CmaCh18G012080.1 (mRNA) Cucurbita maxima (Rimu)

BLAST of CmaCh18G012080.1 vs. Swiss-Prot
Match: GAOX2_ARATH (Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana GN=GA20OX2 PE=2 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 4.8e-117
Identity = 190/338 (56.21%), Postives = 247/338 (73.08%), Query Frame = 1

Query: 30  ESKVPESFVWPDDLKGSQEVPVLNVPHIDLNKFLSGNESDVEEVTRLVDEACRKHGFFVV 89
           +S++P  F+WPD+ K S ++P LNVP IDL+     ++    E  R++ EAC KHGFF+V
Sbjct: 39  QSQIPNQFIWPDEEKPSIDIPELNVPFIDLS-----SQDSTLEAPRVIAEACTKHGFFLV 98

Query: 90  VNHGVDMKVMESLHECMSEFFSMPMDVKQRAHRKFGENFGYAYSFFVRFSSNLPWKETLS 149
           VNHGV   ++   H  M  FF MP+  KQ+A RK GE+ GYA SF  RFS+ LPWKETLS
Sbjct: 99  VNHGVSESLIADAHRLMESFFDMPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLS 158

Query: 150 LPCVADGENSSSAYDYLIEKLGPTFSHHGKVYQDCAKAVNDLGMKILEVLGLSLGVSREY 209
                D   S +  DY  + LG  F   GKVYQD  +A++ L +KI+E+LGLSLGV+R+Y
Sbjct: 159 FQFSNDNSGSRTVQDYFSDTLGQEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRDY 218

Query: 210 FKNFYEDNDSIMRLNYYPPCAKPELVLGTGPHCDPTSITILHQDKVSGFQVCVDDQWYSI 269
           F+ F+E+NDSIMRLN+YPPC  P+L LGTGPHCDP+S+TILHQD V+G QV VD+QW SI
Sbjct: 219 FRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNGLQVFVDNQWQSI 278

Query: 270 PPTPDSFVINIGDTFKSLTNGIYKSCVHRAVVNSENVRKTMAFFICPNHDKVVRAPEELV 329
            P P +FV+NIGDTF +L+NGI+KSC+HRAVVN E+ RK+MAFF+CP  DKVV+ P +++
Sbjct: 279 RPNPKAFVVNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAFFLCPKKDKVVKPPSDIL 338

Query: 330 EKNPPRKYPDYTWGTLLEITQKHYRPDCNTLEALTDWI 368
           EK   RKYPD+TW   LE TQKHYR D NTL++ ++W+
Sbjct: 339 EKMKTRKYPDFTWSMFLEFTQKHYRADVNTLDSFSNWV 371

BLAST of CmaCh18G012080.1 vs. Swiss-Prot
Match: GAOX1_ARATH (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 421.8 bits (1083), Expect = 8.1e-117
Identity = 194/340 (57.06%), Postives = 246/340 (72.35%), Query Frame = 1

Query: 30  ESKVPESFVWPDDLKGSQEVPVLNVPHIDLNKFLSGNESDVEEVTRLVDEACRKHGFFVV 89
           ++ +P  F+WPDD K S  V  L+VP IDL   LS   S ++  +RL+ EAC+KHGFF+V
Sbjct: 37  QANIPNQFIWPDDEKPSINVLELDVPLIDLQNLLSDPSSTLD-ASRLISEACKKHGFFLV 96

Query: 90  VNHGVDMKVMESLHECMSEFFSMPMDVKQRAHRKFGENFGYAYSFFVRFSSNLPWKETLS 149
           VNHG+  +++   HE  S FF MP+  KQR  RK GE+ GYA SF  RFS+ LPWKETLS
Sbjct: 97  VNHGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLS 156

Query: 150 LPCVADGENSSSAYDYLIEKLGPTFSHHGKVYQDCAKAVNDLGMKILEVLGLSLGVSREY 209
                D   S S  DY  + LG  F   GKVYQ+  +A++ L +KI+E+LGLSLGV R+Y
Sbjct: 157 FRFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDY 216

Query: 210 FKNFYEDNDSIMRLNYYPPCAKPELVLGTGPHCDPTSITILHQDKVSGFQVCVDDQWYSI 269
           F+ F+E+NDSIMRLNYYPPC KP+L LGTGPHCDPTS+TILHQD V+G QV V++QW SI
Sbjct: 217 FREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSI 276

Query: 270 PPTPDSFVINIGDTFKSLTNGIYKSCVHRAVVNSENVRKTMAFFICPNHDKVVRAPEELV 329
            P P +FV+NIGDTF +L+N  YKSC+HRAVVNSE+ RK++AFF+CP  D+VV  P EL+
Sbjct: 277 RPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELL 336

Query: 330 EKNPPRKYPDYTWGTLLEITQKHYRPDCNTLEALTDWIQK 370
           +    R+YPD+TW   LE TQKHYR D NTL+A +DW+ K
Sbjct: 337 DSITSRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDWLTK 375

BLAST of CmaCh18G012080.1 vs. Swiss-Prot
Match: GAOX3_ARATH (Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana GN=GA20OX3 PE=2 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 8.4e-114
Identity = 201/375 (53.60%), Postives = 255/375 (68.00%), Query Frame = 1

Query: 1   MATESLSSAHSDLNQETKGEYRGTF----AGPDESKVPESFVWPDDLKGSQEVPVLNVPH 60
           MATE +++     ++    E    F           +P+ FVWPD  K S +V  L VP 
Sbjct: 1   MATECIATVPQIFSENKTKEDSSIFDAKLLNQHSHHIPQQFVWPDHEKPSTDVQPLQVPL 60

Query: 61  IDLNKFLSGNESDVEEVTRLVDEACRKHGFFVVVNHGVDMKVMESLHECMSEFFSMPMDV 120
           IDL  FLSG+     E TRLV +A  KHGFF++ NHGVD  ++   +  M  FF  P   
Sbjct: 61  IDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNHGVDESLLSRAYLHMDSFFKAPACE 120

Query: 121 KQRAHRKFGENFGYAYSFFVRFSSNLPWKETLSLPCVADGE-NSSSAYDYLIEKLGPTFS 180
           KQ+A RK+GE+ GYA SF  RFSS LPWKETLS     + + +S +  D++ +K+G  + 
Sbjct: 121 KQKAQRKWGESSGYASSFVGRFSSKLPWKETLSFKFSPEEKIHSQTVKDFVSKKMGDGYE 180

Query: 181 HHGKVYQDCAKAVNDLGMKILEVLGLSLGVSREYFKNFYEDNDSIMRLNYYPPCAKPELV 240
             GKVYQ+ A+A+N L +KI+E+LG+SLGV R YFK F+ED+DSI RLNYYP C +PEL 
Sbjct: 181 DFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFKEFFEDSDSIFRLNYYPQCKQPELA 240

Query: 241 LGTGPHCDPTSITILHQDKVSGFQVCVDDQWYSIPPTPDSFVINIGDTFKSLTNGIYKSC 300
           LGTGPHCDPTS+TILHQD+V G QV VD++W SIPP P +FV+NIGDTF +LTNG YKSC
Sbjct: 241 LGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPPNPHAFVVNIGDTFMALTNGRYKSC 300

