CmaCh20G005860.1 (mRNA) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh20G005860.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Cathepsin B-like cysteine proteinase
Location	Cma_Chr20 : 2780924 .. 2782500 (-)
Sequence length	1020

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGCATATAAAACGATTTGGAACATGGGTTTAACGTCCCTGATTCTCTGGGTTATCTGCACACCCTCAATGGCATCCATGGCAACGGACAGCCCCTCCAATGGCTTACAAGACAGGTACAAGAAATGGATGAATAAACACAGCCGAGAATACAAGAGCAGAGAAGAGCAGGAACGGAGATTCACAGTTTATCAGCTGAATGTTCAGTACATTGACAACTTCAATTCACTGAATCATTCATATACTCTTGCTGAAAATAGTTTTGCAGACCTCACAAATGATGAGTTTAAGATCACTTATTTGGGGTATCAAACTGATTGCCTGTCTGATACATGCTTCAGATATGATCATGTTATTAGCTTGCCTAATCATGTTGACTGGAGAATGGAAGATGCTGTTACTCCGGTAAAGGATCAAGGCCAATGCGGTATGTGTTAGGAATAACAAACCTCCACAATAGTATGCTTTAGGACTTTTCAAAAGGTCTCATACCAATGGAGATGTTTTCTATACTTATAAACCCATGATCATTCTATAAATTAGCCAAGGTGAGACTCTCCCAACAATTTTCCCCTCATACGAAGTACACTATAGAGTCTCCCGTGAGGCCTATGGAGCCCTGGAACAGTTTCCCCTTAATTGAGACTCAACTTCTTTCTCTAGAGTCCTGGAACAAAGTGCACCCTTTTATTCAACAATTGAGTCACTTTTGACTATACCTTCGAGGCTCACAACTACTTTGTTCGATATTTGAGAATTCTATTGACAGCTAAGTTAAGAGCATAGCTCTATACCATGTTAGGAATAACGAACCTTCACAGTAGTATGATATTGTCTACTTTGAGCATAAGCTCTCGTACCTTTACTTTGGACTTCCCCAAAAGGCCATGAACATTTCTTTAATTAGCCAACGTAAAATGAACTCATTCATTAAAACAACAGCCCTTTTATCTATACTCACAAGTTTTGAAGTATTTGAAACAGGGAGTTGCTGGGCATTCTCTGCAGTAGCAGCAGTGGAAGGCATCCACAAAATAAGAACAGGAAAGTTAGAGTCTTTATCAGAGCAAGAGCTTGTGGACTGTGATATCACCTTGGGGAACCAGGGCTGCGATGGCGGTTTCATGAACAAAGCGTTTGAGTACATCAAGAGAAGTGGGCTGACAACAGAGAGAGAATATCCATACAGAGGAATTGAAGCTTTTTGCAACACGCAAAAAGTGAGATACCACTCTGTGACAATAAGTGGGTATGAAAAAGTACCTATGAATAACGAGAAGAAATTGAAAGCTGCTGTTGCTCATCAGCCAGTTTCTGTAGCCATTGATGCAGGGGGATATGATTTTCAATTCTATTCTAGTGGTATCTTCTCAGGTAGCTGTGGGAAGCAGCTCAATCATGGAGTAGCAATCGTTGGGTATGGGGAAGTTGGGGATAATACTTACTGGCTTGTCAAGAATTCGTGGGGGACTGAGTGGGGTGAATCTGGGTACATAAGGATGAAGCGTGATTCGATTGACAAGCGAGGTGCCTGTGGCATAGCCATGGAGGCGAGCTACCCGATCAAAGACTGA

mRNA sequence

ATGGAAGCATATAAAACGATTTGGAACATGGGTTTAACGTCCCTGATTCTCTGGGTTATCTGCACACCCTCAATGGCATCCATGGCAACGGACAGCCCCTCCAATGGCTTACAAGACAGGTACAAGAAATGGATGAATAAACACAGCCGAGAATACAAGAGCAGAGAAGAGCAGGAACGGAGATTCACAGTTTATCAGCTGAATGTTCAGTACATTGACAACTTCAATTCACTGAATCATTCATATACTCTTGCTGAAAATAGTTTTGCAGACCTCACAAATGATGAGTTTAAGATCACTTATTTGGGGTATCAAACTGATTGCCTGTCTGATACATGCTTCAGATATGATCATGTTATTAGCTTGCCTAATCATGTTGACTGGAGAATGGAAGATGCTGTTACTCCGGTAAAGGATCAAGGCCAATGCGGGAGTTGCTGGGCATTCTCTGCAGTAGCAGCAGTGGAAGGCATCCACAAAATAAGAACAGGAAAGTTAGAGTCTTTATCAGAGCAAGAGCTTGTGGACTGTGATATCACCTTGGGGAACCAGGGCTGCGATGGCGGTTTCATGAACAAAGCGTTTGAGTACATCAAGAGAAGTGGGCTGACAACAGAGAGAGAATATCCATACAGAGGAATTGAAGCTTTTTGCAACACGCAAAAAGTGAGATACCACTCTGTGACAATAAGTGGGTATGAAAAAGTACCTATGAATAACGAGAAGAAATTGAAAGCTGCTGTTGCTCATCAGCCAGTTTCTGTAGCCATTGATGCAGGGGGATATGATTTTCAATTCTATTCTAGTGGTATCTTCTCAGGTAGCTGTGGGAAGCAGCTCAATCATGGAGTAGCAATCGTTGGGTATGGGGAAGTTGGGGATAATACTTACTGGCTTGTCAAGAATTCGTGGGGGACTGAGTGGGGTGAATCTGGGTACATAAGGATGAAGCGTGATTCGATTGACAAGCGAGGTGCCTGTGGCATAGCCATGGAGGCGAGCTACCCGATCAAAGACTGA

Coding sequence (CDS)

ATGGAAGCATATAAAACGATTTGGAACATGGGTTTAACGTCCCTGATTCTCTGGGTTATCTGCACACCCTCAATGGCATCCATGGCAACGGACAGCCCCTCCAATGGCTTACAAGACAGGTACAAGAAATGGATGAATAAACACAGCCGAGAATACAAGAGCAGAGAAGAGCAGGAACGGAGATTCACAGTTTATCAGCTGAATGTTCAGTACATTGACAACTTCAATTCACTGAATCATTCATATACTCTTGCTGAAAATAGTTTTGCAGACCTCACAAATGATGAGTTTAAGATCACTTATTTGGGGTATCAAACTGATTGCCTGTCTGATACATGCTTCAGATATGATCATGTTATTAGCTTGCCTAATCATGTTGACTGGAGAATGGAAGATGCTGTTACTCCGGTAAAGGATCAAGGCCAATGCGGGAGTTGCTGGGCATTCTCTGCAGTAGCAGCAGTGGAAGGCATCCACAAAATAAGAACAGGAAAGTTAGAGTCTTTATCAGAGCAAGAGCTTGTGGACTGTGATATCACCTTGGGGAACCAGGGCTGCGATGGCGGTTTCATGAACAAAGCGTTTGAGTACATCAAGAGAAGTGGGCTGACAACAGAGAGAGAATATCCATACAGAGGAATTGAAGCTTTTTGCAACACGCAAAAAGTGAGATACCACTCTGTGACAATAAGTGGGTATGAAAAAGTACCTATGAATAACGAGAAGAAATTGAAAGCTGCTGTTGCTCATCAGCCAGTTTCTGTAGCCATTGATGCAGGGGGATATGATTTTCAATTCTATTCTAGTGGTATCTTCTCAGGTAGCTGTGGGAAGCAGCTCAATCATGGAGTAGCAATCGTTGGGTATGGGGAAGTTGGGGATAATACTTACTGGCTTGTCAAGAATTCGTGGGGGACTGAGTGGGGTGAATCTGGGTACATAAGGATGAAGCGTGATTCGATTGACAAGCGAGGTGCCTGTGGCATAGCCATGGAGGCGAGCTACCCGATCAAAGACTGA

Protein sequence

MEAYKTIWNMGLTSLILWVICTPSMASMATDSPSNGLQDRYKKWMNKHSREYKSREEQERRFTVYQLNVQYIDNFNSLNHSYTLAENSFADLTNDEFKITYLGYQTDCLSDTCFRYDHVISLPNHVDWRMEDAVTPVKDQGQCGSCWAFSAVAAVEGIHKIRTGKLESLSEQELVDCDITLGNQGCDGGFMNKAFEYIKRSGLTTEREYPYRGIEAFCNTQKVRYHSVTISGYEKVPMNNEKKLKAAVAHQPVSVAIDAGGYDFQFYSSGIFSGSCGKQLNHGVAIVGYGEVGDNTYWLVKNSWGTEWGESGYIRMKRDSIDKRGACGIAMEASYPIKD

