CmaCh08G000030.1 (mRNA) Cucurbita maxima (Rimu)

NameCmaCh08G000030.1
TypemRNA
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionProton pump interactor, putative
LocationCma_Chr08 : 22743 .. 25388 (+)
Sequence length1866
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTAATCCGACTAAAGCTACCATTTTCTGCCATTAACTGGGCCCATTTGTAGCACCCATTGTTTTGATTTTGAGGTATCACACGTCTTCTCTCTTTTTCTTGCTCTGTTTTTCCCCTGTAGTTTCAGTAGTTAGGAAAATTTTCGATTGCAACAATAAATTTAATGTGTAAGTTTAGAATTGGATGCATTTTCATTCCGCGTTTTTCAATGGCTTTTTCCTAGCTCGAGGTTGTTTTTGAATTAGGCAAAGAAAAGCGGCATGGAAGAAGGTGAATTATATTGTAAAGAGAAGAAGGTGAGGGAGGAGGGATCAGAAGTTGCTGGTGAAGTGGCGATATTAGATTATTCTGATGATGAACGCCAATTGTTCGATGAAATGCGTGTTCGAGGTCGGCATTTTTATTTTGCCAAGGTTTTGCCAATTGAGAACCCAAAGATGGATGCCATGATTAAGAAGGCTGAAGAGACGATTGAGAAGATCAATCGAGATCAAGTTGTGATGGCCCAAAAGATAAGGGAAAGGATGGTATGTGAAAAGAACCCTAGTTTTTTTCATTTTAGAACATTGTAGTTAAGTCTCCTGGTTTGGCACTTGCTTATGTGAGCTCTGAATAATGGAGGATTGGAATGAATTTTGTTCATAGATGGATCGTGACGCACTGCGCTCGAAATTGAACCGGATGAATTTTGGTAATTATGAACTCGCCCTAAAATGGAAGAGAAATAGTTTGGACTTCCTGTATCCCTCTCTAGACAAACTGACTTTTGCAAACAATGCATATAAAGGGAAATCGGTTAACTCGTGCTTGTCGGTAGGGGAAATTGATAAGCAAGTATGGTCAATTGAACTCTTTCTTGCGTATTTTTGCAACACTTCCGATCTTCTTCATCTTATGCAGTTTATTTATCGTTGTGTGACCAGAAACTGTGTTTCTTGATGGTACATGGCTGTAAAAACATGGGTGACGAGAGAAAGCTATTAAGGGAAGTCAATGCAATGCAAGGGGAGGATGGTGGCATGACATTAGATGAACTCCGTGCTCCTGTAATATTCAAACTTGAGCGTCAATACAATATTCAATTTTACTTGTCGTTCTTAATGTCATGACCATTACTACTTGATGAATTATCATCTCTCTAAAGCTTCTGCGTTCTCATGAATAGATTCAACACTTGCAACAACGATTGTCCATCAACTATGTGGCGCATACAGAAATCGATGAAAATGCTCGTAGTGAAGTCATTCTCAATGCCGAAAAGCAACATGAAATTATTAGGGAGACAGCATTGGCTAATGCTGTAGTGAATGGAAAATTGTGGAACTCCCTTGGCTCGAAAAAGTCCATTCAACAGCAAGTAAGAGCCAAAACTAAAATTTAGGAGGGTTCTTTGACTGTATAGCTTACAACTAAAAAAATCTCGATGTTTTTGTACGTTGAAAGGTTCAGGAACTCAACAATAGATCATGTGAACTAAGGGAAAGACAGAGGGAAGTAAATGTCAAAGTTAGGAAAGTAAATAAAGAAGTTAAGATGATAGAAAAAGATATACGTTCTCTACAAAAGCTCTTCACGTATGCAAATCGCAAAAAGGACGAGGCATACAACACCATTCTTAAACTGAAGAGGCAGTACGGGGAGGAGGTACCTATGATTACAAGCTCTAAATACTTTTATTGCATACTTATTTATTTCGTTTGATGTGAGTTCAGTTGTACTGATATATATTGTGGCATTTCGTGTTAGAATGCTTCCTACTACCAATATCGTTCCCTCATGCAGAAGGTCCAAGCACTTGTTAAGAAAAAAGATACCGCAGCCATTGAAGAATTATCTCAAACACAGGTGTTTCCTATATCCCTTGTCTGTTTATGAAGTTGTAGGGGGTTATTGTGTTTTTGTCAACAACTTTTGAAAGCTGATTTTTCCATTACAACCAGGTTGAAAAGTTCATGCAACAGTGGAACAATAACCTAGATTTCAGAAACGATTACAAAAAGAGAGCTATCCCGCTTCTAAACAACCGTCATCTTGGCGTGGATGGGAGTATGATCACCGACCAGAAGGCAGAGGTTAAGGACTCTAAAAAGGCTCCTAAACCAGAGGCGCTTTCTAAAGCTAGATTGAAATGGTTGATGAAAGATCCTGAAGATCCCTCCGAACTTCTTTTTCGGTGAAACTAAAGCGATGAACCCCTTTGCACATATGCTTCAAGTTTCAACTTTGATCCATTTATTCCTTAAAATTCTCCTTTTATTGAACAACATTTCTCCCGCATCATGTTCAGAAGTGTTTTACAAAGCTAGTTTTTCTGTGAAGTCATTTCCCCACTTGGAACTTTTGTTTGGAAGCCTATGAATTCAAAAAAAAACCAAACCAAACCTTGCTATGTTGATGTTCCTTTGTGACATGAATGTGCCACCTACTGTAGCAGAATGAAGATTGTCCCTCCATATTAACTTGCTAGGGAAATCATTCATATGTTTTCTTATATAAATCTCATAGAAAATCACAAGCACAGCTCTAAAAGGAAAGATACGACAGATAGGATTCACATTGAATTTAGAATTAAGTTAGTTAATTATGTAATTTTATTTTGAAAAAATAAAAATAAATAAATGAGGCATCATTGGAAGAGAGAGAGAGA

mRNA sequence

TTAATCCGACTAAAGCTACCATTTTCTGCCATTAACTGGGCCCATTTGTAGCACCCATTGTTTTGATTTTGAGGCAAAGAAAAGCGGCATGGAAGAAGGTGAATTATATTGTAAAGAGAAGAAGGTGAGGGAGGAGGGATCAGAAGTTGCTGGTGAAGTGGCGATATTAGATTATTCTGATGATGAACGCCAATTGTTCGATGAAATGCGTGTTCGAGGTCGGCATTTTTATTTTGCCAAGGTTTTGCCAATTGAGAACCCAAAGATGGATGCCATGATTAAGAAGGCTGAAGAGACGATTGAGAAGATCAATCGAGATCAAGTTGTGATGGCCCAAAAGATAAGGGAAAGGATGATGGATCGTGACGCACTGCGCTCGAAATTGAACCGGATGAATTTTGGTAATTATGAACTCGCCCTAAAATGGAAGAGAAATAGTTTGGACTTCCTGTATCCCTCTCTAGACAAACTGACTTTTGCAAACAATGCATATAAAGGGAAATCGGTTAACTCGTGCTTGTCGGTAGGGGAAATTGATAAGCAAAAACTGTGTTTCTTGATGGTACATGGCTGTAAAAACATGGGTGACGAGAGAAAGCTATTAAGGGAAGTCAATGCAATGCAAGGGGAGGATGGTGGCATGACATTAGATGAACTCCGTGCTCCTATTCAACACTTGCAACAACGATTGTCCATCAACTATGTGGCGCATACAGAAATCGATGAAAATGCTCGTAGTGAAGTCATTCTCAATGCCGAAAAGCAACATGAAATTATTAGGGAGACAGCATTGGCTAATGCTGTAGTGAATGGAAAATTGTGGAACTCCCTTGGCTCGAAAAAGTCCATTCAACAGCAAGTTCAGGAACTCAACAATAGATCATGTGAACTAAGGGAAAGACAGAGGGAAGTAAATGTCAAAGTTAGGAAAGTAAATAAAGAAGTTAAGATGATAGAAAAAGATATACGTTCTCTACAAAAGCTCTTCACGTATGCAAATCGCAAAAAGGACGAGGCATACAACACCATTCTTAAACTGAAGAGGCAGTACGGGGAGGAGAATGCTTCCTACTACCAATATCGTTCCCTCATGCAGAAGGTCCAAGCACTTGTTAAGAAAAAAGATACCGCAGCCATTGAAGAATTATCTCAAACACAGGTTGAAAAGTTCATGCAACAGTGGAACAATAACCTAGATTTCAGAAACGATTACAAAAAGAGAGCTATCCCGCTTCTAAACAACCGTCATCTTGGCGTGGATGGGAGTATGATCACCGACCAGAAGGCAGAGGTTAAGGACTCTAAAAAGGCTCCTAAACCAGAGGCGCTTTCTAAAGCTAGATTGAAATGGTTGATGAAAGATCCTGAAGATCCCTCCGAACTTCTTTTTCGGTGAAACTAAAGCGATGAACCCCTTTGCACATATGCTTCAAGTTTCAACTTTGATCCATTTATTCCTTAAAATTCTCCTTTTATTGAACAACATTTCTCCCGCATCATGTTCAGAAGTGTTTTACAAAGCTAGTTTTTCTGTGAAGTCATTTCCCCACTTGGAACTTTTGTTTGGAAGCCTATGAATTCAAAAAAAAACCAAACCAAACCTTGCTATGTTGATGTTCCTTTGTGACATGAATGTGCCACCTACTGTAGCAGAATGAAGATTGTCCCTCCATATTAACTTGCTAGGGAAATCATTCATATGTTTTCTTATATAAATCTCATAGAAAATCACAAGCACAGCTCTAAAAGGAAAGATACGACAGATAGGATTCACATTGAATTTAGAATTAAGTTAGTTAATTATGTAATTTTATTTTGAAAAAATAAAAATAAATAAATGAGGCATCATTGGAAGAGAGAGAGAGA

