CmaCh17G012680.1 (mRNA) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh17G012680.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Invertase/pectin methylesterase inhibitor family protein
Location	Cma_Chr17 : 8612071 .. 8612616 (-)
Sequence length	546

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCATATTCTAGTTTTCTTGTAGTCTCCTCTCTTTTAGCTTTCCTTTCACTTCTCTTCTTCAATGGGGTCGTGTCCATTCATGCTGCTTCCCCAGATGACGCCACTTCCACCATCTGCAAGAAAGCTACAAATCCTTCTTTTTGCTTTAACGTCTTGAAATCTGTTGGCACCACAGACCTAAAAGGACTGGCCATCTTCACCCTCAACCTCGCCCACGACAAGACTATTGGAAACCGTGCCCTCGCCCAGTCCCTAGCGTCCAAGGTCGCGGATCCCGAGCTTAAGGACCGCTATGACACTTGTGTCGAACGCTACAACGATGCCGTCAAGGACATCGAGGGTGCGAAGGGCTACTTGGGGAAAGGTGACTATAAAGGCGTCTACACTTATACTTTTGGAGCCATGACGGAGGTCGATGCCTGTCTGTTCCGCCTTTCGAAGCTGCCAAAGGACTCATCGGCACTCCCAGGCAACGGGAAAGCCGTGGAAGATATTTATAGTATCATCTTGGTTATAGCCAATCTTCTTCTTGGGCATGCTTAA

mRNA sequence

ATGGCATATTCTAGTTTTCTTGTAGTCTCCTCTCTTTTAGCTTTCCTTTCACTTCTCTTCTTCAATGGGGTCGTGTCCATTCATGCTGCTTCCCCAGATGACGCCACTTCCACCATCTGCAAGAAAGCTACAAATCCTTCTTTTTGCTTTAACGTCTTGAAATCTGTTGGCACCACAGACCTAAAAGGACTGGCCATCTTCACCCTCAACCTCGCCCACGACAAGACTATTGGAAACCGTGCCCTCGCCCAGTCCCTAGCGTCCAAGGTCGCGGATCCCGAGCTTAAGGACCGCTATGACACTTGTGTCGAACGCTACAACGATGCCGTCAAGGACATCGAGGGTGCGAAGGGCTACTTGGGGAAAGGTGACTATAAAGGCGTCTACACTTATACTTTTGGAGCCATGACGGAGGTCGATGCCTGTCTGTTCCGCCTTTCGAAGCTGCCAAAGGACTCATCGGCACTCCCAGGCAACGGGAAAGCCGTGGAAGATATTTATAGTATCATCTTGGTTATAGCCAATCTTCTTCTTGGGCATGCTTAA

Coding sequence (CDS)

ATGGCATATTCTAGTTTTCTTGTAGTCTCCTCTCTTTTAGCTTTCCTTTCACTTCTCTTCTTCAATGGGGTCGTGTCCATTCATGCTGCTTCCCCAGATGACGCCACTTCCACCATCTGCAAGAAAGCTACAAATCCTTCTTTTTGCTTTAACGTCTTGAAATCTGTTGGCACCACAGACCTAAAAGGACTGGCCATCTTCACCCTCAACCTCGCCCACGACAAGACTATTGGAAACCGTGCCCTCGCCCAGTCCCTAGCGTCCAAGGTCGCGGATCCCGAGCTTAAGGACCGCTATGACACTTGTGTCGAACGCTACAACGATGCCGTCAAGGACATCGAGGGTGCGAAGGGCTACTTGGGGAAAGGTGACTATAAAGGCGTCTACACTTATACTTTTGGAGCCATGACGGAGGTCGATGCCTGTCTGTTCCGCCTTTCGAAGCTGCCAAAGGACTCATCGGCACTCCCAGGCAACGGGAAAGCCGTGGAAGATATTTATAGTATCATCTTGGTTATAGCCAATCTTCTTCTTGGGCATGCTTAA

