CmaCh12G002520.1 (mRNA) Cucurbita maxima (Rimu)

NameCmaCh12G002520.1
TypemRNA
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionCarotenoid cleavage dioxygenase 8
LocationCma_Chr12 : 1265328 .. 1268282 (-)
Sequence length1557
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCATGGCTGCCGGCAGCTGCATGTCTTCCTTCAACTTCCAATCCATCCACCCTCCAAAACGCACCGTTTCCAACTTCACCATTAGAAATATAGCCACCCCCATCCGTCCTCCATCTGTCGTTGTTCCATTGCCCGAGATCGACACTCCTGCCCACCCGAATCACGTTGCATGGACTAGCGTGCAACAAGAGCAATGGGAAGGAGAACTCTCCGTTCAAGGCCAATTGCCCCGTTGGCTGGTTCGTTTTGTTATTATTAATGTTTTAATCGTTGAACTATGTTTAGGTTTGATCTTAGATAAAACAAGTTGTCCAGAGTCTCTCTTGCGTGGACGCGATTGTTTATGGTTACATGTTAGAATTCTAATAGCTTATCAAACCTTTAATTAATTAATCGTAAGTATTGTTTACGCGTTTAATCTTCCAATAATTAATATTGATAATTTCGAAGTTATTTTGATAATTTTTTTAACTTGATGTATGACTCATGCAGAATGGGACGTACCTGAGAAATGGACCTGGAATATGGCATATTGGCGATTATAATTTTCGGCATCTCTTCGATGGCTATGCTACAATTGTTAAGCTACAGTTTGATGATGGTCGACTAATTGCAGGCCATCGCCAGATCGAATCAGATGCGTATAAAGCTGCTATGAAGAATCAAAAGATTTGTTATCGTGAATTCTCTGAAGTACCCAAGACTAATAACTTTCTTGCCTACGTTGGGGAGCTTGCAAGTCTTTTCTCTGGTGCATCATTGACTGATAATGCAAACACTGGGGTTGTGAAACTTGGCGATGGCCGAGTCATTTGCCTTACAGAAACTCAAAAAGGGTCAATAATGATCAACCCAACCACGTTAGAGACTGTGGGGAAGTTTGAGTATAGCGATTCACTTGGCGGTTTAATTCACTCAGCTCATCCAATTGTTACAGATTTTGAATTTCTTACATTGTTGCCAGATCTTTTGAACCCAGGTTATTTGGTGGTTCGTATGGAACCGGGCTCAAATGAGCGTAAGGTTATCAGTCGGGTTAACTGCAAGGGCGGACCAGCCCCAGGTTGGGTCCACTCGTTTCCGGTGACGGAGAATTACGTGGTGGTGCCGGAGATGCCATTGAGATATTGCGCTCAAAACTTGTTGAGGGCGGAGCCAACGCCGTTGTACAAGTTTGAGTGGCGGCCTGAGTCCAAAGCCTATGTGCATGTCATGTGCAAAGCTAGTGGAAAAATTGTAAGTTTATTTTTTTTCTATCCGTCTTGATGTCAAATTTAATACAAGTTTCTATAATGTTTTCAATGAAATCCAGGTAGCATCTGTTGAAGTACCATTATACATTACGTTTCATTTCATAAATGCATACGAGGAGAGGGACGAAAAGGGAAGGATTACAGCCGTGATAGCCGATTGTTGCGAACATAACGCTAACCCGCTAATCTTGGACCGACTTCGGCTTCATAATTTGCGTCAATTCACTCCCAACGATGCCCTTCCAGATGCTAGGTTTGTTTAATCTCCCTCTAATCTTAAAGGGTTTGGAATTGGGAAGGAGAGTGCCCCAATGGAGTGACTCGAGAATGTGTTTAGGTATGCATGTAGACAGGGGCATCAGTTTTTGTAACAACTTAGGTCCATCACTAGTAGATATTGTTTATATTTGGGTTTACCCTCAAGGATTTTAGAACGTGTCAGCTAGGGAGAAGCTTCCACACCCTTATAAAGAATGTTCTATTCTCCTCCCCAACTGATGTGGGATCTCACAATCCACCCCCTTTGGGGCCAATGTTCTCGCTGACACTTGTTCCCTTCTCCAATCAATGTGTGACCCCCCAATCCACCCCCTTCGAGACCCAACGTTCTTGTTGGTACACCACCTCGTGTCCACCCCTTTGGGGATCAGCCTCCCTGTTGGCACATCGCCCGGTGTCTAGCTCTAATACCATTTGTAACAGTCTAAGCCCACCGCTAACAGATATTGTCCTCTCTCAAGCTTCCCCTCAAGGTTTGTTAAAACGCGTCTGCTAGGAAAAGGTTTCCACACTTTTATAAAGAATGTTTCGTTTTCTTCTCAACCGATGTGGGATCTCACATTTCTACAACCCAATTTGTTGACTTGTTTGGAGAGGATCGATTACTGTTCATGTTGTTTTGCTTTTGCGGTCTGATTTGGTTCCATGGAGCTTTGATTTGTTTCGTGGGGTGGAAAATTCGAAGGTCTGGCTGTGCTCAGCTCAGATTGGCACGTCAAATGCTTAAATTCACTAATAGAAATGGAACTATGCAGGCTAGGAAGGTTCAGAATACCACTGGATGGGAGTGGATATGGGGAGCTGGAGGCAGCATTGGACCCAGACGAACATGGTAGAGGGTTAGACATGTGCAGCTTCAACCCTGCTTACTTGGGCAAGAAATATAGATATGCTTATGCCTGTGGAGCCCAAAGACCCTGCAATTTCCCCAACACCCTCACTAAGGCAACACTTTCATAACCATTTTCTCAATCTGAGCTTACTTTTGTTCTTCTTCCTCAATTCACTTTTTCCCTTCATTCATTTCAGATTGATTTGGTGAAGAAGAAGGCAAAGAATTGGCACGAGGCAGGTGCTATACCTTCCGAGCCCTTCTTCGTGGCTCGACCTGGTGCAACAAAAGAAGATGATGGTGAGTCGTTATATCATCATCCCTAAGTGAACAAAGCGAATAAACGTACAACCCGAAAGTTCTCGTATTTGTAATCGTCTTTGATTCAAACTAACGTAAGGCTTTGGTTGAACTGAAATGTGTTTATTTTGGATGCAGGGGTGGTGATCTCTATGGTCAGTGGGGAAGATGGAGAAGGGTATGCTTTGCTACTAGATGGATCCACATTTGAAGAGATAGCCAGAGCAAAATTTCCCTATGGTCTTCCTTATGGCCTACATGGTTGCTGGATTCCCAAGAATTAG

mRNA sequence

ATGGCTTCCATGGCTGCCGGCAGCTGCATGTCTTCCTTCAACTTCCAATCCATCCACCCTCCAAAACGCACCGTTTCCAACTTCACCATTAGAAATATAGCCACCCCCATCCGTCCTCCATCTGTCGTTGTTCCATTGCCCGAGATCGACACTCCTGCCCACCCGAATCACGTTGCATGGACTAGCGTGCAACAAGAGCAATGGGAAGGAGAACTCTCCGTTCAAGGCCAATTGCCCCGTTGGCTGAATGGGACGTACCTGAGAAATGGACCTGGAATATGGCATATTGGCGATTATAATTTTCGGCATCTCTTCGATGGCTATGCTACAATTGTTAAGCTACAGTTTGATGATGGTCGACTAATTGCAGGCCATCGCCAGATCGAATCAGATGCGTATAAAGCTGCTATGAAGAATCAAAAGATTTGTTATCGTGAATTCTCTGAAGTACCCAAGACTAATAACTTTCTTGCCTACGTTGGGGAGCTTGCAAGTCTTTTCTCTGGTGCATCATTGACTGATAATGCAAACACTGGGGTTGTGAAACTTGGCGATGGCCGAGTCATTTGCCTTACAGAAACTCAAAAAGGGTCAATAATGATCAACCCAACCACGTTAGAGACTGTGGGGAAGTTTGAGTATAGCGATTCACTTGGCGGTTTAATTCACTCAGCTCATCCAATTGTTACAGATTTTGAATTTCTTACATTGTTGCCAGATCTTTTGAACCCAGGTTATTTGGTGGTTCGTATGGAACCGGGCTCAAATGAGCGTAAGGTTATCAGTCGGGTTAACTGCAAGGGCGGACCAGCCCCAGGTTGGGTCCACTCGTTTCCGGTGACGGAGAATTACGTGGTGGTGCCGGAGATGCCATTGAGATATTGCGCTCAAAACTTGTTGAGGGCGGAGCCAACGCCGTTGTACAAGTTTGAGTGGCGGCCTGAGTCCAAAGCCTATGTGCATGTCATGTGCAAAGCTAGTGGAAAAATTGTAGCATCTGTTGAAGTACCATTATACATTACGTTTCATTTCATAAATGCATACGAGGAGAGGGACGAAAAGGGAAGGATTACAGCCGTGATAGCCGATTGTTGCGAACATAACGCTAACCCGCTAATCTTGGACCGACTTCGGCTTCATAATTTGCGTCAATTCACTCCCAACGATGCCCTTCCAGATGCTAGGCTAGGAAGGTTCAGAATACCACTGGATGGGAGTGGATATGGGGAGCTGGAGGCAGCATTGGACCCAGACGAACATGCTTCAACCCTGCTTACTTGGGCAAGAAATATAGATATGCTTATGCCTATTGATTTGGTGAAGAAGAAGGCAAAGAATTGGCACGAGGCAGGTGCTATACCTTCCGAGCCCTTCTTCGTGGCTCGACCTGGTGCAACAAAAGAAGATGATGGGGTGGTGATCTCTATGGTCAGTGGGGAAGATGGAGAAGGGTATGCTTTGCTACTAGATGGATCCACATTTGAAGAGATAGCCAGAGCAAAATTTCCCTATGGTCTTCCTTATGGCCTACATGGTTGCTGGATTCCCAAGAATTAG

Coding sequence (CDS)

ATGGCTTCCATGGCTGCCGGCAGCTGCATGTCTTCCTTCAACTTCCAATCCATCCACCCTCCAAAACGCACCGTTTCCAACTTCACCATTAGAAATATAGCCACCCCCATCCGTCCTCCATCTGTCGTTGTTCCATTGCCCGAGATCGACACTCCTGCCCACCCGAATCACGTTGCATGGACTAGCGTGCAACAAGAGCAATGGGAAGGAGAACTCTCCGTTCAAGGCCAATTGCCCCGTTGGCTGAATGGGACGTACCTGAGAAATGGACCTGGAATATGGCATATTGGCGATTATAATTTTCGGCATCTCTTCGATGGCTATGCTACAATTGTTAAGCTACAGTTTGATGATGGTCGACTAATTGCAGGCCATCGCCAGATCGAATCAGATGCGTATAAAGCTGCTATGAAGAATCAAAAGATTTGTTATCGTGAATTCTCTGAAGTACCCAAGACTAATAACTTTCTTGCCTACGTTGGGGAGCTTGCAAGTCTTTTCTCTGGTGCATCATTGACTGATAATGCAAACACTGGGGTTGTGAAACTTGGCGATGGCCGAGTCATTTGCCTTACAGAAACTCAAAAAGGGTCAATAATGATCAACCCAACCACGTTAGAGACTGTGGGGAAGTTTGAGTATAGCGATTCACTTGGCGGTTTAATTCACTCAGCTCATCCAATTGTTACAGATTTTGAATTTCTTACATTGTTGCCAGATCTTTTGAACCCAGGTTATTTGGTGGTTCGTATGGAACCGGGCTCAAATGAGCGTAAGGTTATCAGTCGGGTTAACTGCAAGGGCGGACCAGCCCCAGGTTGGGTCCACTCGTTTCCGGTGACGGAGAATTACGTGGTGGTGCCGGAGATGCCATTGAGATATTGCGCTCAAAACTTGTTGAGGGCGGAGCCAACGCCGTTGTACAAGTTTGAGTGGCGGCCTGAGTCCAAAGCCTATGTGCATGTCATGTGCAAAGCTAGTGGAAAAATTGTAGCATCTGTTGAAGTACCATTATACATTACGTTTCATTTCATAAATGCATACGAGGAGAGGGACGAAAAGGGAAGGATTACAGCCGTGATAGCCGATTGTTGCGAACATAACGCTAACCCGCTAATCTTGGACCGACTTCGGCTTCATAATTTGCGTCAATTCACTCCCAACGATGCCCTTCCAGATGCTAGGCTAGGAAGGTTCAGAATACCACTGGATGGGAGTGGATATGGGGAGCTGGAGGCAGCATTGGACCCAGACGAACATGCTTCAACCCTGCTTACTTGGGCAAGAAATATAGATATGCTTATGCCTATTGATTTGGTGAAGAAGAAGGCAAAGAATTGGCACGAGGCAGGTGCTATACCTTCCGAGCCCTTCTTCGTGGCTCGACCTGGTGCAACAAAAGAAGATGATGGGGTGGTGATCTCTATGGTCAGTGGGGAAGATGGAGAAGGGTATGCTTTGCTACTAGATGGATCCACATTTGAAGAGATAGCCAGAGCAAAATTTCCCTATGGTCTTCCTTATGGCCTACATGGTTGCTGGATTCCCAAGAATTAG

