CmaCh12G002700.1 (mRNA) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh12G002700.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Calcium ion binding protein
Location	Cma_Chr12 : 1396758 .. 1397383 (+)
Sequence length	513

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTATCTCCAGATTCATTGGGGACTTTTTACAAACAGTTGGTGGGCGATCCCGTGCGGCGGATTCCGCCGCTGACGGCGGTGCTCCGAGTCCCTGCAGAGTCATCCACGACGACGTGCTGTTAAGGGCGTTGATTACAGTGTTTGGAATGAGGGAAAATGGGAGAATCAAGAAAGAAAAAGCAAAGGGGGTGGTGGAGAAATTGGGGCTGATAAAGAGTGAAGAAGAGAAGGAAGGGTTTGAGCTGCCGGCGGATGGCGGTGGTGATGAGGTGGCGGTGGAGGAGGTGATCGGAGAGATGGAGGAGGAAGGGAAACGAAATGAGCTGCTATGGGAAGCTTTTAAGATATTTGATGTGGATGGCGATGGATTCATTGATACGGTTGAGCTGAAAAGAGTGCTTGAATGTTTGGGATTGGATAAAGGGTGGGGGATTAGGGAGATTGAGAAAATGGTTAGTGTTGTGGATGTGAATTTGGATGGGAAAGTTGATTTTGGTGAGTTTGAATTAATGATGGGTGTGAAATGTTCTTGATTTGTTCTTGTTTTTTTCACTTTTCAATCCAAAATTCTGGAAAAATCTTGTGATTTTGGTGTTATCTTTGTTCAGAAACCAAAACTTGA

mRNA sequence

ATGGGTATCTCCAGATTCATTGGGGACTTTTTACAAACAGTTGGTGGGCGATCCCGTGCGGCGGATTCCGCCGCTGACGGCGGTGCTCCGAGTCCCTGCAGAGTCATCCACGACGACGTGCTGTTAAGGGCGTTGATTACAGTGTTTGGAATGAGGGAAAATGGGAGAATCAAGAAAGAAAAAGCAAAGGGGGTGGTGGAGAAATTGGGGCTGATAAAGAGTGAAGAAGAGAAGGAAGGGTTTGAGCTGCCGGCGGATGGCGGTGGTGATGAGGTGGCGGTGGAGGAGGTGATCGGAGAGATGGAGGAGGAAGGGAAACGAAATGAGCTGCTATGGGAAGCTTTTAAGATATTTGATGTGGATGGCGATGGATTCATTGATACGGTTGAGCTGAAAAGAGTGCTTGAATGTTTGGGATTGGATAAAGGGTGGGGGATTAGGGAGATTGAGAAAATGGTTAGTGTTGTGGATGTGAATTTGGATGGGAAAGTTGATTTTGAAACCAAAACTTGA

Coding sequence (CDS)

ATGGGTATCTCCAGATTCATTGGGGACTTTTTACAAACAGTTGGTGGGCGATCCCGTGCGGCGGATTCCGCCGCTGACGGCGGTGCTCCGAGTCCCTGCAGAGTCATCCACGACGACGTGCTGTTAAGGGCGTTGATTACAGTGTTTGGAATGAGGGAAAATGGGAGAATCAAGAAAGAAAAAGCAAAGGGGGTGGTGGAGAAATTGGGGCTGATAAAGAGTGAAGAAGAGAAGGAAGGGTTTGAGCTGCCGGCGGATGGCGGTGGTGATGAGGTGGCGGTGGAGGAGGTGATCGGAGAGATGGAGGAGGAAGGGAAACGAAATGAGCTGCTATGGGAAGCTTTTAAGATATTTGATGTGGATGGCGATGGATTCATTGATACGGTTGAGCTGAAAAGAGTGCTTGAATGTTTGGGATTGGATAAAGGGTGGGGGATTAGGGAGATTGAGAAAATGGTTAGTGTTGTGGATGTGAATTTGGATGGGAAAGTTGATTTTGAAACCAAAACTTGA