Protein sequence

MAYSSFLVVSSLLAFLSLLFFNGVVSIHAASPDDATSTICKKATNPSFCFNVLKSVGTTDLKGLAIFTLNLAHDKTIGNRALAQSLASKVADPELKDRYDTCVERYNDAVKDIEGAKGYLGKGDYKGVYTYTFGAMTEVDACLFRLSKLPKDSSALPGNGKAVEDIYSIILVIANLLLGHA

Homology

[Close]

BLAST of CmaCh17G012680.1 vs. Swiss-Prot
Match: PMEI_ACTDE (Pectinesterase inhibitor OS=Actinidia deliciosa GN=PMEI PE=1 SV=2)

HSP 1 Score: 90.1 bits (222), Expect = 2.6e-17
Identity = 62/179 (34.64%), Postives = 86/179 (48.04%), Query Frame = 1


Query: 4   SSFLVVSSLLAFLSLLFFNGVVSIHAASPDDATSTICKKATNPSFCFNVLKS---VGTTD 63
           SS L VS LL  L +   +   S+ A +     S IC K  NPS C   L+S     + D
Sbjct: 7   SSSLFVSLLLVILFISPLSQRPSVKAEN--HLISEICPKTRNPSLCLQALESDPRSASKD 66

Query: 64  LKGLAIFTLNLAHDKTIGNRALAQSLASKVADPELKDRYDTCVERYNDAVKDIEGAKGYL 123
           LKGL  F++++A         +  SL ++  DP+LK RY+TC E Y DA+  +  AK +L
Sbjct: 67  LKGLGQFSIDIAQASAKQTSKIIASLTNQATDPKLKGRYETCSENYADAIDSLGQAKQFL 126

Query: 124 GKGDYKGVYTYTFGAMTEVDACLFRLSKLPKDSSALPGNGKAVEDIYSIILVIANLLLG 180
             GDY  +  Y   A      C       P   + L      +ED+  I+LVI+NLL G
Sbjct: 127 TSGDYNSLNIYASAAFDGAGTCEDSFEGPPNIPTQLHQADLKLEDLCDIVLVISNLLPG 183

[Close]

BLAST of CmaCh17G012680.1 vs. Swiss-Prot
Match: PMEI1_ARATH (Pectinesterase inhibitor 1 OS=Arabidopsis thaliana GN=PMEI1 PE=1 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 2.5e-15
Identity = 53/165 (32.12%), Postives = 80/165 (48.48%), Query Frame = 1


Query: 14  AFLSLLFFNGVVSIHAASPDDATSTICKKATNPSFCFNVLKS-VGTTDLKGLAIFTLNLA 73
           AFLS L F  ++    A      STIC K  NPSFC   L +   + +L+ LA  TL+  
Sbjct: 9   AFLSSLMFLLLIGSSYAITSSEMSTICDKTLNPSFCLKFLNTKFASPNLQALAKTTLDST 68

Query: 74  HDKTIGNRALAQSLASKVADPELKDRYDTCVERYNDAVKDIEGAKGYLGKGDYKGVYTYT 133
             +        QS+     DP  K  Y +CV+ Y  A+ ++E A  +L  GD  G+    
Sbjct: 69  QARATQTLKKLQSIIDGGVDPRSKLAYRSCVDEYESAIGNLEEAFEHLASGDGMGMNMKV 128

Query: 134 FGAMTEVDACLFRLSKLPKDSSALPGNGKAVEDIYSIILVIANLL 178
             A+   D CL  + +L    S++  N K ++++  I LVI+N+L
Sbjct: 129 SAALDGADTCLDDVKRLRSVDSSVVNNSKTIKNLCGIALVISNML 173

[Close]

BLAST of CmaCh17G012680.1 vs. Swiss-Prot
Match: PMEI2_ARATH (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 4.0e-13
Identity = 54/164 (32.93%), Postives = 77/164 (46.95%), Query Frame = 1


