CmaCh08G004070.1 (mRNA) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh08G004070.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Cell cycle serine/threonine-protein kinase CDC5/MSD2
Location	Cma_Chr08 : 2334305 .. 2338331 (-)
Sequence length	1712

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGGAGAGCGTGTTAAGAGGGGATTGACGAGAGCTTCGTTGCAGATTCCGATGAGGAGAAAATCCAAAACCCTAAGAACGACGATGACCGCAACGAGGAAGAAGATGAAAGGGACATTGACGAGGCACTTGCAGGTCCAGTTAGGGCAGGTTTCTCTCGCCCCTCTCCACCACCAACTTTCCTCTTCCACCGCCGCTTGCTTCCAAGACTTGCCTGAATCTGAAGCAATGGCGACGGTAAACGATTCTGGCGCTCTCCCTTCCTCGTCTACTAACTTTGAGGACAGAGATTTCATTCTTAGTCAGGATTTCTTCTGGTGAGATTTATATCGTTATTTTTGTTTTTCATTTGTGAGTTGATTTCAGCTGCTGTTGATGTTGATTGTTATCTTTCTCGAGGTTCTGGAAATTAGTTTTCTGTTCGAAGTAGTTTGATTGACGGGATAGTGTAGTAAGTGATTGACGAATCACTGAATACTCTTTAGGAGTTACTTCGAGTTTTGGAGTTTTCTACTAGTTTCCCTTGTGAGTTGTTAGGTAATTTGAACGTCTATTGATGGTAGTTGATTATTCGTGTTATATCCCATGGTTTAAGTTGCTTGTCAGTTACGAAATGGAGGTCATTTCCTGTGAAAGTAACTTAGATAGTCAGAAAAATGGAGTATGATTTACTAATTATTGAATGTTTTTAGAAATTACTTTGCTTGTTACTATTGATACTGGTATACAAGGATACCAAAGAGTAAGATTGAAGGCTGCATTGTAACTGGCCAGATCATTTTTGGCGTTTAACGTGGGATTTTGTTTGTATGTTGCAGCACCCCTGACTATATCACTCCGGACAACCAAAATCTGCTGAATGGCGTGGATTGCTACAAGGTGAGCAGTTAATGGAGAAAGATAACCCTGTCCATAACAATGGGTTTTCAAAAGGAAAAGAAGAAGAAGAAGAAGAAGAAAATAATGTGAATTCTCTAATTTTCTGGGTGTCCTTGTCTTGGCAGGAAAGCACTCCTTGCCCCAAATCTCCAGAGAAGATTAATACTGTTAAAACTAAGAGAAAAATACGTGGTAAGTTTATATAGTGGTGATCTCGAACTTTAATCTGGGAGTGTTATAAGAGAACAGAAAGTCACATGCATTAGTTCAGTGGCATATGCTCAATTATGATCATGTCGAAACTCAACTTTCCTAATCAATAATATATATGGTTAGTTGCTTGCATAAAACTATGACCAGTATCTGAATCATTTATCAATGTGGAATTTAAGACATGGATCTGAAATTATTTTGGGGCGTCAAAATCTGACAACATCATGTTCCTTTTCAATATTAGTTACATTAATTGTTGATTGATGTAGTATAATTCATTTTCTTCATTGAATGTTCATTTGTCACAGACGGTGTTTTAGTACATCCTCTGAGTCCAAGTTTGTCTGGCCGTGAACAAGTTGTGGAGCTAGGAAATGACACTTTTGGAACAGATGATGAAGATATGGAGAAAGAGACAATGGCAATGCCAAAGAAACAAAACTATGTTTCTCAATCTGCTGTTGCTTTACGCTGTCGAGTTATGCCTCCTCCTTGCATGAAGAATCCATACTTGAATGATGCTTCAGACATAGGTGTAGACCCATTTGGTAATCAACGAACAAAATGTGCAGGTACAGTCCAATTAATTCTTAAAGACTAATAAAACATTTTTTGAAGTCGTCTTTTTTAACGAGGACTTTATGTTTAATGAATTAGTTTCTTGGTTTTAATTTTGTGTAGATTTTTTTCCCGCACTGTTTAGTGGAGATGGTCTTTCAAGATATCACACCGATTTTCATGAGATAAAGGTATCTAGAAACATCTGCTTTGTTTGCAAGCTTCTGCAAATAGATTGGTTTTTTCTTTTATTTATATTTCAATTCAGGCTGTTGTCAAGTTTTATATTAACCTCAGTCTATTTCCAGCAAATAGGAACTGGAAATTTTAGCCGTGTCTTCAAGGTATTGAAGAGAATTGATGGTTGTTTATATGCTGTTAAACAAAGCACAAGATCATTGCATCAAGACATAGAAAGGTTACTTCTTTTGGTGTGTCCGTATATGTTTAACCTCTAAACACTAGCAAATGTGTATTTGAAGTTTTTCCTTCCATTTGCAGGAGGAGAGCTTTGATGGAAGTTCAAGCTTTGGCAGCTTTAGGTTAGTAAATATATTTAGATATGATTTTCTGTTTTATTATATCTCTATTTCTATTGTAGTTATTATTTCTCTCTTGAGCAACTTGTTTCTTTTCCCGACTGTTGGCCACTATTTAACGTGTAGGATCTCATGAAAACATCGTTGGATACTACTCTTCGTGGTTTGAAAATGAGCAACTCTACATTCAAATGGAACTCTGTGATTGTAGTTTATCCACTGGCAGGTATTCTCACCCGTTCTCTGAGGCTGATGCACTGAAAGCCTTGCATCAGGTGAGATTTCGAGTTGCTTCGCTCATCTCCCTGATTTGTTGTCTTCAAGAGTTAATTAGTTAGTTCTGCCCTTCATTCAATACGTAGCTTTTAATAGGAAATGCCACTTCTGAAAAATAATTATCTCAGAATTTTGTGTAATTTCAACTTTATCCATTCCAATTTTCAAGTCCTAGAACTAGGAACTTTTTAAATTTCATGGCTTCTATGCTGACAATGAACTACAAACAGATTGCAAAAGCATTGCTGTTTATGCACGAGAAAGGAGTAGCTCATTTAGACGTGAAACCTGACAATATTTATGTCAAAGATGGTGTTTATAAACTTGGTGATTTTGGATGCGCTACTCTTCTTGATAAAAGCCTACCAATTGAGGAGGGCGATGCACGCTATATGCCTCAAGAAATTCTGAATGAGAGATACGATTATCTTGACAAGGTTGATATATTCTCTTTGGGAGCTGCTATTTATGAGGTTGTGAGAGGTTCGACGTTTCCAGAAACACGTTTCATGAATCTAAAGGAGGGAAAATTGCCACTCTTTCCTTGTCATTCATTGCAATTTCAAAACTTAATCAAGGTACTGAATGCGTAATTGTGTTTTCTTTTCATTTCATTTCCATCGACTATTCTTCTGACTTGACCTGACGTGAGATCCTACCGGTTGGAGAGGAGAACGAGACATTCTTTACAAAGGTTTATAAAGGTGTAGAAACCTCTCCCTGATAGATGCGTTTTAAAACCTTAAGGGGAAGCCCAGAAGAGAAAGCCCAAAGAGGATAATATCTATTAGTGGTGGGCTTATACTGTTAAAAATGGTATCAGAGCCGATTATCGAGCGAGTGGTGTTCCAGTAGGAACGCTTGACCCTCAAGGGAGTGGATTATGAGATCCCACATCAGTTGGAGAGGGGAACGAAATATTTTTTATTAAGATGTTGAAACCTTTTCCAGACGCGTTTTAAAACTGTGAGAGAAAGTCCAAAGAGTAGATAATATTTGTTAGCCCGATCTTAGTCTTTGACTCACCTATGCAGAGGAAATCTTAGATTTTTGGGGCATTTGATTCTTACCTATTTTTGCATTTTTTTTTCCTACAAATTCTACTTTCCATAAAAAAAAAATGTAAAAAAGTGAGTCAAAACTAGCCTTGGTACAAACATGATTTCGTATCGACTTTGTGTGACCACAAGTAATGTTCACTGTTTACATCATTATCTTGGAAGTTCATTAGAGATAAGATAGCTAATTTTTCTGATTAATTGACAACTCTAACAGGCGATGGTGGATCCCGACCCAGCACGACGGCCTTCAGCTAGGGAAATTCTTCAGAATCCCATATTTGATAAGGTCAGAAATCATCCAAAATCATAGGTCAACTGTGGGTTAGTGTGTTTAATCACTTGCATTAGCCAGCTATTTTTGTAAGATTTCAATCACCACTTTCCCAAGTTCATGCATCAAATTTTGTCTCTAGCATGGTGCAAATTTTGTAAATTCTCTATGATAATATTGTATCATAAACTCGTAAATGCAAGTTAACGAAAGCTCTTTCATTTCCAAATA

