CmaCh10G000820.1 (mRNA) Cucurbita maxima (Rimu)

NameCmaCh10G000820.1
TypemRNA
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionProtein-L-isoaspartate O-methyltransferase
LocationCma_Chr10 : 342983 .. 346462 (+)
Sequence length1165
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGGAGAAAATGAATTTAACTCAAATATCCCCGTCACTCCGCTATTGTGCGCCTTCTATTGGACTGTACTTCAACACCCCCTCATCCCTCCGCCGCCACCGCCGTCTCCCTCTCCGTCTTCCCACCGCCCTACCACCGACCGCTTCTTCTGTTCCGAAAACCACCACCACTATCATCCCCTTCTTACCTCGATTTCTAAACCCTCATTTCGTCACGGGAAATTGTCTTCTTTCCAGGATGGAGGTACTCTCTCCCTCTCTCTCTATCTACCGCTAATTCCCACCGATTTTCGTTATTTGAAATTGGAGATACTTGGATTTCCCCAAAATGGATGAATTGCCCCCCCATCCATTTCTGACGCTGAAGGTATATCGTGTTTGTTTGTTTGTATGTGCATCCACGACCCAACTTTTGATATTTTTGTATACGATTTTAGAAGGAAGAAGCTGTGTAAAAATTTGTTCGTCTTTCTCTCTTATCTGGATTTTGGATAATTCGAAATTGTTCCATAGAGAAGCATCGCTTTGAAACTTCTTGATACATTCTTGAATCTTCATTTTAATCATTCACTGTATTTTTTTTTTTTTTCAATACATGTAGTTTCAGGGGATTTTAATTCGAGTGGTATGTGTAGTGTATTCTATTCTGGTATATTTTCCTTGGGATGTGCAATCTTTTGATAATGATATTCGACGGCATGCATGTCCGAGTGTATTGGACGGACAGGTTTCGTCCCTAAACGGCCCGGCTTCTGAGGCTGTGGAGATTGATAAGGTAATTGCATTAACGATAATGAGCCAAGGGTCGCATTGAAGATGAATAGCTTTATGAATTAATGAGGTAGACTTAGGAATTCCTTTGTGAATTGTTTATTATTGTTGTGTTTCTATTAAGGGGAAGTATTTTTCGTTTTCAATTCTTTATAAGGGGTTGAATGTTTGATCGTAGTATCACATTGCAGAGATTCTGGAGTGGAAGTGGCATAGGTACAAACAAAGAAATGGTGGAGAATCTCCAGAGCTATGGAGTGGTTAGGTCAAAGAAGGTTTCTGAAGTAATGGAGAGTGTAGATAGGGCCTTCTTTGTACCGGATGGTGTTCCTCCTTATGTCGATACTCCCCTGCCGATAGGTTACAATGCCACTATATCTGCACCTCACATGCATGCTACTTGCCTTCAGTTGTTGGAGAAACACTTGCAGCCTGGAATGCGAGCTCTGGACGTTGGCTCAGGTTATCCATCTTACTGACATTTTCTACGTACTGAAAATTGCATTCGTGGTTTTATCGTAACTGTTGAGTTCCTATGGATGCCCAACTATGTTTGAGTTCTAAGTAACTTATAATCATAAATATATATTGTGAGATCCCACATTGTTTGGGGAGTAGAATGAAACATTCTTTATAAAGGTGTGGAAACTTCAACCTAATAGACACATTTTAAAAACTTTGAGGGAAAGCTAAAGAGGACAATATGAGTTTGGACATTGTTGACGATTGTTGGGAGAGAGTTCCACATAGGCTAGTTTAGGAAATGATCATGGGTTTATAATTAAGGAATACATCCCCATTGGTATGAGGCCTTTTGGGGAAGCCCAAAGCAAAGACATGAGAGCTTATGATCAAAGTCGACAATATCATACTATTGTGGAGAGTCATGATTTCTAACAGACATTTCCTCAAGGATTTGATGTATTCTGTAATGACCCAGATCCATTGCTAGAAGATATTGTCCTCTTTGGGCTTTCCCTTTCGGGCTTCCCCTCAAGGCTTTAAAACGCGTCTGCTAGGGGAAGGTTTCCACACCCTTATATATGGTGATTTGTTCTCCTCCCCAACTAATGTGGGACATCATAATCCACCCCCCTTCGAGGCCCAGCGTCCTTGCTGGCACTCTTTTCCTTCCTCCAATCGATGTGGGACCGCCCCCAAATCCATCCCCTTTGGGGCCGAGCGTCCTTACTGGCACACCATCTCGTGTTTACTCCCCTTCGGGGAACAGCGAGAAGGCTGGCAATCGTCCGGTACCTGGCTCTGATACCATTTGTAACGACCCAAGATCCACCGCTTGCAGATATTGTCTCTTTGGGCTTTTTCTTTCGGACTTCCCCTCAAGACTTTAAAACGCGTCTGCTAGGAGAAGGTTTTCACACCCTTATATATGGTGATTTGTTCTCCTCCCCAACCAATGTGAGATATCACATATTCTCCATCTTTACTTTTACTTGCACTTCTTGAGTGCTCCCCACCAAAAGAAAAGAAAAACATCTAAATTGTAGAAGAATTATTGTGGTTTGTCCTACAAGGGTTCAGTGTTCTCTTGACACACACTACCCAATTGAAAATGCTATCTGGTTAGTGAAGGTTGTGGGTATTCATTCTTCAAGATTCGATTTTTTCGACTACTTGATTTCCAGATAGGGCTAAAATGCTAGCTCAAAAGGGCAAACAGACAAGGTTCCTCCGGCTGTTTATTACTCTAAGAAGATACGATTTTATTACCCTTCATGTTTGAAATTGGATTTTCAACTCCAAAATATCAATTCCTCGACCCCTACCCGTTCCTTTATGTTTTAAATTGGTCACCTTGGCAGGAACGGGATACTTGACTGCATGCTTTGCATTAATGGTTGGACCCGAAGGTCGTGCCGTTGGTGTGGAACATATTCCTGAGCTGGTTGCTTCCTCAATCAAGAATATTGAAAAAAGTGCTGCTGCTCCTTTACTGAAAAAAGGCTCCCTCTCTGTACATGTTGGTGGTACGTTCGTCTTCTTTTCCAGTTCCTAGAGCCATAATTGTTCTTTTAATCTAGAATAGGAACTCTTTTCCTTAGTTTGGTTGATCCGTCAAGTGATATTCTTGCACCCATTTTATTTTTGAACTGGAATGATATATTCTTGAGAGTAATGGTAGTTTACTTTCGAGTGAAACACTTCGAAAACAAGACACTTTCACGACAAGACTTCGGTTAGTTATTGTTATTGGATGTTGTAGATGGCAGGCAGGGTTGGGCAGAGTGTGCACCGTACGACGCCATTCATGTTGGAGCAGCTGCTGCTGAAATTCCTCCGGCTCTCATTGACCAGTTAAAACCAGGCGGGAGAATGGTGATTCCAGTTGGGAACATATTCCAGGATTTAAAGGTTGTGGACAAAGATACCGATGGCTCTATTAACATCCACGACGAGACTTCTGTTCGTTATGTGCCCCTTACCAGTCGAGAAGCTCAGTTAGGTGACAACTGATCTGGTTTGCGCATAGCTTTTATCAAGAATGCTGTATAGGAACTTTTTGAAACCGTTTGTTGAACTATATCATCCTTGTAAATTTAATCCTCAATGTGGATATTTGGTCCAAAATAAGTGTGTTTGACACATTTATCATTACGAAATTTCAACAACCCTTTGGCCATGTTCTCGAGGGGGTTCATGTTTCAATGATGGATCGTTTATTCCCGGATGATTTGTTCGTCTCCTTCAG