Protein sequence

MASMAAGSCMSSFNFQSIHPPKRTVSNFTIRNIATPIRPPSVVVPLPEIDTPAHPNHVAWTSVQQEQWEGELSVQGQLPRWLNGTYLRNGPGIWHIGDYNFRHLFDGYATIVKLQFDDGRLIAGHRQIESDAYKAAMKNQKICYREFSEVPKTNNFLAYVGELASLFSGASLTDNANTGVVKLGDGRVICLTETQKGSIMINPTTLETVGKFEYSDSLGGLIHSAHPIVTDFEFLTLLPDLLNPGYLVVRMEPGSNERKVISRVNCKGGPAPGWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAYVHVMCKASGKIVASVEVPLYITFHFINAYEERDEKGRITAVIADCCEHNANPLILDRLRLHNLRQFTPNDALPDARLGRFRIPLDGSGYGELEAALDPDEHASTLLTWARNIDMLMPIDLVKKKAKNWHEAGAIPSEPFFVARPGATKEDDGVVISMVSGEDGEGYALLLDGSTFEEIARAKFPYGLPYGLHGCWIPKN
BLAST of CmaCh12G002520.1 vs. Swiss-Prot
Match: CCD8B_ORYSJ (Carotenoid cleavage dioxygenase 8 homolog B, chloroplastic OS=Oryza sativa subsp. japonica GN=CCD8B PE=1 SV=1)

HSP 1 Score: 738.4 bits (1905), Expect = 5.3e-212
Identity = 349/533 (65.48%), Postives = 415/533 (77.86%), Query Frame = 1

Query: 19  HPPKRTVSNFTIRNIATPIRPPSVVV--PLPEIDTPAHPNH----------VAWTSVQQE 78
           H  KR V+     +  T   PP+ VV  P   +D P               VAW SV+QE
Sbjct: 32  HQGKRAVAQPLAASAVTEAAPPAPVVAPPARPVDAPRRRGGRGGGGGGGELVAWKSVRQE 91

Query: 79  QWEGELSVQGQLPRWLNGTYLRNGPGIWHIGDYNFRHLFDGYATIVKLQFDDGRLIAGHR 138
           +WEG L V G+LP WL+GTYLRNGPG+W++GDY FRHLFDGYAT+V++ F  GR +  HR
Sbjct: 92  RWEGALEVDGELPLWLDGTYLRNGPGLWNLGDYGFRHLFDGYATLVRVSFRGGRAVGAHR 151

Query: 139 QIESDAYKAAMKNQKICYREFSEVPKTNNFLAYVGELASLFSGASLTDNANTGVVKLGDG 198
           QIES+AYKAA  + K+CYREFSEVPK +NFL+YVG+LA+LFSG+SLTDN+NTGVV LGDG
Sbjct: 152 QIESEAYKAARAHGKVCYREFSEVPKPDNFLSYVGQLATLFSGSSLTDNSNTGVVMLGDG 211

Query: 199 RVICLTETQKGSIMINPTTLETVGKFEYSDSLGGLIHSAHPIVTDFEFLTLLPDLLNPGY 258
           RV+CLTET KGSI ++P TL+TVGKF+Y+D LGGLIHSAHPIVTD EF TL+PDL+ PGY
Sbjct: 212 RVLCLTETIKGSIQVDPDTLDTVGKFQYTDKLGGLIHSAHPIVTDTEFWTLIPDLIRPGY 271

Query: 259 LVVRMEPGSNERKVISRVNCKGGPAPGWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTP 318
           +V RM+ GSNER+ + RV+C+GGPAPGWVHSFPVTE+YVVVPEMPLRYCA+NLLRAEPTP
Sbjct: 272 VVARMDAGSNERQFVGRVDCRGGPAPGWVHSFPVTEHYVVVPEMPLRYCAKNLLRAEPTP 331

Query: 319 LYKFEWRPESKAYVHVMCKASGKIVASVEVPLYITFHFINAYEERDEKGRITAVIADCCE 378
           LYKFEW  ES +Y+HVMCKASGKIVASVEVP ++TFHFINAYEE DE+GR+TA+IADCCE
Sbjct: 332 LYKFEWHLESGSYMHVMCKASGKIVASVEVPPFVTFHFINAYEETDEEGRVTAIIADCCE 391

Query: 379 HNANPLILDRLRLHNLRQFTPNDALPDARLGRFRIPLDGSGYGELEAALDPDEHASTLLT 438
           HNAN  ILD+LRLHNLR  +  D LPDAR+GRFRIPLDGS +GELE ALDP+EH   +  
Sbjct: 392 HNANTAILDKLRLHNLRSSSGQDVLPDARVGRFRIPLDGSQFGELETALDPEEHGRGMDM 451

Query: 439 WARN----------------------IDMLMPIDLVKKKAKNWHEAGAIPSEPFFVARPG 498
            + N                       + L  +DLV++ AKNWHE G++PSEPFFV RPG
Sbjct: 452 CSINPAHVGREYRYAYACGARRPCNFPNTLTKVDLVERTAKNWHEEGSVPSEPFFVPRPG 511

Query: 499 ATKEDDGVVISMVSGEDGEGYALLLDGSTFEEIARAKFPYGLPYGLHGCWIPK 518
           AT+EDDGV ISMVS +DG GYAL+LDG TFEE+ARAKFPYGLPYGLH CW+P+
Sbjct: 512 ATEEDDGVAISMVSAKDGSGYALVLDGKTFEEVARAKFPYGLPYGLHCCWVPR 564

BLAST of CmaCh12G002520.1 vs. Swiss-Prot
Match: CCD8_ARATH (Carotenoid cleavage dioxygenase 8, chloroplastic OS=Arabidopsis thaliana GN=CCD8 PE=1 SV=1)

HSP 1 Score: 668.7 bits (1724), Expect = 5.2e-191
Identity = 322/505 (63.76%), Postives = 388/505 (76.83%), Query Frame = 1

Query: 45  PLPEIDTPAHPN--HVAWTSVQQEQWEGELSVQGQLPRWLNGTYLRNGPGIWHIGDYNFR 104
           PLPE +        HVAWTSVQQE WEGEL+VQG++P WLNGTYLRNGPG+W+IGD++FR
Sbjct: 66  PLPEKEKVEGERRCHVAWTSVQQENWEGELTVQGKIPTWLNGTYLRNGPGLWNIGDHDFR 125

Query: 105 HLFDGYATIVKLQFDDGRLIAGHRQIESDAYKAAMKNQKICYREFSEVPKT----NNFLA 164
           HLFDGY+T+VKLQFD GR+ A HR +ESDAYKAA K+ ++CYREFSE PK+     N  +
Sbjct: 126 HLFDGYSTLVKLQFDGGRIFAAHRLLESDAYKAAKKHNRLCYREFSETPKSVIINKNPFS 185

Query: 165 YVGELASLFSGASLTDNANTGVVKLGDGRVICLTETQKGSIMINPTTLETVGKFEYSDSL 224
            +GE+  LFSG SLTDNANTGV+KLGDGRV+CLTETQKGSI+++  TLET+GKFEY D L
Sbjct: 186 GIGEIVRLFSGESLTDNANTGVIKLGDGRVMCLTETQKGSILVDHETLETIGKFEYDDVL 245

Query: 225 GG-LIHSAHPIVTDFEFLTLLPDLLNPGYLVVRMEPGSNERKVISRVNCKGGP-APGWVH 284
              +I SAHPIVT+ E  TL+PDL+ PGY VVRME GSN+R+V+ RV C+ G   PGWVH
Sbjct: 246 SDHMIQSAHPIVTETEMWTLIPDLVKPGYRVVRMEAGSNKREVVGRVRCRSGSWGPGWVH 305

Query: 285 SFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAYVHVMCKASGKIVASVEV 344
           SF VTENYVV+PEMPLRY  +NLLRAEPTPLYKFEW P+  A++HVM K +G++VASVEV
Sbjct: 306 SFAVTENYVVIPEMPLRYSVKNLLRAEPTPLYKFEWCPQDGAFIHVMSKLTGEVVASVEV 365

Query: 345 PLYITFHFINAYEE-RDEKGRITAVIADCCEHNANPLILDRLRLHNLRQFTPNDALPDAR 404
           P Y+TFHFINAYEE ++  G+ T +IADCCEHNA+  ILD LRL  LR    +D LPDAR
Sbjct: 366 PAYVTFHFINAYEEDKNGDGKATVIIADCCEHNADTRILDMLRLDTLRSSHGHDVLPDAR 425

Query: 405 LGRFRIPLDGSGYGELEAALDPDEHASTLLTWARN----------------------IDM 464
           +GRFRIPLDGS YG+LE A++ ++H   +   + N                       + 
Sbjct: 426 IGRFRIPLDGSKYGKLETAVEAEKHGRAMDMCSINPLYLGQKYRYVYACGAQRPCNFPNA 485

Query: 465 LMPIDLVKKKAKNWHEAGAIPSEPFFVARPGATKEDDGVVISMVSGEDGEGYALLLDGST 519
           L  +D+V+KK KNWHE G IPSEPFFV RPGAT EDDGVVIS+VS E+G  +A+LLDGS+
Sbjct: 486 LSKVDIVEKKVKNWHEHGMIPSEPFFVPRPGATHEDDGVVISIVSEENGGSFAILLDGSS 545

BLAST of CmaCh12G002520.1 vs. Swiss-Prot
Match: CCD8A_ORYSJ (Carotenoid cleavage dioxygenase 8 homolog A, chloroplastic OS=Oryza sativa subsp. japonica GN=CCD8A PE=2 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 1.0e-170
Identity = 288/484 (59.50%), Postives = 357/484 (73.76%), Query Frame = 1

Query: 59  AWTSVQQEQWEGELSVQGQLPRWLNGTYLRNGPGIWHIGDYNFRHLFDGYATIVKLQFDD 118
           A TSV+QE+WEG+L ++G LP WLNGTY+RNGPG+W +G++ F HLFDGYAT+V++ F  
Sbjct: 68  ASTSVRQERWEGDLPIEGCLPPWLNGTYIRNGPGMWDVGEHAFHHLFDGYATLVRVSFRG 127

Query: 119 G---RLIAGHRQIESDAYKAAMKNQKICYREFSEVPK-TNNFLAYVGELASLFSGASLTD 178
           G   R    HRQIES+AY+AA+   +   REFS  P    + L   G+L  L +GA+LTD
Sbjct: 128 GGGARATGAHRQIESEAYRAAVARGRPVLREFSHCPAPAKSLLHRFGDLVGLVTGAALTD 187

