CmaCh07G002990.1 (mRNA) Cucurbita maxima (Rimu)

BLAST of CmaCh07G002990.1 vs. Swiss-Prot
Match: PME1_FICPW (Pectinesterase/pectinesterase inhibitor OS=Ficus pumila var. awkeotsang PE=1 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 9.7e-169
Identity = 307/535 (57.38%), Postives = 383/535 (71.59%), Query Frame = 1

Query: 166 ILFFSLLLGSAIFITNKLINSNPSENDHHPFLHNICYKAYNQSSCIQMLASEFPLLQETK 225
           I FF L++  A  ++     S+P  + HH     IC ++ N+ SC+ M++    L     
Sbjct: 19  ITFFLLVISFAALVSTGF--SSPELSLHH----KICDQSVNKESCLAMISEVTGL----N 78

Query: 226 TTNAANFLQSFLRKSTSKIVNTIESAKDLRRRINNPRGEAALADCVELMEISMDRITDSI 285
             +  N L+SFL K+T +I    E+A D  RRINNP+   AL DC ELM++S +R+ DSI
Sbjct: 79  MADHRNLLKSFLEKTTPRIQKAFETANDASRRINNPQERTALLDCAELMDLSKERVVDSI 138

Query: 286 LALERRRCGTI--ENAHTWLSSVLTNHVACSDEIENSISK--AAAMDLGVEELIVIGRNS 345
             L  +   T   E+ H WLS VLTNHV C D +E   +      M+  + ELI+  R S
Sbjct: 139 SILFHQNLTTRSHEDLHVWLSGVLTNHVTCLDGLEEGSTDYIKTLMESHLNELILRARTS 198

Query: 346 LGMLVSIWGSGKKE----GMGYPSWLKKGDRRLLEVVGRGMKADIVVAKDGSGNFKTVKE 405
           L + V+++ +           +P+W+  GDRRLL+ +G+ ++ DIVVAKDGSG+++T+ E
Sbjct: 199 LAIFVTLFPAKSNVIEPVTGNFPTWVTAGDRRLLQTLGKDIEPDIVVAKDGSGDYETLNE 258

Query: 406 AVEAVPDKSKSRIVIYVKKGIYKENVEVGKNKKNVMLVGDGMDSTIITASLNVVDGSTTF 465
           AV A+PD SK R+++ V+ GIY+ENV+ G  KKNVMLVG+GMD TIIT S NVVDGSTTF
Sbjct: 259 AVAAIPDNSKKRVIVLVRTGIYEENVDFGYQKKNVMLVGEGMDYTIITGSRNVVDGSTTF 318

Query: 466 KSATVAAVGDGFIAQDIWFRNTAEPEKHQAVALRVGADQSVINRCRIDAYQDTLYAHTNR 525
            SATVAAVGDGFIAQDI F+NTA PEK+QAVALR+GAD++VINRCRIDAYQDTLY H  R
Sbjct: 319 DSATVAAVGDGFIAQDICFQNTAGPEKYQAVALRIGADETVINRCRIDAYQDTLYPHNYR 378

Query: 526 QFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQANMVTAQGRTDPNQNTGTSIQRC 585
           QFYRD  ITGTVDFIFGNAAVV QNC + PR+ M  Q N +TAQGRTDPNQNTGTSIQ C
Sbjct: 379 QFYRDRNITGTVDFIFGNAAVVFQNCNLIPRKQMKGQENTITAQGRTDPNQNTGTSIQNC 438

Query: 586 EIVASPDLEPVKKSIKTYLGRPWKEYSRTVVMQSTLGDLIDPAGWAEWNGEFALKTLYYG 645
           EI AS DLEPV+ + K+YLGRPWKEYSRTVVM+S + D+IDPAGW EW+ +FALKTL+YG
Sbjct: 439 EIFASADLEPVEDTFKSYLGRPWKEYSRTVVMESYISDVIDPAGWLEWDRDFALKTLFYG 498

Query: 646 EYSNNGPGSNVSKRVKWPGYHVITSPAVAQKFTVDSLIQGAEWLGSSGVTFIPGL 693
           EY N GPGS  S+RVKWPGYHVITSP VA++FTV  LIQG  WLGS+GV +  GL
Sbjct: 499 EYRNGGPGSGTSERVKWPGYHVITSPEVAEQFTVAELIQGGSWLGSTGVDYTAGL 543

BLAST of CmaCh07G002990.1 vs. Swiss-Prot
Match: PME21_SOLLC (Pectinesterase 2.1 OS=Solanum lycopersicum GN=PME2.1 PE=2 SV=2)

HSP 1 Score: 572.8 bits (1475), Expect = 5.1e-162
Identity = 298/533 (55.91%), Postives = 377/533 (70.73%), Query Frame = 1

Query: 164 FTILFFSLLLGSAIFITNKLINSNPSENDHHPFLHNICYKAYNQSSCIQMLASEFPLLQE 223
           F IL F + L  A+F+   L+     E  H     N+C  A +   C+  ++        
Sbjct: 21  FKILTFVVTLFVALFLVVFLVAPYQFEIKHS----NLCKTAQDSQLCLSYVSDLISNEIV 80

Query: 224 TKTTNAANFLQSFLRKSTSKIVNTIESAKDLRRRINNPRGEAALADCVELMEISMDRITD 283
           T  ++  + L+ FL  S  ++ N I   + ++ +IN+ R + AL DC+EL+++S+D + D
Sbjct: 81  TSDSDGLSILKKFLVYSVHQMNNAIPVVRKIKNQINDIREQGALTDCLELLDLSVDLVCD 140

Query: 284 SILALERRRCGTIENAHTWLSSVLTNHVACSDEIENSISKAAAMDLGVEELIVIGRNSLG 343
           SI A+++R      NA +WLS VLTNHV C DE++ S +KA      ++ELI   + +L 
Sbjct: 141 SIAAIDKRSRSEHANAQSWLSGVLTNHVTCLDELD-SFTKAMINGTNLDELISRAKVALA 200

Query: 344 MLVSIWGSGK---KEGMG-YPSWLKKGDRRLLEVVGRGMKADIVVAKDGSGNFKTVKEAV 403
           ML S+        + G+G  PSW+   DR+L+E  G+ + A+ VVAKDG+G ++T+ EAV
Sbjct: 201 MLASVTTPNDEVLRPGLGKMPSWVSSRDRKLMESSGKDIGANAVVAKDGTGKYRTLAEAV 260

Query: 404 EAVPDKSKSRIVIYVKKGIYKENVEVGKNKKNVMLVGDGMDSTIITASLNVVDGSTTFKS 463
            A PDKSK+R VIYVK+G YKENVEV   K N+M++GDGM +TIIT SLNVVDGSTTF S
Sbjct: 261 AAAPDKSKTRYVIYVKRGTYKENVEVSSRKMNLMIIGDGMYATIITGSLNVVDGSTTFHS 320

Query: 464 ATVAAVGDGFIAQDIWFRNTAEPEKHQAVALRVGADQSVINRCRIDAYQDTLYAHTNRQF 523
           AT+AAVG GFI QDI  +NTA P KHQAVALRVGAD+SVINRCRIDAYQDTLYAH+ RQF
Sbjct: 321 ATLAAVGKGFILQDICIQNTAGPAKHQAVALRVGADKSVINRCRIDAYQDTLYAHSQRQF 380

Query: 524 YRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQANMVTAQGRTDPNQNTGTSIQRCEI 583
           YRDS +TGT+DFIFGNAAVV Q C++  R+P   Q NMVTAQGRTDPNQ TGTSIQ C+I
Sbjct: 381 YRDSYVTGTIDFIFGNAAVVFQKCQLVARKPGKYQQNMVTAQGRTDPNQATGTSIQFCDI 440

Query: 584 VASPDLEPVKKSIKTYLGRPWKEYSRTVVMQSTLGDLIDPAGWAEWNGEFALKTLYYGEY 643
           +ASPDL+PV K   TYLGRPWK+YSRTVVM+S LG LIDP+GWAEW+G+FALKTLYYGE+
Sbjct: 441 IASPDLKPVVKEFPTYLGRPWKKYSRTVVMESYLGGLIDPSGWAEWHGDFALKTLYYGEF 500

Query: 644 SNNGPGSNVSKRVKWPGYHVITSPAVAQKFTVDSLIQGAEWLGSSGVTFIPGL 693
            NNGPG+  SKRVKWPGYHVIT PA A  FTV  LIQG  WL S+ V ++ GL
Sbjct: 501 MNNGPGAGTSKRVKWPGYHVITDPAEAMSFTVAKLIQGGSWLRSTDVAYVDGL 548

BLAST of CmaCh07G002990.1 vs. Swiss-Prot
Match: PME22_SOLLC (Pectinesterase 2.2 OS=Solanum lycopersicum GN=PME2.2 PE=3 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 1.3e-160
Identity = 299/533 (56.10%), Postives = 372/533 (69.79%), Query Frame = 1

Query: 164 FTILFFSLLLGSAIFITNKLINSNPSENDHHPFLHNICYKAYNQSSCIQMLASEFPLLQE 223
           F IL F + L  A+F+   L+     E  H     N+C  A +   C+  ++        
Sbjct: 21  FKILTFVVTLFVALFLVVFLVAPYQFEIKHS----NLCKTAQDSQLCLSYVSDLMSNEIV 80

Query: 224 TKTTNAANFLQSFLRKSTSKIVNTIESAKDLRRRINNPRGEAALADCVELMEISMDRITD 283
           T  ++  + L  FL     ++ N I     ++ +IN+ R E AL DC+EL++ S+D ++D
Sbjct: 81  TTDSDGLSILMKFLVNYVHQMNNAIPVVSKMKNQINDIRQEGALTDCLELLDQSVDLVSD 140

