CmaCh12G000420.1 (mRNA) Cucurbita maxima (Rimu)

NameCmaCh12G000420.1
TypemRNA
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionCysteine proteinase
LocationCma_Chr12 : 161778 .. 163170 (+)
Sequence length1038
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCCATTAGCAAATGCCTTTTGGTTCCATTTCTGATTGTACTAGTTTCAGGATTGGCTAAGAGCTTTGAATTCGATGAGGAGGAGTTAGCAACAGATGGAAGTCTATGGAAGCTCTATGAAAGGTGGAGTCACCACCATGCAATCTCCAGGGAACTAAAGGAGAAGCATAAACGTTACAACGTGTTTAAAGAGAATGCGAACCATGTGCTCACAGTGAATCAAATGAACAAACCATACAAGTTGAAGCTGAACAAATTCGCAGATATGTCCAATTATGAGTTTGTGAACCTGTATGCTCGCTCTAATATTACCCACTACAGGAGGTTACATGGCAGGAGAAGAGAAGGTGCCAGTGGATTCATGTACGAAAAGGCTACAGATCTTCCATCGTTCATTGATTGGAGGGAAAGAGGAGCTGTCAATGACATCAAGTATCAAGGCAGATGTGGTAACTTAGAGCTTACGATGAGGGAAAAAGAAGTTGATTACTAAATCAACATTTAATAGTTTCGAGCGTTGTCTCTATAAGAATATGATAAATTAGAGCTTACGATGTCCATGAAAAGCATTCTTCGACTTTTGAAACCACAAATAACCAATGATTTTGAAATGTATCGCAGGTAGCTGTTGGGCGTTTTCGGCTGTGGCTGCAGTTGAAGGGATCAACCAAATCAAAACCAACCAACTATTGTCTCTATCAGAGCAGGAGCTACTCGACTGCAACACAAGAAACAGAGGCTGCTATGGAGGATTCATGGAAACCGCTTATAATTTCATAAGGCGAAATGGAGGAATCGCCAGCGAGAACAACTATCCCTACCGTGGCGCAAGAGGATCCTGCCGCTCATCTAGAGTAAGTTCTGTAATTGATCTAACATCGGGAAGAAATTCAAACCTAAGATACGGTTCATCCTTCCAGAAATGATCAAACTAAGCATACATTTGTATGGTTTAGCAGATGCCTTCACCAATAGTGACAATAGATGGATTCGAAAGCGTACCTGAAAACGAGAATGCTCTGATGCAAGCCGTCGCAAACCAACCAGTGTCAGTCTCCATCGAGGCCCTAGGGAGAGATTTCCAATTCTACTGGCAGGCAAGTTGTAATAGCTTGTATCACATAGAACCTTCCATCTCTAGGCATTGTTAAATTGGAAGTGTGGGTAATTTGCAGGGAGTGTTCGATGGATATTGTGGAACAGAGCTTAATCACGGAGTGGTGGTGATCGGCTATGGAACAACCGACGGCGGAACAGACTACTGGACTGTGAGGAACTCATGGGGAGTTGGATGGGGAGAGGATGGTTACATAAGGATGAAACGTGGGGTGGAAGATCCAGAAGGTCTGTGTGGAATTGCGATGGAAGCCTCTTACCCCCTCAAGTTCTAA

mRNA sequence

ATGCCCATTAGCAAATGCCTTTTGGTTCCATTTCTGATTGTACTAGTTTCAGGATTGGCTAAGAGCTTTGAATTCGATGAGGAGGAGTTAGCAACAGATGGAAGTCTATGGAAGCTCTATGAAAGGTGGAGTCACCACCATGCAATCTCCAGGGAACTAAAGGAGAAGCATAAACGTTACAACGTGTTTAAAGAGAATGCGAACCATGTGCTCACAGTGAATCAAATGAACAAACCATACAAGTTGAAGCTGAACAAATTCGCAGATATGTCCAATTATGAGTTTGTGAACCTGTATGCTCGCTCTAATATTACCCACTACAGGAGGTTACATGGCAGGAGAAGAGAAGGTGCCAGTGGATTCATGTACGAAAAGGCTACAGATCTTCCATCGTTCATTGATTGGAGGGAAAGAGGAGCTGTCAATGACATCAAGTATCAAGGCAGATGTGGTAGCTGTTGGGCGTTTTCGGCTGTGGCTGCAGTTGAAGGGATCAACCAAATCAAAACCAACCAACTATTGTCTCTATCAGAGCAGGAGCTACTCGACTGCAACACAAGAAACAGAGGCTGCTATGGAGGATTCATGGAAACCGCTTATAATTTCATAAGGCGAAATGGAGGAATCGCCAGCGAGAACAACTATCCCTACCGTGGCGCAAGAGGATCCTGCCGCTCATCTAGAATGCCTTCACCAATAGTGACAATAGATGGATTCGAAAGCGTACCTGAAAACGAGAATGCTCTGATGCAAGCCGTCGCAAACCAACCAGTGTCAGTCTCCATCGAGGCCCTAGGGAGAGATTTCCAATTCTACTGGCAGGGAGTGTTCGATGGATATTGTGGAACAGAGCTTAATCACGGAGTGGTGGTGATCGGCTATGGAACAACCGACGGCGGAACAGACTACTGGACTGTGAGGAACTCATGGGGAGTTGGATGGGGAGAGGATGGTTACATAAGGATGAAACGTGGGGTGGAAGATCCAGAAGGTCTGTGTGGAATTGCGATGGAAGCCTCTTACCCCCTCAAGTTCTAA

Coding sequence (CDS)

ATGCCCATTAGCAAATGCCTTTTGGTTCCATTTCTGATTGTACTAGTTTCAGGATTGGCTAAGAGCTTTGAATTCGATGAGGAGGAGTTAGCAACAGATGGAAGTCTATGGAAGCTCTATGAAAGGTGGAGTCACCACCATGCAATCTCCAGGGAACTAAAGGAGAAGCATAAACGTTACAACGTGTTTAAAGAGAATGCGAACCATGTGCTCACAGTGAATCAAATGAACAAACCATACAAGTTGAAGCTGAACAAATTCGCAGATATGTCCAATTATGAGTTTGTGAACCTGTATGCTCGCTCTAATATTACCCACTACAGGAGGTTACATGGCAGGAGAAGAGAAGGTGCCAGTGGATTCATGTACGAAAAGGCTACAGATCTTCCATCGTTCATTGATTGGAGGGAAAGAGGAGCTGTCAATGACATCAAGTATCAAGGCAGATGTGGTAGCTGTTGGGCGTTTTCGGCTGTGGCTGCAGTTGAAGGGATCAACCAAATCAAAACCAACCAACTATTGTCTCTATCAGAGCAGGAGCTACTCGACTGCAACACAAGAAACAGAGGCTGCTATGGAGGATTCATGGAAACCGCTTATAATTTCATAAGGCGAAATGGAGGAATCGCCAGCGAGAACAACTATCCCTACCGTGGCGCAAGAGGATCCTGCCGCTCATCTAGAATGCCTTCACCAATAGTGACAATAGATGGATTCGAAAGCGTACCTGAAAACGAGAATGCTCTGATGCAAGCCGTCGCAAACCAACCAGTGTCAGTCTCCATCGAGGCCCTAGGGAGAGATTTCCAATTCTACTGGCAGGGAGTGTTCGATGGATATTGTGGAACAGAGCTTAATCACGGAGTGGTGGTGATCGGCTATGGAACAACCGACGGCGGAACAGACTACTGGACTGTGAGGAACTCATGGGGAGTTGGATGGGGAGAGGATGGTTACATAAGGATGAAACGTGGGGTGGAAGATCCAGAAGGTCTGTGTGGAATTGCGATGGAAGCCTCTTACCCCCTCAAGTTCTAA