mRNA sequence

GAGGAGAGCGTGTTAAGAGGGGATTGACGAGAGCTTCGTTGCAGATTCCGATGAGGAGAAAATCCAAAACCCTAAGAACGACGATGACCGCAACGAGGAAGAAGATGAAAGGGACATTGACGAGGCACTTGCAGGTCCAGTTAGGGCAGGTTTCTCTCGCCCCTCTCCACCACCAACTTTCCTCTTCCACCGCCGCTTGCTTCCAAGACTTGCCTGAATCTGAAGCAATGGCGACGGTAAACGATTCTGGCGCTCTCCCTTCCTCGTCTACTAACTTTGAGGACAGAGATTTCATTCTTAGTCAGGATTTCTTCTGCACCCCTGACTATATCACTCCGGACAACCAAAATCTGCTGAATGGCGTGGATTGCTACAAGGAAAGCACTCCTTGCCCCAAATCTCCAGAGAAGATTAATACTGTTAAAACTAAGAGAAAAATACGTGACGGTGTTTTAGTACATCCTCTGAGTCCAAGTTTGTCTGGCCGTGAACAAGTTGTGGAGCTAGGAAATGACACTTTTGGAACAGATGATGAAGATATGGAGAAAGAGACAATGGCAATGCCAAAGAAACAAAACTATGTTTCTCAATCTGCTGTTGCTTTACGCTGTCGAGTTATGCCTCCTCCTTGCATGAAGAATCCATACTTGAATGATGCTTCAGACATAGGTGTAGACCCATTTGGTAATCAACGAACAAAATGTGCAGATTTTTTTCCCGCACTGTTTAGTGGAGATGGTCTTTCAAGATATCACACCGATTTTCATGAGATAAAGCAAATAGGAACTGGAAATTTTAGCCGTGTCTTCAAGGTATTGAAGAGAATTGATGGTTGTTTATATGCTGTTAAACAAAGCACAAGATCATTGCATCAAGACATAGAAAGGAGGAGAGCTTTGATGGAAGTTCAAGCTTTGGCAGCTTTAGGATCTCATGAAAACATCGTTGGATACTACTCTTCGTGGTTTGAAAATGAGCAACTCTACATTCAAATGGAACTCTGTGATTGTAGTTTATCCACTGGCAGGTATTCTCACCCGTTCTCTGAGGCTGATGCACTGAAAGCCTTGCATCAGATTGCAAAAGCATTGCTGTTTATGCACGAGAAAGGAGTAGCTCATTTAGACGTGAAACCTGACAATATTTATGTCAAAGATGGTGTTTATAAACTTGGTGATTTTGGATGCGCTACTCTTCTTGATAAAAGCCTACCAATTGAGGAGGGCGATGCACGCTATATGCCTCAAGAAATTCTGAATGAGAGATACGATTATCTTGACAAGGTTGATATATTCTCTTTGGGAGCTGCTATTTATGAGGTTGTGAGAGGTTCGACGTTTCCAGAAACACGTTTCATGAATCTAAAGGAGGGAAAATTGCCACTCTTTCCTTGTCATTCATTGCAATTTCAAAACTTAATCAAGGCGATGGTGGATCCCGACCCAGCACGACGGCCTTCAGCTAGGGAAATTCTTCAGAATCCCATATTTGATAAGGTCAGAAATCATCCAAAATCATAGGTCAACTGTGGGTTAGTGTGTTTAATCACTTGCATTAGCCAGCTATTTTTGTAAGATTTCAATCACCACTTTCCCAAGTTCATGCATCAAATTTTGTCTCTAGCATGGTGCAAATTTTGTAAATTCTCTATGATAATATTGTATCATAAACTCGTAAATGCAAGTTAACGAAAGCTCTTTCATTTCCAAATA

Coding sequence (CDS)

ATGAGGAGAAAATCCAAAACCCTAAGAACGACGATGACCGCAACGAGGAAGAAGATGAAAGGGACATTGACGAGGCACTTGCAGGTCCAGTTAGGGCAGGTTTCTCTCGCCCCTCTCCACCACCAACTTTCCTCTTCCACCGCCGCTTGCTTCCAAGACTTGCCTGAATCTGAAGCAATGGCGACGGTAAACGATTCTGGCGCTCTCCCTTCCTCGTCTACTAACTTTGAGGACAGAGATTTCATTCTTAGTCAGGATTTCTTCTGCACCCCTGACTATATCACTCCGGACAACCAAAATCTGCTGAATGGCGTGGATTGCTACAAGGAAAGCACTCCTTGCCCCAAATCTCCAGAGAAGATTAATACTGTTAAAACTAAGAGAAAAATACGTGACGGTGTTTTAGTACATCCTCTGAGTCCAAGTTTGTCTGGCCGTGAACAAGTTGTGGAGCTAGGAAATGACACTTTTGGAACAGATGATGAAGATATGGAGAAAGAGACAATGGCAATGCCAAAGAAACAAAACTATGTTTCTCAATCTGCTGTTGCTTTACGCTGTCGAGTTATGCCTCCTCCTTGCATGAAGAATCCATACTTGAATGATGCTTCAGACATAGGTGTAGACCCATTTGGTAATCAACGAACAAAATGTGCAGATTTTTTTCCCGCACTGTTTAGTGGAGATGGTCTTTCAAGATATCACACCGATTTTCATGAGATAAAGCAAATAGGAACTGGAAATTTTAGCCGTGTCTTCAAGGTATTGAAGAGAATTGATGGTTGTTTATATGCTGTTAAACAAAGCACAAGATCATTGCATCAAGACATAGAAAGGAGGAGAGCTTTGATGGAAGTTCAAGCTTTGGCAGCTTTAGGATCTCATGAAAACATCGTTGGATACTACTCTTCGTGGTTTGAAAATGAGCAACTCTACATTCAAATGGAACTCTGTGATTGTAGTTTATCCACTGGCAGGTATTCTCACCCGTTCTCTGAGGCTGATGCACTGAAAGCCTTGCATCAGATTGCAAAAGCATTGCTGTTTATGCACGAGAAAGGAGTAGCTCATTTAGACGTGAAACCTGACAATATTTATGTCAAAGATGGTGTTTATAAACTTGGTGATTTTGGATGCGCTACTCTTCTTGATAAAAGCCTACCAATTGAGGAGGGCGATGCACGCTATATGCCTCAAGAAATTCTGAATGAGAGATACGATTATCTTGACAAGGTTGATATATTCTCTTTGGGAGCTGCTATTTATGAGGTTGTGAGAGGTTCGACGTTTCCAGAAACACGTTTCATGAATCTAAAGGAGGGAAAATTGCCACTCTTTCCTTGTCATTCATTGCAATTTCAAAACTTAATCAAGGCGATGGTGGATCCCGACCCAGCACGACGGCCTTCAGCTAGGGAAATTCTTCAGAATCCCATATTTGATAAGGTCAGAAATCATCCAAAATCATAG

Protein sequence

MRRKSKTLRTTMTATRKKMKGTLTRHLQVQLGQVSLAPLHHQLSSSTAACFQDLPESEAMATVNDSGALPSSSTNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDCYKESTPCPKSPEKINTVKTKRKIRDGVLVHPLSPSLSGREQVVELGNDTFGTDDEDMEKETMAMPKKQNYVSQSAVALRCRVMPPPCMKNPYLNDASDIGVDPFGNQRTKCADFFPALFSGDGLSRYHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRSLHQDIERRRALMEVQALAALGSHENIVGYYSSWFENEQLYIQMELCDCSLSTGRYSHPFSEADALKALHQIAKALLFMHEKGVAHLDVKPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEVVRGSTFPETRFMNLKEGKLPLFPCHSLQFQNLIKAMVDPDPARRPSAREILQNPIFDKVRNHPKS

Homology

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BLAST of CmaCh08G004070.1 vs. Swiss-Prot
Match: WEE1_ARATH (Wee1-like protein kinase OS=Arabidopsis thaliana GN=WEE1 PE=1 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 3.9e-164
Identity = 293/488 (60.04%), Postives = 369/488 (75.61%), Query Frame = 1


Query: 2   RRKSKTLRTTMTATRKKMKGTLTRHLQVQLGQVSLAPLHHQLSSSTAA-CFQDLPESEAM 61
           R+   T++T  +   +KM+GTL RH  +Q GQ+S     ++ SS+ A+  FQ L +S++ 
Sbjct: 13  RKTQGTIKTRASKKIRKMEGTLERHSLLQFGQLSKISFENRPSSNVASSAFQGLLDSDSS 72

Query: 62  ATVNDSGALPSSSTNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDCYKESTPCPKSPEK 121
              N  G+  S + N  ++DFILSQDFFCTPDYITPDNQNL++G+D  K+ +PCP+SP K
Sbjct: 73  ELRNQLGSADSDA-NCGEKDFILSQDFFCTPDYITPDNQNLMSGLDISKDHSPCPRSPVK 132

Query: 122 INTVKTKRKIRDGVLVHPLSPSLSGREQVVELGNDTFGTDDEDMEKETMAMPKKQNYVSQ 181
           +NTVK+KR  ++    +  + + S + +V E  ND   TD+   +K      ++  YVSQ
Sbjct: 133 LNTVKSKRCRQESFTGNHSNSTWSSKHRVDEQENDDIDTDEVMGDKLQANQTERTGYVSQ 192

Query: 182 SAVALRCRVMPPPCMKNPYLNDASDIGVDPFGNQRTKCADFFPALFSGDGLSRYHTDFHE 241
           +AVALRCR MPPPC+KNPY+ + S+   DPFG+QR+KCA F P   SGDGLSRY TDFHE
Sbjct: 193 AAVALRCRAMPPPCLKNPYVLNQSETATDPFGHQRSKCASFLPVSTSGDGLSRYLTDFHE 252

Query: 242 IKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRSLHQDIERRRALMEVQALAALGSHENIVG 301
           I+QIG G+FSRVFKVLKR+DGCLYAVK STR L+ D ERR+A+MEVQALAALG HENIVG
Sbjct: 253 IRQIGAGHFSRVFKVLKRMDGCLYAVKHSTRKLYLDSERRKAMMEVQALAALGFHENIVG 312

Query: 302 YYSSWFENEQLYIQMELCDCSLST--GRYSHPFSEADALKALHQIAKALLFMHEKGVAHL 361
           YYSSWFENEQLYIQ+ELCD SLS    + S   SE + L  +HQIAKAL F+HEKG+AHL
Sbjct: 313 YYSSWFENEQLYIQLELCDHSLSALPKKSSLKVSEREILVIMHQIAKALHFVHEKGIAHL 372

Query: 362 DVKPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLG 421
           DVKPDNIY+K+GV KLGDFGCAT LDKSLP+EEGDARYMPQEILNE Y++LDKVDIFSLG
Sbjct: 373 DVKPDNIYIKNGVCKLGDFGCATRLDKSLPVEEGDARYMPQEILNEDYEHLDKVDIFSLG 432

Query: 422 AAIYEVVRGSTFPETR--FMNLKEGKLPLFPCHSLQFQNLIKAMVDPDPARRPSAREILQ 481
             +YE+++GS   E+R   +N+KEGKLPL P HSLQ Q L+K M+D DP RRPSARE+L 
Sbjct: 433 VTVYELIKGSPLTESRNQSLNIKEGKLPLLPGHSLQLQQLLKTMMDRDPKRRPSARELLD 492

Query: 482 NPIFDKVR 485
           +P+FD++R
Sbjct: 493 HPMFDRIR 499

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BLAST of CmaCh08G004070.1 vs. Swiss-Prot
Match: WEE1_ORYSJ (Wee1-like protein kinase OS=Oryza sativa subsp. japonica GN=WEE1 PE=3 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 2.4e-137
Identity = 252/437 (57.67%), Postives = 325/437 (74.37%), Query Frame = 1


Query: 54  LPESEAMATVNDSGALPSSSTNFEDRDF-ILSQDFFCTPDYITPDNQNLLNGVDCYKEST 113
           LP  +  AT  D+  +       E++D  ILSQDFFCTPDYITPD   L +G D  KE+ 
Sbjct: 91  LPPLQPQATPMDADEV------VEEKDSGILSQDFFCTPDYITPDAPQLGSGFDANKENI 150