Query: 301 VHRAVVNSENVRKTMAFFICPNHDKVVRAPEELVE--KNPPRKYPDYTWGTLLEITQKHY 360
           +HRAVVNSE  RKT AFF+CP  +KVV+ PEELV   K+  RKYPD+TW   LE TQKHY
Sbjct: 301 LHRAVVNSERERKTFAFFLCPKGEKVVKPPEELVNGVKSGERKYPDFTWSMFLEFTQKHY 360

Query: 361 RPDCNTLEALTDWIQ 369
           R D NTL+  + W++
Sbjct: 361 RADMNTLDEFSIWLK 375

BLAST of CmaCh18G012080.1 vs. Swiss-Prot
Match: GAOX4_ARATH (Gibberellin 20 oxidase 4 OS=Arabidopsis thaliana GN=GA20OX4 PE=2 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 2.3e-111
Identity = 188/339 (55.46%), Postives = 238/339 (70.21%), Query Frame = 1

Query: 33  VPESFVWPDDLKGSQEVPVLNVPHIDLNKFLSGNESDVEEVTRLVDEACRKHGFFVVVNH 92
           +P+ FVWPD  K S+ VP+L VP IDL  FLS +   V E  RLV EA +KHGFF+V NH
Sbjct: 36  IPQEFVWPDHEKPSKNVPILQVPVIDLAGFLSNDPLLVSEAERLVSEAAKKHGFFLVTNH 95

Query: 93  GVDMKVMESLHECMSEFFSMPMDVKQRAHRKFGENFGYAYSFFVRFSSNLPWKETLSL-- 152
           GVD +++ + H+ M  FF  P   K +A RK GE  GYA SF  RF  NLPWKETLS   
Sbjct: 96  GVDERLLSTAHKLMDTFFKSPNYEKLKAQRKVGETTGYASSFVGRFKENLPWKETLSFSF 155

Query: 153 -PCVADGENSSSAYDYLIEKLGPTFSHHGKVYQDCAKAVNDLGMKILEVLGLSLGVSREY 212
            P       S +  +Y+ + +G  +   G VYQ+ A+ +++L +KI+E+LG+SLG+ RE+
Sbjct: 156 SPTEKSENYSQTVKNYISKTMGDGYKDFGSVYQEYAETMSNLSLKIMELLGMSLGIKREH 215

Query: 213 FKNFYEDNDSIMRLNYYPPCAKPELVLGTGPHCDPTSITILHQDKVSGFQVCVDDQWYSI 272
           F+ F+EDN+SI RLNYYP C +P+LVLGTGPHCDPTS+TIL QD+VSG QV VD+QW SI
Sbjct: 216 FREFFEDNESIFRLNYYPKCKQPDLVLGTGPHCDPTSLTILQQDQVSGLQVFVDNQWQSI 275

Query: 273 PPTPDSFVINIGDTFKSLTNGIYKSCVHRAVVNSENVRKTMAFFICPNHDKVVRAPEELV 332
           PP P + V+NIGDT  +LTNGIYKSC+HRAVVN E  RKT+AFF+CP  DKVV+ P EL 
Sbjct: 276 PPIPQALVVNIGDTLMALTNGIYKSCLHRAVVNGETTRKTLAFFLCPKVDKVVKPPSELE 335

Query: 333 EKNPPRKYPDYTWGTLLEITQKHYRPDCNTLEALTDWIQ 369
            +   R YPD+TW   LE T KHYR D NTLE  T+W++
Sbjct: 336 GE---RAYPDFTWSMFLEFTMKHYRADMNTLEEFTNWLK 371

BLAST of CmaCh18G012080.1 vs. Swiss-Prot
Match: GAO1A_WHEAT (Gibberellin 20 oxidase 1-A OS=Triticum aestivum GN=GA20ox1A PE=2 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 3.0e-103
Identity = 179/339 (52.80%), Postives = 232/339 (68.44%), Query Frame = 1

Query: 31  SKVPESFVWPDDLKGSQEVPV-LNVPHIDLNKFLSGNESDVEEVTRLVDEACRKHGFFVV 90
           S +P  F+WP+    + +    L+VP I++   LSG+ +   EVTRLV EAC +HGFF V
Sbjct: 15  SDIPSQFIWPEGESPTPDAAEELHVPLINIGGMLSGDAAAAAEVTRLVGEACERHGFFQV 74

Query: 91  VNHGVDMKVMESLHECMSEFFSMPMDVKQRAHRKFGENFGYAYSFFVRFSSNLPWKETLS 150
           VNHG+D +++   H C+  FF+MP+  KQRA R+ GE+ GYA SF  RF+S LPWKETLS
Sbjct: 75  VNHGIDAELLADAHRCVDNFFTMPLPEKQRALRRPGESCGYASSFTGRFASKLPWKETLS 134

Query: 151 L-PCVADGENSSSAYDYLIEKLGPTFSHHGKVYQDCAKAVNDLGMKILEVLGLSLGVSRE 210
              C +D    +   DY++  LG      G+VY      ++ L ++I+EVLG SLGV R 
Sbjct: 135 FRSCPSD---PALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVGRA 194

Query: 211 YFKNFYEDNDSIMRLNYYPPCAKPELVLGTGPHCDPTSITILHQDKVSGFQVCVDDQWYS 270
           +++ F+E NDSIMRLNYYPPC +P   LGTGPHCDPTS+TILHQD V G QV  + +W S
Sbjct: 195 HYRRFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRWRS 254

Query: 271 IPPTPDSFVINIGDTFKSLTNGIYKSCVHRAVVNSENVRKTMAFFICPNHDKVVRAPEEL 330
           I P  D+FV+NIGDTF +L+NG YKSC+HRAVVNS   RK++AFF+CP  DKVV  P  L
Sbjct: 255 IRPRADAFVVNIGDTFMALSNGRYKSCLHRAVVNSRVPRKSLAFFLCPEMDKVVAPPGTL 314

Query: 331 VEKNPPRKYPDYTWGTLLEITQKHYRPDCNTLEALTDWI 368
           V+ + PR YPD+TW +LL+ TQKHYR D  TLE  + WI
Sbjct: 315 VDASNPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSWI 350

BLAST of CmaCh18G012080.1 vs. TrEMBL
Match: Q39541_CUCMA (Gibberellin 20-oxidase OS=Cucurbita maxima PE=2 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 6.0e-167
Identity = 274/384 (71.35%), Postives = 327/384 (85.16%), Query Frame = 1

Query: 1   MATESLSSAHSDLNQETKGEYRGTFAGPDESKVPESFVWPDDLKGSQEVPVLNVPHIDLN 60
           +ATES   A S+LN+E KGEYR  F G DESKVPE F+W +  + S+ +PVL+VP IDL 
Sbjct: 7   VATES---APSNLNEEMKGEYRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVPTIDLE 66

Query: 61  KFLSGNESDVEEVTRLVDEACRKHGFFVVVNHGVDMKVMESLHECMSEFFSMPMDVKQRA 120
           KF+SG++S VEE TRLVDEACR+HG F VVNHGVD+++M  +H+CM+EFF+MP+DVKQRA
Sbjct: 67  KFMSGDKSYVEEATRLVDEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLDVKQRA 126

Query: 121 HRKFGENFGYAYSFFVRFSSNLPWKETLSLPCVADGENSSSAYDYLIEKLGPTFSHHGKV 180
            RK GE++GY  SFF RF+SNLPWKET SL CVA  +NSS+A+DY+++ LGP+FSHHGK 
Sbjct: 127 KRKVGESYGYTNSFFGRFASNLPWKETFSLRCVA-AQNSSAAHDYVLDTLGPSFSHHGKA 186