Protein sequence

MPISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRYNVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASGFMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQELLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFESVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDGGTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLKF
BLAST of CmaCh12G000420.1 vs. Swiss-Prot
Match: CYSEP_RICCO (Vignain OS=Ricinus communis GN=CYSEP PE=1 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 1.3e-121
Identity = 209/344 (60.76%), Postives = 264/344 (76.74%), Query Frame = 1

Query: 3   ISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRYNV 62
           + K +L+   + LV  + +SF+F E+EL ++ SLW LYERW  HH +SR L EK KR+NV
Sbjct: 1   MQKFILLALSLALVLAITESFDFHEKELESEESLWGLYERWRSHHTVSRSLHEKQKRFNV 60

Query: 63  FKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASGFM 122
           FK NA HV   N+M+KPYKLKLNKFADM+N+EF N Y+ S + H+R   G  R G   FM
Sbjct: 61  FKHNAMHVHNANKMDKPYKLKLNKFADMTNHEFRNTYSGSKVKHHRMFRGGPR-GNGTFM 120

Query: 123 YEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQELL 182
           YEK   +P+ +DWR++GAV  +K QG+CGSCWAFS + AVEGINQIKTN+L+SLSEQEL+
Sbjct: 121 YEKVDTVPASVDWRKKGAVTSVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQELV 180

Query: 183 DCNT-RNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFES 242
           DC+T +N+GC GG M+ A+ FI++ GGI +E NYPY    G+C  S+  +P V+IDG E+
Sbjct: 181 DCDTDQNQGCNGGLMDYAFEFIKQRGGITTEANYPYEAYDGTCDVSKENAPAVSIDGHEN 240

Query: 243 VPEN-ENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDGG 302
           VPEN ENAL++AVANQPVSV+I+A G DFQFY +GVF G CGTEL+HGV ++GYGTT  G
Sbjct: 241 VPENDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGSCGTELDHGVAIVGYGTTIDG 300

Query: 303 TDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLK 345
           T YWTV+NSWG  WGE GYIRM+RG+ D EGLCGIAMEASYP+K
Sbjct: 301 TKYWTVKNSWGPEWGEKGYIRMERGISDKEGLCGIAMEASYPIK 343

BLAST of CmaCh12G000420.1 vs. Swiss-Prot
Match: CYSEP_VIGMU (Vignain OS=Vigna mungo PE=1 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 4.6e-119
Identity = 205/346 (59.25%), Postives = 261/346 (75.43%), Query Frame = 1

Query: 1   MPISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY 60
           M + K L V   + LV G+A SF+F E++L ++ SLW LYERW  HH +SR L EKHKR+
Sbjct: 1   MAMKKLLWVVLSLSLVLGVANSFDFHEKDLESEESLWDLYERWRSHHTVSRSLGEKHKRF 60

Query: 61  NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASG 120
           NVFK N  HV   N+M+KPYKLKLNKFADM+N+EF + YA S + H++   G +  G+  
Sbjct: 61  NVFKANVMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHHKMFRGSQH-GSGT 120

Query: 121 FMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 180
           FMYEK   +P+ +DWR++GAV D+K QG+CGSCWAFS + AVEGINQIKTN+L+SLSEQE
Sbjct: 121 FMYEKVGSVPASVDWRKKGAVTDVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQE 180

Query: 181 LLDCNTR-NRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGF 240
           L+DC+   N+GC GG ME+A+ FI++ GGI +E+NYPY    G+C  S++    V+IDG 
Sbjct: 181 LVDCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYTAQEGTCDESKVNDLAVSIDGH 240

Query: 241 ESVPEN-ENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTD 300
           E+VP N ENAL++AVANQPVSV+I+A G DFQFY +GVF G C T+LNHGV ++GYGTT 
Sbjct: 241 ENVPVNDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGDCNTDLNHGVAIVGYGTTV 300

Query: 301 GGTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLK 345
            GT+YW VRNSWG  WGE GYIRM+R +   EGLCGIAM ASYP+K
Sbjct: 301 DGTNYWIVRNSWGPEWGEQGYIRMQRNISKKEGLCGIAMMASYPIK 345

BLAST of CmaCh12G000420.1 vs. Swiss-Prot
Match: CYSEP_PHAVU (Vignain OS=Phaseolus vulgaris PE=2 SV=2)

HSP 1 Score: 423.3 bits (1087), Expect = 2.5e-117
Identity = 204/346 (58.96%), Postives = 258/346 (74.57%), Query Frame = 1

Query: 1   MPISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY 60
           M   K L V     LV G+A SF+F +++LA++ SLW LYERW  HH +SR L EKHKR+
Sbjct: 1   MATKKLLWVVLSFSLVLGVANSFDFHDKDLASEESLWDLYERWRSHHTVSRSLGEKHKRF 60

Query: 61  NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASG 120
           NVFK N  HV   N+M+KPYKLKLNKFADM+N+EF + YA S + H R   G   E  + 
Sbjct: 61  NVFKANLMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHPRMFRGTPHENGA- 120

Query: 121 FMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 180
           FMYEK   +P  +DWR++GAV D+K QG+CGSCWAFS V AVEGINQIKTN+L++LSEQE
Sbjct: 121 FMYEKVVSVPPSVDWRKKGAVTDVKDQGQCGSCWAFSTVVAVEGINQIKTNKLVALSEQE 180

Query: 181 LLDCNTR-NRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGF 240
           L+DC+   N+GC GG ME+A+ FI++ GGI +E+NYPY+   G+C +S++    V+IDG 
Sbjct: 181 LVDCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYKAQEGTCDASKVNDLAVSIDGH 240

Query: 241 ESVPEN-ENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTD 300
           E+VP N E+AL++AVANQPVSV+I+A G DFQFY +GVF G C T+LNHGV ++GYGTT 
Sbjct: 241 ENVPANDEDALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGDCSTDLNHGVAIVGYGTTV 300

Query: 301 GGTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLK 345
            GT+YW VRNSWG  WGE GYIRM+R +   EGLCGIAM  SYP+K
Sbjct: 301 DGTNYWIVRNSWGPEWGEHGYIRMQRNISKKEGLCGIAMLPSYPIK 345