Protein sequence

MGISRFIGDFLQTVGGRSRAADSAADGGAPSPCRVIHDDVLLRALITVFGMRENGRIKKEKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEEGKRNELLWEAFKIFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFETKT

Homology

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BLAST of CmaCh12G002700.1 vs. Swiss-Prot
Match: CML2_ARATH (Calmodulin-like protein 2 OS=Arabidopsis thaliana GN=CML2 PE=2 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.2e-11
Identity = 45/130 (34.62%), Postives = 70/130 (53.85%), Query Frame = 1


Query: 42  LRALITVFGMRENGRIKKEKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIG-- 101
           L  +  +F    +G+I K + K   + +G++  E E        D  GD     +  G  
Sbjct: 6   LSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGSL 65

Query: 102 --EMEEEGKRNELLWEAFKIFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVD 161
             EM EE +  E + EAF++FD +GDGFI   EL+ VL  +GL +G  + + +KM+S VD
Sbjct: 66  YQEMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVD 125

Query: 162 VNLDGKVDFE 168
           V+ DG V+F+
Sbjct: 126 VDGDGMVNFK 135

[Close]

BLAST of CmaCh12G002700.1 vs. Swiss-Prot
Match: CML7_ARATH (Calmodulin-like protein 7 OS=Arabidopsis thaliana GN=CML7 PE=2 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.7e-10
Identity = 44/135 (32.59%), Postives = 70/135 (51.85%), Query Frame = 1


Query: 38  DDVLLRALITVFGMRENGRIKKEKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEV 97
           D   L+ +  +F    +G I  ++    +  LG+   ++E        D  GD     + 
Sbjct: 2   DPTELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDE 61

Query: 98  IGE-----MEEEGKRNELLWEAFKIFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKM 157
            GE     M+EE +  E + EAF +FD +GDGFI   ELK VL  LGL +G  + + +KM
Sbjct: 62  FGELYKTIMDEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGKTLDDCKKM 121

Query: 158 VSVVDVNLDGKVDFE 168
           +  VDV+ DG+V+++
Sbjct: 122 IKKVDVDGDGRVNYK 136

[Close]

BLAST of CmaCh12G002700.1 vs. Swiss-Prot
Match: CML43_ARATH (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana GN=CML43 PE=2 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.7e-10
Identity = 33/63 (52.38%), Postives = 43/63 (68.25%), Query Frame = 1


Query: 104 EGKRNELLWEAFKIFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGK 163
           +G     L EAF +FD DGDGFI  VEL++VL+ LGL +   I ++EKM+  VD N DG+
Sbjct: 105 DGSPESDLEEAFNVFDEDGDGFISAVELQKVLKKLGLPEAGEIEQVEKMIVSVDSNHDGR 164

Query: 164 VDF 167
           VDF
Sbjct: 165 VDF 167

[Close]

BLAST of CmaCh12G002700.1 vs. Swiss-Prot
Match: CML6_ARATH (Calmodulin-like protein 6 OS=Arabidopsis thaliana GN=CML6 PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 2.3e-10
Identity = 46/136 (33.82%), Postives = 68/136 (50.00%), Query Frame = 1


Query: 38  DDVLLRALITVFGMRENGRIKKEKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEV 97
           D   L  +  +F    +G+I  ++     + LG+I  E+E        D  GD     E 
Sbjct: 2   DSTELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEE 61

Query: 98  IGEM-------EEEGKRNELLWEAFKIFDVDGDGFIDTVELKRVLECLGLDKGWGIREIE 157
            GE+       +E+    E + EAF +FD +GDGFI   ELK VL  LGL +G  + E  
Sbjct: 62  FGELYKTIMVEDEDEVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKTLEECR 121

Query: 158 KMVSVVDVNLDGKVDF 167
           KM+  VDV+ DG+V++
Sbjct: 122 KMIMQVDVDGDGRVNY 137

[Close]

BLAST of CmaCh12G002700.1 vs. Swiss-Prot
Match: CML5_ARATH (Calmodulin-like protein 5 OS=Arabidopsis thaliana GN=CML5 PE=2 SV=2)