mRNA sequence

AAGGAGAAAATGAATTTAACTCAAATATCCCCGTCACTCCGCTATTGTGCGCCTTCTATTGGACTGTACTTCAACACCCCCTCATCCCTCCGCCGCCACCGCCGTCTCCCTCTCCGTCTTCCCACCGCCCTACCACCGACCGCTTCTTCTGTTCCGAAAACCACCACCACTATCATCCCCTTCTTACCTCGATTTCTAAACCCTCATTTCGTCACGGGAAATTGTCTTCTTTCCAGGATGGAGAGATTCTGGAGTGGAAGTGGCATAGGTACAAACAAAGAAATGGTGGAGAATCTCCAGAGCTATGGAGTGGTTAGGTCAAAGAAGGTTTCTGAAGTAATGGAGAGTGTAGATAGGGCCTTCTTTGTACCGGATGGTGTTCCTCCTTATGTCGATACTCCCCTGCCGATAGGTTACAATGCCACTATATCTGCACCTCACATGCATGCTACTTGCCTTCAGTTGTTGGAGAAACACTTGCAGCCTGGAATGCGAGCTCTGGACGTTGGCTCAGGAACGGGATACTTGACTGCATGCTTTGCATTAATGGTTGGACCCGAAGGTCGTGCCGTTGGTGTGGAACATATTCCTGAGCTGGTTGCTTCCTCAATCAAGAATATTGAAAAAAGTGCTGCTGCTCCTTTACTGAAAAAAGGCTCCCTCTCTGTACATGTTGGTGATGGCAGGCAGGGTTGGGCAGAGTGTGCACCGTACGACGCCATTCATGTTGGAGCAGCTGCTGCTGAAATTCCTCCGGCTCTCATTGACCAGTTAAAACCAGGCGGGAGAATGGTGATTCCAGTTGGGAACATATTCCAGGATTTAAAGGTTGTGGACAAAGATACCGATGGCTCTATTAACATCCACGACGAGACTTCTGTTCGTTATGTGCCCCTTACCAGTCGAGAAGCTCAGTTAGGTGACAACTGATCTGGTTTGCGCATAGCTTTTATCAAGAATGCTGTATAGGAACTTTTTGAAACCGTTTGTTGAACTATATCATCCTTGTAAATTTAATCCTCAATGTGGATATTTGGTCCAAAATAAGTGTGTTTGACACATTTATCATTACGAAATTTCAACAACCCTTTGGCCATGTTCTCGAGGGGGTTCATGTTTCAATGATGGATCGTTTATTCCCGGATGATTTGTTCGTCTCCTTCAG

Coding sequence (CDS)

ATGAATTTAACTCAAATATCCCCGTCACTCCGCTATTGTGCGCCTTCTATTGGACTGTACTTCAACACCCCCTCATCCCTCCGCCGCCACCGCCGTCTCCCTCTCCGTCTTCCCACCGCCCTACCACCGACCGCTTCTTCTGTTCCGAAAACCACCACCACTATCATCCCCTTCTTACCTCGATTTCTAAACCCTCATTTCGTCACGGGAAATTGTCTTCTTTCCAGGATGGAGAGATTCTGGAGTGGAAGTGGCATAGGTACAAACAAAGAAATGGTGGAGAATCTCCAGAGCTATGGAGTGGTTAGGTCAAAGAAGGTTTCTGAAGTAATGGAGAGTGTAGATAGGGCCTTCTTTGTACCGGATGGTGTTCCTCCTTATGTCGATACTCCCCTGCCGATAGGTTACAATGCCACTATATCTGCACCTCACATGCATGCTACTTGCCTTCAGTTGTTGGAGAAACACTTGCAGCCTGGAATGCGAGCTCTGGACGTTGGCTCAGGAACGGGATACTTGACTGCATGCTTTGCATTAATGGTTGGACCCGAAGGTCGTGCCGTTGGTGTGGAACATATTCCTGAGCTGGTTGCTTCCTCAATCAAGAATATTGAAAAAAGTGCTGCTGCTCCTTTACTGAAAAAAGGCTCCCTCTCTGTACATGTTGGTGATGGCAGGCAGGGTTGGGCAGAGTGTGCACCGTACGACGCCATTCATGTTGGAGCAGCTGCTGCTGAAATTCCTCCGGCTCTCATTGACCAGTTAAAACCAGGCGGGAGAATGGTGATTCCAGTTGGGAACATATTCCAGGATTTAAAGGTTGTGGACAAAGATACCGATGGCTCTATTAACATCCACGACGAGACTTCTGTTCGTTATGTGCCCCTTACCAGTCGAGAAGCTCAGTTAGGTGACAACTGA

Protein sequence

MNLTQISPSLRYCAPSIGLYFNTPSSLRRHRRLPLRLPTALPPTASSVPKTTTTIIPFLPRFLNPHFVTGNCLLSRMERFWSGSGIGTNKEMVENLQSYGVVRSKKVSEVMESVDRAFFVPDGVPPYVDTPLPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMVGPEGRAVGVEHIPELVASSIKNIEKSAAAPLLKKGSLSVHVGDGRQGWAECAPYDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQDLKVVDKDTDGSINIHDETSVRYVPLTSREAQLGDN
BLAST of CmaCh10G000820.1 vs. Swiss-Prot
Match: PIMT1_ARATH (Protein-L-isoaspartate O-methyltransferase 1 OS=Arabidopsis thaliana GN=PIMT1 PE=1 SV=3)

HSP 1 Score: 363.6 bits (932), Expect = 2.1e-99
Identity = 177/227 (77.97%), Postives = 201/227 (88.55%), Query Frame = 1

Query: 77  MERFWSGSGIGTNKEMVENLQSYGVVRSKKVSEVMESVDRAFFVPDGVPPYVDTPLPIGY 136
           M++FWS S I  NK MVENLQ++G+V S +V++ ME+VDR  FV D    YVD+P+ IGY
Sbjct: 1   MKQFWSPSSINKNKAMVENLQNHGIVTSDEVAKAMEAVDRGVFVTDRSSAYVDSPMSIGY 60

Query: 137 NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMVGPEGRAVGVEHIPEL 196
           N TISAPHMHA CLQLLEKHL+PGMR LDVGSGTGYLTACFA+MVG EGRA+GVEHIPEL
Sbjct: 61  NVTISAPHMHAMCLQLLEKHLKPGMRVLDVGSGTGYLTACFAVMVGTEGRAIGVEHIPEL 120