Query: 114 PCPKSPEKINTVKTKRKIRDGVLVHPLSPSLSGREQVVELGNDTFGTDDEDMEKETMAMP 173
           PCP SPEK +  ++KR  RD       S  +   + +  +  +     +E+  KE+ +  
Sbjct: 151 PCPNSPEK-SVCRSKRYKRDCSPKGLGSNDIFDSQWIAPVQFEGLDDSEEEQLKESSSH- 210

Query: 174 KKQNYVSQSAVALRCRVMPPPCMKNPYLNDASDIGVDPFGNQRTKCADFFPALFSGDGLS 233
           K+ +YVSQSAVALRCRVMPPPC++NPYLN    I  + FG ++ K + F P++  GDGLS
Sbjct: 211 KRGSYVSQSAVALRCRVMPPPCIRNPYLNTDHQIDDNVFGGRQCKSSGFSPSV-DGDGLS 270

Query: 234 RYHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRSLHQDIERRRALMEVQALAAL 293
           RY TDFHEI+QIG GNFS VFKVLKRIDGCLYAVK+S R LH D ERR+A+ EVQALAAL
Sbjct: 271 RYRTDFHEIEQIGRGNFSVVFKVLKRIDGCLYAVKRSIRQLHNDRERRQAVKEVQALAAL 330

Query: 294 GSHENIVGYYSSWFENEQLYIQMELCDCSLSTGRYSHPFSEADALKALHQIAKALLFMHE 353
           G HENIVGY++SWFEN+QL+IQMELCD  LS  R + P    +AL+ L+QI K L F+HE
Sbjct: 331 GCHENIVGYFTSWFENKQLFIQMELCDRCLSMDR-NQPLKCGEALELLYQICKGLDFIHE 390

Query: 354 KGVAHLDVKPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKV 413
           +G+AHLDVKPDNIYV++GVYKLGDFGCATL+D+SL IE+GD+RYMP E+LN++Y++LDKV
Sbjct: 391 RGIAHLDVKPDNIYVRNGVYKLGDFGCATLIDRSLAIEDGDSRYMPPEMLNDKYEHLDKV 450

Query: 414 DIFSLGAAIYEVVRGSTFPET--RFMNLKEGKLPLFPCHSLQFQNLIKAMVDPDPARRPS 473
           DIFSLGAAIYE++RG+  P++  +F +L+EGK+ L P   +QFQ+LIK+M+DPDP RRPS
Sbjct: 451 DIFSLGAAIYELIRGTQLPDSGPQFTSLREGKIALLPGCPMQFQSLIKSMMDPDPVRRPS 510

Query: 474 AREILQNPIFDKVRNHP 488
           A+E+L++PIFDK+   P
Sbjct: 511 AKEVLRHPIFDKLHKAP 517

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BLAST of CmaCh08G004070.1 vs. Swiss-Prot
Match: WEE1B_XENLA (Wee1-like protein kinase 1-B OS=Xenopus laevis GN=wee1-b PE=1 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 2.7e-48
Identity = 99/277 (35.74%), Postives = 157/277 (56.68%), Query Frame = 1


Query: 232 SRYHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRSLHQDIERRRALMEVQALAA 291
           SRY T+FHE+++IG+G F  VFK +KR+DGC+YA+K+S + L   ++ + AL EV A A 
Sbjct: 242 SRYATEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAV 301

Query: 292 LGSHENIVGYYSSWFENEQLYIQMELC------DCSLSTGRYSHPFSEADALKALHQIAK 351
           LG H ++V YYS+W E++ + IQ E C      D      R    F+E +    L Q+A+
Sbjct: 302 LGQHPHVVRYYSAWAEDDHMLIQNEYCNGGSLSDVISENYRTMQYFTEPELKDLLLQVAR 361

Query: 352 ALLFMHEKGVAHLDVKPDNIYVK--------------------DGVYKLGDFGCATLLDK 411
            L ++H   + H+D+KP NI++                       +YK+GD G  T +  
Sbjct: 362 GLKYIHSMSLVHMDIKPSNIFISRTTLPNTAVEEADDEECGSGKVIYKIGDLGHVTRV-S 421

Query: 412 SLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEVVRGSTFPET--RFMNLKEGKL 471
           S  +EEGD+R++  E+L E Y +L K DIF+L   ++       FP    ++  +++GKL
Sbjct: 422 SPQVEEGDSRFLANEVLQENYTHLAKADIFALALTVWSAAGAEPFPTNGDQWHEIRQGKL 481

Query: 472 PLFP-CHSLQFQNLIKAMVDPDPARRPSAREILQNPI 480
           P  P   S +F +LIK M+ PDP +RPS+  ++++ +
Sbjct: 482 PRVPQLLSQEFVDLIKLMISPDPEKRPSSVALVKHSV 517

[Close]

BLAST of CmaCh08G004070.1 vs. Swiss-Prot
Match: WEE1_DROME (Wee1-like protein kinase OS=Drosophila melanogaster GN=Wee1 PE=1 SV=2)

HSP 1 Score: 191.4 bits (485), Expect = 2.3e-47
Identity = 105/295 (35.59%), Postives = 157/295 (53.22%), Query Frame = 1


Query: 231 LSRYHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRSLHQDIERRRALMEVQALA 290
           +SR+  +F ++  IG G F  VF+ + R+DGC+YA+K+S + +      +RAL EV A A
Sbjct: 232 ISRFKREFMQVNVIGVGEFGVVFQCVNRLDGCIYAIKKSKKPVAGSSFEKRALNEVWAHA 291

Query: 291 ALGSHENIVGYYSSWFENEQLYIQMELCDC-SLSTGRYSHPFSEADALKALHQIAKALLF 350
            LG H+N+V YYS+W E++ + IQ E CD  SL      H   EA+    L  + + L +
Sbjct: 292 VLGKHDNVVRYYSAWAEDDHMLIQNEFCDGGSLHARIQDHCLGEAELKIVLMHVIEGLRY 351

Query: 351 MHEKGVAHLDVKPDNIYVK-------------------DGV---------------YKLG 410
           +H   + H+D+KP+NI+                     DG+               YK+G
Sbjct: 352 IHSNDLVHMDLKPENIFSTMNPNAHKLVEVQPQQTKDDDGMDSVYEELRHSENLVTYKIG 411

Query: 411 DFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEVVRGSTFPET-- 470
           D G  T + K   +EEGD RY+P+EIL+E Y  L K DIFSLG  ++E   G   P+   
Sbjct: 412 DLGHVTSV-KEPYVEEGDCRYLPKEILHEDYSNLFKADIFSLGITLFEAAGGGPLPKNGP 471

Query: 471 RFMNLKEGKLPLFPCHSLQFQNLIKAMVDPDPARRPSAREILQNPIFDKVRNHPK 489
            + NL++GK+P+ P  S  F  LI  M+ P P +RP+++ I  +PI   V +  K
Sbjct: 472 EWHNLRDGKVPILPSLSRDFNELIAQMMHPYPDKRPTSQSIFSHPILSAVDSKSK 525

[Close]

BLAST of CmaCh08G004070.1 vs. Swiss-Prot
Match: WEE1A_XENLA (Wee1-like protein kinase 1-A OS=Xenopus laevis GN=wee1-a PE=2 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 3.0e-47
Identity = 100/277 (36.10%), Postives = 159/277 (57.40%), Query Frame = 1


Query: 232 SRYHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRSLHQDIERRRALMEVQALAA 291
           SRY T+FHE+++IG+G F  VFK +KR+DGC+YA+K+S + +   ++ + AL EV A A 
Sbjct: 218 SRYATEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPMAGSVDEQNALREVYAHAV 277

Query: 292 LGSHENIVGYYSSWFENEQLYIQMELC------DCSLSTGRYSHPFSEADALKALHQIAK 351
           LG H ++V YYS+W E++ + IQ E C      D      R    F+E +    L Q+A+
Sbjct: 278 LGQHPHVVRYYSAWAEDDHMLIQNEYCNGGSLADAISENYRTMQYFTEPELKDLLLQVAR 337

Query: 352 ALLFMHEKGVAHLDVKPDNIY----------VKDG----------VYKLGDFGCATLLDK 411
            L ++H   + H+D+KP NI+          V++G          VYK+GD G  T +  
Sbjct: 338 GLKYIHSMSLVHMDIKPSNIFISRITVPNTGVEEGDDEDCGSGNVVYKIGDLGHVTRV-S 397

Query: 412 SLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEVVRGSTFPET--RFMNLKEGKL 471
           S  +EEGD+R++  E+L E Y +L K DIF+L   ++       FP    ++  +++GKL
Sbjct: 398 SPQVEEGDSRFLANEVLQEDYTHLAKADIFALALTVWCAAGAEPFPTNGDQWHEIRQGKL 457

Query: 472 PLFP-CHSLQFQNLIKAMVDPDPARRPSAREILQNPI 480
           P  P   S +F +LIK M+ PD  +RPS+  ++++ +
Sbjct: 458 PRVPQLLSQEFVDLIKLMISPDSEKRPSSMALVKHSV 493

[Close]

BLAST of CmaCh08G004070.1 vs. TrEMBL
Match: A0A0A0K5B3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G395770 PE=3 SV=1)

HSP 1 Score: 867.5 bits (2240), Expect = 8.0e-249
Identity = 426/489 (87.12%), Postives = 449/489 (91.82%), Query Frame = 1


Query: 1   MRRKSKTLRTTMTATRKKMKGTLTRHLQVQLGQVSLAPLHHQLSSSTAACFQDLPESEAM 60
           MRRKSKTLRTTM A RKKMKGT+TRHLQVQLG+VSLAPLH   SSS+A CF  LPESE  
Sbjct: 1   MRRKSKTLRTTMGARRKKMKGTVTRHLQVQLGKVSLAPLHLNRSSSSALCFPSLPESEPP 60

Query: 61  ATVNDSGALPSSSTNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDCYKESTPCPKSPEK 120
           +TVN S  LPSSS NFEDRDFILSQDFFCTPDYITPDNQNLLNGVD  KE  PCPKSPEK
Sbjct: 61  STVNFSDPLPSSSHNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCPKSPEK 120

Query: 121 INTVKTKRKIRDGVLVHPLSPSLSGREQVVELGNDTFGTDDEDMEKETMAMPKKQNYVSQ 180
           +NTVKTKRKI + VLV PLSPSLSG EQVVELGND FG+DD DMEKET AMPKK+NYVSQ
Sbjct: 121 VNTVKTKRKIHENVLVRPLSPSLSGCEQVVELGNDIFGSDDVDMEKETTAMPKKENYVSQ 180