Query: 181 YQDCAKAVNDLGMKILEVLGLSLGVSREYFKNFYEDNDSIMRLNYYPPCAKPELVLGTGP 240
           YQ+C  A+N+LG KI+E+LGLSLG+SREYFKNF+EDNDSI+RLNYYP C KPE+VLGTGP
Sbjct: 187 YQECGIALNELGTKIVELLGLSLGISREYFKNFFEDNDSILRLNYYPTCDKPEVVLGTGP 246

Query: 241 HCDPTSITILHQDKVSGFQVCVDDQWYSIPPTPDSFVINIGDTFKSLTNGIYKSCVHRAV 300
           H DPTS+TILHQD VSG QVC +DQWYSIPP P++FVINIGDTF SLTNGIYK C+HRAV
Sbjct: 247 HTDPTSVTILHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHRAV 306

Query: 301 VNSENVRKTMAFFICPNHDKVVRAPEELVEKNPPRKYPDYTWGTLLEITQKHYRPDCNTL 360
           VNS N RK++AFF+CP+HDKVVRAPEELVEK+PPRKYPDY W  LLE+TQK YRPDCNTL
Sbjct: 307 VNSMNARKSLAFFLCPSHDKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRPDCNTL 366

Query: 361 EALTDWIQKGKPLDNGSTITAPSA 385
           EA   W+Q+GK LD GSTITAPSA
Sbjct: 367 EAFKTWVQEGKALDTGSTITAPSA 386

BLAST of CmaCh18G012080.1 vs. TrEMBL
Match: O23762_CUCMA (Gibberellin 20-oxidase OS=Cucurbita maxima PE=2 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 4.3e-165
Identity = 272/384 (70.83%), Postives = 325/384 (84.64%), Query Frame = 1

Query: 1   MATESLSSAHSDLNQETKGEYRGTFAGPDESKVPESFVWPDDLKGSQEVPVLNVPHIDLN 60
           +ATES   A S+LN+E KGEYR  F G DESKVPE F+W +  + S+ +PVL+VP IDL 
Sbjct: 7   VATES---APSNLNEEMKGEYRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVPTIDLE 66

Query: 61  KFLSGNESDVEEVTRLVDEACRKHGFFVVVNHGVDMKVMESLHECMSEFFSMPMDVKQRA 120
           KF+SG++S VEE TRLVDEACR+HG F VVNHGVD+++M  +H+CM+EFF+MP+DVKQRA
Sbjct: 67  KFMSGDKSYVEEATRLVDEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLDVKQRA 126

Query: 121 HRKFGENFGYAYSFFVRFSSNLPWKETLSLPCVADGENSSSAYDYLIEKLGPTFSHHGKV 180
            RK GE++GY  SFF RF+SNLPWKET SL CVA  +NSS+A+DY+++ LG +FSHHGK 
Sbjct: 127 KRKVGESYGYTNSFFGRFASNLPWKETFSLRCVA-AQNSSAAHDYVLDTLGSSFSHHGKA 186

Query: 181 YQDCAKAVNDLGMKILEVLGLSLGVSREYFKNFYEDNDSIMRLNYYPPCAKPELVLGTGP 240
           YQ+C  A+N+LG KI+E+LGLSLG+SREYFKNFY+DNDSI+RLNYYP C KPE+VLGTGP
Sbjct: 187 YQECGIALNELGTKIVELLGLSLGISREYFKNFYKDNDSILRLNYYPTCDKPEVVLGTGP 246

Query: 241 HCDPTSITILHQDKVSGFQVCVDDQWYSIPPTPDSFVINIGDTFKSLTNGIYKSCVHRAV 300
           H DPTS+TILHQD VSG QVC +DQWYSIPP P++FVINIGDTF SLTNGIYK C+HRAV
Sbjct: 247 HTDPTSVTILHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHRAV 306

Query: 301 VNSENVRKTMAFFICPNHDKVVRAPEELVEKNPPRKYPDYTWGTLLEITQKHYRPDCNTL 360
           VNS N RK++AFF+CP+HDKVVRAPEELVEK+PPRKYPDY W  LLE+TQK YRP CNTL
Sbjct: 307 VNSMNARKSLAFFLCPSHDKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRPACNTL 366

Query: 361 EALTDWIQKGKPLDNGSTITAPSA 385
           EA   W+Q+GK LD GSTITAPSA
Sbjct: 367 EAFKTWVQEGKALDTGSTITAPSA 386

BLAST of CmaCh18G012080.1 vs. TrEMBL
Match: Q96498_9ROSI (Gibberellin 20-oxidase OS=Marah macrocarpus PE=2 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 5.4e-152
Identity = 251/369 (68.02%), Postives = 299/369 (81.03%), Query Frame = 1

Query: 1   MATESLSSAHSDLNQETKGEYRGTFAGPDESKVPESFVWPDDLKGSQEVPVLNVPHIDLN 60
           MA   + +  S   +E K E++  F G  ES VPE+F+WPD+ K + + P L+VPHIDL 
Sbjct: 1   MAITCMMATESSPAEEVKREHQVAFGGAGESSVPENFIWPDEFKATADAPELHVPHIDLK 60

Query: 61  KFLSGNESDVEEVTRLVDEACRKHGFFVVVNHGVDMKVMESLHECMSEFFSMPMDVKQRA 120
           K LSG+E DVEE TRLVDEACRKHGFFVVVNHGVD ++M  +HECM+EFF++P+DVKQ+A
Sbjct: 61  KVLSGDEKDVEEATRLVDEACRKHGFFVVVNHGVDKELMNKVHECMNEFFTLPLDVKQKA 120

Query: 121 HRKFGENFGYAYSFFVRFSSNLPWKETLSLPCVADGENSSSAYDYLIEKLGPTFSHHGKV 180
           HRK GENFGYA SF  RFS+ LPWKET SL  +A  ENSS+A DY+ + LGP FSHHG+V
Sbjct: 121 HRKVGENFGYANSFIGRFSTKLPWKETFSLRYLAH-ENSSTARDYVSQVLGPEFSHHGEV 180

Query: 181 YQDCAKAVNDLGMKILEVLGLSLGVSREYFKNFYEDNDSIMRLNYYPPCAKPELVLGTGP 240
           YQ+C KA++DL ++I+E+LGLSLG+SRE F+ FYEDNDSIMR+NYYP C KPEL LGTGP
Sbjct: 181 YQECGKALSDLSLRIVELLGLSLGISRETFRKFYEDNDSIMRMNYYPRCEKPELTLGTGP 240

Query: 241 HCDPTSITILHQDKVSGFQVCVDDQWYSIPPTPDSFVINIGDTFKSLTNGIYKSCVHRAV 300
           HCDPTSITILHQD VSG QV VDDQW+SIPPT DSFV+N+GDTF SLTNG+YKSC HRAV
Sbjct: 241 HCDPTSITILHQDDVSGLQVYVDDQWHSIPPTEDSFVVNVGDTFMSLTNGVYKSCFHRAV 300

