CmaCh06G001410.1 (mRNA) Cucurbita maxima (Rimu)

NameCmaCh06G001410.1
TypemRNA
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionProtease inhibitor protein
LocationCma_Chr06 : 716855 .. 717553 (-)
Sequence length612
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGAACAAAGCAAGAAACTCCAAAAGTCAGGCAGCCACTCCATCGTCAAAGATGCTTATCCCATTTTTATCCCATGAACAAAGCAAGAAACTCCAAACTTGTAAGTTCAAAGTTCATGAAATGGCTATAAATACTCTCCCCTCTAACCCATATTCAAACCCAAATCAAAGAACACCAAATCTAAGAGATCAAGATGTCTGAATGTCCTCCAGGTATGTGTTATTTTGTGCGCTTGATTTAGTTCTATCTTCTTCAAAGATTATGGAATAATTTTTTGTAATGTCCTAAATAAACGGTAGGAAAAGACCAATGGCCCGAGCTGGTTTTCGTAGAGTATTCTACTGCTGCACTGATCATTGAGAAGGAGAATCCTAACGTGAAAGCACACAAGATTCTAGCTGGGAGTCCGAGAATTTTGAACTTTGATTGTTCACGAGTTTGGGTTGATGTCAATATCAATGATGTTGTTGTTAAAGTGCCTGCGATTGGTTAAGGATTGTGAATTGATATCTATATGTAATACATACATATATTTCAAATTAATAATAAGGTAGTATCTTTCTACCACTTTCATTTCAATGATCTCTTGAGCAGGATCAAGTTAAAAGGGTAATACATACATACATTTGCGTTATCACCATGAGAAAACTTGAAATTGTGACTGAAAAACTAAGAACAAAAGATGAACAATATGACTATT

mRNA sequence

TGAACAAAGCAAGAAACTCCAAAAGTCAGGCAGCCACTCCATCGTCAAAGATGCTTATCCCATTTTTATCCCATGAACAAAGCAAGAAACTCCAAACTTGTAAGTTCAAAGTTCATGAAATGGCTATAAATACTCTCCCCTCTAACCCATATTCAAACCCAAATCAAAGAACACCAAATCTAAGAGATCAAGATGTCTGAATGTCCTCCAGGAAAAGACCAATGGCCCGAGCTGGTTTTCGTAGAGTATTCTACTGCTGCACTGATCATTGAGAAGGAGAATCCTAACGTGAAAGCACACAAGATTCTAGCTGGGAGTCCGAGAATTTTGAACTTTGATTGTTCACGAGTTTGGGTTGATGTCAATATCAATGATGTTGTTGTTAAAGTGCCTGCGATTGGTTAAGGATTGTGAATTGATATCTATATGTAATACATACATATATTTCAAATTAATAATAAGGTAGTATCTTTCTACCACTTTCATTTCAATGATCTCTTGAGCAGGATCAAGTTAAAAGGGTAATACATACATACATTTGCGTTATCACCATGAGAAAACTTGAAATTGTGACTGAAAAACTAAGAACAAAAGATGAACAATATGACTATT

Coding sequence (CDS)

ATGTCTGAATGTCCTCCAGGAAAAGACCAATGGCCCGAGCTGGTTTTCGTAGAGTATTCTACTGCTGCACTGATCATTGAGAAGGAGAATCCTAACGTGAAAGCACACAAGATTCTAGCTGGGAGTCCGAGAATTTTGAACTTTGATTGTTCACGAGTTTGGGTTGATGTCAATATCAATGATGTTGTTGTTAAAGTGCCTGCGATTGGTTAA

Protein sequence

MSECPPGKDQWPELVFVEYSTAALIIEKENPNVKAHKILAGSPRILNFDCSRVWVDVNINDVVVKVPAIG
BLAST of CmaCh06G001410.1 vs. Swiss-Prot
Match: ITH5_CUCMA (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima PE=1 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 1.8e-09
Identity = 34/69 (49.28%), Postives = 42/69 (60.87%), Query Frame = 1

Query: 2  SECPPGKDQWPELVFVEYSTAALIIEKENPNVKAHKILAGSPRILNFDCSRVWVDVNIND 61
          S CP GK  WP LV V  S A  IIE++NPNVKA  +  G+P   +F C+RV + VN   
Sbjct: 1  SSCP-GKSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRG 60