Query: 181 SAVALRCRVMPPPCMKNPYLNDASDIGVDPFGNQRTKCADFFPALFSGDGLSRYHTDFHE 240
           SAVALRCRVMPPPCMKNPYL DASD+GVDPFGNQRTKCA FFPALFSGDGLSRYHTDFHE
Sbjct: 181 SAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHE 240

Query: 241 IKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRSLHQDIERRRALMEVQALAALGSHENIVG 300
           IK IGTGNFSRVFKVLKRIDGCLYAVKQSTR L+QD ERRRALMEVQALAALG+HENIVG
Sbjct: 241 IKLIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGAHENIVG 300

Query: 301 YYSSWFENEQLYIQMELCDCSLSTGRYSHPFSEADALKALHQIAKALLFMHEKGVAHLDV 360
           YY+SWFENEQLYIQMELCDCSLS GRYSHPFSE DAL+AL+QIAKALLF+HEKG+AHLDV
Sbjct: 301 YYTSWFENEQLYIQMELCDCSLSMGRYSHPFSEVDALRALYQIAKALLFVHEKGIAHLDV 360

Query: 361 KPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA 420
           KPDNIY+KDGVYKLGDFGC TLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA
Sbjct: 361 KPDNIYIKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA 420

Query: 421 IYEVVRGSTFPETRFMNLKEGKLPLFPCHSLQFQNLIKAMVDPDPARRPSAREILQNPIF 480
           IYE+VRG T PET FMNLKEGKLPL P HSLQFQNLIKAMVDPDP+RRPSARE+++NPIF
Sbjct: 421 IYEIVRGFTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPSRRPSAREVIENPIF 480

Query: 481 DKVRNHPKS 490
           DKVR H +S
Sbjct: 481 DKVRTHKQS 489

[Close]

BLAST of CmaCh08G004070.1 vs. TrEMBL
Match: A0A0D2SJ55_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_007G129700 PE=3 SV=1)

HSP 1 Score: 658.7 bits (1698), Expect = 5.6e-186
Identity = 323/479 (67.43%), Postives = 385/479 (80.38%), Query Frame = 1


Query: 18  KMKGTLTRHLQVQLGQVSLAPLHHQLSSSTAACFQDLPESEAMATVNDSGALPSSSTNF- 77
           KMKGTL +H QVQ         HHQ SS+T++       S     + D G+    ST+  
Sbjct: 12  KMKGTLGKHFQVQFH-------HHQSSSATSS-------SIFTRNLLDFGSQDDPSTSVP 71

Query: 78  -----EDRDFILSQDFFCTPDYITPDNQNLLNGVDCYKESTPCPKSPEKINTVKTKRKIR 137
                +D+DFILSQDFFCTPDYITPDNQNL+NG DC KE+ PCPKSPEK+NT+K+KR  +
Sbjct: 72  DLDAADDKDFILSQDFFCTPDYITPDNQNLMNGFDCNKENIPCPKSPEKLNTLKSKRPRQ 131

Query: 138 DGVLVHPLSPSLSGREQVVELGNDTFGTDDEDMEKETM-AMPKKQNYVSQSAVALRCRVM 197
           DG+LV+PLSP+LSG +Q+VEL +D F  D+ ++EK  +  + K +NYVSQSAVALRCRVM
Sbjct: 132 DGILVNPLSPTLSGSQQIVELASDNFDADEGNLEKAIIPGVQKTKNYVSQSAVALRCRVM 191

Query: 198 PPPCMKNPYLNDASDIGVDPFGNQRTKCADFFPALFSGDGLSRYHTDFHEIKQIGTGNFS 257
           PPPC+KNPYL DAS++  DPFGNQR+KCA FFPA+  GDGLSRYHTDFHEI+QIGTG+FS
Sbjct: 192 PPPCIKNPYLKDASEVDTDPFGNQRSKCAGFFPAIIGGDGLSRYHTDFHEIEQIGTGHFS 251

Query: 258 RVFKVLKRIDGCLYAVKQSTRSLHQDIERRRALMEVQALAALGSHENIVGYYSSWFENEQ 317
           RVFKVLKRIDGCLYAVK STR LHQ+ ERR+ALMEVQALAALGSHEN VGYYSSWFENEQ
Sbjct: 252 RVFKVLKRIDGCLYAVKHSTRPLHQETERRKALMEVQALAALGSHENTVGYYSSWFENEQ 311

Query: 318 LYIQMELCDCSLSTGRYSHPFSEADALKALHQIAKALLFMHEKGVAHLDVKPDNIYVKDG 377
           LYIQMELCD SLS    +  F+E + L+A+HQ+AKAL F+HEKG+ HLDVKPDN+YVK+G
Sbjct: 312 LYIQMELCDYSLSRKSSTQLFTEGEFLRAMHQMAKALQFIHEKGIVHLDVKPDNVYVKNG 371

Query: 378 VYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEVVRGSTF 437
           VYKLGDFGCATLL+K+LP+EEGDARYMPQEILNE YD+LDKVDIFSLG  IYE++RGS  
Sbjct: 372 VYKLGDFGCATLLNKNLPVEEGDARYMPQEILNENYDHLDKVDIFSLGVTIYELIRGSPL 431

Query: 438 PETRFMNLKEGKLPLFPCHSLQFQNLIKAMVDPDPARRPSAREILQNPIFDKVRNHPKS 490
           PE+    L+EGKLPL   +SLQFQNL+K MVD DP RRPSA+E+++NPIFDK   + K+
Sbjct: 432 PESGPQFLREGKLPLLAGYSLQFQNLLKVMVDHDPVRRPSAKELVENPIFDKASKNTKA 476

[Close]

BLAST of CmaCh08G004070.1 vs. TrEMBL
Match: A0A061DY89_THECC (Kinase WEE1 isoform 1 OS=Theobroma cacao GN=TCM_004583 PE=3 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 9.0e-184
Identity = 329/492 (66.87%), Postives = 386/492 (78.46%), Query Frame = 1


Query: 1   MRRKSKTLRTTMTATRKKMKGTLTRHLQVQLGQVSLAPLHHQLSSSTAACF--QDLPESE 60
           M+RK     T       KMKGTL R LQVQL        HHQ SS+ ++    ++L +SE
Sbjct: 1   MKRKVVKRGTNRRRESGKMKGTLGRLLQVQLH-------HHQSSSANSSSLFARNLLDSE 60

Query: 61  AMATVNDSGALPSSSTNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDCYKESTPCPKSP 120
                  + AL   +   +D+DFILSQDFFCTPDYITPDNQNL+NG DC K++ PCPKSP
Sbjct: 61  PDDPSTSASALDLDAA--DDKDFILSQDFFCTPDYITPDNQNLMNGFDCNKDNIPCPKSP 120

Query: 121 EKINTVKTKRKIRDGVLVHPLSPSLSGREQVVELGNDTFGTDDEDMEKETM-AMPKKQNY 180
           EK++T+K+KR  +DG+ V+PLSP LSG +Q+VEL  D F  D  ++EK  +  + K +NY
Sbjct: 121 EKLHTLKSKRPRQDGIQVNPLSPILSGSQQIVELAKDNFDVDVVNLEKTVIPGVQKTKNY 180

Query: 181 VSQSAVALRCRVMPPPCMKNPYLNDASDIGVDPFGNQRTKCADFFPALFSGDGLSRYHTD 240
           VSQSAVALRCRVMPPPC+KNPYL DAS++  DP GNQR+KCA FFPA+  GDGLSRYHTD
Sbjct: 181 VSQSAVALRCRVMPPPCIKNPYLKDASEMDTDPLGNQRSKCAGFFPAIIGGDGLSRYHTD 240

Query: 241 FHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRSLHQDIERRRALMEVQALAALGSHEN 300
           FHEI+QIGTGNFSRVFKVLKRIDGCLYAVK +TR LHQD ERR+ALMEVQALAALGSHEN
Sbjct: 241 FHEIEQIGTGNFSRVFKVLKRIDGCLYAVKHTTRQLHQDTERRKALMEVQALAALGSHEN 300

Query: 301 IVGYYSSWFENEQLYIQMELCDCSLSTGRYSHPFSEADALKALHQIAKALLFMHEKGVAH 360
           IVGYYSSWFENEQLYIQMELCD SLS       F+E + L+A  QIAKAL  +HE+G+AH
Sbjct: 301 IVGYYSSWFENEQLYIQMELCDYSLSRNTSLQLFTEGEVLRAWLQIAKALQCIHERGIAH 360

Query: 361 LDVKPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSL 420
           LDVKPDNIYVK+G YKLGDFGCATLL+KSLPIEEGDARYMPQEILNE YD+LDKVDIFSL
Sbjct: 361 LDVKPDNIYVKNGAYKLGDFGCATLLNKSLPIEEGDARYMPQEILNENYDHLDKVDIFSL 420

Query: 421 GAAIYEVVRGSTFPETRFMNLKEGKLPLFPCHSLQFQNLIKAMVDPDPARRPSAREILQN 480
           G  IYE++RGS   E+    L+EGKLPL P HS+QFQNL+K MVD DP RRPSA+E+++N
Sbjct: 421 GVTIYELIRGSPLQESGRQFLREGKLPLLPGHSIQFQNLLKVMVDQDPVRRPSAKELIEN 480

Query: 481 PIFDKVRNHPKS 490
           PIFDKV N+ K+
Sbjct: 481 PIFDKVSNNAKA 483

[Close]

BLAST of CmaCh08G004070.1 vs. TrEMBL
Match: M5VNA9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa015605mg PE=3 SV=1)

HSP 1 Score: 645.6 bits (1664), Expect = 4.9e-182
Identity = 323/475 (68.00%), Postives = 382/475 (80.42%), Query Frame = 1


Query: 19  MKGTLTRHLQVQLGQVSLAPLHHQLSSSTAACFQDLPESEAMATVNDSGALPSSST---- 78
           MKG L R    +  Q+S  P   +LSSS ++ FQ+L +S A   +     LPSSS     
Sbjct: 1   MKGALARLQLGESHQLSFPP-SSKLSSSNSSRFQNLLDSGAADAI-----LPSSSPLGTD 60

Query: 79  ---NFEDRDFILSQDFFCTPDYITPDNQNLLNGVDCYKESTPCPKSPEKINTVKTKRKIR 138
              NF+DRDFILSQDFFCTPDYITPDNQ+ LNG DC KE+ PCPKSPEK NTVK+KR+ +
Sbjct: 61  LDPNFDDRDFILSQDFFCTPDYITPDNQHTLNGWDCNKENIPCPKSPEKSNTVKSKRRRQ 120