Query: 301 VNSENVRKTMAFFICPNHDKVVRAPEELVEKNPPRKYPDYTWGTLLEITQKHYRPDCNTL 360
           VN +  RK+MAFF+CP  +KVVRAPEELVEK PPRK+PDYTW  LLE+TQK+YR D NT 
Sbjct: 301 VNCKEARKSMAFFLCPAVEKVVRAPEELVEKYPPRKFPDYTWPMLLEMTQKYYRADSNTF 360

Query: 361 EALTDWIQK 370
            A T WIQ+
Sbjct: 361 NAFTTWIQQ 368

BLAST of CmaCh18G012080.1 vs. TrEMBL
Match: Q9XHR0_CITLA (Gibberellin 20-oxidase OS=Citrullus lanatus GN=WGA20OXI PE=2 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 3.9e-150
Identity = 252/383 (65.80%), Postives = 307/383 (80.16%), Query Frame = 1

Query: 1   MATESLSSAHSDLNQETKGEYRGTFAGPDESKVPESFVWPDDLKGSQEVPVLNVPHIDLN 60
           MA + + S       ++K  +R  F   +ES VPE+FVWPD+ K     P L+VPHIDL+
Sbjct: 1   MAMKCMMSTGESKEMKSK-LHRVEFGCSEESMVPENFVWPDEFKAKDGAPELHVPHIDLH 60

Query: 61  KFLSGNESDVEEVTRLVDEACRKHGFFVVVNHGVDMKVMESLHECMSEFFSMPMDVKQRA 120
           KFL+GNESD+EE TRLVDEACRKHGFFV+VNHGVDM++++ +HECM EFF++P DVKQ++
Sbjct: 61  KFLNGNESDIEEATRLVDEACRKHGFFVLVNHGVDMELIKGVHECMDEFFTLPFDVKQKS 120

Query: 121 HRKFGENFGYAYSFFVRFSSNLPWKETLSLPCVADGENSSSAYDYLIEKLGPTFSHHGKV 180
            RKFGEN+GYA SF  RFS+NLPWKETLSLP VA+  NS+S  D++ + +GP  SH GKV
Sbjct: 121 QRKFGENYGYANSFIGRFSNNLPWKETLSLPYVAN-HNSTSVQDFVSKCVGPELSHQGKV 180

Query: 181 YQDCAKAVNDLGMKILEVLGLSLGVSREYFKNFYEDNDSIMRLNYYPPCAKPELVLGTGP 240
           YQ+C KA++DL +KI+E+LGLSLGV +E FK FYEDNDSI+RLNYYPPC KPEL LGTGP
Sbjct: 181 YQECGKALSDLSLKIVELLGLSLGVPKEKFKKFYEDNDSIIRLNYYPPCEKPELTLGTGP 240

Query: 241 HCDPTSITILHQDKVSGFQVCVDDQWYSIPPTPDSFVINIGDTFKSLTNGIYKSCVHRAV 300
           HCDPTSITILHQD VSG QV VDD+W+SIPPT DSFVINIGDTF +LTNG+YKSC HRAV
Sbjct: 241 HCDPTSITILHQDHVSGLQVYVDDEWHSIPPTKDSFVINIGDTFMALTNGVYKSCFHRAV 300

Query: 301 VNSENVRKTMAFFICPNHDKVVRAPEELVEKNPPRKYPDYTWGTLLEITQKHYRPDCNTL 360
           VN + VRK+MAFF+CP  DKVVRAP+E+VEKNPPRK+PDYTW  LLE+TQK YR D NT 
Sbjct: 301 VNCKEVRKSMAFFLCPAADKVVRAPDEVVEKNPPRKFPDYTWPMLLELTQKFYRADSNTF 360

Query: 361 EALTDWIQKGKPLDNGSTITAPS 384
           +A   W++K K   + +TI  P+
Sbjct: 361 QAFIPWLEKQK---SANTIAPPA 378

BLAST of CmaCh18G012080.1 vs. TrEMBL
Match: I0J1I7_CUCMA (Gibberellin 20-oxidase OS=Cucurbita maxima GN=ga20ox4 PE=2 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 8.1e-148
Identity = 248/381 (65.09%), Postives = 297/381 (77.95%), Query Frame = 1

Query: 3   TESLSSAHSDLNQETKGEYRGTFAGPDESKVPESFVWPDDLKGSQEVPVLNVPHIDLNKF 62
           T + S   S L QE K ++R  F   DES VPE+F+WPD+ K  + VP L+VPHIDL KF
Sbjct: 7   TTAKSQGESKLKQEMKRKHRVEFGSSDESMVPENFIWPDEFKARESVPELDVPHIDLQKF 66

Query: 63  LSGNESDVEEVTRLVDEACRKHGFFVVVNHGVDMKVMESLHECMSEFFSMPMDVKQRAHR 122
           +S NE+D+EE TRLVDEACRKHGFFV+VNHGVDM++M+++HECM EFFS+P+DVKQ+A R
Sbjct: 67  VSENENDIEEATRLVDEACRKHGFFVLVNHGVDMELMKNVHECMDEFFSLPLDVKQKAQR 126

Query: 123 KFGENFGYAYSFFVRFSSNLPWKETLSLPCVADGENSSSAYDYLIEKLGPTFSHHGKVYQ 182
           K GEN+GYA SF  RFSS LPWKET SL   A   N    +DY+   LGP FS  GKVY+
Sbjct: 127 KVGENYGYANSFIGRFSSKLPWKETFSLRYAAH-HNLPVTHDYVCHHLGPEFSQQGKVYE 186

Query: 183 DCAKAVNDLGMKILEVLGLSLGVSREYFKNFYEDNDSIMRLNYYPPCAKPELVLGTGPHC 242
           +C +A++DL +KI+E+LGLSLGV +E FK F+EDNDSI+RLNYYPPC KPEL LGTGPHC
Sbjct: 187 ECGEALSDLSLKIVELLGLSLGVPKEKFKKFFEDNDSIIRLNYYPPCNKPELTLGTGPHC 246

Query: 243 DPTSITILHQDKVSGFQVCVDDQWYSIPPTPDSFVINIGDTFKSLTNGIYKSCVHRAVVN 302
           DPTSITILHQD VSG QV VDDQW+SIPP  DSFVINIGDTF +LTNG+YKSC HRAVVN
Sbjct: 247 DPTSITILHQDHVSGLQVYVDDQWHSIPPIKDSFVINIGDTFMALTNGVYKSCFHRAVVN 306

Query: 303 SENVRKTMAFFICPNHDKVVRAPEELVEKNPPRKYPDYTWGTLLEITQKHYRPDCNTLEA 362
               RK++ FF+CP  DKVVRAP+E+V+KNPPRK+PDYTW  LLE+TQK YR D NT +A
Sbjct: 307 CREARKSIVFFLCPADDKVVRAPDEIVDKNPPRKFPDYTWPMLLELTQKFYRSDSNTFKA 366

Query: 363 LTDWIQKGKPLDNGSTITAPS 384
            T W+Q  K L   ++  APS
Sbjct: 367 FTTWLQH-KNLATAASSLAPS 385

BLAST of CmaCh18G012080.1 vs. TAIR10
Match: AT5G51810.1 (AT5G51810.1 gibberellin 20 oxidase 2)

HSP 1 Score: 422.5 bits (1085), Expect = 2.7e-118
Identity = 190/338 (56.21%), Postives = 247/338 (73.08%), Query Frame = 1