Homology

[Close]

BLAST of CmaCh20G005860.1 vs. Swiss-Prot
Match: CEP1_ARATH (KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana GN=CEP1 PE=1 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 1.2e-92
Identity = 176/315 (55.87%), Postives = 221/315 (70.16%), Query Frame = 1


Query: 35  NGLQDRYKKWMNKHSREYKSREEQERRFTVYQLNVQYIDNFNSLNHSYTLAENSFADLTN 94
           N L + Y++W + H+   +S EE+ +RF V++ NV++I   N  + SY L  N F D+T+
Sbjct: 32  NSLWELYERWRSHHTVA-RSLEEKAKRFNVFKHNVKHIHETNKKDKSYKLKLNKFGDMTS 91

Query: 95  DEFKITYLG--------YQTDCLSDTCFRYDHVISLPNHVDWRMEDAVTPVKDQGQCGSC 154
           +EF+ TY G        +Q +  +   F Y +V +LP  VDWR   AVTPVK+QGQCGSC
Sbjct: 92  EEFRRTYAGSNIKHHRMFQGEKKATKSFMYANVNTLPTSVDWRKNGAVTPVKNQGQCGSC 151

Query: 155 WAFSAVAAVEGIHKIRTGKLESLSEQELVDCDITLGNQGCDGGFMNKAFEYIK-RSGLTT 214
           WAFS V AVEGI++IRT KL SLSEQELVDCD T  NQGC+GG M+ AFE+IK + GLT+
Sbjct: 152 WAFSTVVAVEGINQIRTKKLTSLSEQELVDCD-TNQNQGCNGGLMDLAFEFIKEKGGLTS 211

Query: 215 EREYPYRGIEAFCNTQKVRYHSVTISGYEKVPMNNEKKLKAAVAHQPVSVAIDAGGYDFQ 274
           E  YPY+  +  C+T K     V+I G+E VP N+E  L  AVA+QPVSVAIDAGG DFQ
Sbjct: 212 ELVYPYKASDETCDTNKENAPVVSIDGHEDVPKNSEDDLMKAVANQPVSVAIDAGGSDFQ 271

Query: 275 FYSSGIFSGSCGKQLNHGVAIVGYGEVGDNT-YWLVKNSWGTEWGESGYIRMKRDSIDKR 334
           FYS G+F+G CG +LNHGVA+VGYG   D T YW+VKNSWG EWGE GYIRM+R    K 
Sbjct: 272 FYSEGVFTGRCGTELNHGVAVVGYGTTIDGTKYWIVKNSWGEEWGEKGYIRMQRGIRHKE 331

Query: 335 GACGIAMEASYPIKD 340
           G CGIAMEASYP+K+
Sbjct: 332 GLCGIAMEASYPLKN 344

[Close]

BLAST of CmaCh20G005860.1 vs. Swiss-Prot
Match: CYSEP_RICCO (Vignain OS=Ricinus communis GN=CYSEP PE=1 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 8.0e-92
Identity = 172/308 (55.84%), Postives = 215/308 (69.81%), Query Frame = 1


Query: 41  YKKWMNKHSREYKSREEQERRFTVYQLNVQYIDNFNSLNHSYTLAENSFADLTNDEFKIT 100
           Y++W + H+   +S  E+++RF V++ N  ++ N N ++  Y L  N FAD+TN EF+ T
Sbjct: 38  YERWRSHHTVS-RSLHEKQKRFNVFKHNAMHVHNANKMDKPYKLKLNKFADMTNHEFRNT 97

Query: 101 YLG--------YQTDCLSDTCFRYDHVISLPNHVDWRMEDAVTPVKDQGQCGSCWAFSAV 160
           Y G        ++     +  F Y+ V ++P  VDWR + AVT VKDQGQCGSCWAFS +
Sbjct: 98  YSGSKVKHHRMFRGGPRGNGTFMYEKVDTVPASVDWRKKGAVTSVKDQGQCGSCWAFSTI 157

Query: 161 AAVEGIHKIRTGKLESLSEQELVDCDITLGNQGCDGGFMNKAFEYIK-RSGLTTEREYPY 220
            AVEGI++I+T KL SLSEQELVDCD T  NQGC+GG M+ AFE+IK R G+TTE  YPY
Sbjct: 158 VAVEGINQIKTNKLVSLSEQELVDCD-TDQNQGCNGGLMDYAFEFIKQRGGITTEANYPY 217

Query: 221 RGIEAFCNTQKVRYHSVTISGYEKVPMNNEKKLKAAVAHQPVSVAIDAGGYDFQFYSSGI 280
              +  C+  K    +V+I G+E VP N+E  L  AVA+QPVSVAIDAGG DFQFYS G+
Sbjct: 218 EAYDGTCDVSKENAPAVSIDGHENVPENDENALLKAVANQPVSVAIDAGGSDFQFYSEGV 277

Query: 281 FSGSCGKQLNHGVAIVGYGEVGDNT-YWLVKNSWGTEWGESGYIRMKRDSIDKRGACGIA 339
           F+GSCG +L+HGVAIVGYG   D T YW VKNSWG EWGE GYIRM+R   DK G CGIA
Sbjct: 278 FTGSCGTELDHGVAIVGYGTTIDGTKYWTVKNSWGPEWGEKGYIRMERGISDKEGLCGIA 337

[Close]

BLAST of CmaCh20G005860.1 vs. Swiss-Prot
Match: CYSEP_VIGMU (Vignain OS=Vigna mungo PE=1 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 1.4e-91
Identity = 180/349 (51.58%), Postives = 228/349 (65.33%), Query Frame = 1


Query: 1   MEAYKTIWNMGLTSLILWVICTPSMASMATDSPSNGLQDRYKKWMNKHSREYKSREEQER 60
           M   K +W +   SL+L V  +        +S  + L D Y++W + H+   +S  E+ +
Sbjct: 1   MAMKKLLWVVLSLSLVLGVANSFDFHEKDLESEES-LWDLYERWRSHHTVS-RSLGEKHK 60

Query: 61  RFTVYQLNVQYIDNFNSLNHSYTLAENSFADLTNDEFKITYLG--------YQTDCLSDT 120
           RF V++ NV ++ N N ++  Y L  N FAD+TN EF+ TY G        ++       
Sbjct: 61  RFNVFKANVMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHHKMFRGSQHGSG 120

Query: 121 CFRYDHVISLPNHVDWRMEDAVTPVKDQGQCGSCWAFSAVAAVEGIHKIRTGKLESLSEQ 180
            F Y+ V S+P  VDWR + AVT VKDQGQCGSCWAFS + AVEGI++I+T KL SLSEQ
Sbjct: 121 TFMYEKVGSVPASVDWRKKGAVTDVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQ 180

Query: 181 ELVDCDITLGNQGCDGGFMNKAFEYIK-RSGLTTEREYPYRGIEAFCNTQKVRYHSVTIS 240
           ELVDCD    NQGC+GG M  AFE+IK + G+TTE  YPY   E  C+  KV   +V+I 
Sbjct: 181 ELVDCDKE-ENQGCNGGLMESAFEFIKQKGGITTESNYPYTAQEGTCDESKVNDLAVSID 240

Query: 241 GYEKVPMNNEKKLKAAVAHQPVSVAIDAGGYDFQFYSSGIFSGSCGKQLNHGVAIVGYGE 300
           G+E VP+N+E  L  AVA+QPVSVAIDAGG DFQFYS G+F+G C   LNHGVAIVGYG 
Sbjct: 241 GHENVPVNDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGDCNTDLNHGVAIVGYGT 300

Query: 301 VGDNT-YWLVKNSWGTEWGESGYIRMKRDSIDKRGACGIAMEASYPIKD 340
             D T YW+V+NSWG EWGE GYIRM+R+   K G CGIAM ASYPIK+
Sbjct: 301 TVDGTNYWIVRNSWGPEWGEQGYIRMQRNISKKEGLCGIAMMASYPIKN 346

[Close]

BLAST of CmaCh20G005860.1 vs. Swiss-Prot
Match: CEP2_ARATH (KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana GN=CEP2 PE=1 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 2.3e-91
Identity = 175/314 (55.73%), Postives = 214/314 (68.15%), Query Frame = 1