Query: 139 DGVLVHPLSPSLSGREQVVELGNDTFGTDDEDMEKETMAMPKK-QNYVSQSAVALRCRVM 198
             VL++PLSP+ SG +Q+VELG D+FG D+  +EK T+   +K QNYVSQSAVALRCRVM
Sbjct: 121 GDVLLNPLSPTFSGNQQIVELGTDSFGIDEAKIEKPTVTEVRKTQNYVSQSAVALRCRVM 180

Query: 199 PPPCMKNPYLNDASDIGVDPFGNQRTKCADFFPALFSGDGLSRYHTDFHEIKQIGTGNFS 258
           PPPC KNPYL DAS++ +DP GNQR+KCA FFPA+  G+GLSRY  DFHEI+QIG+G FS
Sbjct: 181 PPPCFKNPYLKDASEMDIDPLGNQRSKCAAFFPAIMGGNGLSRYCADFHEIEQIGSGYFS 240

Query: 259 RVFKVLKRIDGCLYAVKQSTRSLHQDIERRRALMEVQALAALGSHENIVGYYSSWFENEQ 318
            VFK L RIDGCLYAVK S + LHQD ERR+ALMEVQ+LAALGSHENIVGYYSSWFENEQ
Sbjct: 241 HVFKALNRIDGCLYAVKLSMKQLHQDTERRKALMEVQSLAALGSHENIVGYYSSWFENEQ 300

Query: 319 LYIQMELCDCSLSTGRYSHPFSEADALKALHQIAKALLFMHEKGVAHLDVKPDNIYVKDG 378
           LYIQMELCD SLS  ++S  F+E + L+ L QIAKAL F+H+KG+AHLDVKPDNIYVK+G
Sbjct: 301 LYIQMELCDHSLSIYKFSRTFTEGEILEVLFQIAKALQFIHKKGIAHLDVKPDNIYVKNG 360

Query: 379 VYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEVVRGSTF 438
           VYKLGDFGCATLLDKSLPIEEGDARYMPQEILNE+YD LDK DIFSLG AIYE++RG   
Sbjct: 361 VYKLGDFGCATLLDKSLPIEEGDARYMPQEILNEKYDDLDKADIFSLGVAIYELIRGLPL 420

Query: 439 PET--RFMNLKEGKLPLFPCHSLQFQNLIKAMVDPDPARRPSAREILQNPIFDKV 484
           PE+  + +NL+EGKLPL P HSLQFQNL+K M+DP+P  RPSA+++++NPIFDKV
Sbjct: 421 PESGPQILNLREGKLPLLPGHSLQFQNLLKVMLDPNPVWRPSAKDLVENPIFDKV 469

[Close]

BLAST of CmaCh08G004070.1 vs. TrEMBL
Match: D7TP05_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_07s0104g01740 PE=3 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 2.4e-181
Identity = 320/474 (67.51%), Postives = 384/474 (81.01%), Query Frame = 1


Query: 19  MKGTLTRHLQVQLGQVSLAPLHHQLSSSTAACFQDLPESEAMATVNDSGALPSSSTNFED 78
           MKGTL RHL VQLGQVS                Q+L ESE+  TV++  A      + +D
Sbjct: 1   MKGTLARHLTVQLGQVSRC--------------QNLLESES--TVDNIDA------DVDD 60

Query: 79  RDFILSQDFFCTPDYITPDNQNLLNGVDCYKESTPCPKSPEKINTVKTKRKIRDGVLVHP 138
           +DFILSQDFFCTPDYITPD QN+LNG+DC K++ PCPKSPEK+ T + KRK  D  L+  
Sbjct: 61  KDFILSQDFFCTPDYITPDGQNILNGLDCNKDNIPCPKSPEKLKTSRIKRKREDDTLLDS 120

Query: 139 LSPSLSGREQVVELGNDTFGTDDEDMEKETMA-MPKKQNYVSQSAVALRCRVMPPPCMKN 198
            + +L   + ++EL  DTFGTD+  +EK  +    K Q+YVSQSAVALRCRVMPPPC+KN
Sbjct: 121 PNSTLFD-QPILELEKDTFGTDEVKVEKAIVTGSQKNQSYVSQSAVALRCRVMPPPCLKN 180

Query: 199 PYLNDASDIGVDPFGNQRTKCADFFPALFSGDGLSRYHTDFHEIKQIGTGNFSRVFKVLK 258
           PYL +AS+  +DPFGN+R+K + FFPAL  GDGLSRYHTDFHEI+QIG GNFSRVFKVLK
Sbjct: 181 PYLKNASETDIDPFGNRRSKYSGFFPALIGGDGLSRYHTDFHEIEQIGDGNFSRVFKVLK 240

Query: 259 RIDGCLYAVKQSTRSLHQDIERRRALMEVQALAALGSHENIVGYYSSWFENEQLYIQMEL 318
           RIDGC+YAVK STR LHQD ERR+ALMEVQALA LGSHENIVGYY+SWFENE+LYIQMEL
Sbjct: 241 RIDGCMYAVKHSTRPLHQDTERRKALMEVQALAVLGSHENIVGYYTSWFENEKLYIQMEL 300

Query: 319 CDCSLSTGRYSHPFSEADALKALHQIAKALLFMHEKGVAHLDVKPDNIYVKDGVYKLGDF 378
           CD SLS  R SH F+E + L+A+HQIAKAL F HEKG+AHLDVKPDNIYVKDGVYKLGDF
Sbjct: 301 CDHSLSIKRSSHLFTEGEVLEAMHQIAKALQFTHEKGIAHLDVKPDNIYVKDGVYKLGDF 360

Query: 379 GCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEVVRGSTFPET--RF 438
           GCATLLD+SLPI+EGDARYMPQEILN+ YD+LDKVD+FSLGAAIYE++RGS  PE+  +F
Sbjct: 361 GCATLLDRSLPIDEGDARYMPQEILNDNYDHLDKVDMFSLGAAIYELIRGSHLPESGYQF 420

Query: 439 MNLKEGKLPLFPCHSLQFQNLIKAMVDPDPARRPSAREILQNPIFDKVRNHPKS 490
           +NL+EGKLPL P +SLQFQNL+KAM+DPDP +RPSA+E+++NPIFD+++ + K+
Sbjct: 421 LNLREGKLPLLPGYSLQFQNLLKAMLDPDPVQRPSAKELVENPIFDRLQRNAKT 451

[Close]

BLAST of CmaCh08G004070.1 vs. TAIR10
Match: AT1G02970.1 (AT1G02970.1 WEE1 kinase homolog)

HSP 1 Score: 579.3 bits (1492), Expect = 2.2e-165
Identity = 293/488 (60.04%), Postives = 369/488 (75.61%), Query Frame = 1


Query: 2   RRKSKTLRTTMTATRKKMKGTLTRHLQVQLGQVSLAPLHHQLSSSTAA-CFQDLPESEAM 61
           R+   T++T  +   +KM+GTL RH  +Q GQ+S     ++ SS+ A+  FQ L +S++ 
Sbjct: 13  RKTQGTIKTRASKKIRKMEGTLERHSLLQFGQLSKISFENRPSSNVASSAFQGLLDSDSS 72

Query: 62  ATVNDSGALPSSSTNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDCYKESTPCPKSPEK 121
              N  G+  S + N  ++DFILSQDFFCTPDYITPDNQNL++G+D  K+ +PCP+SP K
Sbjct: 73  ELRNQLGSADSDA-NCGEKDFILSQDFFCTPDYITPDNQNLMSGLDISKDHSPCPRSPVK 132

Query: 122 INTVKTKRKIRDGVLVHPLSPSLSGREQVVELGNDTFGTDDEDMEKETMAMPKKQNYVSQ 181
           +NTVK+KR  ++    +  + + S + +V E  ND   TD+   +K      ++  YVSQ
Sbjct: 133 LNTVKSKRCRQESFTGNHSNSTWSSKHRVDEQENDDIDTDEVMGDKLQANQTERTGYVSQ 192

Query: 182 SAVALRCRVMPPPCMKNPYLNDASDIGVDPFGNQRTKCADFFPALFSGDGLSRYHTDFHE 241
           +AVALRCR MPPPC+KNPY+ + S+   DPFG+QR+KCA F P   SGDGLSRY TDFHE
Sbjct: 193 AAVALRCRAMPPPCLKNPYVLNQSETATDPFGHQRSKCASFLPVSTSGDGLSRYLTDFHE 252

Query: 242 IKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRSLHQDIERRRALMEVQALAALGSHENIVG 301
           I+QIG G+FSRVFKVLKR+DGCLYAVK STR L+ D ERR+A+MEVQALAALG HENIVG
Sbjct: 253 IRQIGAGHFSRVFKVLKRMDGCLYAVKHSTRKLYLDSERRKAMMEVQALAALGFHENIVG 312

Query: 302 YYSSWFENEQLYIQMELCDCSLST--GRYSHPFSEADALKALHQIAKALLFMHEKGVAHL 361
           YYSSWFENEQLYIQ+ELCD SLS    + S   SE + L  +HQIAKAL F+HEKG+AHL
Sbjct: 313 YYSSWFENEQLYIQLELCDHSLSALPKKSSLKVSEREILVIMHQIAKALHFVHEKGIAHL 372

Query: 362 DVKPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLG 421
           DVKPDNIY+K+GV KLGDFGCAT LDKSLP+EEGDARYMPQEILNE Y++LDKVDIFSLG
Sbjct: 373 DVKPDNIYIKNGVCKLGDFGCATRLDKSLPVEEGDARYMPQEILNEDYEHLDKVDIFSLG 432

Query: 422 AAIYEVVRGSTFPETR--FMNLKEGKLPLFPCHSLQFQNLIKAMVDPDPARRPSAREILQ 481
             +YE+++GS   E+R   +N+KEGKLPL P HSLQ Q L+K M+D DP RRPSARE+L 
Sbjct: 433 VTVYELIKGSPLTESRNQSLNIKEGKLPLLPGHSLQLQQLLKTMMDRDPKRRPSARELLD 492

Query: 482 NPIFDKVR 485
           +P+FD++R
Sbjct: 493 HPMFDRIR 499

[Close]

BLAST of CmaCh08G004070.1 vs. TAIR10
Match: AT4G19110.2 (AT4G19110.2 Protein kinase superfamily protein)

HSP 1 Score: 96.7 bits (239), Expect = 4.3e-20
Identity = 59/189 (31.22%), Postives = 99/189 (52.38%), Query Frame = 1


Query: 241 IKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRSLHQDIERRRALMEVQALAALGSHENIVG 300
           IK++G G F  V++ + +  G + A+K+  +  +   E    L EV++L  + +H NIV 
Sbjct: 7   IKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYSWDECIN-LREVKSLRRM-NHPNIVK 66