Query: 30  ESKVPESFVWPDDLKGSQEVPVLNVPHIDLNKFLSGNESDVEEVTRLVDEACRKHGFFVV 89
           +S++P  F+WPD+ K S ++P LNVP IDL+     ++    E  R++ EAC KHGFF+V
Sbjct: 39  QSQIPNQFIWPDEEKPSIDIPELNVPFIDLS-----SQDSTLEAPRVIAEACTKHGFFLV 98

Query: 90  VNHGVDMKVMESLHECMSEFFSMPMDVKQRAHRKFGENFGYAYSFFVRFSSNLPWKETLS 149
           VNHGV   ++   H  M  FF MP+  KQ+A RK GE+ GYA SF  RFS+ LPWKETLS
Sbjct: 99  VNHGVSESLIADAHRLMESFFDMPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLS 158

Query: 150 LPCVADGENSSSAYDYLIEKLGPTFSHHGKVYQDCAKAVNDLGMKILEVLGLSLGVSREY 209
                D   S +  DY  + LG  F   GKVYQD  +A++ L +KI+E+LGLSLGV+R+Y
Sbjct: 159 FQFSNDNSGSRTVQDYFSDTLGQEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRDY 218

Query: 210 FKNFYEDNDSIMRLNYYPPCAKPELVLGTGPHCDPTSITILHQDKVSGFQVCVDDQWYSI 269
           F+ F+E+NDSIMRLN+YPPC  P+L LGTGPHCDP+S+TILHQD V+G QV VD+QW SI
Sbjct: 219 FRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNGLQVFVDNQWQSI 278

Query: 270 PPTPDSFVINIGDTFKSLTNGIYKSCVHRAVVNSENVRKTMAFFICPNHDKVVRAPEELV 329
            P P +FV+NIGDTF +L+NGI+KSC+HRAVVN E+ RK+MAFF+CP  DKVV+ P +++
Sbjct: 279 RPNPKAFVVNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAFFLCPKKDKVVKPPSDIL 338

Query: 330 EKNPPRKYPDYTWGTLLEITQKHYRPDCNTLEALTDWI 368
           EK   RKYPD+TW   LE TQKHYR D NTL++ ++W+
Sbjct: 339 EKMKTRKYPDFTWSMFLEFTQKHYRADVNTLDSFSNWV 371

BLAST of CmaCh18G012080.1 vs. TAIR10
Match: AT4G25420.1 (AT4G25420.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 421.8 bits (1083), Expect = 4.6e-118
Identity = 194/340 (57.06%), Postives = 246/340 (72.35%), Query Frame = 1

Query: 30  ESKVPESFVWPDDLKGSQEVPVLNVPHIDLNKFLSGNESDVEEVTRLVDEACRKHGFFVV 89
           ++ +P  F+WPDD K S  V  L+VP IDL   LS   S ++  +RL+ EAC+KHGFF+V
Sbjct: 37  QANIPNQFIWPDDEKPSINVLELDVPLIDLQNLLSDPSSTLD-ASRLISEACKKHGFFLV 96

Query: 90  VNHGVDMKVMESLHECMSEFFSMPMDVKQRAHRKFGENFGYAYSFFVRFSSNLPWKETLS 149
           VNHG+  +++   HE  S FF MP+  KQR  RK GE+ GYA SF  RFS+ LPWKETLS
Sbjct: 97  VNHGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLS 156

Query: 150 LPCVADGENSSSAYDYLIEKLGPTFSHHGKVYQDCAKAVNDLGMKILEVLGLSLGVSREY 209
                D   S S  DY  + LG  F   GKVYQ+  +A++ L +KI+E+LGLSLGV R+Y
Sbjct: 157 FRFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDY 216

Query: 210 FKNFYEDNDSIMRLNYYPPCAKPELVLGTGPHCDPTSITILHQDKVSGFQVCVDDQWYSI 269
           F+ F+E+NDSIMRLNYYPPC KP+L LGTGPHCDPTS+TILHQD V+G QV V++QW SI
Sbjct: 217 FREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSI 276

Query: 270 PPTPDSFVINIGDTFKSLTNGIYKSCVHRAVVNSENVRKTMAFFICPNHDKVVRAPEELV 329
            P P +FV+NIGDTF +L+N  YKSC+HRAVVNSE+ RK++AFF+CP  D+VV  P EL+
Sbjct: 277 RPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELL 336

Query: 330 EKNPPRKYPDYTWGTLLEITQKHYRPDCNTLEALTDWIQK 370
           +    R+YPD+TW   LE TQKHYR D NTL+A +DW+ K
Sbjct: 337 DSITSRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDWLTK 375

BLAST of CmaCh18G012080.1 vs. TAIR10
Match: AT5G07200.1 (AT5G07200.1 gibberellin 20-oxidase 3)

HSP 1 Score: 411.8 bits (1057), Expect = 4.7e-115
Identity = 201/375 (53.60%), Postives = 255/375 (68.00%), Query Frame = 1

Query: 1   MATESLSSAHSDLNQETKGEYRGTF----AGPDESKVPESFVWPDDLKGSQEVPVLNVPH 60
           MATE +++     ++    E    F           +P+ FVWPD  K S +V  L VP 
Sbjct: 1   MATECIATVPQIFSENKTKEDSSIFDAKLLNQHSHHIPQQFVWPDHEKPSTDVQPLQVPL 60

Query: 61  IDLNKFLSGNESDVEEVTRLVDEACRKHGFFVVVNHGVDMKVMESLHECMSEFFSMPMDV 120
           IDL  FLSG+     E TRLV +A  KHGFF++ NHGVD  ++   +  M  FF  P   
Sbjct: 61  IDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNHGVDESLLSRAYLHMDSFFKAPACE 120

Query: 121 KQRAHRKFGENFGYAYSFFVRFSSNLPWKETLSLPCVADGE-NSSSAYDYLIEKLGPTFS 180
           KQ+A RK+GE+ GYA SF  RFSS LPWKETLS     + + +S +  D++ +K+G  + 
Sbjct: 121 KQKAQRKWGESSGYASSFVGRFSSKLPWKETLSFKFSPEEKIHSQTVKDFVSKKMGDGYE 180

Query: 181 HHGKVYQDCAKAVNDLGMKILEVLGLSLGVSREYFKNFYEDNDSIMRLNYYPPCAKPELV 240
             GKVYQ+ A+A+N L +KI+E+LG+SLGV R YFK F+ED+DSI RLNYYP C +PEL 
Sbjct: 181 DFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFKEFFEDSDSIFRLNYYPQCKQPELA 240

Query: 241 LGTGPHCDPTSITILHQDKVSGFQVCVDDQWYSIPPTPDSFVINIGDTFKSLTNGIYKSC 300
           LGTGPHCDPTS+TILHQD+V G QV VD++W SIPP P +FV+NIGDTF +LTNG YKSC
Sbjct: 241 LGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPPNPHAFVVNIGDTFMALTNGRYKSC 300

Query: 301 VHRAVVNSENVRKTMAFFICPNHDKVVRAPEELVE--KNPPRKYPDYTWGTLLEITQKHY 360
           +HRAVVNSE  RKT AFF+CP  +KVV+ PEELV   K+  RKYPD+TW   LE TQKHY
Sbjct: 301 LHRAVVNSERERKTFAFFLCPKGEKVVKPPEELVNGVKSGERKYPDFTWSMFLEFTQKHY 360