BLAST of CmaCh12G000420.1 vs. Swiss-Prot
Match: CEP3_ARATH (KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana GN=CEP3 PE=2 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 4.2e-112
Identity = 199/342 (58.19%), Postives = 255/342 (74.56%), Query Frame = 1

Query: 11  FLIVLVSGLA-----KSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRYNVFKE 70
           F IVL+S L+     K F+FDE+EL T+ ++WKLYERW  HH++SR   E  KR+NVF+ 
Sbjct: 4   FFIVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNVFRH 63

Query: 71  NANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASGFMYEK 130
           N  HV   N+ NKPYKLK+N+FAD++++EF + YA SN+ H+R L G +R G+ GFMYE 
Sbjct: 64  NVLHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKR-GSGGFMYEN 123

Query: 131 ATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQELLDCN 190
            T +PS +DWRE+GAV ++K Q  CGSCWAFS VAAVEGIN+I+TN+L+SLSEQEL+DC+
Sbjct: 124 VTRVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQELVDCD 183

Query: 191 TR-NRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGS-CRSSRMPSPIVTIDGFESVP 250
           T  N+GC GG ME A+ FI+ NGGI +E  YPY  +    CR++ +    VTIDG E VP
Sbjct: 184 TEENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDGHEHVP 243

Query: 251 EN-ENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDGGTD 310
           EN E  L++AVA+QPVSV+I+A   DFQ Y +GVF G CGT+LNHGVV++GYG T  GT 
Sbjct: 244 ENDEEELLKAVAHQPVSVAIDAGSSDFQLYSEGVFIGECGTQLNHGVVIVGYGETKNGTK 303

Query: 311 YWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLK 345
           YW VRNSWG  WGE GY+R++RG+ + EG CGIAMEASYP K
Sbjct: 304 YWIVRNSWGPEWGEGGYVRIERGISENEGRCGIAMEASYPTK 344

BLAST of CmaCh12G000420.1 vs. Swiss-Prot
Match: CEP2_ARATH (KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana GN=CEP2 PE=1 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 4.6e-111
Identity = 196/346 (56.65%), Postives = 257/346 (74.28%), Query Frame = 1

Query: 3   ISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRYNV 62
           + K LL+    +++   A  F++D++E+ ++  L  LY+RW  HH++ R L E+ KR+NV
Sbjct: 1   MKKLLLIFLFSLVILQTACGFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFNV 60

Query: 63  FKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASGFM 122
           F+ N  HV   N+ N+ YKLKLNKFAD++  EF N Y  SNI H+R L G +R G+  FM
Sbjct: 61  FRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKR-GSKQFM 120

Query: 123 Y--EKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 182
           Y  E  + LPS +DWR++GAV +IK QG+CGSCWAFS VAAVEGIN+IKTN+L+SLSEQE
Sbjct: 121 YDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQE 180

Query: 183 LLDCNTR-NRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGF 242
           L+DC+T+ N GC GG ME A+ FI++NGGI +E++YPY G  G C +S+    +VTIDG 
Sbjct: 181 LVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDGH 240

Query: 243 ESVPEN-ENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTD 302
           E VPEN ENAL++AVANQPVSV+I+A   DFQFY +GVF G CGTELNHGV  +GYG ++
Sbjct: 241 EDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSEGVFTGSCGTELNHGVAAVGYG-SE 300

Query: 303 GGTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLK 345
            G  YW VRNSWG  WGE GYI+++R +++PEG CGIAMEASYP+K
Sbjct: 301 RGKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

BLAST of CmaCh12G000420.1 vs. TrEMBL
Match: A0A0A0LMU4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G349680 PE=3 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 2.5e-164
Identity = 282/346 (81.50%), Postives = 312/346 (90.17%), Query Frame = 1

Query: 1   MPISKCLLVPFL-IVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKR 60
           M I K LLVP L IVLVSGLA+SFEFDE+ELAT+ SLW+LYERW  HH ISR LKEKHKR
Sbjct: 1   MAIGKFLLVPLLLIVLVSGLAESFEFDEKELATEESLWQLYERWGKHHTISRNLKEKHKR 60

Query: 61  YNVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGAS 120
           ++VFKEN NHV TVNQM+KPYKLKLNKFADMSNYEFVN YARSNI+HYR+LH RRR GA 
Sbjct: 61  FSVFKENVNHVFTVNQMDKPYKLKLNKFADMSNYEFVNFYARSNISHYRKLHERRR-GAG 120

Query: 121 GFMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQ 180
           GFMYE+ TDLPS +DWRERGAVN +K QGRCGSCWAFS+VAAVEGIN+IKTNQLLSLSEQ
Sbjct: 121 GFMYEQDTDLPSSVDWRERGAVNAVKEQGRCGSCWAFSSVAAVEGINKIKTNQLLSLSEQ 180

Query: 181 ELLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGF 240
           ELLDCN RN+GC GGFME A++FI+RNGGIA+EN+YPY G+RG CRSSR+ SPIV IDG+
Sbjct: 181 ELLDCNYRNKGCNGGFMEIAFDFIKRNGGIATENSYPYHGSRGLCRSSRISSPIVKIDGY 240

Query: 241 ESVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDG 300
           ESVPENE+ALMQAVANQPVSV+I+A GRDFQFY QGVFDGYCGTELNHGVV IGYGTT+ 
Sbjct: 241 ESVPENEDALMQAVANQPVSVAIDAAGRDFQFYSQGVFDGYCGTELNHGVVAIGYGTTED 300

Query: 301 GTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLKF 346
           GTDYW VRNSWGVGWGEDGY+RMKRGVE  EGLCGIAMEASYP+K+
Sbjct: 301 GTDYWLVRNSWGVGWGEDGYVRMKRGVEQAEGLCGIAMEASYPIKY 345

BLAST of CmaCh12G000420.1 vs. TrEMBL
Match: W9RKI3_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_027645 PE=3 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 1.5e-124
Identity = 217/346 (62.72%), Postives = 270/346 (78.03%), Query Frame = 1

Query: 1   MPISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY 60
           M + K  L    +VL+ GLA+SFEF EE+LA++  LW LYERW   H +SR+LKEKH+R+
Sbjct: 1   MELGKFFLAALSLVLLLGLAQSFEFHEEDLASEERLWDLYERWRSQHTVSRDLKEKHQRF 60

Query: 61  NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASG 120
           NVFK NA HV  VNQMNKPYKL+LNKFADM+N+EFV  YA S ++HYR   G +   A+ 
Sbjct: 61  NVFKANAKHVHKVNQMNKPYKLRLNKFADMTNHEFVRSYAGSKVSHYRMFRGEKP--ATD 120

Query: 121 FMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 180
           F + K  DLP+ +DWR++GAV  IK QG CGSCWAFSAV AVEG+NQIKT +L+ LSEQE
Sbjct: 121 FSHGKTEDLPTSVDWRKKGAVTGIKDQGNCGSCWAFSAVVAVEGVNQIKTKELMPLSEQE 180