Query: 301 YYSSWFENEQLYIQMELCDCSLSTGRYSHP--FSEADALKALHQIAKALLFMHEKGVAHL 360
                 EN+ LY   E  +C+L          F+EAD      Q+ + L +MH++G  H 
Sbjct: 67  LKEVIRENDILYFVFEYMECNLYQLMKDRQKLFAEADIKNWCFQVFQGLSYMHQRGYFHR 126

Query: 361 DVKPDNIYVKDGVYKLGDFGCATLLDKSLPIEE--GDARYMPQEILNERYDYLDKVDIFS 420
           D+KP+N+ V   + K+ DFG A  ++ S P  E      Y   E+L + Y Y  KVD+++
Sbjct: 127 DLKPENLLVSKDIIKIADFGLAREVNSSPPFTEYVSTRWYRAPEVLLQSYVYTSKVDMWA 186

Query: 421 LGAAIYEVV 426
           +GA + E++
Sbjct: 187 MGAIMAELL 193

[Close]

BLAST of CmaCh08G004070.1 vs. TAIR10
Match: AT3G20860.1 (AT3G20860.1 NIMA-related kinase 5)

HSP 1 Score: 96.3 bits (238), Expect = 5.6e-20
Identity = 77/259 (29.73%), Postives = 123/259 (47.49%), Query Frame = 1


Query: 237 DFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRSLHQDIERRRALMEVQALAALGSHE 296
           D+  ++QIG G F   F V+ + +   Y VK+   +   +  +  A+ E+  ++ L S  
Sbjct: 14  DYEVVEQIGRGAFGSAFLVIHKSERRKYVVKKIRLAKQTERCKLAAIQEMSLISKLKS-P 73

Query: 297 NIVGYYSSWFENEQLYIQMELCD-------CSLSTGRYSHPFSEADALKALHQIAKALLF 356
            IV Y  SW E + + I    C+          S G ++   SE    + + Q+  A+ +
Sbjct: 74  YIVEYKDSWVEKDCVCIVTSYCEGGDMTQMIKKSRGVFA---SEEKLCRWMVQLLLAIDY 133

Query: 357 MHEKGVAHLDVKPDNIYV-KDGVYKLGDFGCATLLDKSLPIEE--GDARYMPQEILNE-R 416
           +H   V H D+K  NI++ K+   +LGDFG A LL K        G   YM  E+L +  
Sbjct: 134 LHNNRVLHRDLKCSNIFLTKENEVRLGDFGLAKLLGKDDLASSMVGTPNYMCPELLADIP 193

Query: 417 YDYLDKVDIFSLGAAIYEVVRGSTFPETRFMNLKEGKL------PLFPCHSLQFQNLIKA 476
           Y Y  K DI+SLG  ++EV       +   M     K+      PL   +S   + LIK+
Sbjct: 194 YGY--KSDIWSLGCCMFEVAAHQPAFKAPDMAALINKINRSSLSPLPVMYSSSLKRLIKS 253

Query: 477 MVDPDPARRPSAREILQNP 479
           M+  +P  RP+A E+L++P
Sbjct: 254 MLRKNPEHRPTAAELLRHP 266

[Close]

BLAST of CmaCh08G004070.1 vs. TAIR10
Match: AT5G45430.1 (AT5G45430.1 Protein kinase superfamily protein)

HSP 1 Score: 96.3 bits (238), Expect = 5.6e-20
Identity = 58/189 (30.69%), Postives = 99/189 (52.38%), Query Frame = 1


Query: 241 IKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRSLHQDIERRRALMEVQALAALGSHENIVG 300
           +K++G G F  V++ + +    + A+K+  +  +   E    L EV++L+ + +H NIV 
Sbjct: 7   LKEVGDGTFGNVWRAVNKQTNEVVAIKRMKKK-YFSWEECVNLREVKSLSRM-NHPNIVK 66

Query: 301 YYSSWFENEQLYIQMELCDCSLSTGRYSHP--FSEADALKALHQIAKALLFMHEKGVAHL 360
                 EN+ LY   E  +C+L       P  F+E+D      Q+ + L +MH++G  H 
Sbjct: 67  LKEVIRENDILYFVFEYMECNLYQLMKDRPKHFAESDIRNWCFQVFQGLSYMHQRGYFHR 126

Query: 361 DVKPDNIYVKDGVYKLGDFGCATLLDKSLPIEE--GDARYMPQEILNERYDYLDKVDIFS 420
           D+KP+N+ V   V K+ D G A  +D S P  E      Y   E+L + Y Y  KVD+++
Sbjct: 127 DLKPENLLVSKDVIKIADLGLAREIDSSPPYTEYVSTRWYRAPEVLLQSYVYTSKVDMWA 186

Query: 421 LGAAIYEVV 426
           +GA + E++
Sbjct: 187 MGAIMAELL 193

[Close]

BLAST of CmaCh08G004070.1 vs. TAIR10
Match: AT1G18890.1 (AT1G18890.1 calcium-dependent protein kinase 1)

HSP 1 Score: 94.7 bits (234), Expect = 1.6e-19
Identity = 73/268 (27.24%), Postives = 120/268 (44.78%), Query Frame = 1


Query: 242 KQIGTGNFSRVFKVLKRIDGCLYAVKQ-STRSLHQDIERRRALMEVQALAALGSHENIVG 301
           +++G G F   +    R      A K  S R L   ++      EV  ++ L  H N+V 
Sbjct: 67  RELGRGEFGITYLCTDRETHEALACKSISKRKLRTAVDIEDVRREVAIMSTLPEHPNVVK 126

Query: 302 YYSSWFENEQLYIQMELC------DCSLSTGRYSHPFSEADALKALHQIAKALLFMHEKG 361
             +S+ +NE +++ MELC      D  ++ G Y    +E  A      IA+ ++  H  G
Sbjct: 127 LKASYEDNENVHLVMELCEGGELFDRIVARGHY----TERAAAAVARTIAEVVMMCHSNG 186

Query: 362 VAHLDVKPDNIYV----KDGVYKLGDFGCATLLDKSLPIEE--GDARYMPQEILNERYDY 421
           V H D+KP+N       ++   K  DFG +          E  G   YM  E+L  + DY
Sbjct: 187 VMHRDLKPENFLFANKKENSPLKAIDFGLSVFFKPGDKFTEIVGSPYYMAPEVL--KRDY 246

Query: 422 LDKVDIFSLGAAIYEVVRG--STFPETR-------FMNLKEGKLPLFPCHSLQFQNLIKA 481
              VD++S G  IY ++ G    + ET           + + K   +P  S   ++L+K 
Sbjct: 247 GPGVDVWSAGVIIYILLCGVPPFWAETEQGVALAILRGVLDFKRDPWPQISESAKSLVKQ 306

Query: 482 MVDPDPARRPSAREILQNPIFDKVRNHP 488
           M+DPDP +R +A+++L +P     +  P
Sbjct: 307 MLDPDPTKRLTAQQVLAHPWIQNAKKAP 328

[Close]

BLAST of CmaCh08G004070.1 vs. NCBI nr
Match: gi|659101452|ref|XP_008451611.1| (PREDICTED: wee1-like protein kinase [Cucumis melo])

HSP 1 Score: 872.8 bits (2254), Expect = 2.7e-250
Identity = 430/489 (87.93%), Postives = 453/489 (92.64%), Query Frame = 1


Query: 1   MRRKSKTLRTTMTATRKKMKGTLTRHLQVQLGQVSLAPLHHQLSSSTAACFQDLPESEAM 60
           MRRKSKTLRTTM   RKKMKGT+TRHLQVQLG+VSLAPL    SSS+AACFQ+LPESEA 
Sbjct: 1   MRRKSKTLRTTMGTRRKKMKGTVTRHLQVQLGKVSLAPLQLHRSSSSAACFQNLPESEAP 60

Query: 61  ATVNDSGALPSSSTNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDCYKESTPCPKSPEK 120
           +TVN S ALPSSS+NFEDRDFILSQDFFCTPDYITPDNQNLLNGVD  KE  PCPKSPEK
Sbjct: 61  STVNVSDALPSSSSNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCPKSPEK 120

Query: 121 INTVKTKRKIRDGVLVHPLSPSLSGREQVVELGNDTFGTDDEDMEKETMAMPKKQNYVSQ 180
           INTVKTKRKI + VLV PLSPSLSG EQVVELGNDTFG+DD DMEKETMAMPKK++YVSQ
Sbjct: 121 INTVKTKRKIHENVLVRPLSPSLSGCEQVVELGNDTFGSDDVDMEKETMAMPKKESYVSQ 180

Query: 181 SAVALRCRVMPPPCMKNPYLNDASDIGVDPFGNQRTKCADFFPALFSGDGLSRYHTDFHE 240
           SAVALRCRVMPPPCMKNPYL DASD+GVDPFGNQRTKCA FFPALFSGDGLSRYHTDFHE
Sbjct: 181 SAVALRCRVMPPPCMKNPYLGDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHE 240

Query: 241 IKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRSLHQDIERRRALMEVQALAALGSHENIVG 300
           IK IGTGNFSRVFKVLKRIDGCLYAVKQSTR L+QD ERRRALMEVQALAALGSHENIVG
Sbjct: 241 IKLIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGSHENIVG 300

Query: 301 YYSSWFENEQLYIQMELCDCSLSTGRYSHPFSEADALKALHQIAKALLFMHEKGVAHLDV 360
           YY+SWFENEQLYIQMELCDCSLS G YSHPFSE DAL+AL+QIAKALLF+HEKG+AHLDV
Sbjct: 301 YYTSWFENEQLYIQMELCDCSLSMGTYSHPFSEVDALRALYQIAKALLFVHEKGIAHLDV 360

Query: 361 KPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA 420
           KPDNIY+KDGVYKLGDFGC TLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA
Sbjct: 361 KPDNIYIKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA 420

Query: 421 IYEVVRGSTFPETRFMNLKEGKLPLFPCHSLQFQNLIKAMVDPDPARRPSAREILQNPIF 480
           IYE+VRG T PET FMNLKEGKLPL P HSLQFQNLIKAMVDP+P RRPSARE+L+NPIF
Sbjct: 421 IYEIVRGFTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPNPTRRPSAREVLENPIF 480

Query: 481 DKVRNHPKS 490
           DKVR H +S
Sbjct: 481 DKVRTHKQS 489

[Close]

BLAST of CmaCh08G004070.1 vs. NCBI nr
Match: gi|449436531|ref|XP_004136046.1| (PREDICTED: wee1-like protein kinase [Cucumis sativus])

HSP 1 Score: 867.5 bits (2240), Expect = 1.1e-248
Identity = 426/489 (87.12%), Postives = 449/489 (91.82%), Query Frame = 1