Query: 197 VASSIKNIEKSAAAPLLKKGSLSVHVGDGRQGWAECAPYDAIHVGAAAAEIPPALIDQLK 256
           VASS+KNIE SAA+P LK+GSL+VHVGDGRQGWAE APYDAIHVGAAA EIP ALIDQLK
Sbjct: 121 VASSVKNIEASAASPFLKEGSLAVHVGDGRQGWAEFAPYDAIHVGAAAPEIPEALIDQLK 180

Query: 257 PGGRMVIPVGNIFQDLKVVDKDTDGSINIHDETSVRYVPLTSREAQL 304
           PGGR+VIPVGNIFQDL+VVDK++DGS++I DETSVRYVPLTSREAQL
Sbjct: 181 PGGRLVIPVGNIFQDLQVVDKNSDGSVSIKDETSVRYVPLTSREAQL 227

BLAST of CmaCh10G000820.1 vs. Swiss-Prot
Match: PIMT2_ARATH (Protein-L-isoaspartate O-methyltransferase 2 OS=Arabidopsis thaliana GN=PIMT2 PE=1 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 3.1e-95
Identity = 172/232 (74.14%), Postives = 193/232 (83.19%), Query Frame = 1

Query: 73  LLSRMERFWSGSGIGTNKEMVENLQSYGVVRSKKVSEVMESVDRAFFVPDGVPPYVDTPL 132
           L   ME+F SG+G    + MVENL+ YGV+ SK+V++VME++DR  FVP G   YVDTP+
Sbjct: 76  LFFHMEQFQSGTGSSGKRGMVENLKRYGVISSKRVAQVMEALDRGLFVPVGSSAYVDTPV 135

Query: 133 PIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMVGPEGRAVGVEH 192
           PIGYNATISAPHMHATCLQLLE  L PGMRALDVGSGTGYLT CFALMVG EGR VGV+H
Sbjct: 136 PIGYNATISAPHMHATCLQLLEDKLHPGMRALDVGSGTGYLTGCFALMVGAEGRVVGVDH 195

Query: 193 IPELVASSIKNIEKSAAAPLLKKGSLSVHVGDGRQGWAECAPYDAIHVGAAAAEIPPALI 252
           IPELV  SIKNIEKS AA  LKKGSLS+HVGDGR+GW E APYDAIHVGAAA+EIP  L+
Sbjct: 196 IPELVDMSIKNIEKSVAASFLKKGSLSLHVGDGRKGWQEFAPYDAIHVGAAASEIPQPLL 255

Query: 253 DQLKPGGRMVIPVGNIFQDLKVVDKDTDGSINIHDETSVRYVPLTSREAQLG 305
           DQLKPGGRMVIP+G  FQ+LKV+DK+ DGSI +H ETSVRYVPLTSR  QLG
Sbjct: 256 DQLKPGGRMVIPLGTYFQELKVIDKNEDGSIKVHTETSVRYVPLTSRVEQLG 307

BLAST of CmaCh10G000820.1 vs. Swiss-Prot
Match: PIMT_WHEAT (Protein-L-isoaspartate O-methyltransferase OS=Triticum aestivum GN=PCM PE=1 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 4.7e-91
Identity = 158/230 (68.70%), Postives = 191/230 (83.04%), Query Frame = 1

Query: 77  MERFWSGSGIGTNKEMVENLQSYGVVRSKKVSEVMESVDRAFFVPDGVPPYVDTPLPIGY 136
           M +FW+   +  N  +VE L+ YGVVR+ KV+EVME++DRA FVP+G  PY D+P+PIGY
Sbjct: 1   MAQFWAEGSLEKNNALVEYLKQYGVVRTDKVAEVMETIDRALFVPEGFTPYTDSPMPIGY 60

Query: 137 NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMVGPEGRAVGVEHIPEL 196
           NATISAPHMHATCL+LL+ +LQPGM ALDVGSG+GYLTACFA+MVGPEGRAVG+EHIPEL
Sbjct: 61  NATISAPHMHATCLELLKDYLQPGMHALDVGSGSGYLTACFAMMVGPEGRAVGIEHIPEL 120

Query: 197 VASSIKNIEKSAAAPLLKKGSLSVHVGDGRQGWAECAPYDAIHVGAAAAEIPPALIDQLK 256
           V +S +N+E+SAAA L+K GSLS HV DGR GW + APYDAIHVGAAA EIP  L++QLK
Sbjct: 121 VVASTENVERSAAAALMKDGSLSFHVSDGRLGWPDAAPYDAIHVGAAAPEIPRPLLEQLK 180

Query: 257 PGGRMVIPVGNIFQDLKVVDKDTDGSINIHDETSVRYVPLTSREAQLGDN 307
           PGGRMVIPVG   QDL+V+DK  DGS ++ ++ SVRYVPLTSR AQL D+
Sbjct: 181 PGGRMVIPVGTYSQDLQVIDKSADGSTSVRNDASVRYVPLTSRSAQLQDS 230

BLAST of CmaCh10G000820.1 vs. Swiss-Prot
Match: PIMT_CHICK (Protein-L-isoaspartate(D-aspartate) O-methyltransferase OS=Gallus gallus GN=PCMT1 PE=2 SV=3)

HSP 1 Score: 220.3 bits (560), Expect = 2.9e-56
Identity = 117/221 (52.94%), Postives = 151/221 (68.33%), Query Frame = 1

Query: 84  SGIGTNKEMVENLQSYGVVRSKKVSEVMESVDRAFFVPDGVPPYVDTPLPIGYNATISAP 143
           SG  ++ E++ NL+  G+++S KV EVM + DR  +      PY+D+P  IG+ ATISAP
Sbjct: 5   SGGASHSELIHNLRKNGIIKSDKVFEVMLATDRCHYAKYN--PYMDSPQSIGFQATISAP 64

Query: 144 HMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMVGPEGRAVGVEHIPELVASSIKN 203
           HMHA  L+LL   L  G +ALDVGSG+G LTACF+ MVGP+G+ VG++HI ELV  SI N
Sbjct: 65  HMHAYALELLSDQLHEGAKALDVGSGSGILTACFSRMVGPKGQVVGIDHIKELVDDSINN 124

Query: 204 IEKSAAAPLLKKGSLSVHVGDGRQGWAECAPYDAIHVGAAAAEIPPALIDQLKPGGRMVI 263
           ++K     LL  G + + VGDGR G+AE APYDAIHVGAAA  +P ALIDQLKPGGR+++
Sbjct: 125 VKKDDPT-LLSSGRVKLIVGDGRMGYAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLIL 184

Query: 264 PVGNI--FQDLKVVDKDTDGSINIHDETSVRYVPLTSREAQ 303
           PVG     Q L+  DK  DGS+ +     V YVPLT +E Q
Sbjct: 185 PVGPAGGNQMLEQYDKLEDGSVKMKPLMGVIYVPLTDKEKQ 222

BLAST of CmaCh10G000820.1 vs. Swiss-Prot
Match: PIMT_PIG (Protein-L-isoaspartate(D-aspartate) O-methyltransferase OS=Sus scrofa GN=PCMT1 PE=1 SV=3)