Query: 284 SILALERRRCGTIENAHTWLSSVLTNHVACSDEIENSISKAAAMDLGVEELIVIGRNSLG 343
           SI A+++R      NA +WLS VLTNHV C DE++ S +KA      ++ELI   + +L 
Sbjct: 141 SIAAIDKRTHSEHANAQSWLSGVLTNHVTCLDELD-SFTKAMINGTNLDELISRAKVALA 200

Query: 344 MLVSIWGSGK---KEGMG-YPSWLKKGDRRLLEVVGRGMKADIVVAKDGSGNFKTVKEAV 403
           ML S+        + G+G  PSW+   DR+L+E  G+ + A+ VVAKDG+G ++T+ EAV
Sbjct: 201 MLASVTTPNDDVLRPGLGKMPSWVSSRDRKLMESSGKDIGANAVVAKDGTGKYRTLAEAV 260

Query: 404 EAVPDKSKSRIVIYVKKGIYKENVEVGKNKKNVMLVGDGMDSTIITASLNVVDGSTTFKS 463
            A PDKSK+R VIYVK+GIYKENVEV   K  +M+VGDGM +TIIT +LNVVDGSTTF S
Sbjct: 261 AAAPDKSKTRYVIYVKRGIYKENVEVSSRKMKLMIVGDGMHATIITGNLNVVDGSTTFHS 320

Query: 464 ATVAAVGDGFIAQDIWFRNTAEPEKHQAVALRVGADQSVINRCRIDAYQDTLYAHTNRQF 523
           AT+AAVG GFI QDI  +NTA P KHQAVALRVGAD+SVINRCRIDAYQDTLYAH+ RQF
Sbjct: 321 ATLAAVGKGFILQDICIQNTAGPAKHQAVALRVGADKSVINRCRIDAYQDTLYAHSQRQF 380

Query: 524 YRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQANMVTAQGRTDPNQNTGTSIQRCEI 583
           YRDS +TGT+DFIFGNAAVV Q CK+  R+P   Q NMVTAQGRTDPNQ TGTSIQ C I
Sbjct: 381 YRDSYVTGTIDFIFGNAAVVFQKCKLVARKPGKYQQNMVTAQGRTDPNQATGTSIQFCNI 440

Query: 584 VASPDLEPVKKSIKTYLGRPWKEYSRTVVMQSTLGDLIDPAGWAEWNGEFALKTLYYGEY 643
           +AS DLEPV K   TYLGRPWK+YSRTVVM+S LG LI+PAGWAEW+G+FALKTLYYGE+
Sbjct: 441 IASSDLEPVLKEFPTYLGRPWKKYSRTVVMESYLGGLINPAGWAEWDGDFALKTLYYGEF 500

Query: 644 SNNGPGSNVSKRVKWPGYHVITSPAVAQKFTVDSLIQGAEWLGSSGVTFIPGL 693
            NNGPG+  SKRVKWPGYH IT PA A  FTV  LIQG  WL S+GV ++ GL
Sbjct: 501 MNNGPGAGTSKRVKWPGYHCITDPAEAMPFTVAKLIQGGSWLRSTGVAYVDGL 548

BLAST of CmaCh07G002990.1 vs. Swiss-Prot
Match: PME1_SOLLC (Pectinesterase 1 OS=Solanum lycopersicum GN=PME1.9 PE=1 SV=5)

HSP 1 Score: 558.5 bits (1438), Expect = 1.0e-157
Identity = 300/537 (55.87%), Postives = 370/537 (68.90%), Query Frame = 1

Query: 160 PKTFFTILFFSLLLGSAIFITNKLINSNPSENDHHPFLHNICYKAYNQSSCIQMLASEFP 219
           P   F IL F + L  A+F    L+     E  H     N+C  A +   C+  ++    
Sbjct: 17  PIISFKILSFVITLFVALF----LVAPYQVEIKHS----NLCKTAQDSQLCLSYVSDLIS 76

Query: 220 LLQETKTTNAANFLQSFLRKSTSKIVNTIESAKDLRRRINNPRGEAALADCVELMEISMD 279
               T  ++  + L  FL     ++ N I   + ++ +IN+ R   AL DC+EL++ S+D
Sbjct: 77  NEIVTTESDGHSILMKFLVNYVHQMNNAIPVVRKMKNQINDIRQHGALTDCLELLDQSVD 136

Query: 280 RITDSILALERRRCGTIENAHTWLSSVLTNHVACSDEIENSISKAAAMDLGVEELIVIGR 339
             +DSI A+++R      NA +WLS VLTNHV C DE++ S +KA      +EELI   +
Sbjct: 137 FASDSIAAIDKRSRSEHANAQSWLSGVLTNHVTCLDELD-SFTKAMINGTNLEELISRAK 196

Query: 340 NSLGMLVSIWGSGKKEGMGY----PSWLKKGDRRLLEVVGRGMKADIVVAKDGSGNFKTV 399
            +L ML S+    +   M      PSW+   DR+L+E  G+ + A+ VVA+DG+G+++T+
Sbjct: 197 VALAMLASLTTQDEDVFMTVLGKMPSWVSSMDRKLMESSGKDIIANAVVAQDGTGDYQTL 256

Query: 400 KEAVEAVPDKSKSRIVIYVKKGIYKENVEVGKNKKNVMLVGDGMDSTIITASLNVVDGST 459
            EAV A PDKSK+R VIYVK+G YKENVEV  NK N+M+VGDGM +T IT SLNVVDGST
Sbjct: 257 AEAVAAAPDKSKTRYVIYVKRGTYKENVEVASNKMNLMIVGDGMYATTITGSLNVVDGST 316

Query: 460 TFKSATVAAVGDGFIAQDIWFRNTAEPEKHQAVALRVGADQSVINRCRIDAYQDTLYAHT 519
           TF+SAT+AAVG GFI QDI  +NTA P K QAVALRVGAD SVINRCRIDAYQDTLYAH+
Sbjct: 317 TFRSATLAAVGQGFILQDICIQNTAGPAKDQAVALRVGADMSVINRCRIDAYQDTLYAHS 376

Query: 520 NRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQANMVTAQGRTDPNQNTGTSIQ 579
            RQFYRDS +TGTVDFIFGNAAVV Q C++  R+P   Q NMVTAQGRTDPNQ TGTSIQ
Sbjct: 377 QRQFYRDSYVTGTVDFIFGNAAVVFQKCQLVARKPGKYQQNMVTAQGRTDPNQATGTSIQ 436

Query: 580 RCEIVASPDLEPVKKSIKTYLGRPWKEYSRTVVMQSTLGDLIDPAGWAEWNGEFALKTLY 639
            C I+AS DLEPV K   TYLGRPWKEYSRTVVM+S LG LI+PAGWAEW+G+FALKTLY
Sbjct: 437 FCNIIASSDLEPVLKEFPTYLGRPWKEYSRTVVMESYLGGLINPAGWAEWDGDFALKTLY 496

Query: 640 YGEYSNNGPGSNVSKRVKWPGYHVITSPAVAQKFTVDSLIQGAEWLGSSGVTFIPGL 693
           YGE+ NNGPG+  SKRVKWPGYHVIT PA A  FTV  LIQG  WL S+GV ++ GL
Sbjct: 497 YGEFMNNGPGAGTSKRVKWPGYHVITDPAKAMPFTVAKLIQGGSWLRSTGVAYVDGL 544

BLAST of CmaCh07G002990.1 vs. Swiss-Prot
Match: PME18_ARATH (Pectinesterase/pectinesterase inhibitor 18 OS=Arabidopsis thaliana GN=PME18 PE=1 SV=3)

HSP 1 Score: 555.1 bits (1429), Expect = 1.1e-156
Identity = 300/553 (54.25%), Postives = 383/553 (69.26%), Query Frame = 1

Query: 146 THEPLLPQPPETTRPKTFFTILFFSLLLGSAIFITNKLINSNPSENDHHPFLHN-ICYKA 205
           +++PLL +P ++ + K    +L F  +LGS  F T +LI+ N + ND      + IC+ A
Sbjct: 4   SNQPLLSKP-KSLKHKNLCLVLSFVAILGSVAFFTAQLISVNTNNNDDSLLTTSQICHGA 63

Query: 206 YNQSSCIQMLASEFPLLQETKTTNAANFLQSFLRKSTSKIVNTIESAKDLRRRINNPRGE 265
           ++Q SC Q L SEF  L  +K  N  + L  FL+ S  ++ +T+    + R R N  R +
Sbjct: 64  HDQDSC-QALLSEFTTLSLSKL-NRLDLLHVFLKNSVWRLESTMTMVSEARIRSNGVRDK 123

Query: 266 AALADCVELMEISMDRITDSILALE--RRRCGTIENAHTWLSSVLTNHVACSDEIEN-SI 325
           A  ADC E+M++S DR+  S+  L        +  N HTWLSSVLTN++ C + I + S+
Sbjct: 124 AGFADCEEMMDVSKDRMMSSMEELRGGNYNLESYSNVHTWLSSVLTNYMTCLESISDVSV 183

Query: 326 SKAAAMDLGVEELIVIGRNSLGMLVSIWGSGKKEGM----GYPSWLKKGDRRLLEVVGRG 385
           +    +   +E+L+   R +L + VS+  +     M     +PSWL   DR+LLE   + 
Sbjct: 184 NSKQIVKPQLEDLVSRARVALAIFVSVLPARDDLKMIISNRFPSWLTALDRKLLESSPKT 243

Query: 386 MK--ADIVVAKDGSGNFKTVKEAVEAVPDKSKSRIVIYVKKGIYKENVEVGKNKKNVMLV 445
           +K  A++VVAKDG+G FKTV EAV A P+ S +R VIYVKKG+YKE +++GK KKN+MLV
Sbjct: 244 LKVTANVVVAKDGTGKFKTVNEAVAAAPENSNTRYVIYVKKGVYKETIDIGKKKKNLMLV 303