Query: 17  SLLFFNGVVSIHAASPDDATSTICKKATNPSFCFNVLKSVGTT---DLKGLAIFTLNLAH 76
           SL+FF    S++A   D     IC KA N SFC + +KS   T   DL+ LA  T   A 
Sbjct: 13  SLMFFVMTGSLNAQVAD--IKAICGKAKNQSFCTSYMKSNPKTSGADLQTLANITFGSAQ 72

Query: 77  DKTIGNRALAQSLASKVADPELKDRYDTCVERYNDAVKDIEGAKGYLGKGDYKGVYTYTF 136
                     QSL     +P +K  Y +CV+ Y  A+  +  AK  L  GD KG+     
Sbjct: 73  TSASEGFRKIQSLVKTATNPTMKKAYTSCVQHYKSAISSLNDAKQSLASGDGKGLNIKVS 132

Query: 137 GAMTEVDACLFRLSKLPKDSSALPGNGKAVEDIYSIILVIANLL 178
            AM     C   ++    D SA+  +G   ++I  I+LVI+N++
Sbjct: 133 AAMEGPSTCEQDMADFKVDPSAVKNSGD-FQNICGIVLVISNMM 173

[Close]

BLAST of CmaCh17G012680.1 vs. TrEMBL
Match: A0A0A0KCV8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G307340 PE=4 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 8.2e-34
Identity = 84/176 (47.73%), Postives = 110/176 (62.50%), Query Frame = 1


Query: 1   MAYSSFLVVSSLLAFLSLLFFNGVVSIHAASPDDATSTICKKATNPSFCFNVLKSVGTTD 60
           MA +S  +  SL+  L  +  +     + AS +D  STIC K +NP FC +VLKS GTTD
Sbjct: 1   MANNSCFITVSLIGVLLFIIIS-----YVASSNDVISTICPKTSNPPFCSSVLKSAGTTD 60

Query: 61  LKGLAIFTLNLAHDKTIGNRALAQSLASKVADPELKDRYDTCVERYNDAVKDIEGAKGYL 120
           LKGL ++TLNLAH     +  LA SLA    +P+LK RY +CVE Y++AV DIE A+  L
Sbjct: 61  LKGLVVYTLNLAHTNAGKSLTLANSLAKTATNPQLKQRYSSCVESYDEAVGDIENAQKDL 120

Query: 121 GKGDYKGVYTYTFGAMTEVDACLFRLSKLPKDSSALPGNGKAVEDIYSIILVIANL 177
              D+ GV   T GAMT +  C  + ++ PKD+S L  NGK + DI SIILVI+NL
Sbjct: 121 AVEDFNGVNIVTSGAMTNIGDCQDKFAQPPKDTSLLLENGKTINDICSIILVISNL 171

[Close]

BLAST of CmaCh17G012680.1 vs. TrEMBL
Match: A0A0A0KEW6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G307350 PE=4 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 2.7e-29
Identity = 78/168 (46.43%), Postives = 105/168 (62.50%), Query Frame = 1


Query: 11  SLLAFLSLLFFNGVVSIHAASP-DDATSTICKKATNPSFCFNVLKSVGTTDLKGLAIFTL 70
           +++   S LFF+ V S    +P +D  S+IC K  NP FC +VLKS G+TDLK LA +TL
Sbjct: 15  AIVFVFSFLFFSVVPSRATTTPSNDVASSICPKTRNPPFCVDVLKSAGSTDLKVLATYTL 74

Query: 71  NLAHDKTIGNRALAQSLASKVADPELKDRYDTCVERYNDAVKDIEGAKGYLGKGDYKGVY 130
           NLA++  + +  LA+SLA+   +P LK++Y +C E Y +A  DIE AK  L  GD+ GV 
Sbjct: 75  NLANENALKSTNLAKSLAAMTTNPPLKNQYLSCYESYEEATSDIENAKSNLASGDFNGVN 134