Query: 361 RPDCNTLEALTDWIQ 369
           R D NTL+  + W++
Sbjct: 361 RADMNTLDEFSIWLK 375

BLAST of CmaCh18G012080.1 vs. TAIR10
Match: AT1G60980.1 (AT1G60980.1 gibberellin 20-oxidase 4)

HSP 1 Score: 403.7 bits (1036), Expect = 1.3e-112
Identity = 188/339 (55.46%), Postives = 238/339 (70.21%), Query Frame = 1

Query: 33  VPESFVWPDDLKGSQEVPVLNVPHIDLNKFLSGNESDVEEVTRLVDEACRKHGFFVVVNH 92
           +P+ FVWPD  K S+ VP+L VP IDL  FLS +   V E  RLV EA +KHGFF+V NH
Sbjct: 36  IPQEFVWPDHEKPSKNVPILQVPVIDLAGFLSNDPLLVSEAERLVSEAAKKHGFFLVTNH 95

Query: 93  GVDMKVMESLHECMSEFFSMPMDVKQRAHRKFGENFGYAYSFFVRFSSNLPWKETLSL-- 152
           GVD +++ + H+ M  FF  P   K +A RK GE  GYA SF  RF  NLPWKETLS   
Sbjct: 96  GVDERLLSTAHKLMDTFFKSPNYEKLKAQRKVGETTGYASSFVGRFKENLPWKETLSFSF 155

Query: 153 -PCVADGENSSSAYDYLIEKLGPTFSHHGKVYQDCAKAVNDLGMKILEVLGLSLGVSREY 212
            P       S +  +Y+ + +G  +   G VYQ+ A+ +++L +KI+E+LG+SLG+ RE+
Sbjct: 156 SPTEKSENYSQTVKNYISKTMGDGYKDFGSVYQEYAETMSNLSLKIMELLGMSLGIKREH 215

Query: 213 FKNFYEDNDSIMRLNYYPPCAKPELVLGTGPHCDPTSITILHQDKVSGFQVCVDDQWYSI 272
           F+ F+EDN+SI RLNYYP C +P+LVLGTGPHCDPTS+TIL QD+VSG QV VD+QW SI
Sbjct: 216 FREFFEDNESIFRLNYYPKCKQPDLVLGTGPHCDPTSLTILQQDQVSGLQVFVDNQWQSI 275

Query: 273 PPTPDSFVINIGDTFKSLTNGIYKSCVHRAVVNSENVRKTMAFFICPNHDKVVRAPEELV 332
           PP P + V+NIGDT  +LTNGIYKSC+HRAVVN E  RKT+AFF+CP  DKVV+ P EL 
Sbjct: 276 PPIPQALVVNIGDTLMALTNGIYKSCLHRAVVNGETTRKTLAFFLCPKVDKVVKPPSELE 335

Query: 333 EKNPPRKYPDYTWGTLLEITQKHYRPDCNTLEALTDWIQ 369
            +   R YPD+TW   LE T KHYR D NTLE  T+W++
Sbjct: 336 GE---RAYPDFTWSMFLEFTMKHYRADMNTLEEFTNWLK 371

BLAST of CmaCh18G012080.1 vs. TAIR10
Match: AT1G44090.1 (AT1G44090.1 gibberellin 20-oxidase 5)

HSP 1 Score: 301.2 bits (770), Expect = 9.0e-82
Identity = 150/340 (44.12%), Postives = 204/340 (60.00%), Query Frame = 1

Query: 30  ESKVPESFVWPDDLKGSQEVPVLNVPHIDLNKFLSGNESDVEEVTRLVDEACRKHGFFVV 89
           +  VP  F WP+      E   L++P IDL+ FL+GNE++ +   + V +AC  HG F+V
Sbjct: 40  QPNVPAEFFWPEKDVAPSEGD-LDLPIIDLSGFLNGNEAETQLAAKAVKKACMAHGTFLV 99

Query: 90  VNHGVDMKVMESLHECMSEFFSMPMDVKQRAHRKFGENFGYAYSFFVRFSSNLPWKETLS 149
           VNHG    + E   E  S FF +  D K RA+R  G   GY      RFSSNLPW ETL+
Sbjct: 100 VNHGFKSGLAEKALEISSLFFGLSKDEKLRAYRIPGNISGYTAGHSQRFSSNLPWNETLT 159

Query: 150 LPCVADGENSSSAYDYLIEKLGPTFSHHGKVYQDCAKAVNDLGMKILEVLGLSLGV-SRE 209
           L       +     D+L  +LG      G+V+Q+   A+N L M ++E+LG+S+G+  R 
Sbjct: 160 LAFKKGPPH--VVEDFLTSRLGNHRQEIGQVFQEFCDAMNGLVMDLMELLGISMGLKDRT 219

Query: 210 YFKNFYEDNDSIMRLNYYPPCAKPELVLGTGPHCDPTSITILHQDKVSGFQVCVDDQWYS 269
           Y++ F+ED   I R NYYPPC +PE  LG GPH DPT+IT+L QD V G +V     W +
Sbjct: 220 YYRRFFEDGSGIFRCNYYPPCKQPEKALGVGPHNDPTAITVLLQDDVVGLEVFAAGSWQT 279

Query: 270 IPPTPDSFVINIGDTFKSLTNGIYKSCVHRAVVNSENVRKTMAFFICPNHDKVVRAPEEL 329
           + P P + V+N+GDTF +L+NG Y+SC HRAVVN E VR+++ FF CP  DK++  P EL
Sbjct: 280 VRPRPGALVVNVGDTFMALSNGNYRSCYHRAVVNKEKVRRSLVFFSCPREDKIIVPPPEL 339

Query: 330 VE-KNPPRKYPDYTWGTLLEITQKHYRPDCNTLEALTDWI 368
           VE +   RKYPD+TW  L + TQ  YR D  TL   + W+
Sbjct: 340 VEGEEASRKYPDFTWAQLQKFTQSGYRVDNTTLHNFSSWL 376

BLAST of CmaCh18G012080.1 vs. NCBI nr
Match: gi|486625|emb|CAA51744.1| (gibberellin 20-oxidase [Cucurbita maxima])

HSP 1 Score: 595.1 bits (1533), Expect = 8.6e-167
Identity = 274/384 (71.35%), Postives = 327/384 (85.16%), Query Frame = 1

Query: 1   MATESLSSAHSDLNQETKGEYRGTFAGPDESKVPESFVWPDDLKGSQEVPVLNVPHIDLN 60
           +ATES   A S+LN+E KGEYR  F G DESKVPE F+W +  + S+ +PVL+VP IDL 
Sbjct: 7   VATES---APSNLNEEMKGEYRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVPTIDLE 66

Query: 61  KFLSGNESDVEEVTRLVDEACRKHGFFVVVNHGVDMKVMESLHECMSEFFSMPMDVKQRA 120
           KF+SG++S VEE TRLVDEACR+HG F VVNHGVD+++M  +H+CM+EFF+MP+DVKQRA
Sbjct: 67  KFMSGDKSYVEEATRLVDEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLDVKQRA 126

Query: 121 HRKFGENFGYAYSFFVRFSSNLPWKETLSLPCVADGENSSSAYDYLIEKLGPTFSHHGKV 180
            RK GE++GY  SFF RF+SNLPWKET SL CVA  +NSS+A+DY+++ LGP+FSHHGK 
Sbjct: 127 KRKVGESYGYTNSFFGRFASNLPWKETFSLRCVA-AQNSSAAHDYVLDTLGPSFSHHGKA 186