Query: 36  GLQDRYKKWMNKHSREYKSREEQERRFTVYQLNVQYIDNFNSLNHSYTLAENSFADLTND 95
           GL   Y +W + HS   +S  E+E+RF V++ NV ++ N N  N SY L  N FADLT +
Sbjct: 33  GLSTLYDRWRSHHSVP-RSLNEREKRFNVFRHNVMHVHNTNKKNRSYKLKLNKFADLTIN 92

Query: 96  EFKITYLG--------YQTDCLSDTCFRYDH--VISLPNHVDWRMEDAVTPVKDQGQCGS 155
           EFK  Y G         Q        F YDH  +  LP+ VDWR + AVT +K+QG+CGS
Sbjct: 93  EFKNAYTGSNIKHHRMLQGPKRGSKQFMYDHENLSKLPSSVDWRKKGAVTEIKNQGKCGS 152

Query: 156 CWAFSAVAAVEGIHKIRTGKLESLSEQELVDCDITLGNQGCDGGFMNKAFEYIKRSG-LT 215
           CWAFS VAAVEGI+KI+T KL SLSEQELVDCD T  N+GC+GG M  AFE+IK++G +T
Sbjct: 153 CWAFSTVAAVEGINKIKTNKLVSLSEQELVDCD-TKQNEGCNGGLMEIAFEFIKKNGGIT 212

Query: 216 TEREYPYRGIEAFCNTQKVRYHSVTISGYEKVPMNNEKKLKAAVAHQPVSVAIDAGGYDF 275
           TE  YPY GI+  C+  K     VTI G+E VP N+E  L  AVA+QPVSVAIDAG  DF
Sbjct: 213 TEDSYPYEGIDGKCDASKDNGVLVTIDGHEDVPENDENALLKAVANQPVSVAIDAGSSDF 272

Query: 276 QFYSSGIFSGSCGKQLNHGVAIVGYGEVGDNTYWLVKNSWGTEWGESGYIRMKRDSIDKR 335
           QFYS G+F+GSCG +LNHGVA VGYG      YW+V+NSWG EWGE GYI+++R+  +  
Sbjct: 273 QFYSEGVFTGSCGTELNHGVAAVGYGSERGKKYWIVRNSWGAEWGEGGYIKIEREIDEPE 332

Query: 336 GACGIAMEASYPIK 339
           G CGIAMEASYPIK
Sbjct: 333 GRCGIAMEASYPIK 344

[Close]

BLAST of CmaCh20G005860.1 vs. Swiss-Prot
Match: CYSEP_PHAVU (Vignain OS=Phaseolus vulgaris PE=2 SV=2)

HSP 1 Score: 336.7 bits (862), Expect = 3.0e-91
Identity = 176/346 (50.87%), Postives = 226/346 (65.32%), Query Frame = 1


Query: 13  TSLILWVICTPSMASMATDS---------PSNGLQDRYKKWMNKHSREYKSREEQERRFT 72
           T  +LWV+ + S+     +S             L D Y++W + H+   +S  E+ +RF 
Sbjct: 3   TKKLLWVVLSFSLVLGVANSFDFHDKDLASEESLWDLYERWRSHHTVS-RSLGEKHKRFN 62

Query: 73  VYQLNVQYIDNFNSLNHSYTLAENSFADLTNDEFKITYLG--------YQTDCLSDTCFR 132
           V++ N+ ++ N N ++  Y L  N FAD+TN EF+ TY G        ++     +  F 
Sbjct: 63  VFKANLMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHPRMFRGTPHENGAFM 122

Query: 133 YDHVISLPNHVDWRMEDAVTPVKDQGQCGSCWAFSAVAAVEGIHKIRTGKLESLSEQELV 192
           Y+ V+S+P  VDWR + AVT VKDQGQCGSCWAFS V AVEGI++I+T KL +LSEQELV
Sbjct: 123 YEKVVSVPPSVDWRKKGAVTDVKDQGQCGSCWAFSTVVAVEGINQIKTNKLVALSEQELV 182

Query: 193 DCDITLGNQGCDGGFMNKAFEYIK-RSGLTTEREYPYRGIEAFCNTQKVRYHSVTISGYE 252
           DCD    NQGC+GG M  AFE+IK + G+TTE  YPY+  E  C+  KV   +V+I G+E
Sbjct: 183 DCDKE-ENQGCNGGLMESAFEFIKQKGGITTESNYPYKAQEGTCDASKVNDLAVSIDGHE 242

Query: 253 KVPMNNEKKLKAAVAHQPVSVAIDAGGYDFQFYSSGIFSGSCGKQLNHGVAIVGYGEVGD 312
            VP N+E  L  AVA+QPVSVAIDAGG DFQFYS G+F+G C   LNHGVAIVGYG   D
Sbjct: 243 NVPANDEDALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGDCSTDLNHGVAIVGYGTTVD 302

Query: 313 NT-YWLVKNSWGTEWGESGYIRMKRDSIDKRGACGIAMEASYPIKD 340
            T YW+V+NSWG EWGE GYIRM+R+   K G CGIAM  SYPIK+
Sbjct: 303 GTNYWIVRNSWGPEWGEHGYIRMQRNISKKEGLCGIAMLPSYPIKN 346

[Close]

BLAST of CmaCh20G005860.1 vs. TrEMBL
Match: A0A0A0LJV6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G292830 PE=3 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 6.9e-143
Identity = 244/336 (72.62%), Postives = 286/336 (85.12%), Query Frame = 1


Query: 9   NMGLTSLILWVICTPSMASMATD-----SPSNGLQDRYKKWMNKHSREYKSREEQERRFT 68
           N+ L  LILWV  TP + SMA D     S S+ +QDRY+KWM+K+ R+YKSREE ERRFT
Sbjct: 5   NVSLIFLILWVFWTPRLVSMAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREEWERRFT 64

Query: 69  VYQLNVQYIDNFNSLNHSYTLAENSFADLTNDEFKITYLGYQTDCLSDTCFRYDHVISLP 128
           +YQ NVQYIDNFNS+NHS+TLAEN+FADLTN+EFK TYLGY+T  + DTCFRY ++++LP
Sbjct: 65  IYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYGNMVNLP 124

Query: 129 NHVDWRMEDAVTPVKDQGQCGSCWAFSAVAAVEGIHKIRTGKLESLSEQELVDCDITLGN 188
            +VDWR E AVTP+K+QGQCGSCWAFSAVAAVEGI+KI+ GKL SLSEQELVDCD+T GN
Sbjct: 125 TNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCDVTSGN 184

Query: 189 QGCDGGFMNKAFEYIKRSGLTTEREYPYRGIEAFCNTQKVRYHSVTISGYEKVPMNNEKK 248
           QGC+GG+M KAFE+IKR+GLTTE EYPY+G E+ CN QK +Y  V+ISGYEKVP+N+EK 
Sbjct: 185 QGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNEQKEKYQFVSISGYEKVPVNDEKS 244

Query: 249 LKAAVAHQPVSVAIDAGGYDFQFYSSGIFSGSCGKQLNHGVAIVGYGEVGDNTYWLVKNS 308
           LKAAVA+QPVSVAIDA G +FQFYS GIFSG+CG QLNHGVAIVGYGE  +  YWLVKNS
Sbjct: 245 LKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQAYWLVKNS 304

Query: 309 WGTEWGESGYIRMKRDSIDKRGACGIAMEASYPIKD 340
           WGT+WGESGYIRMKRDS D++G CGIAM ASYP KD
Sbjct: 305 WGTDWGESGYIRMKRDSTDRQGTCGIAMMASYPTKD 340

[Close]

BLAST of CmaCh20G005860.1 vs. TrEMBL
Match: M5X1M2_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023515mg PE=3 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 2.8e-115
Identity = 197/342 (57.60%), Postives = 250/342 (73.10%), Query Frame = 1


Query: 1   MEAYKTIWNMGLTSLILWVICTPSMASMATDSP----SNGLQDRYKKWMNKHSREYKSRE 60
           ME    +    LT L++W+ C  S A   T  P       +++RY++W+ K+ R YK+RE
Sbjct: 1   METSMVLTRASLTFLMVWIFCISSTACSETYKPLRTDPKAMKERYERWLQKYGRIYKNRE 60

Query: 61  EQERRFTVYQLNVQYIDNFNSLNHSYTLAENSFADLTNDEFKITYLGYQTDCLSDTCFRY 120
           E   RF VY+ N++++D  NS N SY L +N FAD+TN EF  T++G+QT     T F Y
Sbjct: 61  EAAYRFGVYKSNIEFVDFVNSQNLSYKLTDNKFADITNLEFTKTFMGFQTRSHPKTKFSY 120