HSP 1 Score: 65.1 bits (157), Expect = 8.6e-10
Identity = 42/135 (31.11%), Postives = 69/135 (51.11%), Query Frame = 1


Query: 42  LRALITVFGMRENGRIKKEKAKGVVEKLGLIKSEEEKEGFELPADGGGD--------EVA 101
           L+ +  +F    +GRI KE+    +E LG+   +++        D  GD        E  
Sbjct: 66  LKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDIDEFESL 125

Query: 102 VEEVIGEMEEEGKRNEL-LWEAFKIFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKM 161
              ++ E   +G+  E  + +AF +FD DGDGFI   ELK V+  LGL +G  +   +KM
Sbjct: 126 YSSIVDEHHNDGETEEEDMKDAFNVFDQDGDGFITVEELKSVMASLGLKQGKTLDGCKKM 185

Query: 162 VSVVDVNLDGKVDFE 168
           +  VD + DG+V+++
Sbjct: 186 IMQVDADGDGRVNYK 200

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BLAST of CmaCh12G002700.1 vs. TrEMBL
Match: A0A0A0LP90_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G376820 PE=4 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 1.5e-66
Identity = 139/167 (83.23%), Postives = 149/167 (89.22%), Query Frame = 1


Query: 1   MGISRFIGDFLQTVGGRSRAA-DSAADGGAPSPCRVIHDDVLLRALITVFGMRENGRIKK 60
           MGISRFIGDFLQT+GGRSRAA +S  D G   P R+++DDVLLRALITVFGMRENGRIK 
Sbjct: 1   MGISRFIGDFLQTLGGRSRAAVESTIDAGESKPNRMVYDDVLLRALITVFGMRENGRIKT 60

Query: 61  EKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEEGKRNELLWEAFKIFD 120
           EKAKGVVEKLGLI   EEKE FEL A  GGDEVAVEE+IGE  EEGKRNELL+EAFKIFD
Sbjct: 61  EKAKGVVEKLGLI---EEKEKFELAAGEGGDEVAVEEMIGE--EEGKRNELLYEAFKIFD 120

Query: 121 VDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDF 167
           VDGDGFIDT+ELKRV++CLGLDKGWGIREIEKMVSVVDVNLDGKVDF
Sbjct: 121 VDGDGFIDTIELKRVIDCLGLDKGWGIREIEKMVSVVDVNLDGKVDF 162

[Close]

BLAST of CmaCh12G002700.1 vs. TrEMBL
Match: M5Y644_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa015775mg PE=4 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 4.5e-42
Identity = 103/168 (61.31%), Postives = 118/168 (70.24%), Query Frame = 1


Query: 4   SRFIGDFLQTVGGRSR-AADSAADGGAPSPCRVIH-DDVLLRALITVFGMRENGRIKKEK 63
           SR +GD +Q  GG SR + +     G+  P      D+ + RALITVFGM  NGRIKKE 
Sbjct: 8   SRLLGDLVQAFGGVSRPSTECKPQNGSCKPQNASKVDESMARALITVFGMETNGRIKKEN 67

Query: 64  AKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEE---GKRNELLWEAFKIF 123
           A+ VVE LGLI  EE+K  F+LP D    EV VEEV+G +EEE    KRNELL EAFKIF
Sbjct: 68  ARRVVENLGLICHEEDKSSFDLPGDD--HEVPVEEVLGGLEEEDDGSKRNELLLEAFKIF 127

Query: 124 DVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDF 167
           D DGDGFI+ VELKRVLECLGL  GW + +IEKMV VVDVNLDGKVDF
Sbjct: 128 DEDGDGFIEAVELKRVLECLGLGSGWDMDQIEKMVRVVDVNLDGKVDF 173

[Close]

BLAST of CmaCh12G002700.1 vs. TrEMBL
Match: I3T705_LOTJA (Uncharacterized protein OS=Lotus japonicus PE=2 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 8.5e-41
Identity = 100/171 (58.48%), Postives = 122/171 (71.35%), Query Frame = 1