Query: 179 NANTGVVKLGDGRVICLTETQKGSIMINPTTLETVGKFEYSDSLGGLIHSAHPIVTDFEF 238
           N N+ V+ LGDGRV+CLTET K S++I+P TLETVG+F Y+D LGG++ SAHPIVTD EF
Sbjct: 188 NPNSAVLPLGDGRVMCLTETTKSSVLIDPDTLETVGRFRYTDRLGGMVQSAHPIVTDTEF 247

Query: 239 LTLLPDLLNPGYLVVRMEPGSNERKVISRVNCKGGPAPGWVHSFPVTENYVVVPEMPLRY 298
           LTLLPDL+ PG+LVVRME GSNERKVI R++C+GGP+PGW+HSF VTE Y VVPEMPLRY
Sbjct: 248 LTLLPDLVRPGHLVVRMEAGSNERKVIGRMDCRGGPSPGWLHSFAVTEKYAVVPEMPLRY 307

Query: 299 CAQNLLRAEPTPLYKFEWRPESKAYVHVMCKASGKIVASVEVPLYITFHFINAYEERDEK 358
            + +LL +E  P Y F+W P S +Y+HVMCK++GK VASVEVP ++  HFINAYE   E+
Sbjct: 308 SSASLLASELAPFYAFDWVPASGSYMHVMCKSTGKTVASVEVPPFMAIHFINAYE---EE 367

Query: 359 GRITAVIADCCEHNANPLILDRLRLHNLRQFTPNDALPDARLGRFRIPLDGSGYGELEAA 418
           G   AV+ DCCEH  +P I++ L L  LR     D LP+AR+GRFRIPLDGS +GELE A
Sbjct: 368 GDEAAVVVDCCEHYGDPAIIETLVLSRLRLLRGKDVLPNARVGRFRIPLDGSPFGELETA 427

Query: 419 LDPDEHASTLLTWARN----------------------IDMLMPIDLVKKKAKNWHEAGA 478
           LDP+EH   +   + N                       + L  IDLV+KKAK+WHE G+
Sbjct: 428 LDPEEHGRGMDMCSINPARLGRKYQYAYACGARRPCNFPNTLTKIDLVEKKAKSWHEEGS 487

Query: 479 IPSEPFFVARPGATKEDDGVVISMVSGEDGEGYALLLDGSTFEEIARAKFPYGLPYGLHG 517
           +PSEPFFVARPGAT EDDGVVIS+VS +DGEGYAL+LD +TFEEIAR +FPYGLPYG HG
Sbjct: 488 VPSEPFFVARPGATDEDDGVVISIVSSDDGEGYALVLDATTFEEIARVRFPYGLPYGFHG 547

BLAST of CmaCh12G002520.1 vs. Swiss-Prot
Match: NINAB_GALME (Carotenoid isomerooxygenase OS=Galleria mellonella GN=ninaB PE=1 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 8.9e-42
Identity = 133/491 (27.09%), Postives = 222/491 (45.21%), Query Frame = 1

Query: 71  ELSVQGQLPRWLNGTYLRNGPGIWHIGDYNFRHLFDGYATIVKLQFDDGRLIAGHRQIES 130
           E ++ G++P WL G+ LRNGPG   +G   F HLFD  A + +   +DG +    R ++S
Sbjct: 30  EGTITGEIPSWLQGSLLRNGPGSLKVGSMRFEHLFDSSALLHRFAINDGSVTYQCRFLQS 89

Query: 131 DAYKAAMKNQKICYREFSEVPKTNNFLAYVGELASLFS-GASLTDNANTGVVKLGDGRVI 190
           + +K     ++I   EF      +        +A+LF  G SL+DNA   +   GD  + 
Sbjct: 90  NTFKKNRAAERIVVTEFGTRAVPDPCHTIFDRVAALFKPGESLSDNAMISLYPFGD-EIY 149

Query: 191 CLTETQKGSIM--INPTTLETVGKFEYSDSLGGLIHSAHPIVTDFEFLTLLPDLLNPGYL 250
             TE   G ++  I+P TL+T+ +    DS+  + H++HP V     +  L   +  G L
Sbjct: 150 AFTE---GPVIHRIDPETLDTLERRNLMDSVSLVNHTSHPHVMPNGDVYNLGMSIVQGRL 209

Query: 251 --VVRMEPGSNERKVISRVNCKGGPAPGW------VHSFPVTENYVVVPEMPLRYCAQNL 310
             V+   P + +  + ++ +     +P W      +H+F +TENY V+ E PL      +
Sbjct: 210 KHVIVKFPYTEKGDMFAKAHIVANMSPRWPLHPAYMHTFGITENYFVIVEQPLSVSLLTM 269

Query: 311 LRAEPT--PL-YKFEWRPESKAYVHVMCKASGKIVASVEVPLYITFHFINAYEERDEKGR 370
           ++++P+  PL     W P  + ++ ++ +  GK V           H INAYE       
Sbjct: 270 VKSQPSNEPLASSLHWYPNHETHIVLLSRRDGKEVKRYRTEPLFYLHIINAYEHDG---- 329

Query: 371 ITAVIADCCEHNANPLILDRLRLHNLRQFTPNDALPD---ARLGRFRIPLDGSGYGELEA 430
              ++ D C +  +   +D + ++ +     N    +    R  R  +PL+ +    +E 
Sbjct: 330 --VLVVDLCAYK-DAKAIDAMYINAIETMQSNADYAEWFRGRPKRLELPLNATNCSRIEP 389

Query: 431 AL-------DPDEH-------------ASTLLTWARNIDMLMPIDLVKKKAKNWHEAGAI 490
            L        P  H             A +    A N   ++ +D    + K W +    
Sbjct: 390 RLLAQLGCETPRIHYDLYNGRPYRYFYAISSDVDAANPGTIIKVDTKTGETKTWCDTNCY 449

Query: 491 PSEPFFVARPGATKEDDGVVISMV----SGEDGEGYALL-LDGSTFEEIARAKF--PYGL 518
           PSEP FV  PGAT+EDDGV++S +    +G      ALL L+     E+ARA F  P  +
Sbjct: 450 PSEPIFVPAPGATEEDDGVILSALVWGGAGAHCRRVALLVLEARGLRELARATFCAPSPV 509

BLAST of CmaCh12G002520.1 vs. Swiss-Prot
Match: RPE65_AMBTI (Retinoid isomerohydrolase OS=Ambystoma tigrinum GN=RPE65 PE=2 SV=3)

HSP 1 Score: 171.0 bits (432), Expect = 3.4e-41
Identity = 149/535 (27.85%), Postives = 220/535 (41.12%), Query Frame = 1

Query: 49  IDTPAHPNHVAWTSVQQEQWEGELSVQGQLPRWLNGTYLRNGPGIWHIGDYNFRHLFDGY 108
           +D PA      + S ++        V G++P WL+G+ LR GPG++ +G   F HLFDG 
Sbjct: 5   VDHPAGGYKKLFESTEELVAPVTAQVTGRIPVWLSGSLLRCGPGLFEVGSEQFYHLFDGQ 64

Query: 109 ATIVKLQFDDGRLIAGHRQIESDAYKAAMKNQKICYREFSEVPKTNNFLAYVGELASLFS 168
           A + K +F  G +I   R I +D Y  AM  ++I   EF      +          S F 
Sbjct: 65  ALLHKFEFKGGHVIYHRRFIRTDTYVRAMTEKRIVITEFGTFAFPDPCKNIFSRFLSYFQ 124

Query: 169 GASLTDNANTGVVKLGDGRVICLTETQKGSIMINPTTLETVGKFEYSD--SLGGLIHSAH 228
           G  +TDNA   V  +G+    C TET      INP TLETV K +  +  S+ G+  +AH
Sbjct: 125 GLEVTDNALVNVYPVGEDYYAC-TETNY-ITKINPETLETVKKVDLCNYVSINGV--TAH 184

Query: 229 PIV----TDFEFLTLLPDLLNPGYLVVRMEP-------GSNERKVISRVNCKGGPAPGWV 288
           P +    T +             Y +V++ P         N+ KV+ +  C     P +V
Sbjct: 185 PHIEHDGTVYNIGNCFGKHFAFAYNIVKIPPLQADKEDPINKAKVVVQFPCSERFKPSYV 244

Query: 289 HSFPVTENYVVVPEMPLRYCAQNLLRA----EPTPLYKFEWRPESKAYVHVMCKASGKIV 348
           HSF +T NY+V  E P++      L +        +  FE       ++HV  K +G+ +
Sbjct: 245 HSFGLTPNYIVFVEQPVKINLFKFLSSWSIWGANYMDCFESHETMGVWMHVAEKHTGEYL 304

Query: 349 -ASVEVPLYITFHFINAYEERDEKGRITAVIADCCEHNANPLILDRLRLHNLRQFTPN-- 408
                   +  FH IN YE+         +I D C       + + L L NLR+      
Sbjct: 305 NIKYRTSAFNLFHHINTYEDHG------FLIVDLCCWKGFEFVYNYLYLANLRENWEEVK 364

Query: 409 ---DALPDARLGRFRIPLD-----------GSGYGELEAALDPDE----HASTLLTWARN 468
              +  P   + R+ +PLD              Y    A L  DE        L +  R 
Sbjct: 365 RSAEKPPQPEVRRYVLPLDIHKVDTGKNLVNLPYTTATAVLRSDETIWLEPEVLFSGPRQ 424

Query: 469 I-----------------------------DMLMPIDLVKKKAKNWHEAGAIPSEPFFVA 513
                                         D L  +++  K+   W E    PSEP FV+
Sbjct: 425 AFEFPQINYKKHGGKDYTYAYGVGLNHFVPDRLSKLNVKTKETWVWQEPDTYPSEPIFVS 484

BLAST of CmaCh12G002520.1 vs. TrEMBL
Match: A0A023VSV7_CUCME (Carotenoid cleavage dioxygenase 8 OS=Cucumis melo GN=CCD8 PE=2 SV=1)

HSP 1 Score: 896.3 bits (2315), Expect = 1.7e-257
Identity = 444/551 (80.58%), Postives = 480/551 (87.11%), Query Frame = 1

Query: 1   MASMAAGSCM-SSFNFQSIHP--------PKRTVSNFTIRNIATPIRPPS-VVVPLPEID 60
           MASMA GSC+ S FNF++ HP         K T+SNFTIR+IA+P+RPPS VVVPLPEID
Sbjct: 2   MASMAIGSCIISPFNFKTKHPLLPIQQPSSKPTLSNFTIRSIASPVRPPSPVVVPLPEID 61

Query: 61  TPAHPNHVAWTSVQQEQWEGELSVQGQLPRWL-NGTYLRNGPGIWHIGDYNFRHLFDGYA 120
           T   PNHVAWTS++Q++WEGELSVQG LP WL +GTYLRNGPG+W+IGDYNFRHLFDGYA
Sbjct: 62  TFDAPNHVAWTSIRQDRWEGELSVQGHLPSWLVSGTYLRNGPGLWNIGDYNFRHLFDGYA 121

Query: 121 TIVKLQFDDGRLIAGHRQIESDAYKAAMKNQKICYREFSEVPKTNNFLAYVGELASLFSG 180
            IVKL FD+GRLIAGHRQIES+AYKAAMKNQKICYREFSEVPK +NFLAYVGELA+LFSG
Sbjct: 122 MIVKLHFDNGRLIAGHRQIESNAYKAAMKNQKICYREFSEVPKADNFLAYVGELANLFSG 181

Query: 181 ASLTDNANTGVVKLGDGRVICLTETQKGSIMINPTTLETVGKFEYSDSLGGLIHSAHPIV 240
           ASLTDNANTGVVKLGDGRV+CLTETQKGSIMI+P TLETVGKFEYSDSLGGLIHSAHPIV
Sbjct: 182 ASLTDNANTGVVKLGDGRVVCLTETQKGSIMIDPDTLETVGKFEYSDSLGGLIHSAHPIV 241