Query: 121 DHVISLPNHVDWRMEDAVTPVKDQGQCGSCWAFSAVAAVEGIHKIRTGKLESLSEQELVD 180
           D    LP  VDWR   AVTP+K+QGQCGSCWAFSAVAAVEGI++I+TGKL SLSEQELVD
Sbjct: 121 DKDEELPTAVDWRKHGAVTPIKNQGQCGSCWAFSAVAAVEGINQIKTGKLVSLSEQELVD 180

Query: 181 CDITLGNQGCDGGFMNKAFEYIKRSGLTTEREYPYRGIEAFCNTQKVRYHSVTISGYEKV 240
           CD+  GN+GC+GG+M KAF +IK +GL+TE++YPY+G +  C+   ++  +V ISGYE +
Sbjct: 181 CDVKTGNEGCNGGYMEKAFSFIKDNGLSTEKDYPYKGSDGICDEDSLKNSAVNISGYESI 240

Query: 241 PMNNEKKLKAAVAHQPVSVAIDAGGYDFQFYSSGIFSGSCGKQLNHGVAIVGYGEVGDNT 300
           P N+EK L+AAVAHQPVSVA+DA GY FQFYSSG F+G CGK LNHGV  VGYGE     
Sbjct: 241 PANSEKSLQAAVAHQPVSVAVDAAGYAFQFYSSGTFTGQCGKNLNHGVTAVGYGEDSGKK 300

Query: 301 YWLVKNSWGTEWGESGYIRMKRDSIDKRGACGIAMEASYPIK 339
           YW+VKNSWG +WGESGYIRM RDS+DK+G CGIAM+ASYP+K
Sbjct: 301 YWIVKNSWGPDWGESGYIRMTRDSVDKKGTCGIAMQASYPVK 342

[Close]

BLAST of CmaCh20G005860.1 vs. TrEMBL
Match: G7ZUL7_MEDTR (Cysteine proteinase OS=Medicago truncatula GN=MTR_2g090890 PE=3 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 5.4e-111
Identity = 198/337 (58.75%), Postives = 248/337 (73.59%), Query Frame = 1


Query: 5   KTIWNMGLTSLILWVICT--PSMASMATDSPSNGLQDRYKKWMNKHSREYKSREEQERRF 64
           KT   + +  L LW+I +  P + +  + +P+  ++ RY+ W+ ++ R Y+ REE E RF
Sbjct: 2   KTTITLSIVILNLWIIASACPEIHTKNSTNPAV-MKKRYETWLKRYGRHYRDREEWEVRF 61

Query: 65  TVYQLNVQYIDNFNSLNHSYTLAENSFADLTNDEFKITYLGYQTDCLSDTCFRYDHVISL 124
            +YQ NVQYI+ +NS N+SY L +N FAD+TN+EFK TYLGY       T FRY     L
Sbjct: 62  DIYQSNVQYIEFYNSQNYSYKLIDNRFADITNEEFKSTYLGYLPRFRVQTEFRYHKHGEL 121

Query: 125 PNHVDWRMEDAVTPVKDQGQCGSCWAFSAVAAVEGIHKIRTGKLESLSEQELVDCDITLG 184
           P  +DWR + AVT VKDQG+CGSCWAFSAVAAVEGI+KI+T  L SLSEQ+L+DCDI  G
Sbjct: 122 PKSIDWRKKGAVTHVKDQGRCGSCWAFSAVAAVEGINKIKTENLVSLSEQQLIDCDIKSG 181

Query: 185 NQGCDGGFMNKAFEYIKR-SGLTTEREYPYRGIEAFCNTQKVRYHSVTISGYEKVPMNNE 244
           N+GC+GG M  AF YIK+  G+ T +EYPY+G +  CN  K + ++VTISGYE VP  NE
Sbjct: 182 NEGCEGGDMYIAFNYIKKHGGIATAKEYPYKGRDGNCNKSKAKNNAVTISGYESVPARNE 241

Query: 245 KKLKAAVAHQPVSVAIDAGGYDFQFYSSGIFSGSCGKQLNHGVAIVGYGEVGDNTYWLVK 304
           K LKAAVAHQPVS+A DAGGY FQFYS GIFSGSCGK LNHG+ IVGYGE   + YW+VK
Sbjct: 242 KMLKAAVAHQPVSIATDAGGYAFQFYSKGIFSGSCGKNLNHGMTIVGYGEENGDKYWIVK 301

Query: 305 NSWGTEWGESGYIRMKRDSIDKRGACGIAMEASYPIK 339
           NSW  +WGESGY+RMKRD+ DK G CGIAM+A+YP+K
Sbjct: 302 NSWANDWGESGYVRMKRDTKDKDGTCGIAMDATYPVK 337

[Close]

BLAST of CmaCh20G005860.1 vs. TrEMBL
Match: W9RAD3_9ROSA (KDEL-tailed cysteine endopeptidase CEP2 OS=Morus notabilis GN=L484_001450 PE=3 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 1.6e-110
Identity = 195/346 (56.36%), Postives = 244/346 (70.52%), Query Frame = 1


Query: 1   MEAYKTIWNMGLTSLILWVICTPSMASMATDSP-------SNGLQDRYKKWMNKHSREYK 60
           ME    +    L  LIL  +  PS        P          ++ RY +W  ++ R Y 
Sbjct: 1   MEIPIVLRGASLALLILLTLLPPSRVYSTEYRPLWREEHNRQAVRQRYDRWAEQYGRNYG 60

Query: 61  SREEQERRFTVYQLNVQYIDNFNSLNHSYTLAENSFADLTNDEFKITYLGYQTDCLSDTC 120
           S EE+E RF +Y +N+ +I+  NS N SY L +N FAD+ N EF++  LGY+    + T 
Sbjct: 61  SEEEKELRFQIYHMNLLFIEQVNSQNFSYKLTDNKFADMMNAEFRLRLLGYRPLLHNQTS 120

Query: 121 FRYDHVISLPNHVDWRMEDAVTPVKDQGQCGSCWAFSAVAAVEGIHKIRTGKLESLSEQE 180
           FR+   + +P  VDWR   AVTPVKDQGQCGSCWAFS+VAAVEG+++I+TG+L SLSEQE
Sbjct: 121 FRFGGPMLVPKQVDWRKNGAVTPVKDQGQCGSCWAFSSVAAVEGVNQIKTGELVSLSEQE 180

Query: 181 LVDCDITLGNQGCDGGFMNKAFEYIKRSGLTTEREYPYRGIEAFCNTQKVRYHSVTISGY 240
           LVDCD+  GNQGC+GG+M KAF++IKR+G+TT  +YPYRG    C+  K+R   V ISGY
Sbjct: 181 LVDCDVNTGNQGCNGGYMEKAFQFIKRNGITTNGKYPYRGANGRCDEDKLRGRRVKISGY 240

Query: 241 EKVPMNNEKKLKAAVAHQPVSVAIDAGGYDFQFYSSGIFSGSCGKQLNHGVAIVGYGEVG 300
           EKVP N+E++L+A VAHQPVSVAIDAGG +FQFYS GIF+G CG  LNHGV +VGYGE  
Sbjct: 241 EKVPHNDEERLQATVAHQPVSVAIDAGGSEFQFYSHGIFNGRCGTDLNHGVTVVGYGEED 300

Query: 301 DNTYWLVKNSWGTEWGESGYIRMKRDSIDKRGACGIAMEASYPIKD 340
             TYWLVKNSWGTEWGESGY+R+ R S+D RG CGIAMEASYP+KD
Sbjct: 301 GKTYWLVKNSWGTEWGESGYVRIHRGSVDGRGTCGIAMEASYPVKD 346

[Close]

BLAST of CmaCh20G005860.1 vs. TrEMBL
Match: A0A151TPE0_CAJCA (Vignain OS=Cajanus cajan GN=KK1_022504 PE=3 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 2.5e-108
Identity = 190/304 (62.50%), Postives = 238/304 (78.29%), Query Frame = 1


Query: 40  RYKKWMNKHSREYKSREEQERRFTVYQLNVQYIDNFNSLNHSYTLAENSFADLTNDEFKI 99
           R+++W+ +H R YK +EE E RF +YQ N+++I+  NS N+SY L +N FADLTN+EF  
Sbjct: 4   RFERWITQHGRNYKDKEEWEVRFGIYQANLKFIECKNSQNNSYNLIDNKFADLTNEEFMS 63