Query: 4   SRFIGDFLQTVGGRSRAADSAADGGAPSPCRVIH-DDVLLRALITVFGMRENGRIKKEKA 63
           SRFIGDF +T+     ++ S  D        +I   + ++RAL+TVFGM  NGRIKKE A
Sbjct: 8   SRFIGDFFKTLKSSPPSSISHLDQPTCKAQDMIKLGEKMVRALVTVFGMENNGRIKKENA 67

Query: 64  KGVVEKLGLI--KSEEEKEG---FELPADGG--GDEVAVEEVIGEMEEEGKRNELLWEAF 123
           + VVEKLGL+   SEE+K     FELP +GG   DEV VEEV+GE+E+  KR+ELL EAF
Sbjct: 68  RKVVEKLGLMYGNSEEDKNKVSEFELPCEGGFHDDEVPVEEVLGELEDMSKRSELLREAF 127

Query: 124 KIFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDF 167
           KIFD DGDG+ID +ELKRVL+CLGLDKGW +  IE MV VVD+N DGKVDF
Sbjct: 128 KIFDEDGDGYIDALELKRVLDCLGLDKGWDMNTIESMVKVVDLNFDGKVDF 178

[Close]

BLAST of CmaCh12G002700.1 vs. TrEMBL
Match: A0A067FM67_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g039672mg PE=4 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 9.4e-40
Identity = 100/177 (56.50%), Postives = 123/177 (69.49%), Query Frame = 1


Query: 4   SRFIGDFLQTVGGRSRAADSAADGGAPSPCRVIH---------DDVLLRALITVFGMREN 63
           SR IGD +Q +G  SR   S++     +  R            DD++LRAL  VFGM +N
Sbjct: 9   SRLIGDLVQAIGV-SRPRSSSSHNIVTNSTRECEQQTETAGPVDDLMLRALRAVFGMEKN 68

Query: 64  GRIKKEKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIG----EMEEEG-KRNE 123
           G+IKKE+AK VVEKLGLI +E+EK  F+LP  G  DEV VEE +G    E++ EG  R+E
Sbjct: 69  GKIKKERAKKVVEKLGLIYNEDEKSSFDLPGTGLEDEVPVEEALGLGLGELDGEGCGRDE 128

Query: 124 LLWEAFKIFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDF 167
           LL +AFKIFD DG+G+ID  ELKRVLECLGLDKGW + EIEKM+ VVD+NLDGKVDF
Sbjct: 129 LLRKAFKIFDEDGNGYIDASELKRVLECLGLDKGWDMGEIEKMLKVVDLNLDGKVDF 184

[Close]

BLAST of CmaCh12G002700.1 vs. TrEMBL
Match: V4WAJ0_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10010458mg PE=4 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 9.4e-40
Identity = 100/177 (56.50%), Postives = 123/177 (69.49%), Query Frame = 1


Query: 4   SRFIGDFLQTVGGRSRAADSAADGGAPSPCRVIH---------DDVLLRALITVFGMREN 63
           SR IGD +Q +G  SR   S++     +  R            DD++LRAL  VFGM +N
Sbjct: 9   SRLIGDLVQAIGV-SRPRSSSSHNIVTNSTRECEQQTETAGPVDDLMLRALRAVFGMEKN 68

Query: 64  GRIKKEKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIG----EMEEEG-KRNE 123
           G+IKKE+AK VVEKLGLI +E+EK  F+LP  G  DEV VEE +G    E++ EG  R+E
Sbjct: 69  GKIKKERAKKVVEKLGLIYNEDEKSSFDLPGTGLEDEVPVEEALGLGLGELDGEGCGRDE 128

Query: 124 LLWEAFKIFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDF 167
           LL +AFKIFD DG+G+ID  ELKRVLECLGLDKGW + EIEKM+ VVD+NLDGKVDF
Sbjct: 129 LLRKAFKIFDEDGNGYIDASELKRVLECLGLDKGWDMGEIEKMLKVVDLNLDGKVDF 184

[Close]

BLAST of CmaCh12G002700.1 vs. TAIR10
Match: AT4G12860.1 (AT4G12860.1 EF hand calcium-binding protein family)