Query: 181 LLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMP-SPIVTIDGF 240
           L+DCN++N GC GG M+ A+ FI+++GGI +E NYPY+   G+C SSR+  +P+V IDG+
Sbjct: 181 LVDCNSKNNGCDGGLMQDAFEFIKQHGGITTEKNYPYQARDGTCDSSRVTNAPLVVIDGY 240

Query: 241 ESVPEN-ENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTD 300
           E VPEN ENALM+AVANQPVSVSI+A G+DFQFY +GV+ G CGTELNHGV ++GYG T 
Sbjct: 241 EMVPENDENALMKAVANQPVSVSIDAGGKDFQFYSEGVYTGSCGTELNHGVAIVGYGATL 300

Query: 301 GGTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLK 345
            GT YW V+NSWG  WGE GY+R++RGVE  EGLCGIAMEASYP+K
Sbjct: 301 DGTKYWLVKNSWGTEWGERGYLRIQRGVEAEEGLCGIAMEASYPVK 344

BLAST of CmaCh12G000420.1 vs. TrEMBL
Match: D7SME9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0137g00330 PE=3 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 1.3e-123
Identity = 218/346 (63.01%), Postives = 270/346 (78.03%), Query Frame = 1

Query: 1   MPISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY 60
           M + K +LV   +VLV GLA+SF+FDE++LA++ SLW LYERW  +H +SR+L+EK+KR+
Sbjct: 1   MKMEKVILVALSLVLVFGLAESFDFDEKDLASEESLWDLYERWRSYHTVSRDLEEKNKRF 60

Query: 61  NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASG 120
           NVFKEN  HV  VNQM+KPYKLKLNKFADM+N+EF + Y  S + HYR L G RR G  G
Sbjct: 61  NVFKENTKHVHKVNQMDKPYKLKLNKFADMTNHEFRSSYGGSKVKHYRMLRGDRR-GTGG 120

Query: 121 FMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 180
           FM+EK T LP  +DWR++GAV  IK QG+CGSCWAFS V  VEGINQIKT +LLSLSEQ+
Sbjct: 121 FMHEKTTYLPPSVDWRKKGAVTGIKDQGKCGSCWAFSTVVGVEGINQIKTKELLSLSEQQ 180

Query: 181 LLDCN-TRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGF 240
           L+DC+ + + GC GG ME+A+ FI++NGGI +ENNYPY+     C   +M +P+VTIDG 
Sbjct: 181 LIDCDRSDDHGCNGGLMESAFEFIKKNGGITTENNYPYKAKDERCDMLKMNAPVVTIDGH 240

Query: 241 ESVPEN-ENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTD 300
           ESVP N E ALM+AVA+QPVSV+I+A G D QFY +GVFDG CGTEL+HGV ++GYGTT 
Sbjct: 241 ESVPVNDERALMKAVAHQPVSVAIDAGGSDLQFYSEGVFDGECGTELDHGVAIVGYGTTL 300

Query: 301 GGTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLK 345
            GT YW V+NSWG  WGE GYIRM RG++  EG CGIAMEASYP+K
Sbjct: 301 DGTKYWIVKNSWGAEWGEKGYIRMARGIQAAEGQCGIAMEASYPVK 345

BLAST of CmaCh12G000420.1 vs. TrEMBL
Match: M5XQB2_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa025615mg PE=3 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 2.8e-123
Identity = 221/343 (64.43%), Postives = 268/343 (78.13%), Query Frame = 1

Query: 5   KCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRYNVFK 64
           K +LV   + LV GLA+SFEF E++LA++ SLW LYE W  HH IS +L EK KR+NVFK
Sbjct: 3   KFILVALFLALVIGLAESFEFQEKDLASEESLWGLYEGWRSHHTISHDLGEKEKRFNVFK 62

Query: 65  ENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASGFMYE 124
           EN  HV  VNQM+KPYKLKLNKFADM+N+EFV+ YA S ++HYR LHG RRE A  F +E
Sbjct: 63  ENVKHVHKVNQMSKPYKLKLNKFADMTNHEFVSSYAGSKVSHYRSLHGSRRETA--FTHE 122

Query: 125 KATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQELLDC 184
              +LP  +DWR+ GAV  +K QG+CGSCWAFS V AVEGINQIKT  L+SLSEQEL+DC
Sbjct: 123 NTDNLPPNVDWRKNGAVTGVKDQGKCGSCWAFSTVVAVEGINQIKTKALVSLSEQELVDC 182

Query: 185 NTR-NRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPS-PIVTIDGFESV 244
           N   N GC GG ME A++FI++NGGI +E NYPYR + G C S++M + P+V IDG+E+V
Sbjct: 183 NRDPNEGCDGGLMEKAFDFIKKNGGITTEQNYPYRASDGPCDSTKMMNAPLVQIDGYENV 242

Query: 245 PE-NENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDGGT 304
           PE NENALM+AVANQPVSV+I+A GRDFQFY +GVF+G CGTELNHGV V+GYG T  GT
Sbjct: 243 PENNENALMKAVANQPVSVAIDAGGRDFQFYSEGVFNGDCGTELNHGVAVVGYGATLDGT 302

Query: 305 DYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLK 345
            YW V+NSWG  WGE GYIR++RGV+  EGLCGIA + SYP+K
Sbjct: 303 KYWIVKNSWGEEWGEKGYIRIQRGVDAEEGLCGIAKDPSYPMK 343

BLAST of CmaCh12G000420.1 vs. TrEMBL
Match: B9RMS9_RICCO (Cysteine protease, putative OS=Ricinus communis GN=RCOM_1083340 PE=3 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 3.1e-122
Identity = 208/345 (60.29%), Postives = 272/345 (78.84%), Query Frame = 1

Query: 1   MPISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY 60
           M   K +L+   +VLV  +A+SF++ EE+LA++ SLW LYERW  HH +SR L EK++R+
Sbjct: 1   MEARKIILLALSLVLVLKVARSFDYKEEDLASEESLWNLYERWRSHHTVSRSLTEKNQRF 60

Query: 61  NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASG 120
           NVFKEN  H+  VNQ ++PYKL+LNKFADM+N+EF+  Y  S ++HYR  HG RR+  +G
Sbjct: 61  NVFKENLKHIHKVNQKDRPYKLRLNKFADMTNHEFLQHYGGSKVSHYRMFHGSRRQ--TG 120

Query: 121 FMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 180
           F +E  ++LPS IDWR++GAV  +K QG+CGSCWAFS+VAAVEGIN+IKT +L+SLSEQE
Sbjct: 121 FAHENTSNLPSSIDWRKQGAVTGVKDQGKCGSCWAFSSVAAVEGINKIKTGELISLSEQE 180

Query: 181 LLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFE 240
           L+DCN+ N GC GG ME A++FI + GG+ +ENNYPYR   G C S++M +P+VTIDG+E
Sbjct: 181 LVDCNSVNHGCDGGLMEQAFSFIEKTGGLTTENNYPYRAKDGYCDSAKMNTPMVTIDGYE 240