Coding sequence (CDS)

ATGGAAGAAGGTGAATTATATTGTAAAGAGAAGAAGGTGAGGGAGGAGGGATCAGAAGTTGCTGGTGAAGTGGCGATATTAGATTATTCTGATGATGAACGCCAATTGTTCGATGAAATGCGTGTTCGAGGTCGGCATTTTTATTTTGCCAAGGTTTTGCCAATTGAGAACCCAAAGATGGATGCCATGATTAAGAAGGCTGAAGAGACGATTGAGAAGATCAATCGAGATCAAGTTGTGATGGCCCAAAAGATAAGGGAAAGGATGATGGATCGTGACGCACTGCGCTCGAAATTGAACCGGATGAATTTTGGTAATTATGAACTCGCCCTAAAATGGAAGAGAAATAGTTTGGACTTCCTGTATCCCTCTCTAGACAAACTGACTTTTGCAAACAATGCATATAAAGGGAAATCGGTTAACTCGTGCTTGTCGGTAGGGGAAATTGATAAGCAAAAACTGTGTTTCTTGATGGTACATGGCTGTAAAAACATGGGTGACGAGAGAAAGCTATTAAGGGAAGTCAATGCAATGCAAGGGGAGGATGGTGGCATGACATTAGATGAACTCCGTGCTCCTATTCAACACTTGCAACAACGATTGTCCATCAACTATGTGGCGCATACAGAAATCGATGAAAATGCTCGTAGTGAAGTCATTCTCAATGCCGAAAAGCAACATGAAATTATTAGGGAGACAGCATTGGCTAATGCTGTAGTGAATGGAAAATTGTGGAACTCCCTTGGCTCGAAAAAGTCCATTCAACAGCAAGTTCAGGAACTCAACAATAGATCATGTGAACTAAGGGAAAGACAGAGGGAAGTAAATGTCAAAGTTAGGAAAGTAAATAAAGAAGTTAAGATGATAGAAAAAGATATACGTTCTCTACAAAAGCTCTTCACGTATGCAAATCGCAAAAAGGACGAGGCATACAACACCATTCTTAAACTGAAGAGGCAGTACGGGGAGGAGAATGCTTCCTACTACCAATATCGTTCCCTCATGCAGAAGGTCCAAGCACTTGTTAAGAAAAAAGATACCGCAGCCATTGAAGAATTATCTCAAACACAGGTTGAAAAGTTCATGCAACAGTGGAACAATAACCTAGATTTCAGAAACGATTACAAAAAGAGAGCTATCCCGCTTCTAAACAACCGTCATCTTGGCGTGGATGGGAGTATGATCACCGACCAGAAGGCAGAGGTTAAGGACTCTAAAAAGGCTCCTAAACCAGAGGCGCTTTCTAAAGCTAGATTGAAATGGTTGATGAAAGATCCTGAAGATCCCTCCGAACTTCTTTTTCGGTGA

Protein sequence

MEEGELYCKEKKVREEGSEVAGEVAILDYSDDERQLFDEMRVRGRHFYFAKVLPIENPKMDAMIKKAEETIEKINRDQVVMAQKIRERMMDRDALRSKLNRMNFGNYELALKWKRNSLDFLYPSLDKLTFANNAYKGKSVNSCLSVGEIDKQKLCFLMVHGCKNMGDERKLLREVNAMQGEDGGMTLDELRAPIQHLQQRLSINYVAHTEIDENARSEVILNAEKQHEIIRETALANAVVNGKLWNSLGSKKSIQQQVQELNNRSCELRERQREVNVKVRKVNKEVKMIEKDIRSLQKLFTYANRKKDEAYNTILKLKRQYGEENASYYQYRSLMQKVQALVKKKDTAAIEELSQTQVEKFMQQWNNNLDFRNDYKKRAIPLLNNRHLGVDGSMITDQKAEVKDSKKAPKPEALSKARLKWLMKDPEDPSELLFR
BLAST of CmaCh08G000030.1 vs. Swiss-Prot
Match: PPI1_ARATH (Proton pump-interactor 1 OS=Arabidopsis thaliana GN=PPI1 PE=1 SV=2)

HSP 1 Score: 98.6 bits (244), Expect = 1.8e-19
Identity = 79/279 (28.32%), Postives = 143/279 (51.25%), Query Frame = 1

Query: 161 GCKNMGDERK-----LLREVNAMQGEDGGMTL--------DELRAPIQHLQQRLSINYVA 220
           G   M DE++     L + +  ++  DGG           +EL + I   Q R+    + 
Sbjct: 140 GFNTMFDEKRKEMEPLQQALGKLRSNDGGSARGPAICSSEEELNSMIYSYQYRIQHESIP 199

Query: 221 HTEIDENARSEVILNAEKQHEIIRETALANAVVNGKLWNSLGSKKSIQQQVQELNNRSCE 280
            TE       + IL   +  E  R+  +ANA +  K+  S+G K  IQ QV+ +      
Sbjct: 200 LTE------EKQILKEIRLLEGTRDKVIANAAMRAKIKESMGQKDDIQGQVKLMGAGLDG 259

Query: 281 LRERQREVNVKVRKVNKEVKMIEKDIRSLQKLFTYANRKKDEAYNTILKLKRQYGEENAS 340
           +++ ++ ++ ++ ++++++K  + +I  L+      + K+D+AY+ I  L+RQ  E N+ 
Sbjct: 260 VKKERQAISARINELSEKLKATKDEITVLENELKTVSEKRDKAYSNIHDLRRQRDETNSE 319

Query: 341 YYQYRSLMQKVQALVKKKDTAAIEELSQTQVEKFMQQWNNNLDFRNDYKKRAIPLLNNRH 400
           YYQ R+++ K + L  +K+ + +E L+  +VEKF+  W +  +FR DY+KR +  L++R 
Sbjct: 320 YYQNRTVLNKARDLAAQKNISELEALANAEVEKFISLWCSKKNFREDYEKRILQSLDSRQ 379

Query: 401 LGVDGSMIT-DQK----AEVKDSKKAP-KPEALSKARLK 421
           L  DG M   D+K     E   SK  P + E + KA+ K
Sbjct: 380 LSRDGRMRNPDEKPLIAPEAAPSKATPSETEVVPKAKAK 412

BLAST of CmaCh08G000030.1 vs. Swiss-Prot
Match: PPI2_ARATH (Proton pump-interactor 2 OS=Arabidopsis thaliana GN=PPI2 PE=2 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 6.8e-11
Identity = 53/205 (25.85%), Postives = 106/205 (51.71%), Query Frame = 1

Query: 205 YVAHTEIDENARSEVILNAEKQHEIIRETALANAVVNGKLWNSLGSKKSIQQQVQELNNR 264
           Y+AH +I+  +     +  E++  +++ET  A+ +V  +  +SL  K++   +V+ +   
Sbjct: 172 YIAHYQIEHGS-----IGFEEEDWVLKETEKADGIVLSE--DSLAEKEASINRVKSMAVE 231

Query: 265 SCELRERQREVNVKVRKVNKEVKMIEKDIRSLQKLFTYANRKKDEAYNTILKLKRQYGEE 324
             E+++    +  K+  ++ +V   + ++R L     +   ++D +Y  I  L+ Q  + 
Sbjct: 232 LNEVKKELDAITWKINHLSDKVGKSQNNLRVLDVKKAHILEERDRSYERIKMLRIQRDKG 291