HSP 1 Score: 218.4 bits (555), Expect = 1.1e-55
Identity = 116/221 (52.49%), Postives = 150/221 (67.87%), Query Frame = 1

Query: 84  SGIGTNKEMVENLQSYGVVRSKKVSEVMESVDRAFFVPDGVPPYVDTPLPIGYNATISAP 143
           SG  ++ E++ NL+  G++++ KV EVM + DR+ +      PY+D+P  IG+ ATISAP
Sbjct: 5   SGGASHSELIHNLRKNGIIKTDKVFEVMLATDRSHYAKCN--PYMDSPQSIGFQATISAP 64

Query: 144 HMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMVGPEGRAVGVEHIPELVASSIKN 203
           HMHA  L+LL   L  G +ALDVGSG+G LTACFA MVGP G+ +G++HI ELV  SI N
Sbjct: 65  HMHAYALELLFDQLHEGAKALDVGSGSGILTACFARMVGPSGKVIGIDHIKELVDDSINN 124

Query: 204 IEKSAAAPLLKKGSLSVHVGDGRQGWAECAPYDAIHVGAAAAEIPPALIDQLKPGGRMVI 263
           + K     LL  G + + VGDGR G+AE APYDAIHVGAAA  +P ALIDQLKPGGR+++
Sbjct: 125 VRKDDPM-LLSSGRVQLVVGDGRMGYAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLIL 184

Query: 264 PVGNI--FQDLKVVDKDTDGSINIHDETSVRYVPLTSREAQ 303
           PVG     Q L+  DK  DGS+ +     V YVPLT +E Q
Sbjct: 185 PVGPAGGNQMLEQYDKLQDGSVKMKPLMGVIYVPLTDKEKQ 222

BLAST of CmaCh10G000820.1 vs. TrEMBL
Match: A0A0A0LRC6_CUCSA (Protein-L-isoaspartate O-methyltransferase OS=Cucumis sativus GN=Csa_1G002880 PE=3 SV=1)

HSP 1 Score: 533.9 bits (1374), Expect = 1.3e-148
Identity = 267/313 (85.30%), Postives = 289/313 (92.33%), Query Frame = 1

Query: 1   MNLTQISPSL------RYCAPSIGLYFNTPSSLRRHRRLPLRLPTALPPTASSVPKTT-T 60
           M++TQIS S       RYCAPS+GL+FN   SL R RR P  L TALPPT SSVPKTT T
Sbjct: 1   MSITQISLSFPLAYASRYCAPSLGLFFNL--SLHRRRRRPRLLSTALPPTTSSVPKTTVT 60

Query: 61  TIIPFLPRFLNPHFVTGNCLLSRMERFWSGSGIGTNKEMVENLQSYGVVRSKKVSEVMES 120
           TI+ FLPRFL+PHF+TGNCLLSRMERFWSGSGIGTNK+MVE LQ+YGVVRSK+VSEVMES
Sbjct: 61  TIVSFLPRFLDPHFLTGNCLLSRMERFWSGSGIGTNKDMVEKLQTYGVVRSKRVSEVMES 120

Query: 121 VDRAFFVPDGVPPYVDTPLPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYL 180
           +DRAFFVPD VPPYVDTP+PIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYL
Sbjct: 121 IDRAFFVPDDVPPYVDTPVPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYL 180

Query: 181 TACFALMVGPEGRAVGVEHIPELVASSIKNIEKSAAAPLLKKGSLSVHVGDGRQGWAECA 240
           TACFALMVGPEGR VGVEHIPELVASS++NI+KSAAAPLLK+GSLS+HVGDGRQGWAECA
Sbjct: 181 TACFALMVGPEGRVVGVEHIPELVASSMENIKKSAAAPLLKEGSLSLHVGDGRQGWAECA 240

Query: 241 PYDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQDLKVVDKDTDGSINIHDETSVRY 300
           PYDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGN+FQDLKVVDKD+DGS++IHDETSVRY
Sbjct: 241 PYDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGNVFQDLKVVDKDSDGSVSIHDETSVRY 300

Query: 301 VPLTSREAQLGDN 307
           VPLTSREAQL D+
Sbjct: 301 VPLTSREAQLRDD 311

BLAST of CmaCh10G000820.1 vs. TrEMBL
Match: A0A068V3L9_COFCA (Protein-L-isoaspartate O-methyltransferase OS=Coffea canephora GN=GSCOC_T00042669001 PE=3 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 2.0e-112
Identity = 214/301 (71.10%), Postives = 235/301 (78.07%), Query Frame = 1

Query: 6   ISPSLRYCAP---SIGLYFNTPSSLRRHRRLPLRLPTALPPTASSVPKTTTTIIPFLPRF 65
           I+   RY AP   S   +     SL  H R   RLP   P + + +   TT       R 
Sbjct: 20  IACGCRYRAPLKHSFLAFTRRFHSLHPHHRYCRRLP---PHSLAILAANTTAYSSRSRRL 79

Query: 66  LNPHFVTGNCLLSRMERFWSGSGIGTNKEMVENLQSYGVVRSKKVSEVMESVDRAFFVPD 125
              HF TGN L SRMERFW+G GI  N+ MVE+LQSYGVVRSKKV+EVM ++DRA FVPD
Sbjct: 80  PKTHFFTGNSLFSRMERFWTGGGIDKNRAMVEHLQSYGVVRSKKVAEVMGTIDRALFVPD 139

Query: 126 GVPPYVDTPLPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMVG 185
           G PPYVDTP+ IGYNATISAPHMHA CL+LLE HLQPGM ALD+GSGTGYLTACFA+MVG
Sbjct: 140 GTPPYVDTPMQIGYNATISAPHMHAMCLELLENHLQPGMHALDIGSGTGYLTACFAIMVG 199

Query: 186 PEGRAVGVEHIPELVASSIKNIEKSAAAPLLKKGSLSVHVGDGRQGWAECAPYDAIHVGA 245
           P+G AVGVEHIPEL A SIKNI+KSAAAPLLK+GSLS+H GDGR GW E APYDAIHVGA
Sbjct: 200 PQGHAVGVEHIPELAAESIKNIQKSAAAPLLKEGSLSIHAGDGRLGWPEHAPYDAIHVGA 259

Query: 246 AAAEIPPALIDQLKPGGRMVIPVGNIFQDLKVVDKDTDGSINIHDETSVRYVPLTSREAQ 304
           AA EIP ALIDQLKPGGRMVIPVGNIFQDLKVVDKD DGSI++H ETSVRYVPLTSREAQ
Sbjct: 260 AAPEIPQALIDQLKPGGRMVIPVGNIFQDLKVVDKDMDGSISVHSETSVRYVPLTSREAQ 317

BLAST of CmaCh10G000820.1 vs. TrEMBL
Match: A0A061FZ18_THECC (Protein-L-isoaspartate O-methyltransferase OS=Theobroma cacao GN=TCM_014514 PE=3 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 4.8e-111
Identity = 216/306 (70.59%), Postives = 243/306 (79.41%), Query Frame = 1