Query: 446 GDGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAEPEKHQAVALRVGAD 505
           GDG D+TIIT SLNV+DGSTTF+SATVAA GDGF+AQDIWF+NTA P KHQAVALRV AD
Sbjct: 304 GDGKDATIITGSLNVIDGSTTFRSATVAANGDGFMAQDIWFQNTAGPAKHQAVALRVSAD 363

Query: 506 QSVINRCRIDAYQDTLYAHTNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQA 565
           Q+VINRCRIDAYQDTLY HT RQFYRDS ITGTVDFIFGN+AVV QNC I  R P + Q 
Sbjct: 364 QTVINRCRIDAYQDTLYTHTLRQFYRDSYITGTVDFIFGNSAVVFQNCDIVARNPGAGQK 423

Query: 566 NMVTAQGRTDPNQNTGTSIQRCEIVASPDLEPVKKSIKTYLGRPWKEYSRTVVMQSTLGD 625
           NM+TAQGR D NQNT  SIQ+C+I AS DL PVK S+KT+LGRPWK YSRTV+MQS + +
Sbjct: 424 NMLTAQGREDQNQNTAISIQKCKITASSDLAPVKGSVKTFLGRPWKLYSRTVIMQSFIDN 483

Query: 626 LIDPAGWAEWNGEFALKTLYYGEYSNNGPGSNVSKRVKWPGYHVITSPAVAQKFTVDSLI 685
            IDPAGW  W+GEFAL TLYYGEY+N GPG++ SKRV W G+ VI     A++FTV  LI
Sbjct: 484 HIDPAGWFPWDGEFALSTLYYGEYANTGPGADTSKRVNWKGFKVIKDSKEAEQFTVAKLI 543

Query: 686 QGAEWLGSSGVTF 689
           QG  WL  +GVTF
Sbjct: 544 QGGLWLKPTGVTF 553

BLAST of CmaCh07G002990.1 vs. TrEMBL
Match: A0A0A0KHV5_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_6G514890 PE=4 SV=1)

HSP 1 Score: 881.7 bits (2277), Expect = 5.8e-253
Identity = 446/557 (80.07%), Postives = 494/557 (88.69%), Query Frame = 1

Query: 144 MATHEPLLP-QPPETTRPKTFFTILFFSLLLGSAIFITNKLINSNPSENDHHPFLHNICY 203
           MATH+PLL  QPP+T+R KT  T+L F+ +L SAIFITNK I  NPSEND  PFL NIC+
Sbjct: 1   MATHQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSENDL-PFLQNICH 60

Query: 204 KAYNQSSCIQMLASEFPLLQETKTTNAANFLQSFLRKSTSKIVNTIESAKDLRRRINNPR 263
           KAY+ SSCI+M ASEFPL    KTTN  +FLQSFLRKS  K+++TIE AKD+R+RIN+PR
Sbjct: 61  KAYDPSSCIEMAASEFPL-SIIKTTNEVDFLQSFLRKSMPKVISTIERAKDIRQRINSPR 120

Query: 264 GEAALADCVELMEISMDRITDSILALERRRCGTIENAHTWLSSVLTNHVACSDEIENSIS 323
           GEAALADC+ELMEIS  RI DS+LAL+ R  G+IEN+HTWLSSVLTNHV C DE+E+S+S
Sbjct: 121 GEAALADCIELMEISNGRIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTCWDEVESSLS 180

Query: 324 KAAAMDLGVEELIVIGRNSLGMLVSIWGSG-------KKEGMGYPSWLKKGDRRLLEVVG 383
           +AA MDLG+EELI+ GRNSLGMLVSIWG         +K+G GYPSWLKKGDRRLL V+G
Sbjct: 181 RAAPMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLG 240

Query: 384 RGMKADIVVAKDGSGNFKTVKEAVEAVPDKSKSRIVIYVKKGIYKENVEVGKNKKNVMLV 443
           R M+ +IVVAKDGSGNFKTVKEAVE+VPDKSK+RIVIYVK+G Y+ENVEVGK KKNVM+V
Sbjct: 241 REMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIV 300

Query: 444 GDGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAEPEKHQAVALRVGAD 503
           GDGMDSTIIT SLNVVDGSTTFKSATVAAVGDGFIAQDIWF+NTA PEKHQAVALRVGAD
Sbjct: 301 GDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGAD 360

Query: 504 QSVINRCRIDAYQDTLYAHTNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQA 563
           QSVINRCRIDAYQDTLY H+NRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPM+NQA
Sbjct: 361 QSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQA 420

Query: 564 NMVTAQGRTDPNQNTGTSIQRCEIVASPDLEPVKKSIKTYLGRPWKEYSRTVVMQSTLGD 623
           NMVTAQGR DPNQNTGTSIQ+C+IVAS DLEPVKKSIKTYLGRPWKEYSRTV+MQS +GD
Sbjct: 421 NMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGD 480

Query: 624 LIDPAGWAEWNGEFALKTLYYGEYSNNGPGSNVSKRVKWPGYHVITSPAVAQKFTVDSLI 683
           LI PAGWAEWNG+FALKTLYYGEYSN GPGS+VSKRVKW GYH+ITSP+ AQKFTVDSLI
Sbjct: 481 LIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVDSLI 540

Query: 684 QGAEWLGSSGVTFIPGL 693
           QG EWLG SG TFIPGL
Sbjct: 541 QGGEWLGPSGATFIPGL 555

BLAST of CmaCh07G002990.1 vs. TrEMBL
Match: W9S4J9_9ROSA (Pectinesterase OS=Morus notabilis GN=L484_012896 PE=4 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 3.2e-187
Identity = 343/555 (61.80%), Postives = 415/555 (74.77%), Query Frame = 1

Query: 144 MATHEPLLPQPPETTRPKTFFTILFFSLLLGSAIFITNKLINSNPSENDHHPFLHNICYK 203
           MAT++PLL     +     +  ++  + L+ +A F  + +  ++ S   + P LH IC +
Sbjct: 1   MATNQPLLETSKSSHSKIPYLLLISIAALVSTAFFAIHSIKTTSLSTPPNLP-LHKICDQ 60

Query: 204 AYNQSSCIQMLASEFPLLQETKTTNAANFLQSFLRKSTSKIVNTIESAKDLRRRINNPRG 263
           + ++ SC+ M++      Q +   +  + L++FL K+T +I   ++ A D+R RINNPR 
Sbjct: 61  SVDKESCLAMVSEA--ARQYSTVADHHDLLKAFLEKTTPRIQQALKMANDVRLRINNPRD 120

Query: 264 EAALADCVELMEISMDRITDSI--LALERRRCGTIENAHTWLSSVLTNHVACSDEIENSI 323
            A L DC ELM+IS DR+ DSI  L L+++   + E+ H WLSSVLTNHV C D +E+  
Sbjct: 121 RATLLDCAELMDISKDRVVDSISTLFLQKQTSHSHEDVHAWLSSVLTNHVTCLDALEDGS 180

Query: 324 SKAAAMDLGVEELIVIGRNSLGMLVSIWGSGKKE----GMGYPSWLKKGDRRLLEVVGRG 383
                MD  + ELI+  R SL +LVS++ S  +         P+W+  GDRRLL+   + 
Sbjct: 181 YAKNLMDSHLNELILRARTSLAILVSVFPSNSEAIETLTDDLPTWVTAGDRRLLQASVKA 240

Query: 384 MKADIVVAKDGSGNFKTVKEAVEAVPDKSKSRIVIYVKKGIYKENVEVGKNKKNVMLVGD 443
           +KADIVVA+DGSG +KTVKEAV AVPD SK+R  IYVKKG YKENVE+GK KKNVMLVGD
Sbjct: 241 IKADIVVAEDGSGKYKTVKEAVAAVPDNSKTRFTIYVKKGTYKENVEIGKKKKNVMLVGD 300

Query: 444 GMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAEPEKHQAVALRVGADQS 503
           GMDSTIIT SLNV+DGSTTF SATVAAVGDGFIAQDIWF+NTA P+KHQAVALRVGADQS
Sbjct: 301 GMDSTIITGSLNVIDGSTTFNSATVAAVGDGFIAQDIWFQNTAGPQKHQAVALRVGADQS 360

Query: 504 VINRCRIDAYQDTLYAHTNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQANM 563
           VINRCRIDAYQDTLYAH+NRQFYRDST+TGTVDFIFGNAAVV QNCK+  R+ MS Q NM
Sbjct: 361 VINRCRIDAYQDTLYAHSNRQFYRDSTVTGTVDFIFGNAAVVFQNCKLVARKGMSGQKNM 420

Query: 564 VTAQGRTDPNQNTGTSIQRCEIVASPDLEPVKKSIKTYLGRPWKEYSRTVVMQSTLGDLI 623
           VTAQGRTDPNQNTGTSIQ+CEI AS DLEPVK S K+YLGRPWKEYSRTVVMQST+GDLI
Sbjct: 421 VTAQGRTDPNQNTGTSIQKCEISASSDLEPVKASYKSYLGRPWKEYSRTVVMQSTIGDLI 480

Query: 624 DPAGWAEWNGEFALKTLYYGEYSNNGPGSNVSKRVKWPGYHVITSPAVAQKFTVDSLIQG 683
           DPAGWAEW+ +FALKTLYYGEY+N GPG+  SKRV W GYHVITSP+ AQKFTV  LIQG
Sbjct: 481 DPAGWAEWDKDFALKTLYYGEYANQGPGAGTSKRVNWAGYHVITSPSEAQKFTVAELIQG 540

Query: 684 AEWLGSSGVTFIPGL 693
             WL +SGV +  GL
Sbjct: 541 GYWLKNSGVAYTEGL 552

BLAST of CmaCh07G002990.1 vs. TrEMBL
Match: A0A067G7D1_CITSI (Pectinesterase OS=Citrus sinensis GN=CISIN_1g008722mg PE=4 SV=1)

HSP 1 Score: 659.4 bits (1700), Expect = 4.7e-186
Identity = 346/547 (63.25%), Postives = 408/547 (74.59%), Query Frame = 1