Query: 325 NASYYQYRSLMQKVQALVKKKDTAAIEELSQTQVEKFMQQWNNNLDFRNDYKKRAIPLLN 384
            A++YQ   +M+K + L    +   +E  + ++ ++FM QWN++  FR+DY KR  P L 
Sbjct: 292 KAAFYQSLPVMRKARELAASGNVRDLEVFASSEADRFMTQWNDDKAFRDDYVKRISPSLC 351

Query: 385 NRHLGVDGSMITDQKAEVKDSKKAP 410
            R L  DG  I D + +V   KK P
Sbjct: 352 ERQLNQDG-RIKDPEVQVVWEKKVP 368

BLAST of CmaCh08G000030.1 vs. Swiss-Prot
Match: PPI4_ARATH (Proton pump-interactor 4 OS=Arabidopsis thaliana GN=PPI4 PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.5e-10
Identity = 41/125 (32.80%), Postives = 71/125 (56.80%), Query Frame = 1

Query: 267 ELRERQREVNVKVRKVNKEVKMIEKDIRSLQKLFTYANRKKDEAYNTILKLKRQYGEENA 326
           E+ + Q  V  ++ ++ + +  I+ +I  L         ++D+A   I  L+ Q  + NA
Sbjct: 114 EVEKEQEVVRGRIYRLGERLSEIKMEIELLDVQMACVLNQRDKAVERIKFLRMQRDKGNA 173

Query: 327 SYYQYRSLMQKVQALVKKKDTAAIEELSQTQVEKFMQQWNNNLDFRNDYKKRAIPLLNNR 386
           ++YQ R +M+K   L    +   +EEL+ ++VEKFM +WNN+  FR++YKKR +P +N R
Sbjct: 174 AFYQSRVVMKKAIELAASGNVRDLEELADSEVEKFMSRWNNDKAFRDNYKKRILPSVNER 233

Query: 387 HLGVD 392
            L  D
Sbjct: 234 KLRCD 238

BLAST of CmaCh08G000030.1 vs. Swiss-Prot
Match: PPI3A_ARATH (Proton pump-interactor 3A OS=Arabidopsis thaliana GN=PPI3A PE=3 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 7.8e-07
Identity = 49/207 (23.67%), Postives = 103/207 (49.76%), Query Frame = 1

Query: 203 INYVAHTEIDENARSEVILNAEKQHEIIRETALANAVVNGKLWNSLGSKKSIQQQVQELN 262
           ++Y+A  +I+  +     +  E++  +++ET   + ++  +   S+   KS+  ++ E+ 
Sbjct: 170 LSYIAQYQIEYGS-----IGLEEEDWMLKETEKPDGIILSEKEASINRVKSMALELNEVK 229

Query: 263 NRSCELRERQREVNVKVRKVNKEVKMIEKDIRSLQKLFTYANRKKDEAYNTILKLKRQYG 322
           N    +  +  +++ K+ K    +++++     L+K   +   K+D  Y  I  L+ Q  
Sbjct: 230 NELDAITWKINDLSDKLWKSQNNIRVLD-----LEK--AHILEKRDRFYARIKMLRIQRD 289

Query: 323 EENASYYQYRSLMQKVQALVKKKDTAAIEELSQTQVEKFMQQWNNNLDFRNDYKKRAIPL 382
           + NA+++Q  S+M K + L    +   +E  + ++V++FM  WN++  FR DY +R    
Sbjct: 290 KGNAAFFQSLSVMCKAKELAASGNVRELEVFASSEVDRFMTLWNDDKAFREDYVRRISHS 349

Query: 383 LNNRHLGVDGSMITDQKAEVKDSKKAP 410
           L  R L  DG  I D   ++   KK P
Sbjct: 350 LCERELNEDG-RIKDADLQIFWEKKVP 363

BLAST of CmaCh08G000030.1 vs. Swiss-Prot
Match: PPI3B_ARATH (Proton pump-interactor 3B OS=Arabidopsis thaliana GN=PPI3B PE=3 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 7.8e-07
Identity = 49/207 (23.67%), Postives = 103/207 (49.76%), Query Frame = 1

Query: 203 INYVAHTEIDENARSEVILNAEKQHEIIRETALANAVVNGKLWNSLGSKKSIQQQVQELN 262
           ++Y+A  +I+  +     +  E++  +++ET   + ++  +   S+   KS+  ++ E+ 
Sbjct: 170 LSYIAQYQIEYGS-----IGLEEEDWMLKETEKPDGIILSEKEASINRVKSMALELNEVK 229

Query: 263 NRSCELRERQREVNVKVRKVNKEVKMIEKDIRSLQKLFTYANRKKDEAYNTILKLKRQYG 322
           N    +  +  +++ K+ K    +++++     L+K   +   K+D  Y  I  L+ Q  
Sbjct: 230 NELDAITWKINDLSDKLWKSQNNIRVLD-----LEK--AHILEKRDRFYARIKMLRIQRD 289

Query: 323 EENASYYQYRSLMQKVQALVKKKDTAAIEELSQTQVEKFMQQWNNNLDFRNDYKKRAIPL 382
           + NA+++Q  S+M K + L    +   +E  + ++V++FM  WN++  FR DY +R    
Sbjct: 290 KGNAAFFQSLSVMCKAKELAASGNVRELEVFASSEVDRFMTLWNDDKAFREDYVRRISHS 349

Query: 383 LNNRHLGVDGSMITDQKAEVKDSKKAP 410
           L  R L  DG  I D   ++   KK P
Sbjct: 350 LCERELNEDG-RIKDADLQIFWEKKVP 363

BLAST of CmaCh08G000030.1 vs. TrEMBL
Match: A0A0A0K9L8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G094680 PE=4 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 2.3e-154
Identity = 296/438 (67.58%), Postives = 352/438 (80.37%), Query Frame = 1

Query: 1   MEEGELY-CKEKKVREE--GSEVAGEVAILDYSDDERQLFDEMRVRGRHFYFAKVLPIEN 60
           MEEG+ + C+EK + EE  GSEV GE  I+D SDDE  +        RHFYF KVLP+EN
Sbjct: 31  MEEGDQFNCREKNMVEEQEGSEVIGEEGIIDCSDDEMPVGVV-----RHFYFVKVLPLEN 90

Query: 61  PKMDAMIKKAEETIEKINRDQVVMAQKIRERMMDRDALRSKLNRMN-FGNYELALKWKRN 120
           P +DAMIKKAEE I+K NRDQV++A KIRE+MMDRDA+RSKL+ M  +  YEL +KW + 
Sbjct: 91  PNLDAMIKKAEEMIDKTNRDQVLLATKIREKMMDRDAVRSKLSWMKKYDYYELTIKWHKE 150

Query: 121 SLDFLYPSLDKLTFANNAYKGKSVNSCLSVGEIDKQKLCFLMVHGCKNMGDERKLLREVN 180
            LD L+ SLDKLTFANNAYKGK VNSCLS GE+DKQKL FLM+HGCKNM DERKLLREVN
Sbjct: 151 RLDILHLSLDKLTFANNAYKGKPVNSCLSSGEVDKQKLHFLMLHGCKNMADERKLLREVN 210

Query: 181 AMQGEDGGMTLDELRAPIQHLQQRLSINYVAHTEIDENARSEVILNAEKQHEIIRETALA 240
           A QG+DGG+TLDEL APIQ L+ +   NY+  TE DENAR + IL   K HE+I E A+A
Sbjct: 211 ASQGKDGGITLDELHAPIQRLRGQFPTNYMGRTESDENARKQAILKGIKHHEVIMEKAIA 270

Query: 241 NAVVNGKLWNSLGSKKSIQQQVQELNNRSCELRERQREVNVKVRKVNKEVKMIEKDIRSL 300
           NAVVNGKLWNSL SKKSIQ++++ LNN S + RERQR+   ++RKVN E++ +EKDIRSL
Sbjct: 271 NAVVNGKLWNSLSSKKSIQEEIEMLNNSSIQPRERQRKTITEIRKVNLELEKVEKDIRSL 330

Query: 301 QKLFTYANRKKDEAYNTILKLKRQYGEENASYYQYRSLMQKVQALVKKKDTAAIEELSQT 360
           QKLFT ANRKKD+AY TIL+LK+QYGEENASYYQYRSLM+KV+ALVKKKD AA++E+SQT
Sbjct: 331 QKLFTDANRKKDDAYTTILRLKKQYGEENASYYQYRSLMKKVEALVKKKDIAAVQEVSQT 390

Query: 361 QVEKFMQQWNNNLDFRNDYKKRAIPLLNNRHLGVDGSM-ITDQKAEVKDSKKAPKPEALS 420
           QVEKFM QWN+N++FRNDYKKR  P L NRHLGVDG M + +QK EV+D++K  KP+ LS
Sbjct: 391 QVEKFMHQWNDNMEFRNDYKKRVNPSLKNRHLGVDGRMTMNNQKPEVEDTRKIIKPDTLS 450