Query: 1   MNL-TQISPSLRYCAPSIG--LYFNTPSSLRRHRRLPLRLPTALPPTASSVPKTTTTIIP 60
           MNL T I+   RYC P I   L + T   L  H  L L L   L   + +    ++    
Sbjct: 2   MNLSTVIAYGCRYCTPPIKQLLNYTTLLHLPHHHHLHLHLHLHLQRRSPASIFYSSFNNL 61

Query: 61  FLPRFLNPHFVTGNCLLSRMERFWSGSGIGTNKEMVENLQSYGVVRSKKVSEVMESVDRA 120
            LP F     +TGN L  RMERFWSG+G+  NK+MVE+LQ YGV+ SKKV+EVME+VDRA
Sbjct: 62  KLPNF--SLLLTGNSLFFRMERFWSGNGVNKNKQMVEHLQHYGVISSKKVAEVMETVDRA 121

Query: 121 FFVPDGVPPYVDTPLPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACF 180
           FFVPDG PPYVD+P+ IGYNATISAPHMHATCLQLLE++LQPGM ALDVGSGTGYLTACF
Sbjct: 122 FFVPDGTPPYVDSPMAIGYNATISAPHMHATCLQLLEENLQPGMHALDVGSGTGYLTACF 181

Query: 181 ALMVGPEGRAVGVEHIPELVASSIKNIEKSAAAPLLKKGSLSVHVGDGRQGWAECAPYDA 240
           A+MVG +GRAVGVEHIPELVASSI+NI+K AAAPLLK+GSLSVH GDGRQGW ECAPYDA
Sbjct: 182 AIMVGRQGRAVGVEHIPELVASSIENIKKCAAAPLLKEGSLSVHTGDGRQGWPECAPYDA 241

Query: 241 IHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQDLKVVDKDTDGSINIHDETSVRYVPLT 300
           IHVGAAA EIP  L+DQLKPGGRMVIPVGNIFQDLKVVDK+ DGSI+I  ETSVRYVPLT
Sbjct: 242 IHVGAAAPEIPQPLLDQLKPGGRMVIPVGNIFQDLKVVDKNLDGSISIRSETSVRYVPLT 301

Query: 301 SREAQL 304
           SR+AQL
Sbjct: 302 SRDAQL 305

BLAST of CmaCh10G000820.1 vs. TrEMBL
Match: M5WF21_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa006554mg PE=4 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 6.3e-111
Identity = 214/309 (69.26%), Postives = 243/309 (78.64%), Query Frame = 1

Query: 1   MNLTQISPSLRYCAPSIG--LYFNT----PSSLRRHRRLPLRLPTALPPTASSVPKTTTT 60
           + L +++   RY AP++   L F T    P S  RHR  P  L T LP          TT
Sbjct: 106 VGLDRLAYGSRYPAPTVQHLLTFTTLSFFPHSHHRHRHRPRTL-TLLP----------TT 165

Query: 61  IIPFLPRFLNPHFVTGNCLLSRMERFWSGSGIGTNKEMVENLQSYGVVRSKKVSEVMESV 120
            + F P   NP+F+TGN L   ME+FWS S +  NK MVENLQSYGV+ SKKV EVM ++
Sbjct: 166 SLSFCPLLQNPNFLTGNSLFRGMEQFWSHSSVKKNKAMVENLQSYGVISSKKVVEVMGTI 225

Query: 121 DRAFFVPDGVPPYVDTPLPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLT 180
           DRA FVPDG PPYVD+P+ IGYNATISAPHMHATCLQLLE++LQP MRALD+GSGTGYLT
Sbjct: 226 DRALFVPDGTPPYVDSPMAIGYNATISAPHMHATCLQLLEENLQPNMRALDIGSGTGYLT 285

Query: 181 ACFALMVGPEGRAVGVEHIPELVASSIKNIEKSAAAPLLKKGSLSVHVGDGRQGWAECAP 240
           ACFALMVGP+GRAVGVEHIPEL +SSI+NI+KSAAAPLLK+GSLSVHVGDGR GW E AP
Sbjct: 286 ACFALMVGPQGRAVGVEHIPELASSSIENIKKSAAAPLLKEGSLSVHVGDGRLGWPEFAP 345

Query: 241 YDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQDLKVVDKDTDGSINIHDETSVRYV 300
           YDAIHVGAAA EIP  LIDQLKPGGRMVIPVGNIFQDLKVVDK+ DGSI++  ETSVRYV
Sbjct: 346 YDAIHVGAAAPEIPQPLIDQLKPGGRMVIPVGNIFQDLKVVDKNLDGSISVRTETSVRYV 403

Query: 301 PLTSREAQL 304
           PLTS++AQL
Sbjct: 406 PLTSKDAQL 403

BLAST of CmaCh10G000820.1 vs. TrEMBL
Match: V4VKI5_9ROSI (Protein-L-isoaspartate O-methyltransferase OS=Citrus clementina GN=CICLE_v10021331mg PE=3 SV=1)

HSP 1 Score: 404.1 bits (1037), Expect = 1.6e-109
Identity = 199/264 (75.38%), Postives = 228/264 (86.36%), Query Frame = 1

Query: 46  SSVPKTTTTIIPFLPRFLNPH------FVTGNCLLSRMERFWSGSGIGTNKEMVENLQSY 105
           S +P  + +++     F  P+      F+TGNC   +MER+WSGSG+  NK MVE+LQ Y
Sbjct: 37  SYLPSPSPSLVSLSSSFHLPNPDNLSRFLTGNCPFFKMERYWSGSGVNKNKAMVEHLQHY 96

Query: 106 GVVRSKKVSEVMESVDRAFFVPDGVPPYVDTPLPIGYNATISAPHMHATCLQLLEKHLQP 165
           GV+ SKKVSEVME++DRA FVPDG PPYVD+P+ IGYNATISAPHMHATCLQLLE++L+P
Sbjct: 97  GVITSKKVSEVMETIDRACFVPDGTPPYVDSPMAIGYNATISAPHMHATCLQLLEENLKP 156

Query: 166 GMRALDVGSGTGYLTACFALMVGPEGRAVGVEHIPELVASSIKNIEKSAAAPLLKKGSLS 225
           GM ALD+GSGTGYLTACFALMVGP+GRAVGVEHIPELVASSI+NIEKSAAAPLLK+GSLS
Sbjct: 157 GMHALDIGSGTGYLTACFALMVGPQGRAVGVEHIPELVASSIQNIEKSAAAPLLKEGSLS 216

Query: 226 VHVGDGRQGWAECAPYDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQDLKVVDKDT 285
           VHVGDGR+GW E APYDAIHVGAAA EIP ALIDQLKPGGRMVIPVGNIFQDLKVVDK+ 
Sbjct: 217 VHVGDGRKGWPEFAPYDAIHVGAAAPEIPQALIDQLKPGGRMVIPVGNIFQDLKVVDKNQ 276

Query: 286 DGSINIHDETSVRYVPLTSREAQL 304
           DGS+++  ETSVRYVPLTSR+AQL
Sbjct: 277 DGSLSVWSETSVRYVPLTSRDAQL 300

BLAST of CmaCh10G000820.1 vs. TAIR10
Match: AT3G48330.1 (AT3G48330.1 protein-l-isoaspartate methyltransferase 1)

