CmaCh07G012120.1 (mRNA) Cucurbita maxima (Rimu)

NameCmaCh07G012120.1
TypemRNA
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionHeparanase-2, putative
LocationCma_Chr07 : 6662050 .. 6663846 (-)
Sequence length1005
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAACAAAATCGTATTGATTTTTGTTCTTGCCTTCATCCCGACCATTCTGGGTCGTAATGCTACAACGGGTACAATTGTGGTTGATGCAACCACAACAATAGCGGAAACTGATGAGAATTTCGTTTGTTTTACTTTGGACATTTGGCCCCATGACGAGTGTCGTTGGTCCAAACTTTGTGTTTGGGATGGTCATGCTTCTATGCTCAATTTGGTGAGAATCTTCGAAATCAAAACAAGAAGAAAATGCAACTCGTTGTATTTTATTAATCAACTCTTTCTTCTATTTTGTTCCAGGACTTAACTCTTCCTATCATGAACAAAGCCGTTCAAGGTTTGAGTATTACTAATGCATAAATGTTTCTTCTTTACGACATTCCGCTAAAAATCTAACCTAATTTGCCTTCTACCATTAGCTTTCAAGTCGGTAAGGATTAGAGTTGGAGGCACTTTACAAGACAAATTGATTTACAACGTTGGGGCTGGCTTCCAGGGAACCTGTCACCCATTTCAAGCAACCAAAGGTTCGTTGTTTGACTTCTCGGTGGGATGTTTGTACATGGAAAGGTGGGACGATTTGAACAACTTTTTCAACAATACCGGGTATGTTATTTTTCTTTTGTGTTTTTTTCTTACTTTCTATTTTCCGCTCCTGTACTAAATATATATATTTTAATATAACAGGGCGATTGTGACTTTTGGCTTAAACGCTCTCTTGGGTAAGTACAAGACGCAAGGAATTCAATGGGAAGGAAATTGGAACTATAGCAATGCCGAGGCTCTTATTCAATACACGGTAGAGAATAATTATCAAATAAATTCATGGGAGTTTGGTAAGTTAAATCATTCCATCACTCTTGGTTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGCAGTCCTCATATTTCTTATACATTTATCAATAGTTTTTGGTAATCAAATCTATTTAGACTTTTTTTCCCTCTATATTTGATTTGTCTCCACTTAAACATGAAGAAAAGAACAATGCTCGAGTACTTACAGAATGAATTGAATGCATTAGGTACTTGGATCAGCTCGGGATGGCTTCAATGTACAATACAAAAGTATATTGTCGACAGACTTTGATCGGTGGATTCTATGGTGTCATCAAATCCTCAACTTTACTTCCTACGCCAGATTACTATGGGTAAACCTTTCTAATTTATAAAAATTATATCACTTCTTCTCCCGTTTTCAAATCATCTAATCTTTAAACAAAGTTCGATATTTTCTTTTTTTTTTTATTAGTGCACTTCTCTTTCATCGACTCATGGGACCCCGTGTTCTCAAAATTGACAATAATGTCTCTTCTGACCTTCGTAGCTATGCCCATTGCTCGAGAGGAAGAGTAAGAAGTTGCTTTTAGATTTATATAAATTAAAACTTTATTTGATACCATTTATTGATCCTAATTTATCATTTTATGTAGTCTGGTGTAACCTTGCTTTTCATCAATTTGAGCAATGCAACCGACTTTATAATTGACCTTGAAAACAACATCAACTTGAGTTCAGGTAAGAGAAACGCTTCAAAAGGTCAAAGAGTGAAAATAGATTCACCAAGAGAGGAATACCATTTGACTCCGAAAGATGGTCTAGTTAGAAGTTCCACGGTGCTTTTAAATGGAAATCCATTGGAGACTACCAAAGAGGGAGACATACCAAATCTTATACCTGTCTACCGTCAGAGCAACTCTTCTATACATATTGCTGGTTGGTCCATTGCTTTCATTGTCGTCCCTCACTTTGTAGCTCCAGCATGCAACTAA

mRNA sequence

ATGGAAAACAAAATCGTATTGATTTTTGTTCTTGCCTTCATCCCGACCATTCTGGGTCGTAATGCTACAACGGGTACAATTGTGGTTGATGCAACCACAACAATAGCGGAAACTGATGAGAATTTCGTTTGTTTTACTTTGGACATTTGGCCCCATGACGAGTGTCGTTGGTCCAAACTTTGTGTTTGGGATGGTCATGCTTCTATGCTCAATTTGGACTTAACTCTTCCTATCATGAACAAAGCCGTTCAAGCTTTCAAGTCGGTAAGGATTAGAGTTGGAGGCACTTTACAAGACAAATTGATTTACAACGTTGGGGCTGGCTTCCAGGGAACCTGTCACCCATTTCAAGCAACCAAAGGTTCGTTGTTTGACTTCTCGGTGGGATGTTTGTACATGGAAAGGTGGGACGATTTGAACAACTTTTTCAACAATACCGGGTACTTGGATCAGCTCGGGATGGCTTCAATGTACAATACAAAAGTATATTGTCGACAGACTTTGATCGGTGGATTCTATGGTGTCATCAAATCCTCAACTTTACTTCCTACGCCAGATTACTATGGTGCACTTCTCTTTCATCGACTCATGGGACCCCGTGTTCTCAAAATTGACAATAATGTCTCTTCTGACCTTCGTAGCTATGCCCATTGCTCGAGAGGAAGATCTGGTGTAACCTTGCTTTTCATCAATTTGAGCAATGCAACCGACTTTATAATTGACCTTGAAAACAACATCAACTTGAGTTCAGGTAAGAGAAACGCTTCAAAAGGTCAAAGAGTGAAAATAGATTCACCAAGAGAGGAATACCATTTGACTCCGAAAGATGGTCTAGTTAGAAGTTCCACGGTGCTTTTAAATGGAAATCCATTGGAGACTACCAAAGAGGGAGACATACCAAATCTTATACCTGTCTACCGTCAGAGCAACTCTTCTATACATATTGCTGGTTGGTCCATTGCTTTCATTGTCGTCCCTCACTTTGTAGCTCCAGCATGCAACTAA