Query: 241 TDFEFLTLLPDLLNPGYLVVRMEPGSNERKVISRVNCKGGPAPGWVHSFPVTENYVVVPE 300
           TD EFLTLLPDLLNPGYLVVRMEP SNERKVI RVNCKGGPAPGWVHSFPVTENYVVVPE
Sbjct: 242 TDSEFLTLLPDLLNPGYLVVRMEPSSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPE 301

Query: 301 MPLRYCAQNLLRAEPTPLYKFEWRPESKAYVHVMCKASGKIVASVEVPLYITFHFINAYE 360
           MPLRYCAQNLL+AEPTPLYKFEWRPESKA++HVMCKASG IVASVEVPL+ITFHFINAYE
Sbjct: 302 MPLRYCAQNLLKAEPTPLYKFEWRPESKAFMHVMCKASGNIVASVEVPLFITFHFINAYE 361

Query: 361 ERDEKGRITAVIADCCEHNANPLILDRLRLHNLRQFTPNDALPDARLGRFRIPLDGSGYG 420
           ERDEKGRITAVIADCCEHNANP+ILDRLRLHNLR  +    LPDAR+GRFRIPLDGSGYG
Sbjct: 362 ERDEKGRITAVIADCCEHNANPVILDRLRLHNLRD-SLKYPLPDARVGRFRIPLDGSGYG 421

Query: 421 ELEAALDPDEHASTLLTWARN----------------------IDMLMPIDLVKKKAKNW 480
           ELEAALDPDEH   +   + N                       + L  IDLVKK AKNW
Sbjct: 422 ELEAALDPDEHGRGMDMCSFNPAYLGKKYRYAYACGAERPCNFPNTLTKIDLVKKMAKNW 481

Query: 481 HEAGAIPSEPFFVARPGATKEDDGVVISMVSGEDGEGYALLLDGSTFEEIARAKFPYGLP 519
           +E G +PSEPFFVARPGAT+EDDGVVISMVSG++GEGYALLLDGSTFEEIARAKFPYGLP
Sbjct: 482 YEEGTVPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLP 541

BLAST of CmaCh12G002520.1 vs. TrEMBL
Match: A0A0A0LS17_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G373590 PE=4 SV=1)

HSP 1 Score: 889.4 bits (2297), Expect = 2.1e-255
Identity = 440/549 (80.15%), Postives = 477/549 (86.89%), Query Frame = 1

Query: 1   MASMAAGSCM-SSFNFQSIHP-------PKRTVSNFTIRNIATPIRPPS-VVVPLPEIDT 60
           MASMA GSC+ S FNF++ HP        K T+SNFTIR+IA+P+ PPS VVVPLPEIDT
Sbjct: 2   MASMAMGSCIISPFNFKTKHPILPIQPSSKPTLSNFTIRSIASPVHPPSPVVVPLPEIDT 61

Query: 61  PAHPNHVAWTSVQQEQWEGELSVQGQLPRWLNGTYLRNGPGIWHIGDYNFRHLFDGYATI 120
               NHVAWTS++Q++WEGELS+QG LP WL+GTYLRNGPG+W+IGDYNFRHLFDGYATI
Sbjct: 62  SN--NHVAWTSIRQDRWEGELSIQGHLPSWLSGTYLRNGPGLWNIGDYNFRHLFDGYATI 121

Query: 121 VKLQFDDGRLIAGHRQIESDAYKAAMKNQKICYREFSEVPKTNNFLAYVGELASLFSGAS 180
           VKL FD+GRLIAGHRQIES+AYKAAMKNQKICYREFSEVPK +NFLAYVGELA+LFSGAS
Sbjct: 122 VKLHFDNGRLIAGHRQIESNAYKAAMKNQKICYREFSEVPKADNFLAYVGELANLFSGAS 181

Query: 181 LTDNANTGVVKLGDGRVICLTETQKGSIMINPTTLETVGKFEYSDSLGGLIHSAHPIVTD 240
           LTDNANTGVVKLGDGRV+CLTETQKGSIMI+P TLETVG+FEYSDSLGGLIHSAHPIVTD
Sbjct: 182 LTDNANTGVVKLGDGRVVCLTETQKGSIMIDPDTLETVGQFEYSDSLGGLIHSAHPIVTD 241

Query: 241 FEFLTLLPDLLNPGYLVVRMEPGSNERKVISRVNCKGGPAPGWVHSFPVTENYVVVPEMP 300
            EFLTLLPDLLNPGYLVVRMEP SNERKVI RVNCKGGPAPGWVHSFPVTENYVVVPEMP
Sbjct: 242 SEFLTLLPDLLNPGYLVVRMEPSSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMP 301

Query: 301 LRYCAQNLLRAEPTPLYKFEWRPESKAYVHVMCKASGKIVASVEVPLYITFHFINAYEER 360
           LRYCAQNLLRAEPTPLYKFEWRPESKA++HVMCKASG IVASVEVPL+ITFHFINAYEER
Sbjct: 302 LRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGNIVASVEVPLFITFHFINAYEER 361

Query: 361 DEKGRITAVIADCCEHNANPLILDRLRLHNLRQFTPNDALPDARLGRFRIPLDGSGYGEL 420
           DEKGRITAVIADCCEHNA+P ILDRLRLHNLR  +    LPDAR+GRFRIPLDGSGYG+L
Sbjct: 362 DEKGRITAVIADCCEHNADPAILDRLRLHNLRD-SLKYPLPDARVGRFRIPLDGSGYGKL 421

Query: 421 EAALDPDEHASTLLTWARN----------------------IDMLMPIDLVKKKAKNWHE 480
           EAALDPDEH   +   + N                       + L  IDLVKK AKNW+E
Sbjct: 422 EAALDPDEHGRGMDMCSFNPAYLGKKYRYAYACGAERPCNFPNTLTKIDLVKKVAKNWYE 481

Query: 481 AGAIPSEPFFVARPGATKEDDGVVISMVSGEDGEGYALLLDGSTFEEIARAKFPYGLPYG 519
            G IPSEPFFVARPGAT+EDDGVVISMVS ++GEGYALLLDGSTFEEIARAKFPYGLPYG
Sbjct: 482 EGTIPSEPFFVARPGATEEDDGVVISMVSAQNGEGYALLLDGSTFEEIARAKFPYGLPYG 541

BLAST of CmaCh12G002520.1 vs. TrEMBL
Match: A0A059CT69_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_C02930 PE=4 SV=1)

HSP 1 Score: 814.3 bits (2102), Expect = 8.5e-233
Identity = 386/520 (74.23%), Postives = 442/520 (85.00%), Query Frame = 1

Query: 20  PPKRTVSNFTIRNIATPIRPPSVVVPLPEIDTPAHPNHVAWTSVQQEQWEGELSVQGQLP 79
           P + T++N T +  A+ I  PS+       +  + PNHVAW SV+Q +WEGEL V+G++P
Sbjct: 47  PQELTIANVTSQ--ASVISRPSLGK-----EVVSQPNHVAWMSVRQHRWEGELVVKGEIP 106

Query: 80  RWLNGTYLRNGPGIWHIGDYNFRHLFDGYATIVKLQFDDGRLIAGHRQIESDAYKAAMKN 139
            WLNG YLRNGPG+WH+GDYNFRHLFDGYAT+VKLQF++G+L+AGHRQIES+AYKAAMK+
Sbjct: 107 LWLNGIYLRNGPGLWHVGDYNFRHLFDGYATLVKLQFENGQLVAGHRQIESEAYKAAMKS 166

Query: 140 QKICYREFSEVPKTNNFLAYVGELASLFSGASLTDNANTGVVKLGDGRVICLTETQKGSI 199
            K+CYREFSEVPK +NFL+YVGELASLFSGASLTDNANTGV++LGDGRV+CLTETQKGSI
Sbjct: 167 NKVCYREFSEVPKADNFLSYVGELASLFSGASLTDNANTGVIRLGDGRVVCLTETQKGSI 226

Query: 200 MINPTTLETVGKFEYSDSLGGLIHSAHPIVTDFEFLTLLPDLLNPGYLVVRMEPGSNERK 259
           MI+PTTLET+GKFEY+DSLGGLIHSAHPIVTD EFLTLLPDL+NPGYLVVRMEPGSNERK
Sbjct: 227 MIDPTTLETLGKFEYTDSLGGLIHSAHPIVTDTEFLTLLPDLINPGYLVVRMEPGSNERK 286

Query: 260 VISRVNCKGGPAPGWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAY 319
           VI RVNC+GGPAPGWVHSFPVTE+YVVVPEMPLRYCAQNLLRAEPTPLYKFEW P+SK +
Sbjct: 287 VIGRVNCRGGPAPGWVHSFPVTEHYVVVPEMPLRYCAQNLLRAEPTPLYKFEWHPDSKGF 346

Query: 320 VHVMCKASGKIVASVEVPLYITFHFINAYEERDEKGRITAVIADCCEHNANPLILDRLRL 379
           +H+MCKASGKIVASVEVPLY+TFHFINAYEE DE GR+ AVIADCCEHNA+  IL++LRL
Sbjct: 347 MHIMCKASGKIVASVEVPLYVTFHFINAYEETDEDGRVVAVIADCCEHNADTTILEKLRL 406

Query: 380 HNLRQFTPNDALPDARLGRFRIPLDGSGYGELEAALDPDEHASTLLTWARN--------- 439
            NLR F   D LPDAR+GRF IPLDGS YG+LEAALDPDEH   +   + N         
Sbjct: 407 QNLRSFAGQDVLPDARVGRFIIPLDGSPYGKLEAALDPDEHGRGMDMCSINPAFLGKKYR 466

Query: 440 -------------IDMLMPIDLVKKKAKNWHEAGAIPSEPFFVARPGATKEDDGVVISMV 499
                         + L  IDLV+KKAKNWHE GA+PSEPFFVARPGAT+EDDGVVISM+
Sbjct: 467 FAYACGAQRPCNFPNTLTKIDLVEKKAKNWHEDGAVPSEPFFVARPGATEEDDGVVISMI 526

Query: 500 SGEDGEGYALLLDGSTFEEIARAKFPYGLPYGLHGCWIPK 518
           S ++G+GYALLLDGSTFEEIARAKFPYGLPYGLHGCW+PK
Sbjct: 527 SEKNGDGYALLLDGSTFEEIARAKFPYGLPYGLHGCWVPK 559

BLAST of CmaCh12G002520.1 vs. TrEMBL
Match: V4UI82_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10010609mg PE=4 SV=1)

HSP 1 Score: 811.6 bits (2095), Expect = 5.5e-232
Identity = 387/514 (75.29%), Postives = 439/514 (85.41%), Query Frame = 1

Query: 29  TIRNIATPIRPPSVVVPLP--EIDTPAHPNHVAWTSVQQEQWEGELSVQGQLPRWLNGTY 88
           T+ N+ATP RP  + VPLP  +       NHVAWTSV+QE+WEGEL VQG++P WL GTY
Sbjct: 44  TVTNVATPQRPV-IAVPLPPEKEKYSVERNHVAWTSVRQERWEGELVVQGEIPLWLKGTY 103

Query: 89  LRNGPGIWHIGDYNFRHLFDGYATIVKLQFDDGRLIAGHRQIESDAYKAAMKNQKICYRE 148
           LRNGPG+WHIGDYNFRHLFDGYAT+VKL  ++GRLIAGHRQIES+AYKAA +N+K+CYRE
Sbjct: 104 LRNGPGMWHIGDYNFRHLFDGYATLVKLHIENGRLIAGHRQIESEAYKAAKENKKLCYRE 163