Query: 100 TYLGYQTDCLSDTCF-RYDHVISLPNHVDWRMEDAVTPVKDQGQCGSCWAFSAVAAVEGI 159
           TYLG+ T   + T    Y H   LP  +DWR E AVT +KDQG CGSCWAFSAVAAVEGI
Sbjct: 64  TYLGFGTRLPTHTGIGSYKHG-DLPESMDWRKEGAVTEIKDQGNCGSCWAFSAVAAVEGI 123

Query: 160 HKIRTGKLESLSEQELVDCDITLGNQGCDGGFMNKAFEYIKRS-GLTTEREYPYRGIEAF 219
           +KI++GKL SLSEQEL+DCD+  GNQGC+GG M+ AF++IKR+ GL T +EYPY G++  
Sbjct: 124 NKIKSGKLVSLSEQELIDCDVENGNQGCEGGLMDTAFKFIKRNGGLATAKEYPYEGVDGT 183

Query: 220 CNTQKVRYHSVTISGYEKVPMNNEKKLKAAVAHQPVSVAIDAGGYDFQFYSSGIFSGSCG 279
           CN +K  +H+V ISGYE+VP NNE  LKAAVA+QPVSVAIDAGGY+FQ YS G+FSG CG
Sbjct: 184 CNKEKAGHHAVNISGYERVPSNNEAMLKAAVANQPVSVAIDAGGYEFQLYSEGVFSGRCG 243

Query: 280 KQLNHGVAIVGYGE--VGDNTYWLVKNSWGTEWGESGYIRMKRDSIDKRGACGIAMEASY 339
           K LNHGV +VGYGE  +GD  YW+VKNSWG +WGESGY+RMKRD++D  G CGIAM+ASY
Sbjct: 244 KHLNHGVTVVGYGEENIGDK-YWIVKNSWGLDWGESGYVRMKRDTVDDAGICGIAMQASY 303

[Close]

BLAST of CmaCh20G005860.1 vs. TAIR10
Match: AT1G06260.1 (AT1G06260.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 349.0 bits (894), Expect = 3.3e-96
Identity = 176/339 (51.92%), Postives = 226/339 (66.67%), Query Frame = 1


Query: 9   NMGLTSLILWVICTPSMASMATD--SPSNGLQDRYKKWMNKHSREYKSREEQERRFTVYQ 68
           N+ L  LI +V+    + S+ +    P   L+ R++KW+  HS+ Y  R+E   RF +YQ
Sbjct: 9   NLTLAVLICFVLIASKLCSVDSSVYDPHKTLKQRFEKWLKTHSKLYGGRDEWMLRFGIYQ 68

Query: 69  LNVQYIDNFNSLNHSYTLAENSFADLTNDEFKITYLGYQTDCLS------DTCFRYDHVI 128
            NVQ ID  NSL+  + L +N FAD+TN EFK  +LG  T  L         C   D   
Sbjct: 69  SNVQLIDYINSLHLPFKLTDNRFADMTNSEFKAHFLGLNTSSLRLHKKQRPVC---DPAG 128

Query: 129 SLPNHVDWRMEDAVTPVKDQGQCGSCWAFSAVAAVEGIHKIRTGKLESLSEQELVDCDIT 188
           ++P+ VDWR + AVTP+++QG+CG CWAFSAVAA+EGI+KI+TG L SLSEQ+L+DCD+ 
Sbjct: 129 NVPDAVDWRTQGAVTPIRNQGKCGGCWAFSAVAAIEGINKIKTGNLVSLSEQQLIDCDVG 188

Query: 189 LGNQGCDGGFMNKAFEYIKRSG-LTTEREYPYRGIEAFCNTQKVRYHSVTISGYEKVPMN 248
             N+GC GG M  AFE+IK +G L TE +YPY GIE  C+ +K +   VTI GY+KV  N
Sbjct: 189 TYNKGCSGGLMETAFEFIKTNGGLATETDYPYTGIEGTCDQEKSKNKVVTIQGYQKVAQN 248

Query: 249 NEKKLKAAVAHQPVSVAIDAGGYDFQFYSSGIFSGSCGKQLNHGVAIVGYGEVGDNTYWL 308
            E  L+ A A QPVSV IDAGG+ FQ YSSG+F+  CG  LNHGV +VGYG  GD  YW+
Sbjct: 249 -EASLQIAAAQQPVSVGIDAGGFIFQLYSSGVFTNYCGTNLNHGVTVVGYGVEGDQKYWI 308

Query: 309 VKNSWGTEWGESGYIRMKRDSIDKRGACGIAMEASYPIK 339
           VKNSWGT WGE GYIRM+R   +  G CGIAM ASYP++
Sbjct: 309 VKNSWGTGWGEEGYIRMERGVSEDTGKCGIAMMASYPLQ 343

[Close]

BLAST of CmaCh20G005860.1 vs. TAIR10
Match: AT5G50260.1 (AT5G50260.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 341.3 bits (874), Expect = 7.0e-94
Identity = 176/315 (55.87%), Postives = 221/315 (70.16%), Query Frame = 1


Query: 35  NGLQDRYKKWMNKHSREYKSREEQERRFTVYQLNVQYIDNFNSLNHSYTLAENSFADLTN 94
           N L + Y++W + H+   +S EE+ +RF V++ NV++I   N  + SY L  N F D+T+
Sbjct: 32  NSLWELYERWRSHHTVA-RSLEEKAKRFNVFKHNVKHIHETNKKDKSYKLKLNKFGDMTS 91

Query: 95  DEFKITYLG--------YQTDCLSDTCFRYDHVISLPNHVDWRMEDAVTPVKDQGQCGSC 154
           +EF+ TY G        +Q +  +   F Y +V +LP  VDWR   AVTPVK+QGQCGSC
Sbjct: 92  EEFRRTYAGSNIKHHRMFQGEKKATKSFMYANVNTLPTSVDWRKNGAVTPVKNQGQCGSC 151

Query: 155 WAFSAVAAVEGIHKIRTGKLESLSEQELVDCDITLGNQGCDGGFMNKAFEYIK-RSGLTT 214
           WAFS V AVEGI++IRT KL SLSEQELVDCD T  NQGC+GG M+ AFE+IK + GLT+
Sbjct: 152 WAFSTVVAVEGINQIRTKKLTSLSEQELVDCD-TNQNQGCNGGLMDLAFEFIKEKGGLTS 211

Query: 215 EREYPYRGIEAFCNTQKVRYHSVTISGYEKVPMNNEKKLKAAVAHQPVSVAIDAGGYDFQ 274
           E  YPY+  +  C+T K     V+I G+E VP N+E  L  AVA+QPVSVAIDAGG DFQ
Sbjct: 212 ELVYPYKASDETCDTNKENAPVVSIDGHEDVPKNSEDDLMKAVANQPVSVAIDAGGSDFQ 271

Query: 275 FYSSGIFSGSCGKQLNHGVAIVGYGEVGDNT-YWLVKNSWGTEWGESGYIRMKRDSIDKR 334
           FYS G+F+G CG +LNHGVA+VGYG   D T YW+VKNSWG EWGE GYIRM+R    K 
Sbjct: 272 FYSEGVFTGRCGTELNHGVAVVGYGTTIDGTKYWIVKNSWGEEWGEKGYIRMQRGIRHKE 331

Query: 335 GACGIAMEASYPIKD 340
           G CGIAMEASYP+K+
Sbjct: 332 GLCGIAMEASYPLKN 344

[Close]

BLAST of CmaCh20G005860.1 vs. TAIR10
Match: AT3G48340.1 (AT3G48340.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 337.0 bits (863), Expect = 1.3e-92
Identity = 175/314 (55.73%), Postives = 214/314 (68.15%), Query Frame = 1


Query: 36  GLQDRYKKWMNKHSREYKSREEQERRFTVYQLNVQYIDNFNSLNHSYTLAENSFADLTND 95
           GL   Y +W + HS   +S  E+E+RF V++ NV ++ N N  N SY L  N FADLT +
Sbjct: 33  GLSTLYDRWRSHHSVP-RSLNEREKRFNVFRHNVMHVHNTNKKNRSYKLKLNKFADLTIN 92

Query: 96  EFKITYLG--------YQTDCLSDTCFRYDH--VISLPNHVDWRMEDAVTPVKDQGQCGS 155
           EFK  Y G         Q        F YDH  +  LP+ VDWR + AVT +K+QG+CGS
Sbjct: 93  EFKNAYTGSNIKHHRMLQGPKRGSKQFMYDHENLSKLPSSVDWRKKGAVTEIKNQGKCGS 152