Query: 155 PETTRPKTFFTILFFSLLLGSAIFITNKLINSNPSENDHHPFLHNICYKAYNQSSCIQML 214
           P+ ++ K    IL    ++ SA F+    I +  S N  H  LH  C KA +QSSCI M+
Sbjct: 15  PKASKRKILLLILSVVSVMCSATFVAIHCIKAINSNN--HWSLHQFCAKAQDQSSCIAMV 74

Query: 215 ASEFPLLQETKTTNAANFLQSFLRKSTSKIVNTIESAKDLRRRINNPRGEAALADCVELM 274
            SE   +  T   N  + L  FL KSTS I  TIE A  ++ RIN+PRG+AAL DCVEL+
Sbjct: 75  -SETTSINATTKMNEVDLLLIFLSKSTSNIQETIEVANHVKHRINSPRGQAALTDCVELL 134

Query: 275 EISMDRITDSILALERRRCGTIENAHTWLSSVLTNHVACSDEIENSISKAAAMDLGVEEL 334
           E+S+DRITDS+ AL++RR  + E+AH WLS VLTNHV C D ++ S +K   M+  + EL
Sbjct: 135 ELSVDRITDSMAALKKRRTSSNEDAHAWLSGVLTNHVTCLDGLDGSATKL--MEPRLVEL 194

Query: 335 IVIGRNSLGMLVSIWGSGK--------KEGMGYPSWLKKGDRRLLEVV-GRGMKADIVVA 394
           +    NSL +LV+I             ++ + +PSW+   DR LL        KA++VVA
Sbjct: 195 MARAANSLAILVAISPYNTNVIQQYYLRKEIQFPSWVTSRDRLLLRPSQAEAAKANVVVA 254

Query: 395 KDGSGNFKTVKEAVEAVPDKSKSRIVIYVKKGIYKENVEVGKNKKNVMLVGDGMDSTIIT 454
           KDGSGN+KTVKEAV + PD SK+R VIYVKKG YKENVEVGK KKN+M+VGDGMD TIIT
Sbjct: 255 KDGSGNYKTVKEAVASAPDNSKTRYVIYVKKGTYKENVEVGKKKKNLMIVGDGMDLTIIT 314

Query: 455 ASLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAEPEKHQAVALRVGADQSVINRCRID 514
            SLNVVDGSTTFKSAT+A  GDGFIAQD+W +NTA PEKHQAVALRV ADQSVINRCRID
Sbjct: 315 GSLNVVDGSTTFKSATIAVGGDGFIAQDLWIQNTAGPEKHQAVALRVSADQSVINRCRID 374

Query: 515 AYQDTLYAHTNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQANMVTAQGRTD 574
           AYQDTLYAHTNRQFYRD  ITGTVDFIFGNAAVVLQNCKI  R+PMS Q+NMVTAQGRTD
Sbjct: 375 AYQDTLYAHTNRQFYRDCYITGTVDFIFGNAAVVLQNCKIAARKPMSKQSNMVTAQGRTD 434

Query: 575 PNQNTGTSIQRCEIVASPDLEPVKKSIKTYLGRPWKEYSRTVVMQSTLGDLIDPAGWAEW 634
           PNQNTGTSIQ+C+++AS DLEPVK SI++YLGRPWKEYSRTVVMQS +GD IDPAGW+EW
Sbjct: 435 PNQNTGTSIQKCDVIASSDLEPVKGSIRSYLGRPWKEYSRTVVMQSHIGDHIDPAGWSEW 494

Query: 635 NGEFALKTLYYGEYSNNGPGSNVSKRVKWPGYHVITSPAVAQKFTVDSLIQGAEWLGSSG 693
           +G+FALKTLYYGEY N GPG+  SKRVKWPGYHVIT P  A+KFTV  LI G  WL S+G
Sbjct: 495 SGDFALKTLYYGEYLNRGPGAGTSKRVKWPGYHVITDPEEAKKFTVAELIGGGSWLKSTG 554

BLAST of CmaCh07G002990.1 vs. TrEMBL
Match: V4UJ35_9ROSI (Pectinesterase OS=Citrus clementina GN=CICLE_v10014787mg PE=4 SV=1)

HSP 1 Score: 656.0 bits (1691), Expect = 5.1e-185
Identity = 345/547 (63.07%), Postives = 407/547 (74.41%), Query Frame = 1

Query: 155 PETTRPKTFFTILFFSLLLGSAIFITNKLINSNPSENDHHPFLHNICYKAYNQSSCIQML 214
           P+ ++ K    IL    ++ SA F+    I +  S N  H  LH IC  A +QSSC+ M+
Sbjct: 15  PKASKRKILLLILSVVSVMCSATFVAIHCIKAINSNN--HWSLHQICANARDQSSCMAMV 74

Query: 215 ASEFPLLQETKTTNAANFLQSFLRKSTSKIVNTIESAKDLRRRINNPRGEAALADCVELM 274
            SE   +  T   N  + L  FL KSTS I  TIE A  ++ RIN+PRG+AAL DCVEL+
Sbjct: 75  -SETTSINATTKMNEVDLLLIFLNKSTSYIQETIEVANHVKHRINSPRGQAALTDCVELL 134

Query: 275 EISMDRITDSILALERRRCGTIENAHTWLSSVLTNHVACSDEIENSISKAAAMDLGVEEL 334
           E+S+DRITDS+ AL++RR  + E+AH WLS VLTNHV C D ++ S +K   M+  + EL
Sbjct: 135 ELSVDRITDSMAALKKRRTSSNEDAHAWLSGVLTNHVTCLDGLDGSATKL--MEPRLVEL 194

Query: 335 IVIGRNSLGMLVSIWGSGK--------KEGMGYPSWLKKGDRRLLEVV-GRGMKADIVVA 394
           +    NSL +LV+I             ++ + +PSW+   DR LL        KA++VVA
Sbjct: 195 MARAANSLAILVAISPYNTNVIQQYYLRKEIQFPSWVTSRDRLLLRPSQAEAAKANVVVA 254

Query: 395 KDGSGNFKTVKEAVEAVPDKSKSRIVIYVKKGIYKENVEVGKNKKNVMLVGDGMDSTIIT 454
           KDGSGN+KTVKEAV + PD SK+R VIYVKKG YKENVEVGK KKN+M+VGDGMD TIIT
Sbjct: 255 KDGSGNYKTVKEAVASAPDNSKTRYVIYVKKGTYKENVEVGKKKKNLMIVGDGMDLTIIT 314

Query: 455 ASLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAEPEKHQAVALRVGADQSVINRCRID 514
            SLNVVDGSTTFKSAT+A  GDGFIAQD+W +NTA PEKHQAVALRV ADQSVINRCRID
Sbjct: 315 GSLNVVDGSTTFKSATIAVGGDGFIAQDLWIQNTAGPEKHQAVALRVSADQSVINRCRID 374

Query: 515 AYQDTLYAHTNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQANMVTAQGRTD 574
           AYQDTLYAHTNRQFYRD  ITGTVDFIFGNAAVVLQNCKI  R+PMS Q+NMVTAQGRTD
Sbjct: 375 AYQDTLYAHTNRQFYRDCYITGTVDFIFGNAAVVLQNCKIAARKPMSKQSNMVTAQGRTD 434

Query: 575 PNQNTGTSIQRCEIVASPDLEPVKKSIKTYLGRPWKEYSRTVVMQSTLGDLIDPAGWAEW 634
           PNQNTGTSIQ+CE++AS DLEPVK SI++YLGRPWKEYSRTVVMQS +G  IDPAGW+EW
Sbjct: 435 PNQNTGTSIQKCEVIASSDLEPVKGSIRSYLGRPWKEYSRTVVMQSHIGGHIDPAGWSEW 494

Query: 635 NGEFALKTLYYGEYSNNGPGSNVSKRVKWPGYHVITSPAVAQKFTVDSLIQGAEWLGSSG 693
           +G+FALKTLYYGEY N GPG+  SKRVKWPGYHVIT P  A+KFTV  LI G  WL S+G
Sbjct: 495 SGDFALKTLYYGEYLNRGPGAGTSKRVKWPGYHVITDPGEAKKFTVAELIGGGSWLKSTG 554

BLAST of CmaCh07G002990.1 vs. TrEMBL
Match: B9HZL3_POPTR (Pectinesterase OS=Populus trichocarpa GN=POPTR_0011s00480g PE=4 SV=1)

HSP 1 Score: 638.6 bits (1646), Expect = 8.5e-180
Identity = 345/556 (62.05%), Postives = 400/556 (71.94%), Query Frame = 1

Query: 144 MATHEPLLPQPPETTRPKTFFTILFFSLLLGSAIFITNKLIN-SNPSENDHHPFLHNICY 203
           MAT    L   P+T+R KT   +L    +L S   +T +    ++PS       L N+C 
Sbjct: 1   MATMNTPLLATPKTSRRKTICLVLSMVAILSSTTLVTMRYYTKTDPSSPPG--LLQNLCD 60

Query: 204 KAYNQSSCIQMLASEFPLLQETKTT-NAANFLQSFLRKSTSKIVNTIESAKDLRRRINNP 263
            AY+Q SC+ M++    +   T T  +    LQ  L KST  I NTIE A+ +  RIN+ 
Sbjct: 61  HAYDQESCLAMVSQ---IASNTSTKMSQVGLLQLLLGKSTPHIQNTIEKAEVIHSRINDA 120

Query: 264 RGEAALADCVELMEISMDRITDSILALERRRCGTIENAHTWLSSVLTNHVACSDEIENSI 323
           R +AAL DCVELMEIS  RI D+I+ALER    +  NA TWLSSVLTNH  C D +    
Sbjct: 121 REQAALGDCVELMEISKYRIKDTIVALERVTSKSHANALTWLSSVLTNHDTCLDGLNGPA 180