Coding sequence (CDS)

ATGGAAAACAAAATCGTATTGATTTTTGTTCTTGCCTTCATCCCGACCATTCTGGGTCGTAATGCTACAACGGGTACAATTGTGGTTGATGCAACCACAACAATAGCGGAAACTGATGAGAATTTCGTTTGTTTTACTTTGGACATTTGGCCCCATGACGAGTGTCGTTGGTCCAAACTTTGTGTTTGGGATGGTCATGCTTCTATGCTCAATTTGGACTTAACTCTTCCTATCATGAACAAAGCCGTTCAAGCTTTCAAGTCGGTAAGGATTAGAGTTGGAGGCACTTTACAAGACAAATTGATTTACAACGTTGGGGCTGGCTTCCAGGGAACCTGTCACCCATTTCAAGCAACCAAAGGTTCGTTGTTTGACTTCTCGGTGGGATGTTTGTACATGGAAAGGTGGGACGATTTGAACAACTTTTTCAACAATACCGGGTACTTGGATCAGCTCGGGATGGCTTCAATGTACAATACAAAAGTATATTGTCGACAGACTTTGATCGGTGGATTCTATGGTGTCATCAAATCCTCAACTTTACTTCCTACGCCAGATTACTATGGTGCACTTCTCTTTCATCGACTCATGGGACCCCGTGTTCTCAAAATTGACAATAATGTCTCTTCTGACCTTCGTAGCTATGCCCATTGCTCGAGAGGAAGATCTGGTGTAACCTTGCTTTTCATCAATTTGAGCAATGCAACCGACTTTATAATTGACCTTGAAAACAACATCAACTTGAGTTCAGGTAAGAGAAACGCTTCAAAAGGTCAAAGAGTGAAAATAGATTCACCAAGAGAGGAATACCATTTGACTCCGAAAGATGGTCTAGTTAGAAGTTCCACGGTGCTTTTAAATGGAAATCCATTGGAGACTACCAAAGAGGGAGACATACCAAATCTTATACCTGTCTACCGTCAGAGCAACTCTTCTATACATATTGCTGGTTGGTCCATTGCTTTCATTGTCGTCCCTCACTTTGTAGCTCCAGCATGCAACTAA

Protein sequence

MENKIVLIFVLAFIPTILGRNATTGTIVVDATTTIAETDENFVCFTLDIWPHDECRWSKLCVWDGHASMLNLDLTLPIMNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLFDFSVGCLYMERWDDLNNFFNNTGYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSSTLLPTPDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFIIDLENNINLSSGKRNASKGQRVKIDSPREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIPNLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN
BLAST of CmaCh07G012120.1 vs. Swiss-Prot
Match: HPSE1_ARATH (Heparanase-like protein 1 OS=Arabidopsis thaliana GN=At5g07830 PE=2 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 5.2e-51
Identity = 101/210 (48.10%), Postives = 140/210 (66.67%), Query Frame = 1

Query: 140 NNFFNNTGYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSSTLLPTPDYYGALLFHRLMGP 199
           + F ++  YLDQLGM++ +NTKVYCRQTL+GGFYG+++  T +P PDYY ALL+HRLMG 
Sbjct: 334 DTFIDSFWYLDQLGMSARHNTKVYCRQTLVGGFYGLLEKGTFVPNPDYYSALLWHRLMGK 393

Query: 200 RVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFIIDLENNINLSSGKRNASKGQ 259
            VL +  +    LR YAHCS+GR+GVTLL INLSN +DF + + N IN+     +  K  
Sbjct: 394 GVLAVQTDGPPQLRVYAHCSKGRAGVTLLLINLSNQSDFTVSVSNGINVVLNAESRKKKS 453

Query: 260 RV-KIDSP--------------REEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIPNLIP 319
            +  +  P              REEYHLTP++G++RS T++LNG  L+ T  GDIP+L P
Sbjct: 454 LLDTLKRPFSWIGSKASDGYLNREEYHLTPENGVLRSKTMVLNGKSLKPTATGDIPSLEP 513

Query: 320 VYRQSNSSIHIAGWSIAFIVVPHFVAPACN 335
           V R  NS +++   S++FIV+P+F A AC+
Sbjct: 514 VLRSVNSPLNVLPLSMSFIVLPNFDASACS 543

BLAST of CmaCh07G012120.1 vs. Swiss-Prot
Match: HPSE2_ARATH (Heparanase-like protein 2 OS=Arabidopsis thaliana GN=At5g61250 PE=2 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 3.5e-47
Identity = 97/208 (46.63%), Postives = 132/208 (63.46%), Query Frame = 1

Query: 142 FFNNTGYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSSTLLPTPDYYGALLFHRLMGPRV 201
           F N+  YLDQLG++S +NTKVYCRQ L+GGFYG+++  T +P PDYY ALL+HRLMG  +
Sbjct: 332 FINSFWYLDQLGISSKHNTKVYCRQALVGGFYGLLEKETFVPNPDYYSALLWHRLMGKGI 391