Query: 1   MRRKSKTLRTTMTATRKKMKGTLTRHLQVQLGQVSLAPLHHQLSSSTAACFQDLPESEAM 60
           MRRKSKTLRTTM A RKKMKGT+TRHLQVQLG+VSLAPLH   SSS+A CF  LPESE  
Sbjct: 1   MRRKSKTLRTTMGARRKKMKGTVTRHLQVQLGKVSLAPLHLNRSSSSALCFPSLPESEPP 60

Query: 61  ATVNDSGALPSSSTNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDCYKESTPCPKSPEK 120
           +TVN S  LPSSS NFEDRDFILSQDFFCTPDYITPDNQNLLNGVD  KE  PCPKSPEK
Sbjct: 61  STVNFSDPLPSSSHNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCPKSPEK 120

Query: 121 INTVKTKRKIRDGVLVHPLSPSLSGREQVVELGNDTFGTDDEDMEKETMAMPKKQNYVSQ 180
           +NTVKTKRKI + VLV PLSPSLSG EQVVELGND FG+DD DMEKET AMPKK+NYVSQ
Sbjct: 121 VNTVKTKRKIHENVLVRPLSPSLSGCEQVVELGNDIFGSDDVDMEKETTAMPKKENYVSQ 180

Query: 181 SAVALRCRVMPPPCMKNPYLNDASDIGVDPFGNQRTKCADFFPALFSGDGLSRYHTDFHE 240
           SAVALRCRVMPPPCMKNPYL DASD+GVDPFGNQRTKCA FFPALFSGDGLSRYHTDFHE
Sbjct: 181 SAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGFFPALFSGDGLSRYHTDFHE 240

Query: 241 IKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRSLHQDIERRRALMEVQALAALGSHENIVG 300
           IK IGTGNFSRVFKVLKRIDGCLYAVKQSTR L+QD ERRRALMEVQALAALG+HENIVG
Sbjct: 241 IKLIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGAHENIVG 300

Query: 301 YYSSWFENEQLYIQMELCDCSLSTGRYSHPFSEADALKALHQIAKALLFMHEKGVAHLDV 360
           YY+SWFENEQLYIQMELCDCSLS GRYSHPFSE DAL+AL+QIAKALLF+HEKG+AHLDV
Sbjct: 301 YYTSWFENEQLYIQMELCDCSLSMGRYSHPFSEVDALRALYQIAKALLFVHEKGIAHLDV 360

Query: 361 KPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA 420
           KPDNIY+KDGVYKLGDFGC TLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA
Sbjct: 361 KPDNIYIKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAA 420

Query: 421 IYEVVRGSTFPETRFMNLKEGKLPLFPCHSLQFQNLIKAMVDPDPARRPSAREILQNPIF 480
           IYE+VRG T PET FMNLKEGKLPL P HSLQFQNLIKAMVDPDP+RRPSARE+++NPIF
Sbjct: 421 IYEIVRGFTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPSRRPSAREVIENPIF 480

Query: 481 DKVRNHPKS 490
           DKVR H +S
Sbjct: 481 DKVRTHKQS 489

[Close]

BLAST of CmaCh08G004070.1 vs. NCBI nr
Match: gi|823188157|ref|XP_012490421.1| (PREDICTED: wee1-like protein kinase [Gossypium raimondii])

HSP 1 Score: 658.7 bits (1698), Expect = 8.1e-186
Identity = 323/479 (67.43%), Postives = 385/479 (80.38%), Query Frame = 1


Query: 18  KMKGTLTRHLQVQLGQVSLAPLHHQLSSSTAACFQDLPESEAMATVNDSGALPSSSTNF- 77
           KMKGTL +H QVQ         HHQ SS+T++       S     + D G+    ST+  
Sbjct: 12  KMKGTLGKHFQVQFH-------HHQSSSATSS-------SIFTRNLLDFGSQDDPSTSVP 71

Query: 78  -----EDRDFILSQDFFCTPDYITPDNQNLLNGVDCYKESTPCPKSPEKINTVKTKRKIR 137
                +D+DFILSQDFFCTPDYITPDNQNL+NG DC KE+ PCPKSPEK+NT+K+KR  +
Sbjct: 72  DLDAADDKDFILSQDFFCTPDYITPDNQNLMNGFDCNKENIPCPKSPEKLNTLKSKRPRQ 131

Query: 138 DGVLVHPLSPSLSGREQVVELGNDTFGTDDEDMEKETM-AMPKKQNYVSQSAVALRCRVM 197
           DG+LV+PLSP+LSG +Q+VEL +D F  D+ ++EK  +  + K +NYVSQSAVALRCRVM
Sbjct: 132 DGILVNPLSPTLSGSQQIVELASDNFDADEGNLEKAIIPGVQKTKNYVSQSAVALRCRVM 191

Query: 198 PPPCMKNPYLNDASDIGVDPFGNQRTKCADFFPALFSGDGLSRYHTDFHEIKQIGTGNFS 257
           PPPC+KNPYL DAS++  DPFGNQR+KCA FFPA+  GDGLSRYHTDFHEI+QIGTG+FS
Sbjct: 192 PPPCIKNPYLKDASEVDTDPFGNQRSKCAGFFPAIIGGDGLSRYHTDFHEIEQIGTGHFS 251

Query: 258 RVFKVLKRIDGCLYAVKQSTRSLHQDIERRRALMEVQALAALGSHENIVGYYSSWFENEQ 317
           RVFKVLKRIDGCLYAVK STR LHQ+ ERR+ALMEVQALAALGSHEN VGYYSSWFENEQ
Sbjct: 252 RVFKVLKRIDGCLYAVKHSTRPLHQETERRKALMEVQALAALGSHENTVGYYSSWFENEQ 311

Query: 318 LYIQMELCDCSLSTGRYSHPFSEADALKALHQIAKALLFMHEKGVAHLDVKPDNIYVKDG 377
           LYIQMELCD SLS    +  F+E + L+A+HQ+AKAL F+HEKG+ HLDVKPDN+YVK+G
Sbjct: 312 LYIQMELCDYSLSRKSSTQLFTEGEFLRAMHQMAKALQFIHEKGIVHLDVKPDNVYVKNG 371

Query: 378 VYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEVVRGSTF 437
           VYKLGDFGCATLL+K+LP+EEGDARYMPQEILNE YD+LDKVDIFSLG  IYE++RGS  
Sbjct: 372 VYKLGDFGCATLLNKNLPVEEGDARYMPQEILNENYDHLDKVDIFSLGVTIYELIRGSPL 431

Query: 438 PETRFMNLKEGKLPLFPCHSLQFQNLIKAMVDPDPARRPSAREILQNPIFDKVRNHPKS 490
           PE+    L+EGKLPL   +SLQFQNL+K MVD DP RRPSA+E+++NPIFDK   + K+
Sbjct: 432 PESGPQFLREGKLPLLAGYSLQFQNLLKVMVDHDPVRRPSAKELVENPIFDKASKNTKA 476

[Close]

BLAST of CmaCh08G004070.1 vs. NCBI nr
Match: gi|1000941810|ref|XP_015582515.1| (PREDICTED: wee1-like protein kinase [Ricinus communis])

HSP 1 Score: 654.4 bits (1687), Expect = 1.5e-184
Identity = 331/495 (66.87%), Postives = 394/495 (79.60%), Query Frame = 1


Query: 4   KSKTLRTTMTATRK--KMKGTLTRHLQVQLGQVSLAPLHHQ---LSSSTAACFQDLPESE 63
           K KTL+ +    ++  KMKGTL RHLQVQLGQVS+     Q   L S  +   Q+L +SE
Sbjct: 2   KRKTLKRSNRKRKESDKMKGTLARHLQVQLGQVSVHQYQQQPSSLDSKPSTVLQNLLDSE 61

Query: 64  AM-ATVNDSGALPSSST---NFEDRDFILSQDFFCTPDYITPDNQNLLNGVDCYKESTPC 123
           A    +  S   PS      N +D+DFILSQDFFCTPDYITP +QNLLN  DC KE+  C
Sbjct: 62  ATDPQLQPSFGNPSIGDFDINVDDKDFILSQDFFCTPDYITPVDQNLLNSFDCNKENISC 121

Query: 124 PKSPEKINTVKTKRKIRDGVLVHPLSPSLSGREQVVELGNDTFGTDDEDMEKETM-AMPK 183
           P+SPEK+NT+KTK+  RDG+  +PLSP+LS ++  +E+  D  G D+  +EK T+    +
Sbjct: 122 PESPEKLNTIKTKKLRRDGISSNPLSPTLSNQQHNLEIVKDFVGMDEVIIEKTTVPGTQR 181

Query: 184 KQNYVSQSAVALRCRVMPPPCMKNPYLNDASDIGVDPFGNQRTKCADFFPALFSGDGLSR 243
            QNYVSQSAVALRCRVMPPPC+KNPYL +AS++ VDPFGNQ   C  FF A+  GDGLSR
Sbjct: 182 SQNYVSQSAVALRCRVMPPPCIKNPYLVEASEVEVDPFGNQXXXCTGFFSAIVGGDGLSR 241

Query: 244 YHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRSLHQDIERRRALMEVQALAALG 303
           YHTDFHEI+QIGTGNFSRVFKVLKRIDGCLYAVK STR LHQD ERR+ALMEVQALAALG
Sbjct: 242 YHTDFHEIQQIGTGNFSRVFKVLKRIDGCLYAVKLSTRKLHQDAERRKALMEVQALAALG 301

Query: 304 SHENIVGYYSSWFENEQLYIQMELCDCSLSTGRYSHPFSEADALKALHQIAKALLFMHEK 363
            H+NIVGYYSSWFENEQLYIQMELCDCSLS  ++S PF+EA  L+ LHQIAKAL F+HE+
Sbjct: 302 CHKNIVGYYSSWFENEQLYIQMELCDCSLSINKFSKPFTEAKVLEVLHQIAKALQFIHEQ 361

Query: 364 GVAHLDVKPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVD 423
           G+AHLDVKP+NIYVK+GVYKLGDFGCATLL++SLPIEEGDARYMPQEILNE Y +LDKVD
Sbjct: 362 GIAHLDVKPENIYVKNGVYKLGDFGCATLLNQSLPIEEGDARYMPQEILNENYSHLDKVD 421

Query: 424 IFSLGAAIYEVVRGSTFPE--TRFMNLKEGKLPLFPCHSLQFQNLIKAMVDPDPARRPSA 483
           IFSLGAAIYE+++GS  P+  ++F NL+EGKL L P HSLQFQNL+K MVDPDP  RPSA
Sbjct: 422 IFSLGAAIYELIKGSPLPQSGSQFFNLREGKLLLLPGHSLQFQNLLKIMVDPDPLNRPSA 481

