRNFYGPG 120

Query: 121 SPYSMFVGKDASRALALLSFKPEDINGNLEDLNEEELVILQDWEYKFMEKYVKVGELVSE 180
            PY+MF GK+ SRALALLSFKP+DINGNLE L EEEL IL+DWE+KF+EKY KVG+L++E
Sbjct: 121 GPYAMFAGKECSRALALLSFKPDDINGNLEGLGEEELTILEDWEFKFIEKYPKVGQLIAE 180

Query: 181 EAL--NERSENGHQSTDTNRE 200
           +    NE  E    + D   E
Sbjct: 181 QRTRQNEFKEQTQDNLDNPNE 194

[Close]

BLAST of CmoCh06G000510.1 vs. TAIR10
Match: AT3G48890.1 (AT3G48890.1 membrane-associated progesterone binding protein 3)

HSP 1 Score: 169.5 bits (428), Expect = 2.2e-42
Identity = 94/196 (47.96%), Postives = 123/196 (62.76%), Query Frame = 1


Query: 5   WRAM-EQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSANSN 64
           W  + E I  YTGLSP AFFT+LA    V+Q+VS  FVSPE  ++P ++ V   +     
Sbjct: 6   WETLKETITAYTGLSPAAFFTVLALAFAVYQVVSGFFVSPE-VHRPRSLEVQPQSEP--- 65

Query: 65  LFVDDSVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPGSPY 124
                        VQ+G +TE++L+ YDGSD  KPLLMAIKGQIYDVS  R FYGPG PY
Sbjct: 66  ---------LPPPVQLGEITEEELKLYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGGPY 125

Query: 125 SMFVGKDASRALALLSFKPEDINGNLEDLNEEELVILQDWEYKFMEKYVKVGELVSEEAL 184
           ++F GKDASRALA +SF+ +D+ G++  L   EL  LQDWEYKFM KYVKVG +  ++  
Sbjct: 126 ALFAGKDASRALAKMSFEDQDLTGDISGLGAFELEALQDWEYKFMSKYVKVGTIQKKDGE 185

Query: 185 NERSENGHQSTDTNRE 200
            + S    ++   + E
Sbjct: 186 GKESSEPSEAKTASAE 188

[Close]

BLAST of CmoCh06G000510.1 vs. TAIR10
Match: AT5G52240.1 (AT5G52240.1 membrane steroid binding protein 1)

HSP 1 Score: 161.4 bits (407), Expect = 6.0e-40
Identity = 87/175 (49.71%), Postives = 111/175 (63.43%), Query Frame = 1


Query: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60
           +E++    E I+ YTGLSP  FFT LA    ++Q++S  F SP +        V+    A
Sbjct: 3   LELWQTLKEAIHAYTGLSPVVFFTALALAFAIYQVISGWFASPFD-------DVNRHQRA 62

Query: 61  NSNLFVDDSVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG 120
            S     +      Q VQVG +TE++L+ YDGSDP KPLLMAIK QIYDV+  R FYGPG
Sbjct: 63  RS--LAQEEEPPIPQPVQVGEITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPG 122

Query: 121 SPYSMFVGKDASRALALLSFKPEDINGNLEDLNEEELVILQDWEYKFMEKYVKVG 176
            PY++F GKDASRALA +SF+ +D+  ++  L   EL  LQDWEYKFM KY KVG
Sbjct: 123 GPYALFAGKDASRALAKMSFEEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVG 168

[Close]

BLAST of CmoCh06G000510.1 vs. TAIR10
Match: AT2G24940.1 (AT2G24940.1 membrane-associated progesterone binding protein 2)

HSP 1 Score: 109.4 bits (272), Expect = 2.7e-24
Identity = 51/97 (52.58%), Postives = 71/97 (73.20%), Query Frame = 1


Query: 83  TEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPGSPYSMFVGKDASRALALLSFKP 142
           T +QL  Y+G+D +KP+ +AIKG+++DV++G++FYG G  YSMF GKDASRAL  +S   
Sbjct: 4   TAEQLSQYNGTDESKPIYVAIKGRVFDVTTGKSFYGSGGDYSMFAGKDASRALGKMSKNE 63

Query: 143 EDINGNLEDLNEEELVILQDWEYKFMEKYVKVGELVS 180
           ED++ +LE L E+E+  L DWE KF  KY  VG +VS
Sbjct: 64  EDVSPSLEGLTEKEINTLNDWETKFEAKYPVVGRVVS 100

[Close]

BLAST of CmoCh06G000510.1 vs. TAIR10
Match: AT4G14965.1 (AT4G14965.1 membrane-associated progesterone binding protein 4)