Query: 202 LKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFIIDLENNINL-----SSGKRNAS 261
           L +    S  LR+Y HCS+ R+G+T+L INLS  T F + + N + +     S  +++  
Sbjct: 392 LGVQTTASEYLRAYVHCSKRRAGITILLINLSKHTTFTVAVSNGVKVVLQAESMKRKSFL 451

Query: 262 KGQRVKID----------SPREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIPNLIPVY 321
           +  + K+             REEYHL+PKDG +RS  +LLNG PL  T  GDIP L PV 
Sbjct: 452 ETIKSKVSWVGNKASDGYLNREEYHLSPKDGDLRSKIMLLNGKPLVPTATGDIPKLEPVR 511

Query: 322 RQSNSSIHIAGWSIAFIVVPHFVAPACN 335
               S ++I   SI+FIV+P F APAC+
Sbjct: 512 HGVKSPVYINPLSISFIVLPTFDAPACS 539

BLAST of CmaCh07G012120.1 vs. Swiss-Prot
Match: HPSE3_ARATH (Heparanase-like protein 3 OS=Arabidopsis thaliana GN=At5g34940 PE=2 SV=2)

HSP 1 Score: 166.8 bits (421), Expect = 4.1e-40
Identity = 86/203 (42.36%), Postives = 124/203 (61.08%), Query Frame = 1

Query: 140 NNFFNNTGYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSSTLLPTPDYYGALLFHRLMGP 199
           N F  +  YLDQLGMAS+Y+TK YCRQ+LIGG YG++ ++   P PDYY AL++ +LMG 
Sbjct: 333 NAFVYSFWYLDQLGMASLYDTKTYCRQSLIGGNYGLLNTTNFTPNPDYYSALIWRQLMGR 392

Query: 200 RVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFI--IDLENNINLSSGK--RNA 259
           + L    + +  +RSY HC+R   G+T+L +NL N T  +  ++L N+ +L   K  ++ 
Sbjct: 393 KALFTTFSGTKKIRSYTHCARQSKGITVLLMNLDNTTTVVAKVELNNSFSLRHTKHMKSY 452

Query: 260 SKGQRVKIDSP-----REEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIPNLIPVYRQSN 319
            +        P     REEYHLT KDG + S T+LLNGN L+    GD+P + P++  S 
Sbjct: 453 KRASSQLFGGPNGVIQREEYHLTAKDGNLHSQTMLLNGNALQVNSMGDLPPIEPIHINST 512

Query: 320 SSIHIAGWSIAFIVVPHFVAPAC 334
             I IA +SI F+ + + V PAC
Sbjct: 513 EPITIAPYSIVFVHMRNVVVPAC 535

BLAST of CmaCh07G012120.1 vs. Swiss-Prot
Match: BAGLU_SCUBA (Baicalin-beta-D-glucuronidase OS=Scutellaria baicalensis GN=SGUS PE=1 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 9.2e-32
Identity = 71/187 (37.97%), Postives = 108/187 (57.75%), Query Frame = 1

Query: 140 NNFFNNTGYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSSTLLPTPDYYGALLFHRLMGP 199
           N F N   YL+ LG +++ +TK +CRQTL GG YG++++ T +P PDYY ALL+HRLMG 
Sbjct: 343 NTFINGFWYLNMLGYSALLDTKTFCRQTLTGGNYGLLQTGTYIPNPDYYSALLWHRLMGS 402

Query: 200 RVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFIIDLENNINLSSGKRNASKGQ 259
           +VLK +   + ++  YAHC++  +G+T+L +N    +   I L+ +              
Sbjct: 403 KVLKTEIVGTKNVYIYAHCAKKSNGITMLVLNHDGESSVKISLDPS-------------- 462

Query: 260 RVKIDSPREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIPNLIPVYRQSNSSIHIAGWS 319
             K  S REEYHLTP +  ++S  V LNG  L     G IP L PV + ++  + +A +S
Sbjct: 463 --KYGSKREEYHLTPVNNNLQSRLVKLNGELLHLDPSGVIPALNPVEKDNSKQLEVAPYS 513

Query: 320 IAFIVVP 327
             F+ +P
Sbjct: 523 FMFVHLP 513

BLAST of CmaCh07G012120.1 vs. Swiss-Prot
Match: HPSE_RAT (Heparanase OS=Rattus norvegicus GN=Hpse PE=2 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 2.1e-12
Identity = 59/196 (30.10%), Postives = 89/196 (45.41%), Query Frame = 1

Query: 148 YLDQLGMASMYNTKVYCRQTLIGGFYGVIKSSTLLPTPDYYGALLFHRLMGPRVL--KID 207
           +LD+LG+++    +V  RQ   G     +      P PDY+ +LLF +L+GP+VL  ++ 
Sbjct: 358 WLDKLGLSAQLGIEVVMRQVFFGAGNYHLVDENFEPLPDYWLSLLFKKLVGPKVLMSRVK 417

Query: 208 NNVSSDLRSYAHCS-----RGRSG-VTLLFINLSNATDFIIDLENNINLSSGKRNASKGQ 267
               S LR Y HC+     R R G +TL  +NL N T  +                 K  
Sbjct: 418 GPDRSKLRVYLHCTNVYHPRYREGDLTLYVLNLHNVTKHL-----------------KLP 477