Query: 149 FSEVPKTNNFLAYVGELASLFSGASLTDNANTGVVKLGDGRVICLTETQKGSIMINPTTL 208
           FSEVPK +NFL+YVGELASLFSGASLTDNANTGVVKLGDGRV+CLTETQKGSI+I+P TL
Sbjct: 164 FSEVPKPDNFLSYVGELASLFSGASLTDNANTGVVKLGDGRVVCLTETQKGSIIIDPDTL 223

Query: 209 ETVGKFEYSDSLGGLIHSAHPIVTDFEFLTLLPDLLNPGYLVVRMEPGSNERKVISRVNC 268
           +T+G+FEYSD+LGGLIHSAHPIVT+ EFLTLLPDL++PGY VVRMEPGSNERKVI RVNC
Sbjct: 224 DTIGRFEYSDTLGGLIHSAHPIVTESEFLTLLPDLVSPGYWVVRMEPGSNERKVIGRVNC 283

Query: 269 KGGPAPGWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAYVHVMCKA 328
           +GGPAPGWVHSFPVTE+YVVVPEM LRYCAQNLLRAEPTPLYKFEW P+SKA++HVMCKA
Sbjct: 284 RGGPAPGWVHSFPVTEHYVVVPEMSLRYCAQNLLRAEPTPLYKFEWHPDSKAFLHVMCKA 343

Query: 329 SGKIVASVEVPLYITFHFINAYEERDEKGRITAVIADCCEHNANPLILDRLRLHNLRQFT 388
           SGKIVASVEVPLYITFHFINAYEE+DE GR+ A+IADCCEHNA+  ILD+LRL+NLR F 
Sbjct: 344 SGKIVASVEVPLYITFHFINAYEEKDEDGRLIAIIADCCEHNADTTILDKLRLNNLRSFN 403

Query: 389 PNDALPDARLGRFRIPLDGSGYGELEAALDPDEHASTLLTWARN---------------- 448
             D LPDAR+GRFRIP DGS +GELEAALDPDEH   +   + N                
Sbjct: 404 GQDVLPDARVGRFRIPFDGSEFGELEAALDPDEHGRGMDMCSINPAYLGKMYRYAYACGA 463

Query: 449 ------IDMLMPIDLVKKKAKNWHEAGAIPSEPFFVARPGATKEDDGVVISMVSGEDGEG 508
                  + L  +DLV KKAKNW+E GA+PSEPFFVARPGAT+EDDGVVIS+VS  +GEG
Sbjct: 464 KRPCNFPNTLTKLDLVGKKAKNWYEEGAVPSEPFFVARPGATEEDDGVVISIVSERNGEG 523

Query: 509 YALLLDGSTFEEIARAKFPYGLPYGLHGCWIPKN 519
           +ALLLDGSTFEEIARAKFPYGLPYGLHGCW+PKN
Sbjct: 524 FALLLDGSTFEEIARAKFPYGLPYGLHGCWVPKN 556

BLAST of CmaCh12G002520.1 vs. TrEMBL
Match: V7AVA5_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_009G109700g PE=4 SV=1)

HSP 1 Score: 808.5 bits (2087), Expect = 4.6e-231
Identity = 383/529 (72.40%), Postives = 437/529 (82.61%), Query Frame = 1

Query: 18  IHPPKRTVSNFTIRNIATPIRPPSVVVPLPEIDTPA-------HPNHVAWTSVQQEQWEG 77
           I+  +R + + T+ N+ATP  P  +  P PE            H +HVAWTS+ QE+WEG
Sbjct: 38  INKGQRNLRDLTVANVATPSLPV-IAPPTPEEQRSGTTGGRRHHHHHVAWTSISQERWEG 97

Query: 78  ELSVQGQLPRWLNGTYLRNGPGIWHIGDYNFRHLFDGYATIVKLQFDDGRLIAGHRQIES 137
           EL V+GQ+P WL GTYLRNGPG+WHIGDYNFRHLFDGYAT+VKLQ  DGRL AGHRQIES
Sbjct: 98  ELQVEGQIPLWLKGTYLRNGPGMWHIGDYNFRHLFDGYATLVKLQLQDGRLFAGHRQIES 157

Query: 138 DAYKAAMKNQKICYREFSEVPKTNNFLAYVGELASLFSGASLTDNANTGVVKLGDGRVIC 197
           +AY+AA KNQKICYREFSEVPK  NFLAYVGELA+LFSGASLTDNANTGVVKLGDGRV+C
Sbjct: 158 EAYRAAKKNQKICYREFSEVPKAANFLAYVGELANLFSGASLTDNANTGVVKLGDGRVVC 217

Query: 198 LTETQKGSIMINPTTLETVGKFEYSDSLGGLIHSAHPIVTDFEFLTLLPDLLNPGYLVVR 257
           LTETQKGSI+I+P TLETVGKFEYSDSLGGLIHSAHP+VTD EF+TLLPDL+ PGYLVVR
Sbjct: 218 LTETQKGSIVIDPETLETVGKFEYSDSLGGLIHSAHPVVTDKEFVTLLPDLVRPGYLVVR 277

Query: 258 MEPGSNERKVISRVNCKGGPAPGWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKF 317
           MEPG++ER+VI RVNC+GGP+PGWVHSFPVT++YVVVPEMPLRYCAQNLL+AEPTPLYKF
Sbjct: 278 MEPGTSERRVIGRVNCRGGPSPGWVHSFPVTQHYVVVPEMPLRYCAQNLLKAEPTPLYKF 337

Query: 318 EWRPESKAYVHVMCKASGKIVASVEVPLYITFHFINAYEERDEKGRITAVIADCCEHNAN 377
           EW P+SKA++HVMCK SG IVASVEVPL++TFHFINAYEE DE GR+TA+IADCCEHN++
Sbjct: 338 EWHPQSKAFMHVMCKTSGNIVASVEVPLFVTFHFINAYEEEDEDGRVTAIIADCCEHNSD 397

Query: 378 PLILDRLRLHNLRQFTPNDALPDARLGRFRIPLDGSGYGELEAALDPDEHASTLLTWARN 437
           P ILDRLRL NLR F   D LPDAR+GRFRIPLDGS  G L+AALDPDEH   +   + N
Sbjct: 398 PTILDRLRLQNLRSFNGEDVLPDARVGRFRIPLDGSPSGTLDAALDPDEHGKGMDMCSIN 457

Query: 438 ----------------------IDMLMPIDLVKKKAKNWHEAGAIPSEPFFVARPGATKE 497
                                  + L  +DL  KKAKNWHE GA+PSEPFFVARPGAT+E
Sbjct: 458 PNHLGKKYRYAYACGARRPCNFPNTLTKLDLESKKAKNWHEEGAVPSEPFFVARPGATEE 517

Query: 498 DDGVVISMVSGEDGEGYALLLDGSTFEEIARAKFPYGLPYGLHGCWIPK 518
           DDGVVIS++S ++GEGYALLLDGSTFEEIARA+FPYGLPYGLHGCW+PK
Sbjct: 518 DDGVVISIISEKNGEGYALLLDGSTFEEIARARFPYGLPYGLHGCWVPK 565

BLAST of CmaCh12G002520.1 vs. TAIR10
Match: AT4G32810.1 (AT4G32810.1 carotenoid cleavage dioxygenase 8)

HSP 1 Score: 668.7 bits (1724), Expect = 2.9e-192
Identity = 322/505 (63.76%), Postives = 388/505 (76.83%), Query Frame = 1

Query: 45  PLPEIDTPAHPN--HVAWTSVQQEQWEGELSVQGQLPRWLNGTYLRNGPGIWHIGDYNFR 104
           PLPE +        HVAWTSVQQE WEGEL+VQG++P WLNGTYLRNGPG+W+IGD++FR
Sbjct: 66  PLPEKEKVEGERRCHVAWTSVQQENWEGELTVQGKIPTWLNGTYLRNGPGLWNIGDHDFR 125

Query: 105 HLFDGYATIVKLQFDDGRLIAGHRQIESDAYKAAMKNQKICYREFSEVPKT----NNFLA 164
           HLFDGY+T+VKLQFD GR+ A HR +ESDAYKAA K+ ++CYREFSE PK+     N  +
Sbjct: 126 HLFDGYSTLVKLQFDGGRIFAAHRLLESDAYKAAKKHNRLCYREFSETPKSVIINKNPFS 185

Query: 165 YVGELASLFSGASLTDNANTGVVKLGDGRVICLTETQKGSIMINPTTLETVGKFEYSDSL 224
            +GE+  LFSG SLTDNANTGV+KLGDGRV+CLTETQKGSI+++  TLET+GKFEY D L
Sbjct: 186 GIGEIVRLFSGESLTDNANTGVIKLGDGRVMCLTETQKGSILVDHETLETIGKFEYDDVL 245

Query: 225 GG-LIHSAHPIVTDFEFLTLLPDLLNPGYLVVRMEPGSNERKVISRVNCKGGP-APGWVH 284
              +I SAHPIVT+ E  TL+PDL+ PGY VVRME GSN+R+V+ RV C+ G   PGWVH
Sbjct: 246 SDHMIQSAHPIVTETEMWTLIPDLVKPGYRVVRMEAGSNKREVVGRVRCRSGSWGPGWVH 305

Query: 285 SFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAYVHVMCKASGKIVASVEV 344
           SF VTENYVV+PEMPLRY  +NLLRAEPTPLYKFEW P+  A++HVM K +G++VASVEV
Sbjct: 306 SFAVTENYVVIPEMPLRYSVKNLLRAEPTPLYKFEWCPQDGAFIHVMSKLTGEVVASVEV 365

Query: 345 PLYITFHFINAYEE-RDEKGRITAVIADCCEHNANPLILDRLRLHNLRQFTPNDALPDAR 404
           P Y+TFHFINAYEE ++  G+ T +IADCCEHNA+  ILD LRL  LR    +D LPDAR
Sbjct: 366 PAYVTFHFINAYEEDKNGDGKATVIIADCCEHNADTRILDMLRLDTLRSSHGHDVLPDAR 425

Query: 405 LGRFRIPLDGSGYGELEAALDPDEHASTLLTWARN----------------------IDM 464
           +GRFRIPLDGS YG+LE A++ ++H   +   + N                       + 
Sbjct: 426 IGRFRIPLDGSKYGKLETAVEAEKHGRAMDMCSINPLYLGQKYRYVYACGAQRPCNFPNA 485

Query: 465 LMPIDLVKKKAKNWHEAGAIPSEPFFVARPGATKEDDGVVISMVSGEDGEGYALLLDGST 519
           L  +D+V+KK KNWHE G IPSEPFFV RPGAT EDDGVVIS+VS E+G  +A+LLDGS+
Sbjct: 486 LSKVDIVEKKVKNWHEHGMIPSEPFFVPRPGATHEDDGVVISIVSEENGGSFAILLDGSS 545

BLAST of CmaCh12G002520.1 vs. TAIR10
Match: AT1G30100.1 (AT1G30100.1 nine-cis-epoxycarotenoid dioxygenase 5)

HSP 1 Score: 102.1 bits (253), Expect = 1.1e-21
Identity = 121/502 (24.10%), Postives = 199/502 (39.64%), Query Frame = 1

Query: 45  PLPEIDTPAHPNHVAWTSVQQEQWEGELSVQGQLPRWLNGTYLRNGPGIWHIGDYNFRHL 104
           PLP+   P H     +  V ++  +  LSV G++P  ++G YLRNG         +  HL
Sbjct: 102 PLPKTVDPRHQISGNYAPVPEQSVKSSLSVDGKIPDCIDGVYLRNGANPL-FEPVSGHHL 161

Query: 105 FDGYATIVKLQFDDGRLIAGHRQIESD-AYKAAMKNQKICYREFSEVPKTNN----FLAY 164
           FDG   +  ++  +G      R  E++   +       I  +   E+   +      L Y
Sbjct: 162 FDGDGMVHAVKITNGDASYSCRFTETERLVQEKQLGSPIFPKAIGELHGHSGIARLMLFY 221