HSP 1 Score: 71.2 bits (173), Expect = 6.7e-13
Identity = 45/130 (34.62%), Postives = 70/130 (53.85%), Query Frame = 1


Query: 42  LRALITVFGMRENGRIKKEKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIG-- 101
           L  +  +F    +G+I K + K   + +G++  E E        D  GD     +  G  
Sbjct: 6   LSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGSL 65

Query: 102 --EMEEEGKRNELLWEAFKIFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVD 161
             EM EE +  E + EAF++FD +GDGFI   EL+ VL  +GL +G  + + +KM+S VD
Sbjct: 66  YQEMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVD 125

Query: 162 VNLDGKVDFE 168
           V+ DG V+F+
Sbjct: 126 VDGDGMVNFK 135

[Close]

BLAST of CmaCh12G002700.1 vs. TAIR10
Match: AT1G05990.1 (AT1G05990.1 EF hand calcium-binding protein family)

HSP 1 Score: 67.4 bits (163), Expect = 9.7e-12
Identity = 44/135 (32.59%), Postives = 70/135 (51.85%), Query Frame = 1


Query: 38  DDVLLRALITVFGMRENGRIKKEKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEV 97
           D   L+ +  +F    +G I  ++    +  LG+   ++E        D  GD     + 
Sbjct: 2   DPTELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDE 61

Query: 98  IGE-----MEEEGKRNELLWEAFKIFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKM 157
            GE     M+EE +  E + EAF +FD +GDGFI   ELK VL  LGL +G  + + +KM
Sbjct: 62  FGELYKTIMDEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGKTLDDCKKM 121

Query: 158 VSVVDVNLDGKVDFE 168
           +  VDV+ DG+V+++
Sbjct: 122 IKKVDVDGDGRVNYK 136

[Close]

BLAST of CmaCh12G002700.1 vs. TAIR10
Match: AT5G44460.1 (AT5G44460.1 calmodulin like 43)

HSP 1 Score: 67.4 bits (163), Expect = 9.7e-12
Identity = 33/63 (52.38%), Postives = 43/63 (68.25%), Query Frame = 1


Query: 104 EGKRNELLWEAFKIFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGK 163
           +G     L EAF +FD DGDGFI  VEL++VL+ LGL +   I ++EKM+  VD N DG+
Sbjct: 105 DGSPESDLEEAFNVFDEDGDGFISAVELQKVLKKLGLPEAGEIEQVEKMIVSVDSNHDGR 164

Query: 164 VDF 167
           VDF
Sbjct: 165 VDF 167

[Close]

BLAST of CmaCh12G002700.1 vs. TAIR10
Match: AT4G03290.1 (AT4G03290.1 EF hand calcium-binding protein family)

HSP 1 Score: 67.0 bits (162), Expect = 1.3e-11
Identity = 46/136 (33.82%), Postives = 68/136 (50.00%), Query Frame = 1


Query: 38  DDVLLRALITVFGMRENGRIKKEKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEV 97
           D   L  +  +F    +G+I  ++     + LG+I  E+E        D  GD     E 
Sbjct: 2   DSTELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEE 61

Query: 98  IGEM-------EEEGKRNELLWEAFKIFDVDGDGFIDTVELKRVLECLGLDKGWGIREIE 157
            GE+       +E+    E + EAF +FD +GDGFI   ELK VL  LGL +G  + E  
Sbjct: 62  FGELYKTIMVEDEDEVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKTLEECR 121

Query: 158 KMVSVVDVNLDGKVDF 167
           KM+  VDV+ DG+V++
Sbjct: 122 KMIMQVDVDGDGRVNY 137

[Close]

BLAST of CmaCh12G002700.1 vs. TAIR10
Match: AT2G43290.1 (AT2G43290.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 65.1 bits (157), Expect = 4.8e-11
Identity = 42/135 (31.11%), Postives = 69/135 (51.11%), Query Frame = 1