HSP 1 Score: 363.6 bits (932), Expect = 1.2e-100
Identity = 177/227 (77.97%), Postives = 201/227 (88.55%), Query Frame = 1

Query: 77  MERFWSGSGIGTNKEMVENLQSYGVVRSKKVSEVMESVDRAFFVPDGVPPYVDTPLPIGY 136
           M++FWS S I  NK MVENLQ++G+V S +V++ ME+VDR  FV D    YVD+P+ IGY
Sbjct: 1   MKQFWSPSSINKNKAMVENLQNHGIVTSDEVAKAMEAVDRGVFVTDRSSAYVDSPMSIGY 60

Query: 137 NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMVGPEGRAVGVEHIPEL 196
           N TISAPHMHA CLQLLEKHL+PGMR LDVGSGTGYLTACFA+MVG EGRA+GVEHIPEL
Sbjct: 61  NVTISAPHMHAMCLQLLEKHLKPGMRVLDVGSGTGYLTACFAVMVGTEGRAIGVEHIPEL 120

Query: 197 VASSIKNIEKSAAAPLLKKGSLSVHVGDGRQGWAECAPYDAIHVGAAAAEIPPALIDQLK 256
           VASS+KNIE SAA+P LK+GSL+VHVGDGRQGWAE APYDAIHVGAAA EIP ALIDQLK
Sbjct: 121 VASSVKNIEASAASPFLKEGSLAVHVGDGRQGWAEFAPYDAIHVGAAAPEIPEALIDQLK 180

Query: 257 PGGRMVIPVGNIFQDLKVVDKDTDGSINIHDETSVRYVPLTSREAQL 304
           PGGR+VIPVGNIFQDL+VVDK++DGS++I DETSVRYVPLTSREAQL
Sbjct: 181 PGGRLVIPVGNIFQDLQVVDKNSDGSVSIKDETSVRYVPLTSREAQL 227

BLAST of CmaCh10G000820.1 vs. TAIR10
Match: AT5G50240.1 (AT5G50240.1 protein-l-isoaspartate methyltransferase 2)

HSP 1 Score: 349.7 bits (896), Expect = 1.8e-96
Identity = 172/232 (74.14%), Postives = 193/232 (83.19%), Query Frame = 1

Query: 73  LLSRMERFWSGSGIGTNKEMVENLQSYGVVRSKKVSEVMESVDRAFFVPDGVPPYVDTPL 132
           L   ME+F SG+G    + MVENL+ YGV+ SK+V++VME++DR  FVP G   YVDTP+
Sbjct: 76  LFFHMEQFQSGTGSSGKRGMVENLKRYGVISSKRVAQVMEALDRGLFVPVGSSAYVDTPV 135

Query: 133 PIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMVGPEGRAVGVEH 192
           PIGYNATISAPHMHATCLQLLE  L PGMRALDVGSGTGYLT CFALMVG EGR VGV+H
Sbjct: 136 PIGYNATISAPHMHATCLQLLEDKLHPGMRALDVGSGTGYLTGCFALMVGAEGRVVGVDH 195

Query: 193 IPELVASSIKNIEKSAAAPLLKKGSLSVHVGDGRQGWAECAPYDAIHVGAAAAEIPPALI 252
           IPELV  SIKNIEKS AA  LKKGSLS+HVGDGR+GW E APYDAIHVGAAA+EIP  L+
Sbjct: 196 IPELVDMSIKNIEKSVAASFLKKGSLSLHVGDGRKGWQEFAPYDAIHVGAAASEIPQPLL 255

Query: 253 DQLKPGGRMVIPVGNIFQDLKVVDKDTDGSINIHDETSVRYVPLTSREAQLG 305
           DQLKPGGRMVIP+G  FQ+LKV+DK+ DGSI +H ETSVRYVPLTSR  QLG
Sbjct: 256 DQLKPGGRMVIPLGTYFQELKVIDKNEDGSIKVHTETSVRYVPLTSRVEQLG 307

BLAST of CmaCh10G000820.1 vs. NCBI nr
Match: gi|659129028|ref|XP_008464492.1| (PREDICTED: protein-L-isoaspartate O-methyltransferase 1 isoform X1 [Cucumis melo])

HSP 1 Score: 537.3 bits (1383), Expect = 1.7e-149
Identity = 270/315 (85.71%), Postives = 290/315 (92.06%), Query Frame = 1

Query: 1   MNLTQISPSL--------RYCAPSIGLYFNTPSSLRRHRRLPLRLPTALPPTASSVPKTT 60
           M++TQIS SL        RYCAPS+GL+FN     RR RR   RLPTALPPT SSVPKTT
Sbjct: 1   MSITQISLSLSFPLAYASRYCAPSLGLFFNLSCHHRRRRR---RLPTALPPTTSSVPKTT 60

Query: 61  -TTIIPFLPRFLNPHFVTGNCLLSRMERFWSGSGIGTNKEMVENLQSYGVVRSKKVSEVM 120
            TTII FLPRFL+PHF+TGNCLLSRMERFW+GSGIGTNK+MVE LQ+YGVVRSKKVSEVM
Sbjct: 61  VTTIISFLPRFLDPHFLTGNCLLSRMERFWTGSGIGTNKDMVEKLQTYGVVRSKKVSEVM 120

Query: 121 ESVDRAFFVPDGVPPYVDTPLPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTG 180
           E++DRAFFVPD VPPYVDTP+PIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTG
Sbjct: 121 ENIDRAFFVPDDVPPYVDTPVPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTG 180

Query: 181 YLTACFALMVGPEGRAVGVEHIPELVASSIKNIEKSAAAPLLKKGSLSVHVGDGRQGWAE 240
           YLTACFALMVGPEGR VGVEHIPELVASS++NI+KSAAAPLLK+GSLS+HVGDGRQGWAE
Sbjct: 181 YLTACFALMVGPEGRVVGVEHIPELVASSMENIKKSAAAPLLKEGSLSLHVGDGRQGWAE 240

Query: 241 CAPYDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQDLKVVDKDTDGSINIHDETSV 300
           CAPYDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQDLKVVDKD+DGS++IHDETSV
Sbjct: 241 CAPYDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQDLKVVDKDSDGSVSIHDETSV 300

Query: 301 RYVPLTSREAQLGDN 307
           RYVPLTSREAQL DN
Sbjct: 301 RYVPLTSREAQLRDN 312

BLAST of CmaCh10G000820.1 vs. NCBI nr
Match: gi|778655443|ref|XP_011653620.1| (PREDICTED: protein-L-isoaspartate O-methyltransferase 1 isoform X2 [Cucumis sativus])

HSP 1 Score: 533.9 bits (1374), Expect = 1.9e-148
Identity = 267/313 (85.30%), Postives = 289/313 (92.33%), Query Frame = 1

Query: 1   MNLTQISPSL------RYCAPSIGLYFNTPSSLRRHRRLPLRLPTALPPTASSVPKTT-T 60
           M++TQIS S       RYCAPS+GL+FN   SL R RR P  L TALPPT SSVPKTT T
Sbjct: 1   MSITQISLSFPLAYASRYCAPSLGLFFNL--SLHRRRRRPRLLSTALPPTTSSVPKTTVT 60