Query: 268 RVKIDSPREEYHLTP--KDGLVRSSTVLLNGNPLETTKEGDIPNLIPVYRQSNSSIHIAG 327
                 P ++Y L P   DGL+ S +V LNG  L+   E  +P L      + SS+ +  
Sbjct: 478 PPMFSRPVDKYLLKPFGSDGLL-SKSVQLNGQTLKMVDEQTLPALTEKPLPAGSSLSVPA 535

Query: 328 WSIAFIVVPHFVAPAC 334
           +S  F V+ +    AC
Sbjct: 538 FSYGFFVIRNAKIAAC 535

BLAST of CmaCh07G012120.1 vs. TrEMBL
Match: A0A0A0KTJ9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G390000 PE=4 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.1e-71
Identity = 131/195 (67.18%), Postives = 157/195 (80.51%), Query Frame = 1

Query: 140 NNFFNNTGYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSSTLLPTPDYYGALLFHRLMGP 199
           + F N+  YLDQLGMA+ YNTKVYCRQTL+GG+YGV+++ T +PTPDYYGALLFHRLMG 
Sbjct: 345 DTFINSFWYLDQLGMAASYNTKVYCRQTLVGGYYGVLRTKTFIPTPDYYGALLFHRLMGS 404

Query: 200 RVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFIIDLENNINLSSGKRNASKGQ 259
            VLK+DNNVSS LR+YAHCSRGRSGVT+LFINLSN T+F I++EN++NLS  K       
Sbjct: 405 SVLKVDNNVSSYLRTYAHCSRGRSGVTMLFINLSNTTEFTINIENHMNLSLHKSKPKHSS 464

Query: 260 RVKIDSPREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIPNLIPVYRQSNSSIHIAGWS 319
              + + REEYHLTP++GL+RSSTVLLNG  LE T EG++P+L PVYR SNSSI I  WS
Sbjct: 465 SKNVGTQREEYHLTPQNGLLRSSTVLLNGKALELTNEGEVPDLTPVYRDSNSSISIPNWS 524

Query: 320 IAFIVVPHFVAPACN 335
           IAFIV+P FVA  CN
Sbjct: 525 IAFIVIPDFVAIGCN 539

BLAST of CmaCh07G012120.1 vs. TrEMBL
Match: A0A0A0KUF1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G043860 PE=4 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 5.5e-68
Identity = 127/198 (64.14%), Postives = 160/198 (80.81%), Query Frame = 1

Query: 140 NNFFNNTGYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSSTLLPTPDYYGALLFHRLMGP 199
           ++F N+  YLDQLGMA+ YNTKVYCRQTLIGGFY V+K+ TL+PTPDYYGALLFHRLMGP
Sbjct: 334 DSFINSFWYLDQLGMAAFYNTKVYCRQTLIGGFYSVLKAKTLVPTPDYYGALLFHRLMGP 393

Query: 200 RVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFIIDLENNINLSSGKRNASKGQ 259
            VLK+ N VS+ LR+YAHCSR RSG+++LFINLSN T+F I++++++ LS  KR   K  
Sbjct: 394 GVLKVHNKVSTYLRTYAHCSRERSGISMLFINLSNTTEFAINVKDHMTLSLHKRRKPKHG 453

Query: 260 RVKID---SPREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIPNLIPVYRQSNSSIHIA 319
              I+   +PREEYHLTP++GL+RSS VLLNG  L+ T EG++PNL P+Y+ SNSSI+IA
Sbjct: 454 SSSINNLGTPREEYHLTPQNGLLRSSNVLLNGKALQLTSEGELPNLTPIYKDSNSSINIA 513

Query: 320 GWSIAFIVVPHFVAPACN 335
            WSIAF+V+P FVA  CN
Sbjct: 514 TWSIAFVVIPDFVAIGCN 531

BLAST of CmaCh07G012120.1 vs. TrEMBL
Match: A0A0A0L5V7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G165650 PE=4 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 8.0e-67
Identity = 127/194 (65.46%), Postives = 153/194 (78.87%), Query Frame = 1

Query: 140 NNFFNNTGYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSSTLLPTPDYYGALLFHRLMGP 199
           N F +   Y+DQL MA++YNTKVYCRQTL+GGFYG++   TL P+PDYYGALLFHRLMG 
Sbjct: 331 NTFVDGFWYIDQLAMAALYNTKVYCRQTLVGGFYGILLPHTLAPSPDYYGALLFHRLMGS 390

Query: 200 RVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFIIDLENNINLSSGKRNASKGQ 259
            VLK+DNNVSS LR+YAHCS+ RSGVT+LFINLSN T+F +D+ENN+  +S    AS+  
Sbjct: 391 GVLKVDNNVSSYLRTYAHCSKERSGVTMLFINLSNETEFTVDIENNMMSTSLADKASQ-- 450

Query: 260 RVKIDSPREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIPNLIPVYRQSNSSIHIAGWS 319
                  REEYHL P +GLVRSSTVLLNGN LETT++GD+P+L P+YR SNSSI IA WS
Sbjct: 451 -------REEYHLIPNNGLVRSSTVLLNGNLLETTEDGDLPDLTPIYRDSNSSITIATWS 510

Query: 320 IAFIVVPHFVAPAC 334
           I F+V+PHF A AC
Sbjct: 511 IVFVVIPHFEASAC 515

BLAST of CmaCh07G012120.1 vs. TrEMBL
Match: A0A0A0LP82_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G011570 PE=4 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 9.2e-55
Identity = 115/213 (53.99%), Postives = 146/213 (68.54%), Query Frame = 1