Query: 131 TYTFGAMTEVDACLFRLSKLPKDSSALPGNGKAVEDIYSIILVIANLL 178
             T G MT V  CL    +L  D S L  +GK + D+ SIILVI+NLL
Sbjct: 135 IATSGVMTSVSDCLDSFKQLRIDPSLLK-DGKTLNDVCSIILVISNLL 181

[Close]

BLAST of CmaCh17G012680.1 vs. TrEMBL
Match: A0A0A0KHY0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G307370 PE=4 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 4.2e-22
Identity = 73/178 (41.01%), Postives = 97/178 (54.49%), Query Frame = 1


Query: 4   SSFLVVSSLLAFLSLLFFNGVVSIHA--ASPDDATSTICKKATNPSFCFNVLKSVGTTD- 63
           S+  VVSS+L FL  LFF  VV +HA   SP+  T+TIC K  N +FC  VLK VG +D 
Sbjct: 12  SNLGVVSSILIFLFGLFFFSVVLLHADTGSPNVLTTTICPKTRNSTFCKTVLKPVGKSDA 71

Query: 64  -LKGLAIFTLNLAHDKTIGNRALAQSLASKVADPELKDRYDTCVERYNDAVKDIEGAKGY 123
            L  +A +TL  AH  T+     AQ LA++  DP LK RY  C  R++   K +E A   
Sbjct: 72  ALLKVANYTLTFAHTTTVEGLHHAQRLATEATDPLLKQRYSECSRRFDFVTKGLEEAIEA 131

Query: 124 LGKGDYKGVYTYTFGAMTEVDACLFRLSKLPKDSSALPGNGKAVEDIYSIILVIANLL 178
           L KG Y  +   T  A+ E D C+    K P + S LP   K + DI  I + ++N+L
Sbjct: 132 LAKGGYIPLSHATGAAVVEADRCVNMFKKPPPEPSKLPEKAKNIGDICDIAVSVSNIL 189

[Close]

BLAST of CmaCh17G012680.1 vs. TrEMBL
Match: A0A0A0KCC4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G307360 PE=4 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.4e-20
Identity = 70/178 (39.33%), Postives = 99/178 (55.62%), Query Frame = 1


Query: 8   VVSSLLAFLSLLF----FNGVVSIHA----ASPDDATSTICKKATNPSFCFNVLKSVGTT 67
           ++SS L F   LF     N V+ ++A    ++ D   S IC + +NPSFC ++L++    
Sbjct: 1   MISSNLNFGVFLFGFLLSNNVIPLNADTILSNIDTLASNICPQTSNPSFCASILENANNI 60

Query: 68  DLKGLAIFTLNLAHDKTIGNRALAQSLASKVADPELKDRYDTCVERYNDAVKDIEGAKGY 127
           DLK L  ++L LAH     +  LA++LA    +  LK +Y  C E Y++A+ DIE AK  
Sbjct: 61  DLKALIAYSLKLAHTNAGKSMTLAKALAVLTTNTLLKKQYLFCFENYDEAMCDIEKAKND 120

Query: 128 LGKGDYKGVYTYTFGAMTEVDACLFRLSKLPKDSSALPGNGKAVEDIYSIILVIANLL 178
           L  GDY GV      AM   D C     +  KD S LP N K ++DIYSIILVI+++L
Sbjct: 121 LVFGDYSGVNLAISDAMMIADDCHDSFKQPLKDMSLLPNNTKMLKDIYSIILVISSIL 178

[Close]

BLAST of CmaCh17G012680.1 vs. TrEMBL
Match: G7JFU8_MEDTR (Pectin methylesterase inhibitor OS=Medicago truncatula GN=MTR_4g025010 PE=4 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 5.0e-15
Identity = 64/173 (36.99%), Postives = 93/173 (53.76%), Query Frame = 1


Query: 16  LSLLFFNGVVSIHAASPDDATSTICKKATNPSFCFNVLKS----VGTTDLKGLAIFTLNL 75
           L ++F + V S +AA+  D   TICKKA +PSFC N LKS    VG  DL  L  +TL +
Sbjct: 9   LVIVFLSCVASSYAANVADP-QTICKKAKDPSFCLNFLKSKPGGVGR-DLNSLLKYTLGV 68