Query: 421 KARLKWLMKDPEDPSELL 434
           K RLKWLMKD EDP ELL
Sbjct: 451 KTRLKWLMKDSEDPFELL 463

BLAST of CmaCh08G000030.1 vs. TrEMBL
Match: A0A0A0KDK3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G094700 PE=4 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 1.8e-87
Identity = 197/404 (48.76%), Postives = 263/404 (65.10%), Query Frame = 1

Query: 1   MEEGELYCKEKKVREEGSEVAGEVAILDYSDDERQLFDEMRVRGRHFYFAKVLPIENPKM 60
           M EG+    E K+ EEGSEVAG+ A++D    E  +FD++ V  R FYF KV  +EN   
Sbjct: 75  MGEGDCDFGENKLVEEGSEVAGDEAVVD----EHLVFDDVPVGVRQFYFVKVKALENSNT 134

Query: 61  DAMIKKAEETIEKINRDQVVMAQKIRERMMDRDALRSKLNRMNFGNY--ELALKWKRNSL 120
           DAMIK AEETI K+N+DQ+++AQKIR+R MDRD + +KLN   +     +  L   RN +
Sbjct: 135 DAMIK-AEETISKMNQDQILIAQKIRDRRMDRDQVIAKLNDQQYYESWDDFILLCNRNKM 194

Query: 121 DFLYPSLDKLTFANNAYKGKSVNSCLSVGEIDKQKLCFLMVHGCKNMGDERKLLREVNAM 180
           + L+ SL+K+T+ N A+KGK   SCLS G++DKQ+L FLM HGCKNM DERKLL+EV A 
Sbjct: 195 NILHFSLEKMTYVNKAHKGKPNISCLSGGKVDKQELSFLMRHGCKNMADERKLLKEVKAR 254

Query: 181 QGEDGGMTLDELRAPIQHLQQRLSINYVAHTEIDENARSEVILNAEKQHEIIRETALANA 240
           Q  DGG T+DE+ + ++ L   L +                 L  +  H I +   + + 
Sbjct: 255 QENDGGSTVDEVDSSLESLFGNLWLKP---------------LGFQITHAIKKIEGIDDD 314

Query: 241 VVNGKLWNSLGSKKSIQQQVQELNNRSCELRERQREVNVKVRKVNKEVKMIEKDIRSLQK 300
            V G+LW+S          +   ++ S  +R RQ     K +K  KE++ +EKDI SLQK
Sbjct: 315 PVEGELWSS---------SLARTHDSSDYIRRRQARFRSKAKKTTKELEKVEKDISSLQK 374

Query: 301 LFTYANRKKDEAYNTILKLKRQYGEENASYYQYRSLMQKVQALVKKKDTAAIEELSQTQV 360
            F Y NR+K EAY+TILKLK+QYGEENAS+YQY SLM+KV+ L KKKD AA+EELSQ QV
Sbjct: 375 RFIYTNRRKAEAYDTILKLKKQYGEENASHYQYCSLMKKVEVLAKKKDIAALEELSQGQV 434

Query: 361 EKFMQQWNNNLDFRNDYKKRAIPLLNNRHLGVDGSMITDQKAEV 403
           +KFMQQWNN L+FRNDY++R +P L +R L +DG MIT QK+ +
Sbjct: 435 DKFMQQWNNCLEFRNDYRRRVVPSLYSRDLCLDGRMITKQKSSL 449

BLAST of CmaCh08G000030.1 vs. TrEMBL
Match: A0A0A0KFC5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G094690 PE=4 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 6.7e-82
Identity = 179/331 (54.08%), Postives = 239/331 (72.21%), Query Frame = 1

Query: 1   MEEGELYCKEKKVREEGSEVAGEVAILDYSDDERQLFDEMRVRGRHFYFAKVLPIENPKM 60
           M  GE     K V EE  + AG+  I+DY D   Q+FDEM V  R F F KVLP+ENP++
Sbjct: 75  MHGGEFDYGVKGVVEEEQKPAGDELIVDYYDYLHQIFDEMPVPVRQFSFVKVLPLENPEV 134

Query: 61  DAMIKKAEETIEKINRDQVVMAQKIRERMMDRDALRSKLNRMNF-GNYELALKWKRNSLD 120
            AMIKKAEETI K+N+DQV++AQKIR+R+MD D +R+KL  +N+  N  ++  W++  LD
Sbjct: 135 YAMIKKAEETIGKMNQDQVLLAQKIRKRVMDGDDVRAKLCMINYYDNCGISPNWEKERLD 194

Query: 121 FLYPSLDKLTFANNAYKGKSVNSCLSVGEIDKQKLCFLMVHGCKNMGDERKLLREVNAMQ 180
            L+ SLDKLTFAN+AYK KS+NSCLS GE+DKQKL F +VHG KNM DER LLRE+ A Q
Sbjct: 195 TLHLSLDKLTFANSAYKEKSINSCLSGGEVDKQKLSFSIVHGRKNMADERNLLREIKASQ 254

Query: 181 GEDGGMTLDELRAPIQHLQQRLSINY-VAHTEIDENARSEVILNAEKQHEIIRETALANA 240
           G+D GMT++EL APIQ L++++  NY       D+ AR++ IL   KQH+I RE A+A+A
Sbjct: 255 GKDDGMTVEELYAPIQRLKEQMCYNYRDCAKAADDAARNKTILKDIKQHQIAREKAIADA 314

Query: 241 VVNGKLWNSLGSKKSIQQQVQELNN---RSCELRERQREVNVKVRKVNKEVKMIEKDIRS 300
           VVNGKLWNSLGS+K+I+ ++QE ++    S ++    +++N+K+ K+ K+++ IEKDI S
Sbjct: 315 VVNGKLWNSLGSQKAIRAELQEEHSYEYNSGQVEHNHKKINIKIIKLKKKLEKIEKDISS 374

Query: 301 LQKLFTYANRKKDEAYNTILKLKRQYGEENA 327
           LQK     NRKK  AY+ ILKLK+QY EENA
Sbjct: 375 LQKRLKDTNRKKGAAYSIILKLKKQYEEENA 405

BLAST of CmaCh08G000030.1 vs. TrEMBL
Match: A0A072VCG6_MEDTR (Proton pump interactor, putative OS=Medicago truncatula GN=MTR_2g090520 PE=4 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 4.7e-35
Identity = 121/349 (34.67%), Postives = 182/349 (52.15%), Query Frame = 1

Query: 47  FYFAKVLPIENPKMDAMIKKAEETIEKINRDQVVMAQKIRERMMDRDALRSKLNRMNFGN 106
           FYF K+ P + P     IKK E  + K+N+D   + +KI E+M  R+ L S LNR+++  
Sbjct: 41  FYFVKLWPTD-PDSITKIKKEENVLMKLNQDICEVTEKITEKMSQREYLDSMLNRLHYLQ 100

Query: 107 YELA--LKWKRNSLDFLYPSLDKLTFANNAYKGKSVNSCLSVGEIDKQKLCFLMVHGCKN 166
            E    +  K   L  LY +LD+L F N A KG          + DK  +  +M+H CKN
Sbjct: 101 KERRNRVASKEKILRDLYMALDELNFMNKASKGGRFGE-----KPDKNSMNSVMLHSCKN 160

Query: 167 MGDERKLLREVNAMQ-GEDGGMTLDELRAPIQHLQQRLSINYVAHTEIDENARSEVILNA 226
           + +E+ +LR++N  Q G     +L  L+  I     R S  Y+ + +       +++   
Sbjct: 161 LAEEKNILRDINIQQKGVASFKSLKVLKKTI-----RWSF-YLKNWQ-------KLLREI 220

Query: 227 EKQHEIIRETALANAVVNGKLWNSLGSKKSIQQQVQELNNRSCELRERQREVNVKVRKVN 286
           E+      E A  N  V   + N    KK I+ Q++ L + S E R   +E   KVR   
Sbjct: 221 EQFQIQYMERASGNDPVKENISNYESLKKIIKDQIKLLFDESFENRREWKECGTKVRHGV 280

Query: 287 KEVKMIEKDIRSLQKLFTYANRKKDEAYNTILKLKRQYGEENASYYQYRSLMQKVQALVK 346
           KE++ I  ++ SL+   T  ++KK EAY  ILKLK  + EE   YYQ+ SL+ KV  L +
Sbjct: 281 KELEAINGELYSLKAKLTENHKKKGEAYQRILKLKNLHHEEILHYYQHCSLINKVHQLAE 340