Query: 140 NNFFNNTGYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSSTLLPTPDYYGALLFHRLMGP 199
           N F N+  YLDQLG+AS YNTKVYCRQTLIGG YG++ +STL+P PD+Y ALL+H+LMG 
Sbjct: 328 NTFINSFWYLDQLGLASKYNTKVYCRQTLIGGHYGLLNTSTLVPNPDFYSALLWHQLMGK 387

Query: 200 RVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFIIDLENNINL-----SSGKRN 259
            VL I  + SS LRSYAHCS+G +GVT+L INLSN T F I ++N+ N+      +G R 
Sbjct: 388 IVLPIGTDASSYLRSYAHCSKGNTGVTVLLINLSNQTQFSIHVQNSKNMFLDVQENGVRR 447

Query: 260 AS---KGQRVKI----------DSPREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIPN 319
                KG +  +             REEYHLTPKDG ++S T++LNG PLE T +GDIPN
Sbjct: 448 EKSFLKGMKKTVAWIGNKVSDATVSREEYHLTPKDGYLQSQTMVLNGTPLELTADGDIPN 507

Query: 320 LIPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN 335
           L P+ R  N+ IH+   SIAF+V P+F APAC+
Sbjct: 508 LNPILRDVNTPIHMDPLSIAFVVFPNFDAPACS 540

BLAST of CmaCh07G012120.1 vs. TrEMBL
Match: A0A151SLP9_CAJCA (Heparanase-like protein 1 OS=Cajanus cajan GN=KK1_001991 PE=4 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 3.5e-54
Identity = 112/195 (57.44%), Postives = 140/195 (71.79%), Query Frame = 1

Query: 140 NNFFNNTGYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSSTLLPTPDYYGALLFHRLMGP 199
           N F N+  YLDQLGMAS Y+TKVYCRQTLIGG YG++ ++T  P PDYY ALL+HRLMG 
Sbjct: 328 NTFLNSFWYLDQLGMASTYSTKVYCRQTLIGGNYGLLNTTTFTPNPDYYSALLWHRLMGK 387

Query: 200 RVLKIDNNVSSD-LRSYAHCSRGRSGVTLLFINLSNATDFIIDLENNINLSSGKRNASKG 259
           +VL + +++SS  LR+YAHCS+ R+GVTLL INLSN T FI+ + N +  S      +  
Sbjct: 388 KVLAVSSDISSPFLRTYAHCSKDRAGVTLLLINLSNQTHFILGVRNPVTASVENEVVTSI 447

Query: 260 QRVKIDSPREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIPNLIPVYRQSNSSIHIAGW 319
            + K+ + REEYHLTPKDG +RS T++LNG PLE T  GDIP L PV     S I++A  
Sbjct: 448 HKEKV-TFREEYHLTPKDGYLRSQTIVLNGIPLELTNSGDIPRLDPVQNNVQSPIYMAPL 507

Query: 320 SIAFIVVPHFVAPAC 334
           SIAFIV P+F APAC
Sbjct: 508 SIAFIVYPNFDAPAC 521

BLAST of CmaCh07G012120.1 vs. TAIR10
Match: AT5G07830.1 (AT5G07830.1 glucuronidase 2)

HSP 1 Score: 203.0 bits (515), Expect = 2.9e-52
Identity = 101/210 (48.10%), Postives = 140/210 (66.67%), Query Frame = 1

Query: 140 NNFFNNTGYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSSTLLPTPDYYGALLFHRLMGP 199
           + F ++  YLDQLGM++ +NTKVYCRQTL+GGFYG+++  T +P PDYY ALL+HRLMG 
Sbjct: 334 DTFIDSFWYLDQLGMSARHNTKVYCRQTLVGGFYGLLEKGTFVPNPDYYSALLWHRLMGK 393

Query: 200 RVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFIIDLENNINLSSGKRNASKGQ 259
            VL +  +    LR YAHCS+GR+GVTLL INLSN +DF + + N IN+     +  K  
Sbjct: 394 GVLAVQTDGPPQLRVYAHCSKGRAGVTLLLINLSNQSDFTVSVSNGINVVLNAESRKKKS 453

Query: 260 RV-KIDSP--------------REEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIPNLIP 319
            +  +  P              REEYHLTP++G++RS T++LNG  L+ T  GDIP+L P
Sbjct: 454 LLDTLKRPFSWIGSKASDGYLNREEYHLTPENGVLRSKTMVLNGKSLKPTATGDIPSLEP 513

Query: 320 VYRQSNSSIHIAGWSIAFIVVPHFVAPACN 335
           V R  NS +++   S++FIV+P+F A AC+
Sbjct: 514 VLRSVNSPLNVLPLSMSFIVLPNFDASACS 543

BLAST of CmaCh07G012120.1 vs. TAIR10
Match: AT5G61250.2 (AT5G61250.2 glucuronidase 1)

HSP 1 Score: 190.3 bits (482), Expect = 2.0e-48
Identity = 97/208 (46.63%), Postives = 132/208 (63.46%), Query Frame = 1

Query: 142 FFNNTGYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSSTLLPTPDYYGALLFHRLMGPRV 201
           F N+  YLDQLG++S +NTKVYCRQ L+GGFYG+++  T +P PDYY ALL+HRLMG  +
Sbjct: 332 FINSFWYLDQLGISSKHNTKVYCRQALVGGFYGLLEKETFVPNPDYYSALLWHRLMGKGI 391

Query: 202 LKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFIIDLENNINL-----SSGKRNAS 261
           L +    S  LR+Y HCS+ R+G+T+L INLS  T F + + N + +     S  +++  
Sbjct: 392 LGVQTTASEYLRAYVHCSKRRAGITILLINLSKHTTFTVAVSNGVKVVLQAESMKRKSFL 451