Query: 324 SKAAAMDLGVEELIVIGRNSLGMLVSIWGSGKKEGM-----GYPSWLKKGDRRLLEVVGR 383
                 DL   +LI+  R SL +L +I  S +   +      +PSWL   DR+LL  + +
Sbjct: 181 RSTMEPDLN--DLILRARTSLAILAAISPSKENNDIFSLKEDFPSWLPSMDRKLLVALPK 240

Query: 384 GMKADIVVAKDGSGNFKTVKEAVEAVPDKSKSRIVIYVKKGIYKENVEVGKNKKNVMLVG 443
            + AD+ VAKDGSG +KTVKEAV + PD  K+R VIYVKKG YKENVEVGK KKNVMLVG
Sbjct: 241 DINADVTVAKDGSGKYKTVKEAVASAPDNGKTRYVIYVKKGTYKENVEVGKKKKNVMLVG 300

Query: 444 DGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAEPEKHQAVALRVGADQ 503
           DGMDSTIIT SLNVVDGSTTF SATVAAVGDGFIAQDIWF+NTA PEKHQAVALRVGADQ
Sbjct: 301 DGMDSTIITGSLNVVDGSTTFNSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQ 360

Query: 504 SVINRCRIDAYQDTLYAHTNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQAN 563
           SVINRCRIDAYQDTLY H+ RQFYRDS ITGTVDFIFGNAAVVLQNCK+ PR+PMS Q N
Sbjct: 361 SVINRCRIDAYQDTLYTHSLRQFYRDSYITGTVDFIFGNAAVVLQNCKLVPRKPMSGQKN 420

Query: 564 MVTAQGRTDPNQNTGTSIQRCEIVASPDLEPVKKSIKTYLGRPWKEYSRTVVMQSTLGDL 623
           MVTAQGRTDPNQNTGTSIQ+C+I+AS DL PVK S K++LGRPWKEYSRTVVMQS +GDL
Sbjct: 421 MVTAQGRTDPNQNTGTSIQKCDIIASSDLTPVKSSFKSFLGRPWKEYSRTVVMQSNIGDL 480

Query: 624 IDPAGWAEWNGEFALKTLYYGEYSNNGPGSNVSKRVKWPGYHVITSPAVAQKFTVDSLIQ 683
           IDPAGW+ W+GEFALKTLYYGEY N G G+  SKRV W GYHVITS   A+KFTV  LIQ
Sbjct: 481 IDPAGWSAWDGEFALKTLYYGEYLNQGAGAGTSKRVNWAGYHVITSANEAKKFTVAELIQ 540

Query: 684 GAEWLGSSGVTFIPGL 693
           G  WL S+GV++  GL
Sbjct: 541 GGVWLKSTGVSYTEGL 549

BLAST of CmaCh07G002990.1 vs. TAIR10
Match: AT1G11580.1 (AT1G11580.1 methylesterase PCR A)

HSP 1 Score: 555.1 bits (1429), Expect = 6.3e-158
Identity = 300/553 (54.25%), Postives = 383/553 (69.26%), Query Frame = 1

Query: 146 THEPLLPQPPETTRPKTFFTILFFSLLLGSAIFITNKLINSNPSENDHHPFLHN-ICYKA 205
           +++PLL +P ++ + K    +L F  +LGS  F T +LI+ N + ND      + IC+ A
Sbjct: 4   SNQPLLSKP-KSLKHKNLCLVLSFVAILGSVAFFTAQLISVNTNNNDDSLLTTSQICHGA 63

Query: 206 YNQSSCIQMLASEFPLLQETKTTNAANFLQSFLRKSTSKIVNTIESAKDLRRRINNPRGE 265
           ++Q SC Q L SEF  L  +K  N  + L  FL+ S  ++ +T+    + R R N  R +
Sbjct: 64  HDQDSC-QALLSEFTTLSLSKL-NRLDLLHVFLKNSVWRLESTMTMVSEARIRSNGVRDK 123

Query: 266 AALADCVELMEISMDRITDSILALE--RRRCGTIENAHTWLSSVLTNHVACSDEIEN-SI 325
           A  ADC E+M++S DR+  S+  L        +  N HTWLSSVLTN++ C + I + S+
Sbjct: 124 AGFADCEEMMDVSKDRMMSSMEELRGGNYNLESYSNVHTWLSSVLTNYMTCLESISDVSV 183

Query: 326 SKAAAMDLGVEELIVIGRNSLGMLVSIWGSGKKEGM----GYPSWLKKGDRRLLEVVGRG 385
           +    +   +E+L+   R +L + VS+  +     M     +PSWL   DR+LLE   + 
Sbjct: 184 NSKQIVKPQLEDLVSRARVALAIFVSVLPARDDLKMIISNRFPSWLTALDRKLLESSPKT 243

Query: 386 MK--ADIVVAKDGSGNFKTVKEAVEAVPDKSKSRIVIYVKKGIYKENVEVGKNKKNVMLV 445
           +K  A++VVAKDG+G FKTV EAV A P+ S +R VIYVKKG+YKE +++GK KKN+MLV
Sbjct: 244 LKVTANVVVAKDGTGKFKTVNEAVAAAPENSNTRYVIYVKKGVYKETIDIGKKKKNLMLV 303

Query: 446 GDGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAEPEKHQAVALRVGAD 505
           GDG D+TIIT SLNV+DGSTTF+SATVAA GDGF+AQDIWF+NTA P KHQAVALRV AD
Sbjct: 304 GDGKDATIITGSLNVIDGSTTFRSATVAANGDGFMAQDIWFQNTAGPAKHQAVALRVSAD 363

Query: 506 QSVINRCRIDAYQDTLYAHTNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQA 565
           Q+VINRCRIDAYQDTLY HT RQFYRDS ITGTVDFIFGN+AVV QNC I  R P + Q 
Sbjct: 364 QTVINRCRIDAYQDTLYTHTLRQFYRDSYITGTVDFIFGNSAVVFQNCDIVARNPGAGQK 423

Query: 566 NMVTAQGRTDPNQNTGTSIQRCEIVASPDLEPVKKSIKTYLGRPWKEYSRTVVMQSTLGD 625
           NM+TAQGR D NQNT  SIQ+C+I AS DL PVK S+KT+LGRPWK YSRTV+MQS + +
Sbjct: 424 NMLTAQGREDQNQNTAISIQKCKITASSDLAPVKGSVKTFLGRPWKLYSRTVIMQSFIDN 483

Query: 626 LIDPAGWAEWNGEFALKTLYYGEYSNNGPGSNVSKRVKWPGYHVITSPAVAQKFTVDSLI 685
            IDPAGW  W+GEFAL TLYYGEY+N GPG++ SKRV W G+ VI     A++FTV  LI
Sbjct: 484 HIDPAGWFPWDGEFALSTLYYGEYANTGPGADTSKRVNWKGFKVIKDSKEAEQFTVAKLI 543

Query: 686 QGAEWLGSSGVTF 689
           QG  WL  +GVTF
Sbjct: 544 QGGLWLKPTGVTF 553

BLAST of CmaCh07G002990.1 vs. TAIR10
Match: AT3G14310.1 (AT3G14310.1 pectin methylesterase 3)

HSP 1 Score: 429.5 bits (1103), Expect = 4.0e-120
Identity = 216/347 (62.25%), Postives = 253/347 (72.91%), Query Frame = 1

Query: 346 VSIWGSGKKEGMGYPSWLKKGDRRLLEVVGRGMKADIVVAKDGSGNFKTVKEAVEAVPDK 405
           V I G+G+ +  G+P+WL  GDRRLL+  G G+KAD  VA DGSG FKTV  AV A P+ 
Sbjct: 248 VDIAGAGELDSEGWPTWLSAGDRRLLQ--GSGVKADATVAADGSGTFKTVAAAVAAAPEN 307

Query: 406 SKSRIVIYVKKGIYKENVEVGKNKKNVMLVGDGMDSTIITASLNVVDGSTTFKSATVAAV 465
           S  R VI++K G+Y+ENVEV K KKN+M +GDG   TIIT S NVVDGSTTF SATVAAV
Sbjct: 308 SNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAV 367

Query: 466 GDGFIAQDIWFRNTAEPEKHQAVALRVGADQSVINRCRIDAYQDTLYAHTNRQFYRDSTI 525
           G+ F+A+DI F+NTA P KHQAVALRVG+D S    C + AYQDTLY H+NRQF+    I
Sbjct: 368 GERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLI 427

Query: 526 TGTVDFIFGNAAVVLQNCKIEPRRPMSNQANMVTAQGRTDPNQNTGTSIQRCEIVASPDL 585
            GTVDFIFGNAAVVLQ+C I  RRP S Q NMVTAQGRTDPNQNTG  IQ+C I A+ DL
Sbjct: 428 AGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDL 487

Query: 586 EPVKKSIKTYLGRPWKEYSRTVVMQSTLGDLIDPAGWAEWNGEFALKTLYYGEYSNNGPG 645
           + VK S  TYLGRPWKEYS+TV+MQS + D+I P GW+EW G FAL TL Y EYSN G G
Sbjct: 488 QSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAG 547

Query: 646 SNVSKRVKWPGYHVITSPAVAQKFTVDSLIQGAEWLGSSGVTFIPGL 693
           +  + RVKW G+ VIT+ A AQK+T    I G  WL S+G  F  GL
Sbjct: 548 AGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of CmaCh07G002990.1 vs. TAIR10
Match: AT5G53370.1 (AT5G53370.1 pectin methylesterase PCR fragment F)

HSP 1 Score: 424.5 bits (1090), Expect = 1.3e-118
Identity = 236/513 (46.00%), Postives = 310/513 (60.43%), Query Frame = 1

Query: 201 CYKAYNQSSCIQMLASEFPLLQETKTTNAANFLQSFLRKSTSKIVNTIESAKDLRRRINN 260
           C K+   + CI  L  +FP    + T +    +      +  K    + ++  +      
Sbjct: 79  CSKSLYPNLCIDTLL-DFP---GSLTADENELIHISFNATLQKFSKALYTSSTITYTQMP 138