Query: 156 CWAFSAVAAVEGIHKIRTGKLESLSEQELVDCDITLGNQGCDGGFMNKAFEYIKRSG-LT 215
           CWAFS VAAVEGI+KI+T KL SLSEQELVDCD T  N+GC+GG M  AFE+IK++G +T
Sbjct: 153 CWAFSTVAAVEGINKIKTNKLVSLSEQELVDCD-TKQNEGCNGGLMEIAFEFIKKNGGIT 212

Query: 216 TEREYPYRGIEAFCNTQKVRYHSVTISGYEKVPMNNEKKLKAAVAHQPVSVAIDAGGYDF 275
           TE  YPY GI+  C+  K     VTI G+E VP N+E  L  AVA+QPVSVAIDAG  DF
Sbjct: 213 TEDSYPYEGIDGKCDASKDNGVLVTIDGHEDVPENDENALLKAVANQPVSVAIDAGSSDF 272

Query: 276 QFYSSGIFSGSCGKQLNHGVAIVGYGEVGDNTYWLVKNSWGTEWGESGYIRMKRDSIDKR 335
           QFYS G+F+GSCG +LNHGVA VGYG      YW+V+NSWG EWGE GYI+++R+  +  
Sbjct: 273 QFYSEGVFTGSCGTELNHGVAAVGYGSERGKKYWIVRNSWGAEWGEGGYIKIEREIDEPE 332

Query: 336 GACGIAMEASYPIK 339
           G CGIAMEASYPIK
Sbjct: 333 GRCGIAMEASYPIK 344

[Close]

BLAST of CmaCh20G005860.1 vs. TAIR10
Match: AT5G45890.1 (AT5G45890.1 senescence-associated gene 12)

HSP 1 Score: 334.0 bits (855), Expect = 1.1e-91
Identity = 166/313 (53.04%), Postives = 223/313 (71.25%), Query Frame = 1


Query: 37  LQDRYKKWMNKHSREYKSREEQERRFTVYQLNVQYIDNFNSL--NHSYTLAENSFADLTN 96
           +Q R+ +WM KH R Y   +E+  R+ V++ NV+ I++ NS+    ++ LA N FADLTN
Sbjct: 34  MQKRHIEWMTKHGRVYADVKEENNRYVVFKNNVERIEHLNSIPAGRTFKLAVNQFADLTN 93

Query: 97  DEFKITYLGYQ-TDCLSDTC------FRYDHVIS--LPNHVDWRMEDAVTPVKDQGQCGS 156
           DEF+  Y G++    LS         FRY +V S  LP  VDWR + AVTP+K+QG CG 
Sbjct: 94  DEFRSMYTGFKGVSALSSQSQTKMSPFRYQNVSSGALPVSVDWRKKGAVTPIKNQGSCGC 153

Query: 157 CWAFSAVAAVEGIHKIRTGKLESLSEQELVDCDITLGNQGCDGGFMNKAFEYIKRSG-LT 216
           CWAFSAVAA+EG  +I+ GKL SLSEQ+LVDCD    + GC+GG M+ AFE+IK +G LT
Sbjct: 154 CWAFSAVAAIEGATQIKKGKLISLSEQQLVDCDTN--DFGCEGGLMDTAFEHIKATGGLT 213

Query: 217 TEREYPYRGIEAFCNTQKVRYHSVTISGYEKVPMNNEKKLKAAVAHQPVSVAIDAGGYDF 276
           TE  YPY+G +A CN++K    + +I+GYE VP+N+E+ L  AVAHQPVSV I+ GG+DF
Sbjct: 214 TESNYPYKGEDATCNSKKTNPKATSITGYEDVPVNDEQALMKAVAHQPVSVGIEGGGFDF 273

Query: 277 QFYSSGIFSGSCGKQLNHGVAIVGYGE-VGDNTYWLVKNSWGTEWGESGYIRMKRDSIDK 336
           QFYSSG+F+G C   L+H V  +GYGE    + YW++KNSWGT+WGESGY+R+++D  DK
Sbjct: 274 QFYSSGVFTGECTTYLDHAVTAIGYGESTNGSKYWIIKNSWGTKWGESGYMRIQKDVKDK 333

[Close]

BLAST of CmaCh20G005860.1 vs. TAIR10
Match: AT4G35350.1 (AT4G35350.1 xylem cysteine peptidase 1)

HSP 1 Score: 327.0 bits (837), Expect = 1.4e-89
Identity = 168/308 (54.55%), Postives = 205/308 (66.56%), Query Frame = 1


Query: 37  LQDRYKKWMNKHSREYKSREEQERRFTVYQLNVQYIDNFNSLNHSYTLAENSFADLTNDE 96
           L + ++ WM++HS+ YKS EE+  RF V++ N+ +ID  N+  +SY L  N FADLT++E
Sbjct: 47  LLELFESWMSEHSKAYKSVEEKVHRFEVFRENLMHIDQRNNEINSYWLGLNEFADLTHEE 106

Query: 97  FKITYLGYQTDCLS-----DTCFRYDHVISLPNHVDWRMEDAVTPVKDQGQCGSCWAFSA 156
           FK  YLG      S        FRY  +  LP  VDWR + AV PVKDQGQCGSCWAFS 
Sbjct: 107 FKGRYLGLAKPQFSRKRQPSANFRYRDITDLPKSVDWRKKGAVAPVKDQGQCGSCWAFST 166

Query: 157 VAAVEGIHKIRTGKLESLSEQELVDCDITLGNQGCDGGFMNKAFEYI-KRSGLTTEREYP 216
           VAAVEGI++I TG L SLSEQEL+DCD T  N GC+GG M+ AF+YI    GL  E +YP
Sbjct: 167 VAAVEGINQITTGNLSSLSEQELIDCDTTF-NSGCNGGLMDYAFQYIISTGGLHKEDDYP 226

Query: 217 YRGIEAFCNTQKVRYHSVTISGYEKVPMNNEKKLKAAVAHQPVSVAIDAGGYDFQFYSSG 276
           Y   E  C  QK     VTISGYE VP N+++ L  A+AHQPVSVAI+A G DFQFY  G
Sbjct: 227 YLMEEGICQEQKEDVERVTISGYEDVPENDDESLVKALAHQPVSVAIEASGRDFQFYKGG 286

Query: 277 IFSGSCGKQLNHGVAIVGYGEVGDNTYWLVKNSWGTEWGESGYIRMKRDSIDKRGACGIA 336
           +F+G CG  L+HGVA VGYG    + Y +VKNSWG  WGE G+IRMKR++    G CGI 
Sbjct: 287 VFNGKCGTDLDHGVAAVGYGSSKGSDYVIVKNSWGPRWGEKGFIRMKRNTGKPEGLCGIN 346

Query: 337 MEASYPIK 339
             ASYP K
Sbjct: 347 KMASYPTK 353

[Close]

BLAST of CmaCh20G005860.1 vs. NCBI nr
Match: gi|659117224|ref|XP_008458487.1| (PREDICTED: ervatamin-B-like [Cucumis melo])

HSP 1 Score: 521.2 bits (1341), Expect = 1.4e-144
Identity = 251/346 (72.54%), Postives = 289/346 (83.53%), Query Frame = 1


Query: 1   MEAYKTIWNMGLTSLILWVICTPSMASMATDSPSNG-----LQDRYKKWMNKHSREYKSR 60
           MEAYK IW++ L SLILWV  TP+  SMA D PS       LQDRY+KWM+K+ R+YKSR
Sbjct: 1   MEAYKMIWSVSLISLILWVFWTPTRVSMAMDYPSGSSNSGELQDRYQKWMDKYGRQYKSR 60

Query: 61  EEQERRFTVYQLNVQYIDNFNSLNHSYTLAENSFADLTNDEFKITYLGYQTDCL--SDTC 120
           EE E+RFT+YQ NVQYIDNFNSLNHSYTLAEN+F DLTN+EF  TYLGY+T  L  +DT 
Sbjct: 61  EEWEQRFTIYQANVQYIDNFNSLNHSYTLAENNFTDLTNEEFMATYLGYETVSLPDTDTF 120

Query: 121 FRYDHVISLPNHVDWRMEDAVTPVKDQGQCGSCWAFSAVAAVEGIHKIRTGKLESLSEQE 180
           FRY ++++LP +VDWR E AVTP+K+QGQCGSCWAFSAVAAVEGI+KI+ GKL SLSEQE
Sbjct: 121 FRYGNMVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQE 180