Query: 62 VVVKVPAIG 71
          +VV  P IG
Sbjct: 61 LVVSPPRIG 68

BLAST of CmaCh06G001410.1 vs. Swiss-Prot
Match: ICI_LINUS (Proteinase inhibitor OS=Linum usitatissimum PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 8.7e-09
Identity = 31/64 (48.44%), Postives = 38/64 (59.38%), Query Frame = 1

Query: 6  PGKDQWPELVFVEYSTAALIIEKENPNVKAHKILAGSPRILNFDCSRVWVDVNINDVVVK 65
          PGK+ WPELV    + AA  +E+EN NV A  +  GS    +F C RVWV VN + VV  
Sbjct: 5  PGKNAWPELVGKSGNMAAATVERENRNVHAIVLKEGSAMTKDFRCDRVWVIVNDHGVVTS 64

Query: 66 VPAI 70
          VP I
Sbjct: 65 VPHI 68

BLAST of CmaCh06G001410.1 vs. Swiss-Prot
Match: ATSI_AMACA (Trypsin/subtilisin inhibitor OS=Amaranthus caudatus PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 3.3e-08
Identity = 32/67 (47.76%), Postives = 40/67 (59.70%), Query Frame = 1

Query: 3  ECPPGKDQWPELVFVEYSTAALIIEKENPNVKAHKILAGSPRILNFDCSRVWVDVNINDV 62
          ECP GK +WPELV      AA IIE+ENPNV++      S    +F C RVWV V+   V
Sbjct: 3  ECP-GKQEWPELVGEYGYKAAAIIERENPNVRSIVKHERSGFTKDFRCDRVWVVVDSTGV 62

Query: 63 VVKVPAI 70
          VV+ P +
Sbjct: 63 VVRTPRV 68

BLAST of CmaCh06G001410.1 vs. Swiss-Prot
Match: BGIA_MOMCH (Glu S.griseus protease inhibitor OS=Momordica charantia PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 9.6e-08
Identity = 30/69 (43.48%), Postives = 40/69 (57.97%), Query Frame = 1

Query: 2  SECPPGKDQWPELVFVEYSTAALIIEKENPNVKAHKILAGSPRILNFDCSRVWVDVNIND 61
          S+C  GK  WP+LV    + A  +IE+ENP V+A  +  GSP   +F C RV V V    
Sbjct: 1  SQCQ-GKRSWPQLVGSTGAAAKAVIERENPRVRAVIVRVGSPVTADFRCDRVRVWVTERG 60

Query: 62 VVVKVPAIG 71
          +V + PAIG
Sbjct: 61 IVARPPAIG 68

BLAST of CmaCh06G001410.1 vs. Swiss-Prot
Match: HPI_HEVBR (Protease inhibitor HPI OS=Hevea brasiliensis GN=PI1 PE=1 SV=2)

HSP 1 Score: 55.8 bits (133), Expect = 2.1e-07
Identity = 33/69 (47.83%), Postives = 42/69 (60.87%), Query Frame = 1

Query: 2  SECPPGKDQWPELVFVEYSTAALIIEKENPNVKAHKILAGSPRILNFDCSRVWVDVNIND 61
          S+CP  K+ WPELV      AA II+ EN NVKA  +  G P   + + +RV V V+ N 
Sbjct: 3  SQCPV-KNSWPELVGTNGDIAAGIIQTENANVKAIVVKEGLPITQDLNFNRVRVFVDENR 62

Query: 62 VVVKVPAIG 71
          VV +VPAIG
Sbjct: 63 VVTQVPAIG 70

BLAST of CmaCh06G001410.1 vs. TrEMBL
Match: A0A0A0L8E6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G142910 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 2.2e-19
Identity = 46/68 (67.65%), Postives = 57/68 (83.82%), Query Frame = 1

Query: 3  ECPPGKDQWPELVFVEYSTAALIIEKENPNVKAHKILAGSPRILNFDCSRVWVDVNINDV 62
          E  P K +WPELVFV+YS AA IIEKENP+V+A KIL+G+PRILNFD +RVWVD+N+ +V
Sbjct: 4  ESAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDINVEEV 63

Query: 63 VVKVPAIG 71
          VVK P +G
Sbjct: 64 VVKKPKVG 71

BLAST of CmaCh06G001410.1 vs. TrEMBL
Match: A0A0A0L785_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G141900 PE=4 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 4.6e-17
Identity = 45/63 (71.43%), Postives = 51/63 (80.95%), Query Frame = 1