Query: 76  AHDKTIGNRALAQSLASKVA-DPELKDRYDTCVERYND---AVKDIEGAKGYLGKGDYKG 135
                 G  +L   L ++   D E K+ Y +C+  Y +   A+ +IE A+  L + DY G
Sbjct: 69  LRTDVSGTISLITKLIAQSGNDLEKKNYYKSCLTFYAEDEGALGEIEEAQDLLNRSDYNG 128

Query: 136 VYTYTFGAMTEVDACLFRLSKLPKDSSALPGNGKAVEDIYSIILVIANLLLGH 181
           V  +  G M++V  C        +D+S+LP     V ++  IIL+I+NLLL H
Sbjct: 129 VNVHISGVMSDVYNCGTGDEHPYQDTSSLPKQADVVNNVAEIILIISNLLLNH 179

[Close]

BLAST of CmaCh17G012680.1 vs. TAIR10
Match: AT1G48020.1 (AT1G48020.1 pectin methylesterase inhibitor 1)

HSP 1 Score: 83.6 bits (205), Expect = 1.4e-16
Identity = 53/165 (32.12%), Postives = 80/165 (48.48%), Query Frame = 1


Query: 14  AFLSLLFFNGVVSIHAASPDDATSTICKKATNPSFCFNVLKS-VGTTDLKGLAIFTLNLA 73
           AFLS L F  ++    A      STIC K  NPSFC   L +   + +L+ LA  TL+  
Sbjct: 9   AFLSSLMFLLLIGSSYAITSSEMSTICDKTLNPSFCLKFLNTKFASPNLQALAKTTLDST 68

Query: 74  HDKTIGNRALAQSLASKVADPELKDRYDTCVERYNDAVKDIEGAKGYLGKGDYKGVYTYT 133
             +        QS+     DP  K  Y +CV+ Y  A+ ++E A  +L  GD  G+    
Sbjct: 69  QARATQTLKKLQSIIDGGVDPRSKLAYRSCVDEYESAIGNLEEAFEHLASGDGMGMNMKV 128

Query: 134 FGAMTEVDACLFRLSKLPKDSSALPGNGKAVEDIYSIILVIANLL 178
             A+   D CL  + +L    S++  N K ++++  I LVI+N+L
Sbjct: 129 SAALDGADTCLDDVKRLRSVDSSVVNNSKTIKNLCGIALVISNML 173

[Close]

BLAST of CmaCh17G012680.1 vs. TAIR10
Match: AT3G17220.1 (AT3G17220.1 pectin methylesterase inhibitor 2)

HSP 1 Score: 76.3 bits (186), Expect = 2.2e-14
Identity = 54/164 (32.93%), Postives = 77/164 (46.95%), Query Frame = 1


Query: 17  SLLFFNGVVSIHAASPDDATSTICKKATNPSFCFNVLKSVGTT---DLKGLAIFTLNLAH 76
           SL+FF    S++A   D     IC KA N SFC + +KS   T   DL+ LA  T   A 
Sbjct: 13  SLMFFVMTGSLNAQVAD--IKAICGKAKNQSFCTSYMKSNPKTSGADLQTLANITFGSAQ 72

Query: 77  DKTIGNRALAQSLASKVADPELKDRYDTCVERYNDAVKDIEGAKGYLGKGDYKGVYTYTF 136
                     QSL     +P +K  Y +CV+ Y  A+  +  AK  L  GD KG+     
Sbjct: 73  TSASEGFRKIQSLVKTATNPTMKKAYTSCVQHYKSAISSLNDAKQSLASGDGKGLNIKVS 132

Query: 137 GAMTEVDACLFRLSKLPKDSSALPGNGKAVEDIYSIILVIANLL 178
            AM     C   ++    D SA+  +G   ++I  I+LVI+N++
Sbjct: 133 AAMEGPSTCEQDMADFKVDPSAVKNSGD-FQNICGIVLVISNMM 173