Query: 181 LVDCDITLGNQGCDGGFMNKAFEYIKRSGLTTEREYPYRGIEAFCNTQKVRYHSVTISGY 240
           LVDCD+T GNQGC+GG+M KAFE+IK++GLTTE EYPY    + C+ QK +Y SV+ISGY
Sbjct: 181 LVDCDVTSGNQGCNGGYMYKAFEFIKKTGLTTELEYPYTATRSACDKQKEKYQSVSISGY 240

Query: 241 EKVPMNNEKKLKAAVAHQPVSVAIDAGGYDFQFYSSGIFSGSCGKQLNHGVAIVGYGEVG 300
           EKVP+N+EK L+AAVA QPVSVAIDAGG DFQFYS GIFSG+CGKQLNHGVAIVGYGE  
Sbjct: 241 EKVPVNDEKSLQAAVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGVAIVGYGEDS 300

Query: 301 DNTYWLVKNSWGTEWGESGYIRMKRDSIDKRGACGIAMEASYPIKD 340
           +  YWLVKNSWGT WGESGYIRM RDS DK+G CGIAM ASYPIKD
Sbjct: 301 NQAYWLVKNSWGTSWGESGYIRMMRDSTDKQGTCGIAMMASYPIKD 346

[Close]

BLAST of CmaCh20G005860.1 vs. NCBI nr
Match: gi|700206934|gb|KGN62053.1| (hypothetical protein Csa_2G292830 [Cucumis sativus])

HSP 1 Score: 515.0 bits (1325), Expect = 1.0e-142
Identity = 244/336 (72.62%), Postives = 286/336 (85.12%), Query Frame = 1


Query: 9   NMGLTSLILWVICTPSMASMATD-----SPSNGLQDRYKKWMNKHSREYKSREEQERRFT 68
           N+ L  LILWV  TP + SMA D     S S+ +QDRY+KWM+K+ R+YKSREE ERRFT
Sbjct: 5   NVSLIFLILWVFWTPRLVSMAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREEWERRFT 64

Query: 69  VYQLNVQYIDNFNSLNHSYTLAENSFADLTNDEFKITYLGYQTDCLSDTCFRYDHVISLP 128
           +YQ NVQYIDNFNS+NHS+TLAEN+FADLTN+EFK TYLGY+T  + DTCFRY ++++LP
Sbjct: 65  IYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYGNMVNLP 124

Query: 129 NHVDWRMEDAVTPVKDQGQCGSCWAFSAVAAVEGIHKIRTGKLESLSEQELVDCDITLGN 188
            +VDWR E AVTP+K+QGQCGSCWAFSAVAAVEGI+KI+ GKL SLSEQELVDCD+T GN
Sbjct: 125 TNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCDVTSGN 184

Query: 189 QGCDGGFMNKAFEYIKRSGLTTEREYPYRGIEAFCNTQKVRYHSVTISGYEKVPMNNEKK 248
           QGC+GG+M KAFE+IKR+GLTTE EYPY+G E+ CN QK +Y  V+ISGYEKVP+N+EK 
Sbjct: 185 QGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNEQKEKYQFVSISGYEKVPVNDEKS 244

Query: 249 LKAAVAHQPVSVAIDAGGYDFQFYSSGIFSGSCGKQLNHGVAIVGYGEVGDNTYWLVKNS 308
           LKAAVA+QPVSVAIDA G +FQFYS GIFSG+CG QLNHGVAIVGYGE  +  YWLVKNS
Sbjct: 245 LKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQAYWLVKNS 304

Query: 309 WGTEWGESGYIRMKRDSIDKRGACGIAMEASYPIKD 340
           WGT+WGESGYIRMKRDS D++G CGIAM ASYP KD
Sbjct: 305 WGTDWGESGYIRMKRDSTDRQGTCGIAMMASYPTKD 340

[Close]

BLAST of CmaCh20G005860.1 vs. NCBI nr
Match: gi|449460678|ref|XP_004148072.1| (PREDICTED: ervatamin-B-like [Cucumis sativus])

HSP 1 Score: 497.3 bits (1279), Expect = 2.2e-137
Identity = 231/308 (75.00%), Postives = 271/308 (87.99%), Query Frame = 1


Query: 32  SPSNGLQDRYKKWMNKHSREYKSREEQERRFTVYQLNVQYIDNFNSLNHSYTLAENSFAD 91
           S S+ +QDRY+KWM+K+ R+YKSREE ERRFT+YQ NVQYIDNFNS+NHS+TLAEN+FAD
Sbjct: 10  SCSSDIQDRYQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFAD 69

Query: 92  LTNDEFKITYLGYQTDCLSDTCFRYDHVISLPNHVDWRMEDAVTPVKDQGQCGSCWAFSA 151
           LTN+EFK TYLGY+T  + DTCFRY ++++LP +VDWR E AVTP+K+QGQCGSCWAFSA
Sbjct: 70  LTNEEFKATYLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSA 129

Query: 152 VAAVEGIHKIRTGKLESLSEQELVDCDITLGNQGCDGGFMNKAFEYIKRSGLTTEREYPY 211
           VAAVEGI+KI+ GKL SLSEQELVDCD+T GNQGC+GG+M KAFE+IKR+GLTTE EYPY
Sbjct: 130 VAAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPY 189

Query: 212 RGIEAFCNTQKVRYHSVTISGYEKVPMNNEKKLKAAVAHQPVSVAIDAGGYDFQFYSSGI 271
           +G E+ CN QK +Y  V+ISGYEKVP+N+EK LKAAVA+QPVSVAIDA G +FQFYS GI
Sbjct: 190 QGAESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGI 249

Query: 272 FSGSCGKQLNHGVAIVGYGEVGDNTYWLVKNSWGTEWGESGYIRMKRDSIDKRGACGIAM 331
           FSG+CG QLNHGVAIVGYGE  +  YWLVKNSWGT+WGESGYIRMKRDS D++G CGIAM
Sbjct: 250 FSGNCGNQLNHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDRQGTCGIAM 309

Query: 332 EASYPIKD 340
            ASYP KD
Sbjct: 310 MASYPTKD 317

[Close]

BLAST of CmaCh20G005860.1 vs. NCBI nr
Match: gi|645245974|ref|XP_008229136.1| (PREDICTED: zingipain-2 [Prunus mume])

HSP 1 Score: 428.3 bits (1100), Expect = 1.2e-116
Identity = 198/342 (57.89%), Postives = 251/342 (73.39%), Query Frame = 1


Query: 1   MEAYKTIWNMGLTSLILWVICTPSMASMATDSP----SNGLQDRYKKWMNKHSREYKSRE 60
           ME    +    LT  ++W+ C  S A   T  P       +++RY++W+ K+ R YK+RE
Sbjct: 1   METSMVLTRASLTFFMVWIFCISSTACSETYKPLRTDPKAMKERYERWLQKYGRIYKNRE 60

Query: 61  EQERRFTVYQLNVQYIDNFNSLNHSYTLAENSFADLTNDEFKITYLGYQTDCLSDTCFRY 120
           E E RF VY+ N++++D  NS N SY L +N FAD+TN EF  T++G+QT     T F Y
Sbjct: 61  EAEYRFGVYKSNIEFVDFVNSQNQSYKLTDNKFADITNLEFTNTFMGFQTRSHPKTKFSY 120

Query: 121 DHVISLPNHVDWRMEDAVTPVKDQGQCGSCWAFSAVAAVEGIHKIRTGKLESLSEQELVD 180
           D    LP  VDWR   AVTP+K+QGQCGSCWAFSAVAAVEGI++I+TGKL SLSEQELVD
Sbjct: 121 DKDEDLPTAVDWRKNGAVTPIKNQGQCGSCWAFSAVAAVEGINQIKTGKLVSLSEQELVD 180

Query: 181 CDITLGNQGCDGGFMNKAFEYIKRSGLTTEREYPYRGIEAFCNTQKVRYHSVTISGYEKV 240
           CD+  GN+GC+GG+M KAF +IK +GL+TE++YPY+G +  C+   ++ H+V ISGYE +
Sbjct: 181 CDVKTGNEGCNGGYMEKAFSFIKDNGLSTEKDYPYKGSDGICDEDSLKNHAVNISGYESI 240

Query: 241 PMNNEKKLKAAVAHQPVSVAIDAGGYDFQFYSSGIFSGSCGKQLNHGVAIVGYGEVGDNT 300
           P N+EK L+AAVAHQPVSVA+DA  Y FQFYSSGIF+G CGK LNHGV  VGYGE     
Sbjct: 241 PANSEKSLQAAVAHQPVSVAVDAASYAFQFYSSGIFTGQCGKNLNHGVTAVGYGEDSGKK 300