Query: 165 VGELASLFSGASLTDNANTGVVKLGDGRVICLTETQKGSIMINPT-TLETVGKFEYSDSL 224
              L  L +  + T  AN G+V   D  +    +     + +     LET+G+F++   L
Sbjct: 222 ARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDLPYQVRVTDNGDLETIGRFDFDGQL 281

Query: 225 GGLIHSAHPIVTDF--EFLTLLPDLLNPGYLVVRMEPGSNERKVISRVNCKGGPAPGWVH 284
              +  AHP +     E   L  D++   YL         E+     +      +P  +H
Sbjct: 282 SSAM-IAHPKIDPVTKELFALSYDVVKKPYLKYFKFSPEGEKSPDVEIPL---ASPTMMH 341

Query: 285 SFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAYVHVMCKASGKIVASVEV 344
            F +TEN+VV+P+  + +   ++   + +P+ K++    S+  +          +  VE 
Sbjct: 342 DFAITENFVVIPDQQVVFKLSDMFLGK-SPV-KYDGEKISRFGILPRNAKDASEMVWVES 401

Query: 345 PLYITFHFINAYE--ERDEKGRITAVIADC----------CEHNANPLILDRLRLH---- 404
           P    FH  NA+E  E DE      VI  C          C+   N  +L  +RL+    
Sbjct: 402 PETFCFHLWNAWESPETDE----VVVIGSCMTPADSIFNECDEQLNS-VLSEIRLNLKTG 461

Query: 405 --NLRQFTPNDALPDARLGRFRIPLDG--SGYGELEAALDPDEHASTLLTWARNIDMLMP 464
               R   P     +   G     L G  + Y  L A  +P         W + +     
Sbjct: 462 KSTRRTIIPGSVQMNLEAGMVNRNLLGRKTRYAYL-AIAEP---------WPK-VSGFAK 521

Query: 465 IDLVKKKAKNWHEAG-AIPSEPFFVARP-GATKEDDGVVISMVSGEDG-EGYALLLDGST 516
           +DL   + KN    G     EPFF+ R   +  EDDG ++S V  E+  E    +++  T
Sbjct: 522 VDLSTGEVKNHFYGGKKYGGEPFFLPRGLESDGEDDGYIMSFVHDEESWESELHIVNAVT 580

BLAST of CmaCh12G002520.1 vs. TAIR10
Match: AT3G24220.1 (AT3G24220.1 nine-cis-epoxycarotenoid dioxygenase 6)

HSP 1 Score: 84.3 bits (207), Expect = 2.3e-16
Identity = 94/337 (27.89%), Postives = 146/337 (43.32%), Query Frame = 1

Query: 45  PLPEIDTPAHPNHVAWTSVQQEQWEGELSVQGQLPRWLNGTYLRNGPGIWHIGDYNFRHL 104
           PLP+   PA      +  V +   +  L V GQ+P  L G Y+RNG            HL
Sbjct: 90  PLPKPTDPAVQLSGNFAPVNECPVQNGLEVVGQIPSCLKGVYIRNGANPM-FPPLAGHHL 149

Query: 105 FDGYATI--VKLQFDDG-----RLIAGHRQIESDAYKAAMKNQKIC-YREFSEVPKTNNF 164
           FDG   I  V + FD+      R    +R ++  A   ++  + I      S + +   F
Sbjct: 150 FDGDGMIHAVSIGFDNQVSYSCRYTKTNRLVQETALGRSVFPKPIGELHGHSGLARLALF 209

Query: 165 LAYVGELASLFSGASLTDNANTGVVKLGDGRVICLTETQKG-SIMINPT-TLETVGKFEY 224
            A  G    L  G      AN GVV   +GR++ ++E      + I+    LET+G+F +
Sbjct: 210 TARAG--IGLVDGTRGMGVANAGVVFF-NGRLLAMSEDDLPYQVKIDGQGDLETIGRFGF 269

Query: 225 SDSLGGLIHSAHPIV--TDFEFLTLLPDLLNPGYL-VVRMEP-GSNERKVISRVNCKGGP 284
            D +   +  AHP V  T  +  TL  ++L   +L  ++    G   R V   +     P
Sbjct: 270 DDQIDSSV-IAHPKVDATTGDLHTLSYNVLKKPHLRYLKFNTCGKKTRDVEITL-----P 329

Query: 285 APGWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAYVHVMCK--ASG 344
            P  +H F +TEN+VV+P+  + +    ++R     +Y      E  A   V+ K   +G
Sbjct: 330 EPTMIHDFAITENFVVIPDQQMVFKLSEMIRGGSPVIYV----KEKMARFGVLSKQDLTG 389

Query: 345 KIVASVEVPLYITFHFINAYEERDEKGRITAVIADCC 366
             +  V+VP    FH  NA+EER E+G    V+   C
Sbjct: 390 SDINWVDVPDCFCFHLWNAWEERTEEGDPVIVVIGSC 412

BLAST of CmaCh12G002520.1 vs. TAIR10
Match: AT3G14440.1 (AT3G14440.1 nine-cis-epoxycarotenoid dioxygenase 3)

HSP 1 Score: 72.4 bits (176), Expect = 9.2e-13
Identity = 76/317 (23.97%), Postives = 134/317 (42.27%), Query Frame = 1

Query: 45  PLPEIDTPAHPNHVAWTSVQQEQWEGELSVQGQLPRWLNGTYLRNGPGIWHIGDYNFRHL 104
           PLP+   P+      +  V ++     L V G+LP  + G Y+RNG    H       H 
Sbjct: 112 PLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLH-EPVTGHHF 171

Query: 105 FDGYATIVKLQFDDGRLIAGHRQIESDAY-KAAMKNQKICYREFSEVPKTNNF----LAY 164
           FDG   +  ++F+ G      R  +++ + +     + +  +   E+          L Y
Sbjct: 172 FDGDGMVHAVKFEHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLFY 231

Query: 165 VGELASLFSGASLTDNANTGVVKLGDGRVICLTETQKG-SIMINPT-TLETVGKFEYSDS 224
               A +   A  T  AN G+V   +GR++ ++E      + I P   L+TVG+F++   
Sbjct: 232 ARAAAGIVDPAHGTGVANAGLVYF-NGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQ 291

Query: 225 LGGLIHSAHPIVT--DFEFLTLLPDLLNPGYL-VVRMEP-GSNERKVISRVNCKGGPAPG 284
           L   +  AHP V     E   L  D+++  YL   R  P G+    V  +++      P 
Sbjct: 292 LESTM-IAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLD-----QPT 351

Query: 285 WVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAYVHVMCKASGKIVAS 344
            +H F +TEN+VVVP+  + +    ++R     +Y  +    ++  +          +  
Sbjct: 352 MMHDFAITENFVVVPDQQVVFKLPEMIRGGSPVVY--DKNKVARFGILDKYAEDSSNIKW 411

Query: 345 VEVPLYITFHFINAYEE 351
           ++ P    FH  NA+EE
Sbjct: 412 IDAPDCFCFHLWNAWEE 418

BLAST of CmaCh12G002520.1 vs. TAIR10
Match: AT1G78390.1 (AT1G78390.1 nine-cis-epoxycarotenoid dioxygenase 9)

HSP 1 Score: 64.3 bits (155), Expect = 2.5e-10
Identity = 75/321 (23.36%), Postives = 133/321 (41.43%), Query Frame = 1

Query: 45  PLPEIDTPAHPNHVAWTSVQQEQWEGELSVQGQLPRWLNGTYLRNGPGIWHIGDYNFRHL 104
           P P+   PA      +  V ++     L V G +P  + G Y+RNG    H    +  HL
Sbjct: 172 PHPKTADPAVQIAGNFFPVPEKPVVHNLPVTGTVPECIQGVYVRNGANPLH-KPVSGHHL 231

Query: 105 FDGYATIVKLQFDDGRLIAGHRQIESDAYKAAMKNQKICYREFSEVPKTNNFL-AYVG-- 164
           FDG   +  ++FD+G +    R  E++     +  ++ C R     PK    L  ++G  
Sbjct: 232 FDGDGMVHAVRFDNGSVSYACRFTETNR----LVQERECGRPV--FPKAIGELHGHLGIA 291

Query: 165 -----ELASLFSGASLTDN---ANTGVVKLGDGRVICLTETQKG-SIMINPT-TLETVGK 224
                    LF     T     AN G+V   +G ++ ++E      + +  T  LET G+
Sbjct: 292 KLMLFNTRGLFGLVDPTGGLGVANAGLVYF-NGHLLAMSEDDLPYHVKVTQTGDLETSGR 351

Query: 225 FEYSDSLGGLIHSAHPIVTDF--EFLTLLPDLLNPGYLVVRMEPGSNERKVISRVNCKGG 284
           +++   L   +  AHP +     E   L  D+++  YL         E+     +     
Sbjct: 352 YDFDGQLKSTM-IAHPKIDPETRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEIPLD-- 411

Query: 285 PAPGWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAYVHVMCKASGK 344
             P  +H F +TEN+VV+P+  + +    ++R     +Y  + + +S+  +         
Sbjct: 412 -QPTMIHDFAITENFVVIPDQQVVFRLPEMIRGGSPVVY--DEKKKSRFGILNKNAKDAS 471

Query: 345 IVASVEVPLYITFHFINAYEE 351
            +  +EVP    FH  N++EE
Sbjct: 472 SIQWIEVPDCFCFHLWNSWEE 478

BLAST of CmaCh12G002520.1 vs. NCBI nr
Match: gi|659088500|ref|XP_008445014.1| (PREDICTED: carotenoid cleavage dioxygenase 8 homolog B, chloroplastic [Cucumis melo])

HSP 1 Score: 899.0 bits (2322), Expect = 3.7e-258
Identity = 443/550 (80.55%), Postives = 480/550 (87.27%), Query Frame = 1

Query: 1   MASMAAGSCM-SSFNFQSIHP--------PKRTVSNFTIRNIATPIRPPS-VVVPLPEID 60
           MASMA GSC+ S FNF++ HP         K T+SNFTIR+IA+P+RPPS VVVPLPEID
Sbjct: 2   MASMAIGSCIISPFNFKTKHPLLPIQQPSSKPTLSNFTIRSIASPVRPPSPVVVPLPEID 61

Query: 61  TPAHPNHVAWTSVQQEQWEGELSVQGQLPRWLNGTYLRNGPGIWHIGDYNFRHLFDGYAT 120
           T   PNHVAWTS++Q++WEGELSVQG LP WL+GTYLRNGPG+W+IGDY+FRHLFDGYA 
Sbjct: 62  TFDAPNHVAWTSIRQDRWEGELSVQGHLPSWLSGTYLRNGPGLWNIGDYDFRHLFDGYAM 121

Query: 121 IVKLQFDDGRLIAGHRQIESDAYKAAMKNQKICYREFSEVPKTNNFLAYVGELASLFSGA 180
           IVKL FD+GRLIAGHRQIES+AYKAAMKNQKICYREFSEVPK +NFLAYVGELA+LFSGA
Sbjct: 122 IVKLHFDNGRLIAGHRQIESNAYKAAMKNQKICYREFSEVPKADNFLAYVGELANLFSGA 181

Query: 181 SLTDNANTGVVKLGDGRVICLTETQKGSIMINPTTLETVGKFEYSDSLGGLIHSAHPIVT 240
           SLTDNANTGVVKLGDGRV+CLTETQKGSIMI+P TLETVGKFEYSDSLGGLIHSAHPIVT
Sbjct: 182 SLTDNANTGVVKLGDGRVVCLTETQKGSIMIDPDTLETVGKFEYSDSLGGLIHSAHPIVT 241

Query: 241 DFEFLTLLPDLLNPGYLVVRMEPGSNERKVISRVNCKGGPAPGWVHSFPVTENYVVVPEM 300
           D EFLTLLPDLLNPGYLVVRMEP SNERKVI RVNCKGGPAPGWVHSFPVTENYVVVPEM
Sbjct: 242 DSEFLTLLPDLLNPGYLVVRMEPSSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEM 301