[Close]

BLAST of CmaCh17G012680.1 vs. NCBI nr
Match: gi|659128306|ref|XP_008464144.1| (PREDICTED: pectinesterase inhibitor-like [Cucumis melo])

HSP 1 Score: 164.1 bits (414), Expect = 2.3e-37
Identity = 90/177 (50.85%), Postives = 115/177 (64.97%), Query Frame = 1


Query: 1   MAYSSFLVVSSLLAFLSLLFFNGVVSIHAASPDDATSTICKKATNPSFCFNVLKSVGTTD 60
           M Y+S L + SL+  L       + +I+ AS  D  STIC K +NP FC +VLKS GTTD
Sbjct: 9   MVYNSCLFIVSLIGVL-------LFTINVASSTDVVSTICPKTSNPQFCSSVLKSAGTTD 68

Query: 61  LKGLAIFTLNLAHDKTIGNRALAQSLASKVADPELKDRYDTCVERYNDAVKDIEGAKGYL 120
           LKGLA++TLNLAH     +  LA SLA    +P+LK RY +CVE Y++AV DIE  +  L
Sbjct: 69  LKGLAVYTLNLAHTNARKSLTLANSLAKTTTNPQLKQRYSSCVESYDEAVGDIENVQKDL 128

Query: 121 GKGDYKGVYTYTFGAMTEVDACLFRLSKLPKDSSALPGNGKAVEDIYSIILVIANLL 178
             GD+ GV   T GAMTE+D C  + ++ PKD+S L  NGK + DI SIILVI+NLL
Sbjct: 129 ALGDFNGVNIVTSGAMTEIDDCQDKFAQPPKDTSLLLKNGKTLNDICSIILVISNLL 178

[Close]

BLAST of CmaCh17G012680.1 vs. NCBI nr
Match: gi|659113905|ref|XP_008456811.1| (PREDICTED: pectinesterase inhibitor-like [Cucumis melo])

HSP 1 Score: 161.4 bits (407), Expect = 1.5e-36
Identity = 88/177 (49.72%), Postives = 116/177 (65.54%), Query Frame = 1


Query: 1   MAYSSFLVVSSLLAFLSLLFFNGVVSIHAASPDDATSTICKKATNPSFCFNVLKSVGTTD 60
           MA +S L++ SL+  L     + V     AS +D  STIC K +NPSFC +VLKS GTTD
Sbjct: 1   MANNSCLIIVSLVGVLLFTIISNV-----ASSNDVVSTICPKTSNPSFCSSVLKSAGTTD 60

Query: 61  LKGLAIFTLNLAHDKTIGNRALAQSLASKVADPELKDRYDTCVERYNDAVKDIEGAKGYL 120
           LKGLA++TLNLAH     +  LA+ LA+   +P+LK RY +C E Y++ V DIE A+  L
Sbjct: 61  LKGLAVYTLNLAHTNAHKSLTLAKLLATTTTNPQLKQRYSSCAESYDETVGDIENAQKDL 120

Query: 121 GKGDYKGVYTYTFGAMTEVDACLFRLSKLPKDSSALPGNGKAVEDIYSIILVIANLL 178
             GD+  V   T GAMTE+D C  + ++ PKD+S L  NGK + DIY+IILVI+NLL
Sbjct: 121 ALGDFNAVNIVTSGAMTEIDDCQDKFAQPPKDTSLLLKNGKTLNDIYNIILVISNLL 172

[Close]

BLAST of CmaCh17G012680.1 vs. NCBI nr
Match: gi|659117593|ref|XP_008458683.1| (PREDICTED: pectinesterase inhibitor [Cucumis melo])

HSP 1 Score: 159.8 bits (403), Expect = 4.3e-36
Identity = 89/177 (50.28%), Postives = 115/177 (64.97%), Query Frame = 1