Query: 42  LRALITVFGMRENGRIKKEKAKGVVEKLGLIKSEEEKEGFELPADGGGD--------EVA 101
           L+ +  +F    +GRI KE+    +E LG+   +++        D  GD        E  
Sbjct: 66  LKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDIDEFESL 125

Query: 102 VEEVIGEMEEEGKRNEL-LWEAFKIFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKM 161
              ++ E   +G+  E  + +AF +FD DGDGFI   ELK V+  LGL +G  +   +KM
Sbjct: 126 YSSIVDEHHNDGETEEEDMKDAFNVFDQDGDGFITVEELKSVMASLGLKQGKTLDGCKKM 185

Query: 162 VSVVDVNLDGKVDFE 168
           +  VD + DG+V+++
Sbjct: 186 IMQVDADGDGRVNYK 200

[Close]

BLAST of CmaCh12G002700.1 vs. NCBI nr
Match: gi|659088575|ref|XP_008445056.1| (PREDICTED: calmodulin-like protein 7 [Cucumis melo])

HSP 1 Score: 261.5 bits (667), Expect = 1.0e-66
Identity = 140/167 (83.83%), Postives = 149/167 (89.22%), Query Frame = 1


Query: 1   MGISRFIGDFLQTVGGRSRAA-DSAADGGAPSPCRVIHDDVLLRALITVFGMRENGRIKK 60
           MGISRFIGDFLQT+GGRSRAA +S  D G   P R+++DDVLLRALITVFGMRENGRIK 
Sbjct: 1   MGISRFIGDFLQTLGGRSRAAAESTIDAGESKPNRMVYDDVLLRALITVFGMRENGRIKT 60

Query: 61  EKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEEGKRNELLWEAFKIFD 120
           EKAKGVVEKLGLI   EEKE FEL A  GGDEVAVEEVIGE  EEGKRNELL+EAFKIFD
Sbjct: 61  EKAKGVVEKLGLI---EEKEKFELAAGEGGDEVAVEEVIGE--EEGKRNELLYEAFKIFD 120

Query: 121 VDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDF 167
           VDGDGFIDT+ELKRV++CLGLDKGWGIREIEKMVSVVDVNLDGKVDF
Sbjct: 121 VDGDGFIDTIELKRVIDCLGLDKGWGIREIEKMVSVVDVNLDGKVDF 162

[Close]

BLAST of CmaCh12G002700.1 vs. NCBI nr
Match: gi|449442228|ref|XP_004138884.1| (PREDICTED: calmodulin-like protein 2 [Cucumis sativus])

HSP 1 Score: 260.4 bits (664), Expect = 2.2e-66
Identity = 139/167 (83.23%), Postives = 149/167 (89.22%), Query Frame = 1


Query: 1   MGISRFIGDFLQTVGGRSRAA-DSAADGGAPSPCRVIHDDVLLRALITVFGMRENGRIKK 60
           MGISRFIGDFLQT+GGRSRAA +S  D G   P R+++DDVLLRALITVFGMRENGRIK 
Sbjct: 1   MGISRFIGDFLQTLGGRSRAAVESTIDAGESKPNRMVYDDVLLRALITVFGMRENGRIKT 60

Query: 61  EKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEEGKRNELLWEAFKIFD 120
           EKAKGVVEKLGLI   EEKE FEL A  GGDEVAVEE+IGE  EEGKRNELL+EAFKIFD
Sbjct: 61  EKAKGVVEKLGLI---EEKEKFELAAGEGGDEVAVEEMIGE--EEGKRNELLYEAFKIFD 120

Query: 121 VDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDF 167
           VDGDGFIDT+ELKRV++CLGLDKGWGIREIEKMVSVVDVNLDGKVDF
Sbjct: 121 VDGDGFIDTIELKRVIDCLGLDKGWGIREIEKMVSVVDVNLDGKVDF 162

[Close]

BLAST of CmaCh12G002700.1 vs. NCBI nr
Match: gi|645226377|ref|XP_008220010.1| (PREDICTED: probable calcium-binding protein CML43 [Prunus mume])