Query: 347 KKDTAAIEELSQTQVEKFMQQWNNNLDFRNDYKKRAIPLLNNRHLGVDG 393
           +KD + ++E+S ++V KFM +WNNN  FR DY+K+    L  R L  DG
Sbjct: 341 EKDVSTLDEMSNSEVGKFMLEWNNNKTFREDYEKKVRQSLERRQLSRDG 370

BLAST of CmaCh08G000030.1 vs. TrEMBL
Match: A0A0R0HHC1_SOYBN (Uncharacterized protein (Fragment) OS=Glycine max GN=GLYMA_12G224800 PE=4 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 3.0e-29
Identity = 89/247 (36.03%), Postives = 143/247 (57.89%), Query Frame = 1

Query: 188 DELRAPIQHLQQRLSINYVAHTEIDENARSEVILNAEKQHEIIRETALANAVVNGKLWNS 247
           DEL + I  LQ R+    +  TE       + IL   KQ E  RE  +ANA +  KL  S
Sbjct: 162 DELNSVIYSLQYRIQHESIPLTE------EKQILREIKQLEGTREKVIANAAMRAKLQES 221

Query: 248 LGSKKSIQQQVQELNNRSCELRERQREVNVKVRKVNKEVKMIEKDIRSLQKLFTYANRKK 307
           +G K++IQ QV+ +      +++ +  +  K+++++  +K I+KDI+SLQ+  T  ++K+
Sbjct: 222 MGQKEAIQDQVKLIGGDLDGVKKEREAIRSKIKQIDDALKAIDKDIQSLQEELTAVSQKR 281

Query: 308 DEAYNTILKLKRQYGEENASYYQYRSLMQKVQALVKKKDTAAIEELSQTQVEKFMQQWNN 367
           D+A+ +I +L++Q  E N  +YQ R+++ K + L  KKD  A++E+SQT+VEKFM  WN 
Sbjct: 282 DKAFESIQQLRKQREEGNTYFYQSRTVLNKARELAAKKDINALDEVSQTEVEKFMALWNK 341

Query: 368 NLDFRNDYKKRAIPLLNNRHLGVDGSMITDQKAEVKDSKKAPKPEALSKARLKWLMKDPE 427
           +  FRNDY+KR +  L+ R L  DG M    +  + +  K  + EAL K   K   ++P+
Sbjct: 342 DKAFRNDYEKRILASLDMRQLSRDGRMRNPDEKPILEEPKPAEAEALPKMAAKQPKEEPK 401

Query: 428 -DPSELL 434
             P E L
Sbjct: 402 PSPQETL 402

BLAST of CmaCh08G000030.1 vs. TAIR10
Match: AT4G27500.1 (AT4G27500.1 proton pump interactor 1)

HSP 1 Score: 98.6 bits (244), Expect = 1.0e-20
Identity = 79/279 (28.32%), Postives = 143/279 (51.25%), Query Frame = 1

Query: 161 GCKNMGDERK-----LLREVNAMQGEDGGMTL--------DELRAPIQHLQQRLSINYVA 220
           G   M DE++     L + +  ++  DGG           +EL + I   Q R+    + 
Sbjct: 140 GFNTMFDEKRKEMEPLQQALGKLRSNDGGSARGPAICSSEEELNSMIYSYQYRIQHESIP 199

Query: 221 HTEIDENARSEVILNAEKQHEIIRETALANAVVNGKLWNSLGSKKSIQQQVQELNNRSCE 280
            TE       + IL   +  E  R+  +ANA +  K+  S+G K  IQ QV+ +      
Sbjct: 200 LTE------EKQILKEIRLLEGTRDKVIANAAMRAKIKESMGQKDDIQGQVKLMGAGLDG 259

Query: 281 LRERQREVNVKVRKVNKEVKMIEKDIRSLQKLFTYANRKKDEAYNTILKLKRQYGEENAS 340
           +++ ++ ++ ++ ++++++K  + +I  L+      + K+D+AY+ I  L+RQ  E N+ 
Sbjct: 260 VKKERQAISARINELSEKLKATKDEITVLENELKTVSEKRDKAYSNIHDLRRQRDETNSE 319

Query: 341 YYQYRSLMQKVQALVKKKDTAAIEELSQTQVEKFMQQWNNNLDFRNDYKKRAIPLLNNRH 400
           YYQ R+++ K + L  +K+ + +E L+  +VEKF+  W +  +FR DY+KR +  L++R 
Sbjct: 320 YYQNRTVLNKARDLAAQKNISELEALANAEVEKFISLWCSKKNFREDYEKRILQSLDSRQ 379

Query: 401 LGVDGSMIT-DQK----AEVKDSKKAP-KPEALSKARLK 421
           L  DG M   D+K     E   SK  P + E + KA+ K
Sbjct: 380 LSRDGRMRNPDEKPLIAPEAAPSKATPSETEVVPKAKAK 412

BLAST of CmaCh08G000030.1 vs. TAIR10
Match: AT3G15340.1 (AT3G15340.1 proton pump interactor 2)

HSP 1 Score: 70.1 bits (170), Expect = 3.8e-12
Identity = 53/205 (25.85%), Postives = 106/205 (51.71%), Query Frame = 1

Query: 205 YVAHTEIDENARSEVILNAEKQHEIIRETALANAVVNGKLWNSLGSKKSIQQQVQELNNR 264
           Y+AH +I+  +     +  E++  +++ET  A+ +V  +  +SL  K++   +V+ +   
Sbjct: 172 YIAHYQIEHGS-----IGFEEEDWVLKETEKADGIVLSE--DSLAEKEASINRVKSMAVE 231

Query: 265 SCELRERQREVNVKVRKVNKEVKMIEKDIRSLQKLFTYANRKKDEAYNTILKLKRQYGEE 324
             E+++    +  K+  ++ +V   + ++R L     +   ++D +Y  I  L+ Q  + 
Sbjct: 232 LNEVKKELDAITWKINHLSDKVGKSQNNLRVLDVKKAHILEERDRSYERIKMLRIQRDKG 291

Query: 325 NASYYQYRSLMQKVQALVKKKDTAAIEELSQTQVEKFMQQWNNNLDFRNDYKKRAIPLLN 384
            A++YQ   +M+K + L    +   +E  + ++ ++FM QWN++  FR+DY KR  P L 
Sbjct: 292 KAAFYQSLPVMRKARELAASGNVRDLEVFASSEADRFMTQWNDDKAFRDDYVKRISPSLC 351

Query: 385 NRHLGVDGSMITDQKAEVKDSKKAP 410
            R L  DG  I D + +V   KK P
Sbjct: 352 ERQLNQDG-RIKDPEVQVVWEKKVP 368

BLAST of CmaCh08G000030.1 vs. TAIR10
Match: AT1G53110.1 (AT1G53110.1 BEST Arabidopsis thaliana protein match is: proton pump interactor 2 (TAIR:AT3G15340.1))

HSP 1 Score: 68.9 bits (167), Expect = 8.6e-12
Identity = 41/125 (32.80%), Postives = 71/125 (56.80%), Query Frame = 1

Query: 267 ELRERQREVNVKVRKVNKEVKMIEKDIRSLQKLFTYANRKKDEAYNTILKLKRQYGEENA 326
           E+ + Q  V  ++ ++ + +  I+ +I  L         ++D+A   I  L+ Q  + NA
Sbjct: 114 EVEKEQEVVRGRIYRLGERLSEIKMEIELLDVQMACVLNQRDKAVERIKFLRMQRDKGNA 173

Query: 327 SYYQYRSLMQKVQALVKKKDTAAIEELSQTQVEKFMQQWNNNLDFRNDYKKRAIPLLNNR 386
           ++YQ R +M+K   L    +   +EEL+ ++VEKFM +WNN+  FR++YKKR +P +N R
Sbjct: 174 AFYQSRVVMKKAIELAASGNVRDLEELADSEVEKFMSRWNNDKAFRDNYKKRILPSVNER 233

Query: 387 HLGVD 392
            L  D
Sbjct: 234 KLRCD 238

BLAST of CmaCh08G000030.1 vs. TAIR10
Match: AT1G10880.1 (AT1G10880.1 Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein)

HSP 1 Score: 66.2 bits (160), Expect = 5.5e-11
Identity = 56/193 (29.02%), Postives = 92/193 (47.67%), Query Frame = 1

Query: 125 LDKLTFANNAYKGKSVNSCLSVGE--IDKQKLCFLMVHGCKNM-GDERKLLREVNAMQGE 184
           LDKLTFANNAY+ K      + GE  I+   L   M+H    M G ER+LL+ +N  +  
Sbjct: 451 LDKLTFANNAYRDKKAVISFASGEREINIHNLNHQMLHNAHCMDGGERRLLKMLNPNKDI 510