Query: 1   MAYSSFLVVSSLLAFLSLLFFNGVVSIHAASPDDATSTICKKATNPSFCFNVLKSVGTTD 60
           MA +S LV+ SL+  L       + +I+ AS  D  STIC K +NP FC +VLKS GTTD
Sbjct: 1   MANNSCLVIVSLIGVL-------LFTINVASSTDIVSTICPKTSNPPFCSSVLKSAGTTD 60

Query: 61  LKGLAIFTLNLAHDKTIGNRALAQSLASKVADPELKDRYDTCVERYNDAVKDIEGAKGYL 120
           LKGLA++TLN AH     +  LA SLA    +P+LK RY +C E Y++A+ DIE A+  L
Sbjct: 61  LKGLAVYTLNFAHTNARKSLTLASSLAKTTPNPQLKQRYSSCTESYDEAIGDIENAQKDL 120

Query: 121 GKGDYKGVYTYTFGAMTEVDACLFRLSKLPKDSSALPGNGKAVEDIYSIILVIANLL 178
             GD+ GV   T GAMT +D C  +L++ PKD+S L  NGK + DI SIILVI+NLL
Sbjct: 121 ALGDFNGVNIVTSGAMTGIDDCQDKLAQPPKDTSLLLKNGKTLNDICSIILVISNLL 170

[Close]

BLAST of CmaCh17G012680.1 vs. NCBI nr
Match: gi|659131349|ref|XP_008465639.1| (PREDICTED: pectinesterase inhibitor-like [Cucumis melo])

HSP 1 Score: 156.8 bits (395), Expect = 3.7e-35
Identity = 90/177 (50.85%), Postives = 113/177 (63.84%), Query Frame = 1


Query: 1   MAYSSFLVVSSLLAFLSLLFFNGVVSIHAASPDDATSTICKKATNPSFCFNVLKSVGTTD 60
           MA +S LV+ SL+  L  LF N V S       D  STIC K +NP FC +VLKS GTT 
Sbjct: 1   MANNSCLVIVSLIGVL--LFTNNVTS-----STDVVSTICPKTSNPPFCSSVLKSAGTTH 60

Query: 61  LKGLAIFTLNLAHDKTIGNRALAQSLASKVADPELKDRYDTCVERYNDAVKDIEGAKGYL 120
           LKGLA++TLN AH     +  LA SLA    +P+L  RY +C E Y++A+ DIE A+  L
Sbjct: 61  LKGLAVYTLNFAHTNARKSLTLASSLAKTTLNPQLMQRYSSCAESYDEAIGDIENAQKDL 120

Query: 121 GKGDYKGVYTYTFGAMTEVDACLFRLSKLPKDSSALPGNGKAVEDIYSIILVIANLL 178
             GD+ GV   T GAMT +D C  +L++LPKD+S L  NGK + DI SIILVI+NLL
Sbjct: 121 ALGDFNGVNIVTSGAMTGIDDCQDKLAQLPKDTSLLLKNGKTLNDICSIILVISNLL 170

[Close]

BLAST of CmaCh17G012680.1 vs. NCBI nr
Match: gi|659127944|ref|XP_008463970.1| (PREDICTED: pectinesterase inhibitor-like [Cucumis melo])

HSP 1 Score: 156.4 bits (394), Expect = 4.8e-35
Identity = 86/177 (48.59%), Postives = 114/177 (64.41%), Query Frame = 1


Query: 1   MAYSSFLVVSSLLAFLSLLFFNGVVSIHAASPDDATSTICKKATNPSFCFNVLKSVGTTD 60
           M  +S +V+ SL+  +     + V     AS +D  S IC K +NP FC +VLKS GTTD
Sbjct: 1   MTNNSCIVIVSLIGVILFTIISNV-----ASSNDVISIICPKTSNPPFCSSVLKSAGTTD 60