Query: 181 YQDCAKAVNDLGMKILEVLGLSLGVSREYFKNFYEDNDSIMRLNYYPPCAKPELVLGTGP 240
           YQ+C  A+N+LG KI+E+LGLSLG+SREYFKNF+EDNDSI+RLNYYP C KPE+VLGTGP
Sbjct: 187 YQECGIALNELGTKIVELLGLSLGISREYFKNFFEDNDSILRLNYYPTCDKPEVVLGTGP 246

Query: 241 HCDPTSITILHQDKVSGFQVCVDDQWYSIPPTPDSFVINIGDTFKSLTNGIYKSCVHRAV 300
           H DPTS+TILHQD VSG QVC +DQWYSIPP P++FVINIGDTF SLTNGIYK C+HRAV
Sbjct: 247 HTDPTSVTILHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHRAV 306

Query: 301 VNSENVRKTMAFFICPNHDKVVRAPEELVEKNPPRKYPDYTWGTLLEITQKHYRPDCNTL 360
           VNS N RK++AFF+CP+HDKVVRAPEELVEK+PPRKYPDY W  LLE+TQK YRPDCNTL
Sbjct: 307 VNSMNARKSLAFFLCPSHDKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRPDCNTL 366

Query: 361 EALTDWIQKGKPLDNGSTITAPSA 385
           EA   W+Q+GK LD GSTITAPSA
Sbjct: 367 EAFKTWVQEGKALDTGSTITAPSA 386

BLAST of CmaCh18G012080.1 vs. NCBI nr
Match: gi|2224890|gb|AAB64345.1| (gibberellin 20-oxidase [Cucurbita maxima])

HSP 1 Score: 589.0 bits (1517), Expect = 6.2e-165
Identity = 272/384 (70.83%), Postives = 325/384 (84.64%), Query Frame = 1

Query: 1   MATESLSSAHSDLNQETKGEYRGTFAGPDESKVPESFVWPDDLKGSQEVPVLNVPHIDLN 60
           +ATES   A S+LN+E KGEYR  F G DESKVPE F+W +  + S+ +PVL+VP IDL 
Sbjct: 7   VATES---APSNLNEEMKGEYRPPFGGSDESKVPEDFIWSEKFEASELLPVLDVPTIDLE 66

Query: 61  KFLSGNESDVEEVTRLVDEACRKHGFFVVVNHGVDMKVMESLHECMSEFFSMPMDVKQRA 120
           KF+SG++S VEE TRLVDEACR+HG F VVNHGVD+++M  +H+CM+EFF+MP+DVKQRA
Sbjct: 67  KFMSGDKSYVEEATRLVDEACRQHGIFFVVNHGVDIEMMGRVHDCMNEFFTMPLDVKQRA 126

Query: 121 HRKFGENFGYAYSFFVRFSSNLPWKETLSLPCVADGENSSSAYDYLIEKLGPTFSHHGKV 180
            RK GE++GY  SFF RF+SNLPWKET SL CVA  +NSS+A+DY+++ LG +FSHHGK 
Sbjct: 127 KRKVGESYGYTNSFFGRFASNLPWKETFSLRCVA-AQNSSAAHDYVLDTLGSSFSHHGKA 186

Query: 181 YQDCAKAVNDLGMKILEVLGLSLGVSREYFKNFYEDNDSIMRLNYYPPCAKPELVLGTGP 240
           YQ+C  A+N+LG KI+E+LGLSLG+SREYFKNFY+DNDSI+RLNYYP C KPE+VLGTGP
Sbjct: 187 YQECGIALNELGTKIVELLGLSLGISREYFKNFYKDNDSILRLNYYPTCDKPEVVLGTGP 246

Query: 241 HCDPTSITILHQDKVSGFQVCVDDQWYSIPPTPDSFVINIGDTFKSLTNGIYKSCVHRAV 300
           H DPTS+TILHQD VSG QVC +DQWYSIPP P++FVINIGDTF SLTNGIYK C+HRAV
Sbjct: 247 HTDPTSVTILHQDPVSGLQVCSNDQWYSIPPNPEAFVINIGDTFTSLTNGIYKGCIHRAV 306

Query: 301 VNSENVRKTMAFFICPNHDKVVRAPEELVEKNPPRKYPDYTWGTLLEITQKHYRPDCNTL 360
           VNS N RK++AFF+CP+HDKVVRAPEELVEK+PPRKYPDY W  LLE+TQK YRP CNTL
Sbjct: 307 VNSMNARKSLAFFLCPSHDKVVRAPEELVEKSPPRKYPDYKWPMLLEMTQKRYRPACNTL 366

Query: 361 EALTDWIQKGKPLDNGSTITAPSA 385
           EA   W+Q+GK LD GSTITAPSA
Sbjct: 367 EAFKTWVQEGKALDTGSTITAPSA 386

BLAST of CmaCh18G012080.1 vs. NCBI nr
Match: gi|1666094|emb|CAA70329.1| (gibberellin 20-oxidase [Marah macrocarpus])

HSP 1 Score: 545.4 bits (1404), Expect = 7.8e-152
Identity = 251/369 (68.02%), Postives = 299/369 (81.03%), Query Frame = 1

Query: 1   MATESLSSAHSDLNQETKGEYRGTFAGPDESKVPESFVWPDDLKGSQEVPVLNVPHIDLN 60
           MA   + +  S   +E K E++  F G  ES VPE+F+WPD+ K + + P L+VPHIDL 
Sbjct: 1   MAITCMMATESSPAEEVKREHQVAFGGAGESSVPENFIWPDEFKATADAPELHVPHIDLK 60

Query: 61  KFLSGNESDVEEVTRLVDEACRKHGFFVVVNHGVDMKVMESLHECMSEFFSMPMDVKQRA 120
           K LSG+E DVEE TRLVDEACRKHGFFVVVNHGVD ++M  +HECM+EFF++P+DVKQ+A
Sbjct: 61  KVLSGDEKDVEEATRLVDEACRKHGFFVVVNHGVDKELMNKVHECMNEFFTLPLDVKQKA 120

Query: 121 HRKFGENFGYAYSFFVRFSSNLPWKETLSLPCVADGENSSSAYDYLIEKLGPTFSHHGKV 180
           HRK GENFGYA SF  RFS+ LPWKET SL  +A  ENSS+A DY+ + LGP FSHHG+V
Sbjct: 121 HRKVGENFGYANSFIGRFSTKLPWKETFSLRYLAH-ENSSTARDYVSQVLGPEFSHHGEV 180

Query: 181 YQDCAKAVNDLGMKILEVLGLSLGVSREYFKNFYEDNDSIMRLNYYPPCAKPELVLGTGP 240
           YQ+C KA++DL ++I+E+LGLSLG+SRE F+ FYEDNDSIMR+NYYP C KPEL LGTGP
Sbjct: 181 YQECGKALSDLSLRIVELLGLSLGISRETFRKFYEDNDSIMRMNYYPRCEKPELTLGTGP 240

Query: 241 HCDPTSITILHQDKVSGFQVCVDDQWYSIPPTPDSFVINIGDTFKSLTNGIYKSCVHRAV 300
           HCDPTSITILHQD VSG QV VDDQW+SIPPT DSFV+N+GDTF SLTNG+YKSC HRAV
Sbjct: 241 HCDPTSITILHQDDVSGLQVYVDDQWHSIPPTEDSFVVNVGDTFMSLTNGVYKSCFHRAV 300