HSP 1 Score: 179.1 bits (453), Expect = 6.5e-42
Identity = 103/168 (61.31%), Postives = 118/168 (70.24%), Query Frame = 1


Query: 4   SRFIGDFLQTVGGRSR-AADSAADGGAPSPCRVIH-DDVLLRALITVFGMRENGRIKKEK 63
           SR +GD +Q  GG SR + +     G+  P      D+ + RALITVFGM  NGRIKKE 
Sbjct: 8   SRLLGDLVQAFGGVSRPSTECKPQNGSCKPQNASKVDESMARALITVFGMETNGRIKKEN 67

Query: 64  AKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEE---GKRNELLWEAFKIF 123
           A+ VVE LGLI  EE+K  F+LP D    EV VEEV+G +EEE    KRNELL EAFKIF
Sbjct: 68  ARRVVENLGLICHEEDKSSFDLPGDD--HEVPVEEVLGGLEEEDDGSKRNELLLEAFKIF 127

Query: 124 DVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDF 167
           D DGDGFI+ VELKRVLECLGL  GW + +IEKMV VVDVNLDGKVDF
Sbjct: 128 DEDGDGFIEAVELKRVLECLGLGSGWDMDQIEKMVRVVDVNLDGKVDF 173

[Close]

BLAST of CmaCh12G002700.1 vs. NCBI nr
Match: gi|596290461|ref|XP_007226186.1| (hypothetical protein PRUPE_ppa015775mg [Prunus persica])

HSP 1 Score: 179.1 bits (453), Expect = 6.5e-42
Identity = 103/168 (61.31%), Postives = 118/168 (70.24%), Query Frame = 1


Query: 4   SRFIGDFLQTVGGRSR-AADSAADGGAPSPCRVIH-DDVLLRALITVFGMRENGRIKKEK 63
           SR +GD +Q  GG SR + +     G+  P      D+ + RALITVFGM  NGRIKKE 
Sbjct: 8   SRLLGDLVQAFGGVSRPSTECKPQNGSCKPQNASKVDESMARALITVFGMETNGRIKKEN 67

Query: 64  AKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEE---GKRNELLWEAFKIF 123
           A+ VVE LGLI  EE+K  F+LP D    EV VEEV+G +EEE    KRNELL EAFKIF
Sbjct: 68  ARRVVENLGLICHEEDKSSFDLPGDD--HEVPVEEVLGGLEEEDDGSKRNELLLEAFKIF 127

Query: 124 DVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDF 167
           D DGDGFI+ VELKRVLECLGL  GW + +IEKMV VVDVNLDGKVDF
Sbjct: 128 DEDGDGFIEAVELKRVLECLGLGSGWDMDQIEKMVRVVDVNLDGKVDF 173

[Close]

BLAST of CmaCh12G002700.1 vs. NCBI nr
Match: gi|1021576253|ref|XP_016179334.1| (PREDICTED: probable calcium-binding protein CML32 [Arachis ipaensis])

HSP 1 Score: 174.9 bits (442), Expect = 1.2e-40
Identity = 104/180 (57.78%), Postives = 122/180 (67.78%), Query Frame = 1


Query: 4   SRFIGDFLQTVGGRSRAAD--SAADGGAPSP-CRVIH----DDVLLRALITVFGMRENGR 63
           SRFI DFLQ     S  +      D    SP C+  +    DD  +RAL+TVFGM+ NGR
Sbjct: 8   SRFIVDFLQAFKHSSPPSSIHDELDHNHHSPDCKPQNSLKLDDNTVRALMTVFGMQNNGR 67

Query: 64  IKKEKAKGVVEKLGLI-------KSEEEKEGFELPADGG---GDEVAVEEVIGEMEEEGK 123
           IKKE A+ VVEKLGL+       K+      FEL  DGG   GDEV VEEV+G++E+  K
Sbjct: 68  IKKENARRVVEKLGLVHDKDNNNKASSSPSRFELHGDGGLLEGDEVPVEEVLGDLEDMSK 127