Query: 261 PRGEAALADCVELMEISMDRIT---DSILALERRRCGTIENAHTWLSSVLTNHVACSD-- 320
           PR  +A   C+EL++ S+D +T    S++ +      +  +  TWLSS +TNH  C+D  
Sbjct: 139 PRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHS--DVMTWLSSAMTNHDTCTDGF 198

Query: 321 -EIENSISKAAAMDLG-VEELIVIGRNSL----GMLVSIWGS---------GKKEGMGYP 380
            EIE    +     +G V++L  +  N L    G +  + G          G +E    P
Sbjct: 199 DEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEETEELP 258

Query: 381 SWLKKGDRRLLEVVGRGMKADIVVAKDGSGNFKTVKEAVEAVPDKSKSRIVIYVKKGIY- 440
           +WLK+ DR LL      ++ADI V+KDGSG FKT+ EA++  P+ S  R VIYVK G Y 
Sbjct: 259 NWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIYVKAGRYE 318

Query: 441 KENVEVGKNKKNVMLVGDGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNT 500
           +EN++VG+ K N+M +GDG   T+IT   ++ D  TTF +AT AA G GFI +D+ F N 
Sbjct: 319 EENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGFIVRDMTFENY 378

Query: 501 AEPEKHQAVALRVGADQSVINRCRIDAYQDTLYAHTNRQFYRDSTITGTVDFIFGNAAVV 560
           A P KHQAVALRVG D +V+ RC I  YQD LY H+NRQF+R+  I GTVDFIFGNAAV+
Sbjct: 379 AGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFIFGNAAVI 438

Query: 561 LQNCKIEPRRPMSNQANMVTAQGRTDPNQNTGTSIQRCEIVASPDLEPVKKSIKTYLGRP 620
           LQ+C I  R+PM+ Q   +TAQ R DPNQNTG SI  C+++A+PDLE  K S  TYLGRP
Sbjct: 439 LQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGSYPTYLGRP 498

Query: 621 WKEYSRTVVMQSTLGDLIDPAGWAEWNGEFALKTLYYGEYSNNGPGSNVSKRVKWPGYHV 680
           WK YSR V M S +GD IDP GW EWNG FAL +LYYGEY N G GS + +RVKWPGYHV
Sbjct: 499 WKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIGQRVKWPGYHV 558

Query: 681 ITSPAVAQKFTVDSLIQGAEWLGSSGVTFIPGL 693
           ITS   A KFTV   I G+ WL S+GV+F  GL
Sbjct: 559 ITSTVEASKFTVAQFISGSSWLPSTGVSFFSGL 585

BLAST of CmaCh07G002990.1 vs. TAIR10
Match: AT1G53830.1 (AT1G53830.1 pectin methylesterase 2)

HSP 1 Score: 421.4 bits (1082), Expect = 1.1e-117
Identity = 249/562 (44.31%), Postives = 321/562 (57.12%), Query Frame = 1

Query: 171 LLLGSAIFITNKLINSNPSE------NDHHPFLHNICYKAYNQSSCIQMLASEFPLLQET 230
           LLL S + I     N N ++      +  H  L ++C        C   +A+        
Sbjct: 30  LLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPELCFSAVAAT----GGK 89

Query: 231 KTTNAANFLQSFLRKSTSKIVNTIESAKDL--RRRINNPRGEAALADCVELMEISMDRIT 290
           + T+    +++ L  +T  + +   + K L  +R+   PR   AL DC+E ++ ++D + 
Sbjct: 90  ELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDCLETIDETLDELH 149

Query: 291 DSILAL-----ERRRCGTIENAHTWLSSVLTNHVACSD--------------------EI 350
            ++  L     ++      ++  T +SS +TN   C D                     +
Sbjct: 150 VAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRKVRKALLKGQVHV 209

Query: 351 ENSISKAAAMDLGVEELIVIG-----------RNSLGMLVSIWGSGKKEGMGYPSWLKKG 410
           E+  S A AM   + E  +              N+   L  +  +G  +  G+P WL  G
Sbjct: 210 EHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEV--TGDLDSDGWPKWLSVG 269

Query: 411 DRRLLEVVGRGMKADIVVAKDGSGNFKTVKEAVEAVPDKSKSRIVIYVKKGIYKENVEVG 470
           DRRLL+  G  +KAD  VA DGSG+F TV  AV A P+KS  R VI++K G+Y+ENVEV 
Sbjct: 270 DRRLLQ--GSTIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIHIKAGVYRENVEVT 329

Query: 471 KNKKNVMLVGDGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAEPEKHQ 530
           K K N+M +GDG   TIIT S NVVDGSTTF SATVAAVG+ F+A+DI F+NTA P KHQ
Sbjct: 330 KKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLARDITFQNTAGPSKHQ 389

Query: 531 AVALRVGADQSVINRCRIDAYQDTLYAHTNRQFYRDSTITGTVDFIFGNAAVVLQNCKIE 590
           AVALRVG+D S   +C + AYQDTLY H+NRQF+    ITGTVDFIFGNAA VLQ+C I 
Sbjct: 390 AVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFIFGNAAAVLQDCDIN 449

Query: 591 PRRPMSNQANMVTAQGRTDPNQNTGTSIQRCEIVASPDLEPVKKSIKTYLGRPWKEYSRT 650
            RRP S Q NMVTAQGR+DPNQNTG  IQ C I  + DL  VK +  TYLGRPWKEYSRT
Sbjct: 450 ARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTFPTYLGRPWKEYSRT 509

Query: 651 VVMQSTLGDLIDPAGWAEWNGEFALKTLYYGEYSNNGPGSNVSKRVKWPGYHVITSPAVA 689
           V+MQS + D+I P GW EW+G FAL TL Y EY N G G+  + RVKW GY VITS   A
Sbjct: 510 VIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRVKWKGYKVITSDTEA 569

BLAST of CmaCh07G002990.1 vs. TAIR10
Match: AT2G45220.1 (AT2G45220.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 414.1 bits (1063), Expect = 1.7e-115
Identity = 229/472 (48.52%), Postives = 304/472 (64.41%), Query Frame = 1

Query: 230 ANFLQSFLRKSTSKIVNTIESAKDLRRRINNPRGEAALADCVELMEISMDRITDSILALE 289
           + FL+  ++    + +     A  L  +  + R +AA  DC++L ++++ +I +++    
Sbjct: 60  SEFLKISMKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDP-- 119

Query: 290 RRRCGTIENAHTWLSSVLTNHVACSDEIENSISKAAAMDLGVEELIV--IGRNSLGMLVS 349
             +C  ++ A TWLS+ LTN   C         +A  ++LGV ++++  +  N   +L +
Sbjct: 120 NVKCSKLD-AQTWLSTALTNLDTC---------RAGFLELGVTDIVLPLMSNNVSNLLCN 179

Query: 350 IWGSGK-------KEGMGYPSWLKKGDRRLLEVVGRGMKADIVVAKDGSGNFKTVKEAVE 409
                K        E  G+PSW+K GDR+LL+      K + VVAKDGSGNFKT+KEA++
Sbjct: 180 TLAINKVPFNYTPPEKDGFPSWVKPGDRKLLQ--SSTPKDNAVVAKDGSGNFKTIKEAID 239

Query: 410 AVPDKSKSRIVIYVKKGIYKENVEVGKNKKNVMLVGDGMDSTIITASLNVVDGSTTFKSA 469
           A       R VIYVK+G+Y EN+E+   KKNVML GDG+  TIIT S +V  G+TTF SA
Sbjct: 240 AA--SGSGRFVIYVKQGVYSENLEI--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSA 299

Query: 470 TVAAVGDGFIAQDIWFRNTAEPEKHQAVALRVGADQSVINRCRIDAYQDTLYAHTNRQFY 529
           TVAAVGDGFIA+ I FRNTA     QAVALR G+D SV  +C  +AYQDTLY H+NRQFY
Sbjct: 300 TVAAVGDGFIARGITFRNTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFY 359

Query: 530 RDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQANMVTAQGRTDPNQNTGTSIQRCEIV 589
           RD  + GTVDFIFGNAA VLQNC I  RRP S + N +TAQGR+DPNQNTG  I    + 
Sbjct: 360 RDCDVYGTVDFIFGNAAAVLQNCNIFARRPRS-KTNTITAQGRSDPNQNTGIIIHNSRVT 419

Query: 590 ASPDLEPVKKSIKTYLGRPWKEYSRTVVMQSTLGDLIDPAGWAEWNGEFALKTLYYGEYS 649
           A+ DL PV  S KTYLGRPW++YSRTV M+++L  LIDP GW EW+G FALKTL+Y E+ 
Sbjct: 420 AASDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQ 479

Query: 650 NNGPGSNVSKRVKWPGYHVITSPAVAQKFTVDSLIQGAEWLGSSGVTFIPGL 693
           N GPG++ S RV WPG+ V+ S + A KFTV + + G  W+ SS V F  GL
Sbjct: 480 NTGPGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

BLAST of CmaCh07G002990.1 vs. NCBI nr
Match: gi|449433936|ref|XP_004134752.1| (PREDICTED: pectinesterase [Cucumis sativus])

HSP 1 Score: 881.7 bits (2277), Expect = 8.3e-253
Identity = 446/557 (80.07%), Postives = 494/557 (88.69%), Query Frame = 1

Query: 144 MATHEPLLP-QPPETTRPKTFFTILFFSLLLGSAIFITNKLINSNPSENDHHPFLHNICY 203
           MATH+PLL  QPP+T+R KT  T+L F+ +L SAIFITNK I  NPSEND  PFL NIC+
Sbjct: 1   MATHQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSENDL-PFLQNICH 60