Query: 301 VNSENVRKTMAFFICPNHDKVVRAPEELVEKNPPRKYPDYTWGTLLEITQKHYRPDCNTL 360
           VN +  RK+MAFF+CP  +KVVRAPEELVEK PPRK+PDYTW  LLE+TQK+YR D NT 
Sbjct: 301 VNCKEARKSMAFFLCPAVEKVVRAPEELVEKYPPRKFPDYTWPMLLEMTQKYYRADSNTF 360

Query: 361 EALTDWIQK 370
            A T WIQ+
Sbjct: 361 NAFTTWIQQ 368

BLAST of CmaCh18G012080.1 vs. NCBI nr
Match: gi|5359492|gb|AAD42693.1|AF074709_1 (gibberellin 20-oxidase [Citrullus lanatus subsp. vulgaris])

HSP 1 Score: 539.3 bits (1388), Expect = 5.6e-150
Identity = 252/383 (65.80%), Postives = 307/383 (80.16%), Query Frame = 1

Query: 1   MATESLSSAHSDLNQETKGEYRGTFAGPDESKVPESFVWPDDLKGSQEVPVLNVPHIDLN 60
           MA + + S       ++K  +R  F   +ES VPE+FVWPD+ K     P L+VPHIDL+
Sbjct: 1   MAMKCMMSTGESKEMKSK-LHRVEFGCSEESMVPENFVWPDEFKAKDGAPELHVPHIDLH 60

Query: 61  KFLSGNESDVEEVTRLVDEACRKHGFFVVVNHGVDMKVMESLHECMSEFFSMPMDVKQRA 120
           KFL+GNESD+EE TRLVDEACRKHGFFV+VNHGVDM++++ +HECM EFF++P DVKQ++
Sbjct: 61  KFLNGNESDIEEATRLVDEACRKHGFFVLVNHGVDMELIKGVHECMDEFFTLPFDVKQKS 120

Query: 121 HRKFGENFGYAYSFFVRFSSNLPWKETLSLPCVADGENSSSAYDYLIEKLGPTFSHHGKV 180
            RKFGEN+GYA SF  RFS+NLPWKETLSLP VA+  NS+S  D++ + +GP  SH GKV
Sbjct: 121 QRKFGENYGYANSFIGRFSNNLPWKETLSLPYVAN-HNSTSVQDFVSKCVGPELSHQGKV 180

Query: 181 YQDCAKAVNDLGMKILEVLGLSLGVSREYFKNFYEDNDSIMRLNYYPPCAKPELVLGTGP 240
           YQ+C KA++DL +KI+E+LGLSLGV +E FK FYEDNDSI+RLNYYPPC KPEL LGTGP
Sbjct: 181 YQECGKALSDLSLKIVELLGLSLGVPKEKFKKFYEDNDSIIRLNYYPPCEKPELTLGTGP 240

Query: 241 HCDPTSITILHQDKVSGFQVCVDDQWYSIPPTPDSFVINIGDTFKSLTNGIYKSCVHRAV 300
           HCDPTSITILHQD VSG QV VDD+W+SIPPT DSFVINIGDTF +LTNG+YKSC HRAV
Sbjct: 241 HCDPTSITILHQDHVSGLQVYVDDEWHSIPPTKDSFVINIGDTFMALTNGVYKSCFHRAV 300

Query: 301 VNSENVRKTMAFFICPNHDKVVRAPEELVEKNPPRKYPDYTWGTLLEITQKHYRPDCNTL 360
           VN + VRK+MAFF+CP  DKVVRAP+E+VEKNPPRK+PDYTW  LLE+TQK YR D NT 
Sbjct: 301 VNCKEVRKSMAFFLCPAADKVVRAPDEVVEKNPPRKFPDYTWPMLLELTQKFYRADSNTF 360

Query: 361 EALTDWIQKGKPLDNGSTITAPS 384
           +A   W++K K   + +TI  P+
Sbjct: 361 QAFIPWLEKQK---SANTIAPPA 378

BLAST of CmaCh18G012080.1 vs. NCBI nr
Match: gi|384368293|emb|CBL59326.1| (Gibberellin 20-oxidase [Cucurbita maxima])

HSP 1 Score: 531.6 bits (1368), Expect = 1.2e-147
Identity = 248/381 (65.09%), Postives = 297/381 (77.95%), Query Frame = 1

Query: 3   TESLSSAHSDLNQETKGEYRGTFAGPDESKVPESFVWPDDLKGSQEVPVLNVPHIDLNKF 62
           T + S   S L QE K ++R  F   DES VPE+F+WPD+ K  + VP L+VPHIDL KF
Sbjct: 7   TTAKSQGESKLKQEMKRKHRVEFGSSDESMVPENFIWPDEFKARESVPELDVPHIDLQKF 66

Query: 63  LSGNESDVEEVTRLVDEACRKHGFFVVVNHGVDMKVMESLHECMSEFFSMPMDVKQRAHR 122
           +S NE+D+EE TRLVDEACRKHGFFV+VNHGVDM++M+++HECM EFFS+P+DVKQ+A R
Sbjct: 67  VSENENDIEEATRLVDEACRKHGFFVLVNHGVDMELMKNVHECMDEFFSLPLDVKQKAQR 126

Query: 123 KFGENFGYAYSFFVRFSSNLPWKETLSLPCVADGENSSSAYDYLIEKLGPTFSHHGKVYQ 182
           K GEN+GYA SF  RFSS LPWKET SL   A   N    +DY+   LGP FS  GKVY+
Sbjct: 127 KVGENYGYANSFIGRFSSKLPWKETFSLRYAAH-HNLPVTHDYVCHHLGPEFSQQGKVYE 186

Query: 183 DCAKAVNDLGMKILEVLGLSLGVSREYFKNFYEDNDSIMRLNYYPPCAKPELVLGTGPHC 242
           +C +A++DL +KI+E+LGLSLGV +E FK F+EDNDSI+RLNYYPPC KPEL LGTGPHC
Sbjct: 187 ECGEALSDLSLKIVELLGLSLGVPKEKFKKFFEDNDSIIRLNYYPPCNKPELTLGTGPHC 246

Query: 243 DPTSITILHQDKVSGFQVCVDDQWYSIPPTPDSFVINIGDTFKSLTNGIYKSCVHRAVVN 302
           DPTSITILHQD VSG QV VDDQW+SIPP  DSFVINIGDTF +LTNG+YKSC HRAVVN
Sbjct: 247 DPTSITILHQDHVSGLQVYVDDQWHSIPPIKDSFVINIGDTFMALTNGVYKSCFHRAVVN 306

Query: 303 SENVRKTMAFFICPNHDKVVRAPEELVEKNPPRKYPDYTWGTLLEITQKHYRPDCNTLEA 362
               RK++ FF+CP  DKVVRAP+E+V+KNPPRK+PDYTW  LLE+TQK YR D NT +A
Sbjct: 307 CREARKSIVFFLCPADDKVVRAPDEIVDKNPPRKFPDYTWPMLLELTQKFYRSDSNTFKA 366

Query: 363 LTDWIQKGKPLDNGSTITAPS 384
            T W+Q  K L   ++  APS
Sbjct: 367 FTTWLQH-KNLATAASSLAPS 385