Query: 241 SVPEN-ENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDG 300
            VPEN E+ALMQAVANQPVS++I+A G+DFQFY +GV+ G CGTELNHGV ++GYG T  
Sbjct: 241 MVPENDEHALMQAVANQPVSIAIDAGGQDFQFYSEGVYTGDCGTELNHGVALVGYGATQD 300

Query: 301 GTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLK 345
           GT YW V+NSWG  WGE+G+IRM+R  +  EGLCGI +EASYP+K
Sbjct: 301 GTKYWIVKNSWGSEWGENGFIRMQRENDVEEGLCGITLEASYPIK 343

BLAST of CmaCh12G000420.1 vs. TAIR10
Match: AT3G48350.1 (AT3G48350.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 406.0 bits (1042), Expect = 2.3e-113
Identity = 199/342 (58.19%), Postives = 255/342 (74.56%), Query Frame = 1

Query: 11  FLIVLVSGLA-----KSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRYNVFKE 70
           F IVL+S L+     K F+FDE+EL T+ ++WKLYERW  HH++SR   E  KR+NVF+ 
Sbjct: 4   FFIVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNVFRH 63

Query: 71  NANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASGFMYEK 130
           N  HV   N+ NKPYKLK+N+FAD++++EF + YA SN+ H+R L G +R G+ GFMYE 
Sbjct: 64  NVLHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKR-GSGGFMYEN 123

Query: 131 ATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQELLDCN 190
            T +PS +DWRE+GAV ++K Q  CGSCWAFS VAAVEGIN+I+TN+L+SLSEQEL+DC+
Sbjct: 124 VTRVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQELVDCD 183

Query: 191 TR-NRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGS-CRSSRMPSPIVTIDGFESVP 250
           T  N+GC GG ME A+ FI+ NGGI +E  YPY  +    CR++ +    VTIDG E VP
Sbjct: 184 TEENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDGHEHVP 243

Query: 251 EN-ENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDGGTD 310
           EN E  L++AVA+QPVSV+I+A   DFQ Y +GVF G CGT+LNHGVV++GYG T  GT 
Sbjct: 244 ENDEEELLKAVAHQPVSVAIDAGSSDFQLYSEGVFIGECGTQLNHGVVIVGYGETKNGTK 303

Query: 311 YWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLK 345
           YW VRNSWG  WGE GY+R++RG+ + EG CGIAMEASYP K
Sbjct: 304 YWIVRNSWGPEWGEGGYVRIERGISENEGRCGIAMEASYPTK 344

BLAST of CmaCh12G000420.1 vs. TAIR10
Match: AT3G48340.1 (AT3G48340.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 402.5 bits (1033), Expect = 2.6e-112
Identity = 196/346 (56.65%), Postives = 257/346 (74.28%), Query Frame = 1

Query: 3   ISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRYNV 62
           + K LL+    +++   A  F++D++E+ ++  L  LY+RW  HH++ R L E+ KR+NV
Sbjct: 1   MKKLLLIFLFSLVILQTACGFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFNV 60

Query: 63  FKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASGFM 122
           F+ N  HV   N+ N+ YKLKLNKFAD++  EF N Y  SNI H+R L G +R G+  FM
Sbjct: 61  FRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKR-GSKQFM 120

Query: 123 Y--EKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 182
           Y  E  + LPS +DWR++GAV +IK QG+CGSCWAFS VAAVEGIN+IKTN+L+SLSEQE
Sbjct: 121 YDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQE 180

Query: 183 LLDCNTR-NRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGF 242
           L+DC+T+ N GC GG ME A+ FI++NGGI +E++YPY G  G C +S+    +VTIDG 
Sbjct: 181 LVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDGH 240

Query: 243 ESVPEN-ENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTD 302
           E VPEN ENAL++AVANQPVSV+I+A   DFQFY +GVF G CGTELNHGV  +GYG ++
Sbjct: 241 EDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSEGVFTGSCGTELNHGVAAVGYG-SE 300

Query: 303 GGTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLK 345
            G  YW VRNSWG  WGE GYI+++R +++PEG CGIAMEASYP+K
Sbjct: 301 RGKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

BLAST of CmaCh12G000420.1 vs. TAIR10
Match: AT5G50260.1 (AT5G50260.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 399.8 bits (1026), Expect = 1.7e-111
Identity = 190/344 (55.23%), Postives = 251/344 (72.97%), Query Frame = 1

Query: 3   ISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRYNV 62
           + + +++   +++V    K  +F  +++ ++ SLW+LYERW  HH ++R L+EK KR+NV
Sbjct: 1   MKRFIVLALCMLMVLETTKGLDFHNKDVESENSLWELYERWRSHHTVARSLEEKAKRFNV 60

Query: 63  FKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASGFM 122
           FK N  H+   N+ +K YKLKLNKF DM++ EF   YA SNI H+R   G ++   S FM
Sbjct: 61  FKHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRTYAGSNIKHHRMFQGEKKATKS-FM 120

Query: 123 YEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQELL 182
           Y     LP+ +DWR+ GAV  +K QG+CGSCWAFS V AVEGINQI+T +L SLSEQEL+
Sbjct: 121 YANVNTLPTSVDWRKNGAVTPVKNQGQCGSCWAFSTVVAVEGINQIRTKKLTSLSEQELV 180

Query: 183 DCNT-RNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFES 242
           DC+T +N+GC GG M+ A+ FI+  GG+ SE  YPY+ +  +C +++  +P+V+IDG E 
Sbjct: 181 DCDTNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPYKASDETCDTNKENAPVVSIDGHED 240

Query: 243 VPEN-ENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDGG 302
           VP+N E+ LM+AVANQPVSV+I+A G DFQFY +GVF G CGTELNHGV V+GYGTT  G
Sbjct: 241 VPKNSEDDLMKAVANQPVSVAIDAGGSDFQFYSEGVFTGRCGTELNHGVAVVGYGTTIDG 300

Query: 303 TDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLK 345
           T YW V+NSWG  WGE GYIRM+RG+   EGLCGIAMEASYPLK
Sbjct: 301 TKYWIVKNSWGEEWGEKGYIRMQRGIRHKEGLCGIAMEASYPLK 343

BLAST of CmaCh12G000420.1 vs. TAIR10
Match: AT5G43060.1 (AT5G43060.1 Granulin repeat cysteine protease family protein)

HSP 1 Score: 315.5 bits (807), Expect = 4.2e-86
Identity = 161/324 (49.69%), Postives = 213/324 (65.74%), Query Frame = 1

Query: 28  EELATDGSLWKLYERWSHHHAISRELK-----EKHKRYNVFKENANHVLTVNQMNKPYKL 87
           E   +D  + ++YE W   H   +  +     EK +R+ +FK+N   +   N  N  YKL
Sbjct: 38  ETSRSDSEVERIYEAWMVEHGKKKMNQNGLGAEKDQRFEIFKDNLRFIDEHNTKNLSYKL 97