HSP 1 Score: 74.3 bits (181), Expect = 9.6e-14
Identity = 36/116 (31.03%), Postives = 61/116 (52.59%), Query Frame = 1


Query: 63  NLFVDDSVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPGSP 122
           +L+   S        Q    + ++L  Y+G+D   P+L+ I G ++DV+ G+  YG G  
Sbjct: 23  SLYFRSSFKSPQHQYQKRLFSAEELALYNGTDETLPILLGILGSVFDVTKGKFHYGSGGG 82

Query: 123 YSMFVGKDASRALALLSFKPEDINGNLEDLNEEELVILQDWEYKFMEKYVKVGELV 179
           Y+ F G+DASRA    +F  + +  +L+ L+  E+  + DW   +   Y  VG+LV
Sbjct: 83  YNHFAGRDASRAFVSGNFTGDGLTDSLQGLSSSEVKSIVDWRGFYSRTYTPVGKLV 138

[Close]

BLAST of CmoCh06G000510.1 vs. NCBI nr
Match: gi|659084981|ref|XP_008443179.1| (PREDICTED: membrane steroid-binding protein 2-like [Cucumis melo])

HSP 1 Score: 340.5 bits (872), Expect = 2.0e-90
Identity = 178/207 (85.99%), Postives = 192/207 (92.75%), Query Frame = 1


Query: 1   MEIFWR-AMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNS 60
           ME +WR AMEQI WYTGLSPTAFFTILA+MIFVFQMVSSMFVSPEEFNKPPTVP+SSSNS
Sbjct: 1   MEFYWRSAMEQITWYTGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNS 60

Query: 61  ANSNLFVDDSVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGP 120
           +NSNLFV+DS ADASQ+V VGRLTEQQLRAY+GSDPNKPLLMAIKGQIYDVSSGR FYGP
Sbjct: 61  SNSNLFVNDSAADASQAVLVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRIFYGP 120

Query: 121 GSPYSMFVGKDASRALALLSFKPEDINGNLEDLNEEELVILQDWEYKFMEKYVKVGELVS 180
           GSPYSMFVGKDASRALALLSFKPEDINGN+E L+EEELVILQDWEYKFMEKYVKVGELV 
Sbjct: 121 GSPYSMFVGKDASRALALLSFKPEDINGNIEGLSEEELVILQDWEYKFMEKYVKVGELVP 180

Query: 181 EEALNERSENGHQSTDTNREEEPNKDQ 207
           E  LNE SENGHQ+++T + E  +KDQ
Sbjct: 181 EGELNEHSENGHQNSETTQVEH-DKDQ 206

[Close]

BLAST of CmoCh06G000510.1 vs. NCBI nr
Match: gi|449437656|ref|XP_004136607.1| (PREDICTED: membrane steroid-binding protein 2-like [Cucumis sativus])

HSP 1 Score: 339.7 bits (870), Expect = 3.5e-90
Identity = 178/207 (85.99%), Postives = 192/207 (92.75%), Query Frame = 1


Query: 1   MEIFWR-AMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNS 60
           ME +W+ AMEQI WYTGLSPTAFFTILA+MIFVFQMVSSMFVSPEEFNKPPTV +SSSNS
Sbjct: 1   MEFYWKSAMEQITWYTGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVAISSSNS 60

Query: 61  ANSNLFVDDSVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGP 120
           +NSNLFV+DS ADAS +VQVGRLTEQQLRAY+GSDPNKPLLMAIKGQIYDVSSGR FYGP
Sbjct: 61  SNSNLFVNDSGADASLAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGP 120

Query: 121 GSPYSMFVGKDASRALALLSFKPEDINGNLEDLNEEELVILQDWEYKFMEKYVKVGELVS 180
           GSPYSMFVGKDASRALALLSFKPEDINGN+E LNEEELVILQDWEYKFMEKYVKVGELV 
Sbjct: 121 GSPYSMFVGKDASRALALLSFKPEDINGNIEGLNEEELVILQDWEYKFMEKYVKVGELVP 180

Query: 181 EEALNERSENGHQSTDTNREEEPNKDQ 207
           E  LNE SENGHQ+++T +EE  +KDQ
Sbjct: 181 EGELNEHSENGHQNSETTQEEH-DKDQ 206

[Close]

BLAST of CmoCh06G000510.1 vs. NCBI nr
Match: gi|703155952|ref|XP_010111338.1| (Membrane steroid-binding protein 2 [Morus notabilis])