Query: 301 PLRYCAQNLLRAEPTPLYKFEWRPESKAYVHVMCKASGKIVASVEVPLYITFHFINAYEE 360
           PLRYCAQNLL+AEPTPLYKFEWRPESKA++HVMCKASG IVASVEVPL+ITFHFINAYEE
Sbjct: 302 PLRYCAQNLLKAEPTPLYKFEWRPESKAFMHVMCKASGNIVASVEVPLFITFHFINAYEE 361

Query: 361 RDEKGRITAVIADCCEHNANPLILDRLRLHNLRQFTPNDALPDARLGRFRIPLDGSGYGE 420
           RDEKGRITAVIADCCEHNANP+ILDRLRLHNLR  +    LPDAR+GRFRIPLDGSGYGE
Sbjct: 362 RDEKGRITAVIADCCEHNANPVILDRLRLHNLRD-SLKYPLPDARVGRFRIPLDGSGYGE 421

Query: 421 LEAALDPDEHASTLLTWARN----------------------IDMLMPIDLVKKKAKNWH 480
           LEAALDPDEH   +   + N                       + L  IDLVKK AKNW+
Sbjct: 422 LEAALDPDEHGRGMDMCSFNPAYLGKKYRYAYACGAERPCNFPNTLTKIDLVKKMAKNWY 481

Query: 481 EAGAIPSEPFFVARPGATKEDDGVVISMVSGEDGEGYALLLDGSTFEEIARAKFPYGLPY 519
           E G +PSEPFFVARPGAT+EDDGVVISMVSG++GEGYALLLDGSTFEEIARAKFPYGLPY
Sbjct: 482 EEGTVPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPY 541

BLAST of CmaCh12G002520.1 vs. NCBI nr
Match: gi|661902861|ref|NP_001284455.1| (carotenoid cleavage dioxygenase 8 homolog B, chloroplastic [Cucumis melo])

HSP 1 Score: 896.3 bits (2315), Expect = 2.4e-257
Identity = 444/551 (80.58%), Postives = 480/551 (87.11%), Query Frame = 1

Query: 1   MASMAAGSCM-SSFNFQSIHP--------PKRTVSNFTIRNIATPIRPPS-VVVPLPEID 60
           MASMA GSC+ S FNF++ HP         K T+SNFTIR+IA+P+RPPS VVVPLPEID
Sbjct: 2   MASMAIGSCIISPFNFKTKHPLLPIQQPSSKPTLSNFTIRSIASPVRPPSPVVVPLPEID 61

Query: 61  TPAHPNHVAWTSVQQEQWEGELSVQGQLPRWL-NGTYLRNGPGIWHIGDYNFRHLFDGYA 120
           T   PNHVAWTS++Q++WEGELSVQG LP WL +GTYLRNGPG+W+IGDYNFRHLFDGYA
Sbjct: 62  TFDAPNHVAWTSIRQDRWEGELSVQGHLPSWLVSGTYLRNGPGLWNIGDYNFRHLFDGYA 121

Query: 121 TIVKLQFDDGRLIAGHRQIESDAYKAAMKNQKICYREFSEVPKTNNFLAYVGELASLFSG 180
            IVKL FD+GRLIAGHRQIES+AYKAAMKNQKICYREFSEVPK +NFLAYVGELA+LFSG
Sbjct: 122 MIVKLHFDNGRLIAGHRQIESNAYKAAMKNQKICYREFSEVPKADNFLAYVGELANLFSG 181

Query: 181 ASLTDNANTGVVKLGDGRVICLTETQKGSIMINPTTLETVGKFEYSDSLGGLIHSAHPIV 240
           ASLTDNANTGVVKLGDGRV+CLTETQKGSIMI+P TLETVGKFEYSDSLGGLIHSAHPIV
Sbjct: 182 ASLTDNANTGVVKLGDGRVVCLTETQKGSIMIDPDTLETVGKFEYSDSLGGLIHSAHPIV 241

Query: 241 TDFEFLTLLPDLLNPGYLVVRMEPGSNERKVISRVNCKGGPAPGWVHSFPVTENYVVVPE 300
           TD EFLTLLPDLLNPGYLVVRMEP SNERKVI RVNCKGGPAPGWVHSFPVTENYVVVPE
Sbjct: 242 TDSEFLTLLPDLLNPGYLVVRMEPSSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPE 301

Query: 301 MPLRYCAQNLLRAEPTPLYKFEWRPESKAYVHVMCKASGKIVASVEVPLYITFHFINAYE 360
           MPLRYCAQNLL+AEPTPLYKFEWRPESKA++HVMCKASG IVASVEVPL+ITFHFINAYE
Sbjct: 302 MPLRYCAQNLLKAEPTPLYKFEWRPESKAFMHVMCKASGNIVASVEVPLFITFHFINAYE 361

Query: 361 ERDEKGRITAVIADCCEHNANPLILDRLRLHNLRQFTPNDALPDARLGRFRIPLDGSGYG 420
           ERDEKGRITAVIADCCEHNANP+ILDRLRLHNLR  +    LPDAR+GRFRIPLDGSGYG
Sbjct: 362 ERDEKGRITAVIADCCEHNANPVILDRLRLHNLRD-SLKYPLPDARVGRFRIPLDGSGYG 421

Query: 421 ELEAALDPDEHASTLLTWARN----------------------IDMLMPIDLVKKKAKNW 480
           ELEAALDPDEH   +   + N                       + L  IDLVKK AKNW
Sbjct: 422 ELEAALDPDEHGRGMDMCSFNPAYLGKKYRYAYACGAERPCNFPNTLTKIDLVKKMAKNW 481

Query: 481 HEAGAIPSEPFFVARPGATKEDDGVVISMVSGEDGEGYALLLDGSTFEEIARAKFPYGLP 519
           +E G +PSEPFFVARPGAT+EDDGVVISMVSG++GEGYALLLDGSTFEEIARAKFPYGLP
Sbjct: 482 YEEGTVPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLP 541

BLAST of CmaCh12G002520.1 vs. NCBI nr
Match: gi|449461315|ref|XP_004148387.1| (PREDICTED: carotenoid cleavage dioxygenase 8 homolog B, chloroplastic [Cucumis sativus])

HSP 1 Score: 889.4 bits (2297), Expect = 3.0e-255
Identity = 440/549 (80.15%), Postives = 477/549 (86.89%), Query Frame = 1

Query: 1   MASMAAGSCM-SSFNFQSIHP-------PKRTVSNFTIRNIATPIRPPS-VVVPLPEIDT 60
           MASMA GSC+ S FNF++ HP        K T+SNFTIR+IA+P+ PPS VVVPLPEIDT
Sbjct: 2   MASMAMGSCIISPFNFKTKHPILPIQPSSKPTLSNFTIRSIASPVHPPSPVVVPLPEIDT 61

Query: 61  PAHPNHVAWTSVQQEQWEGELSVQGQLPRWLNGTYLRNGPGIWHIGDYNFRHLFDGYATI 120
               NHVAWTS++Q++WEGELS+QG LP WL+GTYLRNGPG+W+IGDYNFRHLFDGYATI
Sbjct: 62  SN--NHVAWTSIRQDRWEGELSIQGHLPSWLSGTYLRNGPGLWNIGDYNFRHLFDGYATI 121

Query: 121 VKLQFDDGRLIAGHRQIESDAYKAAMKNQKICYREFSEVPKTNNFLAYVGELASLFSGAS 180
           VKL FD+GRLIAGHRQIES+AYKAAMKNQKICYREFSEVPK +NFLAYVGELA+LFSGAS
Sbjct: 122 VKLHFDNGRLIAGHRQIESNAYKAAMKNQKICYREFSEVPKADNFLAYVGELANLFSGAS 181

Query: 181 LTDNANTGVVKLGDGRVICLTETQKGSIMINPTTLETVGKFEYSDSLGGLIHSAHPIVTD 240
           LTDNANTGVVKLGDGRV+CLTETQKGSIMI+P TLETVG+FEYSDSLGGLIHSAHPIVTD
Sbjct: 182 LTDNANTGVVKLGDGRVVCLTETQKGSIMIDPDTLETVGQFEYSDSLGGLIHSAHPIVTD 241

Query: 241 FEFLTLLPDLLNPGYLVVRMEPGSNERKVISRVNCKGGPAPGWVHSFPVTENYVVVPEMP 300
            EFLTLLPDLLNPGYLVVRMEP SNERKVI RVNCKGGPAPGWVHSFPVTENYVVVPEMP
Sbjct: 242 SEFLTLLPDLLNPGYLVVRMEPSSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMP 301

Query: 301 LRYCAQNLLRAEPTPLYKFEWRPESKAYVHVMCKASGKIVASVEVPLYITFHFINAYEER 360
           LRYCAQNLLRAEPTPLYKFEWRPESKA++HVMCKASG IVASVEVPL+ITFHFINAYEER
Sbjct: 302 LRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGNIVASVEVPLFITFHFINAYEER 361

Query: 361 DEKGRITAVIADCCEHNANPLILDRLRLHNLRQFTPNDALPDARLGRFRIPLDGSGYGEL 420
           DEKGRITAVIADCCEHNA+P ILDRLRLHNLR  +    LPDAR+GRFRIPLDGSGYG+L
Sbjct: 362 DEKGRITAVIADCCEHNADPAILDRLRLHNLRD-SLKYPLPDARVGRFRIPLDGSGYGKL 421

Query: 421 EAALDPDEHASTLLTWARN----------------------IDMLMPIDLVKKKAKNWHE 480
           EAALDPDEH   +   + N                       + L  IDLVKK AKNW+E
Sbjct: 422 EAALDPDEHGRGMDMCSFNPAYLGKKYRYAYACGAERPCNFPNTLTKIDLVKKVAKNWYE 481

Query: 481 AGAIPSEPFFVARPGATKEDDGVVISMVSGEDGEGYALLLDGSTFEEIARAKFPYGLPYG 519
            G IPSEPFFVARPGAT+EDDGVVISMVS ++GEGYALLLDGSTFEEIARAKFPYGLPYG
Sbjct: 482 EGTIPSEPFFVARPGATEEDDGVVISMVSAQNGEGYALLLDGSTFEEIARAKFPYGLPYG 541

BLAST of CmaCh12G002520.1 vs. NCBI nr
Match: gi|702301872|ref|XP_010049109.1| (PREDICTED: carotenoid cleavage dioxygenase 8 homolog B, chloroplastic-like [Eucalyptus grandis])

HSP 1 Score: 814.3 bits (2102), Expect = 1.2e-232
Identity = 386/520 (74.23%), Postives = 442/520 (85.00%), Query Frame = 1

Query: 20  PPKRTVSNFTIRNIATPIRPPSVVVPLPEIDTPAHPNHVAWTSVQQEQWEGELSVQGQLP 79
           P + T++N T +  A+ I  PS+       +  + PNHVAW SV+Q +WEGEL V+G++P
Sbjct: 47  PQELTIANVTSQ--ASVISRPSLGK-----EVVSQPNHVAWMSVRQHRWEGELVVKGEIP 106

Query: 80  RWLNGTYLRNGPGIWHIGDYNFRHLFDGYATIVKLQFDDGRLIAGHRQIESDAYKAAMKN 139
            WLNG YLRNGPG+WH+GDYNFRHLFDGYAT+VKLQF++G+L+AGHRQIES+AYKAAMK+
Sbjct: 107 LWLNGIYLRNGPGLWHVGDYNFRHLFDGYATLVKLQFENGQLVAGHRQIESEAYKAAMKS 166

Query: 140 QKICYREFSEVPKTNNFLAYVGELASLFSGASLTDNANTGVVKLGDGRVICLTETQKGSI 199
            K+CYREFSEVPK +NFL+YVGELASLFSGASLTDNANTGV++LGDGRV+CLTETQKGSI
Sbjct: 167 NKVCYREFSEVPKADNFLSYVGELASLFSGASLTDNANTGVIRLGDGRVVCLTETQKGSI 226