Query: 61  TIIPFLPRFLNPHFVTGNCLLSRMERFWSGSGIGTNKEMVENLQSYGVVRSKKVSEVMES 120
           TI+ FLPRFL+PHF+TGNCLLSRMERFWSGSGIGTNK+MVE LQ+YGVVRSK+VSEVMES
Sbjct: 61  TIVSFLPRFLDPHFLTGNCLLSRMERFWSGSGIGTNKDMVEKLQTYGVVRSKRVSEVMES 120

Query: 121 VDRAFFVPDGVPPYVDTPLPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYL 180
           +DRAFFVPD VPPYVDTP+PIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYL
Sbjct: 121 IDRAFFVPDDVPPYVDTPVPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYL 180

Query: 181 TACFALMVGPEGRAVGVEHIPELVASSIKNIEKSAAAPLLKKGSLSVHVGDGRQGWAECA 240
           TACFALMVGPEGR VGVEHIPELVASS++NI+KSAAAPLLK+GSLS+HVGDGRQGWAECA
Sbjct: 181 TACFALMVGPEGRVVGVEHIPELVASSMENIKKSAAAPLLKEGSLSLHVGDGRQGWAECA 240

Query: 241 PYDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQDLKVVDKDTDGSINIHDETSVRY 300
           PYDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGN+FQDLKVVDKD+DGS++IHDETSVRY
Sbjct: 241 PYDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGNVFQDLKVVDKDSDGSVSIHDETSVRY 300

Query: 301 VPLTSREAQLGDN 307
           VPLTSREAQL D+
Sbjct: 301 VPLTSREAQLRDD 311

BLAST of CmaCh10G000820.1 vs. NCBI nr
Match: gi|778655440|ref|XP_011653592.1| (PREDICTED: protein-L-isoaspartate O-methyltransferase 1 isoform X1 [Cucumis sativus])

HSP 1 Score: 522.3 bits (1344), Expect = 5.6e-145
Identity = 267/332 (80.42%), Postives = 289/332 (87.05%), Query Frame = 1

Query: 1   MNLTQISPSL------RYCAPSIGLYFNTPSSLRRHRRLPLRLPTALPPTASSVPKTT-T 60
           M++TQIS S       RYCAPS+GL+FN   SL R RR P  L TALPPT SSVPKTT T
Sbjct: 1   MSITQISLSFPLAYASRYCAPSLGLFFNL--SLHRRRRRPRLLSTALPPTTSSVPKTTVT 60

Query: 61  TIIPFLPRFLNPHFVTGNCLLSRME-------------------RFWSGSGIGTNKEMVE 120
           TI+ FLPRFL+PHF+TGNCLLSRME                   RFWSGSGIGTNK+MVE
Sbjct: 61  TIVSFLPRFLDPHFLTGNCLLSRMEILGFLKMDKLPPRSFTTLKRFWSGSGIGTNKDMVE 120

Query: 121 NLQSYGVVRSKKVSEVMESVDRAFFVPDGVPPYVDTPLPIGYNATISAPHMHATCLQLLE 180
            LQ+YGVVRSK+VSEVMES+DRAFFVPD VPPYVDTP+PIGYNATISAPHMHATCLQLLE
Sbjct: 121 KLQTYGVVRSKRVSEVMESIDRAFFVPDDVPPYVDTPVPIGYNATISAPHMHATCLQLLE 180

Query: 181 KHLQPGMRALDVGSGTGYLTACFALMVGPEGRAVGVEHIPELVASSIKNIEKSAAAPLLK 240
           KHLQPGMRALDVGSGTGYLTACFALMVGPEGR VGVEHIPELVASS++NI+KSAAAPLLK
Sbjct: 181 KHLQPGMRALDVGSGTGYLTACFALMVGPEGRVVGVEHIPELVASSMENIKKSAAAPLLK 240

Query: 241 KGSLSVHVGDGRQGWAECAPYDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQDLKV 300
           +GSLS+HVGDGRQGWAECAPYDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGN+FQDLKV
Sbjct: 241 EGSLSLHVGDGRQGWAECAPYDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGNVFQDLKV 300

Query: 301 VDKDTDGSINIHDETSVRYVPLTSREAQLGDN 307
           VDKD+DGS++IHDETSVRYVPLTSREAQL D+
Sbjct: 301 VDKDSDGSVSIHDETSVRYVPLTSREAQLRDD 330

BLAST of CmaCh10G000820.1 vs. NCBI nr
Match: gi|659129030|ref|XP_008464493.1| (PREDICTED: protein-L-isoaspartate O-methyltransferase 1 isoform X2 [Cucumis melo])

HSP 1 Score: 436.8 bits (1122), Expect = 3.1e-119
Identity = 210/230 (91.30%), Postives = 226/230 (98.26%), Query Frame = 1

Query: 77  MERFWSGSGIGTNKEMVENLQSYGVVRSKKVSEVMESVDRAFFVPDGVPPYVDTPLPIGY 136
           ++RFW+GSGIGTNK+MVE LQ+YGVVRSKKVSEVME++DRAFFVPD VPPYVDTP+PIGY
Sbjct: 12  LKRFWTGSGIGTNKDMVEKLQTYGVVRSKKVSEVMENIDRAFFVPDDVPPYVDTPVPIGY 71

Query: 137 NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMVGPEGRAVGVEHIPEL 196
           NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMVGPEGR VGVEHIPEL
Sbjct: 72  NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMVGPEGRVVGVEHIPEL 131

Query: 197 VASSIKNIEKSAAAPLLKKGSLSVHVGDGRQGWAECAPYDAIHVGAAAAEIPPALIDQLK 256
           VASS++NI+KSAAAPLLK+GSLS+HVGDGRQGWAECAPYDAIHVGAAAAEIPPALIDQLK
Sbjct: 132 VASSMENIKKSAAAPLLKEGSLSLHVGDGRQGWAECAPYDAIHVGAAAAEIPPALIDQLK 191

Query: 257 PGGRMVIPVGNIFQDLKVVDKDTDGSINIHDETSVRYVPLTSREAQLGDN 307
           PGGRMVIPVGNIFQDLKVVDKD+DGS++IHDETSVRYVPLTSREAQL DN
Sbjct: 192 PGGRMVIPVGNIFQDLKVVDKDSDGSVSIHDETSVRYVPLTSREAQLRDN 241

BLAST of CmaCh10G000820.1 vs. NCBI nr
Match: gi|661881352|emb|CDP15099.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 413.7 bits (1062), Expect = 2.8e-112
Identity = 214/301 (71.10%), Postives = 235/301 (78.07%), Query Frame = 1

Query: 6   ISPSLRYCAP---SIGLYFNTPSSLRRHRRLPLRLPTALPPTASSVPKTTTTIIPFLPRF 65
           I+   RY AP   S   +     SL  H R   RLP   P + + +   TT       R 
Sbjct: 20  IACGCRYRAPLKHSFLAFTRRFHSLHPHHRYCRRLP---PHSLAILAANTTAYSSRSRRL 79