Query: 262 KGQRVKID----------SPREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIPNLIPVY 321
           +  + K+             REEYHL+PKDG +RS  +LLNG PL  T  GDIP L PV 
Sbjct: 452 ETIKSKVSWVGNKASDGYLNREEYHLSPKDGDLRSKIMLLNGKPLVPTATGDIPKLEPVR 511

Query: 322 RQSNSSIHIAGWSIAFIVVPHFVAPACN 335
               S ++I   SI+FIV+P F APAC+
Sbjct: 512 HGVKSPVYINPLSISFIVLPTFDAPACS 539

BLAST of CmaCh07G012120.1 vs. TAIR10
Match: AT5G34940.2 (AT5G34940.2 glucuronidase 3)

HSP 1 Score: 166.8 bits (421), Expect = 2.3e-41
Identity = 86/203 (42.36%), Postives = 124/203 (61.08%), Query Frame = 1

Query: 140 NNFFNNTGYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSSTLLPTPDYYGALLFHRLMGP 199
           N F  +  YLDQLGMAS+Y+TK YCRQ+LIGG YG++ ++   P PDYY AL++ +LMG 
Sbjct: 333 NAFVYSFWYLDQLGMASLYDTKTYCRQSLIGGNYGLLNTTNFTPNPDYYSALIWRQLMGR 392

Query: 200 RVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFI--IDLENNINLSSGK--RNA 259
           + L    + +  +RSY HC+R   G+T+L +NL N T  +  ++L N+ +L   K  ++ 
Sbjct: 393 KALFTTFSGTKKIRSYTHCARQSKGITVLLMNLDNTTTVVAKVELNNSFSLRHTKHMKSY 452

Query: 260 SKGQRVKIDSP-----REEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIPNLIPVYRQSN 319
            +        P     REEYHLT KDG + S T+LLNGN L+    GD+P + P++  S 
Sbjct: 453 KRASSQLFGGPNGVIQREEYHLTAKDGNLHSQTMLLNGNALQVNSMGDLPPIEPIHINST 512

Query: 320 SSIHIAGWSIAFIVVPHFVAPAC 334
             I IA +SI F+ + + V PAC
Sbjct: 513 EPITIAPYSIVFVHMRNVVVPAC 535

BLAST of CmaCh07G012120.1 vs. NCBI nr
Match: gi|449445228|ref|XP_004140375.1| (PREDICTED: heparanase-like protein 1 [Cucumis sativus])

HSP 1 Score: 278.5 bits (711), Expect = 1.5e-71
Identity = 131/195 (67.18%), Postives = 157/195 (80.51%), Query Frame = 1

Query: 140 NNFFNNTGYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSSTLLPTPDYYGALLFHRLMGP 199
           + F N+  YLDQLGMA+ YNTKVYCRQTL+GG+YGV+++ T +PTPDYYGALLFHRLMG 
Sbjct: 334 DTFINSFWYLDQLGMAASYNTKVYCRQTLVGGYYGVLRTKTFIPTPDYYGALLFHRLMGS 393

Query: 200 RVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFIIDLENNINLSSGKRNASKGQ 259
            VLK+DNNVSS LR+YAHCSRGRSGVT+LFINLSN T+F I++EN++NLS  K       
Sbjct: 394 SVLKVDNNVSSYLRTYAHCSRGRSGVTMLFINLSNTTEFTINIENHMNLSLHKSKPKHSS 453

Query: 260 RVKIDSPREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIPNLIPVYRQSNSSIHIAGWS 319
              + + REEYHLTP++GL+RSSTVLLNG  LE T EG++P+L PVYR SNSSI I  WS
Sbjct: 454 SKNVGTQREEYHLTPQNGLLRSSTVLLNGKALELTNEGEVPDLTPVYRDSNSSISIPNWS 513

Query: 320 IAFIVVPHFVAPACN 335
           IAFIV+P FVA  CN
Sbjct: 514 IAFIVIPDFVAIGCN 528

BLAST of CmaCh07G012120.1 vs. NCBI nr
Match: gi|700195824|gb|KGN51001.1| (hypothetical protein Csa_5G390000 [Cucumis sativus])

HSP 1 Score: 278.5 bits (711), Expect = 1.5e-71
Identity = 131/195 (67.18%), Postives = 157/195 (80.51%), Query Frame = 1

Query: 140 NNFFNNTGYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSSTLLPTPDYYGALLFHRLMGP 199
           + F N+  YLDQLGMA+ YNTKVYCRQTL+GG+YGV+++ T +PTPDYYGALLFHRLMG 
Sbjct: 345 DTFINSFWYLDQLGMAASYNTKVYCRQTLVGGYYGVLRTKTFIPTPDYYGALLFHRLMGS 404

Query: 200 RVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFIIDLENNINLSSGKRNASKGQ 259
            VLK+DNNVSS LR+YAHCSRGRSGVT+LFINLSN T+F I++EN++NLS  K       
Sbjct: 405 SVLKVDNNVSSYLRTYAHCSRGRSGVTMLFINLSNTTEFTINIENHMNLSLHKSKPKHSS 464

Query: 260 RVKIDSPREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIPNLIPVYRQSNSSIHIAGWS 319
              + + REEYHLTP++GL+RSSTVLLNG  LE T EG++P+L PVYR SNSSI I  WS
Sbjct: 465 SKNVGTQREEYHLTPQNGLLRSSTVLLNGKALELTNEGEVPDLTPVYRDSNSSISIPNWS 524