Query: 200 MINPTTLETVGKFEYSDSLGGLIHSAHPIVTDFEFLTLLPDLLNPGYLVVRMEPGSNERK 259
           MI+PTTLET+GKFEY+DSLGGLIHSAHPIVTD EFLTLLPDL+NPGYLVVRMEPGSNERK
Sbjct: 227 MIDPTTLETLGKFEYTDSLGGLIHSAHPIVTDTEFLTLLPDLINPGYLVVRMEPGSNERK 286

Query: 260 VISRVNCKGGPAPGWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAY 319
           VI RVNC+GGPAPGWVHSFPVTE+YVVVPEMPLRYCAQNLLRAEPTPLYKFEW P+SK +
Sbjct: 287 VIGRVNCRGGPAPGWVHSFPVTEHYVVVPEMPLRYCAQNLLRAEPTPLYKFEWHPDSKGF 346

Query: 320 VHVMCKASGKIVASVEVPLYITFHFINAYEERDEKGRITAVIADCCEHNANPLILDRLRL 379
           +H+MCKASGKIVASVEVPLY+TFHFINAYEE DE GR+ AVIADCCEHNA+  IL++LRL
Sbjct: 347 MHIMCKASGKIVASVEVPLYVTFHFINAYEETDEDGRVVAVIADCCEHNADTTILEKLRL 406

Query: 380 HNLRQFTPNDALPDARLGRFRIPLDGSGYGELEAALDPDEHASTLLTWARN--------- 439
            NLR F   D LPDAR+GRF IPLDGS YG+LEAALDPDEH   +   + N         
Sbjct: 407 QNLRSFAGQDVLPDARVGRFIIPLDGSPYGKLEAALDPDEHGRGMDMCSINPAFLGKKYR 466

Query: 440 -------------IDMLMPIDLVKKKAKNWHEAGAIPSEPFFVARPGATKEDDGVVISMV 499
                         + L  IDLV+KKAKNWHE GA+PSEPFFVARPGAT+EDDGVVISM+
Sbjct: 467 FAYACGAQRPCNFPNTLTKIDLVEKKAKNWHEDGAVPSEPFFVARPGATEEDDGVVISMI 526

Query: 500 SGEDGEGYALLLDGSTFEEIARAKFPYGLPYGLHGCWIPK 518
           S ++G+GYALLLDGSTFEEIARAKFPYGLPYGLHGCW+PK
Sbjct: 527 SEKNGDGYALLLDGSTFEEIARAKFPYGLPYGLHGCWVPK 559

BLAST of CmaCh12G002520.1 vs. NCBI nr
Match: gi|567917368|ref|XP_006450690.1| (hypothetical protein CICLE_v10010609mg [Citrus clementina])

HSP 1 Score: 811.6 bits (2095), Expect = 7.9e-232
Identity = 387/514 (75.29%), Postives = 439/514 (85.41%), Query Frame = 1

Query: 29  TIRNIATPIRPPSVVVPLP--EIDTPAHPNHVAWTSVQQEQWEGELSVQGQLPRWLNGTY 88
           T+ N+ATP RP  + VPLP  +       NHVAWTSV+QE+WEGEL VQG++P WL GTY
Sbjct: 44  TVTNVATPQRPV-IAVPLPPEKEKYSVERNHVAWTSVRQERWEGELVVQGEIPLWLKGTY 103

Query: 89  LRNGPGIWHIGDYNFRHLFDGYATIVKLQFDDGRLIAGHRQIESDAYKAAMKNQKICYRE 148
           LRNGPG+WHIGDYNFRHLFDGYAT+VKL  ++GRLIAGHRQIES+AYKAA +N+K+CYRE
Sbjct: 104 LRNGPGMWHIGDYNFRHLFDGYATLVKLHIENGRLIAGHRQIESEAYKAAKENKKLCYRE 163

Query: 149 FSEVPKTNNFLAYVGELASLFSGASLTDNANTGVVKLGDGRVICLTETQKGSIMINPTTL 208
           FSEVPK +NFL+YVGELASLFSGASLTDNANTGVVKLGDGRV+CLTETQKGSI+I+P TL
Sbjct: 164 FSEVPKPDNFLSYVGELASLFSGASLTDNANTGVVKLGDGRVVCLTETQKGSIIIDPDTL 223

Query: 209 ETVGKFEYSDSLGGLIHSAHPIVTDFEFLTLLPDLLNPGYLVVRMEPGSNERKVISRVNC 268
           +T+G+FEYSD+LGGLIHSAHPIVT+ EFLTLLPDL++PGY VVRMEPGSNERKVI RVNC
Sbjct: 224 DTIGRFEYSDTLGGLIHSAHPIVTESEFLTLLPDLVSPGYWVVRMEPGSNERKVIGRVNC 283

Query: 269 KGGPAPGWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAYVHVMCKA 328
           +GGPAPGWVHSFPVTE+YVVVPEM LRYCAQNLLRAEPTPLYKFEW P+SKA++HVMCKA
Sbjct: 284 RGGPAPGWVHSFPVTEHYVVVPEMSLRYCAQNLLRAEPTPLYKFEWHPDSKAFLHVMCKA 343

Query: 329 SGKIVASVEVPLYITFHFINAYEERDEKGRITAVIADCCEHNANPLILDRLRLHNLRQFT 388
           SGKIVASVEVPLYITFHFINAYEE+DE GR+ A+IADCCEHNA+  ILD+LRL+NLR F 
Sbjct: 344 SGKIVASVEVPLYITFHFINAYEEKDEDGRLIAIIADCCEHNADTTILDKLRLNNLRSFN 403

Query: 389 PNDALPDARLGRFRIPLDGSGYGELEAALDPDEHASTLLTWARN---------------- 448
             D LPDAR+GRFRIP DGS +GELEAALDPDEH   +   + N                
Sbjct: 404 GQDVLPDARVGRFRIPFDGSEFGELEAALDPDEHGRGMDMCSINPAYLGKMYRYAYACGA 463

Query: 449 ------IDMLMPIDLVKKKAKNWHEAGAIPSEPFFVARPGATKEDDGVVISMVSGEDGEG 508
                  + L  +DLV KKAKNW+E GA+PSEPFFVARPGAT+EDDGVVIS+VS  +GEG
Sbjct: 464 KRPCNFPNTLTKLDLVGKKAKNWYEEGAVPSEPFFVARPGATEEDDGVVISIVSERNGEG 523

Query: 509 YALLLDGSTFEEIARAKFPYGLPYGLHGCWIPKN 519
           +ALLLDGSTFEEIARAKFPYGLPYGLHGCW+PKN
Sbjct: 524 FALLLDGSTFEEIARAKFPYGLPYGLHGCWVPKN 556

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CCD8B_ORYSJ5.3e-21265.48Carotenoid cleavage dioxygenase 8 homolog B, chloroplastic OS=Oryza sativa subsp... [more]
CCD8_ARATH5.2e-19163.76Carotenoid cleavage dioxygenase 8, chloroplastic OS=Arabidopsis thaliana GN=CCD8... [more]
CCD8A_ORYSJ1.0e-17059.50Carotenoid cleavage dioxygenase 8 homolog A, chloroplastic OS=Oryza sativa subsp... [more]
NINAB_GALME8.9e-4227.09Carotenoid isomerooxygenase OS=Galleria mellonella GN=ninaB PE=1 SV=1[more]
RPE65_AMBTI3.4e-4127.85Retinoid isomerohydrolase OS=Ambystoma tigrinum GN=RPE65 PE=2 SV=3[more]
Match NameE-valueIdentityDescription
A0A023VSV7_CUCME1.7e-25780.58Carotenoid cleavage dioxygenase 8 OS=Cucumis melo GN=CCD8 PE=2 SV=1[more]
A0A0A0LS17_CUCSA2.1e-25580.15Uncharacterized protein OS=Cucumis sativus GN=Csa_2G373590 PE=4 SV=1[more]
A0A059CT69_EUCGR8.5e-23374.23Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_C02930 PE=4 SV=1[more]
V4UI82_9ROSI5.5e-23275.29Uncharacterized protein OS=Citrus clementina GN=CICLE_v10010609mg PE=4 SV=1[more]
V7AVA5_PHAVU4.6e-23172.40Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_009G109700g PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G32810.12.9e-19263.76 carotenoid cleavage dioxygenase 8[more]
AT1G30100.11.1e-2124.10 nine-cis-epoxycarotenoid dioxygenase 5[more]
AT3G24220.12.3e-1627.89 nine-cis-epoxycarotenoid dioxygenase 6[more]
AT3G14440.19.2e-1323.97 nine-cis-epoxycarotenoid dioxygenase 3[more]
AT1G78390.12.5e-1023.36 nine-cis-epoxycarotenoid dioxygenase 9[more]
Match NameE-valueIdentityDescription
gi|659088500|ref|XP_008445014.1|3.7e-25880.55PREDICTED: carotenoid cleavage dioxygenase 8 homolog B, chloroplastic [Cucumis m... [more]
gi|661902861|ref|NP_001284455.1|2.4e-25780.58carotenoid cleavage dioxygenase 8 homolog B, chloroplastic [Cucumis melo][more]
gi|449461315|ref|XP_004148387.1|3.0e-25580.15PREDICTED: carotenoid cleavage dioxygenase 8 homolog B, chloroplastic [Cucumis s... [more]
gi|702301872|ref|XP_010049109.1|1.2e-23274.23PREDICTED: carotenoid cleavage dioxygenase 8 homolog B, chloroplastic-like [Euca... [more]
gi|567917368|ref|XP_006450690.1|7.9e-23275.29hypothetical protein CICLE_v10010609mg [Citrus clementina][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR004294Carotenoid_Oase
Vocabulary: Molecular Function
TermDefinition
GO:0016702oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0009926 auxin polar transport
biological_process GO:0016121 carotene catabolic process
biological_process GO:0009965 leaf morphogenesis
biological_process GO:0009733 response to auxin
biological_process GO:0010223 secondary shoot formation
biological_process GO:1901601 strigolactone biosynthetic process
biological_process GO:0016124 xanthophyll catabolic process
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmaCh12G002520CmaCh12G002520gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmaCh12G002520.1CmaCh12G002520.1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh12G002520.1.CDS.7CmaCh12G002520.1.CDS.7CDS
CmaCh12G002520.1.CDS.6CmaCh12G002520.1.CDS.6CDS
CmaCh12G002520.1.CDS.5CmaCh12G002520.1.CDS.5CDS
CmaCh12G002520.1.CDS.4CmaCh12G002520.1.CDS.4CDS
CmaCh12G002520.1.CDS.3CmaCh12G002520.1.CDS.3CDS
CmaCh12G002520.1.CDS.2CmaCh12G002520.1.CDS.2CDS
CmaCh12G002520.1.CDS.1CmaCh12G002520.1.CDS.1CDS


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh12G002520.1.exon.7CmaCh12G002520.1.exon.7exon
CmaCh12G002520.1.exon.6CmaCh12G002520.1.exon.6exon
CmaCh12G002520.1.exon.5CmaCh12G002520.1.exon.5exon
CmaCh12G002520.1.exon.4CmaCh12G002520.1.exon.4exon
CmaCh12G002520.1.exon.3CmaCh12G002520.1.exon.3exon
CmaCh12G002520.1.exon.2CmaCh12G002520.1.exon.2exon
CmaCh12G002520.1.exon.1CmaCh12G002520.1.exon.1exon


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004294Carotenoid oxygenasePANTHERPTHR10543BETA-CAROTENE DIOXYGENASEcoord: 9..518
score: 9.4E
IPR004294Carotenoid oxygenasePFAMPF03055RPE65coord: 62..516
score: 9.1
NoneNo IPR availablePANTHERPTHR10543:SF50SUBFAMILY NOT NAMEDcoord: 9..518
score: 9.4E