Query: 88  KLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASGFMYEKATDLPSFIDWRERGAVN 147
            L +FAD++N E+ ++Y  +  T       R  + +  +       LP  +DWR+ GAV 
Sbjct: 98  GLTRFADLTNEEYRSMYLGAKPTK------RVLKTSDRYQARVGDALPDSVDWRKEGAVA 157

Query: 148 DIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQELLDCNTR-NRGCYGGFMETAYN 207
           D+K QG CGSCWAFS + AVEGIN+I T  L+SLSEQEL+DC+T  N+GC GG M+ A+ 
Sbjct: 158 DVKDQGSCGSCWAFSTIGAVEGINKIVTGDLISLSEQELVDCDTSYNQGCNGGLMDYAFE 217

Query: 208 FIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFESVPEN-ENALMQAVANQPVSV 267
           FI +NGGI +E +YPY+ A G C  +R  + +VTID +E VPEN E +L +A+A+QP+SV
Sbjct: 218 FIIKNGGIDTEADYPYKAADGRCDQNRKNAKVVTIDSYEDVPENSEASLKKALAHQPISV 277

Query: 268 SIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDGGTDYWTVRNSWGVGWGEDGYI 327
           +IEA GR FQ Y  GVFDG CGTEL+HGVV +GYG T+ G DYW VRNSWG  WGE GYI
Sbjct: 278 AIEAGGRAFQLYSSGVFDGLCGTELDHGVVAVGYG-TENGKDYWIVRNSWGNRWGESGYI 337

Query: 328 RMKRGVEDPEGLCGIAMEASYPLK 345
           +M R +E P G CGIAMEASYP+K
Sbjct: 338 KMARNIEAPTGKCGIAMEASYPIK 354

BLAST of CmaCh12G000420.1 vs. TAIR10
Match: AT4G35350.1 (AT4G35350.1 xylem cysteine peptidase 1)

HSP 1 Score: 308.1 bits (788), Expect = 6.6e-84
Identity = 163/337 (48.37%), Postives = 216/337 (64.09%), Query Frame = 1

Query: 14  VLVSGLAKSFE---FDEEELATDGSLWKLYERW-SHHHAISRELKEKHKRYNVFKENANH 73
           +L    A+ F    +  E L     L +L+E W S H    + ++EK  R+ VF+EN  H
Sbjct: 22  LLCCAFARDFSIVGYTPEHLTNTDKLLELFESWMSEHSKAYKSVEEKVHRFEVFRENLMH 81

Query: 74  VLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASGFMYEKATDL 133
           +   N     Y L LN+FAD+++ EF   Y       +     R+R+ ++ F Y   TDL
Sbjct: 82  IDQRNNEINSYWLGLNEFADLTHEEFKGRYLGLAKPQF----SRKRQPSANFRYRDITDL 141

Query: 134 PSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQELLDCNTR-N 193
           P  +DWR++GAV  +K QG+CGSCWAFS VAAVEGINQI T  L SLSEQEL+DC+T  N
Sbjct: 142 PKSVDWRKKGAVAPVKDQGQCGSCWAFSTVAAVEGINQITTGNLSSLSEQELIDCDTTFN 201

Query: 194 RGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFESVPENEN- 253
            GC GG M+ A+ +I   GG+  E++YPY    G C+  +     VTI G+E VPEN++ 
Sbjct: 202 SGCNGGLMDYAFQYIISTGGLHKEDDYPYLMEEGICQEQKEDVERVTISGYEDVPENDDE 261

Query: 254 ALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDGGTDYWTVR 313
           +L++A+A+QPVSV+IEA GRDFQFY  GVF+G CGT+L+HGV  +GYG++  G+DY  V+
Sbjct: 262 SLVKALAHQPVSVAIEASGRDFQFYKGGVFNGKCGTDLDHGVAAVGYGSSK-GSDYVIVK 321

Query: 314 NSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLK 345
           NSWG  WGE G+IRMKR    PEGLCGI   ASYP K
Sbjct: 322 NSWGPRWGEKGFIRMKRNTGKPEGLCGINKMASYPTK 353

BLAST of CmaCh12G000420.1 vs. NCBI nr
Match: gi|659087323|ref|XP_008444390.1| (PREDICTED: vignain-like [Cucumis melo])

HSP 1 Score: 595.9 bits (1535), Expect = 4.5e-167
Identity = 285/346 (82.37%), Postives = 314/346 (90.75%), Query Frame = 1

Query: 1   MPISKCLLVPFL-IVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKR 60
           M I K LLVP L IVLVSGLA+SFEFDE+ELAT+ SLW+LYERW +HH ISR LKEKHKR
Sbjct: 1   MAIGKFLLVPLLLIVLVSGLAESFEFDEKELATEESLWQLYERWGNHHTISRNLKEKHKR 60

Query: 61  YNVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGAS 120
           ++VFKEN NHV TVNQMNKPYKLKLNKFADMSNYEFVN YARSNI+H+R+LHGRRR GA 
Sbjct: 61  FSVFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNFYARSNISHFRKLHGRRR-GAG 120

Query: 121 GFMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQ 180
           GFMYE+ TDLPS +DWRERGAVN IK QG CGSCWAFS+VAAVE IN+IKTNQLLSLSEQ
Sbjct: 121 GFMYEQDTDLPSSVDWRERGAVNAIKEQGTCGSCWAFSSVAAVEAINKIKTNQLLSLSEQ 180

Query: 181 ELLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGF 240
           ELLDCN RN+GC GGFME A++FI+RNGGIA+EN+YPY G+RG CRSSR+ SPIV IDG+
Sbjct: 181 ELLDCNYRNKGCNGGFMEIAFDFIKRNGGIATENSYPYHGSRGLCRSSRISSPIVKIDGY 240

Query: 241 ESVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDG 300
           ESVPENE+ALMQAVANQPVSV+I+A GRDFQFYWQGVFDGYCGTELNHGVV IGYGTT+ 
Sbjct: 241 ESVPENEDALMQAVANQPVSVAIDAAGRDFQFYWQGVFDGYCGTELNHGVVAIGYGTTED 300

Query: 301 GTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLKF 346
           GTDYW VRNSWGVGWGEDGY+RMKRGVE PEGLCGIAMEASYP+KF
Sbjct: 301 GTDYWIVRNSWGVGWGEDGYVRMKRGVEQPEGLCGIAMEASYPIKF 345

BLAST of CmaCh12G000420.1 vs. NCBI nr
Match: gi|449450419|ref|XP_004142960.1| (PREDICTED: vignain-like [Cucumis sativus])

HSP 1 Score: 586.3 bits (1510), Expect = 3.6e-164
Identity = 282/346 (81.50%), Postives = 312/346 (90.17%), Query Frame = 1