Query: 124 RNELLWEAFKIFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDF 167
           RNELL EAFKIFD DGDG+ID +ELKRVL+CLGLDKGW +  IEKMV VVD+NLDGKVDF
Sbjct: 128 RNELLHEAFKIFDEDGDGYIDAMELKRVLDCLGLDKGWDMNTIEKMVKVVDLNLDGKVDF 187

The following BLAST results are available for this feature:

BLAST of CmaCh12G002700.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CML2_ARATH	1.2e-11	34.62	Calmodulin-like protein 2 OS=Arabidopsis thaliana GN=CML2 PE=2 SV=1	[more]
CML7_ARATH	1.7e-10	32.59	Calmodulin-like protein 7 OS=Arabidopsis thaliana GN=CML7 PE=2 SV=1	[more]
CML43_ARATH	1.7e-10	52.38	Probable calcium-binding protein CML43 OS=Arabidopsis thaliana GN=CML43 PE=2 SV=...	[more]
CML6_ARATH	2.3e-10	33.82	Calmodulin-like protein 6 OS=Arabidopsis thaliana GN=CML6 PE=2 SV=1	[more]
CML5_ARATH	8.6e-10	31.11	Calmodulin-like protein 5 OS=Arabidopsis thaliana GN=CML5 PE=2 SV=2	[more]

BLAST of CmaCh12G002700.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LP90_CUCSA	1.5e-66	83.23	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G376820 PE=4 SV=1	[more]
M5Y644_PRUPE	4.5e-42	61.31	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa015775mg PE=4 SV=1	[more]
I3T705_LOTJA	8.5e-41	58.48	Uncharacterized protein OS=Lotus japonicus PE=2 SV=1	[more]
A0A067FM67_CITSI	9.4e-40	56.50	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g039672mg PE=4 SV=1	[more]
V4WAJ0_9ROSI	9.4e-40	56.50	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10010458mg PE=4 SV=1	[more]

BLAST of CmaCh12G002700.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G12860.1	6.7e-13	34.62	EF hand calcium-binding protein family	[more]
AT1G05990.1	9.7e-12	32.59	EF hand calcium-binding protein family	[more]
AT5G44460.1	9.7e-12	52.38	calmodulin like 43	[more]
AT4G03290.1	1.3e-11	33.82	EF hand calcium-binding protein family	[more]
AT2G43290.1	4.8e-11	31.11	Calcium-binding EF-hand family protein	[more]

BLAST of CmaCh12G002700.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659088575\|ref\|XP_008445056.1\|	1.0e-66	83.83	PREDICTED: calmodulin-like protein 7 [Cucumis melo]	[more]
gi\|449442228\|ref\|XP_004138884.1\|	2.2e-66	83.23	PREDICTED: calmodulin-like protein 2 [Cucumis sativus]	[more]
gi\|645226377\|ref\|XP_008220010.1\|	6.5e-42	61.31	PREDICTED: probable calcium-binding protein CML43 [Prunus mume]	[more]
gi\|596290461\|ref\|XP_007226186.1\|	6.5e-42	61.31	hypothetical protein PRUPE_ppa015775mg [Prunus persica]	[more]
gi\|1021576253\|ref\|XP_016179334.1\|	1.2e-40	57.78	PREDICTED: probable calcium-binding protein CML32 [Arachis ipaensis]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh12G002700	CmaCh12G002700	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh12G002700.1	CmaCh12G002700.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh12G002700.1.exon.1	CmaCh12G002700.1.exon.1	exon
CmaCh12G002700.1.exon.2	CmaCh12G002700.1.exon.2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh12G002700.1.CDS.1	CmaCh12G002700.1.CDS.1	CDS
CmaCh12G002700.1.CDS.2	CmaCh12G002700.1.CDS.2	CDS

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 111..139 score: 5.
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 110..138 score: 0.
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 144..170 score: 6.145coord: 106..141 score: 1
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 107..167 score: 2.1
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 48..167 score: 1.73
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 119..131 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 53..167 score: 7.3
None	No IPR available	PANTHER	PTHR10891:SF574	CALCIUM-BINDING PROTEIN CML30-RELATED	coord: 53..167 score: 7.3