Query: 484 REILQNPIFDKVRNH 487
           +E+++NPIF+KV+ +
Sbjct: 482 KEVVENPIFEKVQRN 496

[Close]

BLAST of CmaCh08G004070.1 vs. NCBI nr
Match: gi|590718548|ref|XP_007050841.1| (Kinase WEE1 isoform 1 [Theobroma cacao])

HSP 1 Score: 651.4 bits (1679), Expect = 1.3e-183
Identity = 329/492 (66.87%), Postives = 386/492 (78.46%), Query Frame = 1


Query: 1   MRRKSKTLRTTMTATRKKMKGTLTRHLQVQLGQVSLAPLHHQLSSSTAACF--QDLPESE 60
           M+RK     T       KMKGTL R LQVQL        HHQ SS+ ++    ++L +SE
Sbjct: 1   MKRKVVKRGTNRRRESGKMKGTLGRLLQVQLH-------HHQSSSANSSSLFARNLLDSE 60

Query: 61  AMATVNDSGALPSSSTNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDCYKESTPCPKSP 120
                  + AL   +   +D+DFILSQDFFCTPDYITPDNQNL+NG DC K++ PCPKSP
Sbjct: 61  PDDPSTSASALDLDAA--DDKDFILSQDFFCTPDYITPDNQNLMNGFDCNKDNIPCPKSP 120

Query: 121 EKINTVKTKRKIRDGVLVHPLSPSLSGREQVVELGNDTFGTDDEDMEKETM-AMPKKQNY 180
           EK++T+K+KR  +DG+ V+PLSP LSG +Q+VEL  D F  D  ++EK  +  + K +NY
Sbjct: 121 EKLHTLKSKRPRQDGIQVNPLSPILSGSQQIVELAKDNFDVDVVNLEKTVIPGVQKTKNY 180

Query: 181 VSQSAVALRCRVMPPPCMKNPYLNDASDIGVDPFGNQRTKCADFFPALFSGDGLSRYHTD 240
           VSQSAVALRCRVMPPPC+KNPYL DAS++  DP GNQR+KCA FFPA+  GDGLSRYHTD
Sbjct: 181 VSQSAVALRCRVMPPPCIKNPYLKDASEMDTDPLGNQRSKCAGFFPAIIGGDGLSRYHTD 240

Query: 241 FHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRSLHQDIERRRALMEVQALAALGSHEN 300
           FHEI+QIGTGNFSRVFKVLKRIDGCLYAVK +TR LHQD ERR+ALMEVQALAALGSHEN
Sbjct: 241 FHEIEQIGTGNFSRVFKVLKRIDGCLYAVKHTTRQLHQDTERRKALMEVQALAALGSHEN 300

Query: 301 IVGYYSSWFENEQLYIQMELCDCSLSTGRYSHPFSEADALKALHQIAKALLFMHEKGVAH 360
           IVGYYSSWFENEQLYIQMELCD SLS       F+E + L+A  QIAKAL  +HE+G+AH
Sbjct: 301 IVGYYSSWFENEQLYIQMELCDYSLSRNTSLQLFTEGEVLRAWLQIAKALQCIHERGIAH 360

Query: 361 LDVKPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSL 420
           LDVKPDNIYVK+G YKLGDFGCATLL+KSLPIEEGDARYMPQEILNE YD+LDKVDIFSL
Sbjct: 361 LDVKPDNIYVKNGAYKLGDFGCATLLNKSLPIEEGDARYMPQEILNENYDHLDKVDIFSL 420

Query: 421 GAAIYEVVRGSTFPETRFMNLKEGKLPLFPCHSLQFQNLIKAMVDPDPARRPSAREILQN 480
           G  IYE++RGS   E+    L+EGKLPL P HS+QFQNL+K MVD DP RRPSA+E+++N
Sbjct: 421 GVTIYELIRGSPLQESGRQFLREGKLPLLPGHSIQFQNLLKVMVDQDPVRRPSAKELIEN 480

Query: 481 PIFDKVRNHPKS 490
           PIFDKV N+ K+
Sbjct: 481 PIFDKVSNNAKA 483

The following BLAST results are available for this feature:

BLAST of CmaCh08G004070.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
WEE1_ARATH	3.9e-164	60.04	Wee1-like protein kinase OS=Arabidopsis thaliana GN=WEE1 PE=1 SV=1	[more]
WEE1_ORYSJ	2.4e-137	57.67	Wee1-like protein kinase OS=Oryza sativa subsp. japonica GN=WEE1 PE=3 SV=1	[more]
WEE1B_XENLA	2.7e-48	35.74	Wee1-like protein kinase 1-B OS=Xenopus laevis GN=wee1-b PE=1 SV=1	[more]
WEE1_DROME	2.3e-47	35.59	Wee1-like protein kinase OS=Drosophila melanogaster GN=Wee1 PE=1 SV=2	[more]
WEE1A_XENLA	3.0e-47	36.10	Wee1-like protein kinase 1-A OS=Xenopus laevis GN=wee1-a PE=2 SV=1	[more]

BLAST of CmaCh08G004070.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K5B3_CUCSA	8.0e-249	87.12	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G395770 PE=3 SV=1	[more]
A0A0D2SJ55_GOSRA	5.6e-186	67.43	Uncharacterized protein OS=Gossypium raimondii GN=B456_007G129700 PE=3 SV=1	[more]
A0A061DY89_THECC	9.0e-184	66.87	Kinase WEE1 isoform 1 OS=Theobroma cacao GN=TCM_004583 PE=3 SV=1	[more]
M5VNA9_PRUPE	4.9e-182	68.00	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa015605mg PE=3 SV=1	[more]
D7TP05_VITVI	2.4e-181	67.51	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_07s0104g01740 PE=3 SV=...	[more]

BLAST of CmaCh08G004070.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G02970.1	2.2e-165	60.04	WEE1 kinase homolog	[more]
AT4G19110.2	4.3e-20	31.22	Protein kinase superfamily protein	[more]
AT3G20860.1	5.6e-20	29.73	NIMA-related kinase 5	[more]
AT5G45430.1	5.6e-20	30.69	Protein kinase superfamily protein	[more]
AT1G18890.1	1.6e-19	27.24	calcium-dependent protein kinase 1	[more]

BLAST of CmaCh08G004070.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659101452\|ref\|XP_008451611.1\|	2.7e-250	87.93	PREDICTED: wee1-like protein kinase [Cucumis melo]	[more]
gi\|449436531\|ref\|XP_004136046.1\|	1.1e-248	87.12	PREDICTED: wee1-like protein kinase [Cucumis sativus]	[more]
gi\|823188157\|ref\|XP_012490421.1\|	8.1e-186	67.43	PREDICTED: wee1-like protein kinase [Gossypium raimondii]	[more]
gi\|1000941810\|ref\|XP_015582515.1\|	1.5e-184	66.87	PREDICTED: wee1-like protein kinase [Ricinus communis]	[more]
gi\|590718548\|ref\|XP_007050841.1\|	1.3e-183	66.87	Kinase WEE1 isoform 1 [Theobroma cacao]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR000719	Prot_kinase_dom
IPR008271	Ser/Thr_kinase_AS
IPR011009	Kinase-like_dom_sf
IPR017441	Protein_kinase_ATP_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0004672	protein kinase activity
GO:0005524	ATP binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006468	protein phosphorylation

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
biological_process	GO:0009069	serine family amino acid metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity
molecular_function	GO:0004674	protein serine/threonine kinase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh08G004070	CmaCh08G004070	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh08G004070.1	CmaCh08G004070.1-protein	polypeptide

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh08G004070.1.three_prime_UTR.1	CmaCh08G004070.1.three_prime_UTR.1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh08G004070.1.CDS.10	CmaCh08G004070.1.CDS.10	CDS
CmaCh08G004070.1.CDS.9	CmaCh08G004070.1.CDS.9	CDS
CmaCh08G004070.1.CDS.8	CmaCh08G004070.1.CDS.8	CDS
CmaCh08G004070.1.CDS.7	CmaCh08G004070.1.CDS.7	CDS
CmaCh08G004070.1.CDS.6	CmaCh08G004070.1.CDS.6	CDS
CmaCh08G004070.1.CDS.5	CmaCh08G004070.1.CDS.5	CDS
CmaCh08G004070.1.CDS.4	CmaCh08G004070.1.CDS.4	CDS
CmaCh08G004070.1.CDS.3	CmaCh08G004070.1.CDS.3	CDS
CmaCh08G004070.1.CDS.2	CmaCh08G004070.1.CDS.2	CDS
CmaCh08G004070.1.CDS.1	CmaCh08G004070.1.CDS.1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh08G004070.1.five_prime_UTR.1	CmaCh08G004070.1.five_prime_UTR.1	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh08G004070.1.exon.10	CmaCh08G004070.1.exon.10	exon
CmaCh08G004070.1.exon.9	CmaCh08G004070.1.exon.9	exon
CmaCh08G004070.1.exon.8	CmaCh08G004070.1.exon.8	exon
CmaCh08G004070.1.exon.7	CmaCh08G004070.1.exon.7	exon
CmaCh08G004070.1.exon.6	CmaCh08G004070.1.exon.6	exon
CmaCh08G004070.1.exon.5	CmaCh08G004070.1.exon.5	exon
CmaCh08G004070.1.exon.4	CmaCh08G004070.1.exon.4	exon
CmaCh08G004070.1.exon.3	CmaCh08G004070.1.exon.3	exon
CmaCh08G004070.1.exon.2	CmaCh08G004070.1.exon.2	exon
CmaCh08G004070.1.exon.1	CmaCh08G004070.1.exon.1	exon

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 238..480 score: 2.9
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 238..480 score: 2.6
IPR000719	Protein kinase domain	PROFILE	PS50011	PROTEIN_KINASE_DOM	coord: 238..480 score: 36
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 355..367 scor
IPR011009	Protein kinase-like domain	unknown	SSF56112	Protein kinase-like (PK-like)	coord: 231..484 score: 1.89
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 244..267 scor
None	No IPR available	GENE3D	G3DSA:1.10.510.10		coord: 307..485 score: 4.0
None	No IPR available	GENE3D	G3DSA:3.30.200.20		coord: 232..306 score: 2.7
None	No IPR available	PANTHER	PTHR11042	EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED	coord: 51..485 score: 8.3E
None	No IPR available	PANTHER	PTHR11042:SF69	WEE1-LIKE PROTEIN KINASE	coord: 51..485 score: 8.3E