HSP 1 Score: 231.9 bits (590), Expect = 1.0e-57
Identity = 119/182 (65.38%), Postives = 144/182 (79.12%), Query Frame = 1


Query: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60
           M I+  A+E+I WYTGLSP AFFTI A M+ V++ VSSMFVSPE+FNKPP        S 
Sbjct: 1   MGIYSAAVEEITWYTGLSPAAFFTIAALMVVVYKTVSSMFVSPEDFNKPPVAMSIEKISQ 60

Query: 61  NSNLFVDDSVADA--SQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYG 120
           +++ F+++ +A    SQ +Q+G +TEQQLRAY+GSDPNKPLL++IKGQIYDVSS R FYG
Sbjct: 61  SNSDFLNNVMATTTNSQPLQLGDMTEQQLRAYNGSDPNKPLLISIKGQIYDVSSSRMFYG 120

Query: 121 PGSPYSMFVGKDASRALALLSFKPEDINGNLEDLNEEELVILQDWEYKFMEKYVKVGELV 180
           PG  YSMF GK+ASRALALLSFKP+DINGNLEDL  EEL +L+DWE KF EKY KVG+LV
Sbjct: 121 PGGAYSMFTGKEASRALALLSFKPQDINGNLEDLGPEELQVLEDWEDKFTEKYAKVGQLV 180

[Close]

BLAST of CmoCh06G000510.1 vs. NCBI nr
Match: gi|356571342|ref|XP_003553837.1| (PREDICTED: membrane steroid-binding protein 2-like [Glycine max])

HSP 1 Score: 231.5 bits (589), Expect = 1.3e-57
Identity = 122/201 (60.70%), Postives = 151/201 (75.12%), Query Frame = 1


Query: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60
           M ++   ME+I++YTGLSP AFFTILA M+ V++ VS MFVSPE++NKPP V    S  A
Sbjct: 1   MGLYTSVMEEISFYTGLSPAAFFTILAMMVVVYRTVSGMFVSPEDYNKPPVV----SARA 60

Query: 61  NSNLFVDDSVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG 120
           NS L   D      + VQ+G +T+++LRAYDGSDP+KPLLMAIKGQIYDVS+GR FYGPG
Sbjct: 61  NSRL---DESEPPREPVQLGEITDRELRAYDGSDPSKPLLMAIKGQIYDVSNGRNFYGPG 120

Query: 121 SPYSMFVGKDASRALALLSFKPEDINGNLEDLNEEELVILQDWEYKFMEKYVKVGELVSE 180
            PY+MF GK+ SRALALLSFKP+DINGNLE L EEEL IL+DWE+KF+EKY KVG+L++E
Sbjct: 121 GPYAMFAGKECSRALALLSFKPDDINGNLEGLGEEELTILEDWEFKFIEKYPKVGQLIAE 180

Query: 181 EAL--NERSENGHQSTDTNRE 200
           +    NE  E    + D   E
Sbjct: 181 QRTRQNEFKEQTQDNLDNPNE 194

[Close]

BLAST of CmoCh06G000510.1 vs. NCBI nr
Match: gi|1012324279|gb|KYP36297.1| (Membrane steroid-binding protein 2 [Cajanus cajan])

HSP 1 Score: 224.9 bits (572), Expect = 1.3e-55
Identity = 122/206 (59.22%), Postives = 152/206 (73.79%), Query Frame = 1


Query: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60
           M ++   ME+I+ YTGLSPTAFFTILA M+ V++ VS+MFVSP+++NKPP V    S  A
Sbjct: 1   MGLYTSFMEEISLYTGLSPTAFFTILAMMVVVYRTVSAMFVSPQDYNKPPVV----SARA 60

Query: 61  NSNLFVDDSVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG 120
           +      D      + V +G +T+++LR+YDGSDPNKPLLMAIKGQIYDVS+GR FYGPG
Sbjct: 61  HPRF---DQPQPPREPVHLGEVTDRELRSYDGSDPNKPLLMAIKGQIYDVSTGRNFYGPG 120

Query: 121 SPYSMFVGKDASRALALLSFKPEDINGNLEDLNEEELVILQDWEYKFMEKYVKVGELVSE 180
            PY+MF GK+ SRALALLSFKPEDINGNLE L E EL IL+DWE+KF+EKY KVG+LVSE
Sbjct: 121 GPYAMFAGKECSRALALLSFKPEDINGNLEGLEESELTILEDWEFKFIEKYPKVGQLVSE 180

Query: 181 EAL--NERSENGHQSTDTNREEEPNK 205
           +    NE  E+   + D N  +E  K
Sbjct: 181 QRTQQNEVKEHIQDNLDPNECKEETK 199