Query: 204 KAYNQSSCIQMLASEFPLLQETKTTNAANFLQSFLRKSTSKIVNTIESAKDLRRRINNPR 263
           KAY+ SSCI+M ASEFPL    KTTN  +FLQSFLRKS  K+++TIE AKD+R+RIN+PR
Sbjct: 61  KAYDPSSCIEMAASEFPL-SIIKTTNEVDFLQSFLRKSMPKVISTIERAKDIRQRINSPR 120

Query: 264 GEAALADCVELMEISMDRITDSILALERRRCGTIENAHTWLSSVLTNHVACSDEIENSIS 323
           GEAALADC+ELMEIS  RI DS+LAL+ R  G+IEN+HTWLSSVLTNHV C DE+E+S+S
Sbjct: 121 GEAALADCIELMEISNGRIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTCWDEVESSLS 180

Query: 324 KAAAMDLGVEELIVIGRNSLGMLVSIWGSG-------KKEGMGYPSWLKKGDRRLLEVVG 383
           +AA MDLG+EELI+ GRNSLGMLVSIWG         +K+G GYPSWLKKGDRRLL V+G
Sbjct: 181 RAAPMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVLG 240

Query: 384 RGMKADIVVAKDGSGNFKTVKEAVEAVPDKSKSRIVIYVKKGIYKENVEVGKNKKNVMLV 443
           R M+ +IVVAKDGSGNFKTVKEAVE+VPDKSK+RIVIYVK+G Y+ENVEVGK KKNVM+V
Sbjct: 241 REMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMIV 300

Query: 444 GDGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAEPEKHQAVALRVGAD 503
           GDGMDSTIIT SLNVVDGSTTFKSATVAAVGDGFIAQDIWF+NTA PEKHQAVALRVGAD
Sbjct: 301 GDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGAD 360

Query: 504 QSVINRCRIDAYQDTLYAHTNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQA 563
           QSVINRCRIDAYQDTLY H+NRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPM+NQA
Sbjct: 361 QSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQA 420

Query: 564 NMVTAQGRTDPNQNTGTSIQRCEIVASPDLEPVKKSIKTYLGRPWKEYSRTVVMQSTLGD 623
           NMVTAQGR DPNQNTGTSIQ+C+IVAS DLEPVKKSIKTYLGRPWKEYSRTV+MQS +GD
Sbjct: 421 NMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIGD 480

Query: 624 LIDPAGWAEWNGEFALKTLYYGEYSNNGPGSNVSKRVKWPGYHVITSPAVAQKFTVDSLI 683
           LI PAGWAEWNG+FALKTLYYGEYSN GPGS+VSKRVKW GYH+ITSP+ AQKFTVDSLI
Sbjct: 481 LIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVDSLI 540

Query: 684 QGAEWLGSSGVTFIPGL 693
           QG EWLG SG TFIPGL
Sbjct: 541 QGGEWLGPSGATFIPGL 555

BLAST of CmaCh07G002990.1 vs. NCBI nr
Match: gi|659078898|ref|XP_008439965.1| (PREDICTED: pectinesterase [Cucumis melo])

HSP 1 Score: 870.5 bits (2248), Expect = 1.9e-249
Identity = 444/557 (79.71%), Postives = 489/557 (87.79%), Query Frame = 1

Query: 144 MATHEPLLP-QPPETTRPKTFFTILFFSLLLGSAIFITNKLINSNPSENDHHPFLHNICY 203
           MATH+PLL  QPP+T+  KT  TIL F+++L SAIF+TNK I  NP END  PFLHNIC+
Sbjct: 1   MATHQPLLHLQPPKTSCSKTISTILCFAVVLCSAIFVTNKFIKLNPYENDL-PFLHNICH 60

Query: 204 KAYNQSSCIQMLASEFPLLQETKTTNAANFLQSFLRKSTSKIVNTIESAKDLRRRINNPR 263
           KAYN SSCI+M ASEFPL    KTTN  +FLQSFL+KS  K++NTIE AKDLR+RIN+PR
Sbjct: 61  KAYNPSSCIEMAASEFPL-SIIKTTNEEHFLQSFLKKSMPKVINTIERAKDLRQRINSPR 120

Query: 264 GEAALADCVELMEISMDRITDSILALERRRCGTIENAHTWLSSVLTNHVACSDEIENSIS 323
           GE AL DC+ELMEIS  RI DS+LAL+ R  G IE++HTWLSSVLTNHV C DE+E+S+S
Sbjct: 121 GEGALDDCIELMEISNGRIMDSVLALKNRTSGYIESSHTWLSSVLTNHVTCWDEVESSLS 180

Query: 324 KAAAMDLGVEELIVIGRNSLGMLVSIWGSG-------KKEGMGYPSWLKKGDRRLLEVVG 383
           +AAAMDLG+EELI+ GRNSLGMLVSIWG          K G GYPSWLKKGDRRLL V+G
Sbjct: 181 RAAAMDLGLEELIMRGRNSLGMLVSIWGLEIKNLGELGKRGNGYPSWLKKGDRRLLGVLG 240

Query: 384 RGMKADIVVAKDGSGNFKTVKEAVEAVPDKSKSRIVIYVKKGIYKENVEVGKNKKNVMLV 443
           R M+ +IVVAKDGSGNFKTV+EAVE+VPDKSK+RIVIYVKKG YKENVEVGK KKNVM+V
Sbjct: 241 REMEPNIVVAKDGSGNFKTVQEAVESVPDKSKNRIVIYVKKGTYKENVEVGKKKKNVMIV 300

Query: 444 GDGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAEPEKHQAVALRVGAD 503
           GDGMDSTIIT SLNVVDGSTTFKSATVAAVGDGFIAQDIWF+NTA P KHQAVALRVGAD
Sbjct: 301 GDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPAKHQAVALRVGAD 360

Query: 504 QSVINRCRIDAYQDTLYAHTNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQA 563
           QSVINRCRIDAYQDTLY H+NRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPM+NQA
Sbjct: 361 QSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQA 420

Query: 564 NMVTAQGRTDPNQNTGTSIQRCEIVASPDLEPVKKSIKTYLGRPWKEYSRTVVMQSTLGD 623
           NMVTAQGR DPNQNTGTSIQ+C+IVAS DLEPVKKSIKTYLGRPWKEYSRTVVMQS +GD
Sbjct: 421 NMVTAQGRIDPNQNTGTSIQQCDIVASFDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIGD 480

Query: 624 LIDPAGWAEWNGEFALKTLYYGEYSNNGPGSNVSKRVKWPGYHVITSPAVAQKFTVDSLI 683
           LI PAGWAEW+G+FALKTLYYGEYSN GPGS+VSKRVKW GYHVITSP+ AQKFTV+SLI
Sbjct: 481 LIQPAGWAEWSGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHVITSPSEAQKFTVNSLI 540

Query: 684 QGAEWLGSSGVTFIPGL 693
           QG EWLG SG +FIPGL
Sbjct: 541 QGGEWLGPSGASFIPGL 555

BLAST of CmaCh07G002990.1 vs. NCBI nr
Match: gi|703117843|ref|XP_010101471.1| (Pectinesterase/pectinesterase inhibitor [Morus notabilis])

HSP 1 Score: 663.3 bits (1710), Expect = 4.6e-187
Identity = 343/555 (61.80%), Postives = 415/555 (74.77%), Query Frame = 1

Query: 144 MATHEPLLPQPPETTRPKTFFTILFFSLLLGSAIFITNKLINSNPSENDHHPFLHNICYK 203
           MAT++PLL     +     +  ++  + L+ +A F  + +  ++ S   + P LH IC +
Sbjct: 1   MATNQPLLETSKSSHSKIPYLLLISIAALVSTAFFAIHSIKTTSLSTPPNLP-LHKICDQ 60

Query: 204 AYNQSSCIQMLASEFPLLQETKTTNAANFLQSFLRKSTSKIVNTIESAKDLRRRINNPRG 263
           + ++ SC+ M++      Q +   +  + L++FL K+T +I   ++ A D+R RINNPR 
Sbjct: 61  SVDKESCLAMVSEA--ARQYSTVADHHDLLKAFLEKTTPRIQQALKMANDVRLRINNPRD 120

Query: 264 EAALADCVELMEISMDRITDSI--LALERRRCGTIENAHTWLSSVLTNHVACSDEIENSI 323
            A L DC ELM+IS DR+ DSI  L L+++   + E+ H WLSSVLTNHV C D +E+  
Sbjct: 121 RATLLDCAELMDISKDRVVDSISTLFLQKQTSHSHEDVHAWLSSVLTNHVTCLDALEDGS 180

Query: 324 SKAAAMDLGVEELIVIGRNSLGMLVSIWGSGKKE----GMGYPSWLKKGDRRLLEVVGRG 383
                MD  + ELI+  R SL +LVS++ S  +         P+W+  GDRRLL+   + 
Sbjct: 181 YAKNLMDSHLNELILRARTSLAILVSVFPSNSEAIETLTDDLPTWVTAGDRRLLQASVKA 240

Query: 384 MKADIVVAKDGSGNFKTVKEAVEAVPDKSKSRIVIYVKKGIYKENVEVGKNKKNVMLVGD 443
           +KADIVVA+DGSG +KTVKEAV AVPD SK+R  IYVKKG YKENVE+GK KKNVMLVGD
Sbjct: 241 IKADIVVAEDGSGKYKTVKEAVAAVPDNSKTRFTIYVKKGTYKENVEIGKKKKNVMLVGD 300

Query: 444 GMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAEPEKHQAVALRVGADQS 503
           GMDSTIIT SLNV+DGSTTF SATVAAVGDGFIAQDIWF+NTA P+KHQAVALRVGADQS
Sbjct: 301 GMDSTIITGSLNVIDGSTTFNSATVAAVGDGFIAQDIWFQNTAGPQKHQAVALRVGADQS 360