Query: 301 YWLVKNSWGTEWGESGYIRMKRDSIDKRGACGIAMEASYPIK 339
           YW+VKNSWG +WGESGYIRM RDS+DK+G CGIAM+ASYP+K
Sbjct: 301 YWIVKNSWGPDWGESGYIRMTRDSVDKKGTCGIAMQASYPVK 342

[Close]

BLAST of CmaCh20G005860.1 vs. NCBI nr
Match: gi|595908837|ref|XP_007214244.1| (hypothetical protein PRUPE_ppa023515mg [Prunus persica])

HSP 1 Score: 423.3 bits (1087), Expect = 3.9e-115
Identity = 197/342 (57.60%), Postives = 250/342 (73.10%), Query Frame = 1


Query: 1   MEAYKTIWNMGLTSLILWVICTPSMASMATDSP----SNGLQDRYKKWMNKHSREYKSRE 60
           ME    +    LT L++W+ C  S A   T  P       +++RY++W+ K+ R YK+RE
Sbjct: 1   METSMVLTRASLTFLMVWIFCISSTACSETYKPLRTDPKAMKERYERWLQKYGRIYKNRE 60

Query: 61  EQERRFTVYQLNVQYIDNFNSLNHSYTLAENSFADLTNDEFKITYLGYQTDCLSDTCFRY 120
           E   RF VY+ N++++D  NS N SY L +N FAD+TN EF  T++G+QT     T F Y
Sbjct: 61  EAAYRFGVYKSNIEFVDFVNSQNLSYKLTDNKFADITNLEFTKTFMGFQTRSHPKTKFSY 120

Query: 121 DHVISLPNHVDWRMEDAVTPVKDQGQCGSCWAFSAVAAVEGIHKIRTGKLESLSEQELVD 180
           D    LP  VDWR   AVTP+K+QGQCGSCWAFSAVAAVEGI++I+TGKL SLSEQELVD
Sbjct: 121 DKDEELPTAVDWRKHGAVTPIKNQGQCGSCWAFSAVAAVEGINQIKTGKLVSLSEQELVD 180

Query: 181 CDITLGNQGCDGGFMNKAFEYIKRSGLTTEREYPYRGIEAFCNTQKVRYHSVTISGYEKV 240
           CD+  GN+GC+GG+M KAF +IK +GL+TE++YPY+G +  C+   ++  +V ISGYE +
Sbjct: 181 CDVKTGNEGCNGGYMEKAFSFIKDNGLSTEKDYPYKGSDGICDEDSLKNSAVNISGYESI 240

Query: 241 PMNNEKKLKAAVAHQPVSVAIDAGGYDFQFYSSGIFSGSCGKQLNHGVAIVGYGEVGDNT 300
           P N+EK L+AAVAHQPVSVA+DA GY FQFYSSG F+G CGK LNHGV  VGYGE     
Sbjct: 241 PANSEKSLQAAVAHQPVSVAVDAAGYAFQFYSSGTFTGQCGKNLNHGVTAVGYGEDSGKK 300

Query: 301 YWLVKNSWGTEWGESGYIRMKRDSIDKRGACGIAMEASYPIK 339
           YW+VKNSWG +WGESGYIRM RDS+DK+G CGIAM+ASYP+K
Sbjct: 301 YWIVKNSWGPDWGESGYIRMTRDSVDKKGTCGIAMQASYPVK 342

The following BLAST results are available for this feature:

BLAST of CmaCh20G005860.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CEP1_ARATH	1.2e-92	55.87	KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana GN=CEP1 PE=1 SV=...	[more]
CYSEP_RICCO	8.0e-92	55.84	Vignain OS=Ricinus communis GN=CYSEP PE=1 SV=1	[more]
CYSEP_VIGMU	1.4e-91	51.58	Vignain OS=Vigna mungo PE=1 SV=1	[more]
CEP2_ARATH	2.3e-91	55.73	KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana GN=CEP2 PE=1 SV=...	[more]
CYSEP_PHAVU	3.0e-91	50.87	Vignain OS=Phaseolus vulgaris PE=2 SV=2	[more]

BLAST of CmaCh20G005860.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LJV6_CUCSA	6.9e-143	72.62	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G292830 PE=3 SV=1	[more]
M5X1M2_PRUPE	2.8e-115	57.60	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023515mg PE=3 SV=1	[more]
G7ZUL7_MEDTR	5.4e-111	58.75	Cysteine proteinase OS=Medicago truncatula GN=MTR_2g090890 PE=3 SV=1	[more]
W9RAD3_9ROSA	1.6e-110	56.36	KDEL-tailed cysteine endopeptidase CEP2 OS=Morus notabilis GN=L484_001450 PE=3 S...	[more]
A0A151TPE0_CAJCA	2.5e-108	62.50	Vignain OS=Cajanus cajan GN=KK1_022504 PE=3 SV=1	[more]

BLAST of CmaCh20G005860.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G06260.1	3.3e-96	51.92	Cysteine proteinases superfamily protein	[more]
AT5G50260.1	7.0e-94	55.87	Cysteine proteinases superfamily protein	[more]
AT3G48340.1	1.3e-92	55.73	Cysteine proteinases superfamily protein	[more]
AT5G45890.1	1.1e-91	53.04	senescence-associated gene 12	[more]
AT4G35350.1	1.4e-89	54.55	xylem cysteine peptidase 1	[more]

BLAST of CmaCh20G005860.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659117224\|ref\|XP_008458487.1\|	1.4e-144	72.54	PREDICTED: ervatamin-B-like [Cucumis melo]	[more]
gi\|700206934\|gb\|KGN62053.1\|	1.0e-142	72.62	hypothetical protein Csa_2G292830 [Cucumis sativus]	[more]
gi\|449460678\|ref\|XP_004148072.1\|	2.2e-137	75.00	PREDICTED: ervatamin-B-like [Cucumis sativus]	[more]
gi\|645245974\|ref\|XP_008229136.1\|	1.2e-116	57.89	PREDICTED: zingipain-2 [Prunus mume]	[more]
gi\|595908837\|ref\|XP_007214244.1\|	3.9e-115	57.60	hypothetical protein PRUPE_ppa023515mg [Prunus persica]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR000169	Pept_cys_AS
IPR000668	Peptidase_C1A_C
IPR013128	Peptidase_C1A
IPR013201	Prot_inhib_I29
IPR025660	Pept_his_AS
IPR025661	Pept_asp_AS

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

Vocabulary: **Molecular Function**
Term	Definition
GO:0008234	cysteine-type peptidase activity

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0008234	cysteine-type peptidase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh20G005860	CmaCh20G005860	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh20G005860.1	CmaCh20G005860.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh20G005860.1.CDS.2	CmaCh20G005860.1.CDS.2	CDS
CmaCh20G005860.1.CDS.1	CmaCh20G005860.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh20G005860.1.exon.2	CmaCh20G005860.1.exon.2	exon
CmaCh20G005860.1.exon.1	CmaCh20G005860.1.exon.1	exon

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000169	Cysteine peptidase, cysteine active site	PROSITE	PS00139	THIOL_PROTEASE_CYS	coord: 140..151 scor
IPR000668	Peptidase C1A, papain C-terminal	PRINTS	PR00705	PAPAIN	coord: 282..292 score: 2.2E-10coord: 297..303 score: 2.2E-10coord: 140..155 score: 2.2
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 122..337 score: 2.9
IPR000668	Peptidase C1A, papain C-terminal	SMART	SM00645	pept_c1	coord: 122..337 score: 6.5E
IPR013128	Peptidase C1A	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 7..339 score: 3.1E
IPR013201	Cathepsin propeptide inhibitor domain (I29)	PFAM	PF08246	Inhibitor_I29	coord: 41..97 score: 7.0
IPR013201	Cathepsin propeptide inhibitor domain (I29)	SMART	SM00848	Inhibitor_I29_2	coord: 41..97 score: 2.1
IPR025660	Cysteine peptidase, histidine active site	PROSITE	PS00639	THIOL_PROTEASE_HIS	coord: 280..290 scor
IPR025661	Cysteine peptidase, asparagine active site	PROSITE	PS00640	THIOL_PROTEASE_ASN	coord: 297..316 scor
None	No IPR available	GENE3D	G3DSA:3.90.70.10		coord: 25..337 score: 1.1E
None	No IPR available	PANTHER	PTHR12411:SF333	CYSTEINE PROTEASE-LIKE PROTEIN-RELATED	coord: 7..339 score: 3.1E
None	No IPR available	unknown	SSF54001	Cysteine proteinases	coord: 38..337 score: 3.19E