Query: 8  KDQWPELVFVEYSTAALIIEKENPNVKAHKILAGSPRILNFDCSRVWVDVNINDVVVKVP 67
          K +WPELVFV + TAA IIEKENP+VKA KIL  SPRI NFD SRVWVD NI + VVKVP
Sbjct: 9  KTRWPELVFVNFCTAARIIEKENPDVKAIKILVDSPRIQNFDISRVWVDCNIEERVVKVP 68

Query: 68 AIG 71
          ++G
Sbjct: 69 SVG 71

BLAST of CmaCh06G001410.1 vs. TrEMBL
Match: A0A0A0L9Z4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G142410 PE=4 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 2.4e-13
Identity = 40/63 (63.49%), Postives = 46/63 (73.02%), Query Frame = 1

Query: 8  KDQWPELVFVEYSTAALIIEKENPNVKAHKILAGSPRILNFDCSRVWVDVNINDVVVKVP 67
          K  WPELV V++S AA IIE ENPNVKA KIL GSP ILN+D +RVWV  N  D VV +P
Sbjct: 9  KSIWPELVGVDFSAAARIIETENPNVKATKILIGSPVILNYDPTRVWVICNTEDKVVDIP 68

Query: 68 AIG 71
           +G
Sbjct: 69 RVG 71

BLAST of CmaCh06G001410.1 vs. TrEMBL
Match: A0A0A0KME7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G286040 PE=4 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 5.3e-13
Identity = 39/68 (57.35%), Postives = 51/68 (75.00%), Query Frame = 1

Query: 4  CPPGKDQWPELVFVEYSTAALIIEKENPNVK-AHKILAGSPRILNFDCSRVWVDVNINDV 63
          CPPGK+QWPELV V+ +TA  II+K+NPNV+    +LAGS    +  C RVWV VNI+D+
Sbjct: 5  CPPGKNQWPELVGVKATTAKYIIKKDNPNVENVVVLLAGSGTTEDIRCDRVWVFVNIHDL 64

Query: 64 VVKVPAIG 71
          VV+VP +G
Sbjct: 65 VVEVPKVG 72

BLAST of CmaCh06G001410.1 vs. TrEMBL
Match: A0A0K9RN96_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_047410 PE=4 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 3.8e-11
Identity = 37/69 (53.62%), Postives = 46/69 (66.67%), Query Frame = 1

Query: 2  SECPPGKDQWPELVFVEYSTAALIIEKENPNVKAHKILAGSPRILNFDCSRVWVDVNIND 61
          S+C PGK++WPELV +    AA IIE ENPNV A  +  GSP   +FDC RVWV V+ + 
Sbjct: 3  SDC-PGKNEWPELVGLNGEVAAQIIESENPNVHAIVLEDGSPTTRDFDCDRVWVWVDTDG 62

Query: 62 VVVKVPAIG 71
          VVV+ P  G
Sbjct: 63 VVVRAPTAG 70

BLAST of CmaCh06G001410.1 vs. TAIR10
Match: AT2G38870.1 (AT2G38870.1 Serine protease inhibitor, potato inhibitor I-type family protein)

HSP 1 Score: 63.5 bits (153), Expect = 5.8e-11
Identity = 34/69 (49.28%), Postives = 42/69 (60.87%), Query Frame = 1

Query: 2  SECPPGKDQWPELVFVEYSTAALIIEKENPNVKAHKILAGSPRILNFDCSRVWVDVNIND 61
          +ECP  K+ WPEL       AA++IE+ENP V A  IL GSP   +F C RV V V+ N 
Sbjct: 3  TECPR-KNSWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNR 62

Query: 62 VVVKVPAIG 71
          +VVK P  G
Sbjct: 63 IVVKTPKSG 70

BLAST of CmaCh06G001410.1 vs. TAIR10
Match: AT5G43580.1 (AT5G43580.1 Serine protease inhibitor, potato inhibitor I-type family protein)

HSP 1 Score: 58.9 bits (141), Expect = 1.4e-09
Identity = 31/65 (47.69%), Postives = 41/65 (63.08%), Query Frame = 1

Query: 6  PGKDQWPELVFVEYSTAALIIEKENPNVKAHKILAGSPRILNFDCSRVWVDVNINDVVVK 65
          PGK  WPEL+  +   A  +IE+ENP +KA  IL G+     F CSRV+V VN   +VV+
Sbjct: 35 PGKSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVNDCGIVVQ 94