Query: 504 VINRCRIDAYQDTLYAHTNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQANM 563
           VINRCRIDAYQDTLYAH+NRQFYRDST+TGTVDFIFGNAAVV QNCK+  R+ MS Q NM
Sbjct: 361 VINRCRIDAYQDTLYAHSNRQFYRDSTVTGTVDFIFGNAAVVFQNCKLVARKGMSGQKNM 420

Query: 564 VTAQGRTDPNQNTGTSIQRCEIVASPDLEPVKKSIKTYLGRPWKEYSRTVVMQSTLGDLI 623
           VTAQGRTDPNQNTGTSIQ+CEI AS DLEPVK S K+YLGRPWKEYSRTVVMQST+GDLI
Sbjct: 421 VTAQGRTDPNQNTGTSIQKCEISASSDLEPVKASYKSYLGRPWKEYSRTVVMQSTIGDLI 480

Query: 624 DPAGWAEWNGEFALKTLYYGEYSNNGPGSNVSKRVKWPGYHVITSPAVAQKFTVDSLIQG 683
           DPAGWAEW+ +FALKTLYYGEY+N GPG+  SKRV W GYHVITSP+ AQKFTV  LIQG
Sbjct: 481 DPAGWAEWDKDFALKTLYYGEYANQGPGAGTSKRVNWAGYHVITSPSEAQKFTVAELIQG 540

Query: 684 AEWLGSSGVTFIPGL 693
             WL +SGV +  GL
Sbjct: 541 GYWLKNSGVAYTEGL 552

BLAST of CmaCh07G002990.1 vs. NCBI nr
Match: gi|568827281|ref|XP_006467993.1| (PREDICTED: pectinesterase-like [Citrus sinensis])

HSP 1 Score: 661.0 bits (1704), Expect = 2.3e-186
Identity = 347/547 (63.44%), Postives = 408/547 (74.59%), Query Frame = 1

Query: 155 PETTRPKTFFTILFFSLLLGSAIFITNKLINSNPSENDHHPFLHNICYKAYNQSSCIQML 214
           P+ ++ K    IL    ++ SA F+    I +  S N  H  LH  C KA +QSSCI M+
Sbjct: 15  PKASKRKILLLILSVVSVMCSATFVAIHCIKAINSNN--HWSLHQFCAKAQDQSSCIAMV 74

Query: 215 ASEFPLLQETKTTNAANFLQSFLRKSTSKIVNTIESAKDLRRRINNPRGEAALADCVELM 274
            SE   +  T   N  + L  FL KSTS I  TIE A  ++ RIN+PRG+AAL DCVEL+
Sbjct: 75  -SETTSINATTKMNEVDLLLIFLSKSTSNIQETIEVANHVKHRINSPRGQAALTDCVELL 134

Query: 275 EISMDRITDSILALERRRCGTIENAHTWLSSVLTNHVACSDEIENSISKAAAMDLGVEEL 334
           E+S+DRITDS+ AL++RR  + E+AH WLS VLTNHV C D ++ S +K   M+  + EL
Sbjct: 135 ELSVDRITDSMAALKKRRTSSNEDAHAWLSGVLTNHVTCLDGLDGSATKL--MEPRLVEL 194

Query: 335 IVIGRNSLGMLVSIWGSGK--------KEGMGYPSWLKKGDRRLLEVV-GRGMKADIVVA 394
           +    NSL +LV+I             ++ + +PSW+   DR LL        KA++VVA
Sbjct: 195 MARAANSLAILVAISPYNTNVIQQYYLRKEIQFPSWVTSRDRLLLRPSQAEAAKANVVVA 254

Query: 395 KDGSGNFKTVKEAVEAVPDKSKSRIVIYVKKGIYKENVEVGKNKKNVMLVGDGMDSTIIT 454
           KDGSGN+KTVKEAV + PD SK+R VIYVKKG YKENVEVGK KKN+M+VGDGMD TIIT
Sbjct: 255 KDGSGNYKTVKEAVASAPDNSKTRYVIYVKKGTYKENVEVGKKKKNLMIVGDGMDLTIIT 314

Query: 455 ASLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAEPEKHQAVALRVGADQSVINRCRID 514
            SLNVVDGSTTFKSAT+A  GDGFIAQD+W +NTA PEKHQAVALRV ADQSVINRCRID
Sbjct: 315 GSLNVVDGSTTFKSATIAVGGDGFIAQDLWIQNTAGPEKHQAVALRVSADQSVINRCRID 374

Query: 515 AYQDTLYAHTNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQANMVTAQGRTD 574
           AYQDTLYAHTNRQFYRD  ITGTVDFIFGNAAVVLQNCKI  R+PMS Q+NMVTAQGRTD
Sbjct: 375 AYQDTLYAHTNRQFYRDCDITGTVDFIFGNAAVVLQNCKIAARKPMSKQSNMVTAQGRTD 434

Query: 575 PNQNTGTSIQRCEIVASPDLEPVKKSIKTYLGRPWKEYSRTVVMQSTLGDLIDPAGWAEW 634
           PNQNTGTSIQ+CE++AS DLEPVK SI++YLGRPWKEYSRTVVMQS +GD IDPAGW+EW
Sbjct: 435 PNQNTGTSIQKCEVIASSDLEPVKGSIRSYLGRPWKEYSRTVVMQSHIGDHIDPAGWSEW 494

Query: 635 NGEFALKTLYYGEYSNNGPGSNVSKRVKWPGYHVITSPAVAQKFTVDSLIQGAEWLGSSG 693
           +G+FALKTLYYGEY N GPG+  SKRVKWPGYHVIT P  A+KFTV  LI G  WL S+G
Sbjct: 495 SGDFALKTLYYGEYLNRGPGAGTSKRVKWPGYHVITDPEEAKKFTVAELIGGGSWLKSTG 554

BLAST of CmaCh07G002990.1 vs. NCBI nr
Match: gi|641856767|gb|KDO75533.1| (hypothetical protein CISIN_1g008722mg [Citrus sinensis])

HSP 1 Score: 659.4 bits (1700), Expect = 6.7e-186
Identity = 346/547 (63.25%), Postives = 408/547 (74.59%), Query Frame = 1

Query: 155 PETTRPKTFFTILFFSLLLGSAIFITNKLINSNPSENDHHPFLHNICYKAYNQSSCIQML 214
           P+ ++ K    IL    ++ SA F+    I +  S N  H  LH  C KA +QSSCI M+
Sbjct: 15  PKASKRKILLLILSVVSVMCSATFVAIHCIKAINSNN--HWSLHQFCAKAQDQSSCIAMV 74

Query: 215 ASEFPLLQETKTTNAANFLQSFLRKSTSKIVNTIESAKDLRRRINNPRGEAALADCVELM 274
            SE   +  T   N  + L  FL KSTS I  TIE A  ++ RIN+PRG+AAL DCVEL+
Sbjct: 75  -SETTSINATTKMNEVDLLLIFLSKSTSNIQETIEVANHVKHRINSPRGQAALTDCVELL 134

Query: 275 EISMDRITDSILALERRRCGTIENAHTWLSSVLTNHVACSDEIENSISKAAAMDLGVEEL 334
           E+S+DRITDS+ AL++RR  + E+AH WLS VLTNHV C D ++ S +K   M+  + EL
Sbjct: 135 ELSVDRITDSMAALKKRRTSSNEDAHAWLSGVLTNHVTCLDGLDGSATKL--MEPRLVEL 194

Query: 335 IVIGRNSLGMLVSIWGSGK--------KEGMGYPSWLKKGDRRLLEVV-GRGMKADIVVA 394
           +    NSL +LV+I             ++ + +PSW+   DR LL        KA++VVA
Sbjct: 195 MARAANSLAILVAISPYNTNVIQQYYLRKEIQFPSWVTSRDRLLLRPSQAEAAKANVVVA 254

Query: 395 KDGSGNFKTVKEAVEAVPDKSKSRIVIYVKKGIYKENVEVGKNKKNVMLVGDGMDSTIIT 454
           KDGSGN+KTVKEAV + PD SK+R VIYVKKG YKENVEVGK KKN+M+VGDGMD TIIT
Sbjct: 255 KDGSGNYKTVKEAVASAPDNSKTRYVIYVKKGTYKENVEVGKKKKNLMIVGDGMDLTIIT 314

Query: 455 ASLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAEPEKHQAVALRVGADQSVINRCRID 514
            SLNVVDGSTTFKSAT+A  GDGFIAQD+W +NTA PEKHQAVALRV ADQSVINRCRID
Sbjct: 315 GSLNVVDGSTTFKSATIAVGGDGFIAQDLWIQNTAGPEKHQAVALRVSADQSVINRCRID 374

Query: 515 AYQDTLYAHTNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQANMVTAQGRTD 574
           AYQDTLYAHTNRQFYRD  ITGTVDFIFGNAAVVLQNCKI  R+PMS Q+NMVTAQGRTD
Sbjct: 375 AYQDTLYAHTNRQFYRDCYITGTVDFIFGNAAVVLQNCKIAARKPMSKQSNMVTAQGRTD 434

Query: 575 PNQNTGTSIQRCEIVASPDLEPVKKSIKTYLGRPWKEYSRTVVMQSTLGDLIDPAGWAEW 634
           PNQNTGTSIQ+C+++AS DLEPVK SI++YLGRPWKEYSRTVVMQS +GD IDPAGW+EW
Sbjct: 435 PNQNTGTSIQKCDVIASSDLEPVKGSIRSYLGRPWKEYSRTVVMQSHIGDHIDPAGWSEW 494

Query: 635 NGEFALKTLYYGEYSNNGPGSNVSKRVKWPGYHVITSPAVAQKFTVDSLIQGAEWLGSSG 693
           +G+FALKTLYYGEY N GPG+  SKRVKWPGYHVIT P  A+KFTV  LI G  WL S+G
Sbjct: 495 SGDFALKTLYYGEYLNRGPGAGTSKRVKWPGYHVITDPEEAKKFTVAELIGGGSWLKSTG 554