Query: 185 DGGMTLDELRAPIQHLQQRLSINYVAHTEIDENARSEVILNAEKQHEIIRETALANAVVN 244
           D G +L ++       Q+   +N  +   IDE   S      +++ E  R+    NA   
Sbjct: 511 DSGSSLAQVEE-----QKWPYLNTTSTITIDEETYSR----KQREFECERDQVFVNAPSK 570

Query: 245 GKLWNSLGSKKSIQQQVQELNNRSCELRERQREVNVKVRKVNKEVKMIEKDIRSLQKLFT 304
             LW SL S K ++ Q+Q +  +  + R++      K+    +++K  E +I+S++K   
Sbjct: 571 ASLWKSLPSTKDLRDQIQAMELKGEQKRKKVVGRRKKIESRERKIKKTENEIKSMRKTME 630

Query: 305 YANRKKDEAYNTI 315
               +  +A  TI
Sbjct: 631 RILNRMHKALETI 634

BLAST of CmaCh08G000030.1 vs. TAIR10
Match: AT1G20970.1 (AT1G20970.1 FUNCTIONS IN: molecular_function unknown)

HSP 1 Score: 59.7 bits (143), Expect = 5.2e-09
Identity = 60/245 (24.49%), Postives = 117/245 (47.76%), Query Frame = 1

Query: 165  MGDERKLLREVNAMQGEDGGMTLDELRAPIQHLQQRLSINYV-AHTEIDENARSEVILNA 224
            M +ER   + +++ + E     ++ L++ I  ++   S++ + +     E+      L+ 
Sbjct: 841  MAEERSARKAMHSKRQE-----IEALQSMISRVKSAASVDDIDSRVRNMEHTMQHTTLSL 900

Query: 225  EKQHEIIRETALANAVVNGKLWNSLGSKKSIQQQVQELNNRSCELRERQREVNV------ 284
             ++   +RE      +   ++ +S+G+K  ++Q + E       L+  ++E++       
Sbjct: 901  NEEKGFMREIKQLKQL-REQISSSMGTKDEVKQALDEKEKTEERLKVLRKELDALRNDLS 960

Query: 285  KVRKVNKEVKMI---EKDIRS-LQKLFTYANRKKDEAYNTILKLKRQYGEENASYYQYRS 344
            K  ++ K  K     E + +S LQ+ F  A+  + EA+  +  LK+Q  E+N  +++YR 
Sbjct: 961  KAEEITKAAKKKCDGEWEAQSKLQEQFRAADAVRQEAFVHLQDLKKQQREKNKYFFKYRD 1020

Query: 345  LMQKVQALVKKKDTAAIEELSQTQVEKFMQQWNNNLDFRNDYKK-------RAIPLLNNR 392
              +    +  KKD AA++ L   QVE FM  WNN+ +FR  Y K       R +  L+ R
Sbjct: 1021 NSRAASEMALKKDRAALQSLCSDQVENFMNMWNNDDEFRKYYVKSNTRSTFRRLGTLDGR 1079

BLAST of CmaCh08G000030.1 vs. NCBI nr
Match: gi|778711831|ref|XP_011656800.1| (PREDICTED: proton pump-interactor 1-like [Cucumis sativus])

HSP 1 Score: 553.5 bits (1425), Expect = 3.2e-154
Identity = 296/438 (67.58%), Postives = 352/438 (80.37%), Query Frame = 1

Query: 1   MEEGELY-CKEKKVREE--GSEVAGEVAILDYSDDERQLFDEMRVRGRHFYFAKVLPIEN 60
           MEEG+ + C+EK + EE  GSEV GE  I+D SDDE  +        RHFYF KVLP+EN
Sbjct: 1   MEEGDQFNCREKNMVEEQEGSEVIGEEGIIDCSDDEMPVGVV-----RHFYFVKVLPLEN 60

Query: 61  PKMDAMIKKAEETIEKINRDQVVMAQKIRERMMDRDALRSKLNRMN-FGNYELALKWKRN 120
           P +DAMIKKAEE I+K NRDQV++A KIRE+MMDRDA+RSKL+ M  +  YEL +KW + 
Sbjct: 61  PNLDAMIKKAEEMIDKTNRDQVLLATKIREKMMDRDAVRSKLSWMKKYDYYELTIKWHKE 120

Query: 121 SLDFLYPSLDKLTFANNAYKGKSVNSCLSVGEIDKQKLCFLMVHGCKNMGDERKLLREVN 180
            LD L+ SLDKLTFANNAYKGK VNSCLS GE+DKQKL FLM+HGCKNM DERKLLREVN
Sbjct: 121 RLDILHLSLDKLTFANNAYKGKPVNSCLSSGEVDKQKLHFLMLHGCKNMADERKLLREVN 180

Query: 181 AMQGEDGGMTLDELRAPIQHLQQRLSINYVAHTEIDENARSEVILNAEKQHEIIRETALA 240
           A QG+DGG+TLDEL APIQ L+ +   NY+  TE DENAR + IL   K HE+I E A+A
Sbjct: 181 ASQGKDGGITLDELHAPIQRLRGQFPTNYMGRTESDENARKQAILKGIKHHEVIMEKAIA 240

Query: 241 NAVVNGKLWNSLGSKKSIQQQVQELNNRSCELRERQREVNVKVRKVNKEVKMIEKDIRSL 300
           NAVVNGKLWNSL SKKSIQ++++ LNN S + RERQR+   ++RKVN E++ +EKDIRSL
Sbjct: 241 NAVVNGKLWNSLSSKKSIQEEIEMLNNSSIQPRERQRKTITEIRKVNLELEKVEKDIRSL 300

Query: 301 QKLFTYANRKKDEAYNTILKLKRQYGEENASYYQYRSLMQKVQALVKKKDTAAIEELSQT 360
           QKLFT ANRKKD+AY TIL+LK+QYGEENASYYQYRSLM+KV+ALVKKKD AA++E+SQT
Sbjct: 301 QKLFTDANRKKDDAYTTILRLKKQYGEENASYYQYRSLMKKVEALVKKKDIAAVQEVSQT 360

Query: 361 QVEKFMQQWNNNLDFRNDYKKRAIPLLNNRHLGVDGSM-ITDQKAEVKDSKKAPKPEALS 420
           QVEKFM QWN+N++FRNDYKKR  P L NRHLGVDG M + +QK EV+D++K  KP+ LS
Sbjct: 361 QVEKFMHQWNDNMEFRNDYKKRVNPSLKNRHLGVDGRMTMNNQKPEVEDTRKIIKPDTLS 420

Query: 421 KARLKWLMKDPEDPSELL 434
           K RLKWLMKD EDP ELL
Sbjct: 421 KTRLKWLMKDSEDPFELL 433

BLAST of CmaCh08G000030.1 vs. NCBI nr
Match: gi|700191246|gb|KGN46450.1| (hypothetical protein Csa_6G094680 [Cucumis sativus])

HSP 1 Score: 553.5 bits (1425), Expect = 3.2e-154
Identity = 296/438 (67.58%), Postives = 352/438 (80.37%), Query Frame = 1

Query: 1   MEEGELY-CKEKKVREE--GSEVAGEVAILDYSDDERQLFDEMRVRGRHFYFAKVLPIEN 60
           MEEG+ + C+EK + EE  GSEV GE  I+D SDDE  +        RHFYF KVLP+EN
Sbjct: 31  MEEGDQFNCREKNMVEEQEGSEVIGEEGIIDCSDDEMPVGVV-----RHFYFVKVLPLEN 90

Query: 61  PKMDAMIKKAEETIEKINRDQVVMAQKIRERMMDRDALRSKLNRMN-FGNYELALKWKRN 120
           P +DAMIKKAEE I+K NRDQV++A KIRE+MMDRDA+RSKL+ M  +  YEL +KW + 
Sbjct: 91  PNLDAMIKKAEEMIDKTNRDQVLLATKIREKMMDRDAVRSKLSWMKKYDYYELTIKWHKE 150

Query: 121 SLDFLYPSLDKLTFANNAYKGKSVNSCLSVGEIDKQKLCFLMVHGCKNMGDERKLLREVN 180
            LD L+ SLDKLTFANNAYKGK VNSCLS GE+DKQKL FLM+HGCKNM DERKLLREVN
Sbjct: 151 RLDILHLSLDKLTFANNAYKGKPVNSCLSSGEVDKQKLHFLMLHGCKNMADERKLLREVN 210

Query: 181 AMQGEDGGMTLDELRAPIQHLQQRLSINYVAHTEIDENARSEVILNAEKQHEIIRETALA 240
           A QG+DGG+TLDEL APIQ L+ +   NY+  TE DENAR + IL   K HE+I E A+A
Sbjct: 211 ASQGKDGGITLDELHAPIQRLRGQFPTNYMGRTESDENARKQAILKGIKHHEVIMEKAIA 270