Query: 66 VPAIG 71
          +P IG
Sbjct: 95 IPIIG 99

BLAST of CmaCh06G001410.1 vs. NCBI nr
Match: gi|700201764|gb|KGN56897.1| (hypothetical protein Csa_3G142910 [Cucumis sativus])

HSP 1 Score: 102.4 bits (254), Expect = 3.2e-19
Identity = 46/68 (67.65%), Postives = 57/68 (83.82%), Query Frame = 1

Query: 3  ECPPGKDQWPELVFVEYSTAALIIEKENPNVKAHKILAGSPRILNFDCSRVWVDVNINDV 62
          E  P K +WPELVFV+YS AA IIEKENP+V+A KIL+G+PRILNFD +RVWVD+N+ +V
Sbjct: 4  ESAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDINVEEV 63

Query: 63 VVKVPAIG 71
          VVK P +G
Sbjct: 64 VVKKPKVG 71

BLAST of CmaCh06G001410.1 vs. NCBI nr
Match: gi|700201761|gb|KGN56894.1| (hypothetical protein Csa_3G141900 [Cucumis sativus])

HSP 1 Score: 94.7 bits (234), Expect = 6.6e-17
Identity = 45/63 (71.43%), Postives = 51/63 (80.95%), Query Frame = 1

Query: 8  KDQWPELVFVEYSTAALIIEKENPNVKAHKILAGSPRILNFDCSRVWVDVNINDVVVKVP 67
          K +WPELVFV + TAA IIEKENP+VKA KIL  SPRI NFD SRVWVD NI + VVKVP
Sbjct: 9  KTRWPELVFVNFCTAARIIEKENPDVKAIKILVDSPRIQNFDISRVWVDCNIEERVVKVP 68

Query: 68 AIG 71
          ++G
Sbjct: 69 SVG 71

BLAST of CmaCh06G001410.1 vs. NCBI nr
Match: gi|700201763|gb|KGN56896.1| (hypothetical protein Csa_3G142410 [Cucumis sativus])

HSP 1 Score: 82.4 bits (202), Expect = 3.4e-13
Identity = 40/63 (63.49%), Postives = 46/63 (73.02%), Query Frame = 1

Query: 8  KDQWPELVFVEYSTAALIIEKENPNVKAHKILAGSPRILNFDCSRVWVDVNINDVVVKVP 67
          K  WPELV V++S AA IIE ENPNVKA KIL GSP ILN+D +RVWV  N  D VV +P
Sbjct: 9  KSIWPELVGVDFSAAARIIETENPNVKATKILIGSPVILNYDPTRVWVICNTEDKVVDIP 68

Query: 68 AIG 71
           +G
Sbjct: 69 RVG 71

BLAST of CmaCh06G001410.1 vs. NCBI nr
Match: gi|700195663|gb|KGN50840.1| (hypothetical protein Csa_5G286040 [Cucumis sativus])

HSP 1 Score: 81.3 bits (199), Expect = 7.6e-13
Identity = 39/68 (57.35%), Postives = 51/68 (75.00%), Query Frame = 1

Query: 4  CPPGKDQWPELVFVEYSTAALIIEKENPNVK-AHKILAGSPRILNFDCSRVWVDVNINDV 63
          CPPGK+QWPELV V+ +TA  II+K+NPNV+    +LAGS    +  C RVWV VNI+D+
Sbjct: 5  CPPGKNQWPELVGVKATTAKYIIKKDNPNVENVVVLLAGSGTTEDIRCDRVWVFVNIHDL 64

Query: 64 VVKVPAIG 71
          VV+VP +G
Sbjct: 65 VVEVPKVG 72

BLAST of CmaCh06G001410.1 vs. NCBI nr
Match: gi|720071522|ref|XP_010278088.1| (PREDICTED: inhibitor of trypsin and hageman factor-like [Nelumbo nucifera])

HSP 1 Score: 78.2 bits (191), Expect = 6.4e-12
Identity = 40/69 (57.97%), Postives = 45/69 (65.22%), Query Frame = 1

Query: 2  SECPPGKDQWPELVFVEYSTAALIIEKENPNVKAHKILAGSPRILNFDCSRVWVDVNIND 61
          SEC  GK  WPELV  E   A   IE+ENP V A  +L G+P ILNFDC+RVWV V+ N 
Sbjct: 3  SECS-GKSSWPELVGAEGEVAKATIEQENPAVTAIIVLEGTPVILNFDCTRVWVWVDTNG 62