Query: 61  LKGLAIFTLNLAHDKTIGNRALAQSLASKVADPELKDRYDTCVERYNDAVKDIEGAKGYL 120
           LKGLA++TLNLAH     +  LA+SLA+   +P+LK RY +C E Y++AV DIE A+  L
Sbjct: 61  LKGLAVYTLNLAHTNACKSLTLAKSLATTTTNPQLKQRYSSCFESYDEAVGDIENAQKDL 120

Query: 121 GKGDYKGVYTYTFGAMTEVDACLFRLSKLPKDSSALPGNGKAVEDIYSIILVIANLL 178
             GD+  V   T GAMTE+D C  + ++ PKD+S L  NGK + DI SIILVI+NLL
Sbjct: 121 ALGDFNAVNIVTSGAMTEIDDCQDKFAQPPKDTSLLLKNGKTLNDICSIILVISNLL 172

The following BLAST results are available for this feature:

BLAST of CmaCh17G012680.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PMEI_ACTDE	2.6e-17	34.64	Pectinesterase inhibitor OS=Actinidia deliciosa GN=PMEI PE=1 SV=2	[more]
PMEI1_ARATH	2.5e-15	32.12	Pectinesterase inhibitor 1 OS=Arabidopsis thaliana GN=PMEI1 PE=1 SV=1	[more]
PMEI2_ARATH	4.0e-13	32.93	Pectinesterase inhibitor 2 OS=Arabidopsis thaliana GN=PMEI2 PE=1 SV=1	[more]

BLAST of CmaCh17G012680.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KCV8_CUCSA	8.2e-34	47.73	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G307340 PE=4 SV=1	[more]
A0A0A0KEW6_CUCSA	2.7e-29	46.43	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G307350 PE=4 SV=1	[more]
A0A0A0KHY0_CUCSA	4.2e-22	41.01	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G307370 PE=4 SV=1	[more]
A0A0A0KCC4_CUCSA	1.4e-20	39.33	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G307360 PE=4 SV=1	[more]
G7JFU8_MEDTR	5.0e-15	36.99	Pectin methylesterase inhibitor OS=Medicago truncatula GN=MTR_4g025010 PE=4 SV=1	[more]

BLAST of CmaCh17G012680.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G48020.1	1.4e-16	32.12	pectin methylesterase inhibitor 1	[more]
AT3G17220.1	2.2e-14	32.93	pectin methylesterase inhibitor 2	[more]

BLAST of CmaCh17G012680.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659128306\|ref\|XP_008464144.1\|	2.3e-37	50.85	PREDICTED: pectinesterase inhibitor-like [Cucumis melo]	[more]
gi\|659113905\|ref\|XP_008456811.1\|	1.5e-36	49.72	PREDICTED: pectinesterase inhibitor-like [Cucumis melo]	[more]
gi\|659117593\|ref\|XP_008458683.1\|	4.3e-36	50.28	PREDICTED: pectinesterase inhibitor [Cucumis melo]	[more]
gi\|659131349\|ref\|XP_008465639.1\|	3.7e-35	50.85	PREDICTED: pectinesterase inhibitor-like [Cucumis melo]	[more]
gi\|659127944\|ref\|XP_008463970.1\|	4.8e-35	48.59	PREDICTED: pectinesterase inhibitor-like [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR006501	Pectinesterase_inhib_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0004857	enzyme inhibitor activity

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004857	enzyme inhibitor activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh17G012680	CmaCh17G012680	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh17G012680.1	CmaCh17G012680.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh17G012680.1.CDS.1	CmaCh17G012680.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh17G012680.1.exon.1	CmaCh17G012680.1.exon.1	exon

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	GENE3D	G3DSA:1.20.140.40		coord: 36..178 score: 3.9
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 37..172 score: 1.2
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 31..173 score: 4.2
IPR006501	Pectinesterase inhibitor domain	TIGRFAMs	TIGR01614	TIGR01614	coord: 9..177 score: 2.2
IPR006501	Pectinesterase inhibitor domain	unknown	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 37..178 score: 3.92
None	No IPR available	PANTHER	PTHR31707	FAMILY NOT NAMED	coord: 5..177 score: 2.4