Query: 241 NAVVNGKLWNSLGSKKSIQQQVQELNNRSCELRERQREVNVKVRKVNKEVKMIEKDIRSL 300
           NAVVNGKLWNSL SKKSIQ++++ LNN S + RERQR+   ++RKVN E++ +EKDIRSL
Sbjct: 271 NAVVNGKLWNSLSSKKSIQEEIEMLNNSSIQPRERQRKTITEIRKVNLELEKVEKDIRSL 330

Query: 301 QKLFTYANRKKDEAYNTILKLKRQYGEENASYYQYRSLMQKVQALVKKKDTAAIEELSQT 360
           QKLFT ANRKKD+AY TIL+LK+QYGEENASYYQYRSLM+KV+ALVKKKD AA++E+SQT
Sbjct: 331 QKLFTDANRKKDDAYTTILRLKKQYGEENASYYQYRSLMKKVEALVKKKDIAAVQEVSQT 390

Query: 361 QVEKFMQQWNNNLDFRNDYKKRAIPLLNNRHLGVDGSM-ITDQKAEVKDSKKAPKPEALS 420
           QVEKFM QWN+N++FRNDYKKR  P L NRHLGVDG M + +QK EV+D++K  KP+ LS
Sbjct: 391 QVEKFMHQWNDNMEFRNDYKKRVNPSLKNRHLGVDGRMTMNNQKPEVEDTRKIIKPDTLS 450

Query: 421 KARLKWLMKDPEDPSELL 434
           K RLKWLMKD EDP ELL
Sbjct: 451 KTRLKWLMKDSEDPFELL 463

BLAST of CmaCh08G000030.1 vs. NCBI nr
Match: gi|659119834|ref|XP_008459867.1| (PREDICTED: proton pump-interactor 2-like isoform X2 [Cucumis melo])

HSP 1 Score: 533.5 bits (1373), Expect = 3.5e-148
Identity = 289/436 (66.28%), Postives = 344/436 (78.90%), Query Frame = 1

Query: 1   MEEGELYCKEKKVREEGS--EVAGEVAILDYSDDERQLFDEMRVRGRHFYFAKVLPIENP 60
           MEEGE  C+EK + EEG   EV GE  I+D SDDE      M V  RHFYF KVLP+ENP
Sbjct: 1   MEEGEFNCREKNMVEEGEGPEVVGEEEIIDCSDDE------MPVGVRHFYFVKVLPLENP 60

Query: 61  KMDAMIKKAEETIEKINRDQVVMAQKIRERMMDRDALRSKLNRM-NFGNYELALKWKRNS 120
            +DAMIKKAEE IEK NRDQV++A KIRE+MMDRDA+RSKL  M N+  YEL +KW++  
Sbjct: 61  NLDAMIKKAEERIEKTNRDQVLLATKIREKMMDRDAVRSKLRWMRNYDYYELTIKWQKER 120

Query: 121 LDFLYPSLDKLTFANNAYKGKSVNSCLSVGEIDKQKLCFLMVHGCKNMGDERKLLREVNA 180
           LD L+  LDKLTFANNAYK K VNSCLS GE+DK+KL FLM+HGCKNM DERKLLREVN 
Sbjct: 121 LDILHLYLDKLTFANNAYKEKPVNSCLSSGEVDKRKLHFLMLHGCKNMADERKLLREVNE 180

Query: 181 MQGEDGGMTLDELRAPIQHLQQRLSINYVAHTEIDENARSEVILNAEKQHEIIRETALAN 240
            QG+DGG+TLDEL API+ L+Q+  + +    E DE+ R        KQHE+I E A+AN
Sbjct: 181 SQGKDGGITLDELHAPIRRLRQQFPMYF--RGESDEDPR--------KQHEVIIEKAIAN 240

Query: 241 AVVNGKLWNSLGSKKSIQQQVQELNNRSCELRERQREVNVKVRKVNKEVKMIEKDIRSLQ 300
           AVVNGKLWNSL SKKSIQ+++++L N S +LRERQR+ N ++RKV  E++ +EKDIRSLQ
Sbjct: 241 AVVNGKLWNSLSSKKSIQEEIEKLKNTSFQLRERQRKTNAEIRKVKLELEKVEKDIRSLQ 300

Query: 301 KLFTYANRKKDEAYNTILKLKRQYGEENASYYQYRSLMQKVQALVKKKDTAAIEELSQTQ 360
           KLFT ANRKK +AY TIL+LK+QYGEENASYY+YRSLM+KV+ALVKKKD   ++ELSQ Q
Sbjct: 301 KLFTDANRKKADAYKTILRLKKQYGEENASYYKYRSLMKKVEALVKKKDITEVQELSQKQ 360

Query: 361 VEKFMQQWNNNLDFRNDYKKRAIPLLNNRHLGVDGSMITDQKAEVKDSKKAPKPEALSKA 420
           V KFMQQWN+NL+FRNDYKKR  P L NRHLGVDG M+++QK EV+D++K  KPE LSKA
Sbjct: 361 VGKFMQQWNDNLEFRNDYKKRVNPSLTNRHLGVDGRMMSNQKPEVEDTRKVIKPETLSKA 420

Query: 421 RLKWLMKDPEDPSELL 434
           RLKWLMKD EDP ELL
Sbjct: 421 RLKWLMKDLEDPFELL 420

BLAST of CmaCh08G000030.1 vs. NCBI nr
Match: gi|659119830|ref|XP_008459865.1| (PREDICTED: proton pump-interactor 2-like isoform X1 [Cucumis melo])

HSP 1 Score: 519.6 bits (1337), Expect = 5.2e-144
Identity = 290/465 (62.37%), Postives = 344/465 (73.98%), Query Frame = 1

Query: 1   MEEGELYCKEKKVREEGS--EVAGEVAILDYSDDERQLFDEMRVRGRHFYFAKVLPIENP 60
           MEEGE  C+EK + EEG   EV GE  I+D SDDE      M V  RHFYF KVLP+ENP
Sbjct: 1   MEEGEFNCREKNMVEEGEGPEVVGEEEIIDCSDDE------MPVGVRHFYFVKVLPLENP 60

Query: 61  KMDAMIKKAEETIEKINRDQVVMAQKIRERMMDRDALRSKLNRM-NFGNYELALKWKRNS 120
            +DAMIKKAEE IEK NRDQV++A KIRE+MMDRDA+RSKL  M N+  YEL +KW++  
Sbjct: 61  NLDAMIKKAEERIEKTNRDQVLLATKIREKMMDRDAVRSKLRWMRNYDYYELTIKWQKER 120

Query: 121 LDFLYPSLDKLTFANNAYKGKSVNSCLSVGEIDK-------------------------- 180
           LD L+  LDKLTFANNAYK K VNSCLS GE+DK                          
Sbjct: 121 LDILHLYLDKLTFANNAYKEKPVNSCLSSGEVDKRVLSIELFLVHFCDALLLICLYVIDL 180

Query: 181 ---QKLCFLMVHGCKNMGDERKLLREVNAMQGEDGGMTLDELRAPIQHLQQRLSINYVAH 240
              QKL FLM+HGCKNM DERKLLREVN  QG+DGG+TLDEL API+ L+Q+  + +   
Sbjct: 181 SCEQKLHFLMLHGCKNMADERKLLREVNESQGKDGGITLDELHAPIRRLRQQFPMYF--R 240

Query: 241 TEIDENARSEVILNAEKQHEIIRETALANAVVNGKLWNSLGSKKSIQQQVQELNNRSCEL 300
            E DE+ R        KQHE+I E A+ANAVVNGKLWNSL SKKSIQ+++++L N S +L
Sbjct: 241 GESDEDPR--------KQHEVIIEKAIANAVVNGKLWNSLSSKKSIQEEIEKLKNTSFQL 300

Query: 301 RERQREVNVKVRKVNKEVKMIEKDIRSLQKLFTYANRKKDEAYNTILKLKRQYGEENASY 360
           RERQR+ N ++RKV  E++ +EKDIRSLQKLFT ANRKK +AY TIL+LK+QYGEENASY
Sbjct: 301 RERQRKTNAEIRKVKLELEKVEKDIRSLQKLFTDANRKKADAYKTILRLKKQYGEENASY 360

Query: 361 YQYRSLMQKVQALVKKKDTAAIEELSQTQVEKFMQQWNNNLDFRNDYKKRAIPLLNNRHL 420
           Y+YRSLM+KV+ALVKKKD   ++ELSQ QV KFMQQWN+NL+FRNDYKKR  P L NRHL
Sbjct: 361 YKYRSLMKKVEALVKKKDITEVQELSQKQVGKFMQQWNDNLEFRNDYKKRVNPSLTNRHL 420