Query: 62 VVVKVPAIG 71
           V KVP IG
Sbjct: 63 TVTKVPKIG 70

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ITH5_CUCMA1.8e-0949.28Inhibitor of trypsin and hageman factor OS=Cucurbita maxima PE=1 SV=1[more]
ICI_LINUS8.7e-0948.44Proteinase inhibitor OS=Linum usitatissimum PE=1 SV=1[more]
ATSI_AMACA3.3e-0847.76Trypsin/subtilisin inhibitor OS=Amaranthus caudatus PE=1 SV=1[more]
BGIA_MOMCH9.6e-0843.48Glu S.griseus protease inhibitor OS=Momordica charantia PE=1 SV=1[more]
HPI_HEVBR2.1e-0747.83Protease inhibitor HPI OS=Hevea brasiliensis GN=PI1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0L8E6_CUCSA2.2e-1967.65Uncharacterized protein OS=Cucumis sativus GN=Csa_3G142910 PE=4 SV=1[more]
A0A0A0L785_CUCSA4.6e-1771.43Uncharacterized protein OS=Cucumis sativus GN=Csa_3G141900 PE=4 SV=1[more]
A0A0A0L9Z4_CUCSA2.4e-1363.49Uncharacterized protein OS=Cucumis sativus GN=Csa_3G142410 PE=4 SV=1[more]
A0A0A0KME7_CUCSA5.3e-1357.35Uncharacterized protein OS=Cucumis sativus GN=Csa_5G286040 PE=4 SV=1[more]
A0A0K9RN96_SPIOL3.8e-1153.62Uncharacterized protein OS=Spinacia oleracea GN=SOVF_047410 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G38870.15.8e-1149.28 Serine protease inhibitor, potato inhibitor I-type family protein[more]
AT5G43580.11.4e-0947.69 Serine protease inhibitor, potato inhibitor I-type family protein[more]
Match NameE-valueIdentityDescription
gi|700201764|gb|KGN56897.1|3.2e-1967.65hypothetical protein Csa_3G142910 [Cucumis sativus][more]
gi|700201761|gb|KGN56894.1|6.6e-1771.43hypothetical protein Csa_3G141900 [Cucumis sativus][more]
gi|700201763|gb|KGN56896.1|3.4e-1363.49hypothetical protein Csa_3G142410 [Cucumis sativus][more]
gi|700195663|gb|KGN50840.1|7.6e-1357.35hypothetical protein Csa_5G286040 [Cucumis sativus][more]
gi|720071522|ref|XP_010278088.1|6.4e-1257.97PREDICTED: inhibitor of trypsin and hageman factor-like [Nelumbo nucifera][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR000864Prot_inh_pot1
Vocabulary: Molecular Function
TermDefinition
GO:0004867serine-type endopeptidase inhibitor activity
Vocabulary: Biological Process
TermDefinition
GO:0009611response to wounding
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009611 response to wounding
biological_process GO:0010951 negative regulation of endopeptidase activity
cellular_component GO:0005575 cellular_component
molecular_function GO:0004867 serine-type endopeptidase inhibitor activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CmaCh06G001410CmaCh06G001410gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CmaCh06G001410.1CmaCh06G001410.1-proteinpolypeptide


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh06G001410.1.three_prime_UTR.1CmaCh06G001410.1.three_prime_UTR.1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh06G001410.1.CDS.2CmaCh06G001410.1.CDS.2CDS
CmaCh06G001410.1.CDS.1CmaCh06G001410.1.CDS.1CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh06G001410.1.five_prime_UTR.1CmaCh06G001410.1.five_prime_UTR.1five_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CmaCh06G001410.1.exon.2CmaCh06G001410.1.exon.2exon
CmaCh06G001410.1.exon.1CmaCh06G001410.1.exon.1exon


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000864Proteinase inhibitor I13, potato inhibitor IPRODOMPD002604coord: 10..52
score: 4.
IPR000864Proteinase inhibitor I13, potato inhibitor IPFAMPF00280potato_inhibitcoord: 8..70
score: 5.0
IPR000864Proteinase inhibitor I13, potato inhibitor IunknownSSF54654CI-2 family of serine protease inhibitorscoord: 5..70
score: 5.89
NoneNo IPR availableGENE3DG3DSA:3.30.10.10coord: 5..70
score: 1.5
NoneNo IPR availablePANTHERPTHR33091FAMILY NOT NAMEDcoord: 2..70
score: 4.1
NoneNo IPR availablePANTHERPTHR33091:SF1SUBFAMILY NOT NAMEDcoord: 2..70
score: 4.1