The following BLAST results are available for this feature:

BLAST of CmoCh06G000510.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
MSBP2_ARATH	3.9e-41	47.96	Membrane steroid-binding protein 2 OS=Arabidopsis thaliana GN=MSBP2 PE=1 SV=1	[more]
MSBP1_ARATH	1.1e-38	49.71	Membrane steroid-binding protein 1 OS=Arabidopsis thaliana GN=MSBP1 PE=1 SV=2	[more]
SBP3_ARATH	4.8e-23	52.58	Probable steroid-binding protein 3 OS=Arabidopsis thaliana GN=MP3 PE=1 SV=1	[more]
NEUFC_DANRE	1.7e-15	32.47	Neuferricin OS=Danio rerio GN=cyb5d2 PE=3 SV=1	[more]
DAP1_SCHPO	1.1e-14	45.19	Cytochrome P450 regulator dap1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 2...	[more]

BLAST of CmoCh06G000510.1 vs. TrEMBL


Match Name	E-value	Identity	Description
E5GBX8_CUCME	1.4e-90	85.99	Steroid binding protein OS=Cucumis melo subsp. melo PE=3 SV=1	[more]
A0A0A0LC44_CUCSA	2.4e-90	85.99	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G815470 PE=3 SV=1	[more]
W9S7V8_9ROSA	7.1e-58	65.38	Membrane steroid-binding protein 2 OS=Morus notabilis GN=L484_027995 PE=3 SV=1	[more]
A0A0R0EIY9_SOYBN	9.3e-58	60.70	Uncharacterized protein OS=Glycine max GN=GLYMA_19G064800 PE=3 SV=1	[more]
A0A0B2SJ61_GLYSO	9.3e-58	60.70	Membrane steroid-binding protein 2 OS=Glycine soja GN=glysoja_049787 PE=3 SV=1	[more]

BLAST of CmoCh06G000510.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G48890.1	2.2e-42	47.96	membrane-associated progesterone binding protein 3	[more]
AT5G52240.1	6.0e-40	49.71	membrane steroid binding protein 1	[more]
AT2G24940.1	2.7e-24	52.58	membrane-associated progesterone binding protein 2	[more]
AT4G14965.1	9.6e-14	31.03	membrane-associated progesterone binding protein 4	[more]

BLAST of CmoCh06G000510.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659084981\|ref\|XP_008443179.1\|	2.0e-90	85.99	PREDICTED: membrane steroid-binding protein 2-like [Cucumis melo]	[more]
gi\|449437656\|ref\|XP_004136607.1\|	3.5e-90	85.99	PREDICTED: membrane steroid-binding protein 2-like [Cucumis sativus]	[more]
gi\|703155952\|ref\|XP_010111338.1\|	1.0e-57	65.38	Membrane steroid-binding protein 2 [Morus notabilis]	[more]
gi\|356571342\|ref\|XP_003553837.1\|	1.3e-57	60.70	PREDICTED: membrane steroid-binding protein 2-like [Glycine max]	[more]
gi\|1012324279\|gb\|KYP36297.1\|	1.3e-55	59.22	Membrane steroid-binding protein 2 [Cajanus cajan]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR001199	Cyt_B5-like_heme/steroid-bd

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016491	oxidoreductase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmoCh06G000510	CmoCh06G000510	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmoCh06G000510.1	CmoCh06G000510.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh06G000510.1.exon.2	CmoCh06G000510.1.exon.2	exon
CmoCh06G000510.1.exon.1	CmoCh06G000510.1.exon.1	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh06G000510.1.five_prime_UTR.1	CmoCh06G000510.1.five_prime_UTR.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh06G000510.1.CDS.1	CmoCh06G000510.1.CDS.1	CDS
CmoCh06G000510.1.CDS.2	CmoCh06G000510.1.CDS.2	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmoCh06G000510.1.three_prime_UTR.1	CmoCh06G000510.1.three_prime_UTR.1	three_prime_UTR

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	GENE3D	G3DSA:3.10.120.10		coord: 81..178 score: 2.1
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 83..177 score: 1.5
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 82..178 score: 7.3
IPR001199	Cytochrome b5-like heme/steroid binding domain	unknown	SSF55856	Cytochrome b5-like heme/steroid binding domain	coord: 81..178 score: 1.16
None	No IPR available	PANTHER	PTHR10281	MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED	coord: 1..195 score: 4.9
None	No IPR available	PANTHER	PTHR10281:SF40	SUBFAMILY NOT NAMED	coord: 1..195 score: 4.9