Query: 421 GVDGSMITDQKAEVKDSKKAPKPEALSKARLKWLMKDPEDPSELL 434
           GVDG M+++QK EV+D++K  KPE LSKARLKWLMKD EDP ELL
Sbjct: 421 GVDGRMMSNQKPEVEDTRKVIKPETLSKARLKWLMKDLEDPFELL 449

BLAST of CmaCh08G000030.1 vs. NCBI nr
Match: gi|659119820|ref|XP_008459859.1| (PREDICTED: uncharacterized protein LOC103498858 isoform X1 [Cucumis melo])

HSP 1 Score: 349.0 bits (894), Expect = 1.2e-92
Identity = 205/398 (51.51%), Postives = 269/398 (67.59%), Query Frame = 1

Query: 40  MRVRGRHFYFAKVLPIENPKMDAMIKKAEETIEKINRDQVVMAQKIRERMMDRDALRSKL 99
           M V  R FYF KV  +EN   DA+IK  EETI K+N+DQ ++AQKIR+R MDRD + +KL
Sbjct: 1   MPVGVRQFYFVKVKALENSNTDAIIKP-EETISKMNQDQSLIAQKIRDRKMDRDRVIAKL 60

Query: 100 NRMNFGNY--ELALKWKRNSLDFLYPSLDKLTFANNAYKGKSVNSCLSVGEIDKQKLCFL 159
           N   +  +  +  L    + ++ L+ SL+K+T+ N A KGK   SCLS G++DKQ+L FL
Sbjct: 61  NDQQYYEFRDDFTLHCNTDRMNMLHFSLEKMTYVNKARKGKPNISCLSGGKVDKQELSFL 120

Query: 160 MVHGCKNMGDERKLLREVNAMQGEDGGMTLDELRAPIQHLQQRLSINYVAHTEIDENARS 219
           M HGCKNM DERK+LREV A Q  DGG T+DE+ + ++ L       +            
Sbjct: 121 MRHGCKNMADERKVLREVKARQENDGGSTVDEVDSSLESLFGTFGCPWYKPMGFQ----- 180

Query: 220 EVILNAEKQHEIIRETALANAVVNGKLWNSLGSKKSIQQQVQELNNRSCELRERQREVNV 279
             I++A K+ + I +       V G+LW+S   K  IQ+ +QEL + S  +R RQ     
Sbjct: 181 --IIHAIKKFQGIDDDP-----VEGELWSSSRIKNVIQEDLQELYDSSDYIRRRQARFRS 240

Query: 280 KVRKVNKEVKMIEKDIRSLQKLFTYANRKKDEAYNTILKLKRQYGEENASYYQYRSLMQK 339
           K +K  KE++ +EKDI +LQK   Y NR+K EAY+TILKLK+QYGEENAS+YQYRSLM+K
Sbjct: 241 KAKKTAKELEKVEKDISALQKRLIYTNRRKAEAYDTILKLKKQYGEENASHYQYRSLMKK 300

Query: 340 VQALVKKKDTAAIEELSQTQVEKFMQQWNNNLDFRNDYKKRAIPLLNNRHLGVDGSMITD 399
           V+ L KKKD AA+EELSQ QV+KFMQQWNN L+FRNDY++R +P L +R L +DG MIT+
Sbjct: 301 VEVLAKKKDVAALEELSQGQVDKFMQQWNNCLEFRNDYRQRVVPSLYSRDLCLDGRMITN 360

Query: 400 QKAE-VKDSKKAPKPEALSKARLKWLMKDPEDPSELLF 435
           QK+  V+D++K   PEALSK RLKWLMKD EDP ELLF
Sbjct: 361 QKSSLVEDTRKVIIPEALSKTRLKWLMKDAEDPFELLF 385

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PPI1_ARATH1.8e-1928.32Proton pump-interactor 1 OS=Arabidopsis thaliana GN=PPI1 PE=1 SV=2[more]
PPI2_ARATH6.8e-1125.85Proton pump-interactor 2 OS=Arabidopsis thaliana GN=PPI2 PE=2 SV=1[more]
PPI4_ARATH1.5e-1032.80Proton pump-interactor 4 OS=Arabidopsis thaliana GN=PPI4 PE=2 SV=1[more]
PPI3A_ARATH7.8e-0723.67Proton pump-interactor 3A OS=Arabidopsis thaliana GN=PPI3A PE=3 SV=1[more]
PPI3B_ARATH7.8e-0723.67Proton pump-interactor 3B OS=Arabidopsis thaliana GN=PPI3B PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K9L8_CUCSA2.3e-15467.58Uncharacterized protein OS=Cucumis sativus GN=Csa_6G094680 PE=4 SV=1[more]
A0A0A0KDK3_CUCSA1.8e-8748.76Uncharacterized protein OS=Cucumis sativus GN=Csa_6G094700 PE=4 SV=1[more]
A0A0A0KFC5_CUCSA6.7e-8254.08Uncharacterized protein OS=Cucumis sativus GN=Csa_6G094690 PE=4 SV=1[more]
A0A072VCG6_MEDTR4.7e-3534.67Proton pump interactor, putative OS=Medicago truncatula GN=MTR_2g090520 PE=4 SV=... [more]
A0A0R0HHC1_SOYBN3.0e-2936.03Uncharacterized protein (Fragment) OS=Glycine max GN=GLYMA_12G224800 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G27500.11.0e-2028.32 proton pump interactor 1[more]
AT3G15340.13.8e-1225.85 proton pump interactor 2[more]
AT1G53110.18.6e-1232.80 BEST Arabidopsis thaliana protein match is: proton pump interactor 2... [more]
AT1G10880.15.5e-1129.02 Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family p... [more]
AT1G20970.15.2e-0924.49 FUNCTIONS IN: molecular_function unknown[more]
Match NameE-valueIdentityDescription
gi|778711831|ref|XP_011656800.1|3.2e-15467.58PREDICTED: proton pump-interactor 1-like [Cucumis sativus][more]
gi|700191246|gb|KGN46450.1|3.2e-15467.58hypothetical protein Csa_6G094680 [Cucumis sativus][more]
gi|659119834|ref|XP_008459867.1|3.5e-14866.28PREDICTED: proton pump-interactor 2-like isoform X2 [Cucumis melo][more]
gi|659119830|ref|XP_008459865.1|5.2e-14462.37PREDICTED: proton pump-interactor 2-like isoform X1 [Cucumis melo][more]
gi|659119820|ref|XP_008459859.1|1.2e-9251.51PREDICTED: uncharacterized protein LOC103498858 isoform X1 [Cucumis melo][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
Vocabulary: Cellular Component
TermDefinition
GO:0005783endoplasmic reticulum
GO:0005886plasma membrane
Vocabulary: Biological Process
TermDefinition
GO:0010155regulation of proton transport
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010155 regulation of proton transport
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0005886 plasma membrane
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmaCh08G000030CmaCh08G000030gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmaCh08G000030.1CmaCh08G000030.1-proteinpolypeptide


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh08G000030.1.exon.8CmaCh08G000030.1.exon.8exon
CmaCh08G000030.1.exon.7CmaCh08G000030.1.exon.7exon
CmaCh08G000030.1.exon.6CmaCh08G000030.1.exon.6exon
CmaCh08G000030.1.exon.5CmaCh08G000030.1.exon.5exon
CmaCh08G000030.1.exon.4CmaCh08G000030.1.exon.4exon
CmaCh08G000030.1.exon.3CmaCh08G000030.1.exon.3exon
CmaCh08G000030.1.exon.2CmaCh08G000030.1.exon.2exon
CmaCh08G000030.1.exon.1CmaCh08G000030.1.exon.1exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh08G000030.1.five_prime_UTR.1CmaCh08G000030.1.five_prime_UTR.1five_prime_UTR
CmaCh08G000030.1.five_prime_UTR.2CmaCh08G000030.1.five_prime_UTR.2five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh08G000030.1.CDS.1CmaCh08G000030.1.CDS.1CDS
CmaCh08G000030.1.CDS.2CmaCh08G000030.1.CDS.2CDS
CmaCh08G000030.1.CDS.3CmaCh08G000030.1.CDS.3CDS
CmaCh08G000030.1.CDS.4CmaCh08G000030.1.CDS.4CDS
CmaCh08G000030.1.CDS.5CmaCh08G000030.1.CDS.5CDS
CmaCh08G000030.1.CDS.6CmaCh08G000030.1.CDS.6CDS
CmaCh08G000030.1.CDS.7CmaCh08G000030.1.CDS.7CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh08G000030.1.three_prime_UTR.1CmaCh08G000030.1.three_prime_UTR.1three_prime_UTR


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableunknownCoilCoilcoord: 279..299
scor
NoneNo IPR availablePANTHERPTHR32219FAMILY NOT NAMEDcoord: 47..130
score: 3.7E-43coord: 184..394
score: 3.7