Query: 1   MPISKCLLVPFL-IVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKR 60
           M I K LLVP L IVLVSGLA+SFEFDE+ELAT+ SLW+LYERW  HH ISR LKEKHKR
Sbjct: 1   MAIGKFLLVPLLLIVLVSGLAESFEFDEKELATEESLWQLYERWGKHHTISRNLKEKHKR 60

Query: 61  YNVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGAS 120
           ++VFKEN NHV TVNQM+KPYKLKLNKFADMSNYEFVN YARSNI+HYR+LH RRR GA 
Sbjct: 61  FSVFKENVNHVFTVNQMDKPYKLKLNKFADMSNYEFVNFYARSNISHYRKLHERRR-GAG 120

Query: 121 GFMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQ 180
           GFMYE+ TDLPS +DWRERGAVN +K QGRCGSCWAFS+VAAVEGIN+IKTNQLLSLSEQ
Sbjct: 121 GFMYEQDTDLPSSVDWRERGAVNAVKEQGRCGSCWAFSSVAAVEGINKIKTNQLLSLSEQ 180

Query: 181 ELLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGF 240
           ELLDCN RN+GC GGFME A++FI+RNGGIA+EN+YPY G+RG CRSSR+ SPIV IDG+
Sbjct: 181 ELLDCNYRNKGCNGGFMEIAFDFIKRNGGIATENSYPYHGSRGLCRSSRISSPIVKIDGY 240

Query: 241 ESVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDG 300
           ESVPENE+ALMQAVANQPVSV+I+A GRDFQFY QGVFDGYCGTELNHGVV IGYGTT+ 
Sbjct: 241 ESVPENEDALMQAVANQPVSVAIDAAGRDFQFYSQGVFDGYCGTELNHGVVAIGYGTTED 300

Query: 301 GTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLKF 346
           GTDYW VRNSWGVGWGEDGY+RMKRGVE  EGLCGIAMEASYP+K+
Sbjct: 301 GTDYWLVRNSWGVGWGEDGYVRMKRGVEQAEGLCGIAMEASYPIKY 345

BLAST of CmaCh12G000420.1 vs. NCBI nr
Match: gi|703113247|ref|XP_010100336.1| (hypothetical protein L484_027645 [Morus notabilis])

HSP 1 Score: 454.1 bits (1167), Expect = 2.1e-124
Identity = 217/346 (62.72%), Postives = 270/346 (78.03%), Query Frame = 1

Query: 1   MPISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY 60
           M + K  L    +VL+ GLA+SFEF EE+LA++  LW LYERW   H +SR+LKEKH+R+
Sbjct: 1   MELGKFFLAALSLVLLLGLAQSFEFHEEDLASEERLWDLYERWRSQHTVSRDLKEKHQRF 60

Query: 61  NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASG 120
           NVFK NA HV  VNQMNKPYKL+LNKFADM+N+EFV  YA S ++HYR   G +   A+ 
Sbjct: 61  NVFKANAKHVHKVNQMNKPYKLRLNKFADMTNHEFVRSYAGSKVSHYRMFRGEKP--ATD 120

Query: 121 FMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 180
           F + K  DLP+ +DWR++GAV  IK QG CGSCWAFSAV AVEG+NQIKT +L+ LSEQE
Sbjct: 121 FSHGKTEDLPTSVDWRKKGAVTGIKDQGNCGSCWAFSAVVAVEGVNQIKTKELMPLSEQE 180

Query: 181 LLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMP-SPIVTIDGF 240
           L+DCN++N GC GG M+ A+ FI+++GGI +E NYPY+   G+C SSR+  +P+V IDG+
Sbjct: 181 LVDCNSKNNGCDGGLMQDAFEFIKQHGGITTEKNYPYQARDGTCDSSRVTNAPLVVIDGY 240

Query: 241 ESVPEN-ENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTD 300
           E VPEN ENALM+AVANQPVSVSI+A G+DFQFY +GV+ G CGTELNHGV ++GYG T 
Sbjct: 241 EMVPENDENALMKAVANQPVSVSIDAGGKDFQFYSEGVYTGSCGTELNHGVAIVGYGATL 300

Query: 301 GGTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLK 345
            GT YW V+NSWG  WGE GY+R++RGVE  EGLCGIAMEASYP+K
Sbjct: 301 DGTKYWLVKNSWGTEWGERGYLRIQRGVEAEEGLCGIAMEASYPVK 344

BLAST of CmaCh12G000420.1 vs. NCBI nr
Match: gi|1009153182|ref|XP_015894499.1| (PREDICTED: vignain-like [Ziziphus jujuba])

HSP 1 Score: 454.1 bits (1167), Expect = 2.1e-124
Identity = 217/346 (62.72%), Postives = 273/346 (78.90%), Query Frame = 1

Query: 1   MPISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY 60
           M + K  LV F + LV GLA+S +  +++LA++ +LW LYERW   H +S++LKEKH R+
Sbjct: 1   MDMRKFFLVAFSLALVLGLAESIDIHDKDLASEETLWDLYERWRSQHTVSKDLKEKHTRF 60

Query: 61  NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASG 120
           NVFK NA H+  VN+MNKPYKLKLNKFADM+N+EFV+ YA S ++HYR LHG R+  A+ 
Sbjct: 61  NVFKMNAKHIHKVNRMNKPYKLKLNKFADMTNHEFVSSYAGSKVSHYRMLHGDRK--ATC 120

Query: 121 FMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 180
           F +E+  +LP  +DWR++GAV  +K QGRCGSCWAFS V AVEGINQI+T +L+SLSEQE
Sbjct: 121 FRHEETNNLPPSVDWRKKGAVTGVKDQGRCGSCWAFSTVVAVEGINQIETKELVSLSEQE 180

Query: 181 LLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMP-SPIVTIDGF 240
           L+DCN  N+GC GG META+ FI++NGGI +EN+YPY    GSC SSR+  SP+V IDG 
Sbjct: 181 LVDCNKDNQGCNGGLMETAFEFIKQNGGITTENSYPYTAKDGSCDSSRITNSPLVIIDGH 240

Query: 241 ESVPEN-ENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTD 300
           E VPEN ENALM+AVANQPVSV+++A G+DFQFY +GVF G CGTELNHGV ++GYG T 
Sbjct: 241 EMVPENDENALMKAVANQPVSVALDAGGKDFQFYSEGVFTGDCGTELNHGVAIVGYGATL 300

Query: 301 GGTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLK 345
            GT YW V+NSWG  WGE GYIR++RG++  EGLCGIAMEASYP+K
Sbjct: 301 DGTKYWIVKNSWGAEWGEKGYIRIQRGIDAEEGLCGIAMEASYPIK 344

BLAST of CmaCh12G000420.1 vs. NCBI nr
Match: gi|731372760|ref|XP_002285397.3| (PREDICTED: vignain-like [Vitis vinifera])

HSP 1 Score: 451.1 bits (1159), Expect = 1.8e-123
Identity = 218/346 (63.01%), Postives = 270/346 (78.03%), Query Frame = 1