Query: 320 IAFIVVPHFVAPACN 335
           IAFIV+P FVA  CN
Sbjct: 525 IAFIVIPDFVAIGCN 539

BLAST of CmaCh07G012120.1 vs. NCBI nr
Match: gi|670412522|ref|XP_008647415.1| (PREDICTED: uncharacterized protein LOC100193781 isoform X2 [Zea mays])

HSP 1 Score: 266.2 bits (679), Expect = 7.9e-68
Identity = 147/345 (42.61%), Postives = 204/345 (59.13%), Query Frame = 1

Query: 6   VLIFVLAFIPTILGRNATTGTIVVDATTTIAETDENFVCFTLDIWPHDECRWSKLCVWDG 65
           +L+  L  +  +L R A      VD    IA T ENFVC TLD WP D+C +   C W G
Sbjct: 60  LLVGGLWLLAALLQRGAAAAVAAVDGRRAIAATGENFVCATLDWWPPDKCDYGT-CPW-G 119

Query: 66  HASMLNLDLTLPIMNKAVQAFKS-VRIRVGGTLQDKLIYNVGAGFQGTCHPFQATKGSLF 125
            A +LNLDL+  ++  AV+AF   + +R+GG+LQDK++Y   A     C PF      + 
Sbjct: 120 RAGLLNLDLSNKVLLNAVRAFSPPLMLRLGGSLQDKVVYGT-ADLGRPCAPFAKNASEMH 179

Query: 126 DFSVGCLYMERWDDLNNFFNNTGYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSSTLLPT 185
            F+ GCL + RWD+LN FF  +G+LDQLGM++ Y+TK YCRQ+LIGG YG++ ++T  P 
Sbjct: 180 GFTQGCLTLRRWDELNAFFQKSGFLDQLGMSAKYDTKSYCRQSLIGGNYGLLNTTTFQPN 239

Query: 186 PDYYGALLFHRLMGPRVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLS--NATDFIIDL 245
           PDYY ALL+HRLMG +VL    + ++ +R+YAHC+R   G+TLL INLS    T   + +
Sbjct: 240 PDYYSALLWHRLMGTKVLATTFSGTNKIRAYAHCARDSPGITLLLINLSGNTTTQVSVSV 299

Query: 246 ENNINLSSGKRNASK---GQRVK----------IDSPREEYHLTPKDGLVRSSTVLLNGN 305
                +++ K  A K   G++ +              R+EYHLTPKDG +RS  +LLNG 
Sbjct: 300 TTQGAVAAHKHGARKHVGGRKFRHVHVPSFDEAAGGVRDEYHLTPKDGNLRSQIMLLNGR 359

Query: 306 PLETTKEGDIPNLIPVYRQSNSSIHIAGWSIAFIVVPHFVAPACN 335
            L T   G+IP L  V   +   I +A +SI F  + HF APAC+
Sbjct: 360 ALATDTAGNIPALEAVKMDAAQPIAVAPYSIVFARISHFNAPACS 401

BLAST of CmaCh07G012120.1 vs. NCBI nr
Match: gi|449457614|ref|XP_004146543.1| (PREDICTED: heparanase-like protein 1 [Cucumis sativus])

HSP 1 Score: 266.2 bits (679), Expect = 7.9e-68
Identity = 127/198 (64.14%), Postives = 160/198 (80.81%), Query Frame = 1

Query: 140 NNFFNNTGYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSSTLLPTPDYYGALLFHRLMGP 199
           ++F N+  YLDQLGMA+ YNTKVYCRQTLIGGFY V+K+ TL+PTPDYYGALLFHRLMGP
Sbjct: 267 DSFINSFWYLDQLGMAAFYNTKVYCRQTLIGGFYSVLKAKTLVPTPDYYGALLFHRLMGP 326

Query: 200 RVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFIIDLENNINLSSGKRNASKGQ 259
            VLK+ N VS+ LR+YAHCSR RSG+++LFINLSN T+F I++++++ LS  KR   K  
Sbjct: 327 GVLKVHNKVSTYLRTYAHCSRERSGISMLFINLSNTTEFAINVKDHMTLSLHKRRKPKHG 386

Query: 260 RVKID---SPREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIPNLIPVYRQSNSSIHIA 319
              I+   +PREEYHLTP++GL+RSS VLLNG  L+ T EG++PNL P+Y+ SNSSI+IA
Sbjct: 387 SSSINNLGTPREEYHLTPQNGLLRSSNVLLNGKALQLTSEGELPNLTPIYKDSNSSINIA 446

Query: 320 GWSIAFIVVPHFVAPACN 335
            WSIAF+V+P FVA  CN
Sbjct: 447 TWSIAFVVIPDFVAIGCN 464

BLAST of CmaCh07G012120.1 vs. NCBI nr
Match: gi|700198112|gb|KGN53270.1| (hypothetical protein Csa_4G043860 [Cucumis sativus])

HSP 1 Score: 266.2 bits (679), Expect = 7.9e-68
Identity = 127/198 (64.14%), Postives = 160/198 (80.81%), Query Frame = 1

Query: 140 NNFFNNTGYLDQLGMASMYNTKVYCRQTLIGGFYGVIKSSTLLPTPDYYGALLFHRLMGP 199
           ++F N+  YLDQLGMA+ YNTKVYCRQTLIGGFY V+K+ TL+PTPDYYGALLFHRLMGP
Sbjct: 334 DSFINSFWYLDQLGMAAFYNTKVYCRQTLIGGFYSVLKAKTLVPTPDYYGALLFHRLMGP 393