Query: 66  LNPHFVTGNCLLSRMERFWSGSGIGTNKEMVENLQSYGVVRSKKVSEVMESVDRAFFVPD 125
              HF TGN L SRMERFW+G GI  N+ MVE+LQSYGVVRSKKV+EVM ++DRA FVPD
Sbjct: 80  PKTHFFTGNSLFSRMERFWTGGGIDKNRAMVEHLQSYGVVRSKKVAEVMGTIDRALFVPD 139

Query: 126 GVPPYVDTPLPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMVG 185
           G PPYVDTP+ IGYNATISAPHMHA CL+LLE HLQPGM ALD+GSGTGYLTACFA+MVG
Sbjct: 140 GTPPYVDTPMQIGYNATISAPHMHAMCLELLENHLQPGMHALDIGSGTGYLTACFAIMVG 199

Query: 186 PEGRAVGVEHIPELVASSIKNIEKSAAAPLLKKGSLSVHVGDGRQGWAECAPYDAIHVGA 245
           P+G AVGVEHIPEL A SIKNI+KSAAAPLLK+GSLS+H GDGR GW E APYDAIHVGA
Sbjct: 200 PQGHAVGVEHIPELAAESIKNIQKSAAAPLLKEGSLSIHAGDGRLGWPEHAPYDAIHVGA 259

Query: 246 AAAEIPPALIDQLKPGGRMVIPVGNIFQDLKVVDKDTDGSINIHDETSVRYVPLTSREAQ 304
           AA EIP ALIDQLKPGGRMVIPVGNIFQDLKVVDKD DGSI++H ETSVRYVPLTSREAQ
Sbjct: 260 AAPEIPQALIDQLKPGGRMVIPVGNIFQDLKVVDKDMDGSISVHSETSVRYVPLTSREAQ 317

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PIMT1_ARATH2.1e-9977.97Protein-L-isoaspartate O-methyltransferase 1 OS=Arabidopsis thaliana GN=PIMT1 PE... [more]
PIMT2_ARATH3.1e-9574.14Protein-L-isoaspartate O-methyltransferase 2 OS=Arabidopsis thaliana GN=PIMT2 PE... [more]
PIMT_WHEAT4.7e-9168.70Protein-L-isoaspartate O-methyltransferase OS=Triticum aestivum GN=PCM PE=1 SV=1[more]
PIMT_CHICK2.9e-5652.94Protein-L-isoaspartate(D-aspartate) O-methyltransferase OS=Gallus gallus GN=PCMT... [more]
PIMT_PIG1.1e-5552.49Protein-L-isoaspartate(D-aspartate) O-methyltransferase OS=Sus scrofa GN=PCMT1 P... [more]
Match NameE-valueIdentityDescription
A0A0A0LRC6_CUCSA1.3e-14885.30Protein-L-isoaspartate O-methyltransferase OS=Cucumis sativus GN=Csa_1G002880 PE... [more]
A0A068V3L9_COFCA2.0e-11271.10Protein-L-isoaspartate O-methyltransferase OS=Coffea canephora GN=GSCOC_T0004266... [more]
A0A061FZ18_THECC4.8e-11170.59Protein-L-isoaspartate O-methyltransferase OS=Theobroma cacao GN=TCM_014514 PE=3... [more]
M5WF21_PRUPE6.3e-11169.26Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa006554mg PE=4 SV=1[more]
V4VKI5_9ROSI1.6e-10975.38Protein-L-isoaspartate O-methyltransferase OS=Citrus clementina GN=CICLE_v100213... [more]
Match NameE-valueIdentityDescription
AT3G48330.11.2e-10077.97 protein-l-isoaspartate methyltransferase 1[more]
AT5G50240.11.8e-9674.14 protein-l-isoaspartate methyltransferase 2[more]
Match NameE-valueIdentityDescription
gi|659129028|ref|XP_008464492.1|1.7e-14985.71PREDICTED: protein-L-isoaspartate O-methyltransferase 1 isoform X1 [Cucumis melo... [more]
gi|778655443|ref|XP_011653620.1|1.9e-14885.30PREDICTED: protein-L-isoaspartate O-methyltransferase 1 isoform X2 [Cucumis sati... [more]
gi|778655440|ref|XP_011653592.1|5.6e-14580.42PREDICTED: protein-L-isoaspartate O-methyltransferase 1 isoform X1 [Cucumis sati... [more]
gi|659129030|ref|XP_008464493.1|3.1e-11991.30PREDICTED: protein-L-isoaspartate O-methyltransferase 1 isoform X2 [Cucumis melo... [more]
gi|661881352|emb|CDP15099.1|2.8e-11271.10unnamed protein product [Coffea canephora][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR000682PCMT
Vocabulary: Molecular Function
TermDefinition
GO:0004719protein-L-isoaspartate (D-aspartate) O-methyltransferase activity
Vocabulary: Biological Process
TermDefinition
GO:0006464cellular protein modification process
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006464 cellular protein modification process
biological_process GO:0007568 aging
biological_process GO:0006479 protein methylation
biological_process GO:0009651 response to salt stress
biological_process GO:0046500 S-adenosylmethionine metabolic process
biological_process GO:0009845 seed germination
cellular_component GO:0005829 cytosol
molecular_function GO:0004719 protein-L-isoaspartate (D-aspartate) O-methyltransferase activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmaCh10G000820CmaCh10G000820gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmaCh10G000820.1CmaCh10G000820.1-proteinpolypeptide


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh10G000820.1.exon.4CmaCh10G000820.1.exon.4exon
CmaCh10G000820.1.exon.3CmaCh10G000820.1.exon.3exon
CmaCh10G000820.1.exon.2CmaCh10G000820.1.exon.2exon
CmaCh10G000820.1.exon.1CmaCh10G000820.1.exon.1exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh10G000820.1.five_prime_UTR.1CmaCh10G000820.1.five_prime_UTR.1five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh10G000820.1.CDS.1CmaCh10G000820.1.CDS.1CDS
CmaCh10G000820.1.CDS.2CmaCh10G000820.1.CDS.2CDS
CmaCh10G000820.1.CDS.3CmaCh10G000820.1.CDS.3CDS
CmaCh10G000820.1.CDS.4CmaCh10G000820.1.CDS.4CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh10G000820.1.three_prime_UTR.1CmaCh10G000820.1.three_prime_UTR.1three_prime_UTR


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000682Protein-L-isoaspartate(D-aspartate) O-methyltransferasePANTHERPTHR11579PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASEcoord: 79..303
score: 3.9E
IPR000682Protein-L-isoaspartate(D-aspartate) O-methyltransferaseTIGRFAMsTIGR00080TIGR00080coord: 88..302
score: 1.5
IPR000682Protein-L-isoaspartate(D-aspartate) O-methyltransferasePROSITEPS01279PCMTcoord: 223..238
scor
NoneNo IPR availablePANTHERPTHR11579:SF8SUBFAMILY NOT NAMEDcoord: 79..303
score: 3.9E
NoneNo IPR availablePFAMPF01135PCMTcoord: 89..300
score: 3.9