Query: 1   MPISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY 60
           M + K +LV   +VLV GLA+SF+FDE++LA++ SLW LYERW  +H +SR+L+EK+KR+
Sbjct: 1   MKMEKVILVALSLVLVFGLAESFDFDEKDLASEESLWDLYERWRSYHTVSRDLEEKNKRF 60

Query: 61  NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASG 120
           NVFKEN  HV  VNQM+KPYKLKLNKFADM+N+EF + Y  S + HYR L G RR G  G
Sbjct: 61  NVFKENTKHVHKVNQMDKPYKLKLNKFADMTNHEFRSSYGGSKVKHYRMLRGDRR-GTGG 120

Query: 121 FMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 180
           FM+EK T LP  +DWR++GAV  IK QG+CGSCWAFS V  VEGINQIKT +LLSLSEQ+
Sbjct: 121 FMHEKTTYLPPSVDWRKKGAVTGIKDQGKCGSCWAFSTVVGVEGINQIKTKELLSLSEQQ 180

Query: 181 LLDCN-TRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGF 240
           L+DC+ + + GC GG ME+A+ FI++NGGI +ENNYPY+     C   +M +P+VTIDG 
Sbjct: 181 LIDCDRSDDHGCNGGLMESAFEFIKKNGGITTENNYPYKAKDERCDMLKMNAPVVTIDGH 240

Query: 241 ESVPEN-ENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTD 300
           ESVP N E ALM+AVA+QPVSV+I+A G D QFY +GVFDG CGTEL+HGV ++GYGTT 
Sbjct: 241 ESVPVNDERALMKAVAHQPVSVAIDAGGSDLQFYSEGVFDGECGTELDHGVAIVGYGTTL 300

Query: 301 GGTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLK 345
            GT YW V+NSWG  WGE GYIRM RG++  EG CGIAMEASYP+K
Sbjct: 301 DGTKYWIVKNSWGAEWGEKGYIRMARGIQAAEGQCGIAMEASYPVK 345

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CYSEP_RICCO1.3e-12160.76Vignain OS=Ricinus communis GN=CYSEP PE=1 SV=1[more]
CYSEP_VIGMU4.6e-11959.25Vignain OS=Vigna mungo PE=1 SV=1[more]
CYSEP_PHAVU2.5e-11758.96Vignain OS=Phaseolus vulgaris PE=2 SV=2[more]
CEP3_ARATH4.2e-11258.19KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana GN=CEP3 PE=2 SV=... [more]
CEP2_ARATH4.6e-11156.65KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana GN=CEP2 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0LMU4_CUCSA2.5e-16481.50Uncharacterized protein OS=Cucumis sativus GN=Csa_2G349680 PE=3 SV=1[more]
W9RKI3_9ROSA1.5e-12462.72Uncharacterized protein OS=Morus notabilis GN=L484_027645 PE=3 SV=1[more]
D7SME9_VITVI1.3e-12363.01Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0137g00330 PE=3 SV=... [more]
M5XQB2_PRUPE2.8e-12364.43Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa025615mg PE=3 SV=1[more]
B9RMS9_RICCO3.1e-12260.29Cysteine protease, putative OS=Ricinus communis GN=RCOM_1083340 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G48350.12.3e-11358.19 Cysteine proteinases superfamily protein[more]
AT3G48340.12.6e-11256.65 Cysteine proteinases superfamily protein[more]
AT5G50260.11.7e-11155.23 Cysteine proteinases superfamily protein[more]
AT5G43060.14.2e-8649.69 Granulin repeat cysteine protease family protein[more]
AT4G35350.16.6e-8448.37 xylem cysteine peptidase 1[more]
Match NameE-valueIdentityDescription
gi|659087323|ref|XP_008444390.1|4.5e-16782.37PREDICTED: vignain-like [Cucumis melo][more]
gi|449450419|ref|XP_004142960.1|3.6e-16481.50PREDICTED: vignain-like [Cucumis sativus][more]
gi|703113247|ref|XP_010100336.1|2.1e-12462.72hypothetical protein L484_027645 [Morus notabilis][more]
gi|1009153182|ref|XP_015894499.1|2.1e-12462.72PREDICTED: vignain-like [Ziziphus jujuba][more]
gi|731372760|ref|XP_002285397.3|1.8e-12363.01PREDICTED: vignain-like [Vitis vinifera][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR000169Pept_cys_AS
IPR000668Peptidase_C1A_C
IPR013128Peptidase_C1A
IPR013201Prot_inhib_I29
IPR025660Pept_his_AS
IPR025661Pept_asp_AS
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Molecular Function
TermDefinition
GO:0008234cysteine-type peptidase activity
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0008234 cysteine-type peptidase activity
molecular_function GO:0032440 2-alkenal reductase [NAD(P)] activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmaCh12G000420CmaCh12G000420gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmaCh12G000420.1CmaCh12G000420.1-proteinpolypeptide


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh12G000420.1.exon.1CmaCh12G000420.1.exon.1exon
CmaCh12G000420.1.exon.2CmaCh12G000420.1.exon.2exon
CmaCh12G000420.1.exon.3CmaCh12G000420.1.exon.3exon
CmaCh12G000420.1.exon.4CmaCh12G000420.1.exon.4exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh12G000420.1.CDS.1CmaCh12G000420.1.CDS.1CDS
CmaCh12G000420.1.CDS.2CmaCh12G000420.1.CDS.2CDS
CmaCh12G000420.1.CDS.3CmaCh12G000420.1.CDS.3CDS
CmaCh12G000420.1.CDS.4CmaCh12G000420.1.CDS.4CDS


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000169Cysteine peptidase, cysteine active sitePROSITEPS00139THIOL_PROTEASE_CYScoord: 147..158
scor
IPR000668Peptidase C1A, papain C-terminalPRINTSPR00705PAPAINcoord: 147..162
score: 4.3E-10coord: 303..309
score: 4.3E-10coord: 287..297
score: 4.3
IPR000668Peptidase C1A, papain C-terminalPFAMPF00112Peptidase_C1coord: 129..342
score: 5.7
IPR000668Peptidase C1A, papain C-terminalSMARTSM00645pept_c1coord: 129..343
score: 2.0E
IPR013128Peptidase C1APANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 13..344
score: 7.9E
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 40..95
score: 8.9
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 40..95
score: 1.8
IPR025660Cysteine peptidase, histidine active sitePROSITEPS00639THIOL_PROTEASE_HIScoord: 285..295
scor
IPR025661Cysteine peptidase, asparagine active sitePROSITEPS00640THIOL_PROTEASE_ASNcoord: 303..322
scor
NoneNo IPR availableGENE3DG3DSA:3.90.70.10coord: 17..343
score: 1.8E
NoneNo IPR availablePANTHERPTHR12411:SF346KDEL-TAILED CYSTEINE ENDOPEPTIDASE CEP1-RELATEDcoord: 13..344
score: 7.9E
NoneNo IPR availableunknownSSF54001Cysteine proteinasescoord: 32..343
score: 3.32