Query: 200 RVLKIDNNVSSDLRSYAHCSRGRSGVTLLFINLSNATDFIIDLENNINLSSGKRNASKGQ 259
            VLK+ N VS+ LR+YAHCSR RSG+++LFINLSN T+F I++++++ LS  KR   K  
Sbjct: 394 GVLKVHNKVSTYLRTYAHCSRERSGISMLFINLSNTTEFAINVKDHMTLSLHKRRKPKHG 453

Query: 260 RVKID---SPREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIPNLIPVYRQSNSSIHIA 319
              I+   +PREEYHLTP++GL+RSS VLLNG  L+ T EG++PNL P+Y+ SNSSI+IA
Sbjct: 454 SSSINNLGTPREEYHLTPQNGLLRSSNVLLNGKALQLTSEGELPNLTPIYKDSNSSINIA 513

Query: 320 GWSIAFIVVPHFVAPACN 335
            WSIAF+V+P FVA  CN
Sbjct: 514 TWSIAFVVIPDFVAIGCN 531

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
HPSE1_ARATH5.2e-5148.10Heparanase-like protein 1 OS=Arabidopsis thaliana GN=At5g07830 PE=2 SV=1[more]
HPSE2_ARATH3.5e-4746.63Heparanase-like protein 2 OS=Arabidopsis thaliana GN=At5g61250 PE=2 SV=1[more]
HPSE3_ARATH4.1e-4042.36Heparanase-like protein 3 OS=Arabidopsis thaliana GN=At5g34940 PE=2 SV=2[more]
BAGLU_SCUBA9.2e-3237.97Baicalin-beta-D-glucuronidase OS=Scutellaria baicalensis GN=SGUS PE=1 SV=1[more]
HPSE_RAT2.1e-1230.10Heparanase OS=Rattus norvegicus GN=Hpse PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KTJ9_CUCSA1.1e-7167.18Uncharacterized protein OS=Cucumis sativus GN=Csa_5G390000 PE=4 SV=1[more]
A0A0A0KUF1_CUCSA5.5e-6864.14Uncharacterized protein OS=Cucumis sativus GN=Csa_4G043860 PE=4 SV=1[more]
A0A0A0L5V7_CUCSA8.0e-6765.46Uncharacterized protein OS=Cucumis sativus GN=Csa_3G165650 PE=4 SV=1[more]
A0A0A0LP82_CUCSA9.2e-5553.99Uncharacterized protein OS=Cucumis sativus GN=Csa_1G011570 PE=4 SV=1[more]
A0A151SLP9_CAJCA3.5e-5457.44Heparanase-like protein 1 OS=Cajanus cajan GN=KK1_001991 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G07830.12.9e-5248.10 glucuronidase 2[more]
AT5G61250.22.0e-4846.63 glucuronidase 1[more]
AT5G34940.22.3e-4142.36 glucuronidase 3[more]
Match NameE-valueIdentityDescription
gi|449445228|ref|XP_004140375.1|1.5e-7167.18PREDICTED: heparanase-like protein 1 [Cucumis sativus][more]
gi|700195824|gb|KGN51001.1|1.5e-7167.18hypothetical protein Csa_5G390000 [Cucumis sativus][more]
gi|670412522|ref|XP_008647415.1|7.9e-6842.61PREDICTED: uncharacterized protein LOC100193781 isoform X2 [Zea mays][more]
gi|449457614|ref|XP_004146543.1|7.9e-6864.14PREDICTED: heparanase-like protein 1 [Cucumis sativus][more]
gi|700198112|gb|KGN53270.1|7.9e-6864.14hypothetical protein Csa_4G043860 [Cucumis sativus][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR005199Glyco_hydro_79
Vocabulary: Molecular Function
TermDefinition
GO:0016798hydrolase activity, acting on glycosyl bonds
Vocabulary: Cellular Component
TermDefinition
GO:0016020membrane
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
cellular_component GO:0016020 membrane
molecular_function GO:0016798 hydrolase activity, acting on glycosyl bonds

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmaCh07G012120CmaCh07G012120gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmaCh07G012120.1CmaCh07G012120.1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh07G012120.1.CDS.6CmaCh07G012120.1.CDS.6CDS
CmaCh07G012120.1.CDS.5CmaCh07G012120.1.CDS.5CDS
CmaCh07G012120.1.CDS.4CmaCh07G012120.1.CDS.4CDS
CmaCh07G012120.1.CDS.3CmaCh07G012120.1.CDS.3CDS
CmaCh07G012120.1.CDS.2CmaCh07G012120.1.CDS.2CDS
CmaCh07G012120.1.CDS.1CmaCh07G012120.1.CDS.1CDS


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh07G012120.1.exon.6CmaCh07G012120.1.exon.6exon
CmaCh07G012120.1.exon.5CmaCh07G012120.1.exon.5exon
CmaCh07G012120.1.exon.4CmaCh07G012120.1.exon.4exon
CmaCh07G012120.1.exon.3CmaCh07G012120.1.exon.3exon
CmaCh07G012120.1.exon.2CmaCh07G012120.1.exon.2exon
CmaCh07G012120.1.exon.1CmaCh07G012120.1.exon.1exon


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005199Glycoside hydrolase, family 79PANTHERPTHR14363HEPARANASE-RELATEDcoord: 140..334
score: 7.2
IPR005199Glycoside hydrolase, family 79PFAMPF03662Glyco_hydro_79ncoord: 25..168
score: 1.2
NoneNo IPR availablePANTHERPTHR14363:SF17HEPARANASE-LIKE PROTEIN 1-RELATEDcoord